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1.
Article in English | WPRIM | ID: wpr-929245

ABSTRACT

Four new sesquiterpene quinone meroterpenoids, dysideanones F-G (1-2) and dysiherbols D-E (3-4), were isolated from the marine sponge Dysidea avara collected from the South China Sea. The new structures were elucidated by extensive analysis of spectroscopic data including HR-MS and 1D and 2D NMR spectra, and their absolute configurations were assigned by single-crystal X-ray diffraction and ECD calculations. Anti-inflammatory evaluation showed that dysiherbols D-E (3-4) exhibited moderate inhibitory activity on TNF-α-induced NF-κB activation in human HEK-293T cells with IC50 values of 10.2 and 8.6 μmol·L-1, respectively.


Subject(s)
Animals , Dysidea/chemistry , Porifera , Quinones/pharmacology , Sesquiterpenes/pharmacology , Skeleton
2.
Article in Chinese | WPRIM | ID: wpr-879884

ABSTRACT

OBJECTIVE@#To study brainstem auditory evoked potential (BAEP) in neonates with hyperbilirubinemia using short auditory stimuli (60 dBnHL), and to investigate the differences in the inter-aural latency difference (ILD) of wave V between neonates with different total serum bilirubin (TSB) levels.@*METHODS@#A prospective study was conducted in neonates with hyperbilirubinemia who were admitted to the Department of Neonatology, Yuhuan People's Hospital of Zhejiang Province, from May 2019 to October 2020. The neonates were divided into a severe group (@*RESULTS@#Compared with the mild group, the severe group had significantly higher proportions of neonates with abnormal hearing threshold and abnormal ILD (@*CONCLUSIONS@#Serum bilirubin in neonates affects the ILD of BAEP wave V, especially in those with severe hyperbilirubinemia. ILD at the optimal cut-off value of ≥0.4 ms shows potential value in the diagnosis of hearing impairment caused by neonatal hyperbilirubinemia.


Subject(s)
Bilirubin , Evoked Potentials, Auditory, Brain Stem , Hearing Loss , Humans , Hyperbilirubinemia , Hyperbilirubinemia, Neonatal , Infant, Newborn , Prospective Studies
3.
Article in Chinese | WPRIM | ID: wpr-873187

ABSTRACT

Objective:To observe the effect of Xiao Jianzhongtang on Adenylate-activated protein kinase/peroxidase proliferation-activated receptor coactivator 1-α (AMPK/PGC1-α) signaling pathway in skeletal muscle of exercise fatigue mice.Method:Forty Kunming mice were randomly divided into normal group, model group, Buzhong Yiqitang group and Xiao Jianzhongtang group, with 10 mice in each group. The model group, Buzhong Yiqitang group and Xiao Jianzhongtang group were trained on the treadmill to establish a fatigue model, and the normal group did not apply any intervention. At the same time as the treadmill training, the model group was given the same amount of normal saline. Xiao Jianzhongtang was administered with 5 g·kg-1 of medicine, and Buzhong Yiqitang was administered with 2.8 g·kg-1 of medicine for 6 days. After the experiment, the weight of each group of mice and the time of running out of exhaustion were measured,the colorimetric method was used to detect the serum urea (UREA), lactate dehydrogenase (LDH), muscle glycogen (MG), and skeletal muscle of each group of mice Na+-K+-ATPase, Ca2+-Mg2+-ATPase content, pathological changes of skeletal muscle of each group were observed by hematoxylin-eosin (HE) staining, Western blot was used to detect the protein expression of AMPK and PGC1-α in skeletal muscle of each group .Result:Compared with normal group, the body weight of model group significantly decreased (P<0.01), and the contents of Na+-K+-ATPase, Ca2+-Mg2+-ATPase, LDH, and MG significantly decreased (P<0.05,P<0.01). The content of UREA increased significantly (P<0.01), and the expression of AMPK and PGC1-α protein increased significantly (P<0.01). Compared with model group, the mice in the Xiao Jianzhongtang group had significantly increased body weight (P<0.05), significantly increased the time spent on treadmill exhaustion(P<0.01), Na+-K+-ATPase, Ca2+-Mg2+-ATPase, LDH, and MG. The content increased significantly(P<0.05, P<0.01), the content of UREA decreased significantly (P<0.01), and the expression of AMPK and PGC1-α protein increased significantly (P<0.01).Conclusion:Xiao Jianzhongtang has an anti-exercise fatigue effect, which may be related to enhancing skeletal muscle AMPK/PGC1-α pathway,enhancing mitochondrial oxidative phosphorylation,reducing accumulation of metabolites,slowing down glycogen consumption and decomposition,and enhancing skeletal muscle energy synthesis.

