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1.
Journal of Experimental Hematology ; (6): 1136-1140, 2021.
Article in Chinese | WPRIM | ID: wpr-888529

ABSTRACT

OBJECTIVE@#To evaluate the efficacy and safety of CHOP regimen based on doxorubicin hydrochloride liposome in the initial treatment of elderly patients with diffuse large B-cell lymphoma (DLBCL).@*METHODS@#Thirty-one patients with DLBCL treated from January 1, 2012 to December 31, 2019 were analyzed retrospectively, their median age was 83 (71-95) years old, and all of them were in Ⅲ-Ⅳ stage, including 17 cases who had international prognostic index (IPI) ≥ 3. The patients were treated with R-CHOP and CHOP regimens based on doxorubicin hydrochloride liposome. The efficacy and safety were evaluated during and after treatment.@*RESULTS@#A total of 219 chemotherapy cycles and 7 median cycles were performed in 31 patients. The overall response (OR) rate and complete remission (CR) rate was 80.7% (25/31) and 61.3% (19/31), respectively, as well as 2 cases (6.5%) stable, 4 cases (12.9%) progressive. The main toxicities were as follows: the incidence of grade Ⅲ -Ⅳ neutropenia was 29% (9/31); two patients (6.5%) developed degree Ⅰ-Ⅱ cardiac events, which were characterized by new degree Ⅰ atrioventricular block; there were no cardiac events requiring emergency treatment and discontinuation of chemotherapy. The 1-year, 2-year and 3-year overall survival rate was 83.9%, 77.4% and 61.3%, respectively. The 1-year, 2-year and 3-year progression-free survival rate was 77.4%, 64.5% and 61.3%, respectively.@*CONCLUSION@#The chemotherapy regimen based on doxorubicin hydrochloride liposome has better efficacy and higher cardiac safety for elderly patients with DLBCL.


Subject(s)
Aged , Aged, 80 and over , Antineoplastic Combined Chemotherapy Protocols , Cyclophosphamide/therapeutic use , Doxorubicin/therapeutic use , Humans , Liposomes/therapeutic use , Lymphoma, Large B-Cell, Diffuse/drug therapy , Prednisolone , Prednisone/therapeutic use , Retrospective Studies , Rituximab/therapeutic use , Vincristine/therapeutic use
2.
Article in Chinese | WPRIM | ID: wpr-773700

ABSTRACT

The aim of this paper was to observe the concentration,time and mechanism of autophagy induced by triptolide( TP) in ovarian granulosa cells( OGCs). CCK-8 method was used to compare the inhibitory effects of TP at different concentrations on primary cultured rat OGCs and IC50 was calculated. The effects of TP at different concentrations and time points on the expression of OGCs autophagy factor protein and the cascade of PI3 K/AKT/m TOR pathway were detected by Western blot. The effects of TP,autophagy inducer( brefeldin A) and PI3 K/m TOR inhibitor( NVP-BEZ235) on the expression of PI3 K/AKT/m TOR cascade and autophagy related factor protein were detected by Western blot. The results show that the IC50 of different concentrations of TP on OGCs of rat ovary was14. 65 μmol·L-1,and the minimum inhibitory concentration of TP was 0. 1 μmol·L-1( 100 nmol·L-1). Compared with the control group,the expression levels of beclin1 and LC3Ⅱ in each group were significantly higher than those in the control group( P<0. 05 or P<0. 01). After 12 hours of treatment with TP,brefeldin A and NVP-BEZ235,respectively,compared with the control group,TP could significantly promote the expression level of downstream autophagy effect or molecule beclin1,LC3Ⅱ and inhibit the expression level of LC3Ⅰ,p62 protein( P<0. 05 or P< 0. 01). Moreover,the expression of beclin1 and LC3Ⅱ/LC3Ⅰ in TP group was higher than that in brefeldin A group( P<0. 05 or P<0. 01),and the expression of p62 in TP group was lower than that in brefeldin A group( P<0. 05 or P<0. 01). At the same time,TP could significantly inhibit the expression of p-PI3 K,p-AKT,p-mTOR protein,and the inhibitory effect of TP was better than that of NVP-BEZ235 group. This study suggests that 100 nmol·L-1 TP could induce OGCs autophagy successfully in cultured rat ovary for 12 h; TP may induce OGCs autophagy by inhibiting PI3 k/Akt/m TOR signaling pathway.


