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Chinese Journal of Cardiology ; (12): 667-671, 2012.
Article in Chinese | WPRIM | ID: wpr-326446


<p><b>OBJECTIVE</b>To assess the association between left ventricular (LV) twist and untwist with the severity of diastolic dysfunction of high cardiovascular risk population in the community.</p><p><b>METHODS</b>This cross-sectional survey was performed in high cardiovascular risk people with normal left ventricular (LV) ejection fraction in an urban community of Beijing (n = 620). Normal LV diastolic function was defined in 305 subjects, mild diastolic dysfunction in 266 subjects and moderate/severe diastolic dysfunction in 49 subjects. Peak LV twist, peak twist velocity, peak untwist velocity and untwist rate were measured in apical and basal short-axis images using speckle tracking echocardiography.</p><p><b>RESULTS</b>Peak LV twist was similar among subjects with normal diastolic function, mild diastolic dysfunction and moderate/severe diastolic dysfunction. Peak twist velocity [(129.3 ± 45.3)°/s vs. (118.0 ± 36.2)°/s] and untwist velocity [(-132.9 ± 50.4) °/s vs. (-121.2 ± 41.4)°/s] were significantly higher in mild diastolic dysfunction group than in normal diastolic function group (all P < 0.01) and similar between normal diastolic function and moderate/severe diastolic dysfunction group (P > 0.05). Untwist rate of moderate/severe diastolic dysfunction decreased significantly than that of normal diastolic function [(41.9 ± 32.9)°/s vs. (57.7 ± 36.2) °/s, P < 0.01] and mild diastolic dysfunction group [(41.9 ± 32.9)°/s vs. (60.9 ± 39.9) °/s, P < 0.01].</p><p><b>CONCLUSIONS</b>Twist and untwist parameters are increased/preserved in population with normal systolic function and mild diastolic dysfunction and "normalized" or reduced in those with advanced diastolic dysfunction. The maintaining (if not increasing) of LV twist in early diastolic dysfunction might serve as a compensatory mechanism in case of reduced myocardial relaxation in these subjects.</p>

Aged , Cardiovascular Diseases , Diagnostic Imaging , Epidemiology , China , Epidemiology , Cross-Sectional Studies , Diastole , Echocardiography , Methods , Female , Heart Ventricles , Diagnostic Imaging , Humans , Male , Middle Aged , Risk Factors , Ventricular Dysfunction, Left , Diagnostic Imaging , Epidemiology , Ventricular Function, Left
Article in Chinese | WPRIM | ID: wpr-248824


<p><b>OBJECTIVE</b>To evaluate the clinical significance of AFP-L3 in patients with hepatocellular carcinoma.</p><p><b>METHODS</b>Serum AFP-L3 variants were separated by micro centrifugal column, and detected by chemiluminescence.</p><p><b>RESULTS</b>AFP and AFP-L3 levels were higher in patients with hepatocellular carcinoma than those in patients with chronic hepatitis (P<0.001); as a diagnostic target, the sensitivity and specificity of AFP-L3 were 72.3 percent and 97.2 percent, respectively. Eight patients with hepatitis have higher AFP-L3, but none of them were found with carcinoma by CT three months later.</p><p><b>CONCLUSION</b>AFP-L3 is very useful in the diagnosis of patients with hepatocellular carcinoma.</p>

Adult , Carcinoma, Hepatocellular , Blood , Diagnosis , Centrifugation , Female , Humans , Liver Neoplasms , Blood , Diagnosis , Male , Middle Aged , alpha-Fetoproteins
Article in Chinese | WPRIM | ID: wpr-676534


Objective To investigate the clinical and pathologic features of pulmonary sclerosing he- mangioma (PSH).Methods With clinicopathologic date of 21 cases of PSH patients obtained,all speci- mens were stained by immunohistochemical method with a panel of antibodies including CK,synaptophysin, chromogranin,actin,calretinin,F-Ⅷ,CD_(34) and vimentin.Results PSH usually affects female patients.Age ranged from 26 to 70 years old.The tumor cells showed a mixture histological pattern,with the feature of pap- illary and the proliferation of interstitutial monocyte.Immunohistochemical staining revealed that surface of the papillary cells expressed CK,monocyte expressed synaptophysin.PSH should be distinguished from bronchi- oloalveolar carcinoma,carcinoid and inflammatory pseudotumor.Conclusion PSH is a rare and potential malignant behavior tumor,and is different from benign tumor.

