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1.
Article in Chinese | WPRIM | ID: wpr-921547

ABSTRACT

Gastrointestinal stromal tumors(GISTs)in the stomach,duodenum,and rectum have low occurrence,and the coexistence GISTs in three parts with neurofibromatosis type Ⅰ(NF-Ⅰ)is even rare.This paper reports a case of GISTs with a family history of NF-Ⅰ.There were multiple nodular masses of different sizes on the patient's face,trunk,and limbs.The patient was admitted due to chest tightness for 5 days and black stools for 1 day.Enhanced CT examination of the abdomen suggested multiple space-occupying lesions in the upper abdomen with multiple small nodules under the abdominal wall,and neurofibromatosis and intestinal stromal tumor cannot be excluded.Finally,surgical pathology confirmed that the multiple tumors in the abdominal cavity were GISTs.The case was confirmed as wild-type GISTs by genetic testing,and the patient recovered well nearly one year after the operation.


Subject(s)
Gastrointestinal Stromal Tumors/genetics , Humans , Neurofibromatosis 1/genetics
2.
Article in Chinese | WPRIM | ID: wpr-887888

ABSTRACT

Objective To understand the current situation and predict the trends in number and composition of prenatal ultrasound screening staff in Beijing. Methods We analyzed the region,age,professional title and other characteristics of prenatal ultrasound screening personnel in Beijing during 2007-2015.We then built an ARIMA model basing on the current situation to predict the number and composition of the staff in 2016-2020. Results The number of prenatal ultrasound screening staff showed an upward trend in 2007-2020 and was predicted to reach 1269 in 2020.During this period,the educational achievement and professional title of the staff showed a downward trend,and the working years became shorter,mainly below 5 years.The proportion of resident doctors remained at 26.6%,and that of the staff receiving further education would reach 43.2% by the end of 2020. Conclusion The prediction under ARIMA model suggests that efforts should be made to strengthen the training of young doctors and provide them opportunities for further study.


Subject(s)
Beijing , Female , Humans , Models, Statistical , Pregnancy , Prenatal Diagnosis , Ultrasonography , Ultrasonography, Prenatal
3.
Article in Chinese | WPRIM | ID: wpr-828443

ABSTRACT

In this study, the clinical characteristics and drug combination rules of Danshen Chuanxiongqin Injection in the treatment of patients with cerebral infarction were analyzed. The inpatient information of 2 857 patients of cerebral infarction treated with Danshen Chuanxiongqin Injection in HIS database of 20 grade Ⅲ class A hospitals in China was collected, and a model was established by description analysis and Apriori algorithm, in order to explore the clinical characteristics and drug combination rules of Danshen Chuan-xiongqin Injection in the treatment of cerebral infarction. The results showed that among patients of cerebral infarction treated with Danshen Chuanxiongqin Injection, 1 727 patients were older than 65 years old, accounting for 69.61%, and 1 610 were males, accounting for 63.59%. Commonly used drugs included lipid-lowering agents, anticoagulant thrombolytic agents, antiplatelet agents, stimulants of brain metabolism, vasodilators and other Western drugs, as well as traditional Chinese medicines, such as blood-activating agents, heat-clearing agents and expectorant agents. The Western medicine with the highest use frequency in combination with Danshen Chuan-xiongqin Injection was aspirin enteric-coated tablets(1 528 cases, 53.48%). The traditional Chinese medicine with the highest use frequency in combination with Danshen Chuanxiongqin Injection was Xingnaojing Injection, with a total of 378 cases, accounting for 13.23%. Among them, the most commonly used Western drugs combined with Danshen Chuanxiongqin Injection were anticoagulant thrombolytic and antiplatelet drugs, with a usage rate as high as 83.48%. In order to further explore the drug combination rules of Danshen Chuanxiongqin Injection, the association analysis of drug combination in patients of cerebral infarction treated with Danshen Chuanxiongqin Injection was carried out. In clinical combination of two Western drugs, Atorvastatin Calcium Capsules+Cerebral Proteolytic Injection were the most common combination, with a support of 27.10%. In clinical combination with 3 Western drugs, Clopidogrel Bisulfate Tablets+Atorvastatin Calcium Capsules+Cerebral Proteolytic Injection were most commonly used, with a support of 15.90%. The results showed that the patients of cerebral infarction treated with Danshen Chuanxiongqin Injection were mainly elderly males, and often complicated with hypertension, coronary heart disease, diabetes and other basic diseases. The clinical application of Danshen Chuanxiongqin Injection was principally in line with the guidelines. In the treatment of cerebral infarction, it was often combined with Western medicine anticoagulant thrombolysis, antiplatelet drugs, traditional Chinese medicine blood-activating and stasis-dissolving prescription and other drugs with similar pharmacological effects, with an auxiliary therapeutic effect on patients of cerebral infarction complicated with other diseases, and can provide guidance for clinical medication.


