Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 24
Filter
1.
Chinese Journal of Ocular Fundus Diseases ; (6): 22-27, 2023.
Article in Chinese | WPRIM | ID: wpr-995590

ABSTRACT

Objective:To observe and analyze the macular microvascular system changes in unilateral pediatric uveitis (PU) and healthy contralateral eyes.Methods:A cross-sectional case-control study. From January 2019 to July 2021, 21 eyes of 21 patients with PU diagnosed in one eye (PU group), 21 unaffected contralateral eyes (contralateral eye group), and 21 age-matched volunteers with 21 eyes (NC group) during the same period were examined in Peking Union Medical College Hospital. Optical coherence tomography angiography was used to scan the 6 mm × 6 mm fundus macular area in the three groups of selected eyes to measure the vessel density of the superficial capillary plexus (SCP) and deep capillary plexus (DCP) of the retina, the area of the avascular zone (FAZ) in the fovea of the macula, the choroidal thickness under the fovea (SFCT), and the retinal thickness in the fovea of the macula (CRT). The device comes with a software choriocapillary flow measurement tool, which can obtain the macula's choriocapillary density (CCD) with the fovea as the center and the diameter of the annular area of 1.0 mm, 1.5 mm, and 3.0 mm, respectively. They were recorded as CCD-1.0, CCD-1.5, and CCD-3.0. The measurement data of multiple groups were compared by analysis of variance; if the variance of the three groups of data was not uniform, the Kruskal-Wallis test was used. Multiple linear regression analysis was used to evaluate the potentially related factors of CCD.Results:Compared with the contralateral eye group and the NC group, the vessel density of SCP ( H=-13.857,-25.500; P=0.043, P<0.001), DCP ( H=-15.333, -31.595; P=0.007, P<0.001) and CCD-1.0 ( H=-14.000,-16.214; P=0.040, 0.012) of the clinically quiescent PU group were significantly decreased. CRT and FAZ were not statistically different between PU and NC groups ( F=0.955; P=1.000, 0.661). Compared with the NC group, the mean vessel density of SCP and DCP in the contralateral eye group decreased, and the difference in DCP vessel density was statistically significant ( H=-16.262, P=0.004). There was no statistically significant difference between the CCD of two groups ( P=1.000). The SFCT of the PU group was significantly thicker than that of the NC group ( F=5.552, P=0.004), however, difference was not statistically significant from the fellow eye group ( F=5.552, P=0.270). The results of multiple linear regression analysis revealed that the CCD-1.0, CCD-1.5, and CCD-3.0 showed a linear correlation with the area of FAZ ( β=-0.494, -0.527, -0.566; P=0.015, 0.009, 0.010) and CRT ( β=-0.322, -0.466, -0.342; P=0.026, 0.002, 0.028). CCD-1.0 and CCD-1.5 showed a linear correlation with the vessel density of DCP ( β=0.277, 0.275; P=0.047, 0.045). Conclusion:Both retinal and choroidal microvasculature are abnormal in resting eyes with PU, and macular circulation disorders may be present in the unaffected fellow eye.

