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1.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 65-73, 2024.
Article in Chinese | WPRIM | ID: wpr-1013341

ABSTRACT

ObjectiveTo discuss the effects of Cistanches Herba phenylethanoid glycosides (CHPhGs) on the intestinal mucosal barrier and gut microbiota in alcoholic liver disease (ALD) mice were discussed. MethodThe 36 C57BL/6N female mice were randomly divided normal group, normal group of CHPhGs, model group, and low, medium, and high-dose groups (175, 350, 700 mg·kg-1) of CHPhGs, with six mice in each group. The ALD mouse model was built using Lieber-Decarli alcohol liquid feed. The normal group and low, medium, and high-dose groups of CHPhGs were given CHPhGs by gavage daily. Serum aspartate aminotransferase aminotransferase (ALT), alanine aminotransferase (AST), triglycerides (TG), and total cholesterol (TC) levels were detected by an automatic biochemical analyzer. Serum tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), lipopolysaccharide (LPS), lipopolysaccharide-binding protein (LBP), D-lactic acid (D-LA), diamine oxidase (DAO), and LBP of liver were detected by enzyme-linked immunosorbent assay (ELISA). The levels of TG and TC in the liver were detected by colorimetry. Liver tissue was treated by oil red O and hematoxylin-eosin (HE) staining. The microstructure of jejunum epithelial cells was observed by electron microscope. Jejunum and colon were treated by HE staining and alcian blue-periodate-scheff (AB-PAS) staining staining, and mucin 2 (Muc2) was treated by immunohistochemistry. The intestinal contents of the normal group, normal group of CHPhGs, model group, and high-dose group of CHPhGs were collected and sequenced. ResultThe ALD model was established successfully. Compared with the normal group, the levels of serum ALT, AST, and TG, as well as the levels of liver TG and TC in the model group were significantly increased (P<0.05). Histopathology showed that compared with the normal group, the liver cells in the model group showed obvious steatosis. Compared with the model group, the levels of serum TG and liver TG and TC in the low, medium, and high-dose groups of CHPhGs decreased significantly (P<0.05). The serum ALT, AST, TNF-α, IL-1β, LPS, and LBP in the high-dose group of CHPhGs were also significantly decreased (P<0.05). The number of liver cells with steatosis in the high-dose group of CHPhGs was significantly reduced, and the microvilli structure of jejunum epithelial cells was basically intact. The expression of Muc2 was reduced in the colon, and the gut microbiota of the high-dose group of CHPhGs changed significantly (P<0.05). Compared with the normal group, the Allobaculum was significantly up-regulated in the model group (P<0.05). Compared with the model group, the abundance of Akkermansia in the high-dose group of CHPhGs was significantly increased (P<0.01). The abundance of Akkermansia was negatively correlated with that of Allobaculum (r=-0.701, P<0.01). ConclusionCHPhGs can reduce the intestinal barrier injury caused by ALD, which may play a protective role by regulating the abundance and structure of Akkermansia and Allobaculum and affecting the homeostasis of intestinal mucus.

