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Article in Chinese | WPRIM | ID: wpr-279864


<p><b>OBJECTIVE</b>To investigate the significance of Th17/Treg imbalance in the development and treatment of primary immune thrombocytopenia (ITP) in children.</p><p><b>METHODS</b>Thirty-two children diagnosed with ITP between May and August, 2015 and 22 healthy children were enrolled. Flow cytometry was used to determine the Th17/Treg ratio in peripheral blood of healthy children and children with ITP before and after treatment with immunoglobulin.</p><p><b>RESULTS</b>Compared with the patients with ITP before treatment, the healthy children and the patients treated with immunoglobulin had a significantly lower percentage of Th17 cells in CD4+ T cells, a significantly lower Th17/Treg ratio, and a significantly higher percentage of Treg cells in CD4+ T cells in peripheral blood (P<0.05). In the 32 ITP children treated with immunoglobulin, 20 had complete response, 4 had response, and 8 had no response. The patients with complete response had a significantly lower percentage of Th17 cells in CD4+ T cells and a significantly lower Th17/Treg ratio in peripheral blood than the patients without response (P<0.05).</p><p><b>CONCLUSIONS</b>The Th17/Treg imbalance can be found in children with ITP. Immunoglobulin can improve the cellular immune function by regulation of the Th17/Treg ratio. The Th17/Treg ratio may serve as an indicator for assessing the therapeutic effects of ITP.</p>

Child , Child, Preschool , Female , Humans , Infant , Male , Purpura, Thrombocytopenic, Idiopathic , Allergy and Immunology , T-Lymphocytes, Regulatory , Allergy and Immunology , Th17 Cells , Allergy and Immunology
Article in Chinese | WPRIM | ID: wpr-325870


<p><b>OBJECTIVE</b>To observe the anatomy structure of rabbit ear and the effect of different operation methods and post-operative treatments on the formation of hypertrophic scar.</p><p><b>METHODS</b>The experimental animals were 25 New Zealand white rabbits. 6 pieces of full skin specimens were obtained from each of the ears in 5 rabbits for histological examination. 6 full-thickness skin wounds (d = 8 mm) were made on different sites of ventral side of each ear in the other 20 rabbits. The total number of the wounds was 240. 120 wounds in 10 rabbits were divided into 4 groups randomly to receive different treatments on day 7 postoperatively. No treatment was performed in the other 120 wounds. The wounds healing and the scar formation were observed for six months. The scars were harvested 4 weeks and 8 weeks after operation for pathologic examination and measurement of scar elevation index (SEI).</p><p><b>RESULTS</b>Histological analysis showed that the anatomy structure was different in different sites of the rabbit ear. The best sites for creating hypertrophic scar model were on the medial margin of the middle- and inferior part of ear. The depth of the wound should reach the cartilage membrane of the ear to facilitate the formation of hypertrophic scar. The second strip crust on day 7 postoperatively enhanced the wounds healing and minimized the scar proliferation and hypertrophy.</p><p><b>CONCLUSIONS</b>There is a close correlation between the anatomy structure of the ear and the creation of hypertrophic scar animal model. The wound site, the depth of wound and the post-operative treatment will affect the formation of hypertrophic scar. The study can help to improve the successful rate of creating hypertrophic scar animal model.</p>

Animals , Cicatrix, Hypertrophic , Disease Models, Animal , Ear, External , Female , Male , Rabbits
Article in Chinese | WPRIM | ID: wpr-240389


<p><b>OBJECTIVE</b>To investigate the pathogenesy deference between multiple and single site keloid by detecting gene mutation of Poly A site of transforming growth factor-beta1 receptor type II (TbetaR II).</p><p><b>METHODS</b>Collecting 20 keloid samples (6 multiple sites keloid samples and 14 single site keloid samples) and extracting DNA from them; designing and synthesizing the primers of Poly A site, then amplifying T1beta II DNA by PCR, analyzing the single strand conformation polymorphism about the products of PCR. After purifying the product of PCR, the site and type of the mutation rate of Poly A site was sequenced directly on the automatic sequencing equipment.</p><p><b>RESULTS</b>It had been found that the Poly A site of TbetaR II in keloid has deletion mutation, its mutation rate in multiple sites keloid was 50% (3/6), in single site keloid 7.1% (1/14). The mutation rate of Poly A site in multiple sites keloid was significant higher than that in single site keloid (P < 0.05)</p><p><b>CONCLUSION</b>It has been supposed that there are some deference in pathogenesy between the multiple and the single site keloid.</p>

Adolescent , Adult , Base Sequence , Child , Child, Preschool , Female , Genetic Vectors , Humans , Keloid , Genetics , Male , Middle Aged , Mutation , Poly A , Genetics , Protein Serine-Threonine Kinases , Genetics , Receptors, Transforming Growth Factor beta , Genetics