2.
Chinese Journal of Hematology ; (12): 141-147, 2023.
Article in Chinese | WPRIM | ID: wpr-969690

ABSTRACT

Objective: To investigate the causative factors of renal function in newly diagnosed multiple myeloma (MM) patients with renal inadequacy. Methods: 181 MM patients with renal impairment from August 2007 to October 2021 at Peking Union Medical College Hospital were recruited, whose baseline chronic kidney disease (CKD) stage was 3-5. Statistical analysis was performed based on laboratory tests, treatment regimens, hematological responses, and survival among various renal function efficacy groups. A logistic regression model was employed in multivariate analysis. Results: A total of 181 patients were recruited, and 277 patients with CKD stages 1-2 were chosen as controls. The majority choose the BCD and VRD regimens. The progression-free survival (PFS) (14.0 months vs 24.8 months, P<0.001) and overall survival (OS) (49.2 months vs 79.7 months, P<0.001) of patients with renal impairment was considerably shorter. Hypercalcemia (P=0.013, OR=5.654) , 1q21 amplification (P=0.018, OR=2.876) , and hematological response over a partial response (P=0.001, OR=4.999) were independent predictive factors for renal function response. After treatment, those with improvement in renal function had a longer PFS than those without (15.6 months vs 10.2 months, P=0.074) , but there was no disparity in OS (56.5 months vs 47.3 months, P=0.665) . Conclusion: Hypercalcemia, 1q21 amplification, and hematologic response were independent predictors of the response of renal function in NDMM patients with renal impairment. MM patients with CKD 3-5 at baseline still have worse survival. Improvement in renal function after treatment is attributed to the improvement in PFS.


Subject(s)
Humans , Multiple Myeloma/drug therapy , Bortezomib/therapeutic use , Hypercalcemia , Prognosis , Chromosome Aberrations , Kidney/physiology , Renal Insufficiency, Chronic , Retrospective Studies , Antineoplastic Combined Chemotherapy Protocols
3.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 88-94, 2023.
Article in Chinese | WPRIM | ID: wpr-961687

ABSTRACT

ObjectiveTo investigate the effect of Ganoderma lucidum polysaccharides (GLP) on the proliferation, migration, cycle, and apoptosis of hepatocellular carcinoma SK­HEP­1 and Huh­7 cells and to explore the underlying mechanism. MethodSK-HEP-1 and Huh-7 cells were classified into the blank group and low-, medium-, and high-dose GLP groups (3.5, 7, 14 g·L-1). The proliferation of the cells was examined by cell counting kit-8 (CCK­8) assay, and the migration by scratch assay. Cell cycle was measured by flow cytometry and apoptosis was detected based on Hoechst33258 staining. In addition, the expression of phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), phosphorylated PI3K (p­PI3K), and phosphorylated Akt (p­Akt) in the cells was determined by Western blot. ResultCompared with the blank group, the three doses of GLP reduced the proliferation and migration of SK­HEP­1 and Huh­7 cells (P<0.05), increased the percentage of cells in G1 phase (P<0.05), and decreased percentage of cells in S and G2 phase (P<0.05). In addition, the three doses can induce apoptosis of both SK-HEP-1 and Huh-7 cells, particularly the high dose. Moreover, the three doses of GLP lowered the levels of p­PI3K and p­Akt (P<0.05). ConclusionGLP significantly inhibited the malignant phenotype of SK-HEP-1 and Huh-7 cells through the PI3K/Akt signaling pathway.

4.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 84-90, 2022.
Article in Chinese | WPRIM | ID: wpr-940521

