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1.
International Journal of Traditional Chinese Medicine ; (6): 813-817, 2023.
Article in Chinese | WPRIM | ID: wpr-989718

ABSTRACT

Objective:To evaluate the effect of Tongbu Qijing Acupuncture combined with metformin hydrochloride tablet on polycystic ovary syndrome (PCOS) combined with insulin resistance (IR) of kidney-deficiency phlegm dampness type.Methods:Randomized controlled trial. 84 patients with PCOS and IR in the hospital were enrolled as the observation objects between November 2019 and November 2021. According to random number table method, they were divided into observation group (Tongbu Qijing Acupuncture combined with metformin hydrochloride tablets) and control group (oral metformin hydrochloride tablets), 42 in each group. All were treated for 3 courses of treatment (1 month/course). TCM syndromes were scored before and after treatment. The height, weight, waist circumference and hip circumference of patients were measured to calculate body mass index (BMI) and waist-hip ratio (WHR). The levels of serum TG, TC, LDL-C and HDL-C were detected by biochemical analyzer, fasting blood glucose (FPG) was detected by glucose oxidase method, fasting insulin (FINS) was detected by electrochemiluminescence method, and insulin resistance index (HOMA-IR) was calculated. The recovery rates of menstruation and ovulation were observed and compared after treatment, and the clinical curative effect was evaluated.Results:There were significant differences in total response rate between observation group and control group [95.24% (40/42) vs. 80.95% (34/42); χ2=4.09, P=0.043]. After treatment, scores of TCM syndromes, BMI and WHR in observation group were significantly lower than those in the control group ( t=20.36, 23.77, 3.44, P<0.01). After treatment, serum FPG [(4.86±0.51) nmol/L vs. (5.41±0.55) nmol/L, t=4.75], FINS [(8.31±0.85) mU/L vs. (10.11±1.02) mU/L, t=8.79] levels and HOMA-IR [(1.88±0.19) vs. (2.44±0.25), t=11.97] in observation group were significantly lower than those in the control group ( P<0.01). After treatment, levels of serum TG, TC and LDL-C in observation group were significantly lower than those in the control group ( t=16.54, 4.81, 5.35, P<0.01), while HDL-C was significantly higher than that of the control group ( t=6.78, P<0.01). After treatment, there were significant differences in recovery rates of menstruation and ovulation between observation group and control group [57.14% (24/42), 47.62% (20/42) vs. 80.95% (34/42), 69.05% (29/42); χ2=5.57, 3.97, P<0.05]. Conclusion:Tongbu Qijing Acupuncture combined with metformin hydrochloride tablet can effectively improve syndromes and signs, regulate glucose-lipid metabolism, reduce IR and promote the recovery of menstruation and ovulation in patients with PCOS and IR.

2.
Journal of Peking University(Health Sciences) ; (6): 966-974, 2023.
Article in Chinese | WPRIM | ID: wpr-1010155

ABSTRACT

OBJECTIVE@#To analyze and compare the clinical and laboratory characteristics of macrophage activation syndrome (MAS) in patients with systemic lupus erythematosus (SLE) and adult-onset Still's disease (AOSD), and to evaluate the applicability of the 2016 European League Against Rheumatism/American College of Rheumatology/Paediatric Rheumatology International Trials Organization classification criteria for MAS complicating systemic juvenile idiopathic arthritis (sJIA) in different auto-immune diseases contexts and to propose new diagnostic predictive indicators.@*METHODS@#A retrospective analysis was conducted on the clinical and laboratory data of 24 SLE patients with MAS (SLE-MAS) and 24 AOSD patients with MAS (AOSD-MAS) who were hospitalized at Peking University People's Hospital between 2000 and 2018. Age- and sex-matched SLE (50 patients) and AOSD (50 patients) diagnosed in the same period without MAS episodes were selected as controls. The cutoff values for laboratory indicators predicting SLE-MAS and AOSD-MAS were determined using receiver operating characteristic (ROC) curves. Furthermore, the laboratory diagnostic predictive values for AOSD-MAS were used to improve the classification criteria for systemic juvenile idiopathic arthritis-associated MAS (sJIA-MAS), and the applicability of the revised criteria for AOSD-MAS was explored.@*RESULTS@#Approximately 60% of SLE-MAS and 40% of AOSD-MAS occurred within three months after the diagnosis of the underlying diseases. The most frequent clinical feature was fever. In addition to the indicators mentioned in the diagnosis criteria for hemophagocytic syndrome revised by the International Society for Stem Cell Research, the MAS patients also exhibited significantly elevated levels of aspartate aminotransferase and lactate dehydrogenase, along with a significant decrease in albumin. Hemophagocytosis was observed in only about half of the MAS patients. ROC curve analysis demonstrated that the optimal discriminative values for diagnosing MAS was achieved when SLE patients had ferritin level≥1 010 μg/L and lactate dehydroge-nase levels≥359 U/L, while AOSD patients had fibrinogen levels≤225.5 mg/dL and triglyceride levels≥2.0 mmol/L. Applying the 2016 sJIA-MAS classification criteria to AOSD-MAS yielded a diagnostic sensitivity of 100% and specificity of 62%. By replacing the less specific markers ferritin and fibrinogen in the 2016 sJIA-MAS classification criteria with new cutoff values, the revised criteria for classifying AOSD-MAS had a notable increased specificity of 86%.@*CONCLUSION@#Secondary MAS commonly occurs in the early stages following the diagnosis of SLE and AOSD. There are notable variations in laboratory indicators among different underlying diseases, which may lead to misdiagnosis or missed diagnosis when using uniform classification criteria for MAS. The 2016 sJIA-MAS classification criteria exhibit high sensitivity but low specificity in diagnosing AOSD-MAS. Modification of the criteria can enhance its specificity.


