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Article in Chinese | WPRIM | ID: wpr-805153


Objective@#To understand the situation of ticks carrying pathogens in border areas of Heilongjiang province.@*Methods@#From 2009 to 2018, tick specimens were collected in Yichun, Daxing′anling area and Jiamusi in Heilongjiang province. A total of 2 530 ticks were studied, including 800 Ixodes persulcatus and 1 730 Dermacentor silvarum. Tick-borne encephalitis virus (TBEV), severe fever with thrombocytopenia syndrome virus (SFTSV), omsk hemorrhagic fever virus (OHFV), langat virus (LGTV), powassan virus (POWV) were detected by real-time RT-PCR. Spotted fever group rickettsia (SFGR) and Borrelia burgdorferi sensulato (B.b.s.l) were detected by PCR in ticks collected from Jiamusi area.@*Results@#All tick speciments collected were negative for TBEV, SFTSV, OHFV, LGTV and POWV. Tick specimens from Jiamusi carried SFGR and B. b.s.l.with positive rates of 59.5% and 8.9%.@*Conclusions@#The ticks in border areas of Heilongjiang province carry spotted fever group rickettsia and Borrelia burgdorferi, and the carrying rate of spotted fever group rickettsia is high. The monitoring and control of ticks and tick-borne diseases should be strengthened.

Chinese Pharmacological Bulletin ; (12): 1580-1585, 2015.
Article in Chinese | WPRIM | ID: wpr-480653


Aim To examine the inhibitory effect of re-combinant cardiac troponin fusion protein composed of subunit I and artificial peptide which was called CIS on tumor growth. Methods The CIS ’ s effect on the growth of human umbilical vein endothelial cells ( HU-VEC) was examined using MTT assay in vitro. Chick chorioallantoic membrane model was applied to study the alteration of angiogenesis treated with purified re-combinant CIS protein. The effect of tumor growth trea-ted with CIS was observed using several in vivo mice xenograft models. Results There was a statistically significant reduction in HUVEC cell proliferative rate when the cells were treated with purified CIS fusion protein, which was also shown in a dose-dependent manner. A decreased amount of new blood vessel for-mation ( angiogenesis) on chick embryo chorioallantoic membranes was observed in recombinant CIS protein treated group compared to the untreated control group. A significant inhibition of tumor growth rate was a-chieved in CIS treated mice compared to CIS untreated control mice in 6 different mouse xenograft models. Conclusions The fusion protein CIS shows the inhibi-tory effect on the tumor growth in our in vivo mouse models, and such inhibition could be mediated by the mechanism of CIS’ s effect on the decrease of HUVEC cell proliferation and further the reduction of angiogen-esis in tumor tissues. This work could provide the foundation for the in-depth investigations on the phar-maceutical application of CIS targeting anti-tumor ther-apy.

Article in Chinese | WPRIM | ID: wpr-560372


Aim To study the molecular basis of protection on myocardial ischemic injuries of total flavones of choerospondias axiaris fructus(TFC). Methods The microarray analysis for the protein expression in myocardium provide a strong tool to explore the key protein candidates involved in the pathogenesis of ischemic injury.Surface enhanced laser desorption/ionization(SELDI) mass spectrometry with protein chip IMAC3,SAX2 and NP20 was used to compare the differentially expressed protein in TFC-treated and untreated ischemic myocardium in rats and the results were analysized with Proteinchip Software 3.0.2.Results Seven differentially expressed proteins were indentified in TFC treated myocardium.These differential effects correlated with the expression of five downregulated proteins and two upregulated proteins,and four of them were discovered on the IMAC3 chip and one of them was discovered on the SAX2 chip.Conclusions The myocardial protection of TFC may be mediated by the differential expression of these proteins which could be the key protein candidates for further investigation.

Article in Chinese | WPRIM | ID: wpr-676965


In the isolated perfused rat heart, TFH counteracted significantly the arrhythmias caused by perfusing with a solution without oxygen; increased ventricular fibrillation threshold markedly and showed a good dose-effective relation; prolonged the P-R period of electrocardiogram and decreased the heart rate and fell the amplitude of cardiac contraction markedly; and counteracted significantly the decrease of the heart rate and the amplitude of cardiac contraction caused by perfusing with a solution without oxygen. In the isolated guinea pig left atrium, TFH prolonged the function refractory period slightly. In the isolated guinea pig right atrium, TFH increased the accumulated dose of aconitine inducing arrhythmias.

Article in Chinese | WPRIM | ID: wpr-550294


Typha angustifocia L(TA ) and its extracts: total flavones ( FT ) , organic acids ( OT ) , and poly saccharide ( PT ) inhibited platelet-aggregation induced by arachidonic acid in rabbits significantly and dose-dependend in vitro. The IC50-m of TA, FT, OT and PT for maximum aggregation % was 9.3, 3.0, 12.1, and 6.5g/L PRP and IC50-s for aggregating slope was 10.8, 4.3, 17.7 and 15.3 g/L PRP respectively. The ratio of IC50-m/IC50-s was TA 1 : 1.2, FT 1 : 1.4, OT 1 : 1.5, and PT I : 2.4 respectively. FT and OT also showed inhibition to platelet aggregation exvivo. The results proved that flavones may be the principal effective ingredient of Typha angustifocia L on platelet aggregation and responsible for the difference the drug effects on maximum aggregation % and aggregation slope between.