4.
Article in Chinese | WPRIM | ID: wpr-872757

ABSTRACT

Objective:To investigate the protective effect of Wutou Chishizhi Wan on myocardial ischemia reperfusion injury (MIRI) in rats, and observe its effect on such mechanisms as coagulation function, vascular endothelial cells and oxidative stress in rats. Method:A total of 40 SD rats were randomly divided into normal group, model group, positive drug group (Urokinase group) and Wutou Chishizhi Wan group, with 10 rats in each group. Except for the normal group, rat myocardial ischemia-reperfusion injury models were established. The changes of heart rate (HR) at 10 min before ischemia, 30 min after ischemia and 30, 60, 120 min (T0,T1,T2,T3,T4), and the change of electrocardiogram (ECG) J point after modeling in rats were observed. The pathological changes of rat myocardial tissue were observed by hematoxylin-eosin (HE) staining. The changes of four indexes of coagulation [prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen content decreased significantly (FIB)] in rats were observed. The contents of endothelin-1 (ET-1), thromboxane A2 (TXA2) and prostacyclin (PGI2) in serum and myocardium levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) of MIRI rats were observed. Western blot assay was used for the detection of oxidative stress protein Keap1 and transcription factor-E2-related factor (Nrf2) expression levels in rat myocardial tissue. Result:Compared with the normal group, the ECG of MIRI rats showed significant myocardial ischemic injury-like changes, ST segment was significantly elevated, J point was significantly increased, and the incidences of HR in T1, T2, T3 and T4 were significantly reduced (P<0.05, P<0.01). Compared with the model group, Wutou Chishizhi Wan significantly reduced ECG J-point changes in MIRI rats, while increased the incidence of HR in T1, T2, T3 and T4 (P<0.05, P<0.01). Compared with the normal group, PT, APTT and TT in the model group were significantly shortened (P<0.01), FIB content was significantly increased (P<0.01), and the serum PGI2 level decreased and TXA2 and ET-1 levels increased significantly in the model group (P<0.01). SOD content and GSH-Px activities of myocardial tissue in the model group were significantly reduced (P<0.01), whereas the MDA content was increased (P<0.01). Compared with the model group, PT of the Wutou Chishizhi Wan group was prolonged (P<0.05) and APTT slightly prolonged, TT significantly prolonged (P<0.01), FIB content decreased (P<0.05), serum PGI2 increased (P<0.05), TXA2 and ET-1 decreased significantly in the Wutou Chishizhi Wan group (P<0.01), myocardial MDA content decreased, and SOD content and GSP-Px activity increased significantly (P<0.01). Meanwhile, the Wutou Chishizhi Wan group was able to activate the Keap1/Nrf2 signaling pathway, which significantly increased Nrf2 expression and significantly decreased Keap1 expression (P<0.01). Conclusion:Wutou Chishizhi Wan group can protect myocardial injury in MIRI rats. The specific mechanism is to protect MIRI by regulating vascular endothelial cell homeostasis and oxidative stress levels and activating Keap1/Nrf2 signaling pathway.