Subject(s)
Adult , Humans , Child , Macrophage Activation Syndrome/complications , Arthritis, Juvenile/diagnosis , Still's Disease, Adult-Onset/diagnosis , Retrospective Studies , Lupus Erythematosus, Systemic/diagnosis , Fibrinogen , Ferritins
3.
Chinese Journal of Pathology ; (12): 3-6, 2022.
Article in Chinese | WPRIM | ID: wpr-935461

ABSTRACT

With the technological progresses and applications of human genome sequencing, bioinformatics analysis and data mining, and molecular pathology and artificial intelligence-assisted pathological diagnosis, the development of clinical medicine is moving towards the era of precision diagnosis and treatment. In the context of this era, the traditional diagnostic pathology is facing unprecedented opportunities and challenges in our history and is striving towards the "next-generation diagnostic pathology" (NGDP). NGDP is based on histomorphology and clinical data, and characterized by the combination of molecular detection and bioinformatics analysis, intelligent sampling and process quality control, intelligent diagnosis and remote consultation, lesion visualization and "non-invasive" pathology as well as other innovative cutting edge interdisciplinary technologies. The NGDP reports will include the results from multi-omics and cross-scale integrated diagnosis for final diagnosis. NGDP will also be applied for predicting disease progression and outcomes, and determining optional therapeutics as well as assessing treatment responses, so that a novel "golden standard" of disease diagnosis can be established. In the near fature, it is necessary to stimulate the innovative vitality of pathology disciplines, accelerate the maturity and application for NGDP, update the theory and technical system of pathology, and perform its important applicable role in the prevention, diagnosis, treatment of diseases so that the futher development of clinical medicine will be promoted and the strategy for maintenance of being healthy in China will be served.


Subject(s)
Humans , Artificial Intelligence , China , Computational Biology , Pathology, Molecular
4.
Chinese Journal of Epidemiology ; (12): 418-423, 2022.
Article in Chinese | WPRIM | ID: wpr-935405

ABSTRACT

Real-world data study evidence, as an important part of evaluating the safety and effectiveness of drugs and devices, has attracted increasing attention from regulatory agencies and scholars both at home and abroad, and has become an essential source of evidence to support the development and review of drugs and devices. This paper systematically discusses the process and mode of real-world data system construction based on the preliminary practical study of real-world data according to the guidelines/technical specifications issued by regulatory agencies and academic research results. This study result provides not only reference for the generation of clinical evaluation evidence to meet the regulatory requirements for innovative drugs and devices, but also reference for researchers, sponsors and regulators to carry out real-world data studies successfully.

5.
Cancer Research and Clinic ; (6): 180-183, 2022.
Article in Chinese | WPRIM | ID: wpr-934653

ABSTRACT

Objective:To investigate the screening values of immunocytochemical P16/Ki-67 double staining, P16 INK4α single staining and high-risk human papillomavirus (HR-HPV) testing for high-grade cervical lesions. Methods:The clinical data of 622 patients who underwent cervical thin-layer liquid-based cytology (TCT) and HR-HPV testing in General Hospital of Taiyuan Iron and Steel (Group) Co., Ltd. from March 2019 to July 2021 were retrospectively analyzed. The remaining cytological specimens were detected by P16/Ki-67 double staining and P16 INK4α single staining. Among them, 334 patients with TCT results suggesting atypical squamous cell of undetermined significance (ASCUS) and above and HPV-positive underwent colposcopy pathological biopsy. Using pathological results as reference, the positive predictive value, sensitivity, specificity and accuracy of P16/Ki-67 double staining, P16 INK4α single staining and HR-HPV testing for screening of high-grade squamous intraepithelial neoplasia (HSIL) and cervical cancer were compared. Results:Taking the results of histopathology as references, combined with the results of TCT, 31 of 622 patients were HSIL, of which 22 (71.0%) were positive for P16/Ki-67 double staining, 23 (74.2%) were positive for P16 INK4α single staining, and 25 (80.6%) were positive for HR-HPV testing; 4 cases were cervical cancer, and the positive rates of the three detection methods were all 100.0% (4/4). Among 622 patients, the positive rates of P16/Ki-67 double staining, P16 INK4α single staining and HR-HPV testing for screening of HSIL and cervical cancer were 13.99% (87/622), 25.40% (158/622) and 21.38% (133/622); the positive predictive values were 29.89%, 17.09% and 21.08%; the accuracies were 91.19%, 78.94% and 83.28%; the specificities were 89.77%, 77.98% and 82.46%; the sensitivities were 74.29%, 77.14% and 82.86%. The positive rate, positive predictive value, specificity and accuracy of P16/Ki-67 double staining were higher than those of P16 INK4α single staining and HR-HPV testing, and the differences were statistically significant ( z values were -5.062 and -3.418, 2.328 and 2.450, 5.436 and 3.570, 6.043 and 4.161, all P < 0.05); the sensitivity of HR-HPV testing was higher than that of P16/Ki-67 double staining and P16 INK4α single staining, but the differences were not statistically significant ( z values were -0.890 and 1.017, both P > 0.05). Conclusions:HR-HPV testing is more suitable for primary cervical lesion screening; P16/Ki-67 double staining can be used as a potential combined cell screening tool or an effective triage tool; P16 INK4α single staining has certain limitations.

