ABSTRACT
ObjectiveChemical components in ethanol extract of Cyclocarya paliurus dried leaves were analyzed by ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). MethodAn Agilent Poroshell 120 EC-C18 column (3.0 mm×100 mm, 2.7 μm) was used with 0.1% formic acid aqueous solution (A)-acetonitrile (B) as the mobile phase for gradient elution (0-26 min,2%-18%B; 26-60 min, 18%-72%B; 60-70 min, 72%-100%B; 70-71 min, 100%-2%B; 71-72 min, 2%B), and the flow rate of 0.4 mL·min-1 and injection volume of 3 μL. The electrospray ionization (ESI) was used in positive and negative modes, and detection range was m/z 50-1 100. The collected data were processed by Agilent MassHunter workstation. According to the retention time and MS information of each compound, combined with existing literature and MS database information, the compounds were identified and analyzed for the fragmentation rule. ResultA total of 52, 55 components were identified in the positive and negative ion modes, respectively. Among them, 14 flavonoids, 3 triterpenoids, 15 organic acids and 20 other compounds were identified under positive ion mode, while 18 flavonoids, 9 triterpenoids, 18 organic acids and 10 other compounds were identified under the negative ion mode. By summarizing the positive and negative ion modes and removing the common compounds, 87 compounds were identified, including 22 flavonoids, 27 organic acids, 11 triterpenoids and 27 other compounds. ConclusionUHPLC-Q-TOF/MS can be used to quickly analyze the chemical constituents in C. paliurus dried leaves. 1-Kestose and 18β-glycyrrhetinic acid and other components related to hypoglycemic activity of this herb are identified for the first time, which can provide reference for clarifying the pharmacodynamic substance basis of C. paliurus dried leaves.
ABSTRACT
ObjectiveBased on ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS), the changes of endogenous markers in rat plasma at the different stage, namely modeling and administration of Shenling Baizhusan (SLBZS), and the mechanism of SLBZS in the treatment of ulcerative colitis (UC) were studied. MethodIn the modeling stage, rats were randomly divided into normal group, spleen deficiency with dampness retention-UC (SDDR-UC) and pure-UC (P-UC) model group. In the administration stage, SLBZS was given to the above two different model groups. After modeling and administration, rat plasma was collected and determined by UPLC-Q-TOF/MS. The mobile phase was 0.1% formic acid aqueous solution (A)-acetonitrile (B) for gradient elution (in positive ion mode:0-2 min, 99%A; 2-9 min, 99%-73%A; 9-10 min, 73%-44%A; 10-13 min, 44%-38%A; 13-19 min, 38%-28%A; 19-21 min, 28%-2%A; 21-23 min, 2%A; 23-25 min, 2%-10%A; 25-27 min, 10%-99%A; in negative ion mode:0-2 min, 85%A; 2-3 min, 85%-65%A; 3-5.5 min, 65%-44%A; 5.5-8 min, 44%-25%A; 8-10 min, 25%-2%A; 10-16 min, 2%-85%A). The electrospray ionization (ESI) temperature was 120 ℃ under the positive and negative ion modes, and the acquisition range was 50-1 000. Partial least squares-discriminant analysis (PLS-DA) was used to analyze the changes of endogenous metabolites in the above two different model rats from the different stage. MetaboAnalyst 5.0 was used to analyze the metabolic pathways of these identified metabolites. ResultSixteen potential biomarkers were screened and identified in the modeling stage, among which 11 potential biomarkers were common in the two model rats, which mainly affected the primary bile acid biosynthesis pathway. Twenty-three potential biomarkers were screened and identified during the administration stage, among which 3 potential biomarkers were shared by the two model rats, and SDDR-UC and P-UC model rats had 11 and 9 potential biomarkers, respectively. It mainly affected 6 pathways such as purine metabolism, pentose phosphate pathway, pyrimidine metabolism, retinol metabolism, primary bile acid biosynthesis and steroid hormone synthesis. ConclusionThe primary bile acid biosynthesis pathway appears in the different stage of modeling and administration of UC, showing a dynamic change process. The therapeutic effect of SLBZS on SDDR-UC rats may be related to inhibiting the expression of nuclear transcription factor -κB (NF-κB) signaling pathway, activating farnesoid X receptor (FXR) and enhancing the expression of cytochrome P450.
