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1.
Indian Pediatr ; 2020 Feb; 57(2): 138-142
Article | IMSEAR | ID: sea-199478

ABSTRACT

Objective: To investigate the prevalence and risk factors of congenital heart disease inYunnan, China which has diverse ethnic groups. Methods: This cross-sectional studyenrolled 244,023 children from 2010 to 2015. To diagnose CHD, a conventional physicalexamination was used to screen suspicious cases, which were further confirmed byechocardiography. Results: A total of 1695 children were diagnosed with CHD. Theestimated prevalence was 6.94%. Atrial septal defects were the most common cardiacabnormalities. A higher prevalence of CHD was observed with preterm birth, low birthweight, maternal age ≥35 years, and high-altitude regions. The prevalence also showeddifferences between diverse ethnic groups. Conclusion: The prevalence of CHD in Chinamay have ethnic differences.

2.
Article in Chinese | WPRIM | ID: wpr-781701

ABSTRACT

OBJECTIVE@#To study the value of body fat mass measured by bioelectrical impedance analysis (BIA) in predicting abnormal blood pressure and abnormal glucose metabolism in children.@*METHODS@#Stratified cluster sampling was used to select the students aged 6-16 years, and a questionnaire survey and physical examination were performed. The BIA apparatus was used to measure body fat mass. Body mass index (BMI), body fat mass index (FMI), and fat mass percentage (FMP) were calculated. Fasting blood glucose level were measured.@*RESULTS@#A total of 14 293 children were enrolled, among whom boys accounted for 49.89%. In boys and girls, the percentile values (P, P, P, P, P, P, P, P) of FMI and FMP fitted by the LMS method were taken as the cut-off values. Based on the receiver operating characteristic curve analysis, the P values with a better value in predicting abnormal blood pressure and blood glucose metabolism were selected as the cut-off values for excessive body fat. When FMI or FMP was controlled below P, the incidence of abnormal blood pressure or abnormal glucose metabolism may be decreased in 8.25%-43.24% of the children.@*CONCLUSIONS@#The evaluation of obesity based on FMI and FMP has a certain value in screening for hypertension and hyperglycemia in children, which can be further verified in the future prevention and treatment of obesity and related chronic diseases in children.


Subject(s)
Adipose Tissue , Adolescent , Blood Pressure , Body Composition , Body Mass Index , Child , Electric Impedance , Female , Glucose , Humans , Male
3.
Article in Chinese | WPRIM | ID: wpr-787707

ABSTRACT

To compare the power of dyslipidemia diagnosis by different sets of cut points in the prediction of cardiovascular metabolic risk factors and identify the appropriate cut points for the diagnosis of dyslipidemia in children and adolescents in China. Data were obtained from the baseline survey of 'School-based Cardiovascular and Bone Health Promotion Program' in Beijing in 2017. Dyslipidemia was diagnosed by using two set of cut points. Receiver operating characteristic curve analysis was conducted to assess the power of dyslipidemia diagnosis by the two set of cut points to predict the prevalence of hypertension, obesity, high fat mass percentage and impaired fasting glucose. A total of 14 390 children and adolescents were in included in the study. The prevalence rates of high TC, high LDL-C, low HDL-C, and high TG in the participants were 2.7, 2.7, 14.4, and 3.7 according to 'Chinese Reference Standard', and 5.0, 3.7, 13.3, and 3.5 according to 'China Expert Consensus'. Low HDL-C and high TG defined by the 'Chinese Reference Standard' had better performance for the prediction of high fat mass percentage and obesity in boys, but worse performance in girls (<0.001). Using 'China Reference Standard' can increase the true positive rate in the prediction of obesity or high fat mass percentage in boys, and reduce the false positive rate in girls. The cut points for the diagnosis of dyslipidemia in Chinese children and adolescents need to be further validated by using national representative sample and in longitudinal study.

