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1.
J. appl. oral sci ; 30: e20210575, 2022. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1365010

ABSTRACT

Abstract Objective: To assess the effects of different peracetic acid (PAA) formulations on smear layer (SL) removal, dentine erosion, cytotoxicity, and antibiofilm activity. Methodology: SL removal and dentine erosion were assessed using 90 premolars, distributed into six groups, according to final irrigation: PAA formulations (1% Sigma, 1% Bacterend OX, 1% Arposept, and 0.09-0.15% Anioxyde), 17% ethylenediaminetetraacetic acid (EDTA), and water (control). Cytotoxicity was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red assays. Antibacterial and antibiofilm effectiveness was evaluated against Enterococcus faecalis. For cytotoxicity and antibiofilm activity assessment, the 2.5% NaOCl was also included. Results: EDTA, Sigma, and Bacterend OX removed more SL than Arposept, Anioxyde, and water (p<0.05). EDTA caused more severe dentine erosion than Sigma and Bacterend OX (p<0.05). Sigma and Bacterend OX had higher cytotoxicity than the other solutions (p<0.05). NaOCl, Bacterend OX, Sigma, and Anioxyde significantly reduced E. faecalis colony-forming units (CFU) (p<0.05). The 2.5% NaOCl solution promoted greater biofilm biomass reduction (p<0.05) than the other solutions. All PAA formulations promoted greater biomass reduction than 17% EDTA (p<0.05). Conclusions: Although Sigma and Bacterend OX had higher cytotoxicity, they had a SL removal capability similar to that of EDTA, were as effective as NaOCl against E. faecalis biofilm, and promoted less dentine erosion than EDTA. Arposept and Anioxyde failed to remove the SL, had lower cytotoxicity, and showed less bacterial activity than NaOCl.

2.
Braz. dent. j ; 32(4): 8-18, July-Aug. 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1345521

ABSTRACT

Abstract The aim of this study was to evaluate the physicochemical properties, cytocompatibility and antibiofilm activity of a new calcium silicate-based endodontic sealer, Sealer Plus BC (MK Life, Brazil), in comparison with TotalFill BC Sealer (FKG Dentaire SA, Switzerland) and AH Plus (Dentsply, Germany). Setting time and flow were evaluated based on ISO 6876 standard. The pH was evaluated after different periods, and radiopacity by radiographic analysis (mmAl). Solubility (% mass loss) and volumetric change (by micro-CT) were assessed after 30 days of immersion in distilled water. Cytocompatibility was assessed by methyltetrazolium (MTT) and neutral red (NR) assays, after exposure of Saos-2 cells to the sealer extract for 24 h. An additional analysis was performed by using MTT assay after 1, 3 and 7 days of exposure of Saos-2 to the sealers 1:8 dilution extracts. Antibiofilm activity against Enterococcus faecalis and/or Candida albicans was evaluated by crystal violet assay and modified direct contact test. The physicochemical properties were analyzed using ANOVA/Tukey tests; MTT and NR data were analyzed by ANOVA and Bonferroni tests; the antimicrobial tests were analyzed by Kruskal-Wallis and Dunn tests (α=0.05). Sealer Plus BC had proper setting time, radiopacity, flow and alkalization capacity. Sealer Plus BC was significantly more soluble than AH Plus (p<0.05) and presented volumetric change similar to AH Plus and TotalFill BC (p>0.05). Sealer Plus BC presented antibiofilm activity and no cytotoxic effect. In conclusion, although Sealer Plus BC had higher solubility, this sealer showed proper physicochemical properties, cytocompatibility, and antibiofilm activity.


Resumo O objetivo deste estudo foi avaliar as propriedades físico-químicas, a citocompatibilidade e a atividade antibiofilme de um novo cimento endodôntico à base de silicato de cálcio, Sealer Plus BC (MK Life, Brasil), em comparação com TotalFill BC Sealer (FKG Dentaire SA, Suíça) e AH Plus (Dentsply, Alemanha). O tempo de presa e o escoamento foram avaliados com base nas normas ISO 6876. O pH foi avaliado após diferentes períodos, e a radiopacidade por análise radiográfica (mmAl). A solubilidade (% de perda de massa) e alteração volumétrica (por micro-CT) foram avaliadas após 30 dias de imersão em água destilada. Citocompatibilidade foi avaliada pelos ensaios metiltetrazólio (MTT) e vermelho neutro (NR), após exposição das células Saos-2 ao extrato de cimento por 24 horas. Análise adicional foi realizada através do ensaio MTT após 1, 3 e 7 dias de exposição das células Saos-2 aos extratos dos cimentos na diluição de 1:8. Atividade antibiofilme contra Enterococcus faecalis e/ou Candida albicans foi avaliada pelos ensaios cristal violeta e contato direto modificado. As propriedades físico-químicas foram analisadas utilizando os testes ANOVA e Tukey; MTT e NR foram analisados pelos testes ANOVA e Bonferroni; os ensaios antimicrobianos foram analisados pelos testes Kruskal-Wallis e Dunn (α=0.05). Sealer Plus BC apresentou tempo de presa, radiopacidade e escoamento adequados, além de capacidade de alcalinização. Sealer Plus BC foi significantemente mais solúvel que AH Plus (p<0.05) e apresentou alteração volumétrica similar à de AH Plus e TotalFill BC (p>0.05). Sealer Plus BC apresentou atividade antibiofilme, sem efeito citotóxico. Como conclusão, embora Sealer Plus BC apresente maior solubilidade, este cimento apresentou propriedades físico-químicas adequadas, citocompatibilidade e atividade antibiofilme.


Subject(s)
Root Canal Filling Materials/pharmacology , Materials Testing , Silicates/pharmacology , Calcium Compounds/pharmacology , Biofilms , Epoxy Resins
3.
Dent. press endod ; 11(1): 72-77, Jan-Apr2021. Tab, Ilus
Article in English | LILACS | ID: biblio-1348251

ABSTRACT

Introdução: O tipo de agulha e fluxo de irrigação é importante para a limpeza adequada no terço apical do canal radicular. Objetivo: Avaliar a influência do tipo de agulha e fluxo de irrigação na limpeza do canal radicular e canais laterais simulados e na extrusão apical do irrigante. Métodos: Trinta e dois dentes de resina foram utiliza- dos. Após a instrumentação do canal radicular, foram feitos quatro canais laterais a 2 e 7 mm do ápice. Os canais radiculares foram preenchidos com solução de contraste. Os canais foram irrigados com dois tipos de agulha, 29G e 31G, com diferentes designs (abertura lateral e apical) e dois fluxos (2 ou 5 mL/min), a 1 mm aquém do comprimento de trabalho. O volume da solução de contraste nos canais principal e laterais após irrigação e a extrusão apical do irrigante (mm3 ) foi avaliado por micro-CT, em comparação com a análise inicial. Os dados em porcentagem foram analisados pelos testes ANOVA e Tukey (α=0,05). Resultados: não houve diferença entre os protocolos de irrigação para limpeza da solução de contraste. Maior volume de extrusão apical com agulha 29G com abertura apical e 5 mL/min foi observado, em comparação ao mesmo tipo de agulha e 2 mL/min (p<0,05). Conclusão: Todos os protocolos de irrigação foram associados à limpeza dos canais radiculares. Maior extrusão apical foi observada com agulha 29G ­ abertura apical com maior fluxo de irrigação (5 mL/min). Implicações clínicas: Irrigação endodôntica com agulha com abertura apical e maior fluxo da solução pode favorecer a extrusão dos irrigantes para os tecidos periapicais (AU).


