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1.
Article in Chinese | WPRIM | ID: wpr-866042

ABSTRACT

Objective:To develop a learning scale of scientific methods for medical students and to evaluate the reliability and validity of the scale.Methods:Based on the principles of planned behavior theory, the original scale was developed through literature analysis, pre-interview and experts’ consultation. A preliminary survey was conducted among 105 medical undergraduates with random cluster sampling, and the primary scale was formed by exploratory factor analysis. Then, 851 undergraduates were selected as the objects of formal survey, and the formal scale was finally determined by the reliability and validity evaluation and confirmatory factor analysis.Results:The formal scale was composed of 5 latent variables, 9 observed variables and 51 items. The overall Cronbach’s α coefficient and the overall retest reliability were 0.970 and 0.887, respectively. Composite reliability and KMO values of each latent variable were more than 0.8, and the values of average variance extraction were over 0.5. The fitness test ( P=0.155) showed acceptable fitting quality with the main fit indices of χ2/ df=1.405, RMR=0.039, RMSEA=0.022, etc. Conclusion:The learning scale of scientific methods shows satisfactory reliability, validity and fitness, which can be applied as a measuring tool to evaluate the learning behavior of scientific methods for medical students.

2.
Article in Chinese | WPRIM | ID: wpr-692851

ABSTRACT

Objective An updated Meta-analysis was undertaken to evaluate the recent progress in the as-sociation of lipoprotein lipase (LPL) gene HindⅢ polymorphism with diabetes mellitus (DM).Methods Da-tabases including PubMed ,OvidSP ,Web of Science ,CBM ,CNKI and Wanfang were systemically searched to collect all case-control studies on the correlation between LPL HindⅢ polymorphism and DM.According to the selection criteria ,2 reviewers independently screened the literatures ,extracted data and evaluated the quali-ty of the included studies.Meta-analysis was completed by using Stata 14.0 software.Results 21 groups of data from 20 literatures were included with 2 634 DM patients and 3 375 controls.The results of meta-analysis showed that the frequencies of G allele (OR=0.83 ,95% CI :0.69 -0.99 ,P=0.04) and the dominant model [(TG+GG) vs.TT :OR=0.79 ,95% CI :0.63-0.99 ,P=0.04] in DM patients were associated with the re-duction in the risk of DM.Compared with TT genotype ,plasma triglyceride levels were obviously reduced in DM patients with TG +GG genotype and GG genotype.Furthermore ,total cholesterol levels in DM patients with TG+GG genotype were markedly decreased ,while HDL-C levels were significantly increased in DM pa-tients with TG+ GG genotype.Conclusion The G allele and TG + GG genotype of LPL HindⅢ polymor-phism may be protective factors for DM ,which can reduce the risk of DM by ameliorating the abnormalities of lipid metabolism.However ,large-scale and high-quality clinical trials on the association between LPL HindⅢ polymorphism and DM are still needed for further clarification.

3.
Article in Chinese | WPRIM | ID: wpr-692205

ABSTRACT

OBJECTIVE To explore the effect of the pip fixation of the chest on air leakage through continuous positive airway pressure (CPAP) treatment. METHODS 106 patients with obstructive sleep apnea hypopnea syndrome were enrolled for this study. All patients underwent a whole night automatic titration for CPAP treatment. The patients included were divided into two groups randomly. For one group, the pipes were fixed on the chest (fixed group), while for the other group, there were no additional fixations for the pipes (control group). The data of all patients, including age, body mass index, AHI, and results of the titrations, were all collected for analyses. RESULTS Among the patients included, 42 patients belonged to the fixed group (39. 6%), while 64 patients belonged to control group (60. 4%). The average oxygen saturation of the Pipe-fixed group was significantly higher than that of the control group, while there were no significant differences for other parameters between the groups. However, the air leakage volume of the fixed group, (26. 5±7. 3)L/min,was significantly lower than that of the control group, (29. 9±9. 1)L/min (P=0. 041). The linear regression analyses suggested that the average pressure(coefficient=2. 124,P<0. 001) and the fixation of the pipe (coefficient=-2. 541, P=0. 050) had predictive value for the air leakage volume (P<0. 05). The equation was: the air leakage volume=13. 093+2. 124xthe average pressure -2. 541 × the fixation of the pipe (the data is 2 for the fixed group and 1 for the control group), the R square and the adjusted R square values of the analyses were 0. 398 and 0. 386. CONCLUSION The treatment pressure is positively related to the air leakage volume in CPAP treatment, a higher pressure suggested a higher potential air leakage volume, while the fixation of the pipe could be helpful in reducing the air leakage volume.