4.
Article in English | WPRIM | ID: wpr-826614

ABSTRACT

The aim of this study was to identify some biomarkers for predicting lymph node metastasis and prognosis of human epidermal growth factor receptor 2 (Her-2)-positive breast cancer (BC). We analyzed correlations between microRNAs (miRNAs) and the prognosis of patients with BC based on data collected from The Cancer Genome Atlas (TCGA) database. The expression levels of miR-455, miR-143, and miR-99a were measured in clinical samples of Her-2-positive BC patients with different degrees of lymph node metastasis. We investigated the impacts of overexpressed miR-455 on the proliferation and invasiveness of MDA-MB-453 cells and measured its effects on the expression of long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) by quantitative real-time polymerase chain reaction (qRT-PCR). The expression of miR-455 was significantly and positively correlated to the prognosis and overall survival (OS) of the BC (P=0.028), according to TCGA information. The expression level of miR-455 was positively correlated with OS and relapse-free survival (RFS) of patients with Her-2-positive BC, and was negatively correlated with the number of metastatic lymph nodes (P<0.05). Transwell assay suggested that MDA-MB-453 cells became much less invasive (P<0.01) after being transfected with miR-455 mimics. During the qRT-PCR, the expression level of MALAT1 declined significantly after transfection (P<0.01). Overexpressed miR-455 significantly inhibited the proliferation and migration of MDA-MB-453 cells and the expression of MALAT1. We conclude that miR-455 may be a useful potential biomarker for forecasting lymph node metastasis and the prognosis of Her-2-positive BC patients. miR-455 may play an important role in lymph node metastasis of BC by interacting with MALAT1.

5.
Article in Chinese | WPRIM | ID: wpr-905599

ABSTRACT

Objective:To propose a motor imagery paradigm with regular rhythm to improve the accuracy of classification in brain-computer interface based on motor imagery. Methods:Ten untrained healthy subjects were asked to image the movement guided by the picture accompanied with regular rhythm. The common spatial pattern feature extraction algorithm and Fisher classifier were used for classification, and compared with the traditional paradigm and the motor imagery paradigm accompanying with irregular rhythm. Results:More desynchronization was found as motor imagery with regular rhythm, while the classification accuracy improved. Conclusion:Motor imagery with regular rhythm gives a new idea for active rehabilitation training based on brain-computer interface.

6.
Article in Chinese | WPRIM | ID: wpr-905576

ABSTRACT

Objective:To propose a motor imagery paradigm with regular rhythm to improve the accuracy of classification in brain-computer interface based on motor imagery. Methods:Ten untrained healthy subjects were asked to image the movement guided by the picture accompanied with regular rhythm. The common spatial pattern feature extraction algorithm and Fisher classifier were used for classification, and compared with the traditional paradigm and the motor imagery paradigm accompanying with irregular rhythm. Results:More desynchronization was found as motor imagery with regular rhythm, while the classification accuracy improved. Conclusion:Motor imagery with regular rhythm gives a new idea for active rehabilitation training based on brain-computer interface.

7.
Chinese Traditional Patent Medicine ; (12): 1233-1237, 2018.
Article in Chinese | WPRIM | ID: wpr-710285

ABSTRACT

AIM To study the influence and action mechanism of effective components in compatible solution of Sparganii Rhizoma-Curcumae Rhizoma on pelvic adhesions of model rats with chronic pelvic inflammatory disease.METHODS Laboratory rats were randomly assigned into six groups,a sham-operated group,a model group,a positive Fukeqianjin tablets group,compatible solution groups (40 g/kg high-dose group,20 g/kg medi um-dose group,and 10 g/kg low-dose group).Except rats of the sham-operated group,those in the other groups were induced into models of chronic pelvic inflammatory disease by mechanical bacterial strain implantation,after which a 20 d corresponding drug administration by oral gavage for each group was initiated.After completion of the last drug administration,Verco criterion was taken as the reference to evaluate pelvic adhesions,HE staining was performed on uterine tissues for pathological scoring,ELASA method was applied to measuring serum IL-1β and TNF-α levels,and Western blot method was used to detect the expression levels of proteins FGF-2 and IGF-1 in uterine tissues.RESULTS Compared with the model group,the high-dose compatible solution group had a much sharper fall in Verco scores (P < 0.05),and remarkably decreased serum IL-1β and TNF-α levels (P < 0.01);both high-dose and medium-dose groups manifested with notably alleviated degenerative necrosis and lowered scores of chronic inflammation infiltration and epithelial hyperproliferation (P < 0.01 or P < 0.05),and a distinct reduction in the expression of proteins FGF-2 and IGF-1 (P < 0.05,P < 0.01).CONCLUSION The significant improvement of the chronic pelvic inflammatory disease in model rats due to the effective components in the compatibile solution of Sparganii Rhizoma-Curcumae Rhizoma may be associated with inhibited release of inflammatory mediators IL-1β and TNF-α,and the reduced expression of proteins FGF-2 and IGF-1 as well.