Introduction: needle and irrigation flow rate are important for proper cleaning of the root canal. Aim: to evaluate the influence of type of needle and irrigation flow rate on cleaning of root canal and simulated lateral canals and the apical extrusion of irrigant. Methods: Thirty-two resin teeth were used. After root canal instrumentation, four lateral canals were made at 2 and 7 mm from the apex. Root canals were filled with contrast solution. The root canals were irrigated with two types of needle, 29G and 31G, with different designs (side and apical opening) and two flow rates (2 or 5 mL/min), at 1 mm short of the working length. The volume of the contrast solution in the main and lateral canals after irrigation and apical extrusion of the irrigant (mm3) were evaluated by Micro-CT, in comparison with the initial analysis. Data in percentage were analyzed by ANOVA and Tukeys tests (α = 0.05). Results: no difference among the irrigation protocols for contrast solution cleaning was observed. Higher volume of apical extrusion using needle 29G-apical opening and 5 mL/min in comparison with the same type of needle and 2 mL/min was observed (p < 0.05). Conclusion: all irrigation protocols were associated with root canals cleaning. Greater apical extrusion was observed for needle 29G-apical opening with higher irrigation flow rate (5 mL/min). Clinical implications: endodontic irrigation using a needle with apical opening and higher flow rate of solution may favor extrusion of irrigant to the periapical tissues (AU).


Subject(s)
Periapical Tissue , Root Canal Irrigants , X-Ray Microtomography , Needles , Dental Pulp Cavity , Housekeeping
4.
Braz. oral res. (Online) ; 35: e074, 2021. tab, graf
Article in English | LILACS-Express | LILACS, BBO | ID: biblio-1339459

ABSTRACT

Abstract The aim of this study was to assess the influence of micro-computed tomography (micro-CT) voxel size on dentinal microcrack detection after root canal preparation using rotary heat-treated nickel-titanium files. Curved mesial root canals (n = 24) of mandibular molars were prepared using ProDesign Logic 30/.05 (PDL) or HyFlex EDM 25/.08 (HEDM). The specimens were scanned by micro-CT at 5 μm voxel size before and after root canal preparation. The percentage of microcracks was evaluated in images at 5, 10 and 20 μm voxel size, by two examiners at two moments. The Kappa and McNemar tests (α = 0.05) were used. The percentage of dentinal microcracks was similar before and after PDL and HEDM preparations, at 10 and 20 μm (p > 0.05). HEDM showed a higher percentage of dentinal microcracks in the middle third at 5 μm after preparation (p < 0.05). The detection of dentinal microcracks before and after instrumentation using PDL was more accurate at 5 μm than at 20 μm, in all thirds (p < 0.05). Within the limitations of this ex vivo study, as expected, the results showed that different resolutions influence the micro-CT analysis of microcracks. The highest accuracy in detecting microcracks was observed for analyses performed at 5 μm voxel size. HyFlex EDM caused even more microcracks to develop in the middle third, detectable only by visualization of images made at 5 μm voxel size.

5.
J. appl. oral sci ; 29: e20200870, 2021. tab, graf
Article in English | LILACS | ID: biblio-1250190

ABSTRACT

Abstract Bio-C Pulpecto (Bio-CP) was recently developed as the first bioceramic root filling material for primary teeth. Objective To evaluate the physicochemical properties of radiopacity, setting time, pH, cytocompatibility and potential of Bio-CP to induce mineralisation, compared with (1) Calen thickened with zinc oxide (Calen-ZO), and (2) zinc oxide and eugenol (ZOE). Methodology Physicochemical properties were evaluated according to ISO 6876. Saos-2 (human osteoblast-like cell line) exposed to extracts of the materials were subjected to assays of methyl thiazolyl tetrazolium, neutral red, alkaline phosphatase (ALP) activity and mineralised nodule production. The results were analysed using one-way or two-way ANOVA and Tukey's or Bonferroni's post-tests (α=0.05). Results All the materials showed radiopacity higher than 3 mm Al. Bio-CP had lower pH than Calen-ZO, but higher pH than ZOE. Calen-ZO and Bio-CP did not set. The setting time for ZOE was 110 min. The cytocompatibility order was Calen-ZO > Bio-CP > ZOE (1:2, 1:4 dilutions) and Calen-ZO > Bio-CP = ZOE (1:12, 1:24 dilutions) and Calen-ZO = Bio-CP > ZOE (1:32 dilution). Bio-CP induced greater ALP activity at 7 days, and greater mineralised nodule production, compared to Calen-ZO (p<0.05). Conclusions Bio-CP showed adequate physicochemical properties, cytocompatibility and potential to induce mineralisation.


Subject(s)
Humans , Root Canal Filling Materials , Dental Pulp Cavity , Osteoblasts , Root Canal Obturation , Tooth, Deciduous , Zinc Oxide-Eugenol Cement , Biology
6.
Rev. odontol. UNESP (Online) ; 48: e20190112, 2019. tab, ilus
Article in English | LILACS, BBO | ID: biblio-1099186

ABSTRACT

Abstract Introduction Flow and filling ability of root canal sealers are indispensable for hermetic sealing of the root canal. Micro-computed tomography (micro-CT) can be used as a complementary methodology to evaluate such properties. Objective To evaluate the flow and filling ability of AH Plus, Endofill and Sealapex by conventional methodology and micro-CT. Material and method The flow of the sealers was analyzed according to ISO 6876/2012 and complemented by the area evaluation. Glass plates were manufactured with diameters of 1×1×2 mm and 1×1×1 mm (length, width and height), with a central cavity and four grooves in the horizontal and vertical directions. Each material was placed in the central cavity. Another glass plate and a metal weight were placed on the cement and kept for 10 minutes. The glass plate/sealer set was scanned using micro-CT. The flow was calculated by linear measurement of the material in the grooves. The central filling (mm3) was calculated in the central cavity and the lateral filling was measured up to 2 mm from the central cavity. Data were submitted to ANOVA/Tukey tests (α=0.05). Result All evaluated sealers presented flow according to ISO 6876 standards. The materials showed central cavity filling capacity higher than 80% and lateral filling greater than 75%. There was no difference in flow (mm and mm2) and in the filling ability (mm3) provided by the materials (p>0.05). Conclusion All evaluated root canal sealers showed adequate flow and filling capacity, suggesting their clinical application.


