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Article in English | LILACS, BBO | ID: biblio-1287504


ABSTRACT Objective: To compare salivary and serum biochemical levels in patients with chronic renal failure undergoing hemodialysis. Material and Methods: The sample was composed of 57 patients treated in Hemodialysis Reference Centers, from a state of Northeastern Brazilian, with age ≥21 years old with at least 3 months of hemodialysis treatment time. Serum data were obtained from records. Unstimulated and stimulated saliva were collected. Flow rate (mL/min) was measured. Spectrophotometry was performed for the measurement of salivary levels of calcium (570 nm), urea (340 nm), and creatinine (510 nm). Statistical analysis used Mann Whitney and Kruskal-Wallis tests (p<0.05). Results: Unstimulated and stimulated salivary flow rates were 0.43 mL/min and 1.69 mL/min, respectively. There was significant difference (p<0.001) of levels of calcium (5.41 mg/dL and 9.70 mg/dL), urea (118.03 mg/dL and 183.22 mg/dL) and creatinine (0.59 mg/dL and 9.20 mg/dL) between saliva and serum, respectively. Concerning the time of hemodialysis, salivary and serum calcium not exhibited significant association; however, serum urea (p=0.012) and serum creatinine (p=0.025) showed significant association to the time of hemodialysis. Conclusion: Salivary biochemical levels of urea, creatinine and calcium can indicate the presence of a possible chronic renal failure and the saliva demonstrated to be a potential auxiliary biofluid for clinical monitoring renal alterations.

Humans , Male , Female , Adult , Middle Aged , Aged , Saliva/immunology , Spectrophotometry/methods , Renal Dialysis/instrumentation , Creatinine , Renal Insufficiency, Chronic/pathology , Brazil/epidemiology , Medical Records , Calcium , Cross-Sectional Studies/methods , Statistics, Nonparametric
Rev. ABENO ; 20(1): 52-67, 2020. ilus, tab, graf
Article in Portuguese | LILACS, BBO | ID: biblio-1284603


O objetivo do presente estudo foi desenvolver e realizar a validação de conteúdo e de face de uma escala de atitudes relacionadas às competências odontológicas (EA-CO). Tratou-se de estudo de desenvolvimento metodológico envolvendo construção e validação de uma escala, com abordagem quanti e qualitativa.O referencial teórico para o construto da escala envolveu o conceito de atitude no campo da Psicologia Social, e o de competência, no campo da Educação, qualificada pelas Diretrizes Curriculares Nacionais para os cursos deOdontologia,originando os indicadores: Empatia na relação profissional-paciente, Aprendizagem ao longo da vida, Disponibilidade para aprendizagem interprofissional, Proteção aos direitos do paciente, Responsabilidade social e Inteligênciaemocional. Dez especialistas participaram da validação de face da matriz de indicadores (Técnica do Grupo Nominal) e dos itens (Questionário) e a versão preliminar foi testada empiloto com17 estudantes de Odontologia. Houve consenso para permanência dosindicadores, exceto para Inteligência emocional. A escala (tipo Likert, 5 pontos) resultou em 53 itens,sendo 13 alterados da versão original.A EA-CO foi desenvolvida e atendeu aos critérios de validação de conteúdo e de face utilizados no estudo (AU).

This study aimed to develop and validate the content and face of a scale of attitudes related to dental competencies (SA-DC).It was a methodological development study involving the design and validation of a scale, using a quantitative and qualitative approach.The theoretical framework for the construct of the scale involved the concept of attitude in the field of Social Psychology and that of competence in the field of Education, qualified by the National CurriculumGuidelines for Dentistry Programs, thus providing the following indicators: Empathy in the professional-patient relationship, Lifelong learning, Readiness for interprofessional education, Protection of patients' rights, Social responsibility and Emotional intelligence.Ten experts participated in the face validation of the matrix of indicators (Nominal Group Technique) and of the items (Questionnaire), and the preliminary version was pilot tested with 17 undergraduate dentistry students.There was consensus on the permanence of the indicators, except for Emotional Intelligence.The scale (5-point Likert type) resulted in 53 items,13 of which were modified from the original version.SA-DC was developed and met the content and face validation criteria used in the study (AU).