ABSTRACT
OBJECTIVE:To study the ef fects of Gegen qinlian decoction (GGQLD)on blood lipid and blood glucose of hyperlipidemia(HLP)model rats ,and to explore its mechanism from the perspective of intestinal flora. METHODS :Totally 48 rats were randomly divided into blank control group (n=8)and modeling group (n=40). For consecutive 5 weeks,model group was given high-lipid diet to induce HLP model ;blank control group was given routine diet. After modeling ,30 modeling rats were randomly divided into model group ,simvastatin group (positive control ,10 mg/kg),GGQLD high-dose ,medium-dose and low-dose groups (14.85,4.95,1.65 g/kg,by crude drug ),with 6 rats in each group. Blank control group and model group were given constant volume of normal saline intragastrically ;administration groups were given relevant medicine intragastrically ,once a day,for consecutive 11 weeks. At the same time ,each group was continuously given corresponding diet. After the last medication , body mass and body length of rats were determined ,and Lee ’s index was calculated. Serum levels of TG ,TC,HDL-C,LDL-C and fasting blood glucose (FBG)were determined in rats. DNA of rat caecum content was extracted for 16S rRNA V 3-V4 region sequencing. The Two-part model was used to analyze the correlation between intestinal flora with lipids and blood glucose. RESULTS:After 11 weeks of administration ,compared with blank control group ,the body mass ,body length ,Lee’s index , serum levels of TC ,TG,HDL-C and FBG of model group were increased significantly (P<0.05 or P<0.01),while the level of HDL-C was decreased significantly (P<0.05). Compared with model group ,body mass and Lee ’s index and serum levels of TG , FBG of rats in GGQLD high-dose group ,and serum levels of TC ,TG in GGQLD medium-dose group ,as well as serum level of TG of rats in GGQLD low-dose group was decreased significantly (P<0.05 or P<0.01). Correlation analysis with intestinal flora showed that TC and TG shared 3 operational taxonomic units (OTU),including OTU 559,OTU701 and OTU 135(OTU135 was also shared with FBG ),which were all positively correlated with the level of TC ,TG and FBG (P<0.01). The three OTU were annotated as Tyzzerella of Spirillaceae ,Anaerotruncus of Verrucaceae and Peptoclostridium of Streptococcidae ,respectively. High-dose and low-dose GGQLD had a down-regulating effect on Tyzzerella and Anaerotruncus(P<0.05 or P<0.01),while had up-regulating effect on Peptoclostridium(P<0.01). CONCLUSIONS :High-dose GGQLD (14.85 g/kg)can effectively reduce the body mass and blood lipid of HLP model rats ,and can prevent the abnormal increase of blood glucose of model rats. The mechanism may be associated with that the reduction of intestinal flora (Tyzzerella,Anaerotruncus)content.
ABSTRACT
Objective@#To study the effect of activated carbon on the purification of formaldehyde in the clean workshop return air purification device and its influencing factors.@*Methods@#From May to June 2018, choosed 4 different commercial activated carbons (bamboo charcoal, 1-3 mm, 3-5 mm; coconut shell charcoal, 6-12 mesh, 8-16 mesh) to make 5 types of activated carbon purification net. In the simulated clean plant laboratory, the detection of occupational disease hazards was used to test the purification effect of different types of activated carbon purification nets on formaldehyde.@*Results@#The purification effect of different types of activated carbon increased with the prolongation of purification time, and the difference was statistically significant (P<0.05) . Compared with other types of activated carbon, coconut shell charcoal (8-16 mesh, double layer) had the best purification effect, 15 min and 30 min purification efficiency was 58.72% and 85.20% respectively, and the difference was statistically significant (P<0.05) . The purification effect of double-layer coconut shell charcoal was better than single layer (P<0.05) . The purification effect of double-layer coconut shell charcoal (8-16 mesh) was better than double-layer coconut shell charcoal (6-12 mesh) , the difference was statistically significant (P<0.05) . Coconut shell charcoal (8-16 mesh, double layer) had better purification effect than bamboo charcoal (P<0.05) .@*Conclusion@#Different specific surface area, particle size, and thickness of activated carbon have a certain effect on the purification effect of formaldehyde, and its selection has a certain significance in improving the occupational health protection level in the clean plant, solving the safe use of return air and reducing energy consumption.