8.
Article in Chinese | WPRIM | ID: wpr-838146

ABSTRACT

Objective To explore the effect of informationized time tracking management mode on the speed of treatment of acute ischemic stroke (AIS). Methods From Aug. 2016 to Jun. 2018, a total of 475 AIS patients receiving intravenous thrombolysis and/or intravascular treatment in Stroke Center of Changhai Hospital of Navy Medical University (Second Military Medical University) were divided into time tracking group (n=291) and non-time tracking group (n=184). The time indicators were compared between the two groups, including onset-to-door time (ODT), door-to-imaging time (DIT), imaging-to-needle time (INT), onset-to-needle time (ONT), imaging-to-puncture time (IPT), onset-to-puncture time (OPT), door-to-needle time (DNT) and door-to-puncture time (DPT). The correlations between ODT and DNT, and ODT and DPT were analyzed using Spearman correlation analysis in the non-time tracking group and the time tracking group. Results The DNT of the patients with intravenous thrombolysis was significantly shorter in the time tracking group than that in the non-time tracking group (27 [23, 36] min vs 33 [23, 43] min, Z=2.123, P<0.05). There was a significant negative correlation between ODT and DNT of the patients with intravenous thrombolysis in the non-time tracking group (r=–0.293, P=0.001), while there was no correlation in the time tracking group (r=–0.034, P=0.723). The ODT and OPT of the patients with endovascular treatment were significantly longer, and DIT was significantly shorter in the time tracking group than those in the non-time tracking group (246 [113, 431] min vs 166 [82, 299] min, 342 [211, 519] min vs 277 [142, 406] min and 18 [14, 23] min vs 26 [16, 34] min; Z=2.667, 2.182 and 4.077, all P<0.05). There was a significant negative correlation between ODT and DPT of the patients with endovascular treatment in the non-time tracking group (r=–0.234, P=0.024), while there was no correlation between ODT and DPT in the time tracking group. (r=–0.056, P=0.424). Conclusion The informationized time tracking management mode can improve the treatment efficiency of acute ischemic stroke and eliminate the influence of ODT on DNT and DPT.

9.
Article in Chinese | WPRIM | ID: wpr-838145

ABSTRACT

Objective To explore the value of intelligent early warning system in pre- and in-hospital connection in the treatment of stroke. Methods The clinical data of the patients with suspected stroke, who received treatment and prehospital warning with intelligent early warning system in Stroke Center of Changhai Hospital of Navy Medical University (Second Military Medical University) between Nov. 2017 and Jun. 2018, were retrospectively analyzed. Pre-hospital warning was defined as 120 alerting stroke teams using software before transferring patients to the target hospital. According to the in-hospital treatment methods, the patients were divided into non-stroke group, conservative treatment group, thrombolysis group, thrombectomy group, bridging group and cerebral hemorrhage group. The efficiency in each link of the intelligent early warning system, the ability of emergency doctor diagnosing stroke, the ability of emergency doctor diagnosing severe stroke using gaze-face arm speech time (G-FAST) scale, the speed of first aid response of the attending physician and the door-in-to-door-out (DIDO) time in primary stroke center were evaluated in each group. Results A total of 248 patients were included in this study, including 24 non-stroke patients and 224 stroke patients (101 patients in the conservative treatment group, 23 patients in the thrombolysis group, 32 patients in the thrombectomy group, 22 patients in the bridging group and 46 patients in the cerebral hemorrhage group). The median primary screening time, early warning time and response time of 248 patients were 28.0 (13.0, 92.5), 11.0 (7.3, 15.3) and 19.0 (13.0, 35.0) s, respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of 6 pre-hospital emergency physicians diagnosing severe stroke using G-FAST scale were 84%, 71%, 83%, 72% and 79%, respectively. Eight patients with severe stroke underwent whole-process monitoring of pre-hospital treatment, and had a median transport time of 113 (82, 142) min and a median DIDO time of 84 (12, 125) min. Conclusion Intelligent early warning system can realize the automation of data acquisition for transfer time points, making the data more comprehensive and reliable. It is helpful to analyze the efficiency of emergency physicians, drivers, stroke teams and primary stroke center in pre- and in-hospital connection in the treatment of stroke, so as to shorten the pre-hospital treatment time and improve the pre-hospital treatment efficiency.

