Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 31
Filter
Add filters








Year range
1.
Chinese Journal of School Health ; (12): 1876-1881, 2020.
Article in Chinese | WPRIM | ID: wpr-862235

ABSTRACT

Objective@#To systematically review the prevalence of iron-deficiency anemia among children aged 0-14 years in China from 2000 to 2020, and to provide a reference for prevention and controlling of IDA among Chinese children.@*Methods@#CNKI, CBM, WanFang Data, VIP databases, PubMed, Embase and Web of Science were electronically searched to collect crosssectional studies on the prevalence of iron deficiency anemia in children aged 0-14 years of China from 2000 to April 2020. Two reviewers independently conducted literature screening, methodological evaluation and data extraction, and used Stata 13.0 software to combine the data to estimate the prevalence. The Q test and I 2 statistics were used to evaluate the heterogeneity of studies. Begg and Egger test were used to evaluater.@*Results@#A total of 60 articles were included, including 122 771 children, among whom 28 693 were sick. Meta-analysis results showed that the total prevalence rate of children aged 0-14 years in China from 2000 to 2020 was 19.9%. The prevalence rate of girls (18.7%) was higher than that of boys (16.9%), and the difference was statistically significant(P<0.05). The prevalence rate was highest in infancy(30.3%), followed by in early childhood(16.7%). From 2006 to 2010, the prevalence rate of children was the highest(22.6%). In recent years, the prevalence rate of children with iron deficiency anemia was lower than before. Mild anemia was found in 88.7% of the children, and moderate or severe anemia was found in 11.3% of the children. The prevalence rate of children in western China was the highest, 31.9% and 28.3% respectively, and the incidence rate in east China(13.1%), south China (14.0%) and northeast China (16.6%) was relatively low. The prevalence rate of rural children (25.6%) was much higher than that of urban children(9.1%), especially in western rural areas.@*Conclusion@#The prevalence of iron-deficiency anemia among children aged 0-14 years in China from 2000 to 2020 is still high and the differences between different regions are significant, so more attention should be paid to the prevention and treatment of iron-deficiency anemia among infants and children in poor areas.

2.
Frontiers of Medicine ; (4): 438-450, 2019.
Article in English | WPRIM | ID: wpr-771254

ABSTRACT

Cervical cancer (CC) is the fourth most commonly diagnosed female malignancy and a leading cause of cancer-related mortality worldwide, especially in developing countries. Despite the use of advanced screening and preventive vaccines, more than half of all CC cases are diagnosed at advanced stages, when therapeutic options are extremely limited and side effects are severe. Given these circumstances, new and effective treatments are needed. In recent years, exciting progress has been made in immunotherapies, including the rapid development of immune checkpoint inhibitors. Checkpoint blockades targeting the PD-1/PD-L1 axis have achieved effective clinical responses with acceptable toxicity by suppressing tumor progression and improving survival in several tumor types. In this review, we summarize recent advances in our understanding of the PD-1/PD-L1 signaling pathway, including the expression patterns of PD-1/PD-L1 and potential PD-1/PD-L1-related therapeutic strategies for CC.