Subject(s)
Animals , Apoptosis , Autophagy , Cell Proliferation , Cells, Cultured , Diterpenes , Pharmacology , Epoxy Compounds , Pharmacology , Female , Granulosa Cells , Phenanthrenes , Pharmacology , Phosphatidylinositol 3-Kinases , Metabolism , Proto-Oncogene Proteins c-akt , Metabolism , Rats , Signal Transduction , TOR Serine-Threonine Kinases , Metabolism
3.
Article in Chinese | WPRIM | ID: wpr-657521

ABSTRACT

Objective To analyze the effect of psychological care intervention combined with aspirin and tigrine in patients with acute myocardial infarction and its effect on quality of life. Methods A total of 82 patients with acute myocardial infarction were enrolled in our hospital from March 2016 to April 2017. The patients were divided into control group by random number table method and 41 patients in the experimental group. The patients in the control group were treated with aspirin and tigrilil. The study group increased the psychological nursing intervention on the basis of routine treatment, and analyzed the clinical symptom remission rate, complication rate and quality of life of the two groups. Results The remission rate and quality of life of the clinical group were significantly higher than those of the control group, and the complication rate was significantly lower than that of the control group (P<0.05). Conclusion The treatment of patients with acute myocardial infarction with psychological care combined with aspirin and tiereliluo treatment, can improve the clinical symptoms of remission rate, reduce complications, but also improve the quality of life, worthy of clinical application.

4.
Article in Chinese | WPRIM | ID: wpr-657515

ABSTRACT

Objective To explore the effect of drug therapy in the treatment of acute ICU patients with acute myocardial infarction and the effect of psychological intervention. Methods A total of 120 patients with acute ICU acute myocardial infarction admitted to Ningbo Yinzhou People's Hospital from May 2016 to May 2017 were randomly divided into two groups: observation group and control group (60 cases). Two group were based on psychological care, respectively, the implementation of drug combination of psychological intervention and drug treatment, compared the two groups of patients with negative emotions, FMD, endothelin 1, NO. Results FMD (14.51±2.73)%, endothelin 1 (72.74±10.56) ng/L, NO (46.41±5.86) μmol/L, depression score (10.52±1.85), anxiety score (11.06±1.47) were better than those in the control group (P<0.05). Conclusion Diazepam combined with fluoxetine hydrochloride dispersible tablet and psychological intervention can improve the negative emotion of patients with acute ICU acute myocardial infarction.

5.
Article in Chinese | WPRIM | ID: wpr-311582

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the clinical features and treatment methods of refractory/relapsed diffuse large B cell lymphoma (DLBCL) patients, and to explore the curative effect and the main factors affecting prognosis.</p><p><b>METHODS</b>Clinical data of 1043 cases of DLBCL in our hospital from January 2008 to April 2016 were retrospectively analyzed, then the clinical data of 153 patients with refractory/relapsed lymphoma were selected and analyzed for determing the relationship of the related factors with therapeutic effect and prognosis. Treatment methods include chemotherapy alone and chemotherapy combined with radio-therapy, the first line regimen was CHOP or R-CHOP program, the salvage regimens are ICE, Hyper CVAD or EPOCH, etc. The median age of these 153 patients was 50 years old, the ratio of male and female was 1.59:1.</p><p><b>RESULTS</b>4 cases were not treated in 153 patients, 6 cases (4.03%) of 149 patients have been treated and achieved complete remission(CR), 18 cases (12.08%) achieved partial remission(PR), and the total response rate was 16.1%. Single factor analysis showed that the patients' serum LDH values, IPI score, bulky, extra-node involvement, bone marrow infiltration and the salvage regimen all could influence the survival rate, with statistically significantce (P<0.05).</p><p><b>CONCLUSION</b>Refractory/relapsed DLBCL mainly occurs in the middle-aged and male, the serum LDH value, IPI score, bulky and scope of lesions are mainly factors influencing the prognosis of refractory/relapsed DLBCL patients.</p>