Subject(s)
Aged , Aspirin , Cerebral Infarction , China , Drugs, Chinese Herbal , Humans , Male , Medicine, Chinese Traditional , Salvia miltiorrhiza
4.
Article in Chinese | WPRIM | ID: wpr-776051

ABSTRACT

Objective To learn the capability and functioning status of prenatal ultrasound screening institutions in Beijing and inform decision-making on the optimization of prenatal screening management. Method By using the data from the second and the third National Special Survey on Prenatal Screening and Prenatal Diagnostic Services in Beijing,we analyzed the distribution,human resources,workload,technology,and equipment of ultrasound screening institutions in Beijing as well as their changing trends. Results From 2013 to 2015,there were 100 prenatal ultrasound screening institutions,833 technicians,and 383 prenatal ultrasound screening instruments in Beijing,offering over 600 000 service sessions.In contrast,there were 93 institutions with a total service volume of 644 100 between 2010 and 2012.The general hospitals,tertiary hospitals,and urban areas(especially urban functional development areas and new urban development areas)were the main sources of the services.In particular,the maternal and child health care hospitals in Beijing provided the highest amount of services per unit of resources.The average prenatal ultrasound screening rate was about 98.05%.The service delivery trended to transfer from urban areas to remote county areas and from secondary institutions to tertiary institutions.Conclusion The operation and management of prenatal ultrasound screening institutions in Beijing are relatively standard.The ultrasound screening rate is relatively high in the second trimester,and the distribution of the services has constantly been improved since 2010.


Subject(s)
Beijing , Female , Hospitals, General , Humans , Mass Screening , Pregnancy , Prenatal Diagnosis , Ultrasonography , Ultrasonography, Prenatal
5.
Article in Chinese | WPRIM | ID: wpr-701155

ABSTRACT

AIM:To investigate the biological function and potential mechanism of leucine-rich repeat kinase 2 (LRRK2)in RAW264.7 macrophages during Mycobacterium tuberculosis infection.METHODS: The bacillus Calmette-Guerin(BCG)-infected RAW264.7 cell model was established.Colony-forming unit(CFU)analysis was used to deter-mine the mycobacterial viability.The releases of interleukin(IL)-1β,IL-6 and interferon-γ(IFN-γ)in the RAW264.7 cells were detected by ELISA.qPCR and Western blot were used to measure the mRNA and protein expression levels,re-spectively.RESULTS:LRRK2 was robustly enhanced in the RAW264.7 cells in response to BCG infection.Additional-ly,silencing of LRRK2 suppressed intracellular growth of mycobacteria during BCG challenge.Moreover, silencing of LRRK2 dramatically attenuated the accumulation of inflammatory cytokines IL-1β,IL-6 and IFN-γinduced by BCG infec-tion.More importantly,LRRK2 modulated BCG-induced inflammatory responses by positively regulating the nuclear factor-κB(NF-κB)signaling pathway.CONCLUSION: LRRK2/NF-κB signaling pathway positively modulates inflammatory responses during BCG infection,which may provide a better understanding of the pathogenesis of tuberculosis and useful in -formation for developing potential therapeutic interventions against the disease.