Resumo Introdução Escoamento e capacidade de preenchimento de cimentos obturadores são indispensáveis para um selamento hermético do canal radicular. Microtomografia computadorizada (micro-CT) pode ser utilizada como uma metodologia complementar para avaliação de tais propriedades. Objetivo Avaliar escoamento e capacidade de preenchimento de AH Plus, Endofill e Sealapex, por meio de metodologia convencional e micro-CT. Material e método O escoamento dos cimentos foi analisado de acordo com as normas ISO 6876/2012 e complementado pela avaliação em área. Placas de vidro foram confeccionadas nos diâmetros de 1×1×2 mm e 1×1×1 mm (comprimento, largura e altura), com uma cavidade central e quatro canaletas nas direções horizontal e vertical. Cada material foi colocado na cavidade central. Outra placa de vidro e um peso de metal foram colocados sobre o cimento e mantidos por 10 minutos. O conjunto placa de vidro/cimento foi escaneado usando micro-CT. O escoamento foi calculado por medição linear do material nas canaletas. O preenchimento (mm3) central foi calculado na cavidade central e o preenchimento lateral foi medido até 2 mm a partir da cavidade central. Os dados foram submetidos aos testes ANOVA/Tukey (α=0.05). Resultado Todos os cimentos avaliados apresentaram escoamento de acordo com as normas ISO 6876. Os materiais mostraram capacidade de preenchimento da cavidade central superior a 80% e preenchimento lateral superior a 75%. Não houve diferença no escoamento (mm e mm2) e na capacidade de preenchimento (mm3) proporcionada pelos materiais (p>0.05). Conclusão Todos os cimentos obturadores avaliados mostraram adequado escoamento e capacidade de preenchimento, sugerindo a aplicação clínica dos mesmos.


Subject(s)
Root Canal Filling Materials , Dental Cements/analysis , X-Ray Microtomography/methods , Root Canal Therapy
7.
J. appl. oral sci ; 27: e20180689, 2019. tab, graf
Article in English | LILACS, BBO | ID: biblio-1012523

ABSTRACT

Abstract Objective The aim of this study was to evaluate the root canal preparation and apical enlargement of molar root canals with rotary or reciprocating heat-treated nickel-titanium (NiTi) instruments, by using micro-computed tomography (micro-CT). Methodology Mesial root canals (n=48) of mandibular molars, with a curvature between 20° and 40°, were prepared with ProDesign Logic (PDL) 25.01 and 25.06 in rotary motion, or ProDesign R (PDR) 25.06 in reciprocating motion (PDR). Apical enlargement was performed with PDL35.01 and PDL35.05 or PDR35.05. Scanning with 9 µm resolution was performed before and after preparation, and, after apical enlargement, by using micro-CT. The percentage of volume increase, debris and untouched root canal surface, transportation, centralization and preparation time were analyzed. ANOVA and Tukey or Kruskall-Wallis and Dunn statistical tests were conducted (α=.05). Results PDL promoted a higher apical percentage of volume increase, and lower percentage of debris and untouched root canal surface than PDR 25.06 preparation in entire canal and in all thirds (P<.05). Apical enlargement with PDL 35.05 and PDR 35.05 produced a higher percentage of volume increase in the apical region in relation to the initial preparation (P<.05). PDR 35.05 and PDL 35.05 showed similar results in relation to percentage of debris and untouched root canal surface in entire canal and in all thirds (P>.05). Centralization and transportation showed no difference (P>.05). PDR required less time to perform preparation and apical enlargement (P<.05). Conclusions The apical enlargement 35.05 with CM heat-treatment instruments using reciprocating and rotary motion reduced the percentage of debris and untouched root canal surface, without causing deviations or procedural errors. The protocol of greater apical enlargement favors the cleaning of the root canals in both kinematics. Preparation by the reciprocating system was faster than by the rotary system.


Subject(s)
Humans , Titanium , Root Canal Preparation/instrumentation , Dental Instruments , Dental Pulp Cavity/diagnostic imaging , Nickel , Reference Values , Surface Properties , Materials Testing , Reproducibility of Results , Analysis of Variance , Statistics, Nonparametric , Dental Pulp Cavity/anatomy & histology , Equipment Design , X-Ray Microtomography , Hot Temperature
8.
Braz. dent. j ; 29(5): 452-458, Sept.-Oct. 2018. tab, graf
Article in English | LILACS | ID: biblio-974181

ABSTRACT

Abstract This study evaluated physicochemical properties, cytotoxicity and bioactivity of MTA Angelus (MTA), calcium silicate-based cement (CSC) and CSC with 30% Ytterbium oxide (CSC/Yb2O3). Setting time was evaluated using Gilmore needles. Compressive strength was evaluated in a mechanical machine. Radiopacity was evaluated using radiographs of materials and an aluminum scale. Solubility was evaluated after immersion in water. Cell viability was evaluated by means of MTT assay and neutral red staining, and the mineralization activity by using alkaline phosphatase activity and Alizarin Red staining. The data were submitted to ANOVA, Tukey and Bonferroni tests (5% significance). The bioactive potential was evaluated by scanning electron microscopy. The materials presented similar setting time. MTA showed the lowest compressive strength. MTA and CSC/Yb2O3 presented similar radiopacity. CSC/Yb2O3 showed low solubility. Saos-2 cell viability tests showed no cytotoxic effect, except to 1:1 dilution in NR assay which had lower cell viability when compared to the control. ALP at 1 and 7 days was similar to the control. MTA and CSC had greater ALP activity at 3 days when compared to control. All the materials present higher mineralized nodules when compared with the control. SEM analysis showed structures suggesting the presence of calcium phosphate on the surface of materials demonstrating bioactivity. Ytterbium oxide proved to be a properly radiopacifying agent for calcium silicate-based cement since it did not affected the physicochemical and biological properties besides preserving the bioactive potential of this material.


Resumo Este estudo avaliou as propriedades físico-químicas, citotoxicidade e bioatividade do MTA Angelus (MTA), cimento à base de silicato de cálcio (CSC) e CSC com 30% de óxido de itérbio (CSC/Yb2O3). O tempo de presa foi avaliado usando agulhas Gilmore. A resistência à compressão foi avaliada em uma máquina mecânica. A radiopacidade foi avaliada utilizando radiografias dos materiais e uma escala de alumínio. A solubilidade foi avaliada após imersão em água. A viabilidade celular foi avaliada por meio do ensaio MTT e coloração de vermelho neutro (NR), e a atividade de mineralização por meio da atividade da fosfatase alcalina e a coloração com Vermelho de Alizarina. Os dados foram submetidos aos testes ANOVA, Tukey e Bonferroni (5% de significância). O potencial bioativo foi avaliado por microscopia eletrônica de varredura. Os materiais apresentaram tempo de presa semelhante. O MTA mostrou menor resistência à compressão. MTA e CSC/Yb2O3 apresentaram radiopacidade semelhante. CSC/Yb2O3 apresentou menores valores de solubilidade. A viabilidade celular realizada pelos ensaios de MTT e NR não revelaram efeitos ctotóxicos em todas as diluições, exceto na diluição 1:1 no NR, o qual mostrou baixa viabilidade celular (p<0.05) em todos materiais testados quando comparado ao controle. A atividade de ALP em 1 e 7 dias foi similar ao controle (p>0.05). MTA e CSC tiveram significante aumento na atividade de ALP aos 3 dias quando comarados ao controle (p>0.05). Todos os materiais apresentaram grande produção de nódulos mineralizados quando comparados ao controle (P<0.05). A análise da SEM mostrou estruturas que sugerem a presença de depósitos de fosfato de cálcio na superfície dos materiais demonstrando bioatividade. O Yb2O3 mostrou ser um agente radiopacificador adequado em cimentos à base de silicato de cálcio uma vez que não afetou as propriedades físico-químicas e biológicas e ainda preservou o potencial bioativo desse material.