Humans , Male , Female , Adult , Professional Competence , Students, Dental , Attitude of Health Personnel , Validation Study , Interprofessional Education , Brazil , Surveys and Questionnaires , Education, Dental , Educational Measurement
Article in English | LILACS, BBO | ID: biblio-1135565


Abstract Objective: To perform an in vitro analysis of antibacterial and antifungal potential of an alcoholic extract from the leaves of Guapira Graciliflora Mart. against oral microorganisms and determine its chemical composition. Material and Methods: A hydroalcoholic extract of the leaves form G. graciliflora was obtained through maceration, vacuum concentration and freeze-drying. Antibacterial and antifungal activities were evaluated against Streptococcus mutans, Streptococcus salivarius, Streptococcus oralis, Streptococcus parasanguinis, Streptococcus mitis and strains of Candida albicans using broth microdilution method. Phytochemical analysis determined the total phenolic compounds, protein concentration and total of sugars present in the extract. Results: G. Graciliflora demonstrated antifungal activity against the LM 11 and LM 410 clinical isolates of C. albicans (MIC 0.5 mg/mL and 2 mg/mL, respectively). The other microorganisms tested were resistant to the extract. The phytochemical analysis revealed 3% proteins, 13% total sugars and 17% phenolic compounds. Conclusion: G. Graciliflora has antifungal activity against clinical strains of C. albicans and exhibits proteins, sugars and phenolic compounds in its chemical composition.

Plants, Medicinal , In Vitro Techniques , Plant Extracts/pharmacology , Anti-Infective Agents , Anti-Bacterial Agents , Candida albicans , Microbial Sensitivity Tests , Streptococcus oralis , Streptococcus mitis , Streptococcus salivarius , Antifungal Agents
Braz. oral res. (Online) ; 33: e023, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001611


Abstract: We evaluated the antifungal and antibiofilm potential of the hydroalcoholic extract of bark from Anadenanthera colubrina (vell.) Brenan, known as Angico, against Candida spp. Antifungal activity was evaluated using the microdilution technique through the Minimum Inhibitory and Fungicide Concentrations (MIC and MFC). The antibiofilm potential was tested in mature biofilms formed by Candida species and analyzed through the counting of CFU/mL and scanning electron micrograph (SEM). In vivo toxicity and therapeutic action was evaluated in the Galleria mellonella model. The treatment with the extract, in low doses, was able to reduce the growth of planktonic cells of Candida species. MIC values range between 19.5 and 39 µg/mL and MFC values range between 79 and 625 µg/mL. In addition was able to reduce the number of CFU/mL in biofilms and to cause structural alteration and cellular destruction, observed via SEM. A. colubrina showed low toxicity in the in vivo assay, having not affected the viability of the larvae at doses below 100mg/kg and high potential in the treatment of C. albicans infection. Considering its high antifungal potential, its low toxicity and potential to treatment of infections in in vivo model, A. colubrina extract is a strong candidate for development of a new agent for the treatment of oral candidiasis.

Candida/drug effects , Plant Extracts/pharmacology , Biofilms/drug effects , Fabaceae/chemistry , Antifungal Agents/pharmacology , Time Factors , Microscopy, Electron, Scanning , Colony Count, Microbial , Microbial Sensitivity Tests , Nystatin/pharmacology , Reproducibility of Results , Analysis of Variance
Rev. odontol. UNESP (Online) ; 48: e20190005, 2019. tab
Article in English | LILACS, BBO | ID: biblio-1043177


Abstract Introduction Actinic cheilitis is a inflammatory condition affecting mainly the lower lip and it is caused by chronic and excessive exposure of the lips to the ultraviolet radiation in sunlight. Objective Identifying clinical and histopathologic characteristics in 40 cases histopathologically diagnosed as actinic cheilitis. In addition, to investigate possible associations between these aspects. Method Defined as an observational, transversal, retrospective and descriptive study, it registered data regarding age, gender, occupation, symptomatology, records of sun exposure, frequency of sunblock use, tabagism, skin color, clinical aspect and histopathological classification. The data was submitted to the chi square test of Pearson (p<0.05). Result There was a predominance of male gender, leucodermia, and ages ranging between 50 and 60 years. The most common occupation was farming. From our sample, 85% had history of chronic sun exposure, in which 50% reported the use of some type of sunblock and only 25% were smokers. The main clinical condition was non-ulcerated leukoplakia and in the histopathological study, the hyperkeratosis were more common. A correlation between the degree of tissue alteration verified in the histopathological diagnosis and the studied clinical variables was not established (p=0.112). Conclusion The clinical aspect of the wound can conceal tissue alterations in different stages, emphasizing the importance of a premature diagnosis.

Resumo Introdução A queilite actínica e uma condição de natureza inflamatória que acomete o lábio inferior, e é causada pela exposição prolongada e crônica dos lábios à radiação ultravioleta proveniente dos raios solares. Objetivo Identificar as características clínicas e histopatológicas em uma série de 40 casos diagnosticados histopatologicamente como queilite actínica. Além disso, investigar possíveis associações entre estes aspectos. Método Caracterizado como um estudo observacional, transversal, retrospectivo e descritivo. Foram registrados dados a respeito da idade, gênero, ocupação, sintomatologia, histórico de exposição ao sol, uso de proteção solar, tabagismo, cor da pele, aspecto clínico e classificação histopatológica. Os dados foram submetidos ao teste de Qui-Quadrado de Pearson (p<0,05). Resultado Houve uma prevalência do gênero masculino, leucodermas, com faixa etária entre 50 e 60 anos e a ocupação mais presente foi a de agricultor. Da amostra, 85% apresentou histórico de exposição crônica ao sol, onde 50% relatou uso de algum tipo de proteção solar e apenas 25% era tabagista. A principal apresentação clínica foi leucoplasia não ulcerada, e no estudo histopatológico as hiperceratoses foram as mais presentes. Não foi possível correlacionar o grau de alteração tecidual verificada no diagnóstico histopatológico com as variáveis clínicas estudadas (p=0,112). Conclusão Não foi possível correlacionar o grau de alteração tecidual verificada no diagnóstico histopatológico com os aspectos clínicos observados. O aspecto clínico da lesão pode mascarar alterações teciduais em diversos estágios, o que enaltece a importância do diagnóstico precoce.

