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1.
Article in Chinese | WPRIM | ID: wpr-877619

ABSTRACT

The evolution from "vessel dominated by heart" of "heart dominating pericardium meridian of hand-


Subject(s)
Acupuncture Points , Hand , Meridians , Moxibustion , Pericardium
2.
Article in Chinese | WPRIM | ID: wpr-879417

ABSTRACT

OBJECTIVE@#To study the relationship between osteonecrosis of femoral head after internal fixation of femoral neck fracture and serum bone metabolism, vascular active factors, and analyze the risk factors.@*METHODS@#Total 150 patients with femoral neck fracture who underwent reduction and internal fixation from April 2016 to April 2019 were selected, including 83 males and 67 females. According to whether there was necrosis of femoral head after operation, they were divided into necrosis group(32 cases) and non necrosis group (118 cases). Before operation and 1, 3, 5 days after operation, the serum levels of beta-C terminal cross-linked telopeptides of typeⅠ collagen(β-CTX), N-telopeptide of typeⅠ procollagen(PINP), nitric oxide (NO), Endothelin-1 (ET-1) were measured. The clinical characteristics of the two groups were compared. The risk factors of postoperative femoral head necrosis were analyzed by logistic regression model. The value of serum indexes in predicting postoperative femoral head necrosis was analyzed by ROC curve.@*RESULTS@#There was no significant difference in the levels of serum PINP and β-CTX between necrotic group and non necrotic group before operation and 1, 3 and 5 days after operation(@*CONCLUSION@#The content of serum NO and ET-1 on the first day after operation can predict the necrosis of femoral head.


Subject(s)
Female , Femoral Neck Fractures/surgery , Femur Head , Femur Head Necrosis/etiology , Fracture Fixation, Internal/adverse effects , Humans , Male , Osteonecrosis , Retrospective Studies , Risk Factors
3.
Article in Chinese | WPRIM | ID: wpr-837872

ABSTRACT

Objective To investigate the dietary and nutritional status of submarine crew, and to provide guides for reasonable diet and reference for seting new food standards for military personal. Methods During submarine docking, 100 submarine crew were selected as subjects. A total of 100 questionnaires were sent out and 96 valid questionnaires were collected, with an effective rate of 96.0%. The dietary survey was carried out for 4 consecutive days by weighing method. The daily energy consumption of the crew was measured by 24-hour life observation method, meanwhile the body mass index (BMI) and body fat rate were also measured. The common nutritional deficiency symptoms and suggestions on food supply were evaluated by the questionnaire. Results The survey showed that the food intakes, such as cereals, soybean, vegetables, poultry, milk, fish and shrimp, were insufficient, while the intakes of livestock meat and vegetable oil were above the military standard. Their daily energy intakes were higher than the military standard requirements and could meet the energy consumption; the protein and fat energy supply ratios were too high, while that of carbohydrate was too low. The intakes of sodium, iron, phosphorus and vitamin E were all over the standard, while the intakes of iodine, vitamin A and B vitamins were insufficient. Physical investigation showed that overweight and obesity was 46.8% (36/77), and slightly higher and high body fat rate was 48.1% (37/77). In addition, the incidences of nutritional deficiency symptoms, such as dry eyes, bleeding gums, and mouth and tongue ulcers were 26.0% (25/96), 22.9% (22/96) and 19.8% (19/96), respectively. Fruits, dairy products, beef and mutton were the top three recommended food to be increased for the crew's food supply. Conclusion The submarine crew have an unreasonable dietary structure, inappropriate energy supply of the three major nutrients, and unbalanced nutrient intakes. It is necessary to adjust the dietary structure and improve the diet pattern so as to promote the crew's health..