6.
Chinese Medical Journal ; (24): 510-514, 2015.
Article in English | WPRIM | ID: wpr-357970

ABSTRACT

<p><b>BACKGROUND</b>Non-Hodgkin lymphoma is the fourth most common malignant tumors in children, Burkitt lymphoma (BL) accounts for 30-50% of all pediatric lymphomas. The aim of this study was to investigate the clinicopathologic features, immunophenotype, Epstein-Barr virus (EBV) infection and c-myc gene rearrangement of sporadic BL in children.</p><p><b>METHODS</b>Ninety-two cases of pediatric BL were retrospectively analyzed for clinical features, immunohistochemistry, EBV-encoded RNA (EBER) status by in situ hybridization and c-myc gene rearrangement by fluorescence in situ hybridization.</p><p><b>RESULTS</b>In the 92 cases, male is predominant in sex distribution (M: F = 3.38:1). The average age at diagnosis was 4.97 years. Polypoid BL showed a lower clinical stage (P = 0.002), and advanced clinical stage and low serum albumin level at diagnosis were associated with poor outcome (P = 0.024 and 0.053, respectively). The positive expression of CDl0, B-cell lymphoma-6, MUMl and EBER were 95.7% (88 cases), 92.4% (85 cases), 22.8% (21 cases), 41.3% (38 cases), respectively. The expression of MUM1 were not associated with EBV infection status (P = 1.000). c-myc gene rearrangement was detected in 94.6% (87/92). Clinical treatment information for 54 cases was collected, 21 patients died of tumor after surgery alone, 33 patients received surgery and chemotherapy, and of which six patients died shortly afterwords (MUM1 positive expression in 3 cases, P = 0.076).</p><p><b>CONCLUSIONS</b>The anatomical location, growth pattern and serum albumin level of BL were associated with biological behavior. MUM1 may be a potential adverse prognostic marker, and not associated with EBV infection status.</p>


Subject(s)
Adolescent , Burkitt Lymphoma , Diagnosis , Epidemiology , Metabolism , Child , Child, Preschool , Epstein-Barr Virus Infections , Diagnosis , Metabolism , Female , Humans , Immunohistochemistry , In Situ Hybridization, Fluorescence , Infant , Interferon Regulatory Factors , Metabolism , Male , Sex Distribution
7.
Article in Chinese | WPRIM | ID: wpr-346434

ABSTRACT

The purpose of this study was to prepare tetramethylpyrazine hydrochloride transdermal gel and to study its permeation ability in vitro. The skin permeation ability was evaluated by Valian-Chien permeation cells with isolated rat skin. The concentration of tetramethylpyrazine in samples was determined by HPLC. The optimal formulation was composed with 5% azone, 5% peppermint oil, 8% sodium carboxymethylcellulose and 8% tetramethylpyrazine hydrochloride. The accumulative permeation amount of the gel was (6 731.87 +/- 102.31) microg x cm(-2) in 12 h,and the permeation rate was (535.02 +/- 33.89) microg x cm(-2) x h(-1). The release profile in vitro was in line with zero-order formulation. Tetramethylpyrazine hydrochloride gel prepared in the study would be developed as a novel transdermal preparation.


Subject(s)
Administration, Cutaneous , Animals , Chemistry, Pharmaceutical , Gels , In Vitro Techniques , Male , Permeability , Pyrazines , Chemistry , Pharmacokinetics , Rats , Rats, Sprague-Dawley
8.
Chinese Pharmaceutical Journal ; (24): 1750-1753, 2012.
Article in Chinese | WPRIM | ID: wpr-860584

ABSTRACT

OBJECTIVE: To explore the relationship between flow rate and drug concentration and the recoveries of microdialysis probes. METHODS: Tetramethylpyrazine (TMP) was selected as the model drug. The in vitro recovery of TMP was studied using positive dialysis and retrodialysis methods at different flow rates and concentrations. And the in vivo recovery of TMP was studied using retrodialysis method. RESULTS: The microdialysis recoveries of TMP were inversely proportional to the perfusing rates while independent of TMP concentrations. The recoveries of positive dialysis were quite different from that of retrodialysis in vitro. While the recovery of in vitro positive dialysis was similar to that of in vivo retrodialysis. CONCLUSION: Microdialysis is a useful and reliable tool to study the pharmacokinetics of TMP and in vivo retrodialysis method can be used for TMP pharmacokinetics.