Humans , Male , Female , Adult , Middle Aged , Aged , Precancerous Conditions/pathology , Cheilitis , Clinical Study , Pathology, Clinical , Clinical Diagnosis
Rev. ABENO ; 19(1): 97-105, 2019. ilus, tab
Article in Portuguese | LILACS, BBO | ID: biblio-1022680


O termo currículo refere-se ao percurso seguido pelo estudante em uma instituição de ensino superior desde o momento em que inicia seu processo de formação profissional até a graduação. As Diretrizes Curriculares Nacionais (DCN) para os cursos de graduação em Odontologia, instituídas pela Resolução CNE/CES 3, de 19 de fevereiro de 2002, definem os princípios, fundamentos, condições e procedimentos da formação de cirurgiões-dentistas, primando pela formação de um profissional integral. As Instituições de Ensino Superior (IES) têm em mãos o desafio de construir projetos pedagógicos que operacionalizem esse conceito, de modo a atender as demandas da sociedade. Neste contexto, este estudo de abordagem qualitativa e exploratória objetivou analisar criticamente as matrizes curriculares de dois cursos de Odontologia, pertencentes a IES localizadas em regiões distintas do país. A metodologia utilizada considerou um modelo lógico para avaliação da formação do cirurgião-dentista com base nas DCN, proposto por Pessoa e Noro. Foram analisadas as dimensões referentes à integração entre os ciclos básico e profissionalizante, ao desenvolvimento de competências e habilidades desejáveis, a formação profissional, além do desenvolvimento do estágio curricular e atividades complementares. Foi verificado que as duas estruturas curriculares estão em consonância com os ideais preconizados pelas DCN, apesar de conterem pontos que não fortalecem a formação profissional do egresso. Observou-se que ambas as matrizes apresentam potencialidades e fragilidades, que inspiram discussões (AU).

The term "curriculum" refers to the program followed by students in an institution of higher learning from the moment they begin their course of professional training until graduation. The National Curricular Guidelines (NCG) for undergraduate studies in dentistry, instituted by Resolution CNE/CES 3, February 19, 2002, define the principles, basis, conditions and procedures for the training of fulltime professional dental surgeons. Higher Education Institutions (HEIs) face the challenge of designing pedagogical projects that implement these concepts in order to meet the demands of society. In this context, the objective of this qualitative and exploratory study was to critically analyze the curricular matrices of two dentistry programs from HEIs located in distinct regions of Brazil. A logical model based on national curriculum guidelines (NCG) proposed by Pessoa and Noro was used to evaluate the formation of dental surgeons. This study analyzed the integration between basic and professional activities, the development of desirable competencies and abilities, and professional development beyond residency and complementary activities. It verified that the curriculum structures of the two institutions are in agreement with the ideals advocated by the NCG, in spite of containing aspects which do not strengthen the profession formation of graduates. It was observed that both models have strengths and weaknesses, which should elicit further discussions (AU).

Competency-Based Education/methods , Curriculum/standards , Dental Staff , Education, Dental , Brazil , Qualitative Research , Health Human Resource Training
Braz. oral res. (Online) ; 32: e41, 2018. tab, graf
Article in English | LILACS | ID: biblio-889473


Abstract: The aim of this study was to evaluate in vitro the antifungal, antibiofilm and antiproliferative activities of the extract from the leaves of Guapira graciliflora Mart. The phytochemical characterization of the extract was performed using electrospray ionization mass spectrometry (ESI-MS). The antimicrobial activity of the extract and its fractions was evaluated using the broth microdilution method against species of Candida. The inhibition of C. albicans biofilm was evaluated based on the number of colony-forming units (CFU) and metabolic activity (MTT). The antiproliferative activity of the extract and its fraction was evaluated in the presence of human tumor and non-tumor cells, and the cytotoxicity of the extract was determined on the RAW 264.7 macrophage line - both using the sulforhodamine B method. The phytochemical characterization indicated the presence of the flavonoids rutin and kaempferol. The extract and the methanol fraction exhibited moderate antifungal activity against C. albicans, C. krusei, and C. glabrata, and strong activity against C. dubliniensis. In the biofilms at 24 and 48 hours, the concentration of 12500 µg/mL of the extract was the most effective at reducing the number of CFU s/mL (44.4% and 42.9%, respectively) and the metabolic activity of C. albicans cells (34.6% and 52%, respectively). The extract and its fractions had no antiproliferative effect on the tumor lines tested, with mean activity (log GI50) equal to or greater than 1.71 µg/mL. Macrophage cell viability remained higher than 80% for concentrations of the extract of up to 62.5 µg/mL. G. graciliflora has flavonoids in its chemical composition and demonstrates potential antifungal and antibiofilm activity, with no evidence of a significant change in the viability of human tumor and non-tumor cell lines.