4.
Article in English | WPRIM | ID: wpr-773376

ABSTRACT

OBJECTIVE@#To investigate the molecular characteristics and intracellular growth ability of Legionella pneumophila (L. pneumophila) strains from 1989 to 2016 in Sichuan Province, China.@*METHODS@#Seventy-nine isolates of L. pneumophila were collected from environmental and clinical sources, including cooling towers, hot springs, bath water, fountains, and patients, and identified with 16S rRNA gene analysis and serum agglutination assay. The isolates were then typed by Sequence-Based Typing (SBT), and Genotyping of forty-two LP1 strains were analyzed by means of multiple-locus VNTR analysis with 8 loci (MLVA-8). All strains were further analyzed for two virulence genes: Legionella vir homologue (lvh) and repeats in structural toxin (rtxA). The intracellular growth ability of 33 selected isolates was determined by examining their interaction with J774 cells.@*RESULTS@#All isolates were identified to L. pneumophila including 11 serogroups, among which the main serogroup were LP1, accounting for 54.43%. Thirty-three different sequence types (STs) from five main clonal groups and five singletons were identified, along with 8 different MLVA patterns. Both the lvh and rtxA loci were found in all 79 strains. Thirty isolates showed high intracellular growth ability in J774 cells.@*CONCLUSION@#L. pneumophila is a potential threat to public health, and effective control and prevention strategies are urgently needed.


Subject(s)
Bacterial Proteins , Genetics , Bacterial Toxins , Genetics , China , Genotyping Techniques , Humans , Legionella pneumophila , Genetics , RNA, Ribosomal, 16S , Genetics , Water Microbiology
5.
Article in Chinese | WPRIM | ID: wpr-905732

ABSTRACT

This study described the significance of professional education in physical therapy in Capital Medical University School of Rehabilitation Medicine under the background of the World Confederation for Physical Therapy (WCPT) International Course Accreditation, analyzed the present problems of core curriculum system as well as the inevitable trends of carrying on the education reform of physical therapy, and the continuous reform program of core curriculum system in physical therapy based on the accreditation of the WCPT were suggested. The final purpose was to improve the quality of physical therapy talents substantially in order to achieve the standard of WCPT accreditation.

6.
Chinese Medical Journal ; (24): 435-439, 2018.
Article in English | WPRIM | ID: wpr-342020

ABSTRACT

<p><b>Background</b>The pathogenesis of postural tachycardia syndrome (POTS) remains unclear. This study aimed to explore the changes and significance of sulfur dioxide (SO) in patients with POTS.</p><p><b>Methods</b>The study included 31 children with POTS and 27 healthy children from Peking University First Hospital between December 2013 and October 2015. A detailed medical history, physical examination results, and demographic characteristics were collected. Hemodynamics was recorded and the plasma SOwas determined.</p><p><b>Results</b>The plasma SOwas significantly higher in POTS children compared to healthy children (64.0 ± 20.8 μmol/L vs. 27.2 ± 9.6 μmol/L, respectively, P < 0.05). The symptom scores in POTS were positively correlated with plasma SOlevels (r = 0.398, P < 0.05). In all the study participants, the maximum heart rate (HR) was positively correlated with plasma levels of SO(r = 0.679, P < 0.01). The change in systolic blood pressure from the supine to upright (ΔSBP) in POTS group was smaller than that in the control group (P < 0.05). The ΔSBP was negatively correlated with baseline plasma SOlevels in all participants (r = -0.28, P < 0.05). In the control group, ΔSBP was positively correlated with the plasma levels of SO(r = 0.487, P < 0.01). The change in HR from the supine to upright in POTS was obvious compared to that of the control group. The area under curve was 0.967 (95% confidence interval: 0.928-1.000), and the cutoff value of plasma SOlevel >38.17 μmol/L yielded a sensitivity of 90.3% and a specificity of 92.6% for predicting the diagnosis of POTS.</p><p><b>Conclusions</b>Increased endogenous SOlevels might be involved in the pathogenesis of POTS.</p>