10.
Journal of Preventive Medicine ; (12): 46-49,54, 2018.
Article in Chinese | WPRIM | ID: wpr-792695

ABSTRACT

Objective To evaluate the association between aortic arterial stiffness and target organ damage, including renal function decline and left ventricular diastolic dysfunction among elderly diabetic patients and to provide theoretical basis for early prevention and intervention. Methods Brachial-ankle-artery pulse wave velocity(baPWV)reflecting aortic arterial stiffness was observed in 151 diabetic patients with(n=78)or without hypertension(n=73). Clinical data were collected, cardiac structure and function were assessed by echocardiography. Estimated glomerular filtration rate(eGFR)was calculated by CKD-EPI fomula. Results Of 151 subjects, the average age was(75.05±10.13)years and the average baPWV values was (1 803.85±491.04)mm/s. Patients with both diabetes and hypertension had significantly higher baPWV values[(2006.49± 536.70)mm/s]than that of diabetic patients[(1596.96 ±331.02)mm/s, P<0.01]. Multiple linear regression analysis demonstrated that baPWV was negatively associated with E/A ratio and eGFR(β'=-0.249, -0.225, P<0.05), but positively associated with hypertension(β'=2.008, P<0.05). By applying area under the receiver operator characteristic curve, baPWV had powerful predictive power for renal impairment(AUC=0.695, 95% CI: 0.548-0.842)and left ventricular diastolic dysfunction(AUC=0.687, 95%CI: 0.532-0.842). Conclusion Patients with both diabetes and hypertension had significantly higher baPWV values than that of diabetic patients. Higher baPWV was independently associated with greater burden of sub-clinical disease in renal impairment and left ventricular diastolic dysfunction.

11.
Journal of Medical Biomechanics ; (6): E313-E318, 2017.
Article in Chinese | WPRIM | ID: wpr-803881

ABSTRACT

Objective To determine the hyperelastic constitutive equation of biological soft tissues and study the mechanical responses during the clamping process of biological tissues. Methods The destructive uniaxial tensile test was performed on fresh porcine liver and the uniaxial tension experiment was simulated in ABAQUS. The hyperelastic constitutive equation of porcine liver was determined by comparing the simulation results with the experimental data. Based on this equation, the sharp teeth-shaped and wave-shaped chucks were used to simulate the clamping process. Results The simulation results of the tensile experiment with the 4th-order Ogden model were in good agreement with the experimental data. The results of tissue clamping simulation showed that stress concentration was more likely to occur when the sharp teeth-shaped chuck was adopted. Conclusions The 4th-order Ogden model can be used to describe the hyperelasticity of porcine liver and determine the relevant para-meters. Using the sharp-shaped chuck is more likely to cause tissue clamping damage, and there is a linear relationship between tissue stress and clamping feed distance. These research findings provide references for the design of surgical clamp.