6.
Journal of Peking University(Health Sciences) ; (6): 278-282, 2022.
Article in Chinese | WPRIM | ID: wpr-936147

ABSTRACT

OBJECTIVE@#To evaluate the sensitivity and specificity of Pollard' s classification criteria(2010) for the diagnosis of rheumatoid arthritis (RA) patients withfibromyalgia (FM) in Chinese patients, and to assess the clinical features and psychological status of RA-FM patients in a real-world observational setting.@*METHODS@#Two hundred and two patients with rheumatoid arthritis were enrolled from the outpatients in Rheumatology and Immunology Department in Peking University People' s Hospital. All the patients were evaluated whether incorporating fibromyalgia translation occured using the 1990 American College of Rheumatolgy (ACR)-FM classification criteria. Forty two RA patients were concomitant with FM, while the other one hundred and sixty RA patients without FM were set as the control group.@*RESULTS@#There was no significant difference in general demography between the two groups (P>0.05). In this study, the Pollard' s classification criteria (2010) for RA-FM in Chinese patients had a high sensitivity of 95.2% and relatively low specificity of 52.6%. Compared with those patients without FM, RA patients with FM (RA-FM patients) had higher Disease Activity Scale in 28 joints (DAS-28) score (5.95 vs. 4.38, P=0.011) and much more 28-tender joint counts (TJC) (16.5 vs.4.5, P < 0.001).RA-FM patients had worse Health Assessment Questionnaire (HAQ) score (1.24 vs. 0.66, P < 0.001) and lower SF-36 (28.63 vs. 58.22, P < 0.001). Fatigue was more common in RA-FM patients (88. 1% vs. 50.6%, P < 0.001) and the degree of fatigue was significantly increased in RA-FM patients (fatigue VAS 5.55 vs. 3.55, P < 0.001). RA-FM patients also had higher anxiety (10 vs.4, P < 0.001) and depression scores (12 vs.6, P < 0.001). erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), morning stiffness time and 28-swollen joint counts (SJC) showed no difference between these two groups.@*CONCLUSION@#The Pollard' s classification criteria (2010) for RA-FM are feasible in Chinese rheumatoid arthritis patients. The Pollard' s classification criteria is highly sensitive in clinical application, while the relativelylow specificity indicates that various factors need to be considered in combination. RA patients with FM result in higher disease activity, worse function aland psychological status. RA patients with FM also have poorer quality of life. DAS-28 scores may be overestimated in RA patients with FM. In a RA patient thatdoes not reach remission, the possibility of fibromyalgia should be con-sidered.


Subject(s)
Humans , Arthritis, Rheumatoid/diagnosis , Fatigue/etiology , Fibromyalgia/diagnosis , Quality of Life , Severity of Illness Index
7.
China Journal of Chinese Materia Medica ; (24): 2015-2020, 2022.
Article in Chinese | WPRIM | ID: wpr-928140

ABSTRACT

Essential oils(EOs) from Chinese medicinals, which can be used as adjuvants and exert certain therapeutic effect, are directly used in Chinese medicine formulas. Conventional research strategy for EOs from Chinese medicinals is to compare the efficacy of the prescriptions before and after the addition of EOs, and the penetration-enhancing mechanisms of EOs remain unclear. In modern research on EOs from Chinese medicinals, the method for studying chemical penetration enhancers is often used, which fails to reflect the overall efficacy of EOs. This study clarified the property regularity of EOs from Chinese medicinals as transdermal penetration enhancers, and thereby proposed a research model which integrated the medicinal and adjuvant properties of EOs from Chinese medicinals via "component-delivery-effect" characterization route. The core concept is that constituents of EOs from Chinese medicinals and their delivery process play a key role in their external application. This research model is expected to serve as a reference for further research on EOs from Chinese medicinals for transdermal application.


Subject(s)
Adjuvants, Pharmaceutic , Administration, Cutaneous , China , Drugs, Chinese Herbal/pharmacology , Oils, Volatile/pharmacology
8.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 80-87, 2022.
Article in Chinese | WPRIM | ID: wpr-940355

ABSTRACT

ObjectiveTo study the in vitro anti-hepatocarcinoma HepG2 cell mechanism of Jaranol. MethodThe methyl thiazolyl tetrazolium (MTT) assay was employed to examine the inhibition of Jaranol (0, 5, 10, 25, 50, 100, 150, 300 μmol·L-1) on HepG2 cell proliferation at different time (24 , 48 , 72 h), annexin V-fluorescein isothiocyante/propidium iodide (Annexin V-FITC/PI) kit to detect the effect of Jaranol (0, 3, 15, 75 μmol·L-1) on HepG2 cell apoptosis, and Western blot to determine the influence of Jaranol on the expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) in HepG2 cells. Transcriptome sequencing was performed to analyze the differential expression of genes and changes of related signaling pathways after the treatment of HepG2 cells with Jaranol (15 μmol·L-1). Real-time PCR was carried out to verify the relative mRNA content of differential genes [TEK, platelet-derived growth factor receptor α (PDGFRA), spleen tyrosine kinase (SYK), phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma (PIK3CG), Janus kinase 3 (JAK3), membrane-associated guanylate kinase inverted 2 (MAGI2)]. ResultCompared with the blank group, Jaranol decreased HepG2 proliferation (P<0.05, P<0.01), increased apoptosis rate of HepG2 cells (P<0.05, P<0.01), raised Bax expression (P<0.05, P<0.01), and reduced Bcl-2 expression (P<0.05, P<0.01). Transcriptome sequencing yielded 59 000 regulated genes, 125 of which showed significantly different expression, with 47 up-regulated and 74 down-regulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the differential genes related to apoptosis in the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway changed significantly after drug addition. The mRNA expression of TEK, PDGFRA, SYK, PIK3CG, JAK3, and MAGI2 decreased in Jaranol (15 μmol·L-1) group compared with that in the control group (P<0.05). ConclusionIn vitro cytological experiment verified that Jaranol inhibited the proliferation of HepG2 cells and promoted the apoptosis, possibly by influencing the expression of some differential genes in the PI3K/Akt signaling pathway. The result lays an experimental basis for the follow-up study of the anti-tumor effect of Jaranol, and the further development and utilization of flavonoids.