ABSTRACT

ObjectiveTo study the effect-enhancing and toxicity-reducing activities of astragalus polysaccharide injection (APS) on U14 cervical cancer in model mice receiving X-ray treatment. MethodU14 mouse cervical cancer cells were cultured in vitro and injected into the right forelimb armpit of Kunming mice for constructing a subcutaneous tumor-bearing model of cervical cancer. The tumor-bearing mice were randomly divided into the model group, X-ray intervention(IR, 6 Gy) group, APS (10 mL·kg-1·d-1) group, and IR + APS group. Following the observation of the state, body mass, and food intake of mice in each group, the volume of the tumor was measured. The tumor cell cycle and apoptosis were determined by flow cytometry. The protein and mRNA expression levels of apoptosis-related proteins p53, B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), and cleaved cysteine-dependent aspartate-directed protease-3 (Caspase-3) in tumor tissues were assayed by Western blot and real-time fluorescence quantitative polymerase chain reaction (Real-time PCR). ResultThe comparison with the model group showed that mice in the IR group had poor mental status and reduced mobility. The IR group and IR + APS group exhibited reduced food intake and body mass since the 8th d (P<0.05, P<0.01) and narrowed tumor volume since the 9th d (P<0.01). In the IR group, the proportion of cells in the G1 phase was increased, while the proportion of those in the S phase was decreased (P<0.01). In the IR + APS group, the proportion of cells in the G1 phase rose, whereas the proportion of those in the G2 and S phases cells declined (P<0.05, P<0.01). The apoptotic rates in both the IR group and IR + APS group were elevated significantly (P<0.01). Compared with the model group, the IR group and IR + APS group displayed up-regulated cleaved Caspase-3 and Bax protein and mRNA expression in tumor tissues, but down-regulated Bcl-2 and p53 protein and mRNA expression (P<0.05, P<0.01). Compared with the IR group, the mice in the IR + APS group had better mobility and hair, normal body mass, and increased food intake (P<0.05). The tumor volume in the IR + APS group was reduced (P<0.05). The proportion of cells in the G2 phase was reduced, but the proportion of those in the S phase was raised (P<0.05). The apoptosis rate was increased (P<0.05). The apoptosis-related protein Bax protein expression in the tumor tissue was up-regulated, while the protein expression levels of Bcl-2 and p53 were down-regulated (P<0.05, P<0.01). ConclusionAPS maintains the life state of U14 cervical cancer model mice treated with X-ray and promotes tumor cell apoptosis, thus enhancing the efficiency and reducing toxicity.

5.
Acta Pharmaceutica Sinica ; (12): 14-21, 2019.
Article in Chinese | WPRIM | ID: wpr-778665

ABSTRACT

Spironolactone, a class II drug of the biopharmaceutics classification system, has low oral bioavailability due to poor solubility. Spironolactone solid dispersions were prepared using the solvent method in order to improve its aqueous solubility. Optimization studies of spironolactone solid dispersions were performed using in vitro dissolution tests. Differential scanning calorimetry, X-ray diffraction and Fourier transform infrared were used to investigate the physical state of the drug in carrier materials and to detect the possible interactions between the drug and carrier materials in the solid dispersions. In addition, stress tests were employed to elucidate the key factors which have influence on the stability of the spironolactone solid dispersions. Results showed that spironolactone in the solid dispersions formulated with Soluplus and HPMC-E5 were both in amorphous state and the hydrogen bonds between the drug and carrier materials were formed in the solid dispersion. Therefore, the in vitro dissolution of spironolactone was also significantly enhanced. Stress tests demonstrated that the physical stability of spironolactone solid dispersions prepared with Soluplus was greatly improved compared to those formulated with HPMC-E5. Thus, spironolactone solid dispersion formulated with Soluplus using the solvent method could be used to improve the in vitro dissolution and stability of poorly soluble drugs.

6.
Chongqing Medicine ; (36): 329-331, 2015.
Article in Chinese | WPRIM | ID: wpr-462843

ABSTRACT

Objective To investigate the training of recruits′psychological stress and its relevant influencing factors ,so as to of‐fer scientific basis for the psychological training and psychological health education of recruits .Methods Questionnaire survey method was used and a total of 720 training of recruits in 2013 was sampled .The application of the stress response questionnaire (SRQ) ,eysenck personality questionnaire (EPQ) ,trait coping style questionnaire (TCSQ) ,symptom checklist (SCL‐90) were used to carry out the psychological test and analysis on these recruits .Results The total score of the SCL‐90 and the score of each factor were significantly lower than the norm of recruits in year of 2000(P0 .05) .According to what was shown in the related analysis ,the SCL‐90 total score .SRQ total score had significant positive correlation with emotional stability and negative coping style(P<0 .01) ,but had significant negative correlation with introversion‐extroversion and waste score(P<0 .01) ,and the total score of SCL‐90 had significant positive correlation SRQ to‐tal score(P<0 .01) .It was shown in the regression analysis that factors had significant predictive effect on the psychological factors of new soldiers during the training period respectively were as follow :negative coping ,stability ,introversion‐extroversion ,psychoti‐cism and whether they are the only child or not .The decisive coefficient of 5 variables was R2 =0 .558 ,F=163 .65(P<0 .01) .Con‐clusion The overall mental health of 2013 recruits training are in good condition ,individual mental health status ,personality traits and coping style training of recruits psychological stress are significant predictors .