9.
Article in Chinese | WPRIM | ID: wpr-235087

ABSTRACT

<p><b>OBJECTIVE</b>To clone the glyceraldehydes 3-phosphate dehydrogenase (GAPDH) gene of Porphyromonas gingivalis (P. gingivalis) and to induce its fusion expression in E. coli.</p><p><b>METHODS</b>GAPDH was obtained by PCR and was inserted into cloning vector pMD-18-T to construct clone recon. Double enzymes digest the recon pMD18-T-GAPDH and the prokaryotic expression vector pET-32a and then connect to get the expressing recon pET-32a-GAPDH. The recombinant expression plasmid which had been confirmed by enzymes digestion was transformed to E. coli competent cells BL21 and induced the expression of GAPDH with isopropyl beta-D-1-thiogalactopyranoside (IPTG) of different density.</p><p><b>RESULTS</b>DNA sequencing showed that the fragment was 99.802% the same to the sequence published in NCBI. Under the best density, IPTG could be highly expressed.</p><p><b>CONCLUSION</b>The GAPDH had been successfully cloned and expressed in E. coli which gets ready for the following experiment to study the immunity of GAPDH and the homologues antibody preparation.</p>


Subject(s)
Cells, Cultured , Cloning, Molecular , Cloning, Organism , Escherichia coli , Genetic Vectors , Glyceraldehyde , Oxidoreductases , Phosphates , Polymerase Chain Reaction , Porphyromonas gingivalis
10.
Chinese Journal of Pathology ; (12): 819-824, 2010.
Article in Chinese | WPRIM | ID: wpr-295134

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the molecular genetic features and diagnostic aspects of sporadic Burkitt's lymphoma (BL) in children.</p><p><b>METHODS</b>Tissue microarray was constructed to include 64 cases of pediatric BL and 6 cases of pediatric diffuse large B-cell lymphoma (DLBCL). Immunohistochemistry and fluorescence in-situ hybridization for c-myc, bcl-2, bcl-6, IgH, myc/IgH and bcl-2/IgH gene were performed. Cases of pediatric Burkitt's lymphomas were subclassified into three groups based on their cellular orgins: the germinal center (GC) group, the late-germinal center (late-GC) group and the post-germinal center (post-GC) group.</p><p><b>RESULTS</b>Among 64 Burkitt's lymphomas studied, expression of CD20, CD10, bcl-6, bcl-2 and MUM1 by immunohistochemistry were 100% (64 cases), 98.4% (63 cases), 96.9% (62 cases), 0 (0 cases) and 23.4% (15 cases), respectively. Various gene rearrangements were found involving the c-myc 93.1% (54/58 cases) and IgH 82.8% (48/58 cases). Detailed rearrangements are as follows: 46 cases (85.2%) myc/IgH gene translocation along with c-myc and IgH gene rearrangement; 4 cases (7.4%) c-myc gene rearrangement without IgH and myc/IgH abnormality; 4 cases (7.4%) without c-myc, IgH or myc/IgH gene rearrangement. No case showed bcl-2 gene abnormality (100%). Fifty nine cases showed normal bcl-6 gene status. One case had bcl-6 gene rearrangement and amplification with the pathologic and immunophenotypic characteristics of BL, leading to a revised pathological diagnosis of B-cell lymphoma, unclassifiable with features intermediate between diffuse large B-cell lymphoma and Burkitt's lymphoma (DLBCL/BL). Two cases showed c-myc gene rearrangement. Two cases showed bcl-6 gene amplification and 6 DLBCL cases had a normal status of bcl-2/IgH.</p><p><b>CONCLUSIONS</b>A majority of pediatric sporadic BL arise from the germinal center B cells, most of which have c-myc gene rearrangement. It is useful to distinguish BL and DLBCL by multiple genes detection.</p>


