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1.
Article in Chinese | WPRIM | ID: wpr-906466

ABSTRACT

Objective:To establish a high performance liquid chromatography (HPLC) fingerprint of branches of <italic>Juglans mandshurica</italic> and to evaluate the quality of the samples from different producing areas and in different harvest periods. Method:Chromatographic separation was performed on an Agilent Poroshell 120 SB-C<sub>18</sub> column (2.1 mm×100 mm, 2.7 μm) for gradient elution with mobile phase of 0.2% formic acid solution (A)-0.2% formic acid acetonitrile solution (B) (0-5 min, 5%-10%B; 5-25 min, 10%-16%B; 25-40 min, 16%-22%B; 40-45 min, 22%-45%B; 45-50 min, 45%-65%B; 50-52 min, 65%-100%B; 52-55 min, 100%B) at a flow rate of 0.3 mL·min<sup>-1</sup>. The column temperature was 30 ℃ and the detection wavelength was 270 nm. The quality of branches of <italic>Juylans mandshurica</italic> was evaluated by similarity evaluation, cluster analysis, principal component analysis and partial least squares-discriminant analysis. The chemical constituents of the samples were identified by HPLC coupled with quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS/MS). The mass spectrometry was conducted in negative ion mode with electrospray ionization(ESI). Data were acquired over a range of <italic>m</italic>/<italic>z</italic> 100-1 700 for MS and <italic>m</italic>/<italic>z</italic> 50-1 700 for MS/MS. Result:A total of 19 common peaks were confirmed in 40 batches of samples, and the similarity ranged from 0.430 to 0.995, of which the similarity of samples collected in spring and winter seasons (April, May and December) was greater than 0.90, while the similarity of most samples collected in summer (July to September) was low. Multivariate statistical analysis showed that the samples were divided into two groups according to the harvest time, but there was no obvious classification rule for the samples from different producing areas. The contents of most constituents in the samples collected in spring and winter were higher than those collected in summer. The result illustrated that different harvest periods had great influence on the quality of branches of <italic>J</italic>.<italic> mandshurica</italic>. Compared with the samples collected in summer, the quality of samples collected in spring and winter was better. A total of 22 peaks were proved to be the main constituents that contributed to the difference between samples collected in different seasons. A total of 83 chemical components were identified by HPLC-Q-TOF-MS/MS, including 49 tannins, 7 organic acids, 14 naphthalene derivatives, 1 flavonoid, 6 anthracene derivatives, 2 lignans, 3 diarylheptanoids and 1 saccharide. Totally 13 common peaks were identified. Of the peaks that contributed to discriminate samples collected in different season, 19 peaks were identified and most of them were tannins. Conclusion:The established HPLC fingerprint can provide useful information for the quality evaluation of branches of <italic>J</italic>.<italic> mandshurica</italic>. Tannin is the main constituents in the samples. Harvest period has great influence on the quality of branches of <italic>J</italic>.<italic> mandshurica</italic>.

