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Objective To understand the epidemic characteristics of heart failure in Guangzhou, and to provide a basis for the prevention and control of heart failure. Methods The information of patients hospitalized for heart failure in Guangzhou from January 1, 2017 to December 31, 2021 was collected, and t test, rank sum test, and χ2 test were used to analyze the differences of central tendency and composition ratio. Results From 2017 to 2021, there were 6 823 hospitalized patients with heart failure in Guangzhou, with 8,716 hospitalizations and 849 deaths. The average age of hospitalization was 78.50 ±10.48 years old. Male patients were younger than female patients (P<0.001), and females died 3.86 years later than males on average (P<0.001). The number of hospitalizations for males (4 251 person times, accounting for 48.77%) was less than that for females (4 465 person times, accounting for 51.23%), and the number of hospitalizations for urban registered residence (7 346 person times, accounting for 84.28%) was much more than that for rural registered residence (1 370 person times, accounting for 15.72%). The number of hospitalizations in the fourth quarter was the largest. The combined rates of coronary heart disease, hypertension, type 2 diabetes, chronic obstructive pulmonary disease and hyperlipidemia were 58.65%, 69.52%, 34.92%, 10.69% and 10.23% respectively. There were differences in age, registered residence, residential area and complications among patients with acute and chronic heart failure (P<0.05). Conclusion The mortality of patients with heart failure in Guangzhou is high, and the distribution characteristics of acute and chronic heart failure is different. Corresponding preventive measures should be taken for different groups to reduce the disease burden and improve the quality of life.
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BACKGROUND@#For the reason that many studies have been inconclusive on the effect of humidity on respiratory disease, we examined the association between absolute humidity and respiratory disease mortality and quantified the mortality burden due to non-optimal absolute humidity in Guangzhou, China.@*METHODS@#Daily respiratory disease mortality including total 42,440 deaths from 1 February 2013 to 31 December 2018 and meteorological data of the same period in Guangzhou City were collected. The distributed lag non-linear model was used to determine the optimal absolute humidity of death and discuss their non-linear lagged effects. Attributable fraction and population attributable mortality were calculated based on the optimal absolute humidity, defined as the minimum mortality absolute humidity.@*RESULTS@#The association between absolute humidity and total respiratory disease mortality showed an M-shaped non-linear curve. In total, 21.57% (95% CI 14.20 ~ 27.75%) of respiratory disease mortality (9154 deaths) was attributable to non-optimum absolute humidity. The attributable fractions due to high absolute humidity were 13.49% (95% CI 9.56 ~ 16.98%), while mortality burden of low absolute humidity were 8.08% (95% CI 0.89 ~ 13.93%), respectively. Extreme dry and moist absolute humidity accounted for total respiratory disease mortality fraction of 0.87% (95% CI - 0.09 ~ 1.58%) and 0.91% (95% CI 0.25 ~ 1.39%), respectively. There was no significant gender and age difference in the burden of attributable risk due to absolute humidity.@*CONCLUSIONS@#Our study showed that both high and low absolute humidity are responsible for considerable respiratory disease mortality burden, the component attributed to the high absolute humidity effect is greater. Our results may have important implications for the development of public health measures to reduce respiratory disease mortality.