12.
Article in Chinese | WPRIM | ID: wpr-230951

ABSTRACT

To observe the protective effects of Lycium barbarum polysaccharides (LBP) on cerebral ischemia reperfusion injury in mice and explore its mechanism. Common carotid artery thread was used to cause middle cerebral artery ischemia, and the thread was taken out after 2 h ischemia to achieve cerebral ischemia reperfusion injury in mice. Therefore, the transient middle cerebral artery occlusion (tMCAO) models were established to observe the effects of LBP (25,50, 100 mg•kg⁻¹) on neurological outcome, infarct size and water contents. HE staining was used to observe its effects on neurocytes of cerebral tissues in mice. Western blotting was used to evaluate the protein expression levels of NF-κB p65. ELISA was used to evaluate the levels of TNF-α, IL-6 and IL-1β in the serum. According to the results, LBP markedly improved neurologic deficits, and decreased infarct size and water contents at 24 h after reperfusion in mice. Pathological section of brain tissues also proved its protective effects on neurocytes. Western blot analysis indicated that LBP markedly down-regulated the protein level of NF-κB p65. ELISA indicated that LBP decreased the levels of TNF-α, IL-6 and IL-1β in the serum 24 h after reperfusion.In conclusion, LBP has protective effects on cerebral ischemia reperfusion injury in mice, and this effect may be associated with inhibiting NF-κB and inflammatory reactions.

13.
Article in Chinese | WPRIM | ID: wpr-668633

ABSTRACT

This paper reviews the current mobile business research hotspots,and explores the influencing fac-tors of the users' initial trust in the mobile medical background. For the first time,the TAM model is integrated into this study to explore the current situation. At the same time, we introduce the research scope and dimension of the perceived reputation in this context by combining the retreat avoidance and the related group variables in China. The trust mechanism was used to construct the research model on mobile medical care. A questionnaire survey and struc-tural equation model validation served to find out that product participation, the relevant groups, the perception of hospital and doctor's reputation and the security system have a significant impact on the user's initial trust in the mo-bile medical channel. The study results suggest that the mobile medical platform Interaction perfects the whole chan-nel service and at the same time does a good job of the platform itself.

14.
Military Medical Sciences ; (12): 711-713, 2017.
Article in Chinese | WPRIM | ID: wpr-665690

ABSTRACT

Objective To analyze and evaluate the blood preservation effect of two types of blood transportation boxes under field conditions in extreme cold regions of the plain.Methods Before experiment,the hematocrit,hemoglobin(Hb) and free Hb content,Na+and K +of samples of suspended red blood cells(RBCs)were detected and the sterility test was conducted.One of the two boxes was a field blood transportation box that served as control, while the other was made of phase-changed material of two types—1#and 2#.The suspended RBC samples were preserved in the low-temperature blood refrigerators and the two types of boxes respectively and transported under outdoor environment temperatures of -20 --5℃for 2 h.After that,the changes in the blood storage device temperature were recorded after 0,12,24,36,48 and 60 h.Results The suspended RBC preservation time in the field blood transportation box was 42 h vs 58 h in the blood transportation box made of phase-changed material.But their hemolysis rate,Na+,K+and the sterility test all were up to national standards.Conclusion In the extreme cold regions of the plain, the blood transportation box made of phase-changed material can be used to preserve suspended RBCs.