7.
Tianjin Medical Journal ; (12): 728-732, 2018.
Article in Chinese | WPRIM | ID: wpr-809751

ABSTRACT

@#Objective Todiscusstheclinicaleffectandtechnicalmethodofthesurgicaltreatmentforpotentially resectablecancerofpancreaticbody-tail.Methods Elevenpatientswithpotentiallyresectablecancerofpancreaticbodytailoradjacentorganmetastasis,whounderwentwideexcisionandpostoperativechemotherapyinourdepartmentwere retrospectivelyanalyzed. Results Inelevenpatients,10patientswithadjacentorganmetastasiswereperformedinvolved organsresection,3patientsweregivenresectionoftheceliacaxis(CA)andcommonhepaticartery(CHA)and2patients weregiventotalgastrectomyandjejunalinterpositionpouchreconstruction.Onepatienthadprolongedhospitalstaydueto incisioninfection,butnoseriouscomplicationsoccurredinotherpatients.Exceptfor2patients,theotherpatientsunderwent postoperativechemotherapyofgemcitabine.Allofthepatientsachievedreliefthroughoperation.Themediansurvivaltime was 28 (14, 36) months. One and 3-year survival rates were 90.9% and 36.3%. Conclusion The resection rate of potentiallyresectablecarcinomaofpancreaticbodyandtailoperationislower,andpatientshavelongbeenplaguedby abdominal pain. The treatment of extensive resection operation and recessive resection and postoperative adjuvant chemotherapycanimprovethequalityoflifeandprolongthesurvivalrateofpatients.

8.
Article in Chinese | WPRIM | ID: wpr-696420

ABSTRACT

Vasovagal syncope(VVS),with its relatively high morbidity,is one of the most common types of au-tonomic-mediated reflex syncope in children,which can seriously affect pediatric patients in their life and study.A se-ries of research have shown that the mechanism for VVS is closely related to autonomic nervous imbalance,neurohor-monal factor,and cerebral blood flow abnormality,and has a certain degree of genetic tendency.Now the progress in the mechanisms for VVS at home and abroad is reviewed,in order to provide certain help for further research.

9.
Chinese Medical Journal ; (24): 2778-2784, 2017.
Article in English | WPRIM | ID: wpr-324740

ABSTRACT

<p><b>BACKGROUND</b>Vasovagal syncope (VVS) is the most common cause of syncope in children. Neuropeptide Y (NPY) plays an important role in the regulation of blood pressure (BP), as well as myocardial contractility. This study aimed to explore the role of plasma NPY in VVS in children.</p><p><b>METHODS</b>Fifty-six children who were diagnosed with VVS (VVS group) using head-up tilt test (HUT) and 31 healthy children who were selected as controls (control group) were enrolled. Plasma NPY concentrations were detected. The independent t-test was used to compare the data of the VVS group with those of the control group. The changes in plasma NPY levels in the VVS group during the HUT, as well as hemodynamic parameters, such as heart rate (HR), BP, total peripheral vascular resistance (TPVR), and cardiac output (CO), were evaluated using the paired t-test. Furthermore, the correlations between plasma NPY levels and hemodynamic parameters were analyzed using bivariate correlation analysis.</p><p><b>RESULTS</b>The BP, HR, and plasma NPY (0.34 ± 0.12 pg/ml vs. 0.46 ± 0.13 pg/ml) levels in the supine position were statistically low in the VVS group compared to levels in the control group (all P < 0.05). Plasma NPY levels were positively correlated with the HR (Pearson, R = 0.395, P < 0.001) and diastolic BP (Pearson, R = 0.311, P = 0.003) when patients were in the supine position. When patients in the VVS group were in the supine position, elevated TPVR (4.6 ± 3.7 mmHg·min-1·L-1 vs. 2.5 ± 1.0 mmHg·min-1·L-1, respectively, P < 0.001; 1 mmHg = 0.133 kPa) and reduced CO (1.0 ± 0.7 L/min vs. 2.4 ± 1.3 L/min, respectively, P < 0.001) were observed in the positive-response period compared with baseline values. The plasma NPY levels were positively correlated with TPVR (Spearman, R = 0.294, P = 0.028) but negatively correlated with CO in the positive-response period during HUT (Spearman, R = -0.318, P = 0.017).</p><p><b>CONCLUSIONS</b>Plasma NPY may contribute to the pathogenesis of VVS by increasing the TPVR and decreasing the CO during orthostatic regulation.</p>