7.
Journal of Experimental Hematology ; (6): 195-201, 2015.
Article in Chinese | WPRIM | ID: wpr-259615

ABSTRACT

<p><b>OBJECTIVE</b>This study was to expand the cytotoxic T lymphocytes (CTL) through inducing the differentiation of umbilical blood monomuclear cells (UBMNC) by using various combination of cytokines, and to investigate the functions of expanded CTL.</p><p><b>METHODS</b>The MNC were isolated by ficoll density gradient centrifugation. Then, the PHA-P, IFN-γ combined with IL-2, IL-15 and other cytokines were used for induction and expansion of the cord blood-derived CTL. The biological function of CTL was examined by phenotype analysis, cytotoxic tests and real-time fluorescence quantitative PCR.</p><p><b>RESULTS</b>After expansion for 15 days, the cell number increased by 1522% ± 137%. The content of CD3(-)CD8(-) cells in uncultured cord blood MNC was 95%, and the CD3(+)CD8(+) CTL cells reached 82.77% in cultured cord blood MNC after expansion for 15 days. The expanded CTL cell showed the cytotoxic activity against K562 and HeLa cell line. The killing rate of MNC was 61.88 ± 1.08%. After expansion, the killing rate could reach to 90% with the average value of 90.33 ± 2.02%. The expanded CTL cells highly expressed some key cytokines, such as granzyme A, granzyme B, GM-CSF, granulysin, IFN-γ, TGF-β, TNF-α and perforin. Compared with the control group, the expression of IFN-γ and TGF-β significantly increased (P < 0.05), and the other factors dramatically increased (P < 0.01).</p><p><b>CONCLUSION</b>The cord blood-derived CTL can be expanded by different combinations of cytokines. These protocols may provide alternative choices for CTL cell expansion in tumor adoptive immunotherapy.</p>


Subject(s)
Humans , Cytokines , Fetal Blood , Granulocyte-Macrophage Colony-Stimulating Factor , Granzymes , Histocompatibility Antigens Class I , Histocompatibility Antigens Class II , Immunotherapy, Adoptive , Perforin , Phytohemagglutinins , T-Lymphocytes, Cytotoxic
8.
Chongqing Medicine ; (36): 3184-3186, 2014.
Article in Chinese | WPRIM | ID: wpr-455972

ABSTRACT

Objective To study the effect of lipide diet on pharmacokinetics of levofloxacin (Lfox) in beagle dogs .Methods In randomized cross over experiment ,6 Beagle dogs were given Lfox 10 mg/kg at the condition of empty stomach or with lipide diet for 2 week .Plasma concentration of Lfox was determined by HPLC at different time (during 48 h) .The pharmacokinetic parameters were calculated by 3p97 software .Results The plasma concentration-time curves were two compartment modelat the condition of empty stomach or lipide diet .Main pharmacokinetic parameters were as follows :t1/2β:(11 .07 ± 8 .46)and(21 .79 ± 4 .59)h ;tmax :(3 .42 ± 0 .41) and(2 .00 ± 0 .32)h;Cmax :(2 .71 ± 0 .60)and(2 .52 ± 0 .38)μg/mL ;AUC0→ t :(36 .51 ± 8 .66) and(37 .19 ± 8 .21)μg · h-1 · mL -1 ,respectively .There were significant differences in pharmacokinetic parameters of tmax (P<0 .01)except for t1/2β、Cmax and AUC0→ t .Conclusion Lipide diet can quicken tmax ,has no effect on the absorption of Lfox .