15.
Article in Chinese | WPRIM | ID: wpr-663894

ABSTRACT

Objective To explore the effect of metformin on learning and memory ability and hippocampal tissue structure in mice during aging induced by D-galactose, and the possible underling mechanisms. Methods Twenty-four SPF 7-month-old female ICR mice were randomly divided into three groups. Mice of the aging group and aging+metformin group were given subcutaneous injection with D-galactose on the back to induce senescence, and given intragastric gavage with 0. 9% saline or metformin. Mice of the control group were treated with 0. 9% saline. All treatments lasted for 16 weeks. The body weight and food intake were monitored, learning and memory ability and motor function were tested by Mirros water maze and shuttle box tests, HE staining was used to observe the pathology of hippocampus in the mice, and the levels of glutathione (glutathione, GSH) in hippocampus of mice were detected by colorimetry. Results Compared with the aging group, the aging+meformin group showed diverse differences:the body weight was decreased (P < 0. 05), the escape latency and swimming distance were decreased ( P < 0. 01 or P < 0. 05 ) , the swimming time in the target quadrant was prolonged (P < 0. 05) and swimming speed was accelerated (P < 0. 05) in the Morris water maze test. The numbers of active avoidance response were markedly increased in shuttle box test ( P < 0. 05 ) . The neurons with nuclear condensation and deep staining were obviously decreased in the dentate gyrus of hippocampus, however the GSH level was significantly increased ( P < 0. 05 ) . Conclusions Metformin can delay the decline of learning and memory ability, maintain the normal structure of hippocampus during the aging process in mice, which may be related to the reduction of body weight and enhancement of antioxidant levels in the hippocampus.

16.
Article in Chinese | WPRIM | ID: wpr-661021

ABSTRACT

Objective To investigate the mineralization impact of fibroblast growth factor receptors 1 dominant negative (FGFR1DN) on osteogenic induction culture of bone marrow stromal cells (BMSCs).Methods The 3rd generation BMSCs were divided into 4 equal groups (n =24).FGFR1-DN group was transfected by pcDNA3.1 (+)-FGFR1DN,FGFRI group by pcDNA3.1 (+)-FRFR1,blank load group by pcDNA3.1 (+)-blank vehicle and non-transtection group by nothing.After successful transfection was confirmed when the cells were in the logarithmic phase,osteogenic induction culture was conducted continuously for 21 days.Mineralized nodule formation was observed by alizarin red staining.The amount of mineralized material was calculated according to the standard curve of alizarin red concentration.Results Continuous osteogenic induction for 21 days showed on the bottom of the hole visible round opaque calcified nodules after alizarin red staining.The BMSCs in the FGFR1-DN group induced in the logarithmic growth phase by osteoblasts exhibited significantly increased osteogenic capacity while those in the FGFR1 group displayed diminished osteogenic capacity.The concentration of alizarin red was the highest in the FGFRI-DN group (1.33 ±0.19),the lowest in the FGFRI group (1.00 ± 1.17),and moderate in the blank load group (1.20 ± 0.16) and non-transfection group (1.17 ± 0.17),showing significant between-group differences (P < O.05).Conclusions FGFR1-DN can promote cell proliferation in the early differentiation of BMSCs and also mineralization of osteoblasts in bone induction culture after the logarithmic growth phase.This may provide a hint for local gene treatment of bone defects.

17.
China Journal of Endoscopy ; (12): 47-52, 2017.
Article in Chinese | WPRIM | ID: wpr-660975

ABSTRACT

Objective To observe the changes of esophageal pressure in patients with achalasia (AC) before and after peroral endoscopic myotomy (POEM), and to explore the value of high-resolution manometry (HRM) in evaluating the efficacy of POEM. Methods 38 cases were diagnosed as achalasia and treated with POEM from August 2015 to November 2016. Upper esophageal sphincter resting pressure (UESP), lower esophageal sphincter resting pressure (LESP), esophageal sphincter 4s integrated relaxation pressure (4sIRP) were detected by HRM before operation, at 1 week, 1 month after operation to evaluate the efficacy. Results A total of 38 patients with AC received POEM. At one week, one month after operation, the UESP were (45.34 ± 26.52) mmHg, (41.27 ± 20.09) mmHg, compared with that of before operation (49.58 ± 26.47) mmHg and the differences were not statistically significant. The differences in LESP, 4sIRP between before operation and at one week, one month after operation were statistically significant, P < 0.05. The differences in UESP, LESP and 4sIRP between 1 week and 1 month after operation were not statistically significant. Conclusion The pressure of lower esophageal sphincter was significantly reduced and the dysphagia was improved. HRM might play an important role in the assessment of AC received POEM.