10.
Acta Pharmaceutica Sinica ; (12): 620-624, 2017.
Article in Chinese | WPRIM | ID: wpr-779638

ABSTRACT

A rapid fluorescence polarization immunoassay (FPIA) has been developed for the determi-nation of aflatoxins in samples of naturally-contaminated herbal teas. The tracers were synthesized by chemical method and determined by thin layer chromatography (TLC) and mass spectroscopy (MS). Fluorescence polarization was evaluated by the detection of polarized light. The results showed that the limit of detection (LOD) of FPIA for aflatoxins was 20 ng·mL-1, the IC50 was 371.80 ng·mL-1, and the linear range of the developed FPIA was 92.76-252.32 ng·mL-1. Compared with conventional HPLC methods, the FPIA developed in this study has the advantages of short analysis time and low cost. This method may be suitable for high- throughput screening of aflatoxins in herbal teas.

11.
Article in English | WPRIM | ID: wpr-812451

ABSTRACT

Insulin resistance is the pathophysiological basis of many diseases. Overcoming early insulin resistance highly significant in prevention diabetes, non-alcoholic fatty liver, and atherosclerosis. The present study aimed at evaluating the therapeutic effects of baicalin on insulin resistance and skeletal muscle ectopic fat storage in high fat diet-induced mice, and exploring the potential molecular mechanisms. Insulin resistance in mice was induced with a high fat diet for 16 weeks. Animals were then treated with three different doses of baicalin (100, 200, and 400 mg·kg(-1)·d(-1)) for 14 weeks. Fasting blood glucose, fasting serum insulin, glucose tolerance test (GTT), insulin tolerance test (ITT), and skeletal muscle lipid deposition were measured. Additionally, the AMP-activated protein kinase/acetyl-CoA carboxylase and protein kinase B/Glycogen synthase kinase 3 beta pathways in skeletal muscle were further evaluated. Baicalin significantly reduced the levels of fasting blood glucose and fasting serum insulin and attenuated high fat diet induced glucose tolerance and insulin tolerance. Moreover, insulin resistance was significantly reversed. Pathological analysis revealed baicalin dose-dependently decreased the degree of the ectopic fat storage in skeletal muscle. The properties of baicalin were mediated, at least in part, by inhibition of the AMPK/ACC pathway, a key regulator of de novo lipogenesis and activation of the Akt/GSK-3β pathway, a key regulator of Glycogen synthesis. These data suggest that baicalin, at dose up to 400 mg·kg(-1)·d(-1), is safe and able to attenuate insulin resistance and skeletal muscle ectopic fat storage, through modulating the skeletal muscle AMPK/ACC pathway and Akt/GSK-3β pathway.


Subject(s)
AMP-Activated Protein Kinases , Metabolism , Acetyl-CoA Carboxylase , Metabolism , Adipose Tissue , Metabolism , Animals , Diet, High-Fat , Flavonoids , Pharmacology , Glycogen Synthase Kinase 3 beta , Physiology , Insulin Resistance , Male , Mice , Mice, Inbred C57BL , Muscle, Skeletal , Metabolism , Proto-Oncogene Proteins c-akt , Physiology , Signal Transduction , Physiology
12.
Article in Chinese | WPRIM | ID: wpr-243439