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Humans , China/epidemiology , Cities/epidemiology , Climate , Humidity/adverse effects , Models, Theoretical , Nonlinear Dynamics , Respiratory Tract Diseases/mortality , Sensitivity and SpecificityABSTRACT
Objective To establish a non-venous bypass orthotopic liver transplantation model in Bama miniature pigs with high repeatability and stability. Methods Twelve Bama miniature pigs were randomly divided into the donor group (n=6) and recipient group (n=6). Pigs underwent non-venous bypass orthotopic liver transplantation. The time of anhepatic phase during operation was shortened, blood pressure during anhepatic phase was stably maintained, and management of anesthesia and body fluid during operation were strengthened. The operation time, anhepatic phase and survival status of the recipients were observed and recorded. The intraoperative heart rate, mean arterial pressure (MAP) and changes in arterial blood gas analysis were monitored. The perioperative liver function was evaluated. Results Among 6 Bama miniature pigs, 1 died from transplantation failure intraoperatively. The operation time of the remaining 5 pigs was (247±27) min and the time of anhepatic phase was (46±4) min. Three animals survived for more than 2 weeks. Compared with the preanhepatic phase, the heart rate of the animals was significantly faster, MAP was considerably reduced to (46±6) mmHg, blood pH value, base excess (BE) and HCO3- level were all significantly decreased and serum level of K+ was significantly elevated during the anhepatic phase (all P < 0.05). In the neohepatic phase, MAP of Bama miniature pigs was significantly increased, heart rate was dramatically slower.Blood pH value, BE, HCO3- level were significantly increased and serum level of K+ was significantly declined (all P < 0.05). During abdominal closure, MAP, blood gas indexes and serum level of K+ were almost recovered to those in the preanhepatic phase. Compared with preoperative levels, the levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), lactate dehydrogenase(LDH)and alkaline phosphatase(ALP)were significantly increased after operation (all P < 0.05), the change in AST was the most obvious, and it gradually decreased at postoperative 2 d. The level of γ-gutamyl transferase(GGT) did not significantly elevated. The level of total bilirubin (TB) was evidently elevated at postoperative 5 d. Compared with the preoperative levels, the levels of total protein (TP) and albumin (ALB) were significantly decreased after operation (both P < 0.05), and began to gradually increase at postoperative 1 d. Conclusions The non-venous bypass orthotopic liver transplantation model of Bama miniature pig is convenient, with highly reproducible and survival rate, which can be utilized as a standardized liver transplantation model.
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Objective To evaluate the effect of the different Child-Pugh classification on the recurrence and survival of hepatocellular carcinoma (HCC) recipients after liver transplantation. Methods Clinical data of 125 HCC recipients undergoing liver transplantation were retrospectively analyzed. The 3-year disease-free survival (DFS) and overall survival (OS) rates were calculated by Kaplan-Meier survival curve. The independent risk factors probably affecting the recurrence and survival of HCC recipients after liver transplantation were identified by using Cox's proportional hazards regression model. Results The median follow-up time was 25.6 months. The 3-year DFS and OS rates were 68.4% and 65.7% for all patients. The 3-year DFS and OS rates in 113 patients with Child-Pugh class A/B HCC were 68.6% and 66.2%, whereas 66.7% and 65.6% for 12 patients with Child-Pugh class C HCC with no statistical significance (all P>0.05). Cox's proportional hazards regression model demonstrated that vascular invasion (P=0.001)and the number of tumors>3 (P=0.025) were the independent risk factors for the postoperative recurrence of HCC in recipients undergoing liver transplantation. Alpha fetoprotein (AFP)>400μg/L (P=0.035), vascular invasion (P=0.031) and number of tumors>3 (P=0.008) were the independent risk factors affecting the survival of HCC patients. Conclusions The postoperative prognosis does not significantly differ between Child-Pugh class C and A/B HCC patients after liver transplantation. AFP, vascular invasion and number of tumors are the risk factors affecting the clinical prognosis of HCC patients after liver transplantation. Liver transplantation is an efficacious treatment for HCC patients with Child-Pugh class C.
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Objective@#To evaluate the burden and to describe the characteristics of spatial distribution caused by malignant tumors among different administrative areas in Guangzhou from 2010- 2013.@*Methods@#Based on data from the Cancer Registry system and population in Guangzhou in 2010- 2013, disability-adjusted life year (DALY) was assessed on the disease burden of cancer, in accordance with the method used in the Global Burden of Disease study.@*Results@#The crude incidence rates of cancer appeared as 256.22/105 in 2010-2011 and 270.04/105 in 2012-2013, with the crude mortality rates as 143.17/105 and 148.01/105, respectively, in Guangzhou. Cancers caused 606 238.95 DALYs in 2010-2011 and 623 763.80 DALYs in 2012-2013 for both sexes and 37.63 and 37.81 person year per 1 000 persons, with the standardized DALY rates as 34.51‰, 34.00‰ respectively. Three administrative districts (Yuexiu, Haizhu and Liwan) were with the largest disease burden of cancers that accounted for 45% of the DALYs for the whole Conghua district, with liver cancer was the leading cancer on DALYs, and tracheal, bronchus and lung cancer ranked the first in the other districts.@*Conclusions@#In Guangzhou, disease burden caused by cancers was both prominently seen in the newly developed urban area and the old districts. It remains an arduous task to continue programs on control and prevention of cancers in this city.