Subject(s)
Oxides/chemistry , Ytterbium/chemistry , Bismuth/chemistry , Silicates/chemistry , Calcium Compounds/chemistry , Dental Cements/chemistry , Solubility , Staining and Labeling , Materials Testing , Microscopy, Electron, Scanning , Cell Survival , Aluminum Compounds/chemistry , Compressive Strength , Chemical Phenomena
9.
J. appl. oral sci ; 26: e20170465, 2018. tab, graf
Article in English | LILACS, BBO | ID: biblio-893724

ABSTRACT

Abstract Objective: To evaluate the absorption/fluid uptake, solubility and porosity of White mineral trioxide aggregate (MTA) Angelus, Biodentine (BIO), and zinc oxide-eugenol (ZOE). Material and Methods: Solubility was evaluated after immersion in distilled water for 7 and 30 days. Porosity was evaluated using digital inverted microscope, scanning electron microscope (SEM) and micro-computed tomography (micro-CT). For the fluid uptake test, specimens were immersed in Hank's balanced salt solution (HBSS) for 1, 7, 14 and 28 days. Fluid absorption, solubility and porosity of the materials were measured after each period. Statistical evaluation was performed using one-way analysis of variance (ANOVA) and Tukey tests, with a significance level at 5%. Results: After 7 and 30 days, BIO showed the highest solubility (p<0.05). All methods demonstrated that MTA had total porosity higher than BIO and ZOE (p<0.05). Micro-CT analysis showed that MTA had the highest porosity at the initial period, after its setting time (p<0.05). After 7 and 30 days, ZOE had porosity lower than MTA and BIO (p<0.05). Absorption was similar among the materials (p>0.05), and higher fluid uptake and solubility were observed for MTA in the fluid uptake test (p<0.05). Conclusions: BIO had the highest solubility in the conventional test and MTA had higher porosity and fluid uptake. ZOE had lower values of solubility, porosity and fluid uptake. Solubility, porosity and fluid uptake are related, and the tests used provided complementary data.


Subject(s)
Oxides/chemistry , Zinc Oxide-Eugenol Cement/chemistry , Silicates/chemistry , Calcium Compounds/chemistry , Aluminum Compounds/chemistry , Solubility , Surface Properties , Time Factors , Materials Testing , Water/chemistry , Microscopy, Electron, Scanning , Reproducibility of Results , Analysis of Variance , Porosity , Drug Combinations , X-Ray Microtomography , Immersion
10.
J. appl. oral sci ; 25(4): 374-380, July-Aug. 2017. tab, graf
Article in English | LILACS, BBO | ID: biblio-893632

ABSTRACT

Abstract Objective To evaluate solubility, dimensional stability, filling ability and volumetric change of root-end filling materials using conventional tests and new Micro-CT-based methods. Material and Methods Solubility (loss of mass) after 7 and 30 days, and dimensional stability (in mm) were evaluated in accordance with Carvalho-Junior, et al. 7 (2007). The filling ability and volumetric change (in mm3) were evaluated by Micro-CT (Bruker-MicroCT, Kontich, Belgium) using resin models with cavities 3 mm deep and 1 mm in diameter. The cavities were filled with materials to evaluate filling ability, and then scanned by Micro-CT. After 7 and 30 days immersed in distilled water, the filled cavities were scanned again to evaluate the volumetric change. MTA Angelus (MTA), Biodentine (BIO) and zinc oxide-eugenol cement (ZOE) were evaluated. Data were submitted to analysis of variance (ANOVA) and Tukey's test with 5% significance level. Results The results suggested correlated or complementary data between the proposed tests. At 7 days, BIO showed higher solubility and at 30 days, showed higher volumetric change in comparison with MTA (p<0.05). With regard to volumetric change, the tested materials were similar (p>0.05) at 7 days. At 30 days, they presented similar solubility. BIO and MTA showed higher dimensional stability than ZOE (p<0.05). ZOE and BIO showed higher filling ability (p<0.05). Conclusions ZOE presented a higher dimensional change, and BIO had greater solubility after 7 days. BIO presented filling ability and dimensional stability, but greater volumetric change than MTA after 30 days. Micro-CT can provide important data on the physicochemical properties of materials complementing conventional tests.


Subject(s)
Oxides/chemistry , Root Canal Filling Materials/chemistry , Zinc Oxide-Eugenol Cement/chemistry , Bismuth/chemistry , Silicates/chemistry , Calcium Compounds/chemistry , X-Ray Microtomography/methods , Reference Values , Solubility , Time Factors , Materials Testing , Reproducibility of Results , Analysis of Variance , Dental Models
11.
Braz. dent. j ; 28(1): 51-56, Jan.-Feb. 2017. tab, graf
Article in English | LILACS | ID: biblio-839120

ABSTRACT

Abstract The aim of this study was to evaluate the physicochemical properties and the apical dentin bond strength of the tricalcium silicate-based Biodentine in comparison to white MTA and zinc oxide eugenol-based cement (ZOE). Setting time and radiopacity were evaluated according to ISO 6876:2012 specification. Final setting time, compressive strength and pH were also assessed. Material’s bond strength to the apical root canal dentin was measured by the push-out assay. Data were analyzed by ANOVA and Tukey-Krammer post-hoc test. Biodentine presented the shortest initial (16.2±1.48 min) and final setting time (35.4±5.55 min). Radiopacity of Biodentine (2.79±0.27 mmAl) does not agree with ISO 6876:2012 specifications. On the other hand, Biodentine showed higher compressive strength after 21 days (37.22±5.27 MPa) and higher dentin bond strength (11.2±2.16 MPa) in comparison to white MTA (27.68±3.56 MPa for compressive strength and 2.98±0.64 MPa for bond strength) (p<0.05). Both MTA and Biodentine produced an alkaline environment (approximately pH 10) (p>0.05) compared to ZOE (pH 7). It may be concluded that Biodentine exhibited faster setting, higher long-term compressive strength and bond strength to the apical dentin than MTA and ZOE.


Resumo O objetivo deste estudo foi avaliar as propriedades físico-químicas e a resistência de união à dentina apical do cimento Biodentine em comparação ao MTA branco e cimento à base de óxido de zinco e eugenol (OZE). O tempo de presa e a radiopacidade foram avaliados de acordo com as especificações ISO 6876:2012. O tempo de presa final, a resistência à compressão e o pH também foram avaliados. A resistência de união dos materiais à dentina apical do canal radicular foi avaliada por meio do ensaio push-out. Dados foram analisados por ANOVA e teste complementar de Tukey-Krammer. Biodentine apresentou o menor tempo de presa inicial (16,2±1,48 min) e final (35,4±5,55 min). Os valores de radiopacidade do Biodentine (2,79±0,27 mmAl) não estão de acordo com as especificações ISO 6876:2012. Por outro lado, este material apresentou maior resistência à compressão após 21 dias (37,22±5,27 MPa) e maiores valores de adesão à dentina (11,2±2,16 MPa) em comparação ao MTA branco (27,68±3,56 MPa de resistência à compressão e 2,98±0,64 MPa de resistência de união) (p>0.05). Ambos os materiais produziram ambiente alcalino (aproximadamente 10) (p>0.05) em comparação ao OZE (pH 7). Pode-se concluir que o Biodentine demonstrou endurecimento mais rápido e apresentou maior resistência à compressão e resistência de união à dentina apical do que MTA e OZE.