4.
Article in Chinese | WPRIM | ID: wpr-613161

ABSTRACT

OBJECTIVE To evaluate the value of Epworth sleepiness scale(ESS) combined with home-based portable monitoring(PM) in the diagnosis of patients with obstructive sleep apnea syndrome(OSAS). METHODS Adult OSAS patients who visited Beijing Tongren Hospital and complained of snoring were included in this study. The overnight polysomnography(PSG) and the ESS were completed at the sleep center and the portable multi-channel monitoring was completed within one week. Subjects were divided into two groups according to their apnea hypopnea index(AHI) of PSG: simple snoring or mild OSAS group(AHI<15/h) and moderate-severe group(AHI≥15/h). The ESS score, respiratory disturbance index(RDI) of PM and other parameters were compared and the correlation of AHI with ESS scores, RDI were analyzed. The sensitivity and specificity of ESS score≥9 points, RDI≥15/h in the diagnosis of moderate-severe OSAS were calculated. RESULTS A total of 51 subjects were included in this study with 42 males(82.4%) and 9 females(17.6%). Mean age was(43.8±10.8) years, and the mean body mass index was(27.9±4.5)kg/m2; ESS scored 1-24 points and mean(8.6±5.0) points, 18 cases with ESS score ≥9 points; AHI ranged between 2.5 and 99.8/h with a mean of(37.4±29.8)/h. There were 19(37.3%) and 32(62.7%) patients in snoring-mild group and moderate-severe group, respectively. There was significant difference for ESS scores between the two groups(9.9±5.1 vs 6.6±4.2 points, P<0.05),RDI in moderate-severe OSAS group was significantly higher than that in snoring-mild group[(49.4±23.1)/h vs (6.8±4.5)/h, P<0.001)]. There was a positive correlation between the ESS score and PSG derived AHI(r =0.435, P =0.002); the PM derived RDI were significantly correlated with PSG derived AHI in snoring-mild group(r =0.706, P=0.001), moderate-severe group(r =0.873, P=0.000) and patients with ESS score ≥9 points(r =0.967, P=0.000). At a PSG threshold AHI=15/h, the sensitivity and specificity for ESS score ≥9 points were 45.2% and 78.9%; the PM derived RDI≥15/h had a sensitivity and specificity of 84.4% and 84.2%, respectively; the sensitivity and specificity for each individual screening tool along with the combination of ESS and PM were 100.0% and 100.0%. CONCLUSION ESS combined with PM diagnostic method is a valuable measure for the diagnosis of moderate and severe OSAS patients.

5.
China Pharmacy ; (12): 3275-3277, 2015.
Article in Chinese | WPRIM | ID: wpr-500989

ABSTRACT

OBJECTIVE:To observe clinical efficacy and safety of mosapride combined with flupentixol melitracen in the treat-ment of elderly functional heartburn. METHODS:106 elderly patients with functional heartburn in accordance with Rome IIIstan-dard were randomly divided into control group(52 cases)and treatment group(54 cases). The control group was treated with Mo-sapride dispersible tablets 5mg,tid,orally;the treatment group was additionally given Flupentixol melitracen tablets(containing flu-pentixol 0.5 mg and melitracen 10 mg),qd,on the basis of control group. Therapeutic efficacy of 2 groups were observed after 4 weeks. Hamilton anxiety scale(HAMA),Hamilton depressive scale(HAMD) and symptom score were conducted before and after treatment. RESULTS:The total effective rate of treatment group and control group were 87.0%and 53.8%;with statistical signifi-cance(P<0.05);there was statistical significance in symptom score,HAMA score and HAMD score between 2 groups after treatment (P<0.01). No severe ADR was found in 2 groups. CONCLUSIONS:Mosapride combined with flupentixol melitracen is safe and ef-fective in the treatment of elderly functional heartburn.