5.
Chinese Journal of Hepatology ; (12): 148-152, 2020.
Article in Chinese | WPRIM | ID: wpr-787635

ABSTRACT

To analyze the clinical characteristics of cases of novel coronavirus pneumonia and a preliminary study to explore the relationship between different clinical classification and liver damage. Consecutively confirmed novel coronavirus infection cases admitted to seven designated hospitals during January 23, 2020 to February 8, 2020 were included. Clinical classification (mild, moderate, severe, and critical) was carried out according to the diagnosis and treatment program of novel coronavirus pneumonia (Trial Fifth Edition) issued by the National Health Commission. The research data were analyzed using SPSS19.0 statistical software. Quantitative data were expressed as median (interquartile range), and qualitative data were expressed as frequency and rate. 32 confirmed cases that met the inclusion criteria were included. 28 cases were of mild or moderate type (87.50%), and four cases (12.50%) of severe or critical type. Four cases (12.5%) were combined with one underlying disease (bronchial asthma, coronary heart disease, malignant tumor, chronic kidney disease), and one case (3.13%) was simultaneously combined with high blood pressure and malignant tumor. The results of laboratory examination showed that the alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), and total bilirubin (TBil) for entire cohort were 26.98 (16.88 ~ 46.09) U/L and 24.75 (18.71 ~ 31.79) U/L, 39.00 (36.20 ~ 44.20) g/L and 16.40 (11.34- ~ 21.15) mmol/L, respectively. ALT, AST, ALB and TBil of the mild or moderate subgroups were 22.75 (16.31- ~ 37.25) U/L, 23.63 (18.71 ~ 26.50) U/L, 39.70 (36.50 ~ 46.10) g/L, and 15.95 (11.34 ~ 20.83) mmol/L, respectively. ALT, AST, ALB and TBil of the severe or critical subgroups were 60.25 (40.88 ~ 68.90) U/L, 37.00 (20.88 ~ 64.45) U/L, 35.75 (28.68 ~ 42.00) g/L, and 20.50 (11.28 ~ 25.00) mmol/L, respectively. The results of this multicenter retrospective study suggests that novel coronavirus pneumonia combined with liver damage is more likely to be caused by adverse drug reactions and systemic inflammation in severe patients receiving medical treatment. Therefore, liver function monitoring and evaluation should be strengthened during the treatment of such patients.

6.
Chinese Medical Journal ; (24): 1681-1688, 2019.
Article in English | WPRIM | ID: wpr-802626

ABSTRACT

Background@#Brachydactyly, a developmental disorder, refers to shortening of hands/feet due to small or missing metacarpals/metatarsals and/or phalanges. Isolated brachydactyly type E (BDE), characterized by shortened metacarpals and/or metatarsals, consists in a small proportion of patients with Homeobox D13 (HOXD13) or parathyroid-hormone-like hormone (PTHLH) mutations. BDE is often accompanied by other anomalies that are parts of many congenital syndromes. In this study, we investigated a Chinese family presented with BDE combined with pectus carinatum and short stature.@*Methods@#A four-generation Chinese family was recruited in June 2016. After informed consent was obtained, venous blood was collected, and genomic DNA was extracted by standard procedures. Whole-exome sequencing was performed to screen pathogenic mutation, array comparative genomic hybridization (Array-CGH) analysis was used to analyze copy number variations, and quantitative real-time polymerase chain reaction (PCR), stride over breakpoint PCR (gap-PCR), and Sanger sequencing were performed to confirm the candidate variation.@*Results@#A 3.06-Mb deletion (chr12:25473650–28536747) was identified and segregated with the phenotype in this family. The deletion region encompasses 23 annotated genes, one of which is PTHLH which has been reported to be causative to the BDE. PTHLH is an important regulator of endochondral bone development. The affected individuals showed bilateral, severe, and generalized brachydactyly with short stature, pectus carinatum, and prematurely fusion of epiphyses. The feature of pectus carinatum has not been described in the PTHLH-related BDE patients previously.@*Conclusions@#The haploinsufficiency of PTHLH might be responsible for the disease in this family. This study has expanded the knowledge on the phenotypic presentation of PTHLH variation.