Subject(s)
Humans , Calcium Compounds/chemistry , Dentin/chemistry , Silicates/chemistry , Materials Testing , Root Canal Filling Materials
12.
Braz. dent. j ; 28(1): 65-71, Jan.-Feb. 2017. tab, graf
Article in English | LILACS | ID: biblio-839107

ABSTRACT

Abstract The aim of this study was to evaluate the cytotoxicity and bioactivity of calcium silicate-based cements combined with niobium oxide (Nb2O5) micro and nanoparticles, comparing the response in different cell lines. This evaluation used four cell lines: two primary cultures (human dental pulp cells - hDPCs and human dental follicle cells - hDFCs) and two immortalized cultures (human osteoblast-like cells - Saos-2 and mouse periodontal ligament cells - mPDL). The tested materials were: White Portland Cement (PC), mineral trioxide aggregate (MTA), white Portland cement combined with microparticles (PC/Nb2O5µ) or nanoparticles (PC/Nb2O5n) of niobium oxide (Nb2O5). Cytotoxicity was evaluated by the methylthiazolyldiphenyl-tetrazolium bromide (MTT) and trypan blue exclusion assays and bioactivity by alkaline phosphatase (ALP) enzyme activity. Results were analyzed by ANOVA and Tukey test (a=0.05). PC/Nb2O5n presented similar or higher cell viability than PC/Nb2O5µ in all cell lines. Moreover, the materials presented similar or higher cell viability than MTA. Saos-2 exhibited high ALP activity, highlighting PC/Nb2O5µ material at 7 days of exposure. In conclusion, calcium silicate cements combined with micro and nanoparticles of Nb2O5 presented cytocompatibility and bioactivity, demonstrating the potential of Nb2O5 as an alternative radiopacifier agent for these cements. The different cell lines had similar response to cytotoxicity evaluation of calcium silicate cements. However, bioactivity was more accurately detected in human osteoblast-like cell line, Saos-2.


Resumo O objetivo deste estudo foi avaliar a citotoxicidade e bioatividade de cimentos à base de silicato de cálcio associados com óxido de nióbio (Nb2O5) micro e nanoparticulados, e comparar a resposta em diferentes linhagens celulares. Foram utilizadas quatro linhagens celulares: duas culturas primárias (células da polpa dentária humana - hDPCs e células do folículo dentário humano - hDFCs) e duas culturas imortalizadas (células osteoblásticas humanas - Saos-2 e células do ligamento periodontal de ratos - mPDL). Os materiais analisados foram: Cimento Portland branco (PC); Agregado trióxido mineral (MTA); PC associado com micropartículas (PC/Nb2O5µ) ou nanopartículas (PC/Nb2O5n) de óxido de nióbio (Nb2O5). A citotoxicidade foi avaliada pelos ensaios de brometo de metil-tiazolil-difeniltetrazólio (MTT) e azul de tripan, e a bioatividade pela atividade da enzima fosfatase alcalina (ALP). Os resultados foram analisados por ANOVA e teste de Tukey (a=0,05). O grupo do PC/Nb2O5n apresentou viabilidade celular semelhante ou maior do que o grupo do PC/Nb2O5μ em todas as linhagens celulares. Além disso, ambos os grupos apresentaram viabilidade celular semelhante ou maior do que o MTA. Saos-2 apresentaram maior atividade de ALP, com destaque para o material PC/Nb2O5μ aos 7 dias de exposição. Concluiu-se que cimentos de silicato de cálcio associados com Nb2O5 micro ou nanoparticulado apresentaram citocompatibilidade e bioatividade, demonstrando potencial do Nb2O5 como agente radiopacificador alternativo para estes cimentos. As linhagens celulares estudadas apresentaram resposta semelhante na avaliação da citotoxicidade de cimentos de silicato de cálcio. No entanto, a bioatividade é melhor detectada na linhagem de células osteoblásticas humanas, Saos-2.


Subject(s)
Humans , Animals , Mice , Oxides/pharmacology , Silicates/pharmacology , Calcium Compounds/pharmacology , Dental Cements/pharmacology , Niobium/pharmacology , Cell Line , Alkaline Phosphatase/metabolism
13.
Braz. dent. j ; 27(5): 508-514, Sept.-Oct. 2016. tab
Article in English | LILACS | ID: biblio-828050

ABSTRACT

Abstract Mineral trioxide aggregate (MTA) and Portland cement (PC) are calcium silicate cements. They have similar physicochemical, mechanical and biological properties. The addition of zirconium oxide (ZrO2) to PC provides radiopacity. Silver nanoparticles (AgNPs) may improve some properties of cements. The aim of this study was to evaluate the effect of AgNPs on physicochemical/mechanical properties and antibacterial activity of white MTA (WMTA) and PC associated with ZrO2. The following materials were evaluated: WMTA; PC 70% + ZrO2 30%; WMTA+ AgNPs; and PC 70% + ZrO2 30% + AgNPs. The study evaluated radiopacity, setting time, pH, compressive strength and solubility. For radiopacity analysis, radiographs were made alongside an aluminum (Al) step wedge. To evaluate the antibacterial activity, direct contact test was performed on planktonic cells and Enterococcus faecalis biofilm induced on bovine root dentin for 14 days. The experimental periods were 5 and 15 h. Data were obtained as CFU mL-1. The obtained data were submitted to ANOVA and Tukey tests (p<0.05). The addition of AgNPs to WMTA increased the pH, lowered the solubility and the initial and final setting times. The addition of AgNPs to PC/ZrO2 maintained the pH, lowered the solubility, and increased the setting time and compressive strength. The radiopacity of all materials was higher than 4 mmAl. The addition of AgNPs promoted an increase in antibacterial activity for calcium silicate cements and favored the physicochemical and mechanical properties of the materials.


Resumo Mineral trióxido agregado (MTA) e cimento Portland (CP) são cimentos à base de silicato de cálcio. Eles apresentam propriedades físico-químicas, mecânica e biológicas semelhantes. A adição de óxido de zircônio (ZrO2) ao CP confere radiopacidade. Nanopartículas de prata (NPsAg) podem melhorar propriedades dos cimentos. O objetivo deste estudo foi avaliar o efeito da NPsAg nas propriedades físico-químicas, mecânicas e na atividade antibacteriana do MTA branco (WMTA) e CP associado ao ZrO2. Os seguintes materiais foram avaliados: WMTA; CP 70% + ZrO2 30%; ; WMTA + NPsAg; CP 70% + ZrO2 30% + NPsAg. Foram avaliados a radiopacidade, tempo de presa, pH, resistência à compressão e solubilidade. Para análise da radiopacidade foram tiradas radiografias ao lado de uma escala de alumínio (Al). Para avaliar a atividade antibacteriana, foi realizado o teste de contato direto sobre células planctônicas e biofilme de Enterococcus faecalis formado em dentina radicular bovina durante 14 dias. Os períodos experimentais foram 5 e 15 h. Os dados foram obtidos como UFC mL-1 e submetidos aos testes de ANOVA e Tukey (p<0,05). A adição de NPsAg ao WMTA elevou o pH, diminuiu a solubilidade e os tempos de presa inicial e final. A adição de NPsAg ao CP/ZrO2 manteve o pH, diminuiu a solubilidade, aumentou o tempo de presa e a resistência à compressão. A radiopacidade de todos os materiais foi maior do que 4 mmAl. A adição de NPsAg promoveu um aumento da atividade antibacteriana dos cimentos à base de silicato de cálcio e favoreceu as propriedades físico-químicas e mecânicas dos materiais.


