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1.
Article in Chinese | WPRIM | ID: wpr-928206

ABSTRACT

Autophagy is a programmed cell degradation process that is involved in a variety of physiological and pathological processes including malignant tumors. Abnormal induction of autophagy plays a key role in the development of hepatocellular carcinoma (HCC). We established a prognosis prediction model for hepatocellular carcinoma based on autophagy related genes. Two hundred and four differentially expressed autophagy related genes and basic information and clinical characteristics of 377 registered hepatocellular carcinoma patients were retrieved from the cancer genome atlas database. Cox risk regression analysis was used to identify autophagy-related genes associated with survival, and a prognostic model was constructed based on this. A total of 64 differentially expressed autophagy related genes were identified in hepatocellular carcinoma patients. Five risk factors related to the prognosis of hepatocellular carcinoma patients were determined by univariate and multivariate Cox regression analysis, including TMEM74, BIRC5, SQSTM1, CAPN10 and HSPB8. Age, gender, tumor grade and stage, and risk score were included as variables in multivariate Cox regression analysis. The results showed that risk score was an independent prognostic risk factor for patients with hepatocellular carcinoma ( HR = 1.475, 95% CI = 1.280-1.699, P < 0.001). In addition, the area under the curve of the prognostic risk model was 0.739, indicating that the model had a high accuracy in predicting the prognosis of hepatocellular carcinoma. The results suggest that the new prognostic risk model for hepatocellular carcinoma, established by combining the molecular characteristics and clinical parameters of patients, can effectively predict the prognosis of patients.


Subject(s)
Autophagy/genetics , Biomarkers, Tumor/genetics , Carcinoma, Hepatocellular/pathology , Humans , Liver Neoplasms/pathology , Membrane Proteins/genetics , Prognosis
2.
Journal of Clinical Hepatology ; (12): 2167-2170, 2021.
Article in Chinese | WPRIM | ID: wpr-904861

ABSTRACT

Objective To investigate the influence of the concept of collaborative innovation on the construction of a scientific research system for regional liver pathology center from multiple perspectives, and to explore its application significance. Methods A total of 1246 liver biopsy specimens which were examined in Department of Pathology in Wuxi Second People's Hospital and Wuxi Fifth People's Hospital from January 2014 to September 2020 were collected, among which the specimens examined before the founding of the regional liver pathology center were collected as nI group, and those examined after the founding of the center were collected as nII group. According to the examination time of the first, second, or third year after founding, the nII group was further divided into nIIa group, nIIb group, and nIIc group. A multi-perspective analysis was performed based on five indices, i.e., number of liver biopsy specimens, growth rate of liver biopsy specimens, growth rate of internal collaborative departments, growth rate of external collaborative units, and evaluation of the collaborative and innovative scientific research system. Results The growth rate of liver biopsy specimens in the nI group was 15.51%; after the founding of the regional liver pathology center, the highest growth rate of 187.76% was observed in the nIIa group, and a relatively stable growth rate of 76.35% was observed in the nIIb group. There was a gradual increase in the number of specimens in internal collaborative departments, with a growth rate of 50% in the nIIa group and 83.33% in the nIIb group, while the number of external collaborative units increased steadily, with a growth rate of 100% in the nIIa group and 50% in the nIIb group. The nII group showed a diversified increasing trend in the evaluation indices of the scientific research system. Conclusion It is a new perspective for the construction of regional disciplinary scientific research system to grasp the opportunity of policy-supported development and innovate the effective cooperation mode between internal departments and external units under the concept of collaborative innovation.

3.
Article in Chinese | WPRIM | ID: wpr-881485

ABSTRACT

Objective:To analyze and compare the differences between the epidemiological data and clinical indicators of confirmed and suspected undiagnosed cases of COVID-19 in Changning District, Shanghai. Methods:A retrospective comparative study was conducted. We included 20 confirmed and 34 suspected but undiagnosed COVID-19 cases from January 20 to February 29, 2020. We analyzed the differences in epidemiological history, early clinical symptoms, blood routine indicators, and clinical imaging characteristics between the two groups. Results:The epidemic status of COVID-19 in Changning District of Shanghai was mainly imported, and most cases were promptly confirmed. Early clinical symptoms of confirmed and suspected undiagnosed cases often manifested as respiratory symptoms such as fever and dry cough. Compared with the confirmed cases, the cell counts of leukocytes, eosinophils, and neutrophils in suspected undiagnosed cases were significantly higher. Also, the concentration of serum C-reactive protein in suspected cases was higher than that in confirmed cases (P=0.230). The clinical imaging manifestations of confirmed cases were mainly ground glass opacity (GGO) scattered in both lung leaves, while the suspected undiagnosed cases mainly manifested as plain patch opacity, and the distribution of GGO was more irregular. Conclusion:There exists significant difference in blood routine indicators and clinical imaging features between confirmed and suspected cases of COVID-19.

