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Article in English | WPRIM | ID: wpr-881065


Chlorogenic acid (5-CQA), neochlorogenic acid (3-CQA), and cryptochlorogenic acid (4-CQA), usually simultaneously exist in many traditional Chinese medicines (TCMs). However, insufficient attentions have been paid to the comparative metabolism study on these three isomeric constituents with similar effects on anti-inflammation until now. In this study, a novel strategy was established to perform comparative analysis of their metabolic fates in rats and elucidate the pharmacological mechanism of anti-inflammation. Firstly, diagnostic product ions (DPIs) deduced from the representative reference standards were adopted to rapidly screen and characterize the metabolites in rat plasma, urine and faeces using UHPLC-Q-TOF MS. Subsequently, Network pharmacology was utilized to elucidate their anti-inflammatory mechanism. Consequently, a total of 73 metabolites were detected and characterized, including 50, 47 and 43 metabolites for 5-CQA, 4-CQA and 3-CQA, orderly. Moreover, the network pharmacology study indicated that these three isomeric constituents and their major metabolites with similar in vivo metabolic pathways exerted anti-inflammatory effects through co-owned 20 biological processes, which involved 10 major signal pathways and 159 potential targets. Our study shed light on the similarities and differences of the metabolic profiling and anti-inflammatory activity among these three isomeric constituents and set an example for the further researches on the active mechanism of isomeric constituents existing in TCMs based on comparative metabolism study.

Article in Chinese | WPRIM | ID: wpr-879036


Astragali Radix is one of traditional Chinese medicines with effects in invigorating Qi for consolidating superficies, inducing diuresis to alleviate edema, promoting pus discharge and tissue regeneration. In recent years, the traditional Chinese medicine fermentation technology has received extensive attentions due to its high efficiency and safety. The pharmacological functions of traditional Chinese medicines could be further enhanced after microbial fermentation, which has a broad development prospects. In this paper, we summarized relevant literatures of Astragali Radix fermentation in such aspects as fermentation strains, fermentation forms, process optimization, active ingredients and pharmacological effects, in the expectation of providing a reference for development and utilization of Astragali Radix.

Astragalus Plant , Drugs, Chinese Herbal , Fermentation , Medicine, Chinese Traditional , Plant Roots
Chinese Journal of Cancer ; (12): 567-571, 2010.
Article in English | WPRIM | ID: wpr-292551


<p><b>BACKGROUND AND OBJECTIVE</b>Expression of Skp2 was related with the prognosis of several tumors. However, there was no intensive study on the relationship between Skp2 and extranodal NK/T cell lymphoma. This study was to explore the role of Skp2 in extranodal NK/T cell lymphoma.</p><p><b>METHODS</b>The clinicopathological data of 39 patients with extranodal NK/T cell lymphoma were analyzed. The expression of Skp2 was examined by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.</p><p><b>RESULTS</b>Among the patients with high expression of Skp2, complete remission (CR) rate was only 14.3% (2/14). However, CR rate among the patients with low expression of Skp2 was 68.0% (17/25). Significant difference was shown between these two groups (P < 0.001). In the group of low expression, the median overall survival (OS) was 85.59 months (95% CI: 35.83 135.34 months), the 1 and 2 year OS rates were 81% and 71%, respectively. However, in the group of high expression, the median OS was only 9.73 months (95% CI: 2.05-17.40 months), the 1 and 2 year OS rates were 42% and 14%, respectively. There was statistical difference between these two groups (P < 0.001). Multivariate analysis showed that Skp2 expression (P <0.001), LDH (P = 0.026) and ECOG PS (P = 0.003) were dependent prognostic factors of extranodal NK/T cell lymphoma.</p><p><b>CONCLUSION</b>High expression of Skp2 is an independent unfavorite adverse prognostic factor of extranodal NK/T cell lymphoma.</p>

