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1.
Article in Chinese | WPRIM | ID: wpr-905438

ABSTRACT

Brain plasticity is adaptability of brain to environment and experience in neural structure and function. Physical activity, such as aerobic exercise, resistance exercise, balance, and Taiji Quan, etc., can improve brain plasticity, involved various brain regions, and the mechanisms of neuronal and molecular pathways.

2.
Article in Chinese | WPRIM | ID: wpr-905392

ABSTRACT

Executive dysfunction is common in stroke patients. Other than the classical assessment tools, there are performance-based assessments for post-stroke executive dysfunction. The related factors include age, infarct region and area, cardiovascular risk factors (hypertension, diabetes, atrial fibrillation and smoking) and education level. In addition to traditional exercise and cognitive rehabilitation, non-invasive brain stimulation technology (transcranial direct current stimulation and transcranial magnetic stimulation), virtual reality and antidepressant have been used more and more for post-stroke executive dysfunction.

3.
Article in Chinese | WPRIM | ID: wpr-777528

ABSTRACT

The study aims to qualitatively and quantitatively analyze phenolic acids and flavonoids in Artemisiae Argyi Folium cultivated in Qichun(Qiai) for the quality control of this genuine regional herbs. UPLC-LTQ-Orbitrap-MS was used for rapid separation and structural identification of the constituents. Samples were separated on an UPLC column(2. 1 mm×100 mm,1. 8 μm) by gradient elution using 0. 1% formic acid and acetonitrile as mobile phases at a flow rate of 0. 4 m L·min-1. By UPLC-LTQ-Orbitrap-MS,16 compounds including phenolic acids and flavonoids were identified by comparison with reference standards or literature data. For quantitative analysis,12 identified compounds were simultaneously determined by UPLC-DAD at wavelengths of 330 nm. The method was validated with respect to linearity,precision,repeatability,stability and recovery. The contents of these compounds were found to differ significantly between the samples from Qichun and other areas. This strategy was novel,effective and straightforward,which provided a potential approach for holistic quality control of Qiai.


Subject(s)
Artemisia , Chemistry , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Flavonoids , Hydroxybenzoates , Mass Spectrometry , Phytochemicals , Plant Leaves , Chemistry
4.
Article in English | WPRIM | ID: wpr-776604

ABSTRACT

OBJECTIVE@#To evaluate the effect of tai chi exercise on cardiac and static lung function for older community-dwelling adults at risk of ischemic stroke.@*METHODS@#A total of 170 older community-dwelling adults (aged 55-75 years old) at risk of ischemic stroke were allocated to either tai chi training group (85 cases, five 60-min sessions of tai chi training per week for 12 weeks) or control group (85 cases, usual pbysical activity for 12 weeks) using a computer-generated randomization. The echocardiographic parameters of cardiac structure, cardiac function and static lung function were measured at baseline, after 12 weeks of intervention and additional 12-week follow-up period by a blinded professional staffmember using a color Doppler ultrasound imaging device or a cardiopulmonary function instrument. The t test and linear mixed model based on the intentionto-treat analysis principle was used to calculate the effect. The adverse effect was observed.@*RESULTS@#Most of echocardiographic parameters on the cardiac structure, cardiac function and static lung function between the tai chi group and control group did not have a significant difference either post 12-week intervention or additional 12-week follow-up period. Only three parameters involving in right ventricular diameter (P=0.024), main pulmonary artery diameter (P=0.002) and vital capacity maximum (P=0.036) were beneficial to be improved in the tai chi group compared to the control group by the analysis of mixed linear model. No adverse effects were found during the intervention period.@*CONCLUSION@#The 12-week tai chi exercise did not have an obvious beneficial effect on cardiac structure, cardiac function and static lung function in older community-dwelling adults at risk of ischemic stroke. The study with a longer duration of intervention should be necessary. (Trial registration No. ChiCTR-TRC-13003601).

