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Journal of Gastric Cancer ; : 212-224, 2020.
Article | WPRIM | ID: wpr-835753


Purpose@#miR-205 is a tumor suppressor and plays an important role in tumor invasiveness. However, the role of miR-205 in human gastric cancer (GC) epithelial-mesenchymal transition (EMT) remains unclear. The aim of this study was to investigate the molecular mechanism of miR-205 in the regulation of EMT in GC invasion. @*Materials and Methods@#Quantitative polymerase chain reaction (qPCR) was used to detect the expression of miR-205 in GC. Further, the correlation between the pathological parameters and prognosis of GC was statistically analyzed. A transwell model was used to evaluate the effect of miR-205-3p on the invasion and migration of GC cells. qPCR, western blotting, and luciferase assay were performed to analyze the relationship and target effects between miR-205-3p and the expression of zinc finger electron box binding homologous box 1 (ZEB1) and 2 (ZEB2). @*Results@#We found that the levels of miR-205-3p were significantly lower (P<0.05) in GC tissues than in matched normal tissues. Additionally, the expression of miR-205-3p was related to the tumor invasion depth, lymph node metastasis, lymph node invasion, and tumor, node, metastasis stage. Patients with lower miR-205-3p expression levels in the tumors had a poorer prognosis. The in vitro assays indicated that miR-205-3p could affect the invasion ability and EMT of GC cells by targeting the expression of both ZEB1 and ZEB2. @*Conclusions@#miR-205-3p promotes GC progression and affects the prognosis of patients by targeting both ZEB1 and ZEB2 to directly influence EMT.

Article in Chinese | WPRIM | ID: wpr-505905


Objective To explore the treatment effect and application value of ultrasonic guided mammotome minimally invasive whirling surgery and open surgery for benign breast tumor.Methods 264 patients with benign breast diseases were randomly divided into observation group and control group,132 cases in each group.The control group took open surgery,the observation group used mammotome minimally invasive surgery,rotary cutting system.Recorded clinical surgery treatment of the two groups.Results The operating time,intraoperative blood loss,operation scar size of the observation group were (18.27 ± 3.05) min,(10.86 ± 1.69) mL,(2.76 ± 0.85) mm respectively,those of the control group were (53.48 ± 6.17) min,(97.54 ± 14.28) mL,(27.63 ± 4.31) mm respectively,the differences between the two groups were statistically significant (t =58.7753,69.2560,65.0429,all P < 0.05).In the observation group,breast deformation in 0 case,postoperative occurrence skin pigmentation in 1 case,itchy skin in 2 cases,with lateral swelling in 1 case,the patients' satisfaction score was (93.42 ± 3.89) points.In the control group,breast deformation in 5 cases,postoperative occurrence skin pigmentation in 8 cases,skin itching in 9 cases,lateral swelling in 7 cases,the patients' satisfaction score was (88.16 ± 1.45) points,the differences between the two groups were statistically significant(x2 =5.096,5.636,4.648,4.604,t =14.557,all P <0.05).Conclusion Using ultrasonic guided mammotome minimally invasive rotary surgical treatment for benign breast diseases can shorten the operation time,reduce intraoperative bleeding,reduce the incidence of postoperative complications,patients' satisfaction is higher,and it is worthy of popularization and application in clinic.

Article in Chinese | WPRIM | ID: wpr-663360


Objective To explore the diagnostic value of ultrasound-guided puncture biopsy in breast masses.Methods 50 patients were enrolled in this study for breast mass examination and ultrasound presentation of BI-RADS class Ⅳ.All the patients underwent ultrasound-guided biopsy and pathological examination.The pathological results of the biopsy were compared with the final results of pathological diagnosis.The sensitivity and specificity of ultrasound-guided biopsy,and the accuracy of each type of breast mass examination were observed.Results 50 cases of breast masses were successfully punctured under ultrasound guidance,the postoperative pathological results were the diagnostic criteria.The puncture biopsy results showed that 16 cases of benign lesions and 34 cases of non-benign lesions.The postoperative pathological examination results showed that 13 cases of benign lesions,37 cases of non-benign lesions,the sensitivity and the specificity of the biopsy was 89.19% and 92.31%.Pathological puncture results showed 16 cases of benign tumors with an accuracy rate of 81.25%,27 cases of malignant tumors,the accuracy rate was 92.59%,7 cases of high risk,the accuracy rate was 57.14%.Compared with the accuracy of the diagnosis of benign and malignant results,the diagnostic accuracy of high-risk results was the lowest(x2 =20.802,P < 0.01).Conclusion Ultrasound-guided puncture biopsy can better identify the benign and malignant nature of breast masses,with high accuracy,small trauma,it is safe and reliable,and has important clinical value for the diagnosis of breast mass,which can provide a reliable basis for further clinical treatment scheme.

Chinese Journal of Immunology ; (12): 97-102, 2015.
Article in Chinese | WPRIM | ID: wpr-458470


Objective:To investigate the Musashi 1 expression, and its correlation with clinical variables, such as clinical pathologic factors,angiogenesis and prognosis in colon cancer.Methods: Musashi 1 mRNA level was determined by quantitative real-time PCR( qRT-PCR) in 36 pairs of matched colon cancer tissues and adjacent non-tumorous tissues.The expression of Musashi 1 and mi-crovascular density ( MVD) were tested by immunohistochemical in 96 cases of colon cancer.Combined with postoperative follow-up survival situation, the correlation between the expression of Musashi 1, pathological parameters, prognosis and MVD were statistical analylised.Results:Musashi 1 mRNA level of colon cancer tissues determined by PCR was significantly higher than adjacent non-tumorous tissues( P<0.05).Positive Musashi 1 immunoreactivity was seen in 63.5%of colon cancer specimens,which was correlated with the depth of invasion,Duckes stage,lymph node metastasis,and TNM stage (P<0.05).Compared with Musashi 1 negative patients, Musashi 1 positive patients had significantly shorter survival time ( 23.0% vs 60.0%, P<0.01 ) .High intratumor blood microvessel density patients had significantly shorter survival times than low intratumor blood microvessel density patients(P<0.05).Intratumor blood microvessel density correlated with Musashi 1 expression (P<0.01).Conclusion:Musashi 1 mRNA level of colon cancer tissues was higher than adjacent non-tumorous tissues,the difference has statistically significant.We conclude that Musashi 1 expression correlates with the depth of invasion,Dukes stage,lymph node metastasis,and TNM stage and microvascular density ( MVD) in colon cancer.We propose that synthesized Musashi 1 increases intratumor angiogenesis,thus promotes tumor progression.Musashi 1 expression may be a good biomarker for poor prognosis in colon cancer.