6.
Article in Chinese | WPRIM | ID: wpr-233787

ABSTRACT

<p><b>OBJECTIVE</b>To explore whether there were differences between the results of automatic titration and the results of manual titration for positive airway pressure treatment in patients with obstructive sleep apnea hypopnea syndrome (OSAHS) and its influencing factors, the results might provide a theoretical basis for the rational use of two pressure titration methods.</p><p><b>METHODS</b>Sixty one patients with OSAHS were included in this study. All patients underwent a manual titration and an automatic titration within one week. The clinical informations, polysomnography data, and the results of both two titration of all patients were obtained for analysis.</p><p><b>RESULTS</b>The overall apnea/hypopnea index was (63.1 ± 17.7)/h, with a range of 14.9/h to 110.4/h. The treatment pressure of manual titration was (8.4 ± 2.1) cmH(2)O, which was significantly lower than the treatment pressure of automatic titration, (11.5 ± 2.7) cmH(2)O (t = -9.797, P < 0.001). After using a ΔP of 3 cmH(2)O for the cutoff value (ΔP was defined as the difference of automatic titration and manual titration), it was found that the pressure of automatic titration was significantly higher in patients with a ΔP > 3 cmH(2)O than in patients with a ΔP ≤ 3 cmH(2)O, which was (13.3 ± 2.3) cmH(2)O vs (10.0 ± 2.0) cmH(2)O (t = -6.159, P < 0.001). However, there were no differences for the pressure of manual titration between these two groups, which was (8.6 ± 2.4) cmH(2)O vs (8.3 ± 2.0)cmH(2)O (P > 0.05). There was no significant difference in age, body mass index, neck circumference, abdomen circumference, apnea hypopnea index, and arterial oxygen saturation between these two groups.</p><p><b>CONCLUSIONS</b>The treatment pressure of automatic titration is usually higher than that of manual titration. For patients with a high treatment pressure which is derived from automatic titration, a suggestion about manual titration could be given to decrease the potential treatment pressure of continuous positive airway pressure, which may be helpful in improving the comfortableness and the compliance of this treatment.</p>


Subject(s)
Body Mass Index , Continuous Positive Airway Pressure , Methods , Humans , Oximetry , Patient Compliance , Polysomnography , Pressure , Sleep Apnea, Obstructive , Therapeutics
7.
Article in Chinese | WPRIM | ID: wpr-430642

ABSTRACT

Objective To summarize the experiences in the diagnosis and treatment of the hepatic hereditary hemorrhagic telangiectasia (HHHT).Methods The clinical data of 15 HHHT patients who were admitted to the Qilu Hospital,People's Hospital of Mengyin,People's Hospital of Liaocheng,Henan Provincial People's Hospital,the Second Hospital of Hebei Medical University,First Affiliated Hospital of Zhejiang University were retrospectively analyzed.The clinical manifestation,features of imaging and laboratory examination were summarized,and the diagnosis,treatment and prognosis of the disease were investigated.Results HHHT patients had nonspecific symptoms in the early stage,and some patients presented with right upper quadrant discomfort,shortness of breath,anemia and liver bruit.The condition of HHHT patients could be worsened by liver cirrhosis or portal hypertension rapidly.The results of color doppler ultrasound and computed tomography showed intrahepatic telangiectasia,arteriovenous fistula and hepatic artery aneurysm in the 15 patients.Digital subtraction angiography was not clear enough for 2 HHHT patients with more than 1 enlarged hepatic arteries,but computed tomographic angiography was feasible.According to the degree and stages of the HHHT,all the 15 patients were divided into asymptomatic HHHT,simple HHHT and complex HHHT.Among the 6 patients who underwent surgical treatment,5 received ligation or banding of the enlarged hepatic arteries with subsequent disappearance of symptoms.Three patients received interventional treatment,and the treatment for 1 patient with complex HHHT was failed,and the patient died 30 months after medical treatment.Six patients were treated by conservative treatment,2 patients of them had no symptoms at the beginning,then they suffered from hepatic dysfunction and ascites at 21 and 35 months,respectively,and 1 of them died 6 months later.Four patients received medical treatment,and the results of color doppler ultrasound and computed tomography showed the pathological changes were aggravated gradually.Conclusions Telangiectasia,intrahepatic arteriovenous fistula and hepatic artery aneurysm are the main imaging characteristics of HHHT,and imaging diagnosis has significant value in the diagnosis of HHHT.HHHT is a progressive disease,early,active and individualized treatment is beneficial to the patients.The outcome of ligation or banding of the hepatic arteries is satisfactory.