9.
Journal of Peking University(Health Sciences) ; (6): 1067-1071, 2021.
Article in Chinese | WPRIM | ID: wpr-942298

ABSTRACT

OBJECTIVE@#To explore the abnormal manifestations and clinical features of patients with gout according to the location of crystal deposits: in articulars or in tendons.@*METHODS@#A total of 105 patients with gout who were continuously treated in the Department of Rheumatology and Immunology of Peking University People's Hospital from June 2019 to December 2019 were selected and their knees, ankles, toes and painful joints and tendons were examined by high-frequency ultrasound. Then we grouped them according to the presence or absence of sodium urate crystals and the location of the crystals, collected their clinical data, and analyzed the clinical characteristics.@*RESULTS@#Among the 105 patients, 25 patients had no crystal deposits in the joints or tendons (as the non-crystal group), 43 patients had intra-articular crystals (as the joint group), and 37 patients had intra-tendon crystals with or without intra-articular crystals (as the tendon group). Among them, the most involved part of sodium urate crystals deposited in the joints was the metatarsophalangeal joint (29 cases, 67.4%), followed by knee joints (10 cases, 23.2%), ankle joints (9 cases, 20.9%). The most involved part of sodium urate crystals deposited in the tendon was the quadriceps tendon (16 cases, 43.2%), followed by the Achilles tendon (13 cases, 35.1%), the patellar tendon (12 cases, 32.4%), and the three heads of brachii tendons (5 cases, 13.5%). The three groups were compared using multi-sample analysis of variance/multi-sample rank sum test. Age, age of first increase in uric acid (UA), serum glucose (Glu) level and C reactive protein (CRP) were all significantly different. After multiple comparisons, compared with the non-crystal group, age, the age of first increase in uric acid, and CRP were significantly higher in the tendon group. There was no significant difference between the non-crystal group and the joint group. There was no significant difference between the tendon group and the joint group.@*CONCLUSION@#In patients with gout, it is common for ultrasound to find crystals deposited in joints or tendons. The most commonly affected parts include the metatarsophalangeal joint, knee joint, ankle joint, quadriceps tendon, Achilles tendon, patellar tendon, and triceps tendon. There were significant differences among the three groups in age, age of first increase in uric acid, CRP and blood glucose, and the proportion of urinary calculi in patients with crystal deposits was significantly higher than those without crystal deposits.


Subject(s)
Humans , Achilles Tendon , Gout , Knee Joint , Metatarsophalangeal Joint/diagnostic imaging , Uric Acid
10.
China Journal of Chinese Materia Medica ; (24): 312-319, 2021.
Article in Chinese | WPRIM | ID: wpr-878976

ABSTRACT

Breast tumor has become one of the malignant tumors with the highest incidence, and is a serious threat to human health, especially to women. Chemotherapy is an important anti-breast tumor therapy, which can be used in almost every stage of breast tumor therapy alone or in the combination with surgery and radiation therapy. Alkaloids are a kind of ubiquitous natural products, and important active components of various medicinal plants. A large number of studies have shown that alkaloids could exert an anti-breast tumor effect by inhibiting proliferation, metastasis and angiogenesis, resisting mitosis, promoting apoptosis and autophagy, and triggering cell cycle arrest. The extensive anti-breast tumor effect makes alkaloids an important candidate drug source. This paper reviews the anti-breast tumor mechanism of natural products of alkaloids.


Subject(s)
Female , Humans , Alkaloids/pharmacology , Apoptosis , Autophagy , Breast Neoplasms/drug therapy , Cell Cycle Checkpoints , Cell Line, Tumor , Cell Proliferation
11.
China Journal of Chinese Materia Medica ; (24): 3410-3421, 2021.
Article in Chinese | WPRIM | ID: wpr-887992