4.
Article in Chinese | WPRIM | ID: wpr-881482

ABSTRACT

Objective:To assess the current situation of information security literacy, and determine its factors. Methods:Literature review and Delphi method were used to design a questionnaire on information security literacy. All users of disease control information system in Jinshan District,Shanghai were investigated. The questionnaire included information security related knowledge, awareness, role cognition, and behavior. Results:The overall proportion of information security literacy was determined to be 7.98%. Information security knowledge, awareness, role cognition, and behavior was identified in 17.18%, 37.42%, 62.58%, and 38.04% of the users, respectively. Sex, age and type of information system account were associated with the information security literacy. Conclusion:Information security literacy remains low in Jinshan District, which may not meet the current requirement in work. Particularly, information security behavior is at risk, which warrants further improvement in the information security management.

5.
Article in Chinese | WPRIM | ID: wpr-881476

ABSTRACT

Objective:To determine the level of anti-measles antibody in healthy population aged 1-14 years old in Shanghai, and project the risk of measles incidence in adult population in the future, which may provide evidence for enhancing the measles immunization strategy. Methods:A cross-sectional study was conducted to collect the serum of healthy people aged 1-14 years old in an urban district hospital in Shanghai. Serum concentration of anti-measles IgG antibody was examined by enzyme-linked immunosorbent assay (ELISA) and dynamics of IgG antibody was compared. Results:The prevalence of anti-measles antibody in healthy people aged 1-14 years old was determined to be 95.29%, and the antibody protection rate was 61.86%. There was no significant difference in the distribution of antibody between men and women (P>0.05). However, there was statistical difference in the distribution of antibody among different age groups (P<0.05), in which the antibody showed a decreasing trend with age, especially after the age of 10 years. The lowest prevalence of anti-measles antibody and protection rate were observed in the population aged 13-14 years old, which were 87.41% and 22.96%, respectively. The GMT was 2.667 1 (equal to the concentration of anti-measles IgG antibody being 464.62 mIU/mL). According to the fitting model Ŷ =3.217-0.04X(R2=0.943,P<0.05), the antibody protection rate was projected to decrease to zero in the population aged 19-20 years old, whereas the anti-measles antibody was to zero in the population aged 29-30 years old . Conclusion:As there is almost no natural infection of measles,the anti-measles antibody after measles vaccine immunization showed a linearly decreasing trend with age after measles immunization.It is recommended that people aged 10-15 years should be administered intensive immunization for prevention of adults measles,which could be incorporated into current measles immunization strategies.

6.
Article in Chinese | WPRIM | ID: wpr-828132

ABSTRACT

Since the emergence of novel coronavirus pneumonia in late 2019, it has quickly spread to many countries and regions around the world, causing a significant impact on human beings and society, posing a great threat to the global public health system. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was highly infectious, and some complications emerged rapidly in some patients, including acute respiratory distress syndrome, and multiple organ failure. The virus could trigger a series of immune responses, which might lead to excessive immune activation, thereby bringing about the immune system imbalance of the body. Up to now, there was no specific antiviral drug, and we conjectured that immunomodulatory therapy might play an essential part in the treatment of coronavirus disease 2019 (COVID-19) as adjuvant therapy. Therefore, we analyzed the possible mechanism of immune imbalance caused by the new coronavirus, and summarized the immunotherapeutic means of COVID-19 based on the mechanisms, to provide some reference for follow-up research and clinical prevention and treatment of COVID-19.