ABSTRACT
Objective:To explore the curative effects and securities of ticagrelor and clopidogrel in the antiplatelet therapy for coronary heart disease in the aged patients, and to provide a more rational strategy for antiplatelet therapy.Methods:Computer search was performed in Cochrane Central Register of Controlled Trial, Ovid-Medline, EMBase, PubMed, CNKI, Wanfang, VIP and CBM, and the randomized controlled trial (RCT) of ticagrelor and clopidogrel in the treatment of coronary heart disease in the old people were collected.The patients in ticagrelor group were given ticagrelor and other drugs, and the patients in clopidogrel group were given clopidogrel and other drugs.RevMan 5.3software was used to perform Meta-analysis, and the adverse events related to the effectiveness such as the major adverse cardiovascular events (MACE) , myocardial infarction (MI) , stent thrombosis, death, stroke and the adverse events related to safety (bleeding event) were observed.Results:According to inclusion and exclusion criteria, a total of 15RCT were included.The results of Meta-analysis showed that the incidences of MACE (RR=0.59, 95%CI:0.46-0.76, Z=4.08, P<0.01) , MI (RR=0.48, 95%CI:0.28-0.81, Z=2.74, P=0.006) , stent thrombosis (RR=0.16, 95%CI:0.06-0.48, Z=3.30, P=0.0010) , and death (RR=0.52, 95%CI:0.30-0.89, Z=2.41, P=0.02) in ticagrelor group were decreased compared with clopidogrel group;there was no significant difference in stroke rate (RR=0.76, 95%CI:0.39-1.47, Z=0.81, P=0.42) between two groups, and the incidence of bleeding event in ticagrelor group was increased compared with clopidogrel group (RR=1.57, 95%CI:1.20-2.05, Z=3.28, P=0.001) .Conclusion:For the aged patients with coronary heart disease, ticagrelor is superior to clopidogrel;but the incidence of bleeding is increased.
ABSTRACT
This paper was aimed to study the effects of Huang-Lian Jie-Du decoction (HJD) on contents of oxidized low density lipoprotein (Ox-LDL),monocyte chemoattractant protein (MCP-1),vascular cell adhesion molecule (VCAM-1),malondialdehyde (MDA),and superoxide dismutase (SOD) of atherosclerosis (AS) rats' liver homogenate.Male SD rats were randomly divided into six groups,which were the normal control group,model group,positive control group (lovastatin),and the low-,middle-,high-dose HJD groups,with ten rats in each group.Except for the normal control group fed with normal diet,others were fed with high-fat diet,and regularly injected the vitamin D3.All groups were gavaged once daily from the 3rd week for 8 weeks until they were sacrificed.The results showed that compared with the normal control group,contents of MCP-1,VCAM-1 and Ox-LDL in the liver homogenate of the model group had significantly increased (p < 0.05),after drug administration,all indexes mentioned above in the positive control group,the low-and middle-dose HJD group were decreased significantly (p < 0.05);contents of MCP-1 and VCAM-1 of the high-dose HJD group decreased obviously (p < 0.01);MDA content of liver homogenate increased significantly,after drug administration,contents of the positive control group and the low-dose HJD group decreased significantly (p < 0.05);SOD content decreased significantly,after drug administration,contents of both the positive control group and the high-dose group increased significantly (p < 0.05).It was concluded that HJD may play a role in AS intervention.Its mechanism may be related to its anti-inflammatory and antioxidant reaction.