ABSTRACT

This study aims to investigate metabolic activities of psoralidin in human liver microsomes( HLM) and intestinal microsomes( HIM),and to identify cytochrome P450 enzymes( CYPs) and UDP-glucuronosyl transferases( UGTs) involved in psoralidin metabolism as well as species differences in the in vitro metabolism of psoralen. First,after incubation serial of psoralidin solutions with nicotinamide adenine dinucleotide phosphate( NADPH) or uridine 5'-diphosphate-glucuronic acid( UDPGA)-supplemented HLM or HIM,two oxidic products( M1 and M2) and two conjugated glucuronides( G1 and G2) were produced in HLM-mediated incubation system,while only M1 and G1 were detected in HIM-supplemented system. The CLintfor M1 in HLM and HIM were 104. 3,and57. 6 μL·min~(-1)·mg~(-1),respectively,while those for G1 were 543. 3,and 75. 9 μL·min~(-1)·mg~(-1),respectively. Furthermore,reaction phenotyping was performed to identify the main contributors to psoralidin metabolism after incubation of psoralidin with NADPH-supplemented twelve CYP isozymes( or UDPGA-supplemented twelve UGT enzymes),respectively. The results showed that CYP1 A1( 39. 5 μL·min~(-1)·mg~(-1)),CYP2 C8( 88. 0 μL·min~(-1)·mg~(-1)),CYP2 C19( 166. 7 μL·min~(-1)·mg~(-1)),and CYP2 D6( 9. 1 μL·min~(-1)·mg~(-1)) were identified as the main CYP isoforms for M1,whereas CYP2 C19( 42. 0 μL·min~(-1)·mg~(-1)) participated more in producing M2. In addition,UGT1 A1( 1 184. 4 μL·min~(-1)·mg~(-1)),UGT1 A7( 922. 8 μL·min~(-1)·mg~(-1)),UGT1 A8( 133. 0 μL·min~(-1)·mg~(-1)),UGT1 A9( 348. 6 μL·min~(-1)·mg~(-1)) and UGT2 B7( 118. 7 μL·min~(-1)·mg~(-1)) played important roles in the generation of G1,while UGT1 A9( 111. 3 μL·min~(-1)·mg~(-1)) was regarded as the key UGT isozyme for G2. Moreover,different concentrations of psoralidin were incubated with monkey liver microsomes( MkLM),rat liver microsomes( RLM),mice liver microsomes( MLM),dog liver microsomes( DLM) and mini-pig liver microsomes( MpLM),respectively. The obtained CLintwere used to evaluate the species differences.Phase Ⅰ metabolism and glucuronidation of psoralidinby liver microsomes showed significant species differences. In general,psoralidin underwent efficient hepatic and intestinal metabolisms. CYP1 A1,CYP2 C8,CYP2 C19,CYP2 D6 and UGT1 A1,UGT1 A7,UGT1 A8,UGT1 A9,UGT2 B7 were identified as the main contributors responsible for phase Ⅰ metabolism and glucuronidation,respectively. Rat and mini-pig were considered as the appropriate model animals to investigate phase Ⅰ metabolism and glucuronidation,respectively.


Subject(s)
Animals , Dogs , Mice , Rats , Benzofurans , Coumarins , Glucuronides , Glucuronosyltransferase/metabolism , Kinetics , Microsomes, Liver/metabolism , Phenotype , Species Specificity , Swine , Swine, Miniature/metabolism
12.
West China Journal of Stomatology ; (6): 434-440, 2021.
Article in English | WPRIM | ID: wpr-887755

ABSTRACT

OBJECTIVES@#To investigate the expression of Ki-67, Cyclin D1, P53, and P16 in patients with oral leukoplakia (OLK) and OLK cancerization who have aspicy diet in Chengdu.@*METHODS@#Thirtypatients with OLK andspicy diet and 15 patients with OLK without spicy diet in Chengdu were divided into three groups: hyperplastic OLK (OLK-), OLK with mild to moderate dysplasia (OLK+), and severe dysplastic  OLK or oral squamous cell carcinoma (OSCC) transforming from OLK (OLK++/OSCC). The expression of Ki-67, Cyclin D1, P53, and P16 were detected by immunohistochemistry and statistically analyzed.@*RESULTS@#The expression of Ki-67 and P53 in patients with or without spicy diet in the OLK+and OLK++/OSCC groups were stronger than that of the OLK- group (@*CONCLUSIONS@#Spicy diet did not have an influence on the expression of Ki-67, Cyclin D1, P53, and P16 in patients with OLK and OSCC. The expression of Ki-67, Cyclin D1, and P53 increased with the development of OLK, whereas P16 showed opposite expression trend.


Subject(s)
Humans , Carcinoma, Squamous Cell , Cyclin D1 , Diet , Head and Neck Neoplasms , Ki-67 Antigen , Leukoplakia, Oral , Mouth Neoplasms , Tumor Suppressor Protein p53
13.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 98-105, 2021.
Article in Chinese | WPRIM | ID: wpr-906428