2.
Article in Chinese | WPRIM | ID: wpr-906344

ABSTRACT

Objective:To study the soil physical and chemical properties, microorganisms, and metabolites in different culture environments of <italic>Gastrodia elata</italic>, so as to provide scientific basis for subsequent cultivation of <italic>G. elata</italic> in multiple environments. Method:The tubersphere soil of <italic>G. elata</italic> cultured in different environments was collected for analyzing the soil nutrients, microbial numbers, and metabolite differences using the agrochemical method, plate-count method, and gas chromatography-time-of-flight mass spectrometry (GC-TOF-MS)-based non-targeted metabonomic approach. Result:The analysis of soil physical and chemical properties revealed the highest soil moisture, pH, available potassium, and available phosphorus in the spinney and the highest electrical conductivity, total nitrogen, alkali-hydrolyzable nitrogen, and organic matter in the pinewood. As demonstrated by the quantitative analysis of soil microorganisms, the cultivable microorganisms were bacteria, actinomycetes, and fungi, with the bacterial population and total microbial biomass in the spinney and the number of fungi and actinomycetes in the barren slope detected to be the largest. The ratio of bacteria to fungi (B/F value) in the pinewood was the highest, while that in the barren slope was the lowest. The results of metabonomic research demonstrated that the compositions and quantities of soil metabolites in the spinney (Z group), pinewood (S group), and barren slope (HD group) varied. Through comparisons between S and Z groups, between HD and Z groups, as well as between HD and S groups by orthogonal partial least squares discriminant analysis (OPLS-DA), 18, 35, and 24 differential metabolites were separately screened out, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis yielded 5, 9, and 13 metabolic pathways. There existed a significant causal relationship of the soil physical and chemical properties and microbial numbers with the metabolites. Conclusion:The soil physical and chemical properties, microbial numbers, and metabolite changes differed significantly in different culture environments of <italic>G. elata</italic>, which were sorted by the suitability in a descending order as follows: spinney > pinewood >barren slope. The soil physical and chemical properties and microbial numbers are the crucial factors driving changes in soil metabolites, suggesting that regulating the soil physical and chemical characteristics and microbial characteristics in the culture environment is an important mechanism for maintaining the <italic>G. elata</italic>-soil-microbial symbiotic system.

3.
Article in Chinese | WPRIM | ID: wpr-921817

ABSTRACT

Tongsaimai Tablets/Capsules are composed of Lonicerae Japonicae Flos, Angelicae Sinensis Radix, Achyranthis Bidentatae Radix, Codonopsis Radix, Dendrobii Caulis, Astragali Radix, Scrophulariae Radix, and Glycyrrhizae Radix et Rhizoma, and are effective in promoting blood circulation, removing blood stasis, supplementing Qi, and nourishing Yin. It is widely used in the treatment of peripheral vascular diseases. With 40 years of clinical application, it has accumulated substantial research data and application experience. Its good clinical efficacy and pharmacoeconomic benefits in improving the clinical symptoms of peripheral vascular diseases have been confirmed by relevant research. Meanwhile, this drug has also been recommended by many expert consensus, guidelines, and teaching materials, serving as one of the most commonly used Chinese patent medicines in clinical practice. To further improve the understanding of the drug among clinicians and properly guide its clinical medication, the China Association of Chinese Medicine took the lead and organized experts to jointly formulate this expert consensus. Based on the questionnaire survey of clinicians and the systematic review of research literature on Tongsaimai Tablets/Capsules with clinical problems in the PICO framework, the consensus, combined with expert experience, concludes recommendations or consensus suggestions by GRADE system with the optimal evidence available through the nominal group technique. This consensus defines the indications, usage, dosage, course of treatment, medication time, combined medication, and precautions of Tongsaimai Tablets/Capsules in the treatment of peripheral vascular diseases, and explains the safety of its clinical application. It is recommended for clinicians and pharmacists in the peripheral vascular department(vascular surgery), traditional Chinese medicine surgery(general surgery), and endocrinology department of hospitals at all levels in China.


Subject(s)
Capsules , Consensus , Drugs, Chinese Herbal , Humans , Medicine, Chinese Traditional , Peripheral Vascular Diseases , Tablets
4.
Article in English | WPRIM | ID: wpr-887747