ABSTRACT
Objective:To compare the effectiveness and safety of Dengzhanhuasu and Danshen Injection in the treatment of the patients with angina pectoris of coronary heart disease with Meta-analysis, and to provide basis for their application in clinic.Methods:Computer search was performed using Cochrane Central Register of Controlled Trial,Ovid-medline,EMBASE,PubMed,CNKI,Wanfang,VIP and CBM,and the literatures about the effectiveness and safety of Dengzhanhuasu and Dansheng Injection in the treatment of the patients with angina pectonis of coronary heart disease were obtained.According to the principle of selection and elimination, the literatures were selected, and the qualities of literatures were evaluated.The related data were extracted from the literatures.RevMan 5.3 software was used to extract the data for Meta-analysis.Results:A total of 167 literatures were retrieved, and 8 randomlized-controlled studies were selected according to the selection criteria.The evaluation results of the effectiveness of angina pectoris according to symptom showed that χ2=2.62,df=7,P=0.92,Z=6.85 (P<0.000 01), 95%CI:2.73-6.10. The evaluation of the effectiveness of angina pectoris according to electrocardiogram,χ2=6.37,df=7,P=0.50,Z=6.37(P=0.000 01),95%CI:1.94-3.49.The results of Meta-analysis showed that there were significant differences in the relief of symptoms and improvement of the electrocardiogram efficacy between Dengzhanhuasu and Danshen Injection in the treatment of angina pectoris of coronary heart disease (P<0.05).Conclusion:Dengzhanhuasu has better effectiveness in the treatment of the patients with unstable angina than Dansheng Injection and they have the same safety.
ABSTRACT
This study aimed at investigating the dose-effect relationship of SFI between the blood viscosity and the early-and mid-stage cardiogenic shock and the mediatory effect on rats.The end or root of left anterior descending coronary arteries (LADCA) was ligatured to establish the rat model of the early-and mid-stage cardiogenic shock.The blood viscosity indexes included low shear rate (LSR,10/s),middle shear rate (MSR,60/s),high shear rate (HSR,150/s) of the whole blood viscosity and plasma viscosity (PV),being observed 60 mins after the venous administration of 0.10,0.33,1.00,3.30,10.00 and 20.00 mL·kg-1 SFI (low dosage range:0.1-1.0 mL·kg-1,middle dosage range:1.0-10 mL·kg-1,high dosage range:10-20 mL·kg-1) with a blood rheometer.Dose-response curves were fitted by GraphPad Prism 6.0 software,the dose-response relationship of SFI between the blood viscosity and the early-and mid-stage cardiogenic shock in rats was evaluated to calculate the dose threshold parameters of the indexes.It was found that the blood viscosity indexes were improved with the dosage of 10 mL·kg-1 SFI in rats with the early-stage cardiogenic shock,while the dose-response curves of LSR,MSR and HSR at the early stage all presented favorable s shapes.Most of the effective dose range [D]2o-[D]80 and the threshold dose [D]20 were between 3.3 and 6.3 mL· kg-1.The four indexes of blood viscosity were improved with the administration of 10 and 20 mL·kg-1 SFI in mid-stage model rats with favorable s shapes in the dose-response curves.Most of the effective dose range and the threshold dose were in the range of 3.3 to 10.0 mL·kg-1.In conclusion,most of the dose-response curves of blood viscosity indexes in early-and mid-stage cardiogenic shock rats presented favorable s shapes with the threshold dose between 3.3 and 10.0 mL·kg-1,indicating an effective middle dosage range,which was converted into clinical dosage about 37.1 to 112 mL each day.The research provided an experimental basis for clinical medication.