5.
Article in Chinese | WPRIM | ID: wpr-773154

ABSTRACT

To study the effects of ellagic acid(EA)on inflammation and oxidative stress in mice with fatty liver disease induced by AKT gene transfection,the 20 female FVB mice were randomly divided into normal control group,model group and ellagic acid administration group(150,300 mg·kg~(-1)·d~(-1))(n=5).EA experimental groups and model group were using a high pressure into the tail vein transfection plasmid AKT.The next day,EA was started to administered continuously for 5 weeks after the AKT gene transfection,while the model group and the normal control group were given the same amount of saline.After the administration,the liver tissue and serum of mice were taken.HE and oil red O staining were using to observe the histopathological changes in liver;liver function to detect the serum and liver tissue as well as MDA and SOD levels;real-time quantitative PCR(RT-qPCR)was used to measure the mR-NA expression of NF-κB and TNF-α;Western blot and immunohistochemistry were used to measure the expression of NF-κB,TNF-αand COX-2 in liver tissue.RESULTS:: show that after AKT gene transfection,the model group had significant increase in the serum levels of AST,ALT,elevated the levels of MDA and decreased the levels of SOD in serum and liver tissue,aggravated histopathology degeneration and Liver inflammation,and significantly higher expression of NF-κB,TNF-α,IL-6,COX-2 and other inflammatory-related factors in liver tissue.EA administration group significant reductions in the serum levels of AST,ALT,and improved in hepatocyte fatty degeneration and liver inflammation,lower the levels of MDA and increased the levels of SOD in serum and liver tissue,and significant reductions in the expression of NF-κB,TNF-α,IL-6 and COX-2 in liver tissue.These results suggest that EA has obvious anti-inflammatory effect and inhibits oxidative stress and EA has a significant therapeutic effecton AKT gene inducing fatty liver,and the mechanism possibly by inhibiting inflammatory factors of NF-κB,TNF-α,IL-6,COX-2 and anti-oxidative stress-related.


Subject(s)
Animals , Ellagic Acid , Pharmacology , Fatty Liver , Drug Therapy , Genetics , Female , Inflammation , Drug Therapy , Mice , Oxidative Stress , Proto-Oncogene Proteins c-akt , Genetics , Random Allocation , Transfection
6.
Article in English | WPRIM | ID: wpr-327201

ABSTRACT

<p><b>OBJECTIVE</b>To explore the association of the platelet-activating factor receptor (PAFR) gene rs5938, rs313152 and rs76744145 polymorphisms with coronary heart disease (CHD) and blood stasis syndrome (BSS) of CHD in Chinese Han population.</p><p><b>METHODS</b>A total of 570 CHD patients (299 with BSS and 271 with non-BSS) and 317 controls were enrolled. The PAFR gene rs5938, rs313152 and rs76744145 polymorphisms were genotyped using the multiplex SNaPshot technology. The statistical analysis was conducted using a multiple variable logistic regression model.</p><p><b>RESULTS</b>Significant differences were detected in the genotypes frequency distributions of the rs5938 (P<0.01), but not the rs313152 (P>0.05), between the controls and CHD patients. Individuals with an rs5938 or rs313152 mutated allele had a low risk for CHD [adjusted odds ratio (aOR)=0.35, 95% confidence interval (CI): 0.23 to 0.56, P<0.01; aOR=0.65, 95% CI: 0.46 to 0.91, P<0.05, respectively]. After the CHD patients were stratified as BSS or non-BSS according to their Chinese medicine patterns, the rs5938 polymorphism mutated alleles had a significant association with a low risk for BSS of CHD (aOR=0.32, 95% CI: 0.18 to 0.57, P<0.01) and non-BSS of CHD (aOR=0.31, 95% CI: 0.17 to 0.55, P<0.01). The rs313152 polymorphism was associated with a low risk for BSS (aOR=0.51, 95% CI: 0.33 to 0.79, P<0.01), but not for non-BSS (aOR=1.22, 95% CI: 0.81 to 1.85, P<0.05). Furthermore, the interaction effect of the rs5938 and rs313152 polymorphisms for BSS of CHD was significantly based on an aOR value associated with the combination of the rs5938 GT genotype with the rs313152 TC genotype of 0.27 (95% CI: 0.1 to 0.7, P<0.01).</p><p><b>CONCLUSION</b>The PAFR gene rs5938 or rs313152 polymorphisms might be a potential biomarker for susceptibility to CHD, especially to BSS of CHD in Chinese Han population.</p>