3.
Chinese Journal of Oncology ; (12): 675-680, 2019.
Article in Chinese | WPRIM | ID: wpr-797946

ABSTRACT

Objective@#To investigate the inhibitory effect of programmed cell death factor 4 (PDCD4) on arsenic trioxide (As2O3)-induced cell growth and nuclear factor kappa B (NF-κB) signaling pathway in neuroblastoma.@*Methods@#The PDCD4 overexpression vector was transfected into neuroblastoma cells and detected by fluorescence quantitative PCR and Western blot. As2O3 was used to treat PDCD4 overexpressing neuroblastoma cells. MTT assay was used to measure the proliferation. Colony formation assay was used to determine the cell clone forming ability. Apoptosis was measured by flow cytometry. Western blot was used to detect the expression of NF-κB p65 and cleaved caspase-3 protein in cells.@*Results@#The transfection of PDCD4 overexpression vector significantly increased the expression level of PDCD4 in neuroblastoma cells. The cell survival rates of the control group, PDCD4 group, As2O3 group and As2O3+ PDCD4 group were 100%, (72.14±5.20)%, (62.58±3.14)% and (40.87±2.47)%, respectively. The colony formation rates in these four groups were (91.25±8.36)%, (65.32±7.14)%, (57.23±5.28)% and (37.14±3.64)%, respectively. In addition, the cell apoptotic rates of these four groups were (3.57±0.24)%, (28.64±3.20)%, (36.41±4.58)% and (49.65±5.27)%, respectively. Therefore, overexpression of PDCD4 in the absence or presence of As2O3 inhibited cell proliferation and clone formation ability, while promoted apoptosis. Furthermore, the expression levels of cleaved caspase-3 in the control group, PDCD4 group, As2O3 group and As2O3+ PDCD4 group were 0.21±0.03, 0.30±0.02, 0.43±0.05 and 0.57±0.06, respectively. And the expression levels of NF-κB p65 protein were 0.68±0.04, 0.52±0.03, 0.43±0.04, and 0.32±0.02, respectively. Compared with the control group, the expression levels of NF-κB p65 protein in PDCD4 group, As2O3 group and As2O3+ PDCD4 group were significantly decreased (P<0.05), whereas the expression level of cleaved Caspase-3 protein was significantly increased (P<0.05). The changes in As2O3+ PDCD4 group were more significant than those in PDCD4 group and As2O3 groups (both P<0.05).@*Conclusion@#PDCD4 enhances the inhibitory effect of As2O3 on the growth and NF-κB signaling pathway in neuroblastoma cells.

4.
Chinese Journal of Oncology ; (12): 516-521, 2019.
Article in Chinese | WPRIM | ID: wpr-810772

ABSTRACT

Objective@#To investigate the effects and mechanisms of miR-144 on proliferation, apoptosis and cisplatin (DDP) resistance of neuroblastoma cells.@*Methods@#Real-time fluorescence quantitative PCR (RT-qPCR) was used to detect the mRNA expressions of miR-144 and MYCN in neuroblastoma cell lines, including SH-SY5Y and SK-N-SH, and human umbilical vein endothelial cells HUVEC. The miR-negative control, miR-144 mimics, si-negative control, si-MYCN, miR-144 mimics and pcDNA, miR-144 mimics and pcDNA-MYCN co-transfected SH-SY5Y cells were described as miR-NC, miR-144, si-NC, si-MYCN, miR-144+ pcDNA and miR-144+ pcDNA-MYCN group, respectively. The half maximal inhibitory concentration (IC50) and cell proliferation were detected by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide (MTT) assay. The protein expressions of MYCN, p21, cyclin D1, Bax, Bcl-2 were analyzed by western blot. Cell apoptosis was detected by flow cytometry. The cell fluorescence activity was detected by double luciferase reporter gene assay.@*Results@#Compared with HUVEC cells, the expressions of miR-144 in neuroblastoma cells SH-SY5Y and SK-N-SH significantly decreased, while the mRNA and protein expression of MYCN significantly increased. The IC50 of DDP was 9.16 μg/ml in SH-SY5Y cells. The absorbance value in 490nm (A490 value) of miR-144 group was 0.30±0.03, significantly lower than 0.46±0.03 of miR-NC group. The cell apoptotic rate of miR-144 group was 26.94%±2.01%, significantly higher than 9.68%±0.52% of miR-NC group. The IC50 value of DDP in miR-144 group was 2.95±0.26, significantly lower than 9.23±0.61 of miR-NC group. The expressions of p21, cyclin D1, Bax, Bcl-2 in miR-NC and miR-144 group were 2.67±0.19, 0.41±0.04, 2.12±0.21, 0.18±0.01 and 1.01±0.07, 1.00±0.06, 1.00±0.05, 1.00±0.06, respectively, with statistical significance (all P<0.05). Knockdown of MYCN showed the similar effects with those of miR-144 overexpression in SH-SYSY cells. MiR-144 significantly inhibited the fluorescence activity of ectopic MYCN expressing cells and negatively regulated the expression of MYCN. Overexpression of MYCN can reverse the effects of miR-144 on proliferation inhibition, apoptosis promotion and sensitization of SH-SY5Y cells to DDP.@*Conclusion@#MiR-144 inhibits proliferation, promotes apoptosis and enhances the sensitivity of neuroblastoma cells to DDP through targeting MYCN, which provides a potential treatment for neuroblastoma.