ABSTRACT

<p><b>OBJECTIVE</b>To observe the dynamic expression of calcium-sensing receptor(CaSR) in myocardium of diabetic rats.</p><p><b>METHODS</b>Thirty male Wistar rats were randomly divided into 3 groups including control, diabetic-4 week and diabetic-8 week groups(n = 10). The type 2 diabetes mellitus models were established by intraperitoneal injection of streptozotocin (STZ, 30 mg/kg) after high-fat and high-sugar diet for one month. The cardiac morphology was observed by electron microscope. Western blot analyzed the expression of CaSR, phospholamban (PLN), a calcium handling regulator, and Ca+-ATPase(SERCA) in cardiac tissues.</p><p><b>RESULTS</b>Compared with control group, the expressions of CaSR and SERCA were decreased, while the expression of PLN was significantly increased in a time-dependent manner in diabetic groups. Meanwhile diabetic rats displayed abnormal cardiac structure.</p><p><b>CONCLUSION</b>These results indicate that the CaSR expression of myocardium is reduced in the progression of DCM, and its potential mechanism may be related to the imnaired intracellular calcium homeostasis.</p>


Subject(s)
Animals , Calcium-Binding Proteins , Metabolism , Diabetes Mellitus, Experimental , Diabetes Mellitus, Type 2 , Diabetic Cardiomyopathies , Metabolism , Disease Progression , Heart , Male , Myocardium , Metabolism , Pathology , Rats , Rats, Wistar , Receptors, Calcium-Sensing , Metabolism , Sarcoplasmic Reticulum Calcium-Transporting ATPases , Metabolism , Streptozocin
13.
Article in Chinese | WPRIM | ID: wpr-329850

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the clinical features of pandrug-resistant Acinetobacter baumannii (PDR-Ab) in the Chinese PLA General Hospital and compare the efficacies of different antibiotic treatments in aged patients with ventilator-associative pneumonia (VAP) caused by PDR-Ab.</p><p><b>METHODS</b>Data were collected from all isolated PDR-Ab strains in our hospital from April 2009 to April 2010. The clinical features, treatment, and outcomes were retrospectively reviewed.</p><p><b>RESULTS</b>PDR-Ab was found to be the dominant pathogen in 42 of 126 aged VAP patients. Cefoperazone/sulbactam plus minocycline showed good efficacy in 20 patients with PDR-Ab VAP, showing a clinical cure rate of 65% (13/20) and a bacterial eradication rate of 40% (8/20). Another 22 patients were treated with other antimicrobial drugs, achieving a clinical cure rate of 22.7% (5/22) and a bacterial eradication rate of 13.6% (3/22). The factors influencing bacterial clearance were prolonged length of hospital stay and mechanical ventilation prior to positive culture (all P<0.01).</p><p><b>CONCLUSION</b>Cefoperazone/sulbactam plus minocycline can be an effective treatment for VAP caused by PDR-Ab.</p>


Subject(s)
Acinetobacter baumannii , Aged, 80 and over , Anti-Bacterial Agents , Therapeutic Uses , Cefoperazone , Therapeutic Uses , Drug Resistance, Multiple, Bacterial , Drug Therapy, Combination , Female , Humans , Male , Minocycline , Therapeutic Uses , Pneumonia, Ventilator-Associated , Drug Therapy , Microbiology , Retrospective Studies , Sulbactam , Therapeutic Uses , Treatment Outcome
14.
Chinese Journal of Virology ; (6): 429-435, 2014.
Article in Chinese | WPRIM | ID: wpr-280347