6.
Article in Chinese | WPRIM | ID: wpr-659689

ABSTRACT

Objective To analyze the effect of psychological care intervention combined with aspirin and tigrine in patients with acute myocardial infarction and its effect on quality of life. Methods A total of 82 patients with acute myocardial infarction were enrolled in our hospital from March 2016 to April 2017. The patients were divided into control group by random number table method and 41 patients in the experimental group. The patients in the control group were treated with aspirin and tigrilil. The study group increased the psychological nursing intervention on the basis of routine treatment, and analyzed the clinical symptom remission rate, complication rate and quality of life of the two groups. Results The remission rate and quality of life of the clinical group were significantly higher than those of the control group, and the complication rate was significantly lower than that of the control group (P<0.05). Conclusion The treatment of patients with acute myocardial infarction with psychological care combined with aspirin and tiereliluo treatment, can improve the clinical symptoms of remission rate, reduce complications, but also improve the quality of life, worthy of clinical application.

7.
Article in Chinese | WPRIM | ID: wpr-659676

ABSTRACT

Objective To explore the effect of drug therapy in the treatment of acute ICU patients with acute myocardial infarction and the effect of psychological intervention. Methods A total of 120 patients with acute ICU acute myocardial infarction admitted to Ningbo Yinzhou People's Hospital from May 2016 to May 2017 were randomly divided into two groups: observation group and control group (60 cases). Two group were based on psychological care, respectively, the implementation of drug combination of psychological intervention and drug treatment, compared the two groups of patients with negative emotions, FMD, endothelin 1, NO. Results FMD (14.51±2.73)%, endothelin 1 (72.74±10.56) ng/L, NO (46.41±5.86) μmol/L, depression score (10.52±1.85), anxiety score (11.06±1.47) were better than those in the control group (P<0.05). Conclusion Diazepam combined with fluoxetine hydrochloride dispersible tablet and psychological intervention can improve the negative emotion of patients with acute ICU acute myocardial infarction.

8.
Chinese Journal of Pathophysiology ; (12): 1921-1931, 2017.
Article in Chinese | WPRIM | ID: wpr-667676

ABSTRACT

AIM:To investigate the effect of bone marrow mesenchymal stem cell(BMSC) transplantation on learning and memory abilities and pathological changes of Alzheimer disease (AD) mice and the molecular mechanisms. METHODS:C57/BL6 wild-type (WT) and transgenic(Tg) mice were randomly divided into 4 groups:WT/PBS group, WT/BMSCs group,Tg/PBS group and Tg/BMSCs group. The mice were administered with PBS or BMSCs via intracere-broventricular injection. Spatial learning and memory abilities of the mice were evaluated by Morris water maze test on the 3rd day after surgery. Real-time PCR was applied to detect the mRNA expression of CX3C chemokine ligand 1 (CX3CL1),CX3C chemokine receptor 1 (CX3CR1), IL-1β, TNF-α, Nurr1, YM1, insulin-degrading enzyme (IDE) and matrix metalloproteinase 9(MMP9). The protein levels of CX3CL1 and Aβ42 were measured by ELISA. Western blot was used to detect the protein expression of postsynaptic density protein 95 (PSD95) and synaptophysin (SYP). RE-SULTS:The transplanted BMSCs were observed near the hippocampus of APP/PS1 mice on the 10th postoperative day. The escape latency of the mice in Tg/PBS group was significantly longer than that in the WT/PBS mice(P<0.05). Com-pared with Tg/PBS group,the escape latency of Tg/BMSCs group was significantly shorter (P<0.05), and the mRNA and protein levels of CX3CL1 in Tg/BMSCs group were significantly higher than those in Tg/PBS group (P<0.01). The results of immunohistofluorescence staining showed that BMSC transplantation promoted the activation of microglia in the brain of WT and Tg mice. The mRNA expression of YM1 was up-regulated in WT/BMSCs group and Tg/BMSCs group (P<0.05). Compared with WT/PBS mice, the mRNA expression of TNF-α in the cortex and hippocampus of Tg/PBS group was significantly increased (P<0.05),and the mRNA expression of Nurr1 in the cortex was significantly decreased (P<0.01). Meanwhile,the mRNA expression of TNF-α in the cortex of Tg/BMSCs mice was decreased(P<0.01) and the mRNA expression of CX3CR1 and Nurr1 was up-regulated compared with Tg/PBS group (P<0.05). The results of Western blot showed that the protein levels of PSD95,p85,p110 and p-Akt in Tg/BMSCs group were significantly higher than those in Tg/PBS group (P<0.05). Finally, BMSC transplantation reduced the protein level of Aβ42 in APP/PS1 mice(P<0.05), and increased the mRNA expression of IDE and MMP9 in the hippocampus (P <0.05). CONCLU-SION:BMSC transplantation modulates neuroinflammatory responses and promotes neuroprotective factor and synaptic pro-tein expression,thus improving the learning and memory abilities in the APP/PS1 mice,which may be achieved by up-reg-ulating the expression of CX3CL1.