Subject(s)
Antigens, CD20 , Metabolism , Burkitt Lymphoma , Genetics , Metabolism , Pathology , Child , Child, Preschool , Diagnosis, Differential , Female , Gene Rearrangement, B-Lymphocyte, Heavy Chain , Genes, myc , Genetics , Humans , Immunoglobulin Heavy Chains , Genetics , Lymphoma, Large B-Cell, Diffuse , Genetics , Metabolism , Pathology , Male , Neprilysin , Metabolism , Proto-Oncogene Proteins c-bcl-2 , Metabolism , Proto-Oncogene Proteins c-bcl-6 , Metabolism , Translocation, Genetic
11.
Chinese Journal of Pathology ; (12): 759-764, 2009.
Article in Chinese | WPRIM | ID: wpr-249047

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinicopathologic features, immunohistochemical findings, EBV and c-myc gene status of intra-abdominal non-Hodgkin B-cell lymphoma occurring in children.</p><p><b>METHODS</b>Seventy-four cases of pediatric intra-abdominal non-Hodgkin B-cell lymphoma were retrieved from the archival file. The cases were classified according to the 2008 WHO classification. Tissue microarray including tumor tissues from all the 74 cases was produced. Immunohistochemical study (SP method) for CD20, CD3, CD79a, CD10, bcl-6, MUM1, bcl-2, CD43, CD38 and Ki-67 was performed. In-situ hybridization for Epstein-Barr virus-encoded RNA (EBER) and fluorescence in-situ hybridization for c-myc gene were also carried out.</p><p><b>RESULTS</b>Amongst the 74 cases studied, 65 of them (87.8%) were Burkitt lymphoma (BL), 4 cases (5.4%) were diffuse large B-cell lymphoma (DLBCL) and the remaining 5 cases (6.8%) showed features in-between DLBCL and BL (DLBCL/BL). The patients often presented with abdominal pain, abdominal masses, ileus and intussusception. The ileocecal bowel wall and mesenteric lymph nodes were commonly involved. The lymphoma cells were of high histologic grade and suggested an aggressive clinical behavior. The staining for CD20 and CD79a were positive in all of the cases, while CD3 was negative. The positive rates of CD10, bcl-6, bcl-2, MUM1, CD43, CD38 and EBER in BL were 96.9% (63 cases), 95.4% (62 cases), 0 (0 case), 23.1% (15 cases), 70.8% (46 cases), 96.9% (63 cases) and 41.5% (27 cases), respectively. Fifty-four cases carried translocation of c-myc gene. As for DLBCL, the positive cases of CD10, bcl-6, bcl-2, MUM1, CD43, CD38 and EBER were 3 cases, 2 cases, 3 cases, 2 cases, 2 cases, 2 cases and 0 case, respectively. One of these cases showed c-myc gene translocation. Amongst the 4 cases of DLBCL, 2 of them belonged to germinal center B-cell-like subtype, while the remaining 2 cases were of non-germinal center B-cell-like subtype. The expression rates of CD10, bcl-6, bcl-2, MUM1, CD43, CD38 and EBER in DLBCL/BL were 5/5, 4/5, 0, 3/5, 5/5, 3/5 and 0, respectively. Three of the cases were positive for c-myc gene translocation.</p><p><b>CONCLUSIONS</b>The majority of pediatric intra-abdominal non-Hodgkin B-cell lymphoma belonged to BL. They have characteristic clinical presentation and sites of predilection and are often associated with an aggressive clinical behavior. Thorough morphologic assessment, immunohistochemistry and in-situ hybridization play an important role in subtyping this group of lymphoid malignancy.</p>