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Objective: To analyze the effect of colorectal cancer screening in the general population in Guangzhou, and provide evidence for the for development of colorectal cancer screening policy and strategy. Methods: The data of colorectal cancer screening in Guangzhou during 2015- 2016 were collected. The participation, the positive rate of fecal occult blood test, the detection rate of colonoscopy and screening effect of colonoscopy were evaluated. Results: A total of 220 834 residents aged 50-74 years received the screening, and the positive rate of the screening was 16.77% (37 040 cases). Colonoscopy was performed for 7 821 cases (21.12%). Colorectal lesions were found in 4 126 cases (52.76%), of which 614 (7.85%) and 73 (0.93%) and 230 (2.94%) were identified as advanced adenoma, severe dysplasia lesions and colorectal cancers, respectively. The detection rates of all colorectal lesions were higher in men than in women (all P<0.01). The diagnostic rate of early lesion was 87.24%, and 99 early cancer cases were found, accounting for 46.26% of the total cases. The overall screening detection rate of colorectal cancer was 104.15/100 000, higher than the incidence rate (81.18/100 000) in colorectal cancer surveillance (P<0.001), but age group <70 years had higher detection rate, age group ≥70 years had higher incidence rate. Conclusions: The colorectal cancer screening strategy in Guangzhou is effective in the detection of the population at high risk, increase the detection rate of colorectal lesions, early diagnosis rate of precancerous lesions and diagnosis rate of early colorectal cancer. The benefit in those aged ≤69 years was more obvious than that in those aged 70-74 years. It is necessary to improve the compliancy of colorectal cancer screening in population at high risk.
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Aged , Female , Humans , Male , Middle Aged , Adenoma/prevention & control , China/epidemiology , Colonoscopy/statistics & numerical data , Colorectal Neoplasms/prevention & control , Early Detection of Cancer/methods , Immunochemistry , Mass Screening/statistics & numerical data , Occult Blood , Predictive Value of TestsABSTRACT
Objective To analyze the effect of colorectal cancer screening in the general population in Guangzhou,and provide evidence for the for development of colorectal cancer screening policy and strategy.Methods The data of colorectal cancer screening in Guangzhou during 2015-2016 were collected.The participation,the positive rate of fecal occult blood test,the detection rate of colonoscopy and screening effect of colonoscopy were evaluated.Results A total of 220 834 residents aged 50-74 years received the screening,and the positive rate of the screening was 16.77% (37 040 cases).Colonoscopy was performed for 7 821 cases (21.12%).Colorectal lesions were found in 4 126 cases (52.76%),of which 614 (7.85%) and 73 (0.93%) and 230 (2.94%) were identified as advanced adenoma,severe dysplasia lesions and colorectal cancers,respectively.The detection rates of all colorectal lesions were higher in men than in women (all P<0.01).The diagnostic rate of early lesion was 87.24%,and 99 early cancer cases were found,accounting for 46.26% of the total cases.The overall screening detection rate of colorectal cancer was 104.15/100 000,higher than the incidence rate (81.18/100 000) in colorectal cancer surveillance (P<0.001),but age group <70 years had higher detection rate,age group ≥70 years had higher incidence rate.Conclusions The colorectal cancer screening strategy in Guangzhou is effective in the detection of the population at high risk,increase the detection rate of colorectal lesions,early diagnosis rate of precancerous lesions and diagnosis rate of early colorectal cancer.The benefit in those aged ≤69 years was more obvious than that in those aged 70-74 years.It is necessary to improve the compliancy of colorectal cancer screening in population at high risk.