5.
Herald of Medicine ; (12): 727-730, 2017.
Article in Chinese | WPRIM | ID: wpr-620263

ABSTRACT

Objective To explore the expression of anaplastic lymphoma kinase(ALK) in human ovarian serous adenocarcinoma cell line SKOV3 and to further investigate the effect of crizotinib on SKOV3 cells.Methods The expression of ALK in SKOV3 cells were examined by Western blotting and immunocytochemical methods,and the effect of ALK inhibitor crizotinib on proliferation of SKOV3 cells were evaluated by cell proliferation assay.Results Both Western blotting and immunocytochemical methods showed the expression of ALK in SKOV3 cells.The results of cell proliferation assay suggested that the cell viability of SKOV3 cells was close to 1 when the drug concentration was less than 106 nmol·L-1,and close to 64% when the drug concentration was up to 107nmol·L-1.Conclusion ALK is overexpressed in human ovarian serous adenocarcinoma cell line SKOV3,but SKOV3 cells were insensitive to the therapy of ALK inhibitor crizotinib.

6.
Chinese Mental Health Journal ; (12): 590-594, 2017.
Article in Chinese | WPRIM | ID: wpr-615139

ABSTRACT

Objective:To explore the mediating effect of self-nursing ability between living condition and anxiety in the patients with peptic ulcer caused by helicobacter pylori.Methods:By using questionnaire survey method,300 patients with a peptic ulcer caused by helicobacter pylori were assessed with the exercise of self-care agency (ESCA),Self-rated Health Measurement Scale (SRHMS) and Self-Rating Anxiety Scale (SAS).The result was compared with that of 300 healthy crowd whose general condition was well-matched.The data were processed and analyzed by using SPSS13.0 and AMOS7.0.Results:The total scores of the ESCA,SRHMS and SAS were (101 ± 13),(299 ±35),(56 ±8) respectively.The result of the correlation analysis showed there were varying degree correlation among the disease self-nursing ability,self-rated Health,and anxiety (Ps < 0.01).Bootstrap test showed the size of the mediating effect of self-test health to anxiety was 0.17,the 95% confidence intervals was 0.11-0.26,and the ratio of the mediating effect to the total effect was 0.44.Contusion:It suggests that self-nursing ability may have much strong mediating role between health condition and anxiety.

7.
Article in Chinese | WPRIM | ID: wpr-513415

ABSTRACT

Objective To investigate the efficacy of filiform needle fire acupuncture plus pricking-cupping bloodletting in treating acute ankle sprain in adolescent students.Method One hundred adolescent student outpatients with acute ankle sprainwere randomly allocated to treatment and control groups, 50 cases each.The treatment group received filiform needle fire acupuncture plus pricking-cupping bloodletting at the most obviouspart of ankle tenderness once every other day. The control group received routine protection, a rest, ice compress,compressionand affected limb elevation.Swelling severitywas examined and the AOFAS Ankle Hindfoot Scalescore and the VAS score were recorded at study entry, and one, three and five days and two, four and eight weeks after treatment.Result Swelling severityand the ankle pain score improved in both groups after treatment (P<0.05) and the post-treatment score was lower in the treatment group than in the controlgroup(P<0.05). The total efficacy rate was 100% (50/50) in the treatment group, which was higher than 80% (40/50) in the control group (P<0.05).Conclusion Filiform needle fire acupuncture plus pricking-cupping bloodletting is a better way to treat acute ankle sprain.It can relieve the pain and swelling in short time, shorten treatment time and restore ankle activity in short time to reduce theoccurrence of sequelae.