Subject(s)
Antigens, CD20 , Metabolism , Burkitt Lymphoma , Genetics , Metabolism , Pathology , CD79 Antigens , Metabolism , Child , Child, Preschool , Female , Genes, myc , Humans , Intestinal Neoplasms , Genetics , Metabolism , Pathology , Lymphoma, B-Cell , Genetics , Metabolism , Pathology , Lymphoma, Large B-Cell, Diffuse , Genetics , Metabolism , Pathology , Male , Neprilysin , Metabolism , RNA, Viral , Metabolism , Translocation, Genetic
12.
Article in Chinese | WPRIM | ID: wpr-297011

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect of electroacupuncture (EA) with different stimulation parameters on medicine-induced abortion.</p><p><b>METHODS</b>One hundred and nine cases of early pregnancy who asked medicine-induced abortion were allocated to an EA group A (n = 37), an EA group B (n = 38) and a medication group (n = 34). Within 30-60 min after oral administration of Misoprostol, in the EA group A, EA was given at bilateral Hegu (LI 4) and Sanyinjiao (SP 6) with cluster waves of 100 Hz and in the EA group B, EA was given at Hegu (LI 4) for 20 min and then at Sanyinjiao (SP 6) for 5 min with continuous waves of 50 Hz. EA was not given to the medication group. The complete abortion rate, duration of eliminating embryonic sac, colporrhagia lasting time and abdominal pain condition were recorded.</p><p><b>RESULTS</b>The complete abortion rate was 91.9% in the EA group A and 86.8% in the EA group B, which were higher than 82.4% in the medication group, with no significant differences between the 3 groups (P>0.05); the duration of eliminating embryonic sac and the colporrhagia lasting time in the two EA groups were significantly shorter than those in the medication group (P<0.05, P<0.01); alleviation of abdominal pain in the EA group B was better than the medication group (P<0.01) and the EA group A (P<0.05).</p><p><b>CONCLUSION</b>Different stimulation parameters of EA have different effects on abortion.</p>


Subject(s)
Abortion, Induced , Methods , Adult , Electroacupuncture , Female , Humans , Pregnancy
13.
Article in Chinese | WPRIM | ID: wpr-348403

ABSTRACT

<p><b>OBJECTIVE</b>To search for a therapeutic method for alleviating the short-term adverse effect of drug-induced abortion.</p><p><b>METHODS</b>Ninety cases of early pregnancy were divided into 3 groups randomly. Control group were treated with medicine, electroacupuncture group I with electroacupuncture at both Hegu (LI 4) and Sanyinjiao (SP 6) simultaneously within 30-60 min after the medicine was given, and electroacupuncture group II with electroacupuncture at Hegu (LI 4) and Sanyinjiao (SP 6) successively within 30-60 min after the medicine was administrated.</p><p><b>RESULTS</b>After treatment, the short-term adverse effect in the electroacupuncture groups was more significantly alleviated as compared with the control group (P < 0.05), and alleviative degree of abdominal pain in the electroacupuncture group II was better than that in the electroacupuncture group I .</p><p><b>CONCLUSION</b>Electroacupuncture at Hegu (LI 4) and Sanyinjiao (SP 6) can alleviate short-term adverse effects of drug-induced abortion, and first electroacupuncture at Hegu (LI 4) followed by Sanyinjiao (SP 6) can more significantly alleviate abdominal pain.</p>


Subject(s)
Abdominal Pain , Therapeutics , Abortion, Induced , Acupuncture Points , Adult , Electroacupuncture , Methods , Female , Humans , Pregnancy
14.
Article in Chinese | WPRIM | ID: wpr-235223