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Objective To analysis of incidence and mortality of prostate cancer in urban Guangzhou during 2000-2011 and discuss the trends.Methods The incidence and mortality rates were calculated by analyzing data obtained from Guangzhou Cancer Registration Center.Standardized rates were calculated using the Segi's World Standard Population.According to the characters of morbidity and mortality,ages were divided into three groups of 0-64,65-74 and ≥ 75 years.The average annual percentage change (AAPC) were calculated by Jointpoint Regression Program 4.0.4.Results Altogether 3 029 new cases of prostate cancer and 964 related deaths were registered between 2000 and 2011.The AAPC was 10.01% (95% CI:6.00%-14.20%) in crude incidence rate and 8.04% (95% CI:6.20%-9.90%) in crude mortality rate,resulting in a total increase of 287.76% (from 4.82 to 18.69 per 100 000 population) in incidence and 142.90% (from 2.41 to 5.86 per 100000 population) over the 12 years.There was no significant difference in both standardized incidence and mortality change.The AAPC was 11.51% (95% CI:6.00%-17.30%) in 0-64 age incidence rate (resulting in increase of 315.87% and 1.99 per 100,000 population),4.19% (95% CI:0.30%-8.20%) in 65-74 age incidence rate (resulting in increase of 92.72% and 45.82 per 100 000 population) and 2.77% (95%CI:-1.00%-6.70%) in ≥75 age incidence rate (resulting in increase of 42.84% and 63.13 per 100 000 population).The age incidence rate increase was the largest in the ≥ 75 age group,but fastest in the younger group.The age mortality trend was only statistically significant in 0-64 age group,AAPC was 6.25% (95% CI:0.30%-12.60%).Conclusions The incidence and mortality rates of Guangzhou increased in a fast rate.The prevention and control of prostate cancer should be strengthened.
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Objective To study the clinical effect of bilateral anterior internal capsulotomy on patients with refractory anxiety disorders.Methods Twenty-one patients with anxiety disorders,failed to pharmacological and cognitive behavior therapies for 5 years and admitted to our hospital from November 2009 to January 2012,were chosen; they received MRI-guided stereotactic bilateral anterior capsulotomy.The coordinates of the lesion target were determined under high resolution MRI directly and confirmed by intra-operative impedance test and high frequency stimulation.The lesions were received radiofrequency at 80 ℃ for 60 seconds.Pre-and post-operative mini-mental state examination (MMSE),self-rating anxiety scale (SAS),Hamilton depression rating scale (HAMD) and Hamilton anxiety rating scale (HAMA) were performed by the same psychiatrists.Results In the 21 patients,8 had anxiety-free,10 had significant improvement,and 3 had no significant improvement.No serious surgery-related complications were noted.Nine patients had mild cognitive deficit and transient dysmnesia only at 1-2 weeks after operation.Personality changes,such as lack of motivation,apathy and indolence,appeared in 4 patients.Psychiatric evaluations demonstrated significant decrease of SAS,HAMD and HAMA scores after operation as compared with those before operation (P<0.05).However,the difference of MMSE scores between pre-and post-operation was not statistically significant (P>0.05).Conclusion MRI guided stereotactic bilateral capsulotomy is precise,safe and much an effective treatment for refractory anxiety disorders,whcih is promising to alleviate the symptoms of anxiety and depression,as well as improving the quality of life.
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Objective To investigate the effect ofstereotactic bilateral internal capsulotomy on patients with refractory depression disorders and thier complications.Methods Twenty-one patients with treatment-resistant depression,admitted to and underwent stereotactic bilateral internal capsulotomy in our hospital from March 2008 to December 2012,were chosen.Their clinical data were retrospectively analyzed; before and one year after operation,Hamilton depression rating scale (HAMD),Hamilton anxiety rating scale (HAMA) and mini-mental state examination (MMSE) were performed by psychiatrist to evaluate the treatment efficacy.Results (1) The total scores of HAMA and HAMD after operation were significantly lower than those before operation (P<0.05),while no obvious difference in the MMSE scores was noted between before and after operation.(2) One year after the operation,6 patients met the criterion of clinical control,8 improved significantly,5 got improvement and 2 did not improve; the total effective rate was 90.5% (19/21).(3) Complications included mild cognitive deficit and transient dysmnesia in 3 patients who all recovered in one week,absence of interest as well as inactiveness in 3 patients,and weight-gain in 2.No serious complications such as cerebral hemorrhage and nerve injuries were noted in all the patients after the operation.Conclusion Stereotactic bilateral capsulotomy is an effective treatment method which can be considered as a compensatory way of treating refractory depression disorders.