7.
Article in Chinese | WPRIM | ID: wpr-802144

ABSTRACT

Objective: To clone CDS sequence of Dioscorea opposita SUS gene and analyze the protein structure,in order to provide a theoretical basis for the regulation mechanism of SUS gene and the synthesis mechanism of D.opposita polysaccharides.Method: Total RNA in D.opposita was extracted and reverse-transcribed into first strand of cDNA.Specific primers were designed according to an annotated SUS gene sequence obtained from the laboratory D.opposita genome database,and the coding region of the SUS gene was obtained by gene cloning technique and the protein sequence characteristics were analyzed by protein prediction analysis software.Result: A 2 448 bp gene sequence was cloned with a complete open reading frame (ORF).The gene was named DoSUS1.The formula of protein encoded by DoSUS1 gene in D.opposita was C4209H6534N1115O1205S23,and the molecular weight was 9 788.32,the total number of amino acids was 815,the theory isoelectric point (PI) was 6.10,the extinction coefficient was 110 505,the aliphatic index (AI) was 94.15,the instability index was 32.18,and the grand average of hydropathicity (GRAVY) was-0.225.It was a stable and soluble acidic protein.There were phosphorylation sites in the DoSUS1 amino acid sequence,with no transmembrane region and signal peptide.The secondary structure and the tertiary structure showed that DoSUS1 was an α class protein.Functional domain predictions showed that DoSUS1 had sucrose synthesis domain and glycosyl transfer domain.The homology alignment showed that the amino acid sequence of DoSUS1 was more than 80% similar to the amino acid sequence of the aligned monocots.The phylogenetic tree showed that DoSUS1 was closely related to SUS of monocotyledon evolution.Conclusion: The coding sequence of SUS gene was cloned from D.opposita for the first time,and its protein structure was analyzed to lay a foundation for further studying the roles of SUS in the growth and polysaccharide synthesis of D.opposita.

8.
Acta Physiologica Sinica ; (6): 725-731, 2019.
Article in Chinese | WPRIM | ID: wpr-777138

ABSTRACT

The purpose of this study was to investigate the anti-injury effect and protective mechanism of hydrogen-enriched water in a rat model of acute liver injury induced by aflatoxin B (AFB). Healthy male Sprague-Dawley (SD) rats were randomly divided into control group, model group (AFB group) and hydrogen-enriched water treatment group (AFB+H group). The rat model of acute liver injury induced by AFB was established by single intragastric administration of AFB (2.0 mg/kg), and then the rats were treated with hydrogen-enriched water intragastrically. HE staining was used to observe the pathological changes of liver tissue. Blood samples were taken from vena cava to measure serum liver function indexes. Live tissue was sampled to detect malondialdehyde (MDA) and reduced glutathione (GSH) contents. Western blot was used to detect phosphorylation levels of MAPK signaling pathway proteins (ERK, JNK and p38 MAPK). The results showed that, compared with the AFB group, the AFB+H group exhibited increased body weights, alleviated acute liver injury, decreased activities of serum glutamic-pyruvic transaminase and glutamic oxaloacetic transaminase, as well as total bilirubin level in the serum. Meanwhile, hydrogen-enriched water decreased MDA content and increased GSH content in liver tissue. AFB-increased phosphorylation levels of ERK, JNK and p38 MAPK in liver tissue were down-regulated significantly by hydrogen-enriched water treatment. These results suggest that hydrogen-enriched water can alleviate liver injury induced by AFB, and its mechanism may be related to the reduction of oxidative stress and the inhibition of MAPK signal transduction pathway activation.


Subject(s)
Aflatoxin B1 , Animals , Chemical and Drug Induced Liver Injury , Pathology , Deuterium Oxide , Therapeutic Uses , Liver , Pathology , MAP Kinase Signaling System , Male , Oxidative Stress , Rats , Rats, Sprague-Dawley
9.
Chinese Medical Journal ; (24): 1681-1688, 2019.
Article in English | WPRIM | ID: wpr-771172