ABSTRACT

This study aimed to construct recombinant adenovirus expressing Epstein-Barr nuclear antigen 1 (EBNA1) against nasopharyngeal carcinoma (NPC). The C-terminal region fragment of the ebna1 gene of Epstein-Barr virus was amplified from the standard strain B95-8 by polymerase chain reaction (PCR). The gene fragment was inserted into the pDC316 shuttle plasmid using the EcoRI and BgIII restriction enzyme sites. The pDC316-ebna1 shuttle plasmid and pBHG helper plasmid were cotransfected into HEK293 cells after sequencing. The soluble protein was extracted from HEK293 cells, which caused apparent cytopathic effects. The transcription and expression of the ebna1 gene were confirmed using flow cytometry and Western blotting. rAd-ebna1 titers were measured by the TCID50. rAd-ebna1 was injected into BALB/c mice at a dose of 2 x 10(8) VP per mouse, EBNA1 epitope-specific responses were measured at 1st, 2nd, 4th and 8th weeks post-immunization. The target fragment of ebna1 (939 bp) was obtained by PCR, and was in consensus with the sequence from the standard strain B95-8. Cytopathic effects were observed after the pDC316-ebna1 shuttle plasmid and pBHG helper plasmid were cotransfected into HEK293 cells. rAd-ebna1 was successfully recombined in HEK293 cells. EBNA1 protein was detected in HEK293 cells, rAd-ebna1 titers reached 10(8) TCID50/mL. Specific responses to CD4+ epitopes of EBNA1 were detected in the immunized mice. In conclusion, rAd-ebna1 was successfully constructed and induced specific responses to CD4+ epitopes of EBNA1 in immunized mice.


Subject(s)
Adenoviridae , Genetics , Allergy and Immunology , Animals , CD4-Positive T-Lymphocytes , Allergy and Immunology , Virology , Epstein-Barr Virus Infections , Allergy and Immunology , Virology , Epstein-Barr Virus Nuclear Antigens , Genetics , Allergy and Immunology , Genetic Vectors , Genetics , Allergy and Immunology , Herpesvirus 4, Human , Genetics , Allergy and Immunology , Humans , Male , Mice , Mice, Inbred BALB C , Viral Proteins , Genetics , Allergy and Immunology
15.
Article in Chinese | WPRIM | ID: wpr-318059

ABSTRACT

<p><b>OBJECTIVE</b>To screen aptamers that can bind P24 antigen tightly and specificly, and verify its specificity and affinity.</p><p><b>METHODS</b>Polycarbonate PCR plate was coated with P24 antigen and SELEX technology was used to screen aptamer on the PCR plate. The primary and secondary structure of these aptamers was analyzed by software. Through HRP labeled streptavidin and biotin labeled aptamers, the affinity and specificity of obtained aptamers were verified by ELISA.</p><p><b>RESULTS</b>The polycarbonate PCR plate could be coated with P24 antigen. Electrophoretic analysis showed the aptamers had been enriched. Sequence aligment analysis showed that these aptamers have consensus sequence and their apatial structure was multiple; ELISA verified that aptamers had high affinity with P24 antigen.</p><p><b>CONCLUSION</b>A simple method was established for screening aptamers that can bind HIV-1 P24 antigen specificly and tightly.</p>


Subject(s)
HIV Core Protein p24 , HIV-1 , Allergy and Immunology , Humans , Polymerase Chain Reaction , SELEX Aptamer Technique , Methods
16.
Article in Chinese | WPRIM | ID: wpr-287529

ABSTRACT

<p><b>OBJECTIVE</b>To study the mechanism of treating diabetic foot by clearing heat, detoxification, activating blood, and dredging collaterals method.</p><p><b>METHODS</b>Sixty diabetic foot patients were randomly assigned to the treatment group and the control group, 30 cases in each group. On the basis of the same routine treatment, patients in the treatment group were treated by Qingjie Tongluo Recipe (QTR) plus external washing of Chinese herbs plus external dressing by herbs with removing necrosis and promoting granulation actions, while those in the control group were treated with routine aseptic external dressing. Three months was taken as one therapeutic course. The wound area and basic fibroblast growth factor (bFGF) were detected before and after treatment. The content of vascular endothelial growth factor (VEGF), the peripheral vascular and nerve functions were also measured. The therapeutic effects were also observed.</p><p><b>RESULTS</b>After treatment, in the treatment group,15 patients were cured, 12 markedly effective, 2 effective, 1 ineffective, the cure rate was 50.0% and the total effective rate was 96.7%, while in the control group, 9 cases were cured, 6 markedly effective, 8 effective, 7 ineffective, the cure rate was 30.0% and the total effective rate was 76.7%. The total effective rate was better in the treatment group than in the control group (P <0. 01). The contents of bFGF and VEGF were significantly higher in the two groups after treatment (P <0.01). Besides, better results were obtained in the treatment group (P < 0.01). The blood flow speed of the dorsalis pedis artery, the inner diameter of the dorsalis pedis artery, and the common peroneal nerve conduction velocity were somewhat improved (P <0.05, P <0.01). Besides, better results were obtained in the treatment group (P <0.01).</p><p><b>CONCLUSIONS</b>QTR combined external washing plus external dressing by herbs with removing necrosis and promoting granulation actions could promote the healing of diabetic foot induced ulcers, improve the vascular and nerve functions. Its efficacy was superior to that of the control group.</p>