Candida/drug effects , Plant Extracts/pharmacology , Biofilms/drug effects , Nyctaginaceae/chemistry , Cell Proliferation/drug effects , Antifungal Agents/pharmacology , Microbial Sensitivity Tests , Cell Survival/drug effects , Biofilms/growth & development , Lethal Dose 50 , Antifungal Agents/isolation & purification
ROBRAC ; 26(79): 9-15, out./dez. 2017. tab
Article in English | LILACS-Express | LILACS | ID: biblio-906015


Purpose: The aim of the present case-control study was to investigate whether there was any association between occupation and oral squamous cell carcinoma (OSCC) in a Brazilian population. Methods: This population-based study investigated an occupation that was classified according to the International Standard Classification of Occupations. The sample consisted of 665 individuals, of whom 133 were cases of OSCC, selected from reference hospitals for cancer in Paraíba; and 532 were part of a control group, paired by age, gender, place and smoking habit, who participated in the study. Results: There was statistically significant association between OSCC and occupation (p<0.001), alcohol consumption (p<0.001) and marital status (p=0.003). The variables marital status, occupation, alcoholism and smoking were shown to be statistically associated with the development of cancer of the oropharynx with Odds Ratio (OR) ranging from 1.8 to 11.2 Conclusion: Occupational exposure to chemical and physical carcinogenic substances, as well as the condition of living with a partner may be raised as possible risk factors for the development of cancer of the mouth and oropharynx.

Objetivo: O presente estudo de caso-controle teve como objetivo investigar se existe associação entre ocupação e carcinoma de células escamosas orais (OSCC) em uma população brasileira. Métodos: Este estudo populacional investigou a ocupação que foi classificada de acordo com a Norma Internacional de Classificação de Ocupações. Foram incluídos 665 indivíduos, sendo 133 casos de OSCC, selecionados de hospitais de referência para câncer na Paraíba e 532 participantes do grupo controle, pareados por idade, sexo, local e tabagismo. Resultados: Houve associação estatisticamente significativa entre ocupação (p<0,001), consumo de álcool (p<0,001) e estado civil (p=0,003). As variáveis estado civil, ocupação, alcoolismo e tabagismo mostraram-se estatisticamente associadas ao desenvolvimento de câncer da orofaringe com Odds Ratio (OR) entre 1,8 e 11,2. Conclusão: A exposição ocupacional às substâncias químicas e físicas cancerígenas, bem como a condição de viver com um parceiro podem aumentar a possibilidade de fatores de risco para o desenvolvimento de câncer de boca e orofaringe.

RGO (Porto Alegre) ; 65(4): 376-379, Oct.-Dec. 2017. graf
Article in English | LILACS, BBO | ID: biblio-896046


ABSTRACT Orthokeratinized odontogenic cysts are developmental odontogenic cysts, presenting with low frequency, low rate of recurrence and their ethiopathogenesis is unknown. Radiographically, they show a radiolucent area in the mandibular posterior region. The aim of this report is to describe an unusual case of orthokeratinized odontogenic cysts, radiographically exhibiting radiopaque areas with an aspect of calcifications in the lesion. Based on this, the clinical hypothesis of ameloblastic fibro-odontoma was suggested. After incisional biopsy and microscopic analysis, the conclusive diagnosis was orthokeratinized odontogenic cysts. The radiopaque foci were observed to be associated with a foreign body reaction. The patient was submitted to surgery under local anesthesia, with intraoral access for complete excision of the lesion and to re-establish esthetics. After follow-up of 24 months there were no signs of recurrence. Knowledge of this type of reaction is important because of the risk that the lesion may mimic a potentially more aggressive lesion, affecting the choice of treatment.