9.
Chinese Journal of Biochemical Pharmaceutics ; (6): 65-67, 2014.
Article in Chinese | WPRIM | ID: wpr-452135

ABSTRACT

Objective To investigate Anti-aging function of Placenta freeze-dried powder on mice. Method 2 month old female-KM mice were divided into five groups:normal control group, aging model group, positive control group, placenta freeze-dried powder low dose group and high dose group. Except normal control group,the rest of groups were treated with method of subcutaneous continuous injection of D-galactose for 42 days in order to establish mice aging model. Meanwhile, the corresponding drugs, via intragastric administration, were ingested by different treated groups and observe the change of immunity and physiological regulation related in mice. Results Compared with aging model group, thymus index and spleen index in placenta freeze-dried powder low dose group and high dose group increased obviously (P<0.05), content of MDA in brain decreased significantly (P<0.05), proportion of CD 4 and CD 8 lymphocyte in total lymphocyte, female hormone(P and E 2), IgG and haemopoietic factor in peripheral blood increased remarkably. Conclusion Placenta freeze-dried powder could slow the aging process on mice via immunity enhancement and improving physical regulation.

10.
Chongqing Medicine ; (36): 1088-1089,1092, 2014.
Article in Chinese | WPRIM | ID: wpr-598881

ABSTRACT

Objective To study the efficacy and safety of different simvastatin doses on hyperlipidemia in the elderly .Methods 120 patients with hyperlipidemia were randomly divided into two groups ,60 cases in each group ,patients were given simvastatin 10 mg/d(10 mg/d group) or 20 mg/d(20 mg/d group) .Data were collected before and after treatment ,including total cholesterol (TC) ,three acyl glycerin (TG) ,low density lipoprotein cholesterol (LDL-C) ,high density lipoprotein cholesterol (HDL-C) ,treat-ment efficacy and adverse reactions events .Results 3 month after treatment ,TC ,TG ,LDL-C and HDL-C were better than before treatment in both groups(P0 .05) .Conclusion 20 mg/d simvasta-tin is better than 10 mg/d simvastatin in efficacy of elderly patients with hyperlipidemia ,and offers a safe and reliable treatment .

11.
Chongqing Medicine ; (36): 4766-4768, 2014.
Article in Chinese | WPRIM | ID: wpr-457855

ABSTRACT

Objective To compare the curative effect and safety between ziprasidone injection and haloperidol injection in the treatment of acute agitation of schizophrenia .Methods Totally 86 patients with acute agitation of schizophrenia were divided into observational group(n=43)and control group(n=43) ,the study used a random ,single‐blinded and clinical controlled experiment . the observational group was given ziprasidone mesylate 10-20 mg every time by intramuscular injection and the amount was less than 40 mg every day ;control group was given haloperidol injection 5-10 mg every time by intramuscular injection and the amount was less than 30 mg every day .Drugs in the two groups could be repeated according to the state of illness after 4-6 hours ,and the daily injections were no more than 3 times and the course of treatment was 3 days .The Positive and Negative Symptoms Scale Ex‐cited Factor(PANSS‐EC)was used to evaluate the agitated symptoms before treatment and 2 ,6 ,24 ,48 ,72 hours after treatment ,the Positive and Negative Symptoms Scale(PANSS)and Clinical Global Impression Scale‐Severity of Illness(CGI‐SI)was used to evalu‐ate the curative effect ;extrapyramidal side adverse reaction(SAS) ,Treatment Emergent Symptoms Scale(TESS)and the related lab tests were employed to assess the adverse reaction .Results Comparing with the baseline ,PANSS‐EC score of observe group de‐creased significantly at 2 h after the treatment ;at other observation time‐points ,PANSS scores ,PANSS‐EC scores and CGI‐SI scores in the two groups both decreased significantly(P0 .05) .There were no significant difference in the total effective rate between the two groups(P>0 .05) .There were no serious ad‐verse events in the two groups .incidences of adverse reactions of observe group were 37 .21% ,which were significantly lower than that of control group 53 .49% (P<0 .05) .Conclusion Effect of ziprasidone injection and haloperidol injection was comparable in the treating acute agitation of schizophrenia ,which could treat acute agitation of schizophrenia with low incidences and excellent se‐curity .

