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Article in Chinese | WPRIM | ID: wpr-880776


OBJECTIVE@#To investigate the protective effect of arctiin with anti-inflammatory bioactivity against triptolide-induced nephrotoxicity in rats and explore the underlying mechanism.@*METHODS@#Forty SD rats were divided into 4 groups for gastric lavage of normal saline, arctiin (500 mg/kg), triptolide (500 μg/kg), or both arctiin (500 mg/kg) and triptolide (500 μg/kg). Blood samples were collected for analysis of biochemical renal parameters, and the renal tissues were harvested for determining the kidney index and for pathological evaluation with HE staining. In the @*RESULTS@#In SD rats, arctiin significantly antagonized triptolide-induced elevation of BUN, Scr and kidney index (@*CONCLUSIONS@#Arctiin can protect the kidney from triptolide-induced damages in rats possibly through the anti-inflammatory pathway.

Animals , Anti-Inflammatory Agents , Diterpenes/toxicity , Epoxy Compounds/toxicity , Furans , Glucosides , Kidney/drug effects , Phenanthrenes/toxicity , Rats , Rats, Sprague-Dawley
Article in Chinese | WPRIM | ID: wpr-773462


OBJECTIVE@#To explore the effect of the composition ratio on substitution of sulfate group in sulfated exopolysaccharide (EPS) from and how sulfate modification affects the anti-tumor activity of EPS.@*METHODS@#We used a chlorosulfonic acid-pyridine method to modify EPS and analyzed the effect of esterification ratio on the degree of sulfate substitution using barium chloride turbidimetry. The sulfate groups binding with EPS were analyzed with infrared spectrum analysis. CCK-8 assay was used to evaluate the inhibitory effect of EPS sulfate (SEPS) on the proliferation of human colon cancer HCT 116 cells, and annexin V-FITC/PI double staining was used to assess the pro-apoptotic effect of SEPS in the cells.@*RESULTS@#The esterifying agent and EPS at the composition ratios of 1:1 and 2:1 resulted in sulfate substitution of 0.98% (SEPS-1) and 1.18% (SEPS-2), respectively, and the substitution was improved by increasing the ratio of the esterifying agent ( < 0.05). Infrared spectrum analysis showed that the S=O stretching vibration absorption peak of -OSO appeared near 1249 cm, indicating that the sulfate group combined with EPS to form sulfate. CCK-8 assay showed that SEPS-1 produced stronger inhibitory effects on the proliferation of HCT 116 cells than EPS within the concentration range of 0.02-0.10 mg/L ( < 0.05). At the concentrations of 0.04-0.08 mg/L, SEPS-2 showed a lower anti-tumor activity than SEPS-1 ( < 0.05). SEPS-1 also showed stronger pro-apoptotic effect than EPS, and as its concentration increased, SEPS-1 dose-dependently increased the ratio of early apoptotic cells and necrotic cells; the cells treated with 0.06, 0.08 and 0.10 mg/mL SEPS-1 showed early apoptotic rates of 6.38%, 11.8% and 12.5%, and late apoptotic and necrotic rates of 5.26%, 8.04% and 6.80%, respectively.@*CONCLUSIONS@#The composition ratio of the esterifying agent has a direct impact on the degree of substitution of EPS, which can be improved by increasing the ratio of the esterifying agent. Sulfate modification of EPS can enhance its antitumor activity, which, however, is not directly related with the degree of substitution.

Article in Chinese | WPRIM | ID: wpr-691224


<p><b>OBJECTIVE</b>To investigate the protective effect of monoside against triptolide-induced liver injury and explore its molecular mechanism.</p><p><b>METHODS</b>BALB/C mice treated with gastric lavage with triptolide and monoside, either alone or in combination, were examined for changes of hepatic biochemical parameters using the serological method. The growth inhibition rate of HepG2 cells treated with triptolide or monoside or both was assessed with MTT assay, and the cell morphological changes were observed using laser confocal microscopy; the expressions of the target proteins in the antioxidative stress pathway were detected using flow cytometry and Western blotting.</p><p><b>RESULTS</b>In BALB/C mice, gastric lavage of triptolide induced obvious hepatic damage. In HepG2 cells, treatment with triptolide significantly inhibited the cell growth, resulting in a cell viability as low as 72.83% at 24 h; triptolide also induced obvious cell apoptosis and cell nucleus deformation, causing an apoptosis rate of 43.1% in the cells at 24 h. Triptolide significantly reduced the expressions of Nrf2 and HO-1 proteins related with the oxidative stress pathway. Combined treatment with morroniside obviously reversed these changes, resulting in significantly decreased hepatic biochemical parameters and the liver index in BALB/C mice and in significantly lowered cell apoptosis rate, improved cell morphology, and increased Nrf2 and HO-1 protein expressions in HepG2 cells.</p><p><b>CONCLUSIONS</b>Monoside protects against triptolide-induced liver injury possibly by relieving oxidative stress.</p>

Article in Chinese | WPRIM | ID: wpr-566727


Chronic prostatitis has similarity with pyocutaneous disease in pathogenesis oftraditional Chinese medicine and western medicine, pathology ofWestern medicine and signs and symptoms ofdisease, we got quiet cutative effect in treating chronic prostatitis from treatment theory ofpyocutaneous Disease.Based on theory ofdiagnosis and treatment ofpyocutaneous disease, we should synthesize differential diagnosis and treatment according to different stages, conform three methods, focus on eliminating method and strengthening vital qi, pay attention to two-level multi-object, involving macro-level and microlevel, and combination oftraditional and western medicine.The common used ancient prescriptions are Wuwei Xiaodu Yin, Gouju Decoction, Chaihu Shengshi Decoction.The common used empitical formula are Qinshi Simiao San, Qianliexian Xiaodu Fang, Qianlixian Tuodu Fang, Qianliexian Fuzheng Fang, Tongjing Huoxue Tang, except for these formula herbs, there are other common used herbs, such as processed zaoci, baijiangcao, hongteng, lianqiao etc.According to sydrome differentiation at stages ofbeginning, middle, the later, the chronic prostatitis can be divided into acute episode and chronic episode.In clinics, we should pay attention to taking care ofpatient's splee and stomach, increasing compliance ofpatient, inducing details ofdiet and living which will enhance clinical effective .

Article in Chinese | WPRIM | ID: wpr-523598


Objective To explore how to improve the diagnosis and surgical treatment of primary gallbladder carcinoma. Methods The clinical data of 59 patients with primary gallbladder carcinoma underwent operative treatment in our hospital from January 1985 to December 1999 were retrospectively analyzed. Results There were 22 males and 37 females in this series (M:F=1:1.68). 59%(35/59) patients were complicated by gallstone. Right upper quadrant abdominal pain, jaundice and abdominal mass were the major clinical manifestations. 34 cases underwent cholecystectomy with 57%(34/59) resection rate. Pathological examination showed that gallbladder carcinomas accounted for 94%. Postoperative 1-,3-,5-year survival rates were 36%(21/59), 20%(12/59)and 10%(6/59), respectively. The postoperative survival rate of the early stage patients was higher than that of the advanced patients(P