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1.
Article in English | WPRIM | ID: wpr-811199

ABSTRACT

PURPOSE: C-terminal binding protein 1 (CtBP1) is a transcriptional co-repressor that is overexpressed in many cancers. CtBP1 transcriptionally represses a broad array of tumor suppressors, which promotes cancer cell proliferation, migration, invasion, and resistance to apoptosis. Recent studies have demonstrated that CtBP1 is a potential target for cancer therapy. This study was designed to screen for compounds that potentially target CtBP1.METHODS: Using a structure-based virtual screening for CtBP1 inhibitors, we found protocatechuic aldehyde (PA), a natural compound found in the root of a traditional Chinese herb, Salvia miltiorrhiza, that directly binds to CtBP1. Microscale thermophoresis assay was performed to determine whether PA and CtBP1 directly bind to each other. Further, clustered regularly interspaced short palindromic repeats associated Cas9 nuclease-mediated CtBP1 knockout in breast cancer cells was used to validate the CtBP1 targeting specificity of PA.RESULTS: Functional studies showed that PA repressed the proliferation and migration of breast cancer cells. Furthermore, PA elevated the expression of the downstream targets of CtBP1, p21 and E-cadherin, and decreased CtBP1 binding affinity for the promoter regions of p21 and E-cadherin in breast cancer cells. However, PA did not affect the expression of p21 and E-cadherin in the CtBP1 knockout breast cancer cells. In addition, the CtBP1 knockout breast cancer cells showed resistance to PA-induced repression of proliferation and migration.CONCLUSION: Our findings demonstrated that PA directly bound to CtBP1 and inhibited the growth and migration of breast cancer cells through CtBP1 inhibition. Structural modifications of PA are further required to enhance its binding affinity and selectivity for CtBP1.


Subject(s)
Apoptosis , Asians , Breast Neoplasms , Breast , Cadherins , Carrier Proteins , Cell Proliferation , Clustered Regularly Interspaced Short Palindromic Repeats , Humans , Mass Screening , Promoter Regions, Genetic , Repression, Psychology , Salvia miltiorrhiza , Sensitivity and Specificity
2.
China Pharmacist ; (12): 246-250, 2018.
Article in Chinese | WPRIM | ID: wpr-705499

ABSTRACT

Objective:To investigate the mechanism of promoting wound healing of Shengji Huayu ointment. Methods: Db/db mice were divided into 5 groups according to the randomized block design: three Shengji Huayu ointment groups respectively at high (15 g·kg-1),middle (10 g·kg-1) and low(5 g·kg-1) dose,the positive control group(Beifuji) and the model control group, and balb/c mice were used in the normal control group,and each group was with 10 mice. The model of full-thickness skin defect in mouse skin was used,and after HE staining,IL-1β and collagen type I and type III in granulation tissue were observed by immunohis-tochemistry and image analysis. Results:HE staining showed that the wound healing was obvious in the high dose group and the mid-dle dose group. The expression of IL-1 β in each group was not obvious. The levels of collagen type I and type III in the three Shengji Huayu ointment groups were higher than those in the model control group (P<0.05).Conclusion: Shengji Huayu ointment can pro-mote wound healing and reduce scar formation in mice.

3.
China Pharmacist ; (12): 2138-2140, 2015.
Article in Chinese | WPRIM | ID: wpr-484646

ABSTRACT

Refractory wounds are the common complication of diabetes,and the therapeutic scheme and methods are paid more and more attention nowadays. The influence of traditional Chinese medicines on diabetic wound healing, such as promoting fibroblasts proliferation, enhancing granulation tissue formation, increasing the secretion of growth factors and reducing the wound scar, was re-viewed in the paper to provide scientific basis and reference for traditional Chinese medicines used in diabetic refractory wounds.

4.
Article in Chinese | WPRIM | ID: wpr-449153

ABSTRACT

To find the related syndrome factors of gouty arthritis with blood stasis syndrome by mathematical methods of set pair analysis and connection number so as to guide the diagnosis.

5.
Journal of Integrative Medicine ; (12): 1246-9, 2008.
Article in Chinese | WPRIM | ID: wpr-450131

ABSTRACT

To explore the clinical efficacy of Herba Saxifragae cream (HS), a compound of traditional Chinese herbal medicine, on chronic eczema.

6.
Journal of Integrative Medicine ; (12): 586-90, 2008.
Article in Chinese | WPRIM | ID: wpr-449233

ABSTRACT

OBJECTIVE: To explore the clinical efficacy of Qinzhu Liangxue Decoction (QZLXD), a compound traditional Chinese herbal medicine, in patients with blood-heat type psoriasis vulgaris. METHODS: Fifty-eight patients diagnosed with blood-heat type psoriasis vulgaris were randomly divided into two groups: QZLXD group (30 cases) and ampeptide group (28 cases). Patients in both groups were treated for 4 weeks. Psoriasis Area and Severity Index (PASI) score, Dermatology Life Quality Index (DLQI) score and the level of serum vascular endothelial growth factor (VEGF) were obtained to evaluate the efficacy of the two treatments. RESULTS: There was a better curative result in QZLXD group than in ampeptide group (P<0.05). The response rates in QZLXD and ampeptide groups were 83.33% and 64.28% respectively. DLQI score was also significantly improved during 4-week treatment in QZLXD and ampeptide groups (P<0.05). There was no significant difference in VEGF level between QZLXD and ampeptide groups. CONCLUSION: QZLXD is remarkably advantageous in treatment of psoriasis vulgaris of blood-heat syndrome and improvement of patient's quality of life. The mechanism may be related to decrease in serum VEGF level and action against neogenesis of blood vessels.