8.
Journal of Chinese Physician ; (12): 367-370,375, 2017.
Article in Chinese | WPRIM | ID: wpr-606666

ABSTRACT

Objective To explore the roles of miR-10a in the cisplatin resistance in cervical cancer cell lines,and further study the mechanism.Methods The cells were transfected with the mimics of miR10a and its negative control RNA (NC).Methyl thiazolyl tetrazolium (MTF) assay and fluorescence activated cell sorter were used to analyze drug sensitivity and apoptosis after treating with cisplatin,luciferase reporter assay to identify that miR-10a directly targets phosphatase and tensin homologue deleted on chromosome ten (PTEN).The expression of PTEN gene and its protein levels after tansfection was measured,respectively.Results after transfected with miR-10a mimics:(1) The 50% inhibition concentration of Hela cells (7.2 μg/ml) was more than in the NC group (5.6 μg/ml).The 50% inhibition concentration of Siha cells (6.4 μg/ml) was significantly more than in the NC group (3.8 μg/ml) (P<0.05).(2) The apoptosis rates of Hela cells and Siha cells were significantly lower compared to the NC group (P < 0.05).(3)miR-10a might directly target the 3'-untranslated region (3'-UTR) of PTEN,which significantly changed the PTEN protein level (P < 0.05).Conclusions miR-10a may modulate cisplatin-induced apoptosis in cervical cancer by inhibiting PTEN protein level.

9.
Article in Chinese | WPRIM | ID: wpr-506667

ABSTRACT

Objective To monitor and analyze the distribution of pathogenic bacteria from CSF and its drug resistance change in Yangzhou area during 2011-2015 ,so as to provide the latest evidence for clinical rational use of antibacterial drugs .Methods The VITEK 2 automatic microbiological instrument was applied to identify bacteria and conduct the drug susceptibility test .The distri‐bution and drug susceptibility situation of isolated pathogenic bacteria were analyzed by using the WHONET 5 .6 software . Results In 2074 CSF bacterial culture from 2011 to 2015 ,74 strains(3 .57% ) of pathogenic bacteria were isolated ,in which the top three were Acinetobacter baumanni(21/74 ,28 .38% ) ,Klebsiella pneumonia (13/74 ,17 .57% )and Staphylococcus epidermis(12/74 , 16 .22% ) .The resistance rate of acinetobacter baumanni toantibacterial drugs was extremely serious ,showing muti‐drug or pan‐drug resistant phenomena .Conclusion Regular monitoring and analyzing the species and change of drug resistance in pathogenic bacteria isolated from CSF have an important significance to guide clinic to rationally use antibacterial drugs .

10.
Article in Chinese | WPRIM | ID: wpr-476042

ABSTRACT

Objective To investigate the situation of Pseudomonas aeruginosa strains carrying metallo-β-lactamases and inte-grases in the Second Affiliated Hospital of Harbin Medical University.Methods The phenotype of metallo-β-lactamases were detected by modified Hodge test,double-disc synergy and combination paper method,respectvily.PCR method was used to detecte metallo-β-lactamases genotypes and the integrationⅠ,Ⅱ and Ⅲ.The PCR products of the whole length bla-IMP gene were purified,sequenced and analyzed by Blast.Results Among 62 Pseudomonas aeruginosa strains,metallo-β-lactamases phenotype of 3 by modified hodge test,4 by double-disc synergy test,4 by combination paper method and were all positive 4 of IMP-1 type metallo-β-lactamases of Pseudomonasaeruginosa strains (10%,4/40)were positives by PCR.Inte-grase Ⅰ of 16(40 %,16/40)strains were positives by PCR method in IMP-resistant Pseudomonasaeruginosa,and integraseⅠ of 22.73 %(5/22)were positives in IMP-sensitive Pseudomonasaeruginosa.No other metallo-β-lactamases,integraseⅡandⅢ were detected in this study.Conclusion IMP-type metallo-β-lactamase existed in IMP-resistant Pseudomonasaerugi-nosa isolates of the Second Affiliated Hospital of Harbin Medical University.Most strains carried integraseⅠ,and other re-sistance mechanisms may be associated with multi-drug resistance,so it is important to prevent the Pseudomonasaeruginosa strains which carried metallo-β-lactamases and integrons widely spread in the hospital.