ABSTRACT

BACKGROUND@#Brachydactyly, a developmental disorder, refers to shortening of hands/feet due to small or missing metacarpals/metatarsals and/or phalanges. Isolated brachydactyly type E (BDE), characterized by shortened metacarpals and/or metatarsals, consists in a small proportion of patients with Homeobox D13 (HOXD13) or parathyroid-hormone-like hormone (PTHLH) mutations. BDE is often accompanied by other anomalies that are parts of many congenital syndromes. In this study, we investigated a Chinese family presented with BDE combined with pectus carinatum and short stature.@*METHODS@#A four-generation Chinese family was recruited in June 2016. After informed consent was obtained, venous blood was collected, and genomic DNA was extracted by standard procedures. Whole-exome sequencing was performed to screen pathogenic mutation, array comparative genomic hybridization (Array-CGH) analysis was used to analyze copy number variations, and quantitative real-time polymerase chain reaction (PCR), stride over breakpoint PCR (gap-PCR), and Sanger sequencing were performed to confirm the candidate variation.@*RESULTS@#A 3.06-Mb deletion (chr12:25473650-28536747) was identified and segregated with the phenotype in this family. The deletion region encompasses 23 annotated genes, one of which is PTHLH which has been reported to be causative to the BDE. PTHLH is an important regulator of endochondral bone development. The affected individuals showed bilateral, severe, and generalized brachydactyly with short stature, pectus carinatum, and prematurely fusion of epiphyses. The feature of pectus carinatum has not been described in the PTHLH-related BDE patients previously.@*CONCLUSIONS@#The haploinsufficiency of PTHLH might be responsible for the disease in this family. This study has expanded the knowledge on the phenotypic presentation of PTHLH variation.

10.
Article in Chinese | WPRIM | ID: wpr-707098

ABSTRACT

Objective To establish fingerprint of Jinsangqi Kangdu Dropping Pills by HPLC; To control the quality of the preparation. Methods Waters XSELECT CSH-C18 chromatographic column (4.6 mm × 150 mm, 5 μm) was used and eluted with acetonitrile - 0.1% phosphoric acid solution gradient at the flow rate of 1.0 mL/min. The detection wavelength was 260 nm with column temperature of 30 ℃. Using calycosin-7-O-β-D-glucopyranoside, rutin, liquiritin, hyperoside, quercetin and ammonium glycyrrhizinate as the object references, ten batches of Jinsangqi Kangdu Dropping Pills were tested and analyzed by similarity comparison.Results Fringerprint spectrum of Jinsangqi Kangdu Dropping Pills had 24 common peaks in total, and characteristic spectrums of Hypericum Perforatum, Mori Cortex, Astragali Radix and Glycyrrhizae Radix et Rhizoma had been found, while similarity of HPLC fingerprint was more than 0.9 among those batches of samples. Conclusion Using HPLC fingerprint can evaluate the Jinsangqi Kangdu Dropping Pills quality totality,which can provide references for improving the quality control of the preparation.

11.
Article in Chinese | WPRIM | ID: wpr-693373

ABSTRACT

Objective Using substituted benzyl piperazine as the raw material to design and synthesize new piperazine deriva-tives with protein tyrosine kinase(PTK)inhibitory activity. Methods Benzoic acid was used as starting compound to synthesize a key intermediate,2-chloroethyl benzoate,and the target compounds were synthesized by further reaction of the key intermediate with different substituted benzyl piperazine derivatives.Enzyme-linked immunosorbent assay(ELISA)was used to test the PTK inhibitory activity of the compounds.Results Fifteen new compounds were synthesized and their structures were verified by IR,1H NMR,MS, and elemental analysis.The PTK inhibitory activity of 3h and 3o was stronger than that of the other compounds.Conclusion The syn-thetic method is simple,and the raw materials are cheap and readily available.Compounds 3h and 3o showed relatively higher PTK in-hibitory activities.