ABSTRACT
Objective To explore the accuracy of endoscopic ultrasonography (EUS) for evaluating T3 esophageal squamous cell carcinoma (ESCC).Methods The retrospective cross-sectional study was conducted.The clinicopathological data of 733 patients diagnosed with T3 ESCC by preoperative EUS who were admitted to the Sun Yat-sen University Cancer Center from January 2003 to December 2015 were collected.All the patients underwent radical resection of ESCC.The postoperative pathological stage as a gold standard,the accuracy,overstaged and understaged rates of clinical staging by preoperative EUS were assessed.Observation indicators:(1) comparison between clinical T staging evaluated by preoperative EUS and postoperative pathological T staging;(2) follow-up and survival situations.Follow-up using outpatient examination and telephone interview was performed to detect patients' diseases and postoperative survival up to December 30,2016.Overall survival time was from operation time to death or last effective follow-up.Measurement data with normal distribution were represented as (x)±s.Measurement data with skewed distribution were described as M (range).Count data were represented as cases and percentage.The survival curve was drawn by the Kaplan-Meier method,and survival analysis was done using the Log-rank test.Results (1) Comparison between clinical T staging evaluated by preoperative EUS and postoperative pathological T staging:all the 733 patients were confirmed as T3 ESCC by preoperative EUS.Postoperative pathological diagnosis showed that 9 patients were detected in pT1b,87 in pT2,630 in pT3 and 7 in pT4a.The accuracy,overstaged and understaged rates of preoperative EUS in evaluating T3 ESCC were 85.95%(630/733),13.10%(96/733) and 0.95%(7/733),respectively.N0,N1,N2 and N3 of postoperative pathological N stage were respectively detected in 329,247,110 and 47 patients.Twenty-seven,323 and 383 patients were in stage Ⅰ,Ⅱ and Ⅲ of TNM stage,respectively.The high-,moderate-and lowdifferentiated tumors were respectively detected in 125,403 and 205 patients.(2) Follow-up and survival situations:among 733 patients,639 were followed up for 1.0-153.0 months,with a median time of 29.0 months.The median survival time,1-,3-,5-year overall survival rates were 53.0 months (range,37.7-68.3 months),85.3%,58.1% and 48.2% in 733 patients,respectively.The 5-year overall survival rate was 75.2% in 9 patients with pT1b,63.0% in 87 patients with pT2,46.3% in 630 patients with pT3 and 0 in 7 patients with pT4a,respectively,with a statistically significant difference (x2=24.089,P<0.05).Conclusion There is a higher accuracy of EUS for evaluating T3 ESCC,however,the stage migration should be noted.
ABSTRACT
<p><b>OBJECTIVE</b>To study the change of endogenous metabolites of SD rats administrated of aqueous extract of Evodiae rutaecarpa.</p><p><b>METHOD</b>Six SD rats had been successively administrated aqueous extract of E. rutaecarpa (0.3857 g x kg(-1)) for 33 days. An agilent 1200 6410 triplequadrupole mass spectrometer was used for the analysis of endogenous metabolites in rat urine samples. These data was analyzed by the principal component analysis (PCA) and PLS-DA using the SIMCA-P 10.0 software.</p><p><b>RESULT</b>The significant difference in metabolic profiles between the control group and the dosed group was well observed by PCA of the MS data.</p><p><b>CONCLUSION</b>The E. rulaecarpa has changed the endogenous metabolites of SD rats. This work can provide the base for the further research on the interpretation of drug property of E. rulaecarpa.</p>
Subject(s)
Animals , Female , Male , Rats , Evodia , Chemistry , Gene Expression , Metabolomics , Methods , Plant Extracts , Pharmacology , Principal Component Analysis , Rats, Sprague-DawleyABSTRACT
<p><b>OBJECTIVE</b>HPLC-MS/MS-based metabonomics method was used to find the possible biomarker of Rhizoma Coptidis in rat urine.</p><p><b>METHOD</b>Sprague-Dawley rats were successively administrated 7 g x kg(-1) aqueous extract of Rhizoma Coptidis for 30 days, urine were collected by metabolism cages and detected by using the HPLC-MS-MS. All dates were analyzed by the principal component analysis (PCA) through using the SIMCA-P 10.0 software.</p><p><b>RESULT</b>The PCA demonstrated that the metabolome between treated group and control group had difference in rat urine sample after of 22 days administrated, for treated group 169 kinds of biomarkers were found including oxalacetic acid, malic acid, 2-ketoglutaric acid, NE, arachidonic acid, 5-HIAA and other compounds, the result was consistent with pharmacological effects of R. coptidis, such as antiinflammatory, inhibiting biosynthesis of CA biosynthesis, anticentral nerve and energy metabolism inhibition.</p><p><b>CONCLUSION</b>Metabonomics may be available in pharmacological action evaluation of drugs.</p>