8.
Chinese Journal of Digestion ; (12): 750-753, 2010.
Article in Chinese | WPRIM | ID: wpr-383052

ABSTRACT

Objective To explore the diagnostic value of multi-slice computer tomography (MSCT) in cause and location of agnogenic intestinal obstruction. Methods With light speed 16 multiple slice spiral-CT, 68 patients clinically suggested intestinal obstruction were underwent plain scan and second phase enhanced scan in whole abdomen. The image data was post-processed through GEADW4. 4 station with multi-planar reconstruction (MPR) and maximum intensity projection (MIP). The CT image characters of agnogenic intestinal obstruction were analyzed by comparing CT image suggestions with operation findings. Results MSCT clearly indicate the location, cause and severity of intestinal obstruction, and the diagnose accordance rate with operation findings was 95.6 % (65/68). Conclusions MSCT is a quick, simple and effective means for diagnosis of the location,causes and severity of agnogenic intestinal obstruction. It could provide an important clue for the doctors when making clinical decisions.

9.
Chinese Journal of Nephrology ; (12): 294-298, 2009.
Article in Chinese | WPRIM | ID: wpr-380962

ABSTRACT

Objective To investigate the effects of lipoprotein lipase activator, NO-1886, on the mRNA and protein expression of glycogen synthase kinase-3β (GSK-3β) in the kidney of diet-induced diabetic minipigs. Methods Fifteen Guangxi Bama minipigs were randomized into three groups: C group (n=5, with the normal control diet), DM group (n=5, with the high-fat and high-sucrose diet), and NO-1886 group (n=5, with the high-fat and high-sucrose diet supplemented with 1.0% NO-1886). Plasma glucose, insulin, tfiglyceride (TG), oral glucose tolerant test, creatinine and blood urea nitrogen were measured monthly. Urinary samples in the morning were used for determination of microalbumin at month 0, 2, 4 and 5. The mRNA and protein expression of GSK-3β were measured by real time PCR, Western blot and immunohistochemistry in the kidneys obtained at the end of month 5. Results Compared with the C group, levels of plasma glucose, insulin, triglyceride and mieroalbuminuria were significantly increased in the DM group. The mRNA and protein expression of GSK-3β were increased in the kidneys of diabetic pigs (mRNA 0.0272±0.0052, protein 1.1600±0.0463, P<0.01) as compared with those of normal pigs (mRNA 0.0125±0.0045, protein 0.1385±0.0664). Compared with the DM group, the concentrations of plasma glucose, insulin, triglyceride and mieroalbuminuria obviously decreased in the NO-1886 group. The mRNA and protein expression of GSK-3β were decreased in the kidneys of the NO-1886 group (mRNA 0.0162±0.0019, protein 0.8429±0.0408, P<0.05) as compared with that of the DM group. Conclusion NO-1886 can improve disorders of glucose and TG metabolism and insulin resistance, and down-regulate the expression of GSK-3β in the kidneys, and protect renal function and morphologie damage in diet-induced diabetic minipigs.