RESUMO Os cistos odontogênicos ortoceratinizados são cistos de desenvolvimento de origem odontogênica, de baixa frequência, etiopatogenia desconhecida e baixa taxa de recorrência. Radiograficamente, apresenta-se como uma área radiolúcida em região posterior de mandíbula. O objetivo deste artigo é descrever um caso não usual de cistos odontogênicos ortoceratinizados, que exibia, radiograficamente, focos radiopacos semelhantes à calcificações, no interior da lesão, sugerindo diagnóstico clínico de fibro-odontoma ameloblástico. Após a biópsia incisional e análise microscópica, o diagnóstico conclusivo foi de cistos odontogênicos ortoceratinizados. Observou-se que os focos radiopacos estavam associados, microscopicamente, a presença de uma reação de células gigantes multinucleadas por corpo estranho. O paciente foi tratado cirurgicamente, sob anestesia local, com acesso intrabucal para remoção total da lesão, com o restabelecimento da estética e sem sinais de recidiva após 24 meses. A importância do conhecimento deste tipo de reação dar-se pelo risco de mimetizar uma lesão potencialmente mais agressiva, direcionando a escolha do tratamento da lesão.

Pesqui. bras. odontopediatria clín. integr ; 17(1): e3331, 13/01/2017. tab, ilus
Article in English | LILACS, BBO | ID: biblio-914241


Objective: To compare the rate of cell proliferation and expression of antiapoptotic protein Bcl-2 between drug-induced gingival overgrowth (DIGO) and clinical healthy gingiva (CHG) and to establish associations with histopathological features. Material and Methods: Twenty specimens of DIGO and 20 CHG specimens were submitted to morphological and immunohistochemical analysis by light microscopy. Cell proliferation (Ki-67) and the expression of Bcl-2 were evaluated in epithelial cells and spindle-shaped mononuclear cells of the connective tissue by establishing the labeling index (LI). Results: In epithelial tissue, the mean LI for Ki-67 was 17.2% in DIGO and 21.71% in CHG (p = 0.137). The mean LIs for Bcl-2 in epithelial tissue were 14.67% and 10.24% in DIGO and CHG, respectively (p = 0.026). In connective tissue, DIGO and CHG specimens exhibited low LIs for Ki-67 and Bcl-2, with mean values of less than 0.5% in both groups. No significant differences in the LIs for Ki-67 or Bcl-2 in epithelial tissue were observed according to the degree of collagenization, degree of vascularization and intensity of inflammatory infiltration (p > 0.05). No significant correlations were observed between the LIs for Ki-67 and Bcl-2 (p > 0.05). Conclusion: The present results suggest that the pathogenesis of DIGO does not involve increased proliferation or decreased apoptosis of fibroblasts. On the other hand, the morphological pattern of elongated epithelial cristae observed in DIGO could mainly be due to the inhibition of keratinocyte apoptosis and not to increased proliferation of these cells.

Cell Proliferation , Fibromatosis, Gingival/pathology , Genes, bcl-2 , Immunohistochemistry/methods , Ki-67 Antigen , Brazil , Statistics, Nonparametric
Pesqui. bras. odontopediatria clín. integr ; 17(1): e3389, 13/01/2017. tab, ilus, graf
Article in English | LILACS, BBO | ID: biblio-914260


Objective: To investigate the antifungal potential of A. colubrina, and its phytochemical characteristics, thermal profile and toxicity. Material and Methods: To assess potential antifungal activity, the technique of microdilution was used with the determination of the Minimum Inhibitory Concentration and Minimum Fungicidal Concentration, using standard species of Candida and recent clinical isolates of Candida albicans. Analyses of action of the extract were performed on the wall and cell morphology of C. albicans, of the interactive effect between the plant extract and nystatin on C. albicans through the checkerboard method, and of growth kinetics. The phytochemical screening was determined by spectrophotometry. The thermal profile was traced with the determination of thermogravimetric curves (TG) and differential scanning calorimetry (DSC). The toxicity was evaluated by the method of hemolysis. Results: The extract of A. colubrina showed a fungistatic potential against all bacteria tested and it acted by modifying the cellular morphology of C. albicans. There was a synergism between nystatin and the plant extract (FIC=0.375), and 53.18% of total polyphenols were determined. The TG curve showed the occurrence of three steps of thermal decomposition. None of the tested concentrations became the effective cytotoxic concentration. Conclusion: Further studies should be conducted to understand the efficacy and the mechanisms of action involved in the antifungal activity of the plant extract of A. colubrina in order to produce a new drug for the treatment of oral candidiasis.

Antifungal Agents , Candida albicans/immunology , Plant Extracts , Plants, Medicinal , Anti-Infective Agents , Brazil , Spectrometry, Fluorescence/methods
Pesqui. bras. odontopediatria clín. integr ; 17(1): e3732, 13/01/2017. tab
Article in English | LILACS, BBO | ID: biblio-914466


Objective: To investigate if an association exists between diet and oral squamous cell carcinoma (OSCC) in a Brazilian population. Material and Methods: This populationbased study investigated food groups intaken by means of a quantitative food frequency questionnaire (QFFQ) validated for use in Brazil. It was included 665 individuals, being 133 cases of OSCC, selected from reference hospitals for cancer in Paraíba and 532 being part of a control group, paired by age, gender, place and smoking habit took part in the study. Food consumption related to cases and control groups were evaluated by QFFQ. The Chi-square test was carried out in order to verify if there was association between the categorical variables. The level of significance was 5%. Conditional logistic regression was performed by the Enter method in order to verify the odds ratio of independent variables that predict OSCC. Results: It was found statistical association between OSCC and: processed and variety meats (p=0.048), dairy products (p<0.001), oils and fats (p<0.001) and alcoholic beverages (p<0.001). The high consumption of cereals and tubers (OR=0.53; CI95%: 0.29-0.96; p=0.0039) acted as protection factor for OSCC. Conclusion: Data of the present research suggest that the ingestion of animal fat, food rich in salt and refined carbohydrates were associated with OSCC cases.

Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Carcinoma, Squamous Cell/pathology , Case-Control Studies , Diet/methods , Mouth Diseases/pathology , Brazil , Chi-Square Distribution , Eating , Logistic Models , Surveys and Questionnaires
An. bras. dermatol ; 91(4): 463-467, July-Aug. 2016. tab, graf
Article in English | LILACS | ID: lil-792441


Abstract: Background: The studies found in the literature associate the immunoexpression of hMLH1 and hMSH2 proteins with histologic aspects, but do not correlate it with clinical and epidemiological data. Objective: To evaluate the immunoexpression of hMLH1 and hMSH2 in actinic cheilitis, correlating it with clinical characteristics. Methods: We analyzed 40 cases. Histological and immunohistochemical analyses were performed. The following clinical variables were evaluated: gender, age range, ethnicity, clinical aspect and occupational sunlight exposure. Statistical evaluation included the Student t-test, while the significance level was set at 5%. Results: Greater immunoexpression of hMLH1 and hMSH2 was observed in females, individuals aged over 40, and mixed-race/black patients. Furthermore, the immunoexpression of these proteins was greater in actinic cheilitis with a white-colored appearance and in patients without occupational sunlight exposure. No statistical differences were observed for the variables studied. Conclusion: This study uncovered variations of hMLH1 and hMSH2 protein expression upon evaluation of clinical aspects in actinic cheilitis.

Humans , Male , Female , Adult , Middle Aged , Aged , Cheilitis/metabolism , MutS Homolog 2 Protein/analysis , MutL Protein Homolog 1/analysis , Precancerous Conditions/metabolism , Reference Values , Skin/metabolism , Severity of Illness Index , Immunohistochemistry , Sex Factors , Risk Factors , Age Factors , MutS Homolog 2 Protein/metabolism , MutL Protein Homolog 1/metabolism
Pesqui. bras. odontopediatria clín. integr ; 16(1): 177-184, jan.-dez. 2016. tab
Article in English | LILACS, BBO | ID: biblio-911340


Objective: To evaluate the knowledge level of dentists about oral cancer (OC) in Campina Grande, Paraiba, Brazil. Material and Methods: It was an observational, transversal, descriptive and analytical study. It was applied 200 structured forms. The sample was chosen for convenience. Statistical associations were performed using the Chi-square and Fisher's exact tests (P<0.05). Results: Of the 200 interviewed dentists, 66% (n=132) were women, the prevalent age group was 21-40 years (49.5%), 47% work at the private sector and 39% concluded their graduation 20 years ago. Oral squamous cell carcinoma was cited as the most common histopathologic type of OC (65.5%), and the most common anatomical site of OC was tongue (53.4%). The main risk factors cited for the developing of OC were licit and/or illicit drugs (99%), heredity (75.9%), dental problems (74.9%) and sun exposure (74.4%). There was a significant statistically association between the variables tobacco and/or alcohol use and gender of the dentists (P=0.001), between the knowledge level about OC and its histopathologic type and the more affected anatomical site (p=0.012 and p=0.034, respectively). Conclusion: The level of knowledge of dentists about OC is still insufficient for the performance of early diagnosis. Therefore, further discussions about this theme in dentistry schools should be conducted in order to train qualified professionals for this type of diagnosis.

Humans , Male , Female , Adult , Brazil , Dentists , Health Knowledge, Attitudes, Practice , Mouth Neoplasms , Chi-Square Distribution , Observational Study
Braz. oral res. (Online) ; 30(1): e95, 2016. tab, graf
Article in English | LILACS | ID: biblio-952056