Subject(s)
Betacoronavirus , Coronavirus Infections , Humans , Pandemics , Pneumonia, Viral
7.
Article in English | WPRIM | ID: wpr-880313

ABSTRACT

BACKGROUND@#Decreased heart rate variability (HRV) is a predictor of autonomic system dysfunction, and is considered as a potential mechanism of increased risk of cardiovascular disease (CVD) induced by exposure to particulate matter less than 2.5 μm in diameter (PM@*METHODS@#An updated systematic review and meta-analysis of panel studies till November 1, 2019 was conducted to evaluate the acute effect of exposure to ambient PM@*RESULTS@#A total of 33 panel studies were included in our meta-analysis, with 16 studies conducted in North America, 12 studies in Asia, and 5 studies in Europe. The pooled results showed a 10 μg/m@*CONCLUSION@#Short-term exposure to PM


Subject(s)
Adult , Aged , Aged, 80 and over , Air Pollutants/analysis , Air Pollution/analysis , Female , Heart Rate/drug effects , Humans , Male , Middle Aged , Particulate Matter/analysis , Young Adult
8.
Article in Chinese | WPRIM | ID: wpr-868465

ABSTRACT

Objective:To explore the relationship between semi-quantitative parameters of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and long-term prognosis of locally advanced nasopharyngeal carcinoma, and to find prognostic indicators from non-invasive images of locally advanced nasopharyngeal carcinoma.Methods:Data were collected from January 2011 to January 2012 via a prospective clinical trial with locally advanced nasopharyngeal carcinoma. Clinical information was from 71 patients who completed the treatment plan with long-term follow-ups and UICC 2010 stage Ⅲ, Ⅳ A, Ⅳ B. The patients received three cycles of Taxotere-Platinol-Fluorouracil (TPF) regimen chrono-chemotherapy, followed by two cycles of concurrent paclitaxel chemotherapy with intensity-modulated radiotherapy (IMRT). DCE-MRI examination was performed before induction chemotherapy to obtain DCE-MRI related semi-quantitative parameters. Correlation analysis was conducted between DCE-MRI related semi-quantitative parameters and short-term efficacy of nasopharyngeal lesions after concurrent radiotherapy and chemotherapy. Results:Of all 77 patients, 71 completed treatment and were followed up from 9 to 86 months, with a median follow-up of 77 months, with 80.2% and 67.6% in 3- and 5-year OS, 73.2% and 60.5% in 3- and 5-year PFS, respectively. Evaluation of short-term efficacy of nasopharyngeal lesions after concurrent chemoradiotherapy: the difference in tissue arrival time of contrast agent between complete response (CR) group and partial response (PR) group was statistically significant ( t=0.537, P<0.05). Univariate survival analysis found that OS ( χ2=3.982, P<0.05) and PFS ( χ2=4.019, P<0.05) in the group with short contrast arrival time were significantly higher than those in the group with long contrast arrival time. OS ( χ2=7.593, P<0.05) and PFS ( χ2=5.624, P<0.05) of patients aged over 45 years were significantly lower than those aged less than 45 years. Cox multivariate regression model showed that advanced clinical stage (stage Ⅳ A, Ⅳ B) ( P=0.048) and age≥45 years ( P=0.031) were independent prognostic factors of OS in patients with nasopharyngeal carcinoma. Long arrival time of contrast agent ( P=0.018), age≥45 years ( P=0.004), advanced N(2-3) stage ( P=0.032) and enhancement peak<3 000 ( P=0.005) were independent prognostic factors of PFS in patients with nasopharyngeal carcinoma. Conclusions:The arrival time of the contrast agent in DCE-MRI may be a reliable prognostic factor for locally advanced nasopharyngeal carcinoma.