ABSTRACT

Objective:To observe the effects of modified Huangqi Biejiatang combined with auricular acupressure on diabetic peripheral neuropathy (DPN) due to Qi and Yin deficiency and serum myeloid differentiation factor 88/inhibitor of nuclear factor-<italic>κ</italic>B (MyD88/I<italic>κ</italic>B) signaling pathway. Method:One hundred and forty cases were randomly divided into an observation group (<italic>n</italic>=70) and a control group (<italic>n</italic>=70). In addition to routine treatments like dietary intervention and the regulation of fasting blood glucose (FBG) and blood pressure, the modified Huangqi Biejiatang combined with auricular acupressure was further provided in the observation group, while mecobalamine was administered in the control group. After four-week intervention, the toronto clinical scoring system (TCSS) score, traditional Chinese medicine (TCM) syndrome score, the conduction velocities of motor and sensory nerves (median nerve, common peroneal nerve, tibial nerve, and ulnar nerve), glucose metabolism indexes [fasting plasma glucose (FPG), 2 h postprandial blood glucose (2 h PG), and hemoglobin A1c (HbA1c)], intestinal genera (<italic>Clostridium</italic>, <italic>Prauserella</italic>, <italic>Bacteroides</italic>, and <italic>Faecalibacterium</italic>), as well as the serum MyD88, I<italic>κ</italic>B<italic>α</italic>, and phosphorylated I<italic>κ</italic>B<italic>α </italic>(p-I<italic>κ</italic>B<italic>α</italic>) levels in the MyD88/I<italic>κ</italic>B signaling pathway before and after treatment were observed in the two groups, for comparing their clinical efficacy and safety. Result:The total effective rate of the observation group was 85.3% (58/68), which was higher than 48.5% (32/66) of the control group (<italic>χ</italic><sup>2</sup>=6.143, <italic>P</italic><0.05). The comparison with the control group revealed that the scores of TCSS and TCM syndrome, the levels of FPG, 2 h PG, HbA1c, MyD88, and p-I<italic>κ</italic>B<italic>α</italic>, as well as the abundances of <italic>Clostridium</italic> and <italic>Prauserella</italic> in the observation group were decreased (<italic>P</italic><0.05), while the conduction velocities of motor and sensory nerves (median nerve, common peroneal nerve, tibial nerve, and ulnar nerve) were significantly accelerated (<italic>P</italic><0.05). Besides, the abundances of <italic>Bacteroides</italic> and <italic>Faecalibacterium</italic> and I<italic>κ</italic>B<italic>α</italic> level were significantly elevated (<italic>P</italic><0.05). The incidence of adverse reactions in the observation group was 1.5% (1/68), lower than 12.1% (8/66) in the control group (<italic>χ</italic><sup>2</sup>=4.328, <italic>P</italic><0.05). Conclusion:The modified Huangqi Biejiatang combined with auricular acupressure alleviates DPN due to Qi and Yin deficiency, which may be attributed to the regulation of serum MyD88/I<italic>κ</italic>B signaling pathway.

14.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 80-87, 2021.
Article in Chinese | WPRIM | ID: wpr-906022

ABSTRACT

Objective:To observe the therapeutic effect of modified Fuyuanwan combined with auricular acupressure bean on stage Ⅱ, Ⅲ diabetic nephropathy and its effect on serum janus kinase (JAK)/ signal transducer and activator of tranions (STAT) signaling pathway. Method:A total of 180 cases were randomly divided into control group and observation group, 90 cases in each group. Losartan potassium, modified Fuyuanwan combined with auricular acupressure bean were given respectively for 12 weeks. Renal function indexes [blood urea nitrogen (BUN), serum creatinine (SCr), urinary albumin excretion rate (UAER), 24 h urinary protein quantitative (24 h Upor)], relative abundance of intestinal flora (verruca microflora, scleriobacteriae, deferribacter, proteobacteria), oxidative stress indicators [advanced oxidation protein products (AOPPs), reactive oxygen species (ROS), glutathione peroxidase (GSH-Px), total superoxide dismutase (TSOD)], renal blood flow index [end-diastolic blood flow velocity (EDV), peak systolic value (PSV), pulse index (PI), blood flow resistance index (RI)], JAK/STAT signaling pathway [JAK, phosphorylated JAK (p-JAK), STAT, phosphorylated STAT (p-STAT) were observed before and after treatment. The safety indexes of two groups were evaluated after treatment. The efficacy was observed after treatment and followed up for 1 years and 2 years. Result:After treatment and follow-up for 1, 2 years, the total effective rates of patients in observation group were 97.8% (87/89), 81.6% (71/87), 59.8% (49/82), respectively, observation group which were significantly higher than those in control group of 79.3%(69/87),57.8%(48/83),37.2%(29/78) (<italic>χ</italic><sup>2</sup>=4.016, <italic>χ</italic><sup>2</sup>=4.503, <italic>χ</italic><sup>2</sup>=4.769, <italic>P</italic><0.05). Compared with control group after treatment, UAER, BUN, SCr, 24 h Upor, firmicutes, actinobacillus, proteobacteria, AOPPs, ROS, PI, RI, p-JAK, p-STAT3 in observation group were significantly decreased (<italic>P</italic><0.05,<italic>P</italic><0.01), microflora verruca, GSH-PX, TSOD, JAK, STAT3 were significantly increased (<italic>P</italic><0.05,<italic>P</italic><0.01), EDV and PSV were significantly accelerated (<italic>P</italic><0.05,<italic>P</italic><0.01). The incidence of adverse reactions was 1.1% (1/89) in observation group, lower than 13.8% (12/87) in control group (<italic>χ</italic><sup>2</sup>=5.127, <italic>P</italic><0.05). Conclusion:Modified Fuyuanwan combined with auricular acupressure bean can significantly improve the curative effect of stage Ⅱ, Ⅲ diabetic nephropathy, and its mechanism of action may be related to the serum JAK/STAT signaling pathway.