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Objective To explore the effect of theta-burst stimulation (TBS) of the motor cortex on the suprahyoid muscles and the mechanism through which the bilateral motor cortex regulates the suprahyoid muscles.Methods Continuous TBS (cTBS) was applied to the left motor cortex followed by intermittent TBS (iTBS) applied to the right motor cortex of 24 healthy subjects.The motor-evoked potentials (MEPs) of the suprahyoid muscles on both sides were recorded before the stimulation and after 15 and 30 minutes.The MEP amplitudes of the left and right suprahyoid muscles were analyzed using repeated measures analysis of variance.Results Before stimulation, the average MEP amplitudes of the left and right suprahyoid muscles were (375.29 ± 176.09) μV and (368.17 ± 149.02) μV respectively, significantly lower than the values after the stimulation.Conclusion iTBS can distinctly enhance the excitability of the right motor cortex controlling the suprahyoid muscles and reverse the inhibition caused by cTBS applied to the left motor cortex.Clarifying the effect of TBS on the excitability of the bilateral motor cortex is important for the rehabilitation of dysphagic stroke survivors.
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Objective:To explore the alteration of brain‐derived neurotrophic factor (BDNF) signaling and the influ‐ence of irbesartan on it in hippocampus of angiotensin‐converting enzyme 2 (ACE2) knock‐out (KO) mice . Meth‐ods:The 10~11‐week ACE2 KO (Ace2/y ) mice received daily treatment with angiotensin II (Ang II) type 1 (AT1) receptor blocker irbesartan (50 mg/kg) or placebo for two weeks. The wild‐type mice (WT ,Ace2+ /y ) were regarded as normal control. Western blotting method was used to measure levels of BDNF and extracellular signal regulated kinase 1/2 (ERK1/2) in the mice hippocampus. Radioimmunoassay was used to measure plasma Ang level in mice . Results :Compared with normal WT control mice ,there were significant down‐regulations of BDNF protein expres‐sion [ (1 ± 0.16) vs .(0.54 ± 0.16)] in hippocampus and plasma Ang‐ (1‐7) level [ (55.6 ± 7.5) pg/ml vs .(42.8 ± 5.8) pg/ml] ,and significant rise in ERK1/2 phosphorylation [ (1 ± 0.28) vs .(1.79 ± 0.29)] in ACE2 KO mice (P<0.01 all). After irbesartan treatment ,there were significant rise in BDNF protein expression (0.88 ± 0.13) in hippocampus and plasma Ang‐ (1‐7) level [(59.4 ± 8.4) pg/ml] ,and significant reduction in ERK1/2 phosphoryla‐tion level (1.33 ± 0.19) in ACE2 KO mice (P<0.05 or <0.01) .Conclusion:There are BDNF protein expression down‐regulation and enhanced ERK1/2 phosphorylation in hippocampus of ACE2 KO mice. AT1 receptor blockade irbesartan can improve Ang‐ (1‐7 ) level and hippocampus BDNF expression , while reducing hippocampus ERK phosphorylation signal in ACE2 KO mice ,suggesting that AT1 receptor blockade possesses certain brain protective effect.
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<p><b>OBJECTIVE</b>To evaluate the impacts of air temperature on years of life lost (YLL) among the residents in Guangzhou and Zhuhai, Guangdong province.</p><p><b>METHODS</b>Daily mortality and meteorology data in Guangzhou and Zhuhai were collected, and distributed lag non-linear model (DLNM) was used to evaluate the cumulative and delayed effects of daily air temperature on YLL of total non-accident mortality. The accumulative effect of air temperature on mortality under the extreme high temperature (0-1 days) and extreme low temperature (0-13 days) situation in Guangzhou and Zhuhai were analyzed respectively.</p><p><b>RESULTS</b>The average YLL was 1 928.0 in Guangzhou and 202.5 in Zhuhai. The exposure-response functions seemed to be non-linear. The hot effect seemed to be acute and reached the peak at the same day, while the cold effect reached the peak at 5(th) days and lasted for about two weeks. Low temperature had stronger gross effect than high temperature had. The cold effect among males was greater than that among females in Guangzhou. The hot/cold effect on YLL was greater in people aged ≥ 65 years than in people aged < 65 years and in people suffering from respiratory disease than in people suffering from cardiovascular disease in both Guangzhou and Zhuhai.</p><p><b>CONCLUSION</b>The effects of high and low temperatures on YLL were obvious, and the impact of low temperature was greater. The elderly and people suffering from respiratory disease or cardiovascular disease are the vulnerable populations.</p>