Subject(s)
Aged , Diabetic Foot , Drug Therapy , Drugs, Chinese Herbal , Therapeutic Uses , Female , Humans , Male , Middle Aged , Phytotherapy
17.
Article in Chinese | WPRIM | ID: wpr-235377

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect of dopamine receptor (DR2) activation on hypoxia/reperfusion injury (HRI) in the neonatal rat cardiomyocytes, and to explore its mechanism.</p><p><b>METHODS</b>The hypoxia/reperfusion (H/R) injury model was established in primarily cultured neonatal rat cardiomyocytes, and randomly assigned: control, H/R, bromocriptine (Bro) and haloperidol (Hal) groups. The cell apoptosis was detected using inverted microscope, transmission electron microscope and flow cytometry (FCM). The lactate dehydrogenase(LDH) and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in cell medium were analyzed. The expression of mRNA and protein of caspase-3, caspase-8, caspase-9, Fas, Fas-L, Cyt C and Bcl-2 were detected by RT-PCR and Western blot, respectively.</p><p><b>RESULTS</b>Compared with the control group, apoptosis rate, LDH activity, MDA content and the expression of pro-apoptotic factors and anti-apoptotic factors were increased, but SOD activity was decreased in H/R group. Compared with the H/R group, all index above-mentioned were down-regulated or reversed in Bro-group, and had no obvious differences in Hal-group.</p><p><b>CONCLUSION</b>The neonatal rat cardiomyocytes injury and apoptosis caused by hypoxia/reperfusion can be inhibited with DR2 activation, which mechanism is related to scavenging oxygen radical.</p>


Subject(s)
Animals , Animals, Newborn , Apoptosis , Cell Hypoxia , Myocardial Reperfusion Injury , Metabolism , Myocytes, Cardiac , Cell Biology , Metabolism , Oxidative Stress , Rats , Rats, Wistar , Receptors, Dopamine D2 , Metabolism
18.
Journal of Medical Biomechanics ; (6): E210-E215, 2013.
Article in Chinese | WPRIM | ID: wpr-804213

ABSTRACT

Objective To make the stent expand uniformly along the axial, an improved kriging optimization algorithm is applied for to the optimal design of stent-balloon system to find the optimal length of the balloon. Methods Based on finite element method results, kriging surrogate model combining with Latin hypercube sampling (LHS) approach and expected improvement (EI) function was employed for the optimization of balloon length to reduce stent dog-boning effect during its dilation. Results The kriging surrogate model could approximately establish the relationship between stent dog-boning rate and balloon length and so to replace the expensive reanalysis of stent dilation. Sample points from LHS could perfectly represent the vector space. EI function could be used to effectively find out the global optimal solution with high probability. The optimal length of balloon could make the stent expand uniformly. Conclusions This adaptive optimization method based on kriging surrogate model can optimize the design of stent–balloon system effectively.