7.
Article in Chinese | WPRIM | ID: wpr-294367

ABSTRACT

<p><b>OBJECTIVE</b>To systematically assess the efficacy and safety of Rhodiola in treating chronic stable angina pectoris.</p><p><b>METHODS</b>Our group searched the Cochrane library, PubMed, Embase, Chinese biomedical literature database (CBM), VIP database (VIP), Chinese Journal Full-text Database (CNKI) for the literature published in English and Chinese till April 2013. Randomized controlled trials (RCTs) were included on the therapeutic effect of Rhodiola or Rhodiola plus conventional Western medicine in comparison with the conventional Western medicine treatment on stable angina. Data were extracted according the data extraction form. The literature methodological quality was assessed by using the Cochrane handbook, and data analyzed by Rev-Man 5.2 Software for Meta-analysis. The effect indicators of outcomes was expressed by odds ratio (OR) and 95% CI.</p><p><b>RESULTS</b>A total of 7 randomized controlled trials, 662 cases of stable angina pectoris patients met the inclusion criteria and all published in Chinese, without one scientific design and high quality literature. Compared with the conventional Western medicine treatment, combined with oral administration of Rhodiola could improve the efficiency of anti-angina (OR = 2.49, 95% CI: 1.02 - 6.09). Combined with intravenous infusion of Rhodiola could also improve the efficacy of angina pectoris (OR = 4.86, 95% CI: 2.4 - 9.82). Oral administration of Rhodiola couldn't improve ECG efficacy (OR = 1.25, 95% CI: 0.67 - 2.34). Intravenous infusion of Rhodiola could improve the clinical efficacy (OR = 2.94, 95% CI: 1.61 - 5.35). Combined with the conventional treatment, intravenous infusion of Rhodiola could improve the whole blood viscosity (low and high shear rates) and inverse variance (IV) (-1.36 and -0.99, 95% CI: -1.65 - 1.07 and -1.26 - 0.71), but could not reduce serum fibrinogen and D-dimer level. The incidence rate of adverse reactions was higher than that of the conventional treatment combined with Rhodiola (OR = 0.1, 95% CI: 0.02 - 0.51).</p><p><b>CONCLUSIONS</b>On the basis of routine treatment, Rhodiola could further improve patients' symptoms. Combined with intravenous medication, Rhodiola could increase the ECG improvement rate, and reduce adverse reactions. But the methodological quality of included studies was poor, the number of samples was small, and influence factors such as the intervention period was short. This conclusion needs scientific and rational design in a larger sample, multicenter clinical trial to verify.</p>


Subject(s)
Angina, Stable , Drug Therapy , Chronic Disease , Drugs, Chinese Herbal , Therapeutic Uses , Humans , Randomized Controlled Trials as Topic , Rhodiola , Treatment Outcome
8.
Article in Chinese | WPRIM | ID: wpr-265816