7.
Article in Chinese | WPRIM | ID: wpr-449388

ABSTRACT

OBJECTIVE: To study the effects of resolving stagnation and promoting granulation therapy on expressions of Bax and Bcl-2 in granulation tissue of diabetic rats during wound healing. METHODS: Seventy-two male SD diabetic rats with full-thickness skin lesion were randomly divided into 3 groups: SJHYR 1-treated group, SJHYR 2-treated group and normal saline (NS) control group. SJHYR 1 was prepared with Shengji Recipe (SJR, a compound traditional Chinese herbal medicine for promoting granulation) and Huayu Recipe (HYR, a compound traditional Chinese herbal medicine for resolving stagnation) at a ratio of 1:2, while SJHYR 2 was prepared with SJR and HYR at a ratio of 1:1. Immunohistochemical method was used to assess Bax and Bcl-2 protein levels in granulation tissue. RESULTS: SJHYR 1 could accelerate wound healing as compared with SJHYR 2 and NS (P<0.05). On the third day in experiment, Bax and Bcl-2 proteins were not found in any groups, but on the seventh and eleventh day in experiment, Bax and Bcl-2 proteins in SJHYR 1-treated group were much higher than those in the other two groups (P<0.05). CONCLUSION: SJR and HYR in different ratios may all have a role in regulating Bax and Bcl-2 expression in granulation tissue of diabetic rats during wound healing.

8.
Article in Chinese | WPRIM | ID: wpr-449735

ABSTRACT

OBJECTIVE: To study the effects of Shengji Huayu Recipe (a traditional Chinese medicine compound recipe for resolving stagnation and promoting granulation) and its decomposed formulas (Huayu Recipe for resolving stagnation and Shengji Recipe for promoting granulation) on the synthesis of collagen types I and III in granulation tissue of rats in early wound healing. METHODS: Twenty-four male Sprague-Dawley (SD) rats with full-thickness skin lesion were randomized into 4 groups: Shengji Huayu Recipe-treated group, Shengji Recipe-treated group, Huayu Recipe-treated group and untreated group. Collagen types I and III in granulation tissue of the rats were tested with immunohistochemical methods and image analysis. RESULTS: On the third day of wound healing, collagen I of the rats in both Shengji Huayu Recipe-treated group and Shengji Recipe-treated group was higher than that in the untreated group, and collagen I of the rats in Huayu Recipe-treated group was lower than that in the untreated group (P<0.05). Collagen III of the rats in the three treated groups were lower than that in the untreated group (P<0.05). On the seventh day of wound healing, Collagen I of the rats in both Shengji Huayu Recipe-treated group and Shengji Recipe-treated group was higher than that in the untreated group (P<0.05), and collagen III of the rats in both Shengji Recipe-treated group and Huayu Recipe-treated group was higher than that in the untreated group (P<0.05). CONCLUSION: Resolving stagnation and promoting granulation therapy can promote the wound healing in rats.

9.
Article in Chinese | WPRIM | ID: wpr-450016

ABSTRACT

OBJECTIVE: To observe the clinical effect of escin on patients with cutaneous pruritus caused by blood stasis and wind-dryness and to prove the theory that "wind should be treated by regulating blood disorder, and wind disappears after activating blood" in traditional Chinese medicine (TCM). METHODS: Fifty-one patients were randomly divided into escin-treated group (n=30) and loratadine-treated group (n=21). The patients in the escin-treated group were treated with escin for 4 weeks (300 mg, b.i.d.), and the patients in the loratadine-treated group were treated with loratadine (10 mg, q.d.). Symptom score reducing index (SSRI) was used to assess the pruritus degree, lesion range and lesion shape before the treatment, 2 weeks and 4 weeks after the treatment, respectively. RESULTS: Two weeks after the treatment, the effective rate of the escin-treated group was 63.3%, and the effective rate of the loratadine-treated group was 67.0%. Four weeks after the treatment, the effective rates were 86.7% and 80.0% in escin- and loratadine-treated groups respectively. There was no statistical difference in total scores of SSRI in two groups (P>0.05), and the scores of pruritus degree and lesion shape also had no statistical difference (P>0.05), while the score of lesion range of the escin-treated group was lower than that of the loratadine-treated group (P<0.05). CONCLUSION: Escin has satisfactory effect in treating pruritus caused by blood stasis and wind-dryness. The study confirms the TCM theory that "wind should be treated by regulating blood disorder, and wind disappears after activating blood".

10.
Article in Chinese | WPRIM | ID: wpr-548355

ABSTRACT

0.05).Except that the wet weight of the epididymis in high-dose group of apigenin was significantly different compared with middle-dose group of apigenin(P 0.05).Cell cycle analysis showed that compared with the negative control group,the percent of diploid cells in low and high-dose group was decreased,the proportion of G0/G1 phase cells in low-dose group of apigenin was reduced(P

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