12.
Chinese Journal of Behavioral Medicine and Brain Science ; (12): 931-934, 2014.
Article in Chinese | WPRIM | ID: wpr-470623

ABSTRACT

Objective To provide references for the plan-making of mental intervention on new recruits through the exploration of the comprehensive psychological behavior training influences on psychological stress and health and relevant factors.Methods 436 new recruits in training period were divided into comprehensive psychological behavior training group (intervention group) and non intervention group (control group).By randomly selected 2 (intervention group,control group) × 2 (Before training and after training) matching design,the questionnaire survey was carried out using stress-reaction questionnaire (SRQ),symptom checklist 90 (SCL-90),eysenck personality questionnaire(EPQ),trait coping style questionaire(TCSQ) at the 15th day and 75th day.t-test between group and matched t-test within group and multiple stepwise regression analysis were carried out.Results (1) The SCL-90 total point of new recruits in intervention group (125.26±36.52)and control group(124.92± 34.79) before the training and the difference of SRQ and TCSQ factor scores had no statistical significance(P> 0.05).The difference after the comprehensive psychological behavior training had no statistical significance (P> 0.05) as the total points of SCL-90 of intervention group(125.63±36.74) and other factors' score were compared with before.Except for the somatization and force,the total points of SCL-90 (131.21 ± 39.98)and other factor scores of the control group were all higher than what they were in the early period of the training significantly (P< 0.01 or P<0.05).In the later period of group training,from the parallel controlled study among groups we found that except for the somatization and force,the total points of SCL-90 and other factor scores of the control group are all lower than the control group (P<0.0 or P<0.05).(2)The total points of intervention group's SCL-90 and SRQ had significant positive correlation with negative coping and stability(r=0.313-0.698,P<0.01) while it had negative correlation with introversion and extroversion(r=-0.293--0.207,P<0.01) as well as dissimulation at the later period of group training,however,it did not have significant correlation with positive coping method (P>0.05).(3) The regression analysis showed that factors significantly affected the total score of SCL-90 respectively was:SRQ total score,negative coping method,stability,introversion-extroversion and psychoticism (P< 0.01).Five variables'coefficient of determination R 2 =0.665,F=247.82,P< 0.01.Conclusion The comprehensive psychological behavior training could reduce the over psychological stress of new recruits,promote their positive coping attitude and protecting their psychological health.Coping method and personality characteristics are important mediating variable between new recruits' psychological stress and mental health.

13.
Tumor ; (12): 1074-1080, 2013.
Article in Chinese | WPRIM | ID: wpr-848894

ABSTRACT

Objective: To evaluate the relationship between obesity and prognosis-related factors in female patients with breast cancer. Methods: The high-quality cohort studies and case-control studies regarding obesity and the prognosis-related factors of breast cancer were identified by searching PubMed, Cochrane Library, Web of Science, China Academic Journals Full-text Database, Chinese Biological Medical Literature Database and Wanfang Data up to the December 2012. The Meta-analysis was conducted using RevMan5.0 software. Results: A total of 2 002 articles related to obesity and the prognostic factors of female breast cancer were searched out. Finally, 8 studies were included in this Meta-analysis by inclusion and exclusion criteria. The Meta-analysis revealed that the rates of lymph node metastasis (odds ratio: 0.89, 95% confidence interval: 0.85-0.93, P 2 cm (odds ratio: 0.60, 95% confidence interval: 0.42-0.85, P = 0.004) and pathological grade 3 tumor (odds ratio: 0.88, 95% confidence interval: 0.79-0.99, P = 0.003) were significantly different between the obesity group and non-obesity group. The subgroup analysis was performed in 6 studies with the same body mass index classification criterion and the prognostic factors. Three studies in English indicated that the rates of estrogen receptor-positive (odds ratio: 0.63, 95% confidence interval: 0.41-0.98, P = 0.04) and estrogen receptor-negative (odds ratio: 1.58, 95% confidence interval: 1.02-2.44, P = 0.04) were both significantly different between the obesity group and non-obesity group, but there were no significant relationships between obesity and the other prognostic factors. Conclusion: Current evidence indicates that obesity plays a negative role in the prognosis of female patients with breast cancer when the body mass index classification criterion is not considered. Obesity may exert different impacts on the prognosis of female patients with breast cancer in different countries or patients of different races when the subgroup analysis is conducted in the studies with the same body mass index classification criterion and the same prognostic factors. Copyright © 2013 by TUMOR.