Subject(s)
Anti-Bacterial Agents/pharmacology , Calcium Compounds/chemistry , Dental Cements/chemistry , Metal Nanoparticles , Silicates/chemistry , Silver/chemistry , Anti-Bacterial Agents/chemistry
14.
Braz. dent. j ; 27(3): 332-335, May-June 2016. tab
Article in English | LILACS | ID: lil-782822

ABSTRACT

Abstract The aim of this study was to evaluate the bond strength of root-end filling materials. Forty 2-mm-thick slices were obtained from human single-rooted teeth. After root canal preparation using a 1.5 mm diameter cylindrical drill, the dentinal walls were prepared by diamond ultrasonic tip (CVD T0F-2). The specimens were divided according the material (n=10): MTA Angelus (MTAA), MTA Sealer (MTAS, experimental), Sealer 26 (S26) and zinc oxide and eugenol cement (ZOE). The push-out test was performed in a mechanical test machine (EMIC DL 2000) at 1 mm/min speed. The failure type was evaluated by stereomicroscopy. The results were subjected to ANOVA and Tukey test, at 5% significance level. MTAA (19.18 MPa), MTAS (19.13 MPa) and S26 (15.91 MPa) showed higher bond strength (p<0.05). ZOE (9.50 MPa) showed the least bond strength values (p<0.05). Adhesive failure was prevalent in all groups, except for ZOE, which showed mixed failures. It was concluded that root-end filling materials MTA Angelus, MTA Sealer and Sealer 26 showed higher bond strength to dentinal walls than zinc oxide and eugenol cement after retrograde preparation.


Resumo O objetivo deste estudo foi avaliar a resistência de união de materiais retrobturadores. Quarenta fatias de 2 mm de espessura foram obtidas a partir de dentes unirradiculares humanos. Após o preparo do canal radicular usando uma broca cilíndrica de 1,5 mm de diâmetro, as paredes de dentina foram preparadas usando uma ponta de ultra-som diamantada (CVD T0F-2). As amostras foram divididas de acordo com os materiais (n=10): MTA Angelus (MTAA), MTA Sealer (MTAS, experimental), Sealer 26 (S26) e óxido de zinco e eugenol (ZOE). O teste de push-out foi realizado utilizando uma máquina de ensaios mecânicos (EMIC DL 2000) com velocidade de 1 mm/min. O tipo de falha foi avaliado em estereomicroscópio. Os resultados foram submetidos a ANOVA e teste de Tukey, com níveis de variância de 5%. MTAA (19,18 MPa), MTAS (19,13 MPa) e S26 (15,91 MPa) apresentaram os maiores valores de resistência de união (p<0,05). ZOE (9,50 MPa) apresentou os menores valores de resistência de união (p<0,05). A falha adesiva foi prevalente em todos os grupos, com exceção do ZOE, que apresentou falhas mistas. Concluiu-se que os materiais retrobturadores MTA Angelus, MTA Sealer e Sealer 26 apresentam maior resistência de união às paredes dentinárias que o óxido de zinco e eugenol após o preparo retrógrado.


Subject(s)
Humans , Materials Testing , Root Canal Filling Materials/chemistry , Root Canal Preparation
15.
J. appl. oral sci ; 24(3): 204-210, tab, graf
Article in English | LILACS, BBO | ID: lil-787546

ABSTRACT

ABSTRACT Objective Mineral Trioxide Aggregate (MTA) is a calcium silicate cement composed of Portland cement (PC) and bismuth oxide. Hydroxyapatite has been incorporated to enhance mechanical and biological properties of dental materials. This study evaluated physicochemical and mechanical properties and antibiofilm activity of MTA and PC associated with zirconium oxide (ZrO2) and hydroxyapatite nanoparticles (HAn). Material and Methods White MTA (Angelus, Brazil); PC (70%)+ZrO2 (30%); PC (60%)+ZrO2 (30%)+HAn (10%); PC (50%)+ZrO2 (30%)+HAn (20%) were evaluated. The pH was assessed by a digital pH-meter and solubility by mass loss. Setting time was evaluated by using Gilmore needles. Compressive strength was analyzed by mechanical test. Samples were radiographed alongside an aluminum step wedge to evaluate radiopacity. For the antibiofilm evaluation, materials were placed in direct contact with E. faecalis biofilm induced on dentine blocks. The number of colony-forming units (CFU mL-1) in the remaining biolfilm was evaluated. The results were submitted to ANOVA and the Tukey test, with 5% significance. Results There was no difference in pH levels of PC+ZrO2, PC+ZrO2+HAn (10%) and PC+ZrO2+HAn (20%) (p>0.05) and these cements presented higher pH levels than MTA (p<0.05). The highest solubility was observed in PC+ZrO2+HAn (10%) and PC+ZrO2+HAn (20%) (p<0.05). MTA had the shortest initial setting time (p<0.05). All the materials showed radiopacity higher than 3 mmAl. PC+ZrO2 and MTA had the highest compressive strength (p<0.05). Materials did not completely neutralize the bacterial biofilm, but the association with HAn provided greater bacterial reduction than MTA and PC+ZrO2 (p<0.05) after the post-manipulation period of 2 days. Conclusions The addition of HAn to PC associated with ZrO2 harmed the compressive strength and solubility. On the other hand, HAn did not change the pH and the initial setting time, but improved the radiopacity (HAn 10%), the final setting time and the E. faecalis antibiofilm activity of the cement.