9.
Article in Chinese | WPRIM | ID: wpr-360067

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the clinical course of a very elderly patient with advanced diffuse large B cell lymphoma (DLBCL), so as to explore the incidence, prognosis and treatment of DLBCL and to analyse the prognostic and therapeutic significance of molecular subtype.</p><p><b>METHODS</b>The clinical history, auxiliary examinations, clinical diagnostic standards, therapeutic methods, biopsy and autopsy of this patient were retrospectively analyzed; the incidence, current treatment status, molecular biological features, and prognostic and therapeutic significance of molecular subtype were studied.</p><p><b>RESULTS</b>After admission, this patient was diagnosed as non-GCB DLBCL, NOS, stage IV B and in the high risk group (IPI = 5, ECOG = 2). She achieved a decent partial response after many times of imunochemotherapy, but his disease status soon progressed. The liver occupying biopsy revealed non-GCB, while the spleen tumor pathology revealed GCB; pathological typing of these two methods was completely opposite. Autopsy pathological diagnosis showed that the death causes included extensive tumor metastasis, dyscrasia and respiratory circle failure.</p><p><b>CONCLUSION</b>Incidence of aged patients with DLBCL is high, and the disease is aggressive; the treatment is low responsive and difficult, and new therapeutic methods are needed. Gene expression profile (GEP) can provide molecular subtype and potential pathogenic mechanism, which can promote the development of new targeted therapy and individualized treatment.</p>


Subject(s)
Aged , Female , Humans , Lymphoma, Large B-Cell, Diffuse , Diagnosis , Pathology , Therapeutics , Neoplasm Staging , Prognosis
10.
Article in Chinese | WPRIM | ID: wpr-272491

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the cytogenetic abnormalities and prognostic outcomes of patients with multiple myeloma (MM) detected by fluorescence in situ hybridization (FISH).</p><p><b>METHODS</b>The clinical record of 117 newly-diagnosed patients with MM treated in department of hematology and geriatric hematology of our hospital for 7 years were collected, and their molecular cytogenetic abnormalities detected by FISH and the clinical outcome were analyzed retrospectively.</p><p><b>RESULTS</b>The detected rate of cytogenetic abnormality was 76.9%(90/117), the most common abnormality deteted by FISH was 1q21+ (71.1%), followed by 13q- (56.6%). The cross comparison method showed that 13q- and 17p13-, t(11;14) and t(4;14) were related respectively. All the patients with cytogenetic abnormalities showed no significant difference in the overall survival from cytogenetic normal patients.</p><p><b>CONCLUSION</b>The positive rate of molecular cytogenetic abnormalities detected by FISH in MM patients is high, but data from larger and longer studies are needed to evaluate the prognostic outcomes.</p>


Subject(s)
Chromosome Aberrations , Chromosome Deletion , Cytogenetics , Humans , In Situ Hybridization, Fluorescence , Multiple Myeloma , Diagnosis , Genetics , Prognosis , Retrospective Studies , Translocation, Genetic
11.
Article in Chinese | WPRIM | ID: wpr-357310

ABSTRACT

Aged patients with acute myeloid leukemia accounted for more than half of the adult AML patients, which has characteristics of low complete remission rate, short overall survival and poor prognosis. Recently, the importance of cytogenetics in AML was gradually realized. The diagnosis and typing, prognosis stratification and guide for treatment are performed on the basis of cytogenetics, but the research on influence of cytogenetics on adged patients with AML are relatively few. This review briefly summarizes the prognostic significance of cytogenetics in aged patients with AML.