ABSTRACT

Malocclusion is one of the three most common oral diseases reported by World Health Organization(WHO). In China, its incidence rate is rising. Malocclusion seriously affects the dental and maxillofacial function, facial appearance and growth development of nearly 260 million children in China, and what is more, it affects their physical and mental health development. Malocclusion occurrence is related to genetic and environmental factors. Early treatment of malocclusion can create a good dental and maxillofacial development environment, correct abnormal growth and control the adverse effects of abnormal genetic factors. It can effectively reduce the prevalence of children's malocclusion and enhance their physical and mental health. This is an urgent need from the economic perspective of our society, so it has great practical and social significance. Experts from the project group "standard diagnose and treatment protocols for early orthodontic intervention of malocclusions of children" which initiated by China National Health Institute of Hospital Administration wrote the "China Experts' Consensus on Preventive and Interceptive Orthodontic Treatments of Malocclusions of Children", which aims to guide and popularize the clinical practice, improve the clinical theory and practice level, and accelerate the disciplinary development of early treatment of children's malocclusion in China. The consensus elaborates the harmfulness of malocclusion and the necessity of early treatment, and brings up the principles and fundamental contents. Based on the law of dental and maxillofacial development, this paper puts forward the guiding suggestions of preventive and interceptive treatments in different stages of dental development ranging from fetus to early permanent dentition. It is a systematic project to promote and standardize the early treatment of malocclusion. Through scientific and comprehensive stratified clinical practice and professional training, the clinical system of early treatment of malocclusion in China will eventually be perfected, so as to comprehensively care for children's dental and maxillofacial health, and improve their oral and physical health in China.


Subject(s)
Child , China/epidemiology , Consensus , Dental Care , Humans , Malocclusion/prevention & control , Orthodontics, Interceptive
5.
Article in Chinese | WPRIM | ID: wpr-880058

ABSTRACT

OBJECTIVE@#To explore the value of PCR-flow fluorenscence immunmicrobeads assay in prenatal gene diagnosis of thalassemia.@*METHODS@#A total of 1001 pregnant women and their couples checked in the First Affiliated Hospital of Sun Yat-Sen University from January 2016 to August 2019 were selected. Both pregnant women and their spouses were the carriers of thalassemia gene. Samples such as amniotic fluid, were used to extract genomic DNA at the right time. Parallel detection of α- and β- thalassemia genes to samples should be carried out by PCR-flow cytometric fluorescence hybridization and traditional multiple Gap-PCR and PCR-RDB techniques. The consistency of two methods in gene diagnosis of thalassemia was evaluated by analyzing the results of detection.@*RESULTS@#389 normal genotypes (38.86%, 389/1001) and 59 abnormal genotypes (61.14%, 612/1001) was cheked out by the two methods, including 416 cases of α-thalassemia, 162 cases of β-thalassemia and 34 cases of αβ- complex thalassemia. The main genotypes of α-thalassemia were --@*CONCLUSION@#Guangzhou is a area with high incidence of thalassemia, and the genetic types of thalassemia are complex and diverse. Prenatal diagnosis is the final barrier to the prevention of thalassemia. PCR flow-cytometric fluorescence hybridization, as a simple and fast technique, combined with traditional techniques in parallel contributed to the accuracy of prenatal gene diagnosis of thalassemia.


Subject(s)
China , Female , Genotype , Humans , Mutation , Polymerase Chain Reaction , Pregnancy , Prenatal Diagnosis , alpha-Thalassemia/genetics , beta-Thalassemia/genetics
6.
Article | IMSEAR | ID: sea-210360

ABSTRACT

Background: A novel method for inducing hyphae formation, was described.Methods:Mycelia of experimental and clinical strains from 100 patients with oral lichen planuswere cultured in RPMI 1640 medium with 56°C or 121°C inactivated calf sera. After these strains were cultured for 2 h to 7 days, the average hyphae formation rate was observed under light microscopy. Results: The 121°C inactivated calf serum showed better outcomes than 56°C inactivated calf serum in terms of time and amounts of hyphae products. Conclusions:For Candida albicanshyphae culture, the 121°C inactivated serum is superior to the 56°C inactivated serum

7.
Article in Chinese | WPRIM | ID: wpr-788963

ABSTRACT

Corona Virus Disease 2019 (COVID-19) has broke out in 29 countries and regions in the world in a short period since December 2019. Various measures of prevention and control have been taken all over China. At present, the epidemic situation shows a positive trend, however, there are still a few new comfirmed cases in some area of China. So the job and thinking of epidemic prevention still cannot be relaxed. As an important specialty of stomatology, the risk of cross infection is high in oral medicine. Establishing an effective system of pre- inspection and triage and adopting strict measures of prevention and control are essential. According to the prevention and control situation of COVID- 19, the characteristics of hospital infection, pre examination, protective measures, the home management strategies of oral mucosal diseases during the epidemic period and the differential diagnosis between COVID-19 and common oral muco- sal diseases with fever were summarized and recommended in this paper.