12.
Article in Chinese | WPRIM | ID: wpr-710142

ABSTRACT

AIM To study the correlations between colors and contents of nine active constituents of Salvia miltiorrhiza Bge.with four growth years (one,two,three and five years).METHODS The chromatic values (L*,a* and b*) were determined by precise color difference meter.HPLC was applied to determining the contents of water-soluble constituents (lithospermic acid,salvianolic acid B,rosmarinic acid,protocatechuic aldehyde acid and salvianic acid A sodium) and liposoluble-soluble constituents (dihydrotanshinone Ⅰ,cryptotanshinone,tanshinone Ⅰ and tanshinone Ⅱ A).Then the correlations between colors and contents of various constituents were investigated by Pearson correlation analysis.RESULTS L* and b * values of two growth-year S.miltiorrhiza,together with a* value of three growth-year S.miltiorrhiza,were the highest,but various chromatic values of five growth-year S.miltiorrhiza were all the lowest.The contents of various constituents in three growth-year S.miltiorrhiza reached the highest.Lithospermic acid and salvianic acid A sodium showed no significant correlations with various chromatic values,salvianolic B,rosmarinic acid and tanshinone Ⅱ A exhibited significantly positive correlations with them,and protocatechuic aldehyde had significantly negative correlations with them.CONCLUSION The quality of three growth-year S.miltiorrhiza is relatively high.

13.
Chinese Mental Health Journal ; (12): 971-977, 2017.
Article in Chinese | WPRIM | ID: wpr-703958

ABSTRACT

Objective:To explore the components and time characteristics of attentional bias in type 2 diabetic patients with different levels of self-management.Methods:The patients were first divided into higher,medium and lower self-management levels based on the Summary of Diabetes Self-care Activities Questionnaire (SDSCA),63 subjects in each group.A probe detection task was used to examine the impact of the different emotional pictures and stimulus presenting time on attentional bias.Results:When the stimuli were displayed for 500 ms,patients with higher self-management levels showed shorter reaction time to positive pictures than to negative pictures [(597.8 ± 185.5) ms vs.(626.0 ± 186.6) ms,P < 0.01],and their scores of negative attentional bias [(-22.5 ± 79.0) ms,P <0.05] and negative orienting index were lower than 0[(-26.6 ±74.5) ms,P <0.01].The scores of negative disengaging index were significant higher than 0 in patients with medium self-management levels [(17.2 ± 60.3) ms,P <0.05],the scores of positive disengaging index were significant lower than 0 in patients with lower selfmanagement levels [(-22.6 ±74.8) ms,P <0.05]].When the stimuli were displayed for 1250 ms,the scores of positive orienting index were higher than 0 in patients with medium self-management levels [(14.9 ± 54.4) ms,P < 0.05].Conclusion:It suggests that there are different characteristics of implicit cognitive processing in patients with different levels of self-management.

14.
Article in Chinese | WPRIM | ID: wpr-275458

ABSTRACT

This paper clarified in detail the definition, characteristics of allelopathy and its association with consecutive monoculture problem.Most of studies have indicated that it is critical to parse the formation mechanisms of consecutive monoculture problem that identification of allelochemicals and verification of its function. Here, we proposed a new approach to separate and identify the allelochemical group precisely and effectively, in which the "knock-out/in" methods of targeting ingredients in the model of medicinal effect identification and quality control were applied. This method will contribute to deep understanding plant allelopathy, and provide theoretical basis and technical support for alleviating consecutive monoculture problems simultaneously.

15.
Article in Chinese | WPRIM | ID: wpr-845734

ABSTRACT

Objective To elevated the decontamination properties of commercial nanoscale metal oxides against chemical warfare agents (CWA), and provide more foundation for the satisfactory materials of CWA decontamination. Methods Some nanocrystals of commercial metal oxides such an MgO, TiO2, ZnO and zinc nickel ferrite compound had been chosen to compare their decontamination properties. The nanocrystals were mixed with three representative compounds, sulfur mustard (HD), soman (GD) and SI 2-diisopropylaminoethyl) O-ethyl methylphosphonothioate (VX) at room temperature and natural light. The analogous experiments were conducted without addition of nanocrystals as negative control. After a fixed time, the samples were then analyzed by the methods of T-135, Schoeneman reaction and conversion method to determine the content of CWA. The decontamination properties of nanocrystals were compared with negative control. Results The chosen nanoscale metal oxides excepted nanoscale MgO had good decontamination properties against HD, and they all could decontaminate GD quickly. Nanoscale TiO2 had superior decontamination properties against GD and HD. At the room temperature and natural light, HD was completely decontaminated within 20 hours and GD was completely decontaminated within 4 hours by nanoscale TiO2. The nanocrystals of metal oxides didn't decontaminate VX effectively. Compared to the activated clay group, nanoscale MgO had superior decontamination properties against VX over other nanocrystals (P<0.05), but the percentage of degradation was lower than 20% within 7 h. Conclusion The chosen nanoscale TiO2 has superior decontamination properties against GD and HD than others in natural condition, but it isn't a promising agent for the decontamination of VX.