Subject(s)
Aged , Cytogenetic Analysis , Cytogenetics , Humans , Leukemia, Myeloid, Acute , Prognosis , Remission Induction
12.
Article in Chinese | WPRIM | ID: wpr-259633

ABSTRACT

<p><b>OBJECTIVE</b>This study was aimed to investigate the morphological, immunophenotype, cytogenetic characteristics, clinical and therapy features in one elderly patient with chronic lymphocytic leukemia (CLL) combined with invasive aspergillose infection(IAI).</p><p><b>METHODS</b>The morphological features of bone marrow cells from patient were observed by light microscope, the immunophenotype were detected by flow cytometry, the cytogenetic characteristics were assayed by conventional chromosomal analysis and fluorescence in situ hybridization (FISH).</p><p><b>RESULTS</b>at onset of disease, the patient was diagnosed as B-CLL, Rai stage is II. He was treated with a course of RF(fludarabine 50 mg×5, rituximab 600 mg×5) chemotherapy, and achived complete remission (CR) lasting for five years, then the patient was treated with multiple courses of chemotherapy and maintained at a steady state of disease. After the last chemotherapy, this patient developed a fever, his temperature fluctuated at 37.2-38.7°C, the lung CT showed the presence of massive shadow, repeated 1-3-β-D-glucan test was positive,and he was considered as invasive aspergillosis infection, voriconazole was intravenously injected him for 2 months, his lung CT showed better efficacy. However, the leukemia continued progress, his hemogram was extremely low, invasive aspergillosis infection relapsed,voriconazole treatment was poor effect,ultimately this patient died of the rapid progress of leukemia and multiple organ invasive aspergillosis. Autopsy showed chronic lymphocytic leukemia with multiple metastases and multiple organ invasive aspergillosis.</p><p><b>CONCLUSION</b>invasive aspergillosis is a serious complication for CLL patients,once occurs, the prognosis is poor,so early diagnosis and prophylactic antifungal therapy may reduce fungal infection complication.</p>


Subject(s)
Aged , Antibodies, Monoclonal, Murine-Derived , Antifungal Agents , Antineoplastic Combined Chemotherapy Protocols , Aspergillosis , Flow Cytometry , Humans , In Situ Hybridization, Fluorescence , Leukemia, Lymphocytic, Chronic, B-Cell , Male , Remission Induction , Rituximab , Vidarabine
13.
Article in Chinese | WPRIM | ID: wpr-264949

ABSTRACT

This study was purposed to evaluate the effectiveness and safety of autologous cytokine induced killer (CIK) cells combined with chemotherapy in treatment of elderly patients with acute myeloid leukemia. Peripheral blood mononuclear cells (PBMNC) were isolated from 5 elderly patients with acute myeloid leukemia, and then augmented by priming with interferon gamma (IFN-γ) followed by IL-2 and monoclonal antibody (mAb) against CD3. The autologous CIK cells thus obtained were infused back to individual patients, 28 days as one cycle. The changes in cellular immune function, incidence of infection, independence of hematoglobin or blood transfusion, and progression of disease were observed and assessed before and after therapy. The results showed that the 46 cycles of CIK cell infusion were performed for 5 patients, no adverse reaction was observed in these patients. The percentages of CD3(+), CD3(+)CD8(+) and CD3(+)CD56(+) increased significantly (P < 0.05), The therapy of CIK could significantly reduce the incidence of infection (P < 0.05) and shorten the time of high fever in AML patients (P < 0.05). CIK also could reduce the volume of erythrocyte infusion to maintenance hematoglobin level (P < 0.05). We found that although CIK could not change the outcome of AML, the combination of CIK and chemotherapy could control patients' condition and prolong their survival during the development and end stage of AML. It is concluded that autologous CIK cells combined with chemotherapy is safe and efficacious for the elderly patients with acute myeloid leukemia.


Subject(s)
Aged , Aged, 80 and over , Combined Modality Therapy , Cytokine-Induced Killer Cells , Humans , Leukemia, Myeloid, Acute , Drug Therapy , Therapeutics , Male
14.
Article in Chinese | WPRIM | ID: wpr-349722