Subject(s)
Oxides/chemistry , Zirconium/chemistry , Enterococcus faecalis/drug effects , Silicates/chemistry , Durapatite/chemistry , Calcium Compounds/chemistry , Aluminum Compounds/chemistry , Biofilms/drug effects , Nanoparticles/chemistry , Oxides/pharmacology , Solubility , Time Factors , Zirconium/pharmacology , Bismuth/pharmacology , Bismuth/chemistry , Materials Testing , Colony Count, Microbial , Analysis of Variance , Enterococcus faecalis/growth & development , Silicates/pharmacology , Durapatite/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Compressive Strength , Dental Cements/pharmacology , Dental Cements/chemistry , Drug Combinations , Hydrogen-Ion Concentration
16.
J. appl. oral sci ; 24(2): 121-125, Mar.-Apr. 2016. tab
Article in English | LILACS | ID: lil-779910

ABSTRACT

ABSTRACT Objective To evaluate solubility and sealing ability of Mineral Trioxide Aggregate (MTA) and root-end filling materials. Material and Methods The materials evaluated were: MTA, Calcium Silicate Cement with zirconium oxide (CSC/ZrO2), and zinc oxide/eugenol (ZOE). Solubility test was performed according to ANSI/ADA. The difference between initial and final mass of the materials was analyzed after immersion in distilled water for 7 and 30 days. Retrograde cavities in human teeth with single straight root canal were performed by using ultrasonic tip CVD 9.5107-8. The cavities were filled with the evaluated materials to evaluate sealing ability using the bacterial leakage test with Enterococcus faecalis. Bacterial leakage was evaluated every 24 hours for six weeks observing the turbidity of Brain Heart infusion (BHI) medium in contact with root apex. Data were submitted to ANOVA followed by Tukey tests (solubility), and Kruskal-Wallis and Dunn tests (sealing ability) at a 5% significance level. Results For the 7-day period, ZOE presented highest solubility when compared with the other groups (p<0.05). For the 30-day period, no difference was observed among the materials. Lower bacterial leakage was observed for MTA and CSC/ZrO2, and both presented better results than ZOE (p<0.05). Conclusion MTA and CSC/ZrO2 presented better bacterial sealing capacity, which may be related to lower initial solubility observed for these materials in relation to ZOE.


Subject(s)
Humans , Aluminum Compounds/chemistry , Calcium Compounds/chemistry , Dental Leakage/microbiology , Eugenol/chemistry , Oxides/chemistry , Root Canal Filling Materials/chemistry , Silicates/chemistry , Zinc Oxide/chemistry , Zirconium/chemistry , Analysis of Variance , Dental Cavity Lining , Dental Pulp Cavity/drug effects , Dental Pulp Cavity/microbiology , Drug Combinations , Enterococcus faecalis , Materials Testing , Reproducibility of Results , Retrograde Obturation/methods , Solubility , Statistics, Nonparametric , Time Factors
17.
Braz. oral res. (Online) ; 30(1): e48, 2016. tab, graf
Article in English | LILACS | ID: biblio-952020

ABSTRACT

Abstract Several calcium silicate-based biomaterials have been developed in recent years, in addition to Mineral Trioxide Aggregate (MTA). The aim of this study was to evaluate the cytotoxicity, genotoxicity and apoptosis/necrosis in human osteoblast cells (SAOS-2) of pure calcium silicate-based cements (CSC) and modified formulations: modified calcium silicate-based cements (CSCM) and three resin-based calcium silicate cements (CSCR1) (CSCR 2) (CSCR3). The following tests were performed after 24 hours of cement extract exposure: methyl-thiazolyl tetrazolium (MTT), apoptosis/necrosis assay and comet assay. The negative control (CT-) was performed with untreated cells, and the positive control (CT+) used hydrogen peroxide. The data for MTT and apoptosis were submitted to analysis of variance and Bonferroni's posttest (p < 0.05), and the data for the comet assay analysis, to the Kruskal-Wallis and Dunn tests (p < 0.05). The MTT test showed no significant difference among the materials in 2 mg/mL and 10 mg/mL concentrations. CSCR3 showed lower cell viability at 10 mg/mL. Only CSC showed lower cell viability at 50 mg/mL. CSCR1, CSCR2 and CSCR3 showed a higher percentage of initial apoptosis than the control in the apoptosis test, after 24 hours exposure. The same cements showed no genotoxicity in the concentration of 2 mg/mL, with the comet assay. CSC and CSCR2 were also not genotoxic at 10 mg/mL. All experimental materials showed viability with MTT. CSC and CSCR2 presented a better response to apoptosis and genotoxicity evaluation in the 10 mg/mL concentration, and demonstrated a considerable potential for use as reparative materials.


Subject(s)
Humans , Osteoblasts/drug effects , Silicates/toxicity , Calcium Compounds/toxicity , Dental Cements/toxicity , Oxides/toxicity , Tetrazolium Salts , Biocompatible Materials/toxicity , Materials Testing , Cell Survival/drug effects , Cells, Cultured , Reproducibility of Results , Analysis of Variance , Apoptosis/drug effects , Aluminum Compounds/toxicity , Comet Assay , Cell Proliferation/drug effects , Drug Combinations , Formazans , Necrosis/chemically induced
18.
J. appl. oral sci ; 23(5): 467-471, Sept.-Oct. 2015. graf
Article in English | LILACS, BBO | ID: lil-764155

ABSTRACT

Mineral Trioxide Aggregate (MTA) is a calcium silicate-based material. New sealers have been developed based on calcium silicate as MTA Fillapex and MTA Plus.Objective The aim of this study was to evaluate biocompatibility and bioactivity of these two calcium silicate-based sealers in culture of human dental pulp cells (hDPCs).Material and Methods The cells were isolated from third molars extracted from a 16-year-old patient. Pulp tissue was sectioned into fragments with approximately 1 mm3 and kept in supplemented medium to obtain hDPCs adherent cultures. Cell characterization assays were performed to prove the osteogenic potential. The evaluated materials were: MTA Plus (MTAP); MTA Fillapex (MTAF) and FillCanal (FC). Biocompatibility was evaluated with MTT and Neutral Red (NR) assays, after hDPCs exposure for 24 h to different dilutions of each sealer extract (1:2, 1:3 and 1:4). Unexposed cells were the positive control (CT). Bioactivity was assessed by alkaline phosphatase (ALP) enzymatic assay in cells exposed for one and three days to sealer extracts (1:4 dilution). All data were analyzed by ANOVA and Tukey post-test (p≤0.05%).Results MTT and NR results showed suitable cell viability rates for MTAP at all dilutions (90-135%). Cells exposed to MTAF and FC (1:2 and 1:4 dilutions) showed significant low viability rate when compared to CT in MTT. The NR results demonstrated cell viability for all materials tested. In MTAP group, the cells ALP activity was similar to CT in one and three days of exposure to the material. MTAF and FC groups demonstrated a decrease in ALP activity when compared to CT at both periods of cell exposure.Conclusions The hDPCs were suitable for the evaluation of new endodontic materialsin vitro. MTAP may be considered a promising material for endodontic treatments.