ABSTRACT

<p><b>OBJECTIVE</b>To explore Chinese medical syndrome distribution laws in coronary heart disease (CHD) patients of the Han nationality in Fuzhou city.</p><p><b>METHODS</b>A questionnaire on Chinese medical syndrome was carried out in 507 patients with confirmed CHD from different regions of Fuzhou city. The correlation analyses of Chinese medical syndrome distribution laws, the Chinese medical syndrome types and complications, gender, age, the body mass index (BMI) were conducted.</p><p><b>RESULTS</b>Viewed from elements of deficiency in origin or excess in superficiality, blood stasis syndrome was the most often seen syndrome in patients with CHD (accounting for 63.1%), followed by qi deficiency syndrome (accounting for 59.4%) and phlegm turbidity syndrome (accounting for 45.6%). Among syndrome types, qi deficiency blood stasis syndrome was the most often seen syndrome (accounting for 12.2%), followed by qi deficiency, blood stasis and phlegm turbidity syndrome (accounting for 9.1%), and qi deficiency and phlegm turbidity syndrome (accounting for 8.1%). The distribution of various Chinese medical syndrome types showed significant difference in different ages (P<0.05), but no obvious difference was shown in different genders, body mass index, or complications (P>0.05).</p><p><b>CONCLUSIONS</b>Blood stasis, qi deficiency, and phlegm turbidity were the basic pathogeneses of CHD patients of the Han nationality in Fuzhou city. Syndrome with intermingled blood stasis, qi deficiency, and phlegm turbidity was the main Chinese medical syndrome pattern. The combination of syndrome showed certain regularity.</p>


Subject(s)
Adult , Aged , Aged, 80 and over , Asians , China , Epidemiology , Coronary Disease , Diagnosis , Epidemiology , Female , Humans , Male , Medicine, Chinese Traditional , Middle Aged
9.
Acta Pharmaceutica Sinica ; (12): 967-972, 2009.
Article in Chinese | WPRIM | ID: wpr-354610

ABSTRACT

This study aims to investigate the inhibitory effect on proliferation and metastasis of 20-O-(beta-D-glucopyranosyl)-20(S)-protopanaxadiol (IH901) on ECV304 cell line. MTT assay was used to examine the effect of cell proliferation inhibition and the adhesive ability of ECV304 cells to artificial basement membrane. Morphology of cell apoptosis was observed with phase contrast microscope. Apoptosis rate and cell cycle were detected by flow cytometry (FCM). Cell migration was measured by wound healing assay. ELISA kit was used to detect VEGF and bFGF. Caspases were detected by Western blotting. Results indicated that ginseng saponin IH901 can downregulate the expression of growth promoting protein VEGF and bFGF, and upregulate pro apoptosis protein cleaved caspase-9 and cleaved caspase-3. The increase in the apoptotic sub-G1 fraction is in a dose-dependent manner, and cell cycle arrests in the G0/G1 phase was detected by FCM. Morphological examination of IH901-treated samples showed cells with chromatin condensation, cell shrinkage, and all typical characteristics of apoptotic cells. Therefore, IH901 dramatically suppresses cell proliferation and adhesion and migration of ECV304 cell line.


Subject(s)
Cell Adhesion , Cell Line , Cell Movement , Cell Proliferation , Human Umbilical Vein Endothelial Cells , Cell Biology , Humans , Panax , Sapogenins , Pharmacology , Saponins , Pharmacology
10.
Article in Chinese | WPRIM | ID: wpr-270460

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effects of garlic oil combined with resveratrol on the apoptosis and expression of Fas, bcl-2 and bax in human gastric cancer cell line MGC-803.</p><p><b>METHODS</b>The experiment included three groups which were the control group, the combined medicine group 1 (including 25 microg/ml oil garlic and 25 microg/ml resveratrol) and the combined medicine group 2 (including 50 microg/ml oil garlic and 50 microg/ml resveratrol). The apoptosis of cell was examined by DNA gel electrophoresis and flow cytometry for annexin v; the expression of Fas was determined by flow cytometry at the 24th hour after the treatment; the mRNA expression of bcl-2 and Bax gene were measured by RT-PCR method at the 24th ang 48th after treatment, respectively.</p><p><b>RESULTS</b>The garlic oil combined with resveratrol induced cell apoptosis markedly at the 24th after the treatment The protein expression of Fas in the combined medicine groups was 10.59% and 14.16% respectively. As compared with the control group (5.27%), the statistical significance was obvious. The mRNA level of Bax was elevated significantly, however the mRNA expression of bcl-2 was decreased at the 24th and 48th after the treatment.</p><p><b>CONCLUSIONS</b>The garlic oil combined with the resveratrol might obviously induce the apoptosis of gastric cancer cell line MGC-803 which be involved in increasing the expression of Fas protein and bax gene and decreasing the expression of bcl-2 gene at the same time.</p>