ABSTRACT

DNA methylation of ID4 gene promoter occurred frequently in patients with acute leukemia and was found to be highly related to the tumor progression. Due to lack of the appropriate methylation detection methods, the relation between the quantification of ID4 methylation and the states of acute leukemia is still unclear. This study purposed to set up a methylation-specific quantitative PCR system for ID4 and investigate the specificity and sensitivity of this methylation detection. The plasmids combined with target gene as well as with internal reference were constructed, and the standard curves were set up by using above mentioned plasmids. The specificity of this detection system in cell lines was verified through techniques of MSP and quantitative MSP. The sensitivity of this detection system was verified by mixing methylation-positive and negative cell lines in varying proportions and through amplification of qualitative MSP. The results showed that the standard curves were establish successfully. The results of quantitative MS-PCR in cell lines were consistent with those of MS-PCR, and as low as 1: 10(-5) of ID4 methylation positive cells could be detected by the new methylation detection assay. In newly diagnosed acute leukemia patients, the positive rate of quantitative MSP was higher. It is concluded that a complete quantitative MSP system for ID4 methylation detection has been established and this quantitative MSP method has good specificity and high sensitivity.


Subject(s)
Acute Disease , Cell Line, Tumor , DNA Methylation , Humans , Inhibitor of Differentiation Proteins , Genetics , Leukemia , Genetics , Polymerase Chain Reaction , Methods , Sensitivity and Specificity
15.
Article in Chinese | WPRIM | ID: wpr-349717

ABSTRACT

Imatinib has been recognized as the frontline therapy drug in chronic myeloid leukemia (CML), however, only limited patients could achieve complete molecular remission (CMR). Recent clinical and basic proofs indicated an improved treatment outcome by the combination of interferon and Imatinib. This study was purposed to evaluated systematically the efficacy and safety of interferon plus Imatinib in patients with CML. Data from relative clinical trials were from clinical trial of gov and Cochrane Collaboration. A comprehensive literature search was performed from data bases such as pubMed and EM. The results indicated that 7 clinical trials and 12 research papers met the criteria enrolled in study, included 697 cases in total. The combination group had higher complete cytogenetic remission (CCgR) rate than imatinib alone at 6 months (58% vs 42%; P = 0.0001) and 12 months (74% vs 68%; P = 0.004). The major molecular remission (MMR) rate was also higher in the combination group at 6 months (58% vs 34%; P = 0.0001) and 12 months (66% vs 47%; P < 0.0001). Furthermore, compared with single drug, the combination group had superior CMR rate at 6 months (13% vs 2%; P = 0.0002) and 12 months (14% vs 5%; P = 0.0009). The major adverse effects of combination therapy were rash, asthenia, edema and musculoskeletal events, and combination therapy was more prone to inducing neutropenia, thrombocytopenia and mild anemia. It is concluded that compared with Imatinib alone, the combination of interferon and Imatinib has better clinical efficacy in treating CML with earlier cytogenetic and molecular remission. It is also a safe therapy in spite of slightly weaker tolerance than single drug therapy.


Subject(s)
Antineoplastic Combined Chemotherapy Protocols , Therapeutic Uses , Benzamides , Humans , Imatinib Mesylate , Interferons , Leukemia, Myelogenous, Chronic, BCR-ABL Positive , Drug Therapy , Piperazines , Pyrimidines , Treatment Outcome
16.
Article in Chinese | WPRIM | ID: wpr-349649

ABSTRACT

The advances of treatment improved the prognosis of the patients with acute leukemia (AL) in the last decade, but the lack of general biomarker for predicting relapse in AL, which is one of the most important factors influencing the survival and prognosis. DNA methylation of ID4 gene promoter occurred frequently in patients with AL and was found to be highly related to the tumor progression. Based on the previous work of the setup of methylation-specific quantitative PCR system for ID4 gene, this study was designed to investigate the relation between the quantitative indicator of methylation density, percentage of methylation reference(PMR) value, and different disease status of AL. PMR of ID4 was detected by MS-PCR in bone marrow (BM) samples of 17 healthy persons and 54 AL patients in the status of newly diagnosis, complete remission and disease relapse. The results showed that at different disease status, PMR value in newly diagnosed group was significantly lower than that in complete remission group (P = 0.031). Among serial samples, PMR value remained very low at the status of patients with continuous complete remission (<1.5‰), and increased along with the accumulation of tumor cells at relapse. In 1 relapse case, the abnormal rise of PMR value occurred prior to morphological relapse. PMR value seemed to be related to body tumor cell load. It is concluded that the quantitative indicator of methylation density and PMR value may reflect the change of tumor cell load in acute leukemia patients. Dynamic monitoring of PMR maybe predict leukemia relapse.