14.
Chongqing Medicine ; (36): 4292-4293, 2013.
Article in Chinese | WPRIM | ID: wpr-440091

ABSTRACT

Objective To analysis 2 years follow-up clinical effects in endodontic lesion combined periodontal in treatment with Vitapex .Methods 112 patients with periodontal endodontic lesion combined periodontal were randomly divided into control group (56 cases ,75 teeth) and study group(56 cases ,77 teeth) .Treatment of Zinc oxide glycerin paste combined with gutta percha filling root canal was used in control group ,while Vitapex combined with gutta percha filling root canal was used in study group .After 2 years ,the clinical data of the two groups were analysed .Results The total effective rate of anterior and posterior in study group were significant higher than in control group(P<0 .05) .The total effective rate of of I type ,II type and III type lesions in study group were higher than in control group(P<0 .05) .Conclusion Treatment of Vitapex combined with gutta percha filling root canal in endodontic lesion combined periodontal has better clinical effectiveness to promote periapical and periodontal tissue healing than control group .

15.
Journal of Medical Biomechanics ; (6): E124-E128, 2010.
Article in Chinese | WPRIM | ID: wpr-803657

ABSTRACT

Objective To obtain kinematic and kinetic parameters of gait initiation from young men when they are walking with constant speed. MethodDLUT4060 piezoelectric multi component force platform and DVM8820 three dimensional infrared image capture system were used to test gait initiation in 9 young men (average age was (21.3±0.5) years). ResultsThe lower limb kinematic and dynamic characteristics during gait initiation differ significantly from its subsequent steady state. The characteristic feature of gait initiation is the moving leg swinging a larger range and the stance leg performing a more powerful thrust. The center of pressure (COP) in the foot bottom shows a moving track which moved to the lateral of the moving leg firstly and then to the extension of the stance leg. ConclusionsNormal gait initiation has its unique characteristics, and the kinematics and dynamics parameters obtained in such status will be helpful for gait analysis and bio robot design.

16.
China Pharmacy ; (12)2007.
Article in Chinese | WPRIM | ID: wpr-533062

ABSTRACT

OBJECTIVE:To establish a HPLC-chiral stationary phase(CSP) for separating the enantiomers of lactic acid and determining the optical purity of lactic acid.METHODS:Lactic acid samples of different optical purity were determined by HPLC-CSP vs.optical rotation method.The enantiomers of lactic acid were separated on Chirex 3126(D)-penicillamine chiral column.The mobile phase consisted of copper sulfate solution-isopropyl alcohol (85∶15) at a flow rate of 0.6 mL?min-1.The detection wavelength was set at 254 nm.RESULTS:A baseline separation of the enantiomers of lactic acid was achieved with a separation factor of greater than 1.2.The calibration curve was linear in the range of 0.75~48.24 mg?mL-1 (r=0.999 3),RSD=1.21%.The determination results determined by HPLC-CSP were similar to those determined by optical rotation method.CONCLUSION:The established method is simple,reliable in results and reproducible,and it is applicable for the separation of the enantiomers of lactic acid and determination of the optical purity of lactic acid.