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Aged , Female , Humans , Male , Middle Aged , Air , Cardiovascular Diseases , Epidemiology , China , Epidemiology , Extreme Cold , Extreme Heat , Mortality, Premature , Nonlinear Dynamics , Respiratory Tract Diseases , Epidemiology , Time FactorsABSTRACT
<p><b>OBJECTIVE</b>To investigate the incidence and trends of thyroid cancer in urban areas of Guangzhou, 2000-2011.</p><p><b>METHODS</b>Total of 2 957 new cases with a coverage population of 45 699 522 person-years diagnosed as thyroid cancer in 2000-2011 in urban areas of Guangzhou were extracted from the population-based data base of Guangzhou cancer registry, among which 649 were males and 2 308 were females with a coverage population of 23 391 743 person-years and 22 307 779 person-years, respectively. The crude incidence, Chinese age-adjusted incidence rate and world age-adjusted incidence rate were calculated. Time trends of different gender and age groups were examined using Joinpoint Regression Program.</p><p><b>RESULTS</b>There were a total of 2 957 cases diagnosed as thyroid cancer during 2000-2011 with a sex ratio of 1: 3.56. World age adjusted incidence rates of thyroid cancer in urban areas of Guangzhou increased dramatically from 2.99/100 000 in 2000 to 9.13/100 000 in 2011 with an increase rate of 204.73%. Annual percentage change (APC) was 10.5% (P < 0.001). The APC in world age adjusted incidence rates of thyroid cancer were 8.6% (P < 0.001) for male and 10.9% (P < 0.001) for female, resulting in a total increase of 169.45% (from 1.32/100 000 to 3.54/100 000) for male and 214.64% for female (from 4.73/100 000 to 14.87/100 000). The mean age of onset of thyroid cancer was significantly different between the males and females. The incidence rate of thyroid cancer in females aged 45-59 group (19.56/100 000) reached the highest one.While in males, it was increasing gradually with the age, the incidence increased from 0.74/100 000 in aged 15-19 to 9.48/10 000 in aged of 85 years above. The APC was significant in 20-59 age groups in both males (12.2%) and females (13.4%) (P < 0.001).</p><p><b>CONCLUSIONS</b>The incidence rate of thyroid cancer increased rapidly in urban areas of Guangzhou. The prevention and treatment of thyroid cancer should be focused on 20-59 age groups.</p>
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Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Asian People , China , Incidence , Thyroid Neoplasms , Urban PopulationABSTRACT
Objective To evaluate the impacts of air temperature on years of life lost(YLL) among the residents in Guangzhou and Zhuhai,Guangdong province. Methods Daily mortality and meteorology data in Guangzhou and Zhuhai were collected,and distributed lag non-linear model (DLNM)was used to evaluate the cumulative and delayed effects of daily air temperature on YLL of total non-accident mortality. The accumulative effect of air temperature on mortality under the extreme high temperature(0-1 days)and extreme low temperature(0-13 days)situation in Guangzhou and Zhuhai were analyzed respectively. Results The average YLL was 1 928.0 in Guangzhou and 202.5 in Zhuhai. The exposure-response functions seemed to be non-linear. The hot effect seemed to be acute and reached the peak at the same day,while the cold effect reached the peak at 5th days and lasted for about two weeks. Low temperature had stronger gross effect than high temperature had. The cold effect among males was greater than that among females in Guangzhou. The hot/cold effect on YLL was greater in people aged ≥65 years than in people aged <65 years and in people suffering from respiratory disease than in people suffering from cardiovascular disease in both Guangzhou and Zhuhai. Conclusion The effects of high and low temperatures on YLL were obvious,and the impact of low temperature was greater. The elderly and people suffering from respiratory disease or cardiovascular disease are the vulnerable populations.