Chinese Journal of Oncology ; (12): 612-614, 2004.
Article in Chinese | WPRIM | ID: wpr-254273


<p><b>OBJECTIVE</b>To study the expression and significance of cyclin E, cyclin D1, CDK4 and p27 protein in esophageal squamous cell cancer (ESCC) and their correlation with tumor differentiation and lymph node metastasis.</p><p><b>METHODS</b>Expressions of cyclin E, cyclin D1, CDK4 and p27 protein in 65 patients with ESCC were quantitatively detected by flow cytometry.</p><p><b>RESULTS</b>The expressions of cyclin E, cyclin D1, CDK4 in poorly-differentiated ESCC were higher than those in well-differentiated ESCC (P = 0.0275, 0.0001, 0.0174). The expression of p27 in poorly-differentiated ESCC was lower than that in well-differentiated ESCC (P = 0.0042). There was positive correlation between cyclin E and cyclin D1, cyclin D1 and CDK4, but negative correlation between cyclin D1 and p27. The expressions of all four proteins were not correlated with lymph node metastasis.</p><p><b>CONCLUSION</b>The expressions of cyclin E, cyclin D1, CDK4 and p27 are closely related to tumor differentiation of ESCC. An imbalance between positive and negative control of cell cycling might be critical in the carcinogenesis of esophageal squamous cell cancer.</p>

Adult , Aged , Carcinoma, Squamous Cell , Metabolism , Cell Differentiation , Cyclin D1 , Metabolism , Cyclin E , Metabolism , Cyclin-Dependent Kinase 4 , Metabolism , Cyclin-Dependent Kinase Inhibitor p27 , Metabolism , Cyclins , Metabolism , Esophageal Neoplasms , Metabolism , Pathology , Female , Flow Cytometry , Humans , Lymph Nodes , Pathology , Lymphatic Metastasis , Male , Middle Aged
Article in Chinese | WPRIM | ID: wpr-305235


The study of mammary gland bioreactor is in the ascendant. In order to generate transgenic goats of well-controlled expression of exogenic genes, we constructed a human lactoferrin (hLF) gene targeting vector containing promoter, exon 1, intron1 and some of exon 2 (about 6.1 kb fragment) and exon 6 approximately 9 (about 3.3 kb fragment) of the goat beta-casein gene as well as hLF minigene, neo gene inserted into them and tk gene ligated to the 3' end of the construct. The 9.4 kb goat genomic sequences as homologous arms were initially amplified by PCR with local goat tissue DNA. The expression vector was named pBC-tk-neo-hlf. Then the recombinant plasmid pBC-tk-neo-hlf containing hLF minigene was transfected into mice mammary tumor cell line C127 by liposome, cell clones were selected with G418. After proliferating, the transfected cells were induced with insulin, luteotropic hormone and hydrocortisone. The result of Western-blotting analysis showed that the transfected cells can secrete hLF protein, and the recombinant protein expressed in cultured cell supernatant has the similar molecular weight as the native protein. The expression level detected by ELISA was 0.21 microg/mL. This result indicated that the targeting vector could efficiently direct the expression of hLF in mammary cells,and it confirmed the validity of the constructed vector. At the same time, C127 cell line proved to be useful for evaluating the regulation of a foreign gene expression in mammary gland specific expression vector.

Animals , Caseins , Genetics , Cell Line, Tumor , Goats , Humans , Lactoferrin , Genetics , Mammary Glands, Animal , Cell Biology , Metabolism , Mice , Molecular Weight , Transfection
Article in Chinese | WPRIM | ID: wpr-683524


Objective To investigate the changes in the glutamate transporter EEAC1 mRNA expression and the injured nerve cell apoptosis after rat cerebral ischemia/reperfusion injury is treated with mild hypothermia. Methods The middle cerebral arteries(MCA)of Sprague-Dawley rats were occluded for 30 minutes,and reper- fused for 90 minutes.Using DIG-labeled CRNA probe and TUNEL,the positive rate of glutamate transporter EE- AC1 mRNA expression and apoptotic cell rate were determined in the sham-operated group,the control group and the mild hypothermia group,respectively.Results The positive rate of EEAC1 mRNA expression and apoptotic cell rate were significantly lower in sham-operated group than those in the control group(P