Subject(s)
Acute Disease , Adolescent , Adult , Aged , Aged, 80 and over , Bone Marrow , Case-Control Studies , DNA Methylation , Female , Humans , Inhibitor of Differentiation Proteins , Genetics , Leukemia , Genetics , Male , Middle Aged , Polymerase Chain Reaction , Methods , Young Adult
17.
International Eye Science ; (12): 2223-2225, 2014.
Article in Chinese | WPRIM | ID: wpr-637026

ABSTRACT

AlM:To determine the relations between the changes of vascular endothelial growth factor ( VEGF ) concentration in plasma, aqueous humor and vitreous of patients with diabetic retinopathy ( DR ) and proliferative diabetic retinopathy ( PDR) , as well as to find if the condition of metabolism control of patients with DR may affect the VEGF level. METHODS: Double antibody sandwich enzyme-linked immunosorbent assay was used to measure the levels of VEGF concentration in plasma of non-diabetic retinopathy ( NDR ) patients group, background diabetic retinopathy ( BDR ) patients group, and proliferative diabetic retinopathy ( PDR) patients group. The levels of HbAlc in plasma of patients in above three groups were measured at the same time. The VEGF concentration in aqueous humor, vitreous of PDR patients group and normal group was also measured for further comprehensive analysis. Human VEGF immunoassay Quantikine with high sensibility and quality was imported from R&D Co. LTD. , USA. RESULTS: There was an increasing tendency in the VEGF concentration in aqueous humor of PDR patients group. There was no statistically significant difference as it was compared with normal group (P>0. 05). The VEGF concentration in vitreous of PDR patients group was obviously increased, there was significant difference as it was compared with normal group (P 0. 05 ). ln normal group the VEGF concentration in plasma was the highest, but in PDR patients group the VEGF concentration in vitreous was the highest. CONCLUSlON: The significantly increased VEGF concentration in eyes, especially in vitreous of PDR patients may play an important role in development and deterioration of DR. lt was clear that VEGF played its normal biological functions mainly in plasma in normal human. ln severe DR patients, abnormal high may come from ischemic and VEGF concentration in vitreous suggested that it hypoxic retina and there was a tendency to diffuse towards the front of eyes.

18.
Article in Chinese | WPRIM | ID: wpr-302362

ABSTRACT

The purpose of this study was to explore the association between X-ray repair cross-complementing group 1 (XRCC1)gene polymorphism and non-Hodgkin's lymphoma risk. A total of 282 non-Hodgkin's lymphoma (NHL) patients and 231 normal controls were used to investigate the effect of three XRCC1 gene polymorphisms (rs25487, rs25489, rs1799782) on susceptibility to non-Hodgkin's lymphoma. Genotyping was performed by using SNaPshot method. All statistical analyses were done with R software. Genotype and allele frequencies of XRCC1 were compared between the patients and controls by using the chi-square test. Crude and adjusted odd ratios and 95% confidence intervals were calculated by using logistic regression on the basis of genetic different models. For four kinds of NHL, subgroup analyses were also conducted. Combined genotype analyses of the three XRCC1 polymorphisms were also done by using logistic regression. The results showed that the variant genotype frequency was not significantly different between the controls and NHL or NHL subtype cases. Combined genotype analyses of XRCC1 399-280-194 results showed that the combined genotype was not associated with risk of NHL overall, but the VT-WT-WT combined genotype was associated with the decreased risk of T-NHL (OR: 0.21; 95%CI (0.06-0.8); P = 0.022), and the WT-VT-WT combined genotype was associated with the increased risk of FL(OR:15.23; 95%CI (1.69-137.39); P = 0.015). It is concluded that any studied polymorphism (rs25487, rs25489, rs1799782) alone was not shown to be rela-ted with the risk of NHL or each histologic subtype of NHL. The combined genotype with mutation of three SNP of XRCC1 was not related to the risk of NHL. However, further large-scale studies would be needed to confirm the association of decreased or increased risk for T-NHL and FL with the risk 3 combined SNP mutants of XRCC1 polymorphism.