8.
Article in Chinese | WPRIM | ID: wpr-865338

ABSTRACT

Objective:To investigate the in vitro interaction between antifungals and tacrolimus against Fusarium solani. Methods:According to Clinical and Laboratory Standards Institute(CLSI) M27-Ed4 and M38-A3, 24 strains of Fusarium solani isolated from clinic were used to test the minimal inhibitory concentration (MIC) of the four antifungals.Chessboard microdilution method was used to evaluate the interaction of tacrolimus (FK506) combined with four antifungals in vitro.The study protocol was approved by the Ethics Committee of Hainan Eye Hospital (No.2017-006). Results:The MIC ranges of fluconazole, voriconazole, natamycin and amphotericin B against 24 strains of Fusarium were 64-256 μg/ml, 1-8 μg/ml, 2-8 μg/ml and 1-8 μg/ml, respectively.Tacrolimus combined with fluconazole, voriconazole, natamycin and amphotericin B had synergistic effects on 12.5% (3/24), 4.2% (1/24), 16.7% (4/24) and 29.2% (7/24) Fusarium, respectively.There were no antagonistic effect in this study. Conclusions:Fusarium is sensitive to natamycin in vitro and partly sensitive to amphotericin B and voriconazole.Synergistic effects exist in few strains of Fusarium but no antagonistic effect.

9.
Chinese Journal of Stomatology ; (12): 123-128, 2020.
Article in Chinese | WPRIM | ID: wpr-799361

ABSTRACT

Burning mouth syndrome (BMS) is a complex chronic disorder characterized symptomatically by burning sensation mainly localized in the tongue with no specific lesions. Its etiology is not fully understood. Due to the persistent pain and unsatisfactory outcome of treatment, patients with high expectation when seeking medical advice easily feel discontent after treatment, which might cause medical dispute. This review introduces the epidemiology, manifestations, etiology, diagnosis, classification and treatment of BMS. The authors hope the current advances about BMS, mainly focus on the etiology of biological nerve and social psychology, may help the clinicians in treatment of BMS.

10.
Article in Chinese | WPRIM | ID: wpr-828015

ABSTRACT

Ginkgo biloba and Panax notoginseng are both herb medicines for cerebrovascular disease, and play an active role in treating ischemic cerebrovascular disease(ICVD). Their mechanisms of action include antioxidant stress, nerve protection, vascular protection. According to the comparative study of literatures, G. biloba has a certain protective effect from the early stage of free radical formation throughout the whole process of causing cell inflammation and apoptosis in antioxidant stress; while P. notoginseng has mainly anti-inflammatory, anti-apoptosis effects. In the nerve protection and repair of nerve damage caused by glutamate, both could promote neurogenesis, repair damaged axons and protect nerve cells. In addition, G. biloba could also relieve neurotoxicity caused by glutamate damage, while P. notoginseng have a unique effect in repairing blood-brain barrier(BBB) and blood vessel regeneration. In clinic, they are used as auxiliary drugs in combination with thrombolytic therapy, and play curative effects in alleviating inflammation, eliminating edema, improving the cure rate and the prognosis. For cerebral diseases caused by chronic cerebral hypoperfusion, G. biloba could reduce inflammation and improve cognition. In addition, G. biloba could protect neurocyte by adjusting the secretion of dopamine in vivo, and has a certain effect on antidepressant diseases, which however needs further studies.