15.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 112-117, 2021.
Article in Chinese | WPRIM | ID: wpr-905995

ABSTRACT

Objective:To observe the clinical efficacy of modified Huqianwan combined with auricular acupressure with seeds on girls with central precocious puberty (CPP) due to Yin deficiency and fire hyperactivity. Method:A total of 150 cases were randomly divided into three groups (A group,B group, and C group),with 50 cases in each group. The cases received modified Huqianwan,Zhibo Dihuangwan,and modified Huqianwan combined with auricular acupressure with seeds, respectively, for 12 weeks. The traditional Chinese medicine (TCM) symptoms,bone age difference/chronological age difference (ΔBA/ΔCA), predicted adult height (PAH),ovarian volume,uterine volume,breast nodule index,serum sex hormones [follicle-stimulating hormone (FSH),luteinizing hormone (LH),and estradiol (E<sub>2</sub>)],and serum kisspeptin-1 (KISS-1)/G protein-coupled receptor 54 (GPR54)/hypothalamic gonadotropin-releasing hormone (GnRH) signaling pathway of the three groups were observed before and after treatment. The clinical efficacy and safety of the three groups were evaluated. Result:The total effective rate was 97.9% in the C group, which was higher than 81.3% in the A group (<italic>χ</italic><sup>2</sup>=4.516,<italic>P</italic><0.05) and 71.4% in the B group (<italic>χ</italic><sup>2</sup>=5.162,<italic>P</italic><0.05). Compared with the results in the A group after treatment,TCM syndrome,ΔBA/ΔCA,E<sub>2</sub>,LH,KISS-1,GPR54,and GnRH decreased(<italic>P</italic><0.05),and ovarian volume,uterine volume,and breast nodule index were reduced (<italic>P</italic><0.05),but PAH increased in the C group (<italic>P</italic><0.05). Compared with the B group after treatment,the C group showed decreased TCM syndrome,ΔBA/ΔCA, FSH,E<sub>2</sub>,LH,KISS-1,GPR54,and GnRH (<italic>P</italic><0.05),reduced ovarian volume,uterine volume,and breast nodule index (<italic>P</italic><0.05),and increased PAH(<italic>P</italic><0.05). During the study,there was one case of anorexia in the A group,and no obvious adverse reactions were found in the B group and the C group. Conclusion:Modified Huqianwan combined with auricular acupressure with seeds can significantly increase the PAH of girls with CPP due to Yin deficiency and fire hyperactivity and improve the serum sex hormones and the KISS-1/GPR54/GnRH signaling pathway.

16.
Chinese Traditional and Herbal Drugs ; (24): 5929-5933, 2020.
Article in Chinese | WPRIM | ID: wpr-846009

ABSTRACT

Objective: To study the chemical constituents from Stelleropsis tianschanica. Methods: The constituents were isolated from S. tianschanica and purified by column chromatography, and the structures were identified by spectral analysis and chemical methods. Results: A total of 12 compounds were isolated from S. tianschanica, including seven flavonoids compounds, two lignans compounds, and the other three compounds, and the structures were identified as wikstrol A (1), wikstrol B (2), viburnolide A (3), genkwanol B (4), genkwanol C (5), stelleranol (6), dipropyl phthalate (7), diisobutyl phthalate (8), 1,3,5-triol-2-methoxybenzene (9), coniferin (10), syringin (11), and acacetin (12). Conclusion: Compounds 1-2, 5-6, 9-12 are isolated from the genus Stelleropsis for the first time.

17.
Acta Pharmaceutica Sinica ; (12): 241-246, 2020.
Article in Chinese | WPRIM | ID: wpr-789028

ABSTRACT

To detect the inhibitory effect of Astragalus protein on the proliferation of hepatocellular carcinoma cell line HepG2, transcriptomics was used to explore the anti-tumor mechanism of Astragalus protein. The dried roots of Astragalus was precipitated by ammonium sulfate to obtain Huang Qi protein (HQP) with different molecular weights. The effect of HQP on HepG2 and its toxic effect were detected by hemocytometry. Cell necrosis was detected by flow cytometry and Hoechst/propidium iodide (PI) double staining. The necrotic marker protein receptor interacting serine/threonine kinase 1 (RIP1) was determined by Western blot. Transcriptome sequencing was performed on the control group and dosing group RNA, and differential expression genes were analyzed for RNA-seq results. qRT-PCR was used to verified the relative mRNA expression levels of candidate genes. The results showed that the inhibition of HepG2 proliferation was more obvious with the increase of HQP concentration. When the concentration of HQP was 100 μg·mL-1, the necrosis rate increased to 18.78%, and the number of red necrotic cells stained with PI was observed under the microscope. The Western blot results showed an increase in RIP1 protein levels. The results of RNA-seq analysis showed that 26 000 related genes were regulated by HQP, and 979 genes were more regulated. KEGG analysis found that some differentially expressed genes were associated with p53 signaling pathway, and qRT-PCR further verified that the sequencing results were reliable. HQP may cause programmed necrosis of HepG2 cells and may be involved in the p53 signaling pathway.