Adolescent , Adult , Aged , Antineoplastic Combined Chemotherapy Protocols , Therapeutic Uses , Female , Follow-Up Studies , Humans , L-Lactate Dehydrogenase , Blood , Lymphoma, Extranodal NK-T-Cell , Drug Therapy , Metabolism , Pathology , Radiotherapy , Male , Middle Aged , Neoplasm Staging , Remission Induction , S-Phase Kinase-Associated Proteins , Metabolism , Survival Rate , Young Adult
Acta Pharmaceutica Sinica ; (12): 308-313, 2008.
Article in Chinese | WPRIM | ID: wpr-277856


This article describes the preparation of the N-tert-butyl-alpha-phenylnitrone (PBN) liposomes and their related characteristics. The PBN liposomes were prepared by film dispersion-supersonic method and the formula of liposomes was optimized by orthogonal uniform design. RP-HPLC was used to qualify the amount of PBN that entered into the hepatoma cells. Necrosis rate was also investigated by fluorescence activated cell sorter (FACS) after PBN liposomes transfection. Result showed that the mean particle size, entrapment efficiency, and polydispersity of the resulting PBN-liposome were 137.5 nm, 71.52% and 0.286, respectively. PBN liposomes can enter into the tumor cell stably and they have higher affinity to hepatoma cell compared with free PBN resulting in a higher necrosis rate after transfection. These results provide a potential method for early diagnosis and treatment of cancer using specific spin trapping probe targeting tumor cells.

Carcinoma, Hepatocellular , Pathology , Cyclic N-Oxides , Chemistry , Electron Spin Resonance Spectroscopy , Humans , Nanoparticles , Chemistry , Spin Labels , Spin Trapping , Methods , Tumor Cells, Cultured
Chinese Journal of Biotechnology ; (12): 131-137, 2006.
Article in Chinese | WPRIM | ID: wpr-237012


Dissimilatory Metal Reduction Bacteria play an important role in the anaerobic environment. This kind of bacteria gains energy by coupling the oxidation of organic acid or sugars to the reduction of metal oxides. The graphite electrode rode can also be used as the final electron acceptor due to its similarity to solid metal oxides. Based on this biological mechanism, Dissimilatory Metal Reduction Bacteria Rhodoferaxferrireducens was used to construct a suit of microbial fuel cells with sugars as fuel, and the process and mechanism of electricity generation was studied. Rhodoferax ferrireducens was inoculated into the anode chamber in which a graphite electrode served as the final electron acceptor and glucose as the sole electron donor. It was showed that current density was up to 158mA/m2 with the resistance of 510omega at the normal temperature (platform voltage was around 0.46V, the effectual electrode surface was 57cm2). Following 20days' growth a large amount of bacteria cells attached to the electrode surface had been observed through the SEM images. The plandtonic cell protein concentration was 140mg/L and the attached biomass of electrode surface was 1180mg/m2 determined by the Bradford method, which indicated quite a few bacteria attached to the electrode. By analyzing the voltage value measured by the data acquisition system, it was proved that microbial electricity generation attributed mainly to the electrochemically and biologically active cells attached to the electrode, while the planktonic cells had no ability to catalyze electricity generation and almost had not electrochemically and biologically active. Furthermore, this kind of microbial fuel cells exhibited a good electrochemical cycle property and proved to be efficient in biomass utilization and energy restore since other sugars like fructose, sucrose, even xylose, could be oxidized and finally decomposed. Vast waste biomass in the form of carbohydrates is discarded in the environment. Not only is contamination of the environment caused by the discarded biomass, but also abundant energy stored in the biomass is drained away in vain. The sugar-based microbial fuel cells constructed by Rhodoferax ferrireducens could effectively transform the energy stored in sugars into electricity. Meanwhile, the microbial fuel cells presented in this paper, which could work cleanly at normal temperature with a good cycle property, showed a promising future application in this field.

Bioelectric Energy Sources , Microbiology , Comamonadaceae , Metabolism , Electricity , Electron Transport , Equipment Failure , Glucose , Metabolism , Oxidation-Reduction