Subject(s)
Brain Ischemia , Drug Therapy , Ginkgo biloba , Humans , Panax notoginseng , Phytotherapy , Plant Extracts , Therapeutic Uses , Plants, Medicinal
11.
Article | IMSEAR | ID: sea-210066

ABSTRACT

Mandibular osteomyelitis in a patient with psoriasis is an uncommonly clinical manifestation while there is an increasing number of reports and studies on involvements of stomatology in psoriasis, especially the death of a patient via or not via Allogeneicbone marrow transplantation has never been reported.To review the management and possible mechanisms in pathogenesis and treatment of psoriasis, as well as the relative involvements between stomatology and psoriasis the typical case with pictures and files is reviewed and literature is collected.Wekeepthe knowledge that psoriasis is either a primary keratinocyte disorder or an immunocyte-mediated chronic skin inflammatorydisease while bone marrow is under suspected for immunopathogenesis. More association of stomatologic conditions with psoriasis is emerging. Conclusively, allogeneicBMT and new knowledge are worth to be stressed by both stomatological and dermatological doctors. Further insights of this kind of auto immunologic disease are under its developing.

12.
Article in Chinese | WPRIM | ID: wpr-771515

ABSTRACT

Ralstonia solanacearum strain PRS-84 used in this study was isolated from diseased Pogostemon cablin plants in our previous study.The competent cells of R.solanacearum strain PRS-84 were transformed by electroporation with Tn5 transposon and then were plated on TTC agar plates containing kanamycin to select for kanamycin-resistant colonies.The detection of kanamycin-resistant gene in kanamycin-resistant colonies was performed by PCR.Further,the flanking fragments of Tn5 transposon insertion site in the mutants were amplified by inverse PCR,and the flanking fragments were sequenced and analyzed.The results indicated that the kanamycin-resistant colonies were obtained in the transformation experiment of R.solanacearum strain PRS-84 by electroporation with Tn5 transposon.A specific band of approximately 700 bp was amplified by PCR from kanamycin-resistant colonies.The flanking sequences of Tn5 transposon insertion site in the transformants were obtained by inverse PCR.After sequencing and sequence analysis of Tn5 transposon insertion site in mutants,we preliminarily speculated that the Tn5 transposon inserted in the typ A gene,rec O gene and gid A gene in three mutants,respectively.A random mutagenesis system of R.solanacearum strain PRS-84 by electroporation with Tn5 transposon has been established,and the Tn5 insertion mutants have been obtained.This study might facilitate the creation of mutant library and the discovery of the virulence gene of R.solanacearum isolated from P.cablin.


Subject(s)
DNA Transposable Elements , Electroporation , Genes, Bacterial , Mutagenesis, Insertional , Pogostemon , Microbiology , Ralstonia solanacearum , Genetics , Virulence
13.
Article in Chinese | WPRIM | ID: wpr-751758

ABSTRACT

The combined technique of Liquid chromatography-mass spectrometry (LC/MS) is regarded as one of the most powerful analytical techniques for the analysis of traditional Chinese medicine (TCM) since it combines the high efficiency of LC separation with the confident structural identification of mass spectrometric detection.Studieson pharmacodynamic material basisof TCM are key to TCM modern research.This paper briefly reviewed its application in the study of chemical components,serum pharmacochemistry and metabonomics of TCM,as well as the study of the fingerprints of herbal drugs,cell membrane chromatography and affinity ultrafiltration mass pectrometry,which offered reference for further application of LC/MS in traditional Chinese medicine.

14.
Article in Chinese | WPRIM | ID: wpr-905528

ABSTRACT

Isokinetic muscle strength test has been applied to athletes, healthy people and patients with muscular dystrophy, and some results have been achieved. However, due to the different parts, purposes, intensity and methods of training, the information integration are unclear, lacking a complete overview of clinical application of isokinetic muscle test and training. This paper mainly reviewed the current research status of isokinetic muscle strength test and training in various fields of trunk muscle group, including low back pain, stroke, and sports medicine, etc. In addition, the indexes and influencing factors of trunk muscle group and core muscle group were briefly constructed, and some common research methods were integrated.

