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China Pharmacy ; (12): 468-472, 2020.
Article in Chinese | WPRIM | ID: wpr-817294


OBJECTIVE:To establish the method for content determination of 17 kinds of amino acids in Sargassum and its adulterants,and to carry out cluster analysis ,so as to provide reference for quality control of Sargassum. METHODS :Totally of 18 batches of sample (S1-S6 as certified product ,S7-S18 as adulterants )were collected. After acid hydrolysis ,amino acids contents were detected by using automatic amino acid analyzer. The separation was performed on LCAK 06/Na sulfonic acid cation exchange resin column with mobile phase consisted of buffer-regeneration system (gradient elution )at the flow rate of 0.45 mL/min (elution pump )and 0.25 mL/min(derivative pump ). The detection wavelengths were set at 440 nm(proline)and 570 nm(other amino acids ),and the sample size volume was 50 μL. PASW Statistics 18.0 software was used ,and cluster analysis was conducted by using group connection method of cluster analysis with “square Euclidean distance ”as the measurement standard. RESULTS :17 kinds of amino acids were well separated without interference from blank sample. The linear relationship between mass concentration and peak area was good (all r were over 0.998),and the upper and lower limits of the linear range were 48.06 μg/L (cystine)and 1.501 μg/L(glycine),respectively;RSDs of precision ,reproducibility and stability tests were lower than 2%. The average recoveries were between 90.60%-101.56%(RSDs were 0.88%-2.15%,n=6). 17 kinds of amino acids were detected in Sargassum and its adulterants ,among which the contents of glutamic acid ,aspartic acid ,leucine,alanine,glycine and valine were relatively high . Results of cluster analysis showed that 18 batches of sample were clustered into 4 categories,i.e. S 1-S6 into one category;S7-S9 into one category ;S10-S12,S16-S18 into one category ;S13-S15 into one category ;which was consistent with the identification result of Sargassum and its adulterants . CONCLUSIONS :The method is simple , rapid, accurate and reproducible,and can be used for the quantitative analysis and identification of amino acids in Sargassum and adulterants.

China Pharmacy ; (12): 1380-1385, 2019.
Article in Chinese | WPRIM | ID: wpr-816946


OBJECTIVE: To establish a method for simultaneous determination of 27 kinds of heavy metals and trace elements in Halloysitum album from different origins. METHODS: The sample was dissolved by wet digestion. Using inductively coupled plasma mass spectrometry (ICP-MS), carrier gas was argon and collision gas was helium; plasma gas flow rate was 15.0 L/min;   flow rate of carrier gas was 1.17 L/min and collision gas flow rate was 5.0 mL/min; atomizer was Barbinton, and sampling depth was 8.0 mm; atomizing chamber temperature was 2 ℃; radio frequency power was 1.3 kW; peristaltic pump revolutions was 30 r/min. In full quantitative analysis model, the number of test points was 3, the analysis time was 0.1 s, the repetition was 3 times, clustering analysis was conducted by using PASW Statistics 18.0 software. RESULTS: The linear range of 27 kinds of heavy metals and trace elements were 0-200 μg/L(r≥0.996 5); the quantitative limit was 0.003 41-75.485 μg/L and the detection limit was      0.001 1-24.350 0 μg/L. RSDs of precision, stability and repeatability tests were all less than 7%; average recovery was 72.3%- 129.1% (RSD was 0.9%-9.4%, n=6). The content of Al was 0.01-123 220.20 mg/kg, and Al was the element with the highest content. Li, Na, Mg, K, Ca, V, Mn, Fe, Co, Ni, Zn, Ga, Se, Rb, Sr, Ba and U were the principal components of trace elements and could be used as characteristic elements; 26 batches of Halloysitum Album samples could be grouped into 4 categories. CONCLUSIONS: The established method is simple, fast and highly sensitive, can improve the precision and accuracy of test results, and it is suitable for the determination of heavy metals and trace elements in Halloysitum album.

China Pharmacy ; (12): 1535-1540, 2019.
Article in Chinese | WPRIM | ID: wpr-816920


OBJECTIVE: To provide reference for promoting large-scale, standardized and high-quality planting of Chinese medicinal materials. METHODS: Through the communication by phone with the agriculture bureau of each district and county, the contact with the relevant township government and the field visit investigation during Jun. 2013-Dec. 2018 by Lanzhou institute for food and drug control, variety, area, yield and output value, cultivation techniques and processing methods of Chinese medicinal materials in the planting area of Lanzhou were investigated and statistically analyzed. The advantages and problems were analyzed, and reasonable suggestions for planting Chinese medicinal materials were put forward. RESULTS & CONCLUSIONS: In 2018, artificial planting of Chinese medicinal materials in lanzhou has a certain scale, with 21 artificial planting varieties, a total planting area of about 510 000 mu, an annual output of 170 000 tons, an annual production value of over 1.7 billion yuan. Cultivation techniques mainly include seedling transplanting, mulching or direct seeding, while drying is the main processing method. Artificial planting of Chinese medicinal materials in Lanzhou has a certain scale and prominent characteristic varieties; authenticity is guaranteed, and the combination of Chinese medicinal materials planting and tourism drives economic development. However, there are still some problems, such as a certain distance from the development of industrialization, variety degradation, backward basic research, serious natural disasters. It is suggested to strengthen its propaganda and expand its advantages, at the same time, enhance government support, develop and construct planting bases of Chinese medicinal materials, strengthen the awareness of good agricultural practice (GAP), strengthen scientific research strengeh, explore breeding techniques of fine varieties of Chinese medicinal materials, develop insurance of Chinese medicinal materials, and guarantee the development of planting industry so as to promote large-scale, standardized and high-quality planting of Chinese medicinal materials.