9.
Chinese Journal of Radiology ; (12): 1095-1100, 2020.
Article in Chinese | WPRIM | ID: wpr-868379

ABSTRACT

Objective:To investigate the correlation of age with diffusion tensor imaging (DTI) values as fractional anisotropy (FA), apparent diffusion coefficient (ADC) and contrast to noise ratio (CNR) of three-dimensional nerve-sheath signal increased with inked rest-tissue rapid acquisition of relaxation imaging (3D SHINKEI) of the brachial plexus in normal adults.Methods:A total of 54 adult healthy volunteers and 6 patients with Guillain-Barre syndrome were prospectively enrolled from October 2018 to April 2019. Healthy volunteers were divided into 3 groups according to age as 21-40 years old group ( n=20), 41-60 years old group ( n=20), and ≥61 years old group ( n=14). All of them underwent MRI examination of the brachial plexus, including DTI and 3D SHINKEI sequences. The average FA and ADC values of the brachial plexus were measured and calculated through the fusion of DTI and 3D SHINKEI by 2 physicians independently. The contrast to noise ratio (CNR) of brachial plexus nerve was measured in 3D SHINKEI sequence images. Intraclass correlation efficient (ICC) was used to analyze the consistency between the two physicians. A simple linear regression model and Pearson correlation analysis were used to detect the correlation between FA, ADC, CNR and age. One-way analysis of variance was used to compare the differences of FA, ADC and CNR in different age groups. The FA and ADC values in different genders were compared by independent sample t-test. Results:Inter-observer agreements of the 2 physicians were good for FA and ADC values with ICC values of 0.811 and 0.901, respectively. For different groups, FA values were 0.397±0.023, 0.368±0.023, and 0.334±0.018 and ADC values were (1.376±0.072) × 10 -3 mm 2/s, (1.466±0.086) × 10 -3 mm 2/s, (1.486±0.080) × 10 -3 mm 2/s, for 21-40, 41-60, and ≥61 years old groups, respectively with statistical significant difference ( F=25.311, P<0.001; F=9.948, P<0.001). The CNR of the brachial plexus were 510.583±192.846, 502.581±128.821, and 426.782±113.648 for 21-40, 41-60, and ≥61 years old group without statistical difference ( F=1.429, P=0.249). The FA value of brachial plexus was highly negatively correlated with age ( r=-0.745, P<0.001), while the ADC value was moderately positively correlated with age ( r=0.596, P<0.001). The CNR of 3D SHINKEI sequence was negatively correlated with age ( r=-0.292, P=0.033). There was no statistically significant difference in brachial plexus FA and ADC values between male and female subjects ( t=1.496, P=0.141; t=-1.557, P=0.126). The FA value of Guillain-Barre syndrome patients was lower than that of healthy volanteers in the same age group ( t=6.129, P<0.001), and the ADC value had no statistical diference ( t=-1.335, P=0.186). Conclusion:The values of FA, ADC and CNR of brachial plexus in normal adults change with age. Among them, FA value is more significant.

10.
Chinese Journal of Radiology ; (12): 235-240, 2020.
Article in Chinese | WPRIM | ID: wpr-868272

ABSTRACT

Objective:To evaluate alterations of periventricular pseudocysts (PVPC) on MRI before and after birth, and to assess the prognosis.Methods:We retrospectively analyzed the data of 67 cases that were diagnosed with PVPC on prenatal MRI, of which 24 cases were lost to follow-up, 2 died after birth. A total of 41 surviving fetuses were included in this prognosis study. The gestational ages in this group were between 23 and 39 weeks, with an average of (33±3) weeks.All the subjects underwent brain MRI examinations and Gesell Developmental Scale (GDS) testing between 0-3 years of age. According to the location of cysts and with or without other intracranial and extracranial malformations (dilated ventricles orcerebella medulla, hypoxic-ischemic encephalopathy, TORCH virus infection, corporal hypoplasia, chromosomal malformations and nodular sclerosis) , the patients were divided into four groups: isolated connatal cysts, connatal cysts with additional findings,isolated subependymal pseudocysts, and subependymal pseudocysts with additional findings.The MR images were independently reviewed by two radiologists blinded to the clinical information. Intraclass correlation efficient (ICC) was used to analyze the consistency between the two reviewers.Chi-square test was used to compare the location of cysts (single/bilateral), the number of cyst cavities (single/multi-chamber), and other abnormalities in the connatal cyst group and subependymal cyst group. The mean anteroposterior diameter and mean height of cysts between the connatal cyst group and subependymal cyst group were compared by independent sample t-test.The ANOVA test was used to compare the differences in GDS outcomes among the groups. Multiple comparisons were conducted using the LSD test. Results:Inter-observer agreements between the two radiologists were good for the collected data (all ICC>0.75). Eleven isolated connatal cysts and 7 connatal cysts with additional findings became smaller or disappeared, and all had good prognosis. Of the 14 isolated subependymal cysts, 12 became smaller or disappeared, 2 had no change in size, and 13 had good prognosis. The subependymal cysts with additional findings group included 9 cases: 6 became smaller or disappeared, only 3 showed no apparent changes, and 7 had an abnormal outcome. Subependymal cysts with additional findings were significantly reduced and patients demonstrated significant differences compared with the those with isolated subependymal cysts in the development quotients (DQ) of adaptability, large movements, fine movements, personal social interaction, and language DQ ( P all<0.05). DQ between patients with isolated connatal cysts and isolated subependymal cysts was comparable ( P all>0.05). When associated with additional findings, connatal cysts and subependymal cysts could induce significant different DQ outcome ( P all<0.05). Conclusions:Isolated PVPC usually become smaller or disappeared and have a benign presentation after birth, whereas patients with subependymal cysts with additional findings usually have a poor prognosis. Connatal cysts usually have a good prognosis.