ABSTRACT

<p><b>OBJECTIVE</b>Taking Chinese compound medicine Yuchangning as a research model, prepared the pH and time dependent Yuchangning tablets for colon-specific delivery (PT-YT-CSD), and evaluated the releasing property in vitro.</p><p><b>METHOD</b>The coating prescription is filtered by the release extent of matrine and oxymatrine in vitro and the wicking rate of the tablet, which including the category and proportion of film forming agent and porogen, the sort and dosage of fluidizing agent, the increment of weight after coating and so on. The releasing property of the preparation is evaluated by the dissolution tests in vitro through measuring the content of matrine and oxymatrine content.</p><p><b>RESULT</b>The preparation method of the PYTCSD: After prepared plain tablets, the 95% alcoholic solution of EC and Eudragit II are mixed in a 7:3 EC: Eudragit II ratio and then added in DEP up to 10% of the coating amount, reduced the alcohol concentration to 4% by diluting with ethonal. Tablet was coated by the alcohol solution and the weight of the plain tablet was increased by 3%. The dissolution tests in vitro indicated that matrine and oxymatrine were not dissolved in the simulated gastric juice after 2 h. The accumulative quantities of matrine and oxymatrine were less than 10% in the simulated intestinal juice after 4 h. The quantities of matrine and oxymatrine are 75.7% and 76.8% in the simulated colon juice after 1 h.</p><p><b>CONCLUSION</b>The PYTCSD was prepared and the preparation could fulfil the aim of delivering in the specific colon in vitro.</p>


Subject(s)
Alkaloids , Astragalus propinquus , Chemistry , Cellulose , Colon , Metabolism , Delayed-Action Preparations , Drug Combinations , Drug Compounding , Drug Delivery Systems , Drugs, Chinese Herbal , Pharmacokinetics , Hydrogen-Ion Concentration , Intestinal Absorption , Plants, Medicinal , Chemistry , Polymethacrylic Acids , Quinolizines , Sophora , Chemistry , Tablets, Enteric-Coated
15.
Article in Chinese | WPRIM | ID: wpr-246053

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect of Fuzheng Jiangan formula( FZJGF) on liver fibrosis using immune induced liver fibrosis rat model.</p><p><b>METHOD</b>The rat models with immunity liver fibrosis were induced by the human serum albumin. Rats were treated with normal saline, FZJGF (9. 85,39. 4 g x kg(-1) , two dosage groups) and Colchicine (0. 000 1 g . kg(-1) ). The activities of ALT, AST, contents of ALB and TP, and A/G, The contents of Laminin (LN), Hyaluronic acid (HA) and collagen type IV (IV-C) in rat serum were measured by radioimmunoassay method. The level of hydroxyproline (Hyp) in liver was detected by chemistry method. The pathological changes of liver tissue were observed by HE and Von-Gieson staining.</p><p><b>RESULT</b>FZJGF could significant decrease the serum activities of ALT and AST, and increase the levels of TP,Alb and ratio A/G. The levels of LN, HA and IV-C were decreased significantly after the treatment using FZJGF. The pathological improvements were observed. FZJGF could markedly alleviate the deposition of collageneous fiber, and reduce the liver pseudoluboli and the fibrosis scores in the liver tissue compared with model group.</p><p><b>CONCLUSION</b>FZJGF can inhibit formation and development of rat hepatic fibrosis induced by the human serum albumin.</p>


Subject(s)
Alanine Transaminase , Blood , Animals , Aspartate Aminotransferases , Blood , Collagen Type IV , Blood , Drugs, Chinese Herbal , Pharmacology , Humans , Hyaluronic Acid , Blood , Hydroxyproline , Metabolism , Laminin , Blood , Liver , Metabolism , Pathology , Liver Cirrhosis, Experimental , Blood , Male , Plants, Medicinal , Chemistry , Random Allocation , Rats , Rats, Wistar , Serum Albumin
16.
Article in Chinese | WPRIM | ID: wpr-255316

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect of recombinant human epithelial growth factor (rhEGF) in promoting the healing of cervical erosion.</p><p><b>METHODS</b>Forty-eight patients with cervical erosion were treated with rhEGF and 30 with 500 kHz high-frequency electromagnetic wave, and the effects of the therapies were compared in terms of healing of the cervical wound, healing time, volume of vaginal discharge and bleeding and the lasting time.</p><p><b>RESULTS</b>In comparison with radiofrequency therapy, the healing of the lesion took significantly shorter time with rhEGF therapy, which also resulted in less vaginal discharge that lasted for shorter time without causing vaginal bleeding.</p><p><b>CONCLUSION</b>rhEGF can obviously accelerate the healing of cervical erosion.</p>