11.
Journal of Chinese Physician ; (12): 501-505, 2015.
Article in Chinese | WPRIM | ID: wpr-469486

ABSTRACT

Objective To compare the planning quality and volume of organ at risk (OAR) between volumetric-modulated arc therapyv (VMAT) and nine-field dynamic intensity-modulated radiation therapy (IMRT) in radiotherapy for cervical cancer patients,explore the best way to cervical cancer radiotherapy,Methods Selected 20 patients with cervical cancer were divided into 2 groups,10 cases for each group.Cervical cancer patients with no surgery was performed for A group (group A),received the radical radiotherapy,prescription dose gross tumor volume (GTV) 56 Gy,clinical target volume (CTV) 50 Gy.Another group of patients with cervical cancer radical surgery (group B),giving the whole basin lymph node auxiliary radiation therapy,prescription dose CTV 50 Gy.Each cervical cancer patient received VMAT and IMRT program designs,the differences in dose volume histogram (DVH),irradiated volume of organ at risk (OAR),heterogeneity index (HI),conformity index (CI),maximum dose (PTVmax),minimum dose (PTVmin) and mean dose (PTV mean) were compared between two plans in 2 groups.Results Two kinds of radiation technology in target area dosimetry were not statistical difference between two groups (P > 0.05).In endanger organs on the protection of two groups of VMAT planning groups in the small intestine V20 and left femoral head V20 had obvious advantages with statistically significance (P < 0.05).Conclusions Two groups of dosimetry between VMAT and IMRT program design are similar in cervical cancer.Two groups of VMAT planning groups to protect endanger organ slightly better than that of IMRT group,but VMAT planning group shortens treatment time and improves the accuracy and efficacy of treatment.

12.
Article in Chinese | WPRIM | ID: wpr-484573

ABSTRACT

Objective To construct mutant strains of Klebsiella pneumoniae with ampG gene dele-tion by homologous recombination and to evaluate the role of ampG gene in inducing the expression of AmpC enzyme.Methods Polymerase chain reaction ( PCR) was used to amplify the upstream and downstream fragments of ampG gene.The gene splicing by overlap extension PCR ( SOE-PCR) technique was used to construct the fusion fragment , which was then ligated into the temperature sensitive suicide vector pKO 3-km after enzyme digestion as pKO3-km-ΔampG.To achieve allelic exchange , the plasmid pKO3-km-ΔampG was introduced into Kp1 and Kp NTUH-K2044 strains by electroporation .The mutant strains of Klebsiella pneu-moniae with ampG gene deletion were screened out .The plasmid pACYC184-ampCR was introduced into the Kp NTUH-K2044 wild-type strain and its mutant strain with ampG gene deletion to make them harbor the gene encoding AmpC enzyme .The disk diffusion method was used to evaluate the effects of ampG gene on the expression of AmpC enzyme in Klebsiella pneumoniae strains with cefoxitin as the inducer .Results The recombinant plasmid pKO3-km-ΔampG was constructed successfully .The mutant strains of Klebsiella pneu-moniae with ampG gene deletion were constructed as verified by PCR and DNA sequencing .Compared with the Kp1 wild type strain, no AmpC enzyme was produced by the ampG gene knock-out Kp1 strain.The Kp NTUH-K2044 strain could produce AmpC enzyme , while the mutant strain of Kp NTUH-K2044 with ampG gene deletion could not after introduced the pACYC 184-ampCR plasmid .Conclusion The mutant strain of Klebsiella pneumoniae with ampG gene deletion was successfully constructed .The Klebsiella pneumonia strain without the ampG gene could not produce the AmpC enzyme .