Subject(s)
Apoptosis , Genetics , Cell Line, Tumor , Garlic , Gene Expression Regulation, Neoplastic , Genes, bcl-2 , Humans , Plant Oils , Pharmacology , Proto-Oncogene Proteins c-bcl-2 , Genetics , RNA, Messenger , Stilbenes , Pharmacology , Stomach Neoplasms , Genetics , bcl-2-Associated X Protein , Genetics , fas Receptor , Genetics
11.
Chinese Journal of Epidemiology ; (12): 110-112, 2005.
Article in Chinese | WPRIM | ID: wpr-232125

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the long-time effect on allitridum and selenium in preventing cancer of digestive system.</p><p><b>METHODS</b>Persons who were recruited into the intervention group and took allitridum and selenium to prevent gastric cancer in Qixia county of China from 1989-1991 were followed up to 2001 and data of deaths was collected. The long effect on allitridum and selenium in preventing cancer of digestive system was analysed.</p><p><b>RESULTS</b>Data were compared to placebo group five years (1992-1996) after the termination of intervention to have found that the accumulative mortality rate of all cancer, digestive system cancer and gastric cancer had decreased 45.5%, 41.2% and 63.3% in the intervention group respectively. By stratum analysis, accumulative mortality rate of all cancer, digestive system cancer and gastric cancer had decreased 51.5%, 51.5% and 67.7% in males of the intervention group, respectively. Relative risks for males in the intervention group were 0.48, 0.47 and 0.30 times more than the placebo group, respectively. All of them were statistically significant. Relative risks for females in the intervention group were 0.74, 0.92 and 0.70 times more than placebo group. Six to ten years later after the termination of intervention, the accumulative mortality rate and relative risk of all cancers in two groups became similar.</p><p><b>CONCLUSION</b>Allitridum and selenium had the effect of decreasing the incidence risk of digestive cancer with a protective rate more than 50% for five years after the termination of intervention program.</p>


Subject(s)
Adult , Aged , Allyl Compounds , Therapeutic Uses , Antioxidants , Therapeutic Uses , Female , Follow-Up Studies , Gastrointestinal Neoplasms , Humans , Male , Middle Aged , Selenium , Therapeutic Uses , Stomach Neoplasms , Sulfides , Therapeutic Uses
12.
Chinese Journal of Epidemiology ; (12): 583-587, 2005.
Article in Chinese | WPRIM | ID: wpr-331830

ABSTRACT

<p><b>OBJECTIVE</b>To study the prevalence of child-bearing age women with reproductive tract infection (RTI) and related risk factors in the rural area of Shandong so as to develop measurements on prevention.</p><p><b>METHODS</b>Four communities were randomly selected based on economic levels and a baseline investigation on RTI was carried out.</p><p><b>RESULTS</b>A total prevalence rate of RTI was 47.57% with breakdown for four communities-Zhucheng, Zhangqiu, Daiyue and Wudi as 53.91%, 41.49%, 48.37%, 44.88% respectively. Nearly 60% of women at child-bearing age were ignorant on sexually transmitted diseases (STD). Risk factors of RTI would include incorrect perineum cleanout, bad habit of sexual intercourse and low education level of the husbands.</p><p><b>CONCLUSION</b>High prevalence of RTI was associated with low level of education, lack of awareness on STD related hygiene and low rate of seeing doctor for RTI disorder in those women. The results of this study would benefit to the development of intervention measurement of RTI.</p>


Subject(s)
Adult , Age Distribution , Case-Control Studies , China , Epidemiology , Female , Health Knowledge, Attitudes, Practice , Humans , Hygiene , Infections , Epidemiology , Middle Aged , Reproduction , Risk Factors , Rural Population , Spouses , Surveys and Questionnaires , Young Adult
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