11.
Chinese Journal of Digestion ; (12): 619-625, 2019.
Article in Chinese | WPRIM | ID: wpr-756311

ABSTRACT

Objective To explore the role of microtubule actin cross-linking factor 1 (MACF1) in the metastasis of gastric cancer.Methods From 2009 to 2012,at The First Affiliated Hospital of Zhengzhou University,the paraffin blocks of gastric cancer and normal tissue adjacent to cancer of 107 patients who received radical gastrectomy were collected.The expression of MACF1 in tissues at protein level was detected by immunohistochemical staining.In 2017,at The First Affiliated Hospital of Zhengzhou University,fresh specimens samples of gastric cancer and normal tissue adjacent to cancer of 42 patients who received radical gastrectomy were also collected.The expression of MACF1 at mRNA level was determined by quantitative real-time polymerase chain reaction (PCR).MACF1 knockout gastric cell line was established.The effects of MACF1 on cell migration and invasion were verified by wound-healing test and Transwell assay.The effects of MACF1 on cell microtubule and actin were analyzed by filamentous actin (F-actin) staining.T-test,chi-square test and multivariate analysis were used for statistical analysis.Results The positive expression rate of MACF1 in gastric carcinoma tissues was 71.0% (76/107),which was significantly higher than that of the corresponding normal tissues adjacent to cancer (22.4%,24/107),and the difference was significant (t =4.145,P =0.016).The expression of MACF1 at mRNA level in cancer tissues of 42 patients with gastric cancer was 6.463 ±0.672,which was significantly higher than that of corresponding normal tissue adjacent to cancer (1.727 ± 0.331),and the difference was statistically significant (t =6.326,P < 0.01).The differences in positive expression rate of MACF1 in different tumor infiltration depth,different TNM stage and lymph nodes metastasis were statistically significant (x2 =1.170,7.959 and 5.288;all P < 0.01).The five-year survival rate of patients with high expression of MACF1 was 32.9% (25/76),which was significantly lower than that ofpatients with normal MACF1 expression (64.5%,20/31),and the difference was statistically significant (x2 =25.093,P =0.034).The high expression of MACF1 was an independent prognostic factor affecting overall survival rate in patients with gastric cancer after surgery (hazard ratio (HR) =0.513,95% confidence interval (CI):0.411 to 0.922,P =0.038).The results of wound-healing assay showed that at 24 hour after wound the migration ability of MACF1 knockout AGS-MACF1 / cells was (18.77 ± 3.82) %,which was lower than that of wild type AGS cells ((76.24 ± 5.36) %),and the difference was statistically significant (t =6.249,P =0.014).The migration ability of MACF1 knockout HGC27-MACF1-/-cells was (42.48 ± 7.37)%,which was lower than that of wild type HGC27 cells ((82.35-± 4.28) %),and the difference was statistically significant (t =5.938,P =0.017).The results of Transwell assay indicated that the number of migration cells of MACF1 knockout AGS-MACF1-/-cells was 87.0 ± 11.0,which was less than that of wild type AGS cells (200.0 ± 16.0),and the difference was statistically significant (t =5.820,P =0.028).The number of migration cells of MACF1 knockout HGC27-MACF1-/-cells was 151.0 ± 13.0,which was less than that of wild type HGC27 cells (268.5 ± 20.5),and the difference was statistically significant (t =4.840,P =0.040).The results of F-actin staining demonstrated that the number of actin filaments of MACF1 knockout AGS-MACF1-/-cells was 216.60 ± 18.09,which was less than that of wild type AGS cells (491.30 ± 5.02),and the difference was statistically significant (t =14.630,P =0.005).Conclusions The abnormally high expression of MACF1 in gastric cancer tissues may be correlated with the poor prognosis of patients with gastric cancer.MACF1 promotes the invasion and metastasis of gastric cancer cells by affecting the formation of F-actin and cell skeleton.

12.
Journal of Clinical Hepatology ; (12): 2159-2162, 2019.
Article in Chinese | WPRIM | ID: wpr-778728

ABSTRACT

Hepatitis B virus core-related antigen (HBcrAg) is a new serum biomarker for hepatitis B virus (HBV) and is composed of several antigens encoded by the pre-C/C region gene of HBV, including HBcAg, HBeAg, and P22cr precursor protein. There is a poor correlation between HBcrAg and HBsAg and they cannot replace each other. Serum HBcrAg level can reflect the content and transcriptional activity of cccDNA in hepatocytes of patients with chronic hepatitis B, as well as the transcriptional activity of integrated HBV DNA. In addition, HBcrAg can be used to evaluate the antiviral effect of nucleos(t)ide analogues and pegylated interferon- and predict recurrence risk after withdrawal of nucleos(t)ide analogues and the development risk and recurrence of hepatocellular carcinoma after surgery. Therefore, serum HBcrAg is a promising new serum marker for HBV.