19.
Article in Chinese | WPRIM | ID: wpr-345650

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the risk factors for hearing impairment in premature infants.</p><p><b>METHODS</b>A total of 895 premature infants who were admitted to the neonatal intensive care unit from January to December 2010 were evaluated using distortion product otoacoustic emission to detect hearing impairment. The failure rates in initial screening and secondary screening were recorded. The risk factors for failure to pass hearing screenings were elucidate using multivariate logistic regression analysis.</p><p><b>RESULTS</b>The failure rate in initial screening was 38.4%, and the failure rate in secondary screening was 18.3%. In the auditory brainstem response test conducted at three months after birth, the failure rate was 22.2%. In premature infants with a gestational age of 28-29(+6) weeks, 60.5% did not pass the initial screening; 48.1% of the premature infants with a birth weight of 1 001-1 499  g failed the initial screening; 70.0% of the premature infants with a birth weight of ≤1 000  g failed the initial screening; 53.8% of the premature infants who had severe asphyxia failed the initial screening; 45.0% of the premature infants who used invasive ventilation failed the initial screening; 47.9% of the premature infants with a total bilirubin of ≥340 µmol/L failed the initial screening; 54.6% of the premature infants with septicemia failed the initial screenings. The multivariate logistic regression analysis revealed the following independent risk factors for failing the initial and secondary hearing screenings: gestational age, birth weight, hyperbilirubinemia and septicemia.</p><p><b>CONCLUSIONS</b>Premature infants are susceptible to hearing impairment because they have immature organs and tissues and incomplete blood-brain barrier function and are sensitive to such factors as hyperbilirubinemia and infection. Early hearing screening and follow-up are necessary for premature infants to ensure timely interventions.</p>


Subject(s)
Evoked Potentials, Auditory, Brain Stem , Female , Hearing Loss , Humans , Infant, Newborn , Infant, Premature , Infant, Premature, Diseases , Logistic Models , Male , Neonatal Screening , Risk Factors
20.
Article in Chinese | WPRIM | ID: wpr-316615

ABSTRACT

<p><b>OBJECTIVE</b>To explore the plausibility of laryngeal preservation in salvage surgery of recurrent laryngeal carcinoma.</p><p><b>METHODS</b>Comprehensive clinical and radiological assessments on a specific group of patients with recurrent laryngeal cancer and their relapsed tumors were carried out to determine the methods and indications of salvage surgery for preservation of laryngeal functions. Salvage surgery with preservation of larynx was performed in a selective of 36 laryngeal cancer patients with relapsed tumors in their larynges after radiotherapy or partial laryngectomies. All patients were regularly followed up after discharge from the hospital. Postoperative survival was calculated by Kaplan-Meier's method.</p><p><b>RESULTS</b>Among various laryngeal preservation regimens given, a second transoral laser surgery was carried out in 4 cases, horizontal hemilaryngectomy in 4 cases, vertical hemilaryngectomy in 13 cases and Majer-Piquet's operation in 15 cases, respectively. Simultaneous neck dissection was conducted in 18 cases. Postoperative complications were encountered in 6 cases, most common of which were local infection and laryngeal fistula with no hospital mortality and other major morbidities. Five cases with immediate postoperative complications were cured with proper management. The remained one case undergone vertical partial laryngectomy for recurrence after tumor resection via laryngeal fissure received postoperative radiation due to positive surgical margin, and complicated laryngeal atresia and obstruction with loss of his laryngeal functions. Local recurrence was observed in 5 cases and simultaneous locoregional recurrence was seen in 1 case, with the resulting local control rate of 83.3% (30/36). The 3-year postoperative survival rate was 80.6%. The cause of death was tumor recurrence in 3 cases, distant metastasis in 3 cases and non-tumor-associated disease (heart attack) in 1 case, respectively.</p><p><b>CONCLUSIONS</b>Salvage surgery with laryngeal preservation can be carried out in the selected cases with early or locally-confined recurrent lesions within the larynx, on the condition that the recurrent tumors be assessed thoroughly and indications for associated surgical techniques be evaluated properly.</p>


Subject(s)
Adult , Aged , Carcinoma, Squamous Cell , Pathology , General Surgery , Humans , Laryngeal Neoplasms , Pathology , General Surgery , Laryngectomy , Methods , Male , Middle Aged , Neoplasm Recurrence, Local , General Surgery , Salvage Therapy , Treatment Outcome
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