Subject(s)
Case-Control Studies , China , Epidemiology , DNA Repair , DNA-Binding Proteins , Genetics , Female , Humans , Lymphoma, Non-Hodgkin , Epidemiology , Genetics , Male , Middle Aged , Polymorphism, Single Nucleotide , Risk Factors , X-ray Repair Cross Complementing Protein 1
19.
Journal of Experimental Hematology ; (6): 1464-1470, 2013.
Article in Chinese | WPRIM | ID: wpr-264994

ABSTRACT

The purpose of this study was to explore the clinicopathological features, therapy and prognostic factors of elderly patients with non-Hodgkin's lymphoma (NHL). The clinical data including general clinical characteristics, pathological features, chemotherapy selection and treatment response of 30 patients with NHL in our hospital from January 2003 to December 2012 were analyzed retrospectively. The survival was analyzed by using Kaplan-Meier methods, and the prognosis was evaluated by COX regression multivariate analysis model. The clinical parameters selected include age, Ann Arbor stage, international prognostic index (IPI), B symptom and lactate dehydrogenase (LDH) levels. The results showed that all the patients suffered from underlying disease, and the cardiovascular disease (hypertension, coronary heart disease, arrhythmia) is the most common, and minority (8/30) combined with secondary tumor, the 63% (19/30) cases had B symptoms at diagnosis. only 2 cases were diagnosed as T-cell lymphoma; the 93% (28/30) cases combined with B-cell lymphoma, 57% (17/28) of them combined with diffuse large B-cell lymphoma. Ann-Arbor stage ≤ IIwas 37% (11/30);10(37%) patient's IPI score was ≤ 2, and 67% (20/30) was scored 3-5; 13(43%) patient's serum LDH level was abnormal. Modified R-CHOP chemotherapy was given individually on the basis of clinical features. The patients achieved complete remission, partial remission, stable disease, or progressive disease accounted for 14 (46.7%), 13 (43.3%), 1 (3.3%), and 2 (6.7%), respectively; the total reaction rate was 90% after 4 cycles of chemotherapy; the overall survival (OS) rate at 1 and 2 years was 73.3% and 43.3%, and progression-free survival (PFS)rate at 0.5 and 1 years was 62.2% and 54.9%; multivariate analysis by COX regression showed that B symptoms and Ann-Arbor stage were independent factors (P = 0.014, 0.039; RR = 6.678, 4.939, respectively) affecting the OS of elderly NHL, and IPI score affected PFS independently. It is concluded that elderly patients with NHL usually are of late stage at newly diagnosis and have suffered from underlaying diseases. Besides strengthening supportive treatment, modified R-CHOP chemotherapy should be given individually according to different prognosis. B symptoms and Ann-Arbor stage >II are indicators for poor prognosis of elderly NHL.


Subject(s)
Aged , Aged, 80 and over , Female , Humans , Lymphoma, Non-Hodgkin , Diagnosis , Pathology , Therapeutics , Male , Middle Aged , Prognosis , Retrospective Studies , Treatment Outcome
20.
Article in Chinese | WPRIM | ID: wpr-325211

ABSTRACT

This study was aimed to evaluate the effectiveness and safety of low methylation drug decitabine combined with autologous cytokine induced killer cells (CIK) to treat the elderly patients with acute myeloid leukemia (AML). Two AML patients aged over 80 years old were diagnosed and treated in our department from 2006 to 2012; both company with MDS history, and one case was M4-type, another case was M6-type according to FAB classification. The changes in lymphocyte subsets, hematologic response, transfusion frequency, leukemic gene expression, obtaining CR or PR, quality of life and survival time of the patients with different treatment regimen (decitabine alone; CIK alone; decitabine combined with CIK) were systematically observed. The results showed that therapy of decitabine combined with CIK cells could reduce bone marrow suppression extent, decrease the frequency and volume of blood transfusion, and prolong the duration of partial remission, compared with the single use of CIK cell infusion and single use of decitabine treatment. Meanwhile, the kinds of expressed genes associated with leukemia decreased and the survival time was prolonged obviously. The patients' life quality significantly improved. It is concluded that decitabine combined with CIK for treatment of elderly patients with AML is safe and effective.


Subject(s)
Aged, 80 and over , Azacitidine , Therapeutic Uses , Cytokine-Induced Killer Cells , Humans , Immunotherapy, Adoptive , Leukemia, Myeloid, Acute , Drug Therapy , Therapeutics , Male , Treatment Outcome
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