13.
Journal of Clinical Hepatology ; (12): 2137-2139, 2019.
Article in Chinese | WPRIM | ID: wpr-778723

ABSTRACT

The objective of antiviral therapy for chronic hepatitis B is to maximize the long-term inhibition of hepatitis B virus (HBV) replication, reduce hepatocyte inflammation and liver fibrosis, and delay and reduce liver failure, decompensated cirrhosis, hepatocellular carcinoma, and other complications. In addition to serum HBV DNA which has been used for a long time, new biomarkers such as intrahepatic HBV cccDNA, serum HBcrAg, serum HBV RNA, serum HBsAg, and serum anti-HBc are commonly used in recent years to evaluate antiviral response in clinical practice, and their clinical value has attracted more and more attention. Such biomarkers have important clinical significance in monitoring and evaluating antiviral response and predicting the risk of viral rebound after drug withdrawal and the risk of hepatocellular carcinoma.

14.
Acta Physiologica Sinica ; (6): 424-430, 2019.
Article in Chinese | WPRIM | ID: wpr-777171

ABSTRACT

The present study was aimed to investigate the protective effect and anti-inflammation mechanism of astragaloside IV (AST-IV) on cerebral ischemia and reperfusion injury. Following the establishment of cerebral ischemia and reperfusion model in rats by modified suture method, neurological deficit scores and cerebral infarct volume were used to evaluate the pharmacological effect of AST-IV against cerebral ischemia-reperfusion injury. Western blot was used to detect the expression levels of NLRP3, pro-Caspase-1, Caspase-1, pro-IL-1β, IL-1β, pro-IL-18, IL-18, phosphorylated and total nuclear factor kappa B (NF-κB)/p65 protein in the brain tissue. The results showed that compared with model group, the intervention of AST-IV decreased the neurological deficit scores, reduced the cerebral infarct volume, decreased the levels of NLRP3, Caspase-1, pro-IL-1β, IL-1β, pro-IL-18 and IL-18, and inhibited the expression of phosphorylated NF-κB in brain tissue. The results suggest that AST-IV has a protective effect against cerebral ischemia and reperfusion injury, and its mechanism is related to inhibiting the phosphorylation of NF-κB and NLRP3 inflammasome activation.


Subject(s)
Animals , Brain Ischemia , Drug Therapy , Infarction, Middle Cerebral Artery , Drug Therapy , Inflammasomes , Metabolism , NF-kappa B , Metabolism , NLR Family, Pyrin Domain-Containing 3 Protein , Metabolism , Phosphorylation , Rats , Rats, Sprague-Dawley , Reperfusion Injury , Drug Therapy , Saponins , Pharmacology , Triterpenes , Pharmacology
15.
Article in Chinese | WPRIM | ID: wpr-775934

ABSTRACT

OBJECTIVE@#To observe the effects of acupuncture at "Baihui" (GV 20) and "Shenshu" (BL 23) on the ultrastructure of hippocampal dentate gyrus in rats with Alzheimer's disease.@*METHODS@#Forty SPF Wistar male rats were randomly divided into a normal group, a sham operation group, a model group and an acupuncture group, 10 rats in each one. The rats in the model group and the acupuncture group were treated with injection of 5 μL Aβ at bilateral hippocampus, while the rats in the sham operation group were treated with injection of 5 μL 0.9% NaCl. Three days after modeling, the rats in the acupuncture group were treated with acupuncture at "Baihui" (GV 20) and "Shenshu" (BL 23) for 20 min, once a day, six treatments constituted a course, and totally two courses were given with an interval of 1 day between courses. The rats in the other groups received normal diet and no treatment was given. Before modeling, four days after modeling and after treatment, water maze test was performed to observe the escape latency and the number of crossing platforms. The hippocampal dentate gyrus was collected and transmission electron microscope was applied to observe the ultrastructure changes of neurons and astrocytes.@*RESULTS@#①Four days after modeling, compared with the normal group and the sham operation group, the escape latency was significantly prolonged and the number of crossing platforms was reduced in the model group (all <0.01); after treatment, compared with the model group, the escape latency was significantly reduced and the number of crossing platforms was increased in the acupuncture group (both <0.01). ②In the normal group and the sham operation group, the morphology of neurons and astrocytes was intact, the nuclear and membrane structure were clear, and the morphology of organelles such as mitochondria, endoplasmic reticulum and lysosomes was normal. In the model group, the morphology of neurons was irregular, the nucleus was severely constricted with edema in the cytoplasm, the color of heterochromatin was deepened, the endoplasmic reticulum was expanded, the granulation was removed and the number of mitochondria was decreased, even with malformed-like change in mitochondrial cristae; there was severe edema around astrocytes, few organelles in the cytoplasm, severe swelling of mitochondria and mild expansion of the endoplasmic reticulum. In the acupuncture group, the edema of the neuron and astrocytes was still evident, and the mitochondrial was mildly swollen but relieved compared with that in the model group, and there were no obvious abnormalities in neuronal endoplasmic reticulum and ribosomes.@*CONCLUSION@#Acupuncture could improve the ultrastructure of neurons and astrocytes in the hippocampal dentate gyrus in rats with Alzheimer's disease induced by Aβ.