Abstract The objective of this study was to analyze the presence of tumor-associated macrophage (TAM) subpopulations M1 and M2 in squamous cell carcinoma of the lower lip (SCCLL) by immunohistochemitry, and to evaluate the possible role of these subtypes in the development of regional lymph node metastasis and their association with clinical and pathological parameters. Forty-two cases of SCCLL were divided into two groups (21 with and 21 without regional lymph node metastasis). The histopathological grade of malignancy was determined and the material was submitted to double staining with anti-CD68/anti-CD163 and anti-CD68/anti-HLA-DR monoclonal antibodies. The results were analyzed statistically using the Wilcoxon signed-rank and Spearman correlation tests. The M1 and M2 subpopulations were observed in all cases studied. No significant difference was observed between the quantities of M1 and M2 TAMs regarding tumor size (p > 0.05). A significantly larger number of M2 compared to M1 TAMs was observed in tumors without regional lymph node metastasis, tumors in early stages, and low-grade tumors (p < 0.05). No significant difference between the numbers of M1 and M2 TAMs was observed in tumors with regional lymph node metastasis, tumors in advanced stages, and high-grade tumors (p > 0.05). There was a positive weak correlation between M1 and M2 TAMs (r = 0.361; p = 0.019). The results suggest a more important role of M2 TAMs in early stages than advanced stages of lip carcinogenesis. The progression of SCCLL does not seem to be related to an imbalance of macrophage polarization in the microenvironment of these tumors.

Humans , Male , Female , Lip Neoplasms/pathology , Carcinoma, Squamous Cell/pathology , Macrophages/pathology , Reference Values , Immunohistochemistry , Antigens, Differentiation, Myelomonocytic , HLA-DR Antigens , Antigens, CD , Cell Count , Retrospective Studies , Paraffin Embedding , Receptors, Cell Surface , Statistics, Nonparametric , Neoplasm Grading , Carcinogenesis , Lymphatic Metastasis , Neoplasm Staging
Braz. oral res. (Online) ; 30(1): e130, 2016. tab, graf
Article in English | LILACS | ID: biblio-952013


Abstract: The aim of this study was to determine the number of FoxP3+ regulatory T (Treg) cells in the microenvironment of lower lip squamous cell carcinomas (LLSCCs) and to correlate the findings with clinicopathological parameters (tumor size/extent, regional lymph node metastasis, clinical stage, and histopathological grade of malignancy). Fifty cases of LLSCC were selected. Lymphocytes exhibiting nuclear immunostaining for FoxP3 were quantified in 10 microscopic fields at the deep invasive front of LLSCCs. The results were analyzed statistically using the nonparametric Mann-Whitney test and Fisher's exact test. FoxP3+ lymphocytes were observed in all cases studied. The number of these cells tended to be higher in smaller tumors, tumors without regional lymph node metastasis, and tumors in early clinical stages, but the difference was not statistically significant (p > 0.05). Low-grade tumors contained a larger number of FoxP3+ lymphocytes than high-grade tumors (p = 0.019). Tumors with an intense inflammatory infiltrate exhibited a larger number of Treg cells (p = 0.035). On the other hand, the number of FoxP3+ lymphocytes was smaller in tumors arranged in small cell clusters (p = 0.003). No significant differences in the number of FoxP3+ lymphocytes were observed according to the degree of keratinization (p = 0.525) or nuclear pleomorphism (p = 0.343). The results suggest the participation of Treg cells in immune and inflammatory responses in the microenvironment of LLSCCs. These cells may play a more important role in early stages rather than in advanced stages of lip carcinogenesis.

Humans , Male , Female , Adult , Aged , Aged, 80 and over , Carcinoma, Squamous Cell/pathology , T-Lymphocytes, Regulatory/chemistry , Forkhead Transcription Factors/analysis , Reference Values , Lip Neoplasms/pathology , Immunohistochemistry , Cell Count , T-Lymphocytes, Regulatory/pathology , Tumor Burden , Tumor Microenvironment , Neoplasm Grading , Lymphatic Metastasis , Middle Aged , Neoplasm Staging
An. bras. dermatol ; 90(1): 49-53, Jan-Feb/2015. tab, graf
Article in English | LILACS | ID: lil-735730


BACKGROUND: Patients with diabetes mellitus have been associated with a number of changes in the oral cavity, such as gingivitis, periodontitis, mucosal diseases, salivary dysfunction, altered taste, and burning mouth. OBJECTIVES: To determine the prevalence of oral mucosal lesions in patients with diabetes mellitus. METHODS: A cross-sectional observational study between August and October 2012 with a convenience sampling was performed for 51 patients with diabetes mellitus (type 1 and type 2). The study consisted of two phases: 1) a questionnaire application; 2) intraoral clinical examination. For the analysis of data, we used descriptive statistics, Fisher's exact test in bivariate analysis (significance level of 0.05), and Poisson Regression. RESULTS: The prevalence of oral lesions was 78.4%. Traumatic ulcers (16.4%) and actinic cheilitis (12.7%) were the most prevalent lesions. The lips (35.3%) and tongue (23.5%) were the most common location. The bivariate analysis showed an association with the type of diabetes, and two variables (age and comorbidity) were quite close to the significance level. In the Poisson Regression analysis, only diabetes type 2 remained significant after adjusting the model. CONCLUSIONS: The results of this study show a high prevalence of oral mucosal lesions in diabetic patients. The oral mucosal lesions are mostly associated with diabetes type 2. .