13.
Article in Chinese | WPRIM | ID: wpr-291606

ABSTRACT

<p><b>OBJECTIVE</b>To explore the relationship between expression of coiled-coil domain-containing protein 80(CCDC80) and obesity in serum and adipose tissues.</p><p><b>METHODS</b>A cross-sectional survey was conducted in a hospital in Tangshan in September 2010. 100 people including 78 healthy people and 22 with type-2 diabetes were recruited. Another 36 female patients with benign tumor of Obstetrics and Gynecology were also recruited. Demographic characteristics and serum samples were collected from all subjects, basic biochemical indicators were tested. All subjects were divided into 'Normal Weight' and 'Overweight and Obese' according to their BMI (BMI <24.0 kg/m(2); BMI≥24 kg/m(2)). Serum CCDC80 of the 100 subjects was detected by ELISA. Visceral and subcutaneous fat were derived from the other 36 female subjects, and Real-time PCR was used to detect CCDC80 mRNA expression in adipose tissues. Pearson correlation and multiple linear regression were used to analyze the correlation between serum or adipose CCDC80 expression and waist, BMI, and other biochemical indicators.</p><p><b>RESULTS</b>The age of 100 subjects was (52.9±8.4) years old. 44% of them were women (44 cases) and 56% of them were men (56 cases). After dividing them into three groups according to their BMI, covariance analysis were conducted, and age and gender were adjusted. The HDL-C level was significantly different among three groups (F = 10.73, P < 0.001): 'Overweight and obese combined with diabetes' group ((0.90±0.06) mmol/L)< 'Overweight and obese' group ((1.14±0.04) mmol/L) < 'Normal weight' group ((1.28±0.05) mmol/L). The adjusted expression of serum CCDC80 of the 100 subjects was (5.84±0.16) pg/ml, (5.81±0.98) pg/ml among men and (5.97±0.89) pg/ml among women, and there was no significant difference (t = -0.812, P = 0.419) between genders. ANOVA revealed that there was no significant differences of the expression of serum CCDC80 among three groups (F = 1.06, P = 0.351), 'Normal weight' group was (5.84±0.16) pg/ml, 'overweight and obese' group was (6.11±0.14) pg/ml, and 'Overweight and obese combined with diabetes' group was (5.84±0.19) pg/ml. The analysis showed that FBG had a negative correlation with serum CCDC80 (R(b) = -0.223, P = 0.026). Multivariate linear regression had a similar result, with 1 mmol/L increase of serum FBG, serum CCDC80 decreases for 0.24 pg/ml (β = -0.24, 95% CI: -0.21--0.02). There was also a negative correlation between serum CCDC80 and FBG in overweight and obese people (R(a) = -0.368, P = 0.013). Besides, CCDC80 mRNA was detected in both subcutaneous and visceral adipose tissue of 36 cases, the expression level was 0.06±0.02 for subcutaneous fat, was 0.05±0.04 for visceral fat, and the expression in visceral fat was lower (0.05±0.03) than that in subcutaneous fat (0.06±0.03) (t = 2.50, P = 0.025) among overweight and obese group. There was a negative correlation between waist and visceral CCDC80 mRNA expression (r = -0.472, P = 0.035).</p><p><b>CONCLUSION</b>This study suggested that CCDC80 may be involved in energy and insulin metabolism, and plays a protective role in obesity and diabetes.</p>


Subject(s)
Adipose Tissue , Cross-Sectional Studies , Diabetes Mellitus, Type 2 , Female , Glycoproteins , Humans , Intercellular Signaling Peptides and Proteins , Intra-Abdominal Fat , Male , Middle Aged , Obesity , Overweight , Real-Time Polymerase Chain Reaction , Serum , Subcutaneous Fat
14.
Article in Chinese | WPRIM | ID: wpr-570409