16.
Article in Chinese | WPRIM | ID: wpr-330216

ABSTRACT

To explore the effective components represented by fingerprint contributed to allelopathic effect of different Salvia miltiorrhiza aqueous concentration on seeds and seedlings of radish, grey relational analysis was used to establish the chromatography-efficacy relation. The results show that 15 peaks devote high allelopathic contribution to radish seeds and seedlings. The study will provide a new concept for allelochemicals screening and study.


Subject(s)
Allelopathy , Chromatography, High Pressure Liquid , Pheromones , Metabolism , Pharmacology , Plant Extracts , Metabolism , Pharmacology , Raphanus , Salvia miltiorrhiza , Chemistry , Metabolism
17.
Article in English | WPRIM | ID: wpr-820640

ABSTRACT

OBJECTIVE@#To compare the expression levels of pluripotent genes among incomplete reprogrammed colonies and induced pluripotent stem cells (iPSCs), to explore the relationship between the expression of pluripotent genes and incomplete reprogramming.@*METHODS@#Four genes (Oct4, Sox2, Klf4, C-Myc) were introduced into human foreskin fibroblasts (HFFs) by retroviruses. The HFFs were induced to reprogramming. Different forms of colonies were picked up, analyzed, and compared with iPSCs from different aspects, including the morphology of clones, alkaline phosphatase (AP) staining, immuno-fluorescence, and Q-PCR.@*RESULTS@#In the reprogramming process, different colonies were emerged, some of them exhibited typical human embryonic stem cell morphology (eg., compact colonies, high nucleus-to-cytoplasm ratios, and prominent nucleoli). However, these colonies couldn't maintain these characters after passage. There was an intermediate state, named partially reprogramming. Through analysis and identification, AP staining results were weakly positive, compared with iPSC colonies. The immuno-fluorescence staining demonstrated these colonies just expressed pluripotent protein Oct4. Q-PCR indicated that the expression of exogenous transcription factors was inappropriate, either at a high level or at a low level. Most of the endogenous pluripotency genes were expressed at a low level.@*CONCLUSIONS@#It may be one of the causes of incomplete reprogramming that the exogenous pluripotent gene is low-expressed or over-expressed, and successful reprogramming may depend on a specific stoichiometric balance of Oct4, Sox2, Klf4 and c-Myc.


Subject(s)
Animals , Cell Line , Cells, Cultured , Cellular Reprogramming , Genetics , Child , Fibroblasts , Humans , Induced Pluripotent Stem Cells , Cell Biology , Physiology , Male , Mice , Mice, Inbred ICR , Octamer Transcription Factor-3 , Genetics , Metabolism , Retroviridae , Genetics , Stage-Specific Embryonic Antigens , Genetics , Metabolism , Transfection , Methods
18.
Article in Chinese | WPRIM | ID: wpr-232209

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the efficiency of multiplex ligation-dependent probe amplification (MLPA) combined with short tandem repeat (STR) linkage analysis for the prenatal diagnosis for Duchenne muscular dystrophy (DMD).</p><p><b>METHODS</b>Gender of the fetus was first determined by the presence of Y chromosome sex-determining gene (SRY). Subsequently, combined MLPA and STR linkage analysis were applied for the probands, pregnant women and fetuses in 45 affected families.</p><p><b>RESULTS</b>Among the 45 families, 31 SRY-positive fetuses were identified, among whom six were diagnosed with DMD. For 14 SRY-negative fetuses, four were diagnosed as carriers. The remainders were normal.</p><p><b>CONCLUSION</b>MLPA can detect mutations in the exons of dystrophin gene, whilst STR linkage analysis can determine whether the fetus has inherited the maternal X chromosome bearing the mutant gene. As the result, the method can detect affected fetuses in which no exonic mutations are detected with MLPA. By combining the two methods, the diagnostic rate for DMD can be greatly improved.</p>