10.
Article in Chinese | WPRIM | ID: wpr-545727

ABSTRACT

Objective To establish the method for determination of borax in food by RP-HPLC. Methods Borax in food was extracted by acid methanol followed by turmeric acetic acid derivation and then detected by HPLC. Symmtry C18(150 mm?4.6 mm, 5 ?m) was used as the stationary phase, methanol-water (80+20) as the mobile phase, the detection wavelength was 550 nm. Results The detection limit was 6.4 ng/kg, the relative error was 2.3%, the recovery rates were between 90.63% and 96.88%, the liner range of the borax was 0.032-0.32 ?g/ml, the regressive equation of calibration curve was y=3 210 000x+121 000, r=0.999 2. Conclusion The present method is simple, rapid, accurate with satisfactory results.

11.
Article in Chinese | WPRIM | ID: wpr-564511

ABSTRACT

Objective To investigate the protective the effect of NO-1886 on the expression of protein kinase C in the kidneys of diabetic minipig model induced by high-sucrose and high-fat diet.Methods 15 Guangzhou minipigs aged 3 months were randomly divided into 3 groups of normal control,diabetes,diabbetes treatment,which were fed by basaldiet,high sucrose and high fat feed or with 1.0%No-1886 respectively.These minipigs were killed at the end of 5th month.Minipigs fed with high fat/high sucrose diet were treated with No-1886,and The fasting concentrations of plasma glucose,triglyceride,serum insulin and PKC were observed.Results High fat high sucrose feeding elevated fasting plasma glucose,trglyceride and serum insulin levels significantly.Supplement of No-1886 into high fat high sucrose diet induced a decrease in plasma glucose,triglyceride,insulin and PKC concentration compared with pigs fed with the sole high fat high sucrose diet.Conclusion No-1886 suppressed plasma glucose,triglyceride,insulin and PKC level.

12.
Article in Chinese | WPRIM | ID: wpr-528340

ABSTRACT

OBJECTIVE To investigate the effects of obstructive sleep apnea hypopnea syndrome on the quality of life of the patients. METHODS The quality of life was assessed with the Medical Outcomes Study SF-36 Health Survey in 145 patients with OSAHS and 17 snorers without OSAHS. Excessive Daytime Sleepiness was assessed with Epworth Sleepiness Scale. Pearson linear correlation and stepwise multiple regression analysis were used to study the association between each domain score of SF-36 and patients information,PSG parameters,EDS scores. RESULTS Three domains of SF-36 correlated negatively with Body Mass Index. Four domains correlated negatively with EDS. Three domains and total score correlated significantly with the lowest oxygen saturation. Two domains correlated negatively with the longest time of apnea. AHI and the lowest oxygen saturation have a mild side effect on the quality of life. BMI and EDS score have significantly side effect on the quality of the life. CONCLUSION Comparing to the mild OSAHS patients and snorers without OSAHS,the severe OSAHS patients have worse quality of life. EDS score and BMI have a significantly side effect on the severe OSAHS patients. OSAHS has a side effect on the quality of life of the patients.

13.
Journal of Biomedical Engineering ; (6): 1024-1044, 2005.
Article in Chinese | WPRIM | ID: wpr-238287

ABSTRACT

The relationship between Human papillomavirus (HPV) 16 infection and the natural course of colorectal adenocarcinoma has not been fully defined. In this study, the HPV 16 E7 DNA was detected in 82 patients with primary colorectal adenocarcinoma to study the relationship between HPV 16 infection and colorectal carcinoma. Samples were taken from both the tumors and the adjacent normal mucosa in the same patient. Polymerase chain reaction (PCR) was used to amplify the HPV16 E7 DNA fragment. The PCR products were gel-purified and sequenced for HPV genotyping. DNA sequence analysis indicated that PCR product was 297 bp. It was the equivalent of 562-858th pairs in the HPV 16 primitive sequences. Our results showed HPV16 E7 DNA expression was significantly higher in colorectal carcinoma (42/82) than in adjacent normal mucosa (4/82). The correlation was found between HPV16 E7 expression and tumor's location; the positive rate was 18.18% in the ascending colon carcinoma group and 64.10% in the rectal carcinoma group. High HPV16 E7 expression was also associated with lower Dukes stage (P < 0.01). These results indicated that there was correlation between colorectal adenocarcinoma and HPV 16 infection. HPV16 infection was relatively higher in the colorectal carcinoma and rare in the adjacent normal mucosa. Specimens expressing higher levels of HPV 16 E7 DNA were associated with lower Dukes stage and more distal location.