ABSTRACT

Objective To study the changes of cerebral function and pathological morphology before and after the antiparasit-ic treatment with albendazole and praziquantel in patients with cerebral cysticercosis. Methods The data of EEG and neuroimag-ing of 412 patients with cerebral cysticercosis were retrospectively analyzed. Results Before the treatment,the mild abnormali-ty,moderate abnormality,and severe abnormality were observed in 40.53%,45.63% and 13.84% of the patients respectively, which mainly showed the diffuse or focal irregular slow waves,or epileptiform discharges found in the abnormal brain waves. CT/MRI manifestation could be divided into six types,including single sacculus type(23.59%),multiple sacculus type(44.42%), encephalitis type(13.59%),coexistence of macrocyst and sacculus type(4.85%),calcification type(2.18%),and mixed type (11.41%). After 3 courses of the treatment,the normal and improved EEGs were observed in 79.85%and 20.15%,respectively. CT/MRI showed the foci being all absorbed(77.18%),being most absorbed(20.63%),and being no changes(20.18%)which were calcified focus. When cerebral cysticercosis were in acute stage(the single and multiple sacculus type,encephalitis type, and macrocyst and sacculus coexistence type),the therapeutic effect was good;while in the mixed type,the therapeutic effect was relatively poor. If cysticercosis were in the calcification stage,the patients only needed the heteropathy. Conclusions In the pa-tients with cerebral cysticercosis,EEGs show the mild to severe abnormalities,and CT/MRI mainly shows the multiple sacculus type. After the treatment,the abnormal EEGs are gradually recovered and the low density foci can be all absorbed,but some calci-fied focus still exist in some patients.

15.
Herald of Medicine ; (12): 1357-1360, 2014.
Article in Chinese | WPRIM | ID: wpr-454548

ABSTRACT

Objective To determine the solubility and apparent oil/ water partition coefficient of sitafloxacin in different solvents. Methods High performance liquid chromatography (HPLC) was used. The column was Dikma Diamonsil C18 (2) (4. 6 mmí250 mm,5 μm). The mobile phase was 0. 05 mol·L-1 KH2 PO4 solution (pH was adjusted with H3 PO4 to 2. 4)-acetonitrile (7030). The column temperature was set at room temperature. The flow rate was 1. 0 mL·min-1 . The detection wavelength was 295 nm and the injection volume was 10 μL. The solubility of sitafloxacin and the apparent oil/ water partition coefficient at pH 2. 0,4. 3,5. 8,6. 6,7. 4,8. 0,10. 0 and 11. 2 were determined. Results The equilibrium solubility of sitafloxacin in water was 0. 44 mg·mL-1 and the apparent oil/ water partition coefficient was 0. 23 (lgP= -0. 64) at (37±2) ℃ . Sitafloxacin has the lowest equilibrium solubility (0. 13 mg·mL-1 ) and the highest apparent oil/ water partition coefficient in pH7. 4 buffer solution system. At pH>10 and pH<5. 8,the solubility of sitafloxacin increased obviously and apparent oil/ water partition coefficient decreased. Conclusion Sitafloxacin is insoluble in water and also poorly soluble in oil,but its solubility could be improved significantly in acidic or alkaline solution.

16.
Article in Chinese | WPRIM | ID: wpr-451108

ABSTRACT

This paper reports the diagnosis and therapy of one case of Dipylidium caninum infection in an infant.

17.
Article in Chinese | WPRIM | ID: wpr-441185

ABSTRACT

The LHX3 and LHX4 LIM-homeodomain transcription factors play essential roles in pituitary gland.Nervous system developments play certain roles in some cancers,leukemia,and deafness disease.Mutations in the genes encoding these regulatory proteins are closely associated with combined hormone deficiency diseases in human and animal models,and related to nervous system defect,tumors,leukemia and deafness.Analysis of these diseases and the biochemical properties of the LHX3 and LHX4 protein will facilitate a better understanding of the pathogenesis of these diseases.LHX3 and LHX4 are reviewed in this paper.