18.
Chinese Journal of Medical Genetics ; (6): 603-608, 2020.
Article in Chinese | WPRIM | ID: wpr-826525

ABSTRACT

OBJECTIVE@#To explore the cause for the failure of non-invasive prenatal testing (NIPT) and feasibility of repeated testing.@*METHODS@#Clinical data, test results and pregnancy outcomes of 40 311 pregnant women who received NIPT test from January 2011 to December 2018 were reviewed.@*RESULTS@#Among all the pregnant women, 1116 cases failed in the first test, 9 cases (0.81%) had fetal free DNA concentration lower than 4%, 663 cases (59.41%) were retested after the establishment of Z value gray area, and the remainder 444 cases (39.78%) needed to be retested after the blood collection due to the fetal free DNA concentration lower than 4%. After retesting, 1069 cases (95.78%) obtained effective NIPT results. The results showed that 53 cases were at high risk (6 cases for trisomy 21, 6 cases for trisomy 18, 13 cases for trisomy 13, 16 cases for sex chromosomal abnormality, 12 cases for chromosomal copy number variation). Forty-eight cases were selected for invasive prenatal diagnosis, and 2 cases of 47, XXY and 2 CNV were confirmed. A total of 47 cases (0.12%) did not obtain results because the concentration of fetal free DNA was lower than 4%. Only 16 cases (34%) chose invasive prenatal diagnosis.@*CONCLUSION@#Repeated detection of the gray area of Z value can reduce the false positive rate of NIPT and invasive prenatal diagnosis, and the feasibility of repeated detection is high. In the case of fetal free DNA concentration lower than 4%, the success rate of obtaining effective NIPT results by re-sampling and re-detection increases with the increase of gestational age, but may delay the diagnosis for fetal aneuploidies. Therefore, personalized estimation should be made according to gestational age and clinical indications. It is suggested that pregnant women should choose invasive prenatal diagnosis when they have failed in the retest.

19.
West China Journal of Stomatology ; (6): 297-300, 2020.
Article in Chinese | WPRIM | ID: wpr-827542

ABSTRACT

OBJECTIVE@#To explore the treatment conditions of acid decalcified specimens and improve the poor quality of sections and unclear structure of hematoxylin-eosin (HE) staining caused by the change in pH in tooth and hard tissue after acid decalcification.@*METHODS@#A total of 20 cases of oral pathological specimens that contain hard tissues were decalcified and treated with routine treatment, concentrated ammonia water immersion treatment, and saturated lithium carbonate solution immersion treatment. The quality and HE staining effects of hard tissue sections treated with different methods were compared.@*RESULTS@#Compared with routine treatment, lithium carbonate saturated solution treatment showed complete sections. Hematoxylin is strongly stained, the nucleus is clear, and the cytoplasm is bright.@*CONCLUSIONS@#Soaking acid decalcified specimens in lithium carbonate saturated solution before embedding in dehydration can neutralize the acidic environment of the tissue. The quality of sections and HE staining effect are improved and are suitable for the pretreatment of acid decalcified tissue samples of oral pathology.


Subject(s)
Eosine Yellowish-(YS) , Hematoxylin , Staining and Labeling , Tooth
20.
Journal of Peking University(Health Sciences) ; (6): 1040-1047, 2020.
Article in Chinese | WPRIM | ID: wpr-942114

ABSTRACT

OBJECTIVE@#To investigate the clinical characteristics of patients with elderly-onset rheumatoid arthritis (EORA), and the risk factors of EORA complicated with cardiovascular disease (CVD).@*METHODS@#A cross-sectional study was conducted in Peking University People's Hospital from July 2009 to December 2014 and 1 116 patients were recruited. The patients' characteristics and CVD, including ischemic heart disease, cerebral and peripheral vascular disease, were recorded. The patients were divided into EORA group (n=212) and younger-onset rheumatoid arthritis (YORA) group (n=904) according to the age of onset ≥60 years and < 60 years. Then, the differences between the groups were analyzed by Student's t test, Mann-Whitney U test or χ2test, and risk influencing CVD were analyzed using Logistic regression.@*RESULTS@#There was no significant difference in the disease activity between the EORA and YORA groups. The proportion of male, pulmonary interstitial disease (ILD), and numbers of deformity joint count (DJC) were significantly higher in the EORA group compared with the YORA group [32.1% vs. 18.5%, χ2=19.11, P < 0.001; 23.6% vs. 13.6%, χ2=16.50, P < 0.001; 6 (2, 12) vs. 3 (2, 7), Z=-3.60, P < 0.001], while the prevalence of Sjögren's syndrome was lower than that of the YORA group (13.5% vs. 5.2%, χ2=11.29, P=0.001). Moreover, there were lower prevalences in the patients treated with disease-modifying antirheumatic drugs (DMARDs) in EORA group (35.4%) than in YORA group (26.7%) (χ2=6.43, P=0.011), especially in methotrexate (MTX), hydroxychloroquine (HCQ) and sulfasalazine (SSZ). In addition, the patients with EORA had a higher prevalence of CVD (27.8%) than the YORA group (11.6%, χ2=40.46, P < 0.001), accompanied with higher prevalence of smoking, hypertension, and hyperlipidemia. Multivariate Logistic regression analysis showed that elder age (OR=1.10, 95%CI: 1.00-1.20), DJC (OR=3.17, 95%CI: 1.04-9.68), rheumatoid nodules (OR=3.56, 95%CI: 1.03-12.23), hypertension (OR=2.37, 95%CI: 1.09-5.13) and hyperlipidemia (OR=8.85, 95%CI: 2.50-31.27) were independent risk factors, while HCQ (OR=0.22, 95%CI: 0.07-0.70) and MTX (OR=0.32, 95%CI: 0.14-0.73) were protective factors of EORA complicated with CVD.@*CONCLUSION@#Compared with YORA, patients with EORA have higher ratio of male, ILD and DJC, which may be attributed to inappropriate therapies. EORA is more likely to be complicated with CVD than YORA. Elder age, DJC, rheumatoid nodules, hypertension, and hyperlipidemia are independent risk factors, while HCQ and MTX are protective factors of EORA complicated with CVD.


Subject(s)
Aged , Humans , Male , Age of Onset , Antirheumatic Agents/therapeutic use , Arthritis, Rheumatoid/epidemiology , Cardiovascular Diseases/etiology , Cross-Sectional Studies , Risk Factors
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