15.
Chinese Journal of Endemiology ; (12): 932-935, 2019.
Article in Chinese | WPRIM | ID: wpr-800955

ABSTRACT

Objective@#To investigate the epidemiology and related clinical features of hemorrhagic fever with renal syndrome (HFRS) in Qingdao, and provide relevant evidence for clinical diagnosis and treatment.@*Methods@#The retrospective analysis method was used to collect the epidemiological and clinical data of 104 patients with HFRS diagnosed in the Affiliated Hospital of Qingdao University from January 2014 to December 2018, and the general condition, clinical symptoms, laboratory examination and exposure history of the patients were analyzed.@*Results@#One hundred and four patients with HFRS were aged from 10 to 87 years old, including 78 males and 26 females; mainly living in rural areas, accounting for 90.38% (94/104); and 54 cases from the Department of Nephrology, accounting for 51.92%. October to January of the following year was the main onset time, accounting for 68.27% (71/104). The clinical manifestations were 98 cases of fever, accounting for 94.23%; 66 cases of nausea and vomiting, accounting for 63.46%; and 57 cases of dizziness and headache, accounting for 54.81%. Laboratory examination showed more thrombocytopenia, urinary protein and occult blood positive, D-dimer elevation, C-reactive protein and procalcitonin elevation. Only 8 cases had a history of direct, indirect or suspicious contact.@*Conclusions@#The clinical features of HFRS are atypical. The main symptoms are fever, nausea and vomiting, dizziness and headache. Men are higher than women. The place of residence is mainly in rural areas. The exposure history is mostly unclear. Diagnosis should be combined with laboratory findings and early treatment to improve clinical outcomes.

16.
Chinese Journal of Stomatology ; (12): 808-814, 2019.
Article in Chinese | WPRIM | ID: wpr-800035

ABSTRACT

Temporomandibular joint (TMJ) related signs and symptoms are frequently found in adolescent and adult orthodontic patients. TMJ health has long been considered important in orthodontic treatment for many decades, and routine screening and management of TMJ problems should be emphasized in orthodontic population. This paper was to review the TMJ problems in orthodontic patients and to set the strategy in clinical orthodontics.

17.
Article in Chinese | WPRIM | ID: wpr-843402

ABSTRACT

Objective: To study the effect of the number of thrombectomy on the prognosis of patients with acute ischemic stroke treated with mechanical thrombectomy (MT). Methods: Retrospective analysis was performed in 88 acute ischemic stroke patients treated with MT in Shanghai Ninth People's Hospital from March 2013 to April 2018. Based on the number of thrombectomy, all the patients were divided into the group with lower number of thrombectomy (group A) ( ≤ 2 times of thrombectomy) and the group with higher number of thrombectomy (group B) (>2 times of thrombectomy). Revascularization rate, incidence of intracerebral hemorrhage, score of National Institute of Health Stroke Scale (NIHSS) and mortality at 7 d after surgery, modified Rankin score (mRs) and mortality at 90 d after surgery were compared between the two groups. Results: Compared with group B, there were no statistically significant differences in the incidence of intracerebral hemorrhage, and rates of symptom improvement at 7 d and good prognosis at 90 d in group A (all P>0.05). But the mortality at 7 d and 90 d in group A was lower than that in group B (P=0.003, P=0.031), and the rate of successful revascularization in group A was significantly higher than that in group B (P=0.010). Conclusion: Multiple thrombectomy is not significantly correlated with intracerebral hemorrhage, early symptom improvement and good prognosis at 90 d, but it is correlated with the rates of revascularization and mortality.