Subject(s)
Animals , Male , Rats , Drugs, Chinese Herbal , Pharmacology , Metabolomics , Random Allocation , Rats, Sprague-Dawley , Urine , ChemistryABSTRACT
<p><b>BACKGROUND</b>Pathology is the "gold standard" of the diagnosis of lung cancer, but at present there are few articles about evaluating the value of cytopathological check. The aim of this study is to evaluate the value of cytopathological check in the diagnosis of primary bronchogenic carcinoma of the lung.</p><p><b>METHODS</b>A total of 552 samples' cytopathological results of 248 patients from January 2003 to May 2005 in this hospital were retrospectively analyzed. The samples included pleural fluids (110 for 68 patients), materials from lung puncture (33 for 31 patients), smears of transcatheter bronchial brushing (152 for 138 patients), bronchoalveolar lavage fluids (30 for 26 patients) and sputa (227 for 118 patients).</p><p><b>RESULTS</b>The positive results of different specimens were as follow: the pleural fluids was 69.12%, the materials from lung puncture 67.74%, the smears of transcatheter bronchial brushing 65.22%, the bronchoalveolar lavage fluids 23.08% and sputa 21.19% respectively. The positive rates of the pleural fluids, the materials from lung puncture and the smears of transcatheter bronchial brushing were higher than that of the bronchoalveolar lavage fluids and sputa (P < 0.01). There was no significant difference among the positive rates of the pleural fluids, the materials from lung puncture and the smears of transcatheter bronchial brushing, and between the positive rates of the bronchoalveolar lavage fluids and sputa (P > 0.05).</p><p><b>CONCLUSIONS</b>The cytopathological examination is helpful for the diagnosis of primary bronchogenic carcinoma of the lung, and is one of the important ways to the diagnosis of primary bronchogenic carcinoma of the lung.</p>
ABSTRACT
Objective To evaluate the short-term efficacy of the combination treatment of high intensity focused ultrasound (HIFU) with transcatheter chemoembolization or systemic intravenous themotherapy in the treatment of malignant solid tumors. Methods Eighty-four patients with malignant tumors were treated by transcatheter chemoembolization or systemic intravenous themotherapy, and then by HIFU, from July 2001 to December 2002. The clinical symptoms, organ functions (ALT、AST), and imaging findings (ECT, MRI or CT) before and after the combination treatment were compared. Results After the combination treatment, the remission rate of clinical symptoms was 92 5% (62/67). Of 56 patients with liver cancer, levels of ALT and AST reduced to normal in 58 9% (33/56) and 66 1% (37/56) of the patients, respectively. An AFP decrease ≥50% of originally numeric value was observed in 71 9% of the patients (23/32). MRI or CT findings indicated that the volume of tumor necrosis was ≥50% in 91 7% of patients (77/84), ≥80% in 61 9% of patients (52/84), and
ABSTRACT
Objective To set up the technology for adsorbing and separating total flavone in Sarcandra glabra with macroporous adsorption resin,11 types of macroporous adsorption resins were investigated.Methods The static adsorption and separation were used in investigation of macroporous adsorption resin;the dynamic adsorption and separation were used in studying the condition of adsorption and separation.The total flavone adsorption capacity,total flavone content,and total flavone recovery rate were used as the evaluation criteria.The UV spectrophotometric method was used in the determination of total flavone content.Results The results showed that among 11 types of macroporous adsorption resins,the HPD400 was the best for adsorbing and separating the total flavone in S.glabra in the following technological condition: the concentration of S.glabra sample extract was 10 mg/mL;the maximum adsorbing capacity for total flavone in S.glabra was 9.5 mg/mL;the current velocity was 2.5 mL/min;the eluting reagent was 70% ethanol(three times as the volume of the resin);and the HPD400 resin could be used three times,repeatedly.Conclusion It is a simple and efficient to separate the total flavone in S.glabra under the technological conditions,and a total flavone recovery rate is about 85%.
ABSTRACT
AIM: To compare the fingerprints difference between total flavonoids and coumarins of Sarcandra glabra extract separated by macroporous adsorption resin. METHODS: To establish HPLC fingerprint chromatogram of Sarcandra glabra extract and to evaluate the constituents. RESULTS: The difference fingerprint was showed in total flavonoid part and coumarin part. CONCLUSION: Drug action part could be separated by macroporous adsorption resin and exosyndromed by Spectrogram fingerprints.