Subject(s)
Adenocarcinoma , Metabolism , Virology , Adult , Aged , Aged, 80 and over , Colorectal Neoplasms , Metabolism , Virology , DNA, Viral , Female , Genotype , Humans , Male , Middle Aged , Oncogene Proteins, Viral , Genetics , Papillomaviridae , Papillomavirus E7 Proteins , Papillomavirus Infections , Metabolism , Virology
14.
Article in Chinese | WPRIM | ID: wpr-542412

ABSTRACT

Objective To study the current research status of minichromosome maintenance protein 2 (MCM2), the cell cycle proliferation marker, in the diagnosis of colorectal carcinoma. Methods Literatures about the application of MCM2 in the study of colorectal carcinoma were collected and reviewed.Results MCM2, as a marker of cell dysplasia and malignancy, was usually used in the study of carcinoma. The study on expression of MCM2 in the cell of colorectum in different proliferational stage might help to screen colorectal carcinoma as early as possible. Conclusion As a relatively specific and sensitive marker of cell proliferation, MCM2 might become a promising mark for diagnosing colorectal carcinoma in the early stage.

15.
Article in Chinese | WPRIM | ID: wpr-675385

ABSTRACT

Objective To investigate the relationship between rules of local metastasis and the operation for rectal cancer. Methods Literatures about rules of local metastasis and operation for rectal cancer were reviewed and analysed. Results The study of rules of local metastasis results in the change and refinement of the operation for rectal cancer. Conclusion With the development of basic research for rectal cancer,the operation for rectal cancer is changing and refining, and the result of the treatment is more favourable.

16.
Article in Chinese | WPRIM | ID: wpr-582563

ABSTRACT

Objective To explore the characteristics of mycobacterium dependend antituberculous drug. Methods 137 strains of mycobacterium were tested for resistance by the absolute concentration method on Lowenstein Jensen medium. Dependent positive were the coloun that on higher concentration drug medium stronger than lower concentration than controls. Results 12(8.76%) of the 130 strains M. tuberculosis and 7 strains nontuberculous mycuberculous (NTM) were found dependent, in which strains dependend on INH, RFP and SM were 3(2.19%), 10(7.3%) and 3(2.19%) respectively, 4(2.92%) were dependend on two drugs. Conclusions Were NTM dependent, Not found all detected from multiple strains.

17.
Article in Chinese | WPRIM | ID: wpr-548504

ABSTRACT

Objective To investigate the organochlorine and pyrethroid pesticide residue in leafy vegetables on sale in Xi’an and to assess the safety of the leafy vegetables. Methods Eight kinds of leafy vegetables samples were collected at five wholesale markets and five supermarkets in Xi’an from 2007 to 2008. The content of 15 kinds of pesticides residues were analyzed by GC method and the evaluation was made according to GB 2763—2005. Results The rate of detection for the pesticides was 9.8% (465/4 725)and the disqualified rate was 1.2%(57/4 725).There was a significant seasonal difference in the detection rate of pesticides (P

18.
Article in Chinese | WPRIM | ID: wpr-544485

ABSTRACT

Objective To explore the feasibility for determination of chloroform and tetrachloromethane in drinking water with DB-5, Rtx-1 and DB-1 capillary columns. Methods For the mild solubility of chloroform and tetrachloromethane in drinking water, chloroform and tetrachloromethane were analysed by static headspace gas chromatography with DB-5, Rtx-1 and DB-1 capillary columns and electron capture detectorECD, external standard method was used for quantification. Results Three kinds of columns were used for determination of chloroform and tetrachloromethane,the retention time was lower than 3 min, for chloroform and tetrachloromethane the average recovery rates were 92.06%-104.95% and 78.33%-103.22% respectively, RSD were 2.06%-2.71% and 1.76%-5.59% respectively. Conclusion DB-5, Rtx-1 and DB-1 capillary columns are suitable for determination of chloroform and tetrachloromethane in drinking water.

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