18.
Chinese Pharmacological Bulletin ; (12): 321-326, 2019.
Article in Chinese | WPRIM | ID: wpr-857333

ABSTRACT

Aim: To investigate the protective effect of trans-anethole on the PC12 cell injury induced by sodium azide. Methods: PC12 cells were exposed to sodium azide with concentration ranged from 9 ∼ 54 mmol · L-1. The survival rate of the cells was determined by CCK-8 assay. The cell morphology was detected with the inverted microscope; the apoptosis of cells was detected by Hoechst 33258 staining; the content of LDH, MDA levels and antioxidant enzyme activities of SOD were determined using the assay kits according to the manufacturer's protocol. Results PC12 cell injury induced by sodium azide presented a dose-effect relationship. The PC12 cell viability was not changed when treated by irans-anethole for 48 h. Pretreatment of PC12 cells with 10 μmol · L-1 and 60 μmol · L∼ trans-anethole for 8 h could obviously reduce cell injury, reduce LDH leakage and MDA level, as well as increase the antioxidant enzyme activities of SOD induced by sodium azide. Conclusions: Trans-anethole exerts significant protective effect on PC12 cell injury induced by sodium azide. The protective mechanism might be executed by increasing the activities of antioxidant enzymes.

19.
Chinese Journal of Endemiology ; (12): 932-935, 2019.
Article in Chinese | WPRIM | ID: wpr-824080

ABSTRACT

Objective To investigate the epidemiology and related clinical features of hemorrhagic fever with renal syndrome (HFRS) in Qingdao,and provide relevant evidence for clinical diagnosis and treatment.Methods The retrospective analysis method was used to collect the epidemiological and clinical data of 104 patients with HFRS diagnosed in the Affiliated Hospital of Qingdao University from January 2014 to December 2018,and the general condition,clinical symptoms,laboratory examination and exposure history of the patients were analyzed.Results One hundred and four patients with HFRS were aged from 10 to 87 years old,including 78 males and 26 females;mainly living in rural areas,accounting for 90.38% (94/104);and 54 cases from the Department of Nephrology,accounting for 51.92%.October to January of the following year was the main onset time,accounting for 68.27% (71/104).The clinical manifestations were 98 cases of fever,accounting for 94.23%;66 cases of nausea and vomiting,accounting for 63.46%;and 57 cases of dizziness and headache,accounting for 54.81%.Laboratory examination showed more thrombocytopenia,urinary protein and occult blood positive,D-dimer elevation,C-reactive protein and procalcitonin elevation.Only 8 cases had a history of direct,indirect or suspicious contact.Conclusions The clinical features of HFRS are atypical.The main symptoms are fever,nausea and vomiting,dizziness and headache.Men are higher than women.The place of residence is mainly in rural areas.The exposure history is mostly unclear.Diagnosis should be combined with laboratory findings and early treatment to improve clinical outcomes.

20.
Practical Oncology Journal ; (6): 352-356, 2019.
Article in Chinese | WPRIM | ID: wpr-752867

ABSTRACT

Objective The aim of this study was to evaluate the clinical efficacy and hematologic toxicity of docetaxel in the treatment of metastatic breast cancer patients in different age groups. Methods The clinical data of three groups of single-agent do-cetaxel in the treatment of metastatic breast cancer patients were retrospectively analyzed. The clinical efficacy of docetaxel was ana-lyzed statistically. Results The objective response rate(ORR) and clinical beneficial rate( CBR) in the group of age < =45 year group were 30. 6% and 38. 7% ,and the ORR and CBR in the 46~59 age were 27. 5% and 35. 7% ,while ORR and CBR in the≥60 age group were 20. 0% and 27. 5% . The median progress free survival( PFS) in three groups was 6. 0(2. 9 ~9. 1) months,5. 0 (2. 9~7. 1)months and 4. 0(3. 3~4. 7) months,respectively. There was no statistical difference in three different age groups(P=0. 477,0. 492 and 0. 460,respectively). Cox regression analysis showed that the docetaxel rescue treatment of PFS had not associated with clinical stage,pathological type,ipsilateral axillary lymph node metastasis,disease free survival,unit dose of body surface area dose,number of rescue treatment lines,number of recurrence and metastasis,etc. in three different age groups of patients with meta-static breast cancer. Hematological toxicity was mainly manifested by a decrease in white blood cells and neutrophils,and docetaxel has less effect on hemoglobin and platelets. Conclusion The clinical efficacy and hematologic toxicity of docetaxel have a certain de-gree of correlation with ages. With the increase of age,the clinical curative effect is reduced and the blood toxicity is aggravated.

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