17.
China Pharmacy ; (12)2007.
Article in Chinese | WPRIM | ID: wpr-532798

ABSTRACT

OBJECTIVE:To establishment the method for the determination of content of vitamin A palmitate(VAP) and its related substances by HPLC. METHODS:The HPLC conditions were consisted of Phenomenex Luna C18 column with a mobile phase of a mixture of acetonitrile-isopropanol (90∶10) ,the detection wavelength of 328 nm,the column temperature of 30 ℃ and the flow rate of 1.0 mL?min-1. RESULTS:VAP was completely separated from impurities,the linearity range was 90~400 mg?L-1(r=0.999 2). The average recovery rate was 99.60% (RSD=1.32%). The average content of the related substances were lower than 2.53% . CONCLUSION: This accurate and reliable HPLC method is applicable for the quality control of VAP.

18.
China Journal of Chinese Materia Medica ; (24): 125-128, 2006.
Article in Chinese | WPRIM | ID: wpr-350993

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the chemical constituents of the brown alga D. divaricata, and to test cytotoxicities of the purified compounds.</p><p><b>METHOD</b>Compounds were isolated by normal phase silica gel, Sephadex LH-20 chromatography and reverse phase HPLC techniques. Their structures were elucidated by spectroscopic methods including IR, MS and NMR. Cytotoxicities were tested by MTT method.</p><p><b>RESULT</b>Eight compounds were isolated from ethanolic extract of the brown alga D. divaricata and their structures were identified as (-)-torreyol (I), 4beta, 5alpha-dihydroxycubenol (II), 3-farnesyl-p-hydroxybenzioc acid (III), chromazonarol (IV), fucosterol (V), phenyl acetylamine (VI), 4-hydroxybenzoic acid (VII) and n-hexadecanoic acid (VIII).</p><p><b>CONCLUSION</b>Compound II and IV were obtained from this alga for the first time. The others were isolated from the Dictyotaceae algae for the first time. All compounds were inactive (IC50 > 10 microg x mL(-1)) against human tumor cell lines KB, Bel-7402, PC-3M, Ketr 3 and MCF-7.</p>


Subject(s)
Humans , Cell Line, Tumor , Parabens , Chemistry , Pharmacology , Phaeophyceae , Chemistry , Stigmasterol , Chemistry , Pharmacology , Terpenes , Chemistry , Pharmacology , Xanthenes , Chemistry , Pharmacology
19.
China Pharmacy ; (12)2005.
Article in Chinese | WPRIM | ID: wpr-532675

ABSTRACT

OBJECTIVE:To establish an RP-HPLC method for content determination of s-omeprazole sodium and its related substances.METHODS:The separation of s-omeprazole sodium and the related substances was carried out on a Phenomenex Luna C18 column,the mobile phase consisted of methanol-0.033 mol?L-1 ammonium dihydrogen phosphate-triethylamine (58∶41.8∶0.2,adjusted to pH 7.0 by phosphate acid).The detection wavelength was 302 nm,the flow rate was 1.0 mL?min-1,the column temperature was 25 ℃,and the sample size was 20 ?L.RESULTS:The linear range of omeprazole sodium was 10~500 mg?L-1 (r=0.999 7).The average recovery rate was 100.27% (RSD=0.74%).The average content of the related substances in samples was 0.42%.CONCLUSION:This method is simple,accurate,specific and applicable for content determination of s-omeprazole sodium and its related substances.

20.
China Pharmacy ; (12)2005.
Article in Chinese | WPRIM | ID: wpr-530582

ABSTRACT

OBJECTIVE: To establish a HPLC method for the determination of content of entecavir and its related substances. METHODS: The HPLC condition was consisted of a Luna C18 column with a column temperature at 25 ℃, using gradient eluate method, the mobile phase was acetonitrile,with a flow rate of 1.0 mL?min-1 and detection at 254 nm. RESULTS: The calibrated linear curve of entecavir was within 0.033 45~0.167 2 mg?mL-1(r=0.999 98). The average recovery was 100.09%,RSD=0.20%, The content of related substance was 0.54%~1.48%. CONCLUSION: This accurate and reliable HPLC method is applicable for the quality control of entecavir and its related substances.

SELECTION OF CITATIONS
SEARCH DETAIL