18.
Article in Chinese | WPRIM | ID: wpr-659887

ABSTRACT

Objective To study and analyze the clinical effect of case care management in patients with schizophrenia treated with Paliperidone palmitate. Methods 90 patients with schizophrenia in the third People's hospital of Huzhou were selected as the subjects. The patients in the experimental group and the control group were treated with Paliperidone palmitate for 12 months. The control group was given routine nursing, the experimental group was given case nursing management, health education, professional psychological counseling, paying close attention to the clinical symptoms of patients, seminars, encouraging patients to contact the society, regular analysis of problems. PANSS score and MARS score and other clinical indicators of the experimental group and the control group of patients were compared and analyzed. Results After treatment, the medication compliance score of the experimental group was (7.32±0.34) points, significantly higher than that (5.12±0.23) points of the control group with statistical significance (P<0.05). The score of PANSS in the control group was (42.90±3.87) points, and the score of PANSS in the experimental group was (36.78±3.32) points, and the difference between the two groups was statistically significant (P<0.05). Conclusion nursing case management plus Paliperidone palmitate treatment in patients with schizophrenia has good clinical curative effect, could significantly improve the clinical symptoms of patients, help patients recovery, has clinical application significance.

19.
Article in Chinese | WPRIM | ID: wpr-658194

ABSTRACT

Objective To investigate the mineralization impact of fibroblast growth factor receptors 1 dominant negative (FGFR1DN) on osteogenic induction culture of bone marrow stromal cells (BMSCs).Methods The 3rd generation BMSCs were divided into 4 equal groups (n =24).FGFR1-DN group was transfected by pcDNA3.1 (+)-FGFR1DN,FGFRI group by pcDNA3.1 (+)-FRFR1,blank load group by pcDNA3.1 (+)-blank vehicle and non-transtection group by nothing.After successful transfection was confirmed when the cells were in the logarithmic phase,osteogenic induction culture was conducted continuously for 21 days.Mineralized nodule formation was observed by alizarin red staining.The amount of mineralized material was calculated according to the standard curve of alizarin red concentration.Results Continuous osteogenic induction for 21 days showed on the bottom of the hole visible round opaque calcified nodules after alizarin red staining.The BMSCs in the FGFR1-DN group induced in the logarithmic growth phase by osteoblasts exhibited significantly increased osteogenic capacity while those in the FGFR1 group displayed diminished osteogenic capacity.The concentration of alizarin red was the highest in the FGFRI-DN group (1.33 ±0.19),the lowest in the FGFRI group (1.00 ± 1.17),and moderate in the blank load group (1.20 ± 0.16) and non-transfection group (1.17 ± 0.17),showing significant between-group differences (P < O.05).Conclusions FGFR1-DN can promote cell proliferation in the early differentiation of BMSCs and also mineralization of osteoblasts in bone induction culture after the logarithmic growth phase.This may provide a hint for local gene treatment of bone defects.

20.
China Journal of Endoscopy ; (12): 47-52, 2017.
Article in Chinese | WPRIM | ID: wpr-658172

ABSTRACT

Objective To observe the changes of esophageal pressure in patients with achalasia (AC) before and after peroral endoscopic myotomy (POEM), and to explore the value of high-resolution manometry (HRM) in evaluating the efficacy of POEM. Methods 38 cases were diagnosed as achalasia and treated with POEM from August 2015 to November 2016. Upper esophageal sphincter resting pressure (UESP), lower esophageal sphincter resting pressure (LESP), esophageal sphincter 4s integrated relaxation pressure (4sIRP) were detected by HRM before operation, at 1 week, 1 month after operation to evaluate the efficacy. Results A total of 38 patients with AC received POEM. At one week, one month after operation, the UESP were (45.34 ± 26.52) mmHg, (41.27 ± 20.09) mmHg, compared with that of before operation (49.58 ± 26.47) mmHg and the differences were not statistically significant. The differences in LESP, 4sIRP between before operation and at one week, one month after operation were statistically significant, P < 0.05. The differences in UESP, LESP and 4sIRP between 1 week and 1 month after operation were not statistically significant. Conclusion The pressure of lower esophageal sphincter was significantly reduced and the dysphagia was improved. HRM might play an important role in the assessment of AC received POEM.

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