Aged , Female , Humans , Male , Middle Aged , Diabetes Mellitus, Type 1/complications , /complications , Mouth Mucosa , Mouth Diseases/etiology , Brazil/epidemiology , Cross-Sectional Studies , Diabetes Mellitus, Type 1/epidemiology , /epidemiology , Mouth Diseases/epidemiology , Mouth Diseases/pathology , Mouth Mucosa/pathology , Oral Health , Poisson Distribution , Prevalence , Sex Distribution , Socioeconomic Factors
Rev. Assoc. Paul. Cir. Dent ; 69(2): 165-168, 2015. ilus
Article in Portuguese | LILACS, BBO | ID: lil-770818


Plasmocitoma é uma proliferação clonal e neoplásica de células plasmáticas. Paciente, sexo feminino, 51 anos, sem história de tabagismo e consumo de álcool mostrou leve inflamação e inchaço na gengiva vestibular do dente 48 com mobilidade grau I. Radiograficamente verificou--se a presença de imagem radiolúcida multilocular na região do ângulo mandibular. Durante a cirurgia confirmou-se a presença de uma lesão destacável, friável, contendo líquido acastanhado e purulento em seu interior. A lesão foi totalmente removida em conjunto com o dente 48. Ao exame histopatológico (HE), foi identificada uma neoplasia maligna caracterizada pela proliferação de células plasmocitoides pleomórficas com núcleos intensamente hipercromáticos. Estas células exibiam núcleo basofílico com notável distribuição irregular da cromatina. Para confirmar o diagnóstico procedeu a avaliação imuno-histoquímica, que mostrou positividade para Plasm cell e Lambda e negatividade para Kappa. Com base nestes resultados concluiu-se o diagnóstico de plasmocitoma solitário do osso.

Plasmacytoma is a clonal proliferation and neoplastic of plasma cells. Patient, female, 51 years old, no history of smoking and alcohol consumption showed mild inflammation and swelling in the oral gingiva of the tooth 48 with mobility grade I. Radiographically verified the presence of radiolucent and multilocular image in the region of the mandibular angle. During surgery confirmed the presence of a lesion detachable, friable, browned and containing purulent fluid inside. This lesion was removed fully together with the tooth 48. Histopathology (HE) was identified a malignant neoplasm characterized by proliferation of plasmacytoid cells intensely hyperchromatic and pleomorphic. These cells exhibited large basophilic nucleus with remarkable irregular chromatin distribution. To confirm the diagnosis proceeded immunohistochemistry evaluation which showed positivity for Plasma Cell and Lambda and negativity of Kappa. Based on these findings concluded the diagnosis of plasmacytoma.

Humans , Female , Adult , Diagnosis , Bone and Bones/physiology , Bone and Bones/injuries , Plasmacytoma/complications , Plasmacytoma/diagnosis
RGO (Porto Alegre) ; 62(4): 425-430, Oct-Dec/2014. graf
Article in English | LILACS-Express | LILACS | ID: lil-741676


Lipoma is a benign mesenchymal neoplasm of soft tissue composed of mature adipocyte cells, being among the most common benign tumors. However, it has been reported that they are uncommon in the oral cavity, representing between 15% and 20% of cases involving the head and neck, and between 1% and 5% of all benign neoplasms of the oral cavity. As for their clinical features, lipomas present as slow-growing and asymptomatic, with an associated yellowish submucosal growth, and may not be well circumscribed. Lipomas are known to grow to around 0.5 to 2 cm, but can have very large maximum diameters. Surgical excision is the treatment of choice, recurrence being rare. The reported case is of a 74 year old, female patient, exhibiting a large lipoma in the right genial-masseteric region, which is unusual with the clinic presentation of lipomas occurring on the face. The patient was subjected to the complete removal of the lesion, was monitored for 6 months and presented no signs of recurrence.

Lipoma é uma neoplasia mesenquimal benigna de tecido mole constituída de células adiposas maduras, estando no grupo dos tumores benignos mais comuns. No entanto, é relatada como incomum na cavidade oral, representando de 15 a 20% dos casos envolvendo a região de cabeça e pescoço, e de 1 a 5% de todos os neoplasmas benignos da cavidade oral. Quanto as suas características clínicas, apresentam um crescimento lento, indolor, associado a uma massa submucosa amarelada, podendo não se apresentarem bem delimitados. Os lipomas pelo seu crescimento médio de 0,5 a 2cm, podem atingir tamanhos exuberantes em seu maior diâmetro. A excisão cirúrgica é o tratamento de escolha, sendo raras as recidivas. O presente relato apresentado refere-se a uma paciente de 74 anos de idade, sexo feminino, apresentando um lipoma de grande extensão em região geniana-massetérica direita, não usual com a apresentação clínica dos lipomas que ocorrem na face. A paciente foi submetida à remoção completa da lesão, sendo acompanhada por 6 meses, sem sinais de recidiva.