Subject(s)
Humans , Adolescent , Aluminum Compounds , Biocompatible Materials , Calcium Compounds , Cell Survival/drug effects , Dental Pulp/drug effects , Oxides , Root Canal Filling Materials , Silicates , Alkaline Phosphatase/analysis , Alkaline Phosphatase/drug effects , Analysis of Variance , Barium Sulfate , Bismuth , Borates , Cells, Cultured , Drug Combinations , Eugenol , Formazans , Materials Testing , Reproducibility of Results , Resins, Synthetic , Statistics, Nonparametric , Tetrazolium Salts , Time Factors , Zinc Oxide
19.
Braz. dent. j ; 26(4): 342-346, July-Aug. 2015. tab
Article in English | LILACS | ID: lil-756381

ABSTRACT

Endodontic irrigation aims to clean and disinfect the root canal system. Passive ultrasonic irrigation (PUI) is based on the use of an ultrasound-activated instrument into the root canal filled with irrigant. The aim of this study was to evaluate, ex vivo, the effectiveness of PUI in eliminating Enterococcus faecalis from root canals. Seventy-five extracted human single-root teeth were used. After root canal preparation, specimens were inoculated with E. faecalis and incubated at 37 °C for 21 days. Specimens were distributed into five groups (n=15), according to the irrigation method: PUI + saline solution (PUI/SS); PUI + 1% NaOCl (PUI/NaOCl); conventional needle irrigation (CNI) + saline solution (CNI/SS); CNI + 1% NaOCl (CNI/NaOCl); No irrigation (control). Microbiological samples were collected at three time points: initial (21 days after inoculation), post-irrigation (immediately after irrigation), and final (7 days after irrigation). Data were obtained in CFU mL-1 and subjected to analysis by ANOVA and Tukey's tests at 5% significance level. The post-irrigation samples did not demonstrate statistical difference between PUI/SS and CNI/SS nor between PUI/NaOCl and CNI/NaOCl (p>0.05), but PUI/NaOCl and CNI/NaOCl had lower CFU mL-1 number than the other groups (p>0.05). Statistically significant difference was observed between the initial and post-irrigation samples and between the post-irrigation and final samples (p<0.05) in all groups, except in the control. The final samples of all groups presented bacterial counts similar to the initial samples. PUI or CNI with 1% NaOCl contribute to disinfection, but are unable to eradicate E. faecalis from the root canal system.

.

A irrigação endodôntica visa à limpeza e desinfecção do sistema de canais radiculares. A irrigação ultrassônica passiva (IUP) baseia-se na utilização de um instrumento ultrassônico ativado no interior do canal radicular preenchido com a solução irrigadora. O objetivo deste estudo foi avaliar, ex vivo, a eficácia da IUP na eliminação de Enterococcus faecalis dos canais radiculares. Foram usados 75 dentes humanos unirradiculares extraídos. Após o preparo biomecânico dos canais radiculares, foram inoculados com E. faecalis e incubados a 37 °C por 21 dias. Os espécimes foram distribuídos em cinco grupos (n=15), de acordo com o método de irrigação: IUP + solução salina (IUP/SS); IUP + NaOCl a 1% (IUP/NaOCl); irrigação manual convencional (IMC) + solução salina (IMC/sol); IMC + NaOCl a 1% (IMC/NaOCl); Sem irrigação (controle). Foram realizadas três coletas microbiológicas: inicial (21 dias após a inoculação), pós-irrigação (imediatamente após a irrigação), e final (7 dias após a irrigação). Os dados foram obtidos em UFC mL-1 e submetidos aos testes de ANOVA e Tukey. A coleta pós-irrigação não demonstrou diferença estatisticamente significante entre IUP/SS e IMC/SS nem entre IUP/NaOCl e IMC/NaOCl (p>0,05), mas IUP/NaOCl e IMC/NaOCl apresentaram menor número de UFC mL-1 comparado aos outros grupos (p>0,05). Diferença estatisticamente significante foi observada entre as coletas iniciais e pós-irrigação e entre a pós-irrigação e a coleta final (p<0,05) em todos os grupos, exceto no controle. Na coleta final, todos os grupos apresentaram contagem bacteriana similar à coleta inicial. IUP ou IMC com NaOCl a 1% contribuem para a desinfecção, mas não eliminam E. faecalis do sistema de canais radiculares.

.


Subject(s)
Humans , Dental Pulp Cavity/microbiology , Enterococcus faecalis/isolation & purification , Therapeutic Irrigation , Ultrasonic Therapy , Ultrasonics , In Vitro Techniques
20.
Braz. dent. j ; 26(3): 211-215, May-Jun/2015. tab
Article in English | LILACS | ID: lil-751858

ABSTRACT

The aim of this study was to evaluate the fracture resistance of simulated immature teeth after different intra-radicular treatments. Crowns and roots of bovine incisors were cut transversally and removed to simulate immature teeth. Root canal preparation and flaring were performed using a bur in crown-apex and apex-crown direction. The samples were distributed into 5 groups (n=10): Positive control (PoC) - no root canal flaring or filling; Negative control (NeC) - teeth were sectioned and their root canals were flared; Direct anatomical glass fiber post (RaP) - #2 Reforpost main glass fiber post relined with composite resin; Double tapered conical glass fiber posts (ExP) - #3 Exacto glass fiber post; and #2 Reforpost main glass fiber + Reforpin accessory glass fiber posts (RrP). In RaP, ExP and RrP, 4.0-mm apical plugs were done with MTA Angelus. The specimens were embedded in polystyrene resin inside cylinders and the periodontal ligament was simulated with a polyether-based impression material. The specimens were submitted to compressive fracture strength test (0.5 mm/min at 135° relative to the long axis of the tooth) in a servo-hydraulic mechanical testing machine MTS 810. Data were subjected to one-way ANOVA and Dunnett's C or Tukey's tests (α=0.05). The control groups (PoC and NeC) showed lower fracture strength than the experimental groups. NeC presented the lowest resistance and ExP presented the highest resistance among the experimental groups. The flaring procedures produced a detrimental effect on the fracture resistance of the bovine teeth. Glass fiber intra-radicular posts increased significantly the fracture resistance of simulated immature teeth.


O objetivo deste estudo foi avaliar a resistência à fratura de dentes com rizogênese incompleta simulada após diferentes tratamentos intra-radiculares. A rizogênese incompleta foi simulada pelo seccionamento das coroas e raízes de incisivos bovinos. O preparo e alargamento do canal radicular foram realizados utilizando uma broca no sentido coroa-ápice e ápice-coroa. As amostras foram distribuídas (n=10) em cinco grupos: controle positivo (PoC) - sem preparo do canal radicular e sem obturação; controle negativo (NeC) - os dentes foram seccionados e os canais radiculares foram preparados; pino anatômico direto Reforpost #2 (RaP) - pino de fibra de vidro principal + resina composta; pino de fibra de vidro com dupla conicidade (ExP) - pino de fibra de vidro Exacto #3; e pino de fibra de vidro Reforpost #2 + pinos de fibra de vidro acessórios Reforpin (RrP). Nos grupos RaP, ExP e RrP foram realizados plugs apicais de MTA Angelus com 4,0 mm. As amostras foram incluídas em cilindros com resina de poliestireno e o ligamento periodontal foi simulado com material de moldagem à base de poliéter. As amostras foram submetidas ao teste de resistência à fratura, por meio de força compressiva (0,5 mm/min a 135º em relação ao longo eixo do dente) em uma máquina de ensaios mecânicos MTS 810. Os dados foram submetidos à ANOVA a um critério e aos testes para comparações múltiplas Dunnett C ou Tukey HSD (α=0,05). Os grupos controle (PoC e NeC) apresentaram resistência à fratura inferior aos grupos experimentais, sendo o menor valor médio produzido por NeC. ExP produziu os maiores valores de resistência à fratura entre os grupos experimentais. Os procedimentos de preparo e alargamento produziram efeitos prejudiciais na resistência à fratura de dentes bovinos. A utilização de pinos de fibra de vidro intra-radiculares aumentou significativamente a resistência à fratura de dentes com rizogênese incompleta simulada.


Subject(s)
Animals , Cattle , Tooth Fractures , Tooth Root/surgery , Post and Core Technique
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