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Objective To evaluate the impacts of air temperature on years of life lost(YLL) among the residents in Guangzhou and Zhuhai,Guangdong province. Methods Daily mortality and meteorology data in Guangzhou and Zhuhai were collected,and distributed lag non-linear model (DLNM)was used to evaluate the cumulative and delayed effects of daily air temperature on YLL of total non-accident mortality. The accumulative effect of air temperature on mortality under the extreme high temperature(0-1 days)and extreme low temperature(0-13 days)situation in Guangzhou and Zhuhai were analyzed respectively. Results The average YLL was 1 928.0 in Guangzhou and 202.5 in Zhuhai. The exposure-response functions seemed to be non-linear. The hot effect seemed to be acute and reached the peak at the same day,while the cold effect reached the peak at 5th days and lasted for about two weeks. Low temperature had stronger gross effect than high temperature had. The cold effect among males was greater than that among females in Guangzhou. The hot/cold effect on YLL was greater in people aged ≥65 years than in people aged <65 years and in people suffering from respiratory disease than in people suffering from cardiovascular disease in both Guangzhou and Zhuhai. Conclusion The effects of high and low temperatures on YLL were obvious,and the impact of low temperature was greater. The elderly and people suffering from respiratory disease or cardiovascular disease are the vulnerable populations.
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In this study,a synthesized quadruple antigenic epitope gene region of the classical swine fever virus (CSFV)E2 glycoprotein was expressed in E.coli to a obtain target protein.This target protein was used as a coating antigen to establish an indirect ELISA for specifically detecting anti-CSFV antibodies in serum samples from pigs.The P/N cut-off value of this assay was 1.92 by receiver operating characteristic curve(ROC)analysis based on 30 negative sera and 80 positive samples.The test gave 97.5% sensitivity and 96.7% specificity compared with the indirect hemagglutination(IHA)test.The inter-assay and intra-assay coefficients of variation (CVs)for 16 sera were both ≤6.8%.No cross-reactivity between the coating antigen and anti-bovine viral diarrhoea virus(BVDV)antibodies was observed.
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The ompA gene of Chlamyia psittaci in cows was amplified by PCR with primers designed based on those reported in GenBank.The amplified ompA gene was inserted into the bacterial plasmid vector pGEX-4T-1 and then transformed into E.coli BL21(DE3) with IPTG induction. The gene was derived from plasmid pMD18-T vector and then sequenced.It was demonstrated that this recombinant fusion protein of approximately 68kD in molecular mass was highly expressed in inclusion body and more pure proteins would be produced after purification.The fusion protein specifically reacted with positive sera of bovine Chlamydia as demonstrated by Western blotting. These results indicate that this recombinant fusion protein shows good reactivity and could be used to develop the diagnostic kit for bovine Chlamydia and genetic engineering vaccine.
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The sequence encoding an E2 main antigen glycoprotein of the C strain of classical swine fever virus (CSFV) was highly expressed in the host cell E. coli BL21-CodonPlus (DE3)-RIL using the pGEX-4T-1 expression vector and the soluble recombinant product was purified with Glutathione Sepharose TM<'4B> by centrifugation. The soluble recombinant protein showed good immune reactions and was confirmed by Western blot using anti-CSFV-specific antibodies. Then an indirect ELISA with the purified E2 protein as the coating antigen was established to detect antibody against CSFV. The result revealed that the optimal concentration of coated antigen was 0.6 μg/well and the optimal dilution of serum was 1:80. The positive cut-off value of this ELISA assay was OD<,tested serum>/OD<,negative serum>≥2.1- The E2-ELISA method was evaluated by comparison with the indirect hemagglutination test (IHAT). When a total of 100 field serum samples were tested the sensitivity and specificity were 90.3% and 94.7% respectively. Specificity analysis showed that there were no cross-reactions between BVD serum and the purified E2 protein in the E2-ELISA.
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100 ?g/L), 36(2.90%)male and twenty four (2.09%)female. BLLs(G) in Baiyuen (suburb) children(60.33 ?g/L) was the highest among three districts, Yuxiu (downtown) was 58.09 ?g/L and Zengcheng (countryside)was 56.72 ?g/L. Linear regressions (stepwise) were taken with geometric BLLs as dependent, 34 items as independents in SPSS 11.5. Fathers with lower diploma, narrower houses, better aired houses, fewer hand washings and sucking fingers were the risk factors for children high BLLs. Conclusion BLLs in suburb children become the highest after unleaded gasoline used in Guangzhou. The housing circumstance and hygienic behavior of children may effect the blood lead level.