Subject(s)
Acupuncture Therapy , Alzheimer Disease , Amyloid beta-Peptides , Animals , Astrocytes , Dentate Gyrus , Hippocampus , Male , Neurons , Peptide Fragments , Rats , Rats, Sprague-Dawley , Rats, Wistar
16.
Article in Chinese | WPRIM | ID: wpr-775921

ABSTRACT

OBJECTIVE@#To observe the clinical therapeutic effects of auricular gold-needle therapy on chronic fatigue syndrome of deficiency constitution and explore its potential mechanism.@*METHODS@#A total of 120 patients were randomized into an auricular gold-needle therapy group, an auricular point pressure therapy group and a Chinese herb group, 40 cases in each one. Additionally, a health control group (40 cases) was set up, without any intervention. In the auricular gold-needle therapy group, the gold needle was used to stimulate the auricular points on one side and the cowherb seed pressure therapy on the other side. In the auricular point pressure therapy group, the cowherb seed pressure therapy was adopted only on one side. The auricular points were shen (CO), xin (CO), fei (CO), pizhixia (AT), etc. in the two groups. The auricular points on both sides were used alternatively. The treatment was given once a week, 4 treatments as one course and the consecutive 3 courses of treatment were required. In the Chinese herb group, was prescribed for oral administration, 6 g, twice a day, the medication for 1 month was as one session and the consecutive 3 sessions of medication were required. Before and after treatment, separately, the clinical symptom score, the levels of the serum immunoglobulins, i.e. IgA, IgG and IgM were observed in the patients of the three groups. The therapeutic effects were evaluated in the three groups.@*RESULTS@#The total effective rate was 90.0% (36/40) in the auricular gold-needle therapy group, better than 80.0% (32/40) in the auricular point pressure therapy group and 82.5% (33/40) in the Chinese herb group (both <0.05). Before treatment, the clinical symptom scores of the patients in the three groups were obviously higher than the health control group (all <0.001). After treatment, the symptom scores were all reduced as compared the scores before treatment in the three groups (all <0.001) and the symptom scores in the auricular gold-needle therapy group were better than the auricular point pressure therapy group and the Chinese herb group (both <0.01). Before treatment, the levels of serum IgA, IgG and IgM of the patients in the three groups were lower than the health control group (all <0.001). The levels were all improved after treatment in the three groups (all <0.01), and the levels in the auricular gold-needle therapy group was better than the auricular point pressure therapy group and the Chinese herb group (all <0.05).@*CONCLUSION@#The auricular gold-needle therapy achieves the significant therapeutic effects on chronic fatigue syndrome of deficiency constitution and its mechanism is probably related to the regulation of immune function.


Subject(s)
Acupuncture Therapy , Fatigue Syndrome, Chronic , Gold , Humans , Qi , Treatment Outcome
17.
Journal of Medical Postgraduates ; (12): 248-252, 2019.
Article in Chinese | WPRIM | ID: wpr-818221