Subject(s)
Dystrophin , Genetics , Exons , Female , Genetic Linkage , Heterozygote , Humans , Male , Microsatellite Repeats , Multiplex Polymerase Chain Reaction , Muscular Dystrophy, Duchenne , Diagnosis , Genetics , Mutation , Pregnancy , Prenatal Diagnosis
19.
Chinese Journal of Epidemiology ; (12): 677-681, 2013.
Article in Chinese | WPRIM | ID: wpr-318322

ABSTRACT

Objective To find the association between factors related to contraception,reproductive health and the risk of induced abortion among floating married women of childbearing age,so as to provide basis for improving the access to health services for floating women of childbearing age.Methods Using data from the reproductive health survey on floating population from five cities in 2005,factors as demographic characteristics,contraceptive choice,settings and access to health services,induced abortion among the floating married women of childbearing age were described.Multivariate logistic regression was used to investigate the association the factors relative to contraception,reproductive health and the risk of induced abortion between 543 cases and 1796 controls.Results The risks of induced abortion among those under 30-years-old floating married women of childbearing age were 2.08-fold (95%CI:1.26-3.42) of the group at the age of 40 years old.The risk of abortion among floating married women at childbearing age who were taking short-acting contraceptive methods,was 2.56-fold (95% CI:1.84-3.56) of those using the long-acting methods of contraception.The induced abortion risk of floating women at childbearing age who paid the contraceptive implement out of their own pockets,was 1.72-fold (95% CI:1.32-2.24) of those who got it free of charge.The risks of abortion among women who recieved the contraceptive devices through maternal and child health centers,general hospitals or street residential committees were 2.69-fold (95%CI:1.71-4.22),2.49-fold (95%CI:1.68-3.68) and 1.81-fold (95%CI:1.20-2.72) of those who received them from urban or rural family planning stations,respectively.The induced abortion risk for women who were ignorant of emergency contraception,was 1.41-fold (95% CI:1.12-1.78) of those who had the knowledge.The abortion risks of floating women at childbearing age who get the contraceptive knowledge from the colleagues,relatives or friends were 1.85 times (95% CI:1.28-2.67) of those from family planning workers.Conclusion Factors,including age,short-acting contraceptive methods,paid access to contraceptive implement,ignorance of emergency contraception might largely contribute to the increased risk of induced abortion among floating married women of childbearing age,which called for future attention.

20.
Chinese Journal of Epidemiology ; (12): 1238-1242, 2012.
Article in Chinese | WPRIM | ID: wpr-327714

ABSTRACT

Objective To understand the status of abortion and risk factors among floating women of childbearing age and to provide reference for further improvement of induced abortion services in this population.Methods Data on demography,working,and living conditions as well as the use of contraceptive among 4687 persons from a reproductive health survey regarding floating population in five cities in 2005,were involved,while multivariate logistic regression model was used to find out the relationship.Results The risks of abortion among the younger than 30 age group and the 30-39 age groups were 2.21 times (95%CI:1.47-3.34) and 2.38 times (95%CI:1.53-3.70) of the 40-49 age group,respectively.The higher education degree these women had,the higher the risk of abortion was.The risks of abortion among groups having elementary,junior high,senior high school and above,were 2.15 times (95%CI:1.15-4.03),2.47 times (95%CI:1.33-4.57) and 2.61 times (95%CI:1.34-5.11) of those illiterate women.Those having working experience of 2-4 years,5 years or above at the places where the survey was completed,the risks were 2.62 times (95%CI:1.83-3.76)and 7.78 times (95%CI:5.63-10.75) of the less than 2-year-experienced group.The abortion risk of floating women at childbearing age who were living together with their spouses was 1.49 (95%CI:1.05-2.11) times of those women who were not.Conclusion The demographic and lifestyle as well as working features of floating women at childbearing age might increase their risk of abortion.Providing health education regarding these risk factors on the floating women at childbearing age could effectively reduce and prevent the risk of abortion risk among them.

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