Subject(s)
Adult , Electromagnetic Phenomena , Epidermal Growth Factor , Genetics , Therapeutic Uses , Female , Humans , Middle Aged , Recombinant Proteins , Therapeutic Uses , Treatment Outcome , Uterine Cervical Erosion , Drug Therapy , Pathology , Therapeutics , Wound Healing
17.
Acta Pharmaceutica Sinica ; (12): 619-624, 2006.
Article in Chinese | WPRIM | ID: wpr-294971

ABSTRACT

<p><b>AIM</b>To investigate the effect of baicalin on the hippocampal neuronal apoptosis and the expression of HSP70 in rats with focal brain ischemia-reperfusion injury.</p><p><b>METHODS</b>One hundred and twenty male Wistar rats were randomly divided into six groups:sham operated group, ischemia-reperfusion group, nimodipine group and three baicalin groups,to which baicalin was administered at doses of 50, 100 and 200 mg x kg(-1), separately. The models of focal brain ischemia-reperfusion injury induced by middle cerebral artery occlusion (MCAO) were used in this study. HE stain was used to observe the pathological changes. Flow cytometry (FCM) was used for determination of neuronal apoptosis. HSP70 protein expression of the neurons was detected with immunohistochemistry. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of the mRNA level of HSP70.</p><p><b>RESULTS</b>Baicalin can significantly relieve the pathological changes and inhibit apoptosis in hippocampus CA1 area, and at the same time increase the expression of HSP70 and HSP70 mRNA.</p><p><b>CONCLUSION</b>Baicalin can relieve brain damage induced by focal brain ischemia-reperfusion in rats, which may be related to inhibiting the process of the neuronal apoptosis. The mechanism of antiapoptosis effect of baicalin may be related to the promotion of transcription of HSP70 mRNA and increasing the expression of the protein.</p>


Subject(s)
Animals , Apoptosis , Flavonoids , Pharmacology , Flow Cytometry , HSP70 Heat-Shock Proteins , Genetics , Hippocampus , Metabolism , Pathology , Infarction, Middle Cerebral Artery , Male , Neurons , Metabolism , Pathology , Neuroprotective Agents , Pharmacology , Plants, Medicinal , Chemistry , Pyramidal Cells , Metabolism , Pathology , RNA, Messenger , Genetics , Random Allocation , Rats , Rats, Wistar , Reperfusion Injury , Genetics , Metabolism , Reverse Transcriptase Polymerase Chain Reaction , Scutellaria , Chemistry
18.
Article in Chinese | WPRIM | ID: wpr-287081

ABSTRACT

<p><b>AIM</b>To investigate the changes of enkephalin mRNA and prodynorphin mRNA gene expression in rat brain regions with chronic immobilization stress and the influence of Chinese herbs.</p><p><b>METHODS</b>We copied the rat model of chronic immobilization stress (3 h daily , repeated 7 d or 21 d), and primers of enkephalin or prodynorphin were respectively added for RT-PCR reaction (BETA-actin as inner contrast). Gel image analysis system was used to scan and analyze and odds of optical density of target gene and inner contrast strip were taken as quasi-quantified data.</p><p><b>RESULTS</b>Prodynorphin mRNA expression in hippocampus markedly increased in 7 d group (P < 0.01), while enkephalin mRNA prodynorphin mRNA expression in hippocampus markedly increased in 21 d group (P < 0.01). All three recipes were able to decrease the gene expression of prodynorphin mRNA (P < 0.01), and xiaoyao powder as well as sijunzi soup were able to decrease the gene expression of enkephalin mRNA (P < 0.01).</p><p><b>CONCLUSION</b>The effects of xiaoyao powder on the above two gene expression was better than jinkuishenqi pill.</p>


Subject(s)
Animals , Drugs, Chinese Herbal , Pharmacology , Dynorphins , Metabolism , Enkephalins , Metabolism , Gene Expression , Hippocampus , Metabolism , Male , RNA, Messenger , Genetics , Rats , Rats, Sprague-Dawley , Restraint, Physical , Stress, Physiological
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