18.
Article in Chinese | WPRIM | ID: wpr-438481

ABSTRACT

Objective To research the safety of intrauterine device retention during perimenopausal period. Methods The form of questionnaire was used to investigate women in perimenopausal period in this district. We selected 300 women using intrauterine device as observing group and 300 using other contraceptive methods. Not using intrauterine device ) as control group. The enrolled subjects were given consultation and health examination and the menstruation state waist and abdomen's pain and the occurrence of gynecological inflammation were compared between two groups. Results There was no statistically significant difference in the menstrual blood volume of women in perimenopausal period between two groups (P>0.05).There was statistically significant difference in the incidence of pain in waist or abdomen in women in perimenopausal period between two groups (P<0.05). The incidence of gynecological inflammation in women in perimenopausal period was 63.33%and 59.67%in the observing group and control group respectively and there was no statistically significant difference between two groups. Conclusion Intrauterine device retention during perimenopausal period has no impact on women' menstruation state and gynecological inflammation,but is relevant with waist and abdomen's pain.

19.
Article in Chinese | WPRIM | ID: wpr-432494

ABSTRACT

Objective To investigate the clinical effect of the dexamethasone combined with 10% calcium gluconate auxiliary hormone oxytocin to prevent postpartum benorrhage.Methods 128 patients were randomly divide into two groups,control group (n =64):intramuscular injection 20 u oxytocin after delivery of the fetus;observation group(n =64):after entering the secind stage of labor,do a quick infusion with a 5% glucose 100 ml + 10% of the calcum gluconate 10ml,intramuscylar injection 20 u oxytocin + dexamethasone 10 mg after deliver of the fetus.The clinical effects were compared in two groups of prevention of postpartum hemorrhage.Results Both the postpartum 2 h bleeding of observation group[(153.2 ±43.1) ml] and the postpartum 24 h bleeding[(419.3 ± 77.8) ml] were lower than that of the control group[(265.2 ± 86.2) ml,(550.6 ± 99.1) ml] (t =2.553,3.869,all P < 0.01) ; The time of second stage and third stage of labor for the observation group [(26.6 ± 11.1) min,(6.8 ± 1.8) min] were lower than that of the control group [(55.4 ± 12.4) min,(19.8 ± 3.2) min] (t =3.452,3.213,all P < 0.01).Conclusion The effect of dexamethasone combined with 10% calcium gluconate auxiliary hormone oxytocin to prevent postpartum hemorrhage is aplus,which can be pronted and applied in clinic.

20.
Article in Chinese | WPRIM | ID: wpr-432351

ABSTRACT

Objective To explore the safety and effectiveness of dexamethasone and calcium gluconate combined with oxytocin to prevent postpartum hemorrhage.Methods 128 maternal patients with full-term delivery were divided the patients into two parts randomly:the control group and the observation group,of which contained three groups(A,B,C).The patients were all given 20 u oxytocin.As to observation,group A:before 10-15min of cutting skin,give 10mg of dexamethasone and 10 ml 10% calcium gluconate rapid intravenous infusion,group B:give 10mg intravenous infusion of dexamethasone after babies were boro,group C:before 10 ~ 15min of cutting skin,give 10mg of dexamethasone and 10ml 10% calcium gluconate rapid intravenous infusion and then give 10mg dexamethasone after babies were born.The control group wasn't given that medicine,and only give them 20 u oxytocin when babies were born.At last,give contrast of total amount of bleeding,ill reflecting of medicine and the other symptom after 24h.Results The amount of bleeding of observation group A,B,C were (277.3 ± 44.5) ml,(286.8 ± 52.7) ml,(301.8 ±60.9) ml,respectively.The amount of bleeding of the control group was (422.9 ± 78.2) ml.The observation group and control group had significant differences(t =-0.699,0.481,-0.407,all P < 0.01),and there was no significant difference between group A,B,and C (P > 0.05).And this two groups had no bad reflection and the other symptoms.Conclusion Dexamethasone and calcium gluconate combined with oxytocin to prevent postpartum hemorrhage has great efficacy and safety.This method must be extended.

SELECTION OF CITATIONS
SEARCH DETAIL