ABSTRACT

Objective Studies are rarely reported on the factors influencing prognosis of surgically resected lung adenocarcinoma with a micropapillary pattern (LAC-MPP). This study aimed to explore the clinicopathological characteristics and risk factors of surgically resected LAC-MPP. Methods We retrospectively analyzed 384 cases of LAC treated in Henan Cancer Hospital between June 2015 and December 2017, which were classified into an MPP group (n = 82) and a non-MPP control group (n = 302) according to the results of postoperative pathology. We determined the expression of the fusion protein anaplastic lymphoma kinase (ALK), analyzed its association with the clinicopathological features of LAC-MPP, and explored the risk factors of postoperative MPP. Results Compared with the non-MPP group, the LAC-MPP patients showed a significantly higher expression of ALK (0.03% vs 12.20%, P < 0.05), rate of bronchial invasion (30.80% vs 48.78%, P < 0.05) and vascular tumor thrombus (0.99% vs 25.61%, P < 0.05), but a lower mutation rate of the epidermal growth factor receptor (EGFR) (64.24% vs 51.22%, P < 0.05). Multivariate logistic regression analysis revealed that the expression of ALK, vascular tumor thrombus, and age were significantly associated with the risk of postoperative MPP. Conclusion There is a high incidence rate of ALK expression in LAC-MPP patients after operation, which may provide some new ideas for the clinical treatment of the disease. Special attention should be paid to the expression of the ALK fusion protein and vascular tumor thrombus, and age in patients with LAC-MPP after operation.

18.
Chinese Journal of School Health ; (12): 1173-1176, 2019.
Article in Chinese | WPRIM | ID: wpr-817589

ABSTRACT

Objective@#To analyze the detection rate of sleep problems such as sleep delay and deficiency in preschool children in the middle and lower reaches of the Yangtze River in China,and to provide the reference for the standard of sleeping mode among preschool students.@*Methods@#From October to November 2017, a questionnaire survey was conducted among 27 200 preschool children in 11 cities in Hubei, Anhui and Jiangsu provinces in the middle and lower reaches of the Yangtze River in China. Epidemiology of sleep delays, deficiencies and sleep patterns in preschool children was described.@*Results@#The detection rate of sleep problems in preschool children in the middle and lower reaches of the Yangtze River was 15.3%. Taking the length of sleep and bedtime as the main analysis points, it was found that the average sleeping time point of each age group was 21:31, and the detection rate of bedtime delay was 86.5%. The average length of sleep was (10.60±1.12) hours. The detection rate of sleep deprivation in preschool children was 15.7%. Sleep delay was positively correlated with girls, age increase and parents’ higher educational level (P<0.05), and negatively correlated with living in the city, non-only child and bedroom without TV (P<0.01) .The detection rate of sleep deprivation was positively correlated with children of high age group (4yearold group:OR=1.32,95%CI=1.19-1.46;5-year-old group:OR=2.10,95%CI=1.91-2.32;6-year-old group:OR=2.47,95%CI=2.20-2.77)(P<0.01), and negatively correlated with no TV in bedroom (OR=0.91,95%CI=0.84-0.98) and no light in sleep (OR=0.87,95%CI=0.78-0.97)(P<0.05).@*Conclusion@#Preschool children sleep delay and sleep deprivation and other sleep problems are more prominent, affected by family environment and other factors.

19.
Chinese Journal of Hepatology ; (12): 483-486, 2019.
Article in Chinese | WPRIM | ID: wpr-810752

ABSTRACT

Liver cirrhosis is a common kind of chronic liver diseases, with the development of diseases; some patients may gradually develop precancerous lesions, or even progress to hepatocellular carcinoma (HCC). Precancerous lesions of the liver mainly include dysplastic foci (DF) and dysplastic nodules (DN), and most of it occurs on the basis of liver cirrhosis. Thus, recognition of precancerous lesions and liver cirrhosis through screening, combined with imaging and pathological features will identity the nature of nodules in early stage cirrhosis and HCC, and thereby will help to improve the diagnosis rate and clinical prognosis.

20.
Article in Chinese | WPRIM | ID: wpr-816050

ABSTRACT

Non-hepatotropic viruses such as Epstein-Barr virus(EBV) has high infection rate in the population with biological characteristics of infective-latent-activation. EBV-related diseases including infectious mononucleosis(IM), chronic active EBV infection(CAEBV), EBV-related tumors and other diseases are complex and diverse, and all these can lead to varying degrees of liver damage.CAEBV can induce hepatitis(chronic active Epstein-Barr virus hepatitis, CAEBVH) or even liver failure. The prognosis of the disease is poor and the mortality rate is high. Early identification, diagnosis, and multidisciplinary collaborative treatment of CAEBVH are key to improving the prognosis of patients. This article will review the diagnosis and treatment progress of CAEBVH, and provide references for clinicians to diagnose and treat CAEBVH.

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