2.
Chinese Journal of Endocrinology and Metabolism ; (12): 315-319, 2023.
Article in Chinese | WPRIM | ID: wpr-994328

ABSTRACT

Objective:To explore the effect of triglyceride glucose(TyG) index, single nucleotide polymorphism of Toll-like receptor 4(TLR4) and NOD-like receptor thermal protein domain associated protein 3(NLRP3) genes, and its interaction on the risk of gout.Methods:A total of 315 male patients with gout and 499 men for health checkup at the same period were selected. General data were collected through questionnaires, and peripheral venous blood was collected for biochemical test. Three single nucleotide polymorphisms(SNPs) of NLRP3 and TLR4 were detected with multiplex ligase assay reaction, and logistic regression analysis was applied to compare the correlation between NLRP3 and TLR4 alleles and gout risk. The interaction of SNP and TyG index with gout was analyzed by generalized multi-factor dimensionality reduction(GMDR) model and logistic regression.Results:After adjusting for smoking, drinking, and other factors, the risk of gout increased by 61.1% for each standard deviation increase in TyG index. CC genotypes of rs10754558, rs10759932, and rs7525979 were high risk genotypes of gout in Han ethnicity. GMDR results showed significant differences in the interaction models of rs10754558-TyG index, rs7525979-TyG index, and rs10759932-TyG index between control group and gout group( P<0.05), suggesting an interaction between the three genotypes of SNPs selected and TyG index. Stratified analysis of the three selected SNPs and TyG index showed that after adjusting for age, smoking, and other factors, the high TyG index patients carrying C/C or C/G genotype at rs10754558 displayed an increased risk of gout compared with those carrying GG genotype and low TyG index( OR=2.127, P<0.05). Conclusion:The CC genotypes of rs10754558, rs10759932, and rs7525979 are high risk genotypes for gout in Han ethnicity. The interaction between rs10754558 and TyG index may increase the risk of gout development.

3.
Chinese Journal of Tissue Engineering Research ; (53): 2003-2008, 2017.
Article in Chinese | WPRIM | ID: wpr-614465

ABSTRACT

BACKGROUND:Breast cancer stem cells not only lead to theoccurrence of breast cancer, but also may cause breast cancer metastasis and recurrence. The relationship between stem cells and cell resistance is also gaining increasing attentions, and the focus on the stem cell treatment may result in unexpected results.OBJECTIVE:To explore the reversal effect of psoralen on glutathione-S-transferase π (GST-π) in human breast cancer MCF-7/ADR cells and its mechanism.METHODS:MCF-7/ADR cells were cultured and enriched in serum-free medium to obtain breast cancer stem cells.RT-PCR and western blot were used to detect the expression of GST-π at the levels of gene and protein in the MCF-7/ADR cells after treatment with 0, 4, 8, 12, 16 mg/L psoralen. To observe the activation of nuclear factor-κB,western blot was used. The expression of GST-π was detected by RT-PCR in 18 μmol/L SN50 group and 8 mg/L psoralen group. Cell counting kit-8 assay was used to detect the effect of doxorubicin on cell proliferation.RESULTS AND CONCLUSION:Compared with the control group, psoralen reduced the expression of GST-π at the mRNA and protein levels, and significantly inhibited the activation of nuclear factor-κB. It was suggested that psoralen could reverse the multidrug resistance of human breast cancer MCF-7/ADR stem cells by decreasing the expression level of GST-π. The mechanism may be achieved by inhibiting the nuclear factor-κB signal pathway.

4.
The Journal of Practical Medicine ; (24): 1857-1860, 2017.
Article in Chinese | WPRIM | ID: wpr-612094

ABSTRACT

Objective To investigate the differences between indocyanine green (ICG) fluorescence imaging plus methylene blue and plus Carbon Nanoparticles Suspension Injection for sentinel lymph node biopsy (SLNB)in breast cancer patients. Methods A total of 134 cases of early breast cancer patients performed SLNB from November 2013 to November 2016 were involved,of which 48 cases were performed with ICG fluorescence imaging plus methylene blue,and another 86 cases plus Carbon Nanoparticles Suspension Injection. Results There was no significant difference between ICG plus Methylene Blue group and ICG plus nano carbon group in terms of detection rate(P>0.05),detected numbers(P>0.05),sensitivity(P>0.05),accuracy(P>0.05)and false negative rate(P > 0.05). Age,and body mass index(BMI)exerted no influence on the detection rate and accuracy of SLNB in two groups(P>0.05). Conclusion ICG Fluorescence Imaging plus Methylene Blue showed similar detection rate , detected numbers , sensitivity , accuracy and false negative rate as it plus Carbon Nanoparticles Suspension Injection for SLNB in breast cancer patients ,and both of them can be performed easily and conveniently.

5.
Journal of International Oncology ; (12): 444-446, 2011.
Article in Chinese | WPRIM | ID: wpr-415188

ABSTRACT

Breast cancer stem cells (BCSC)are a class of breast cancer cells that have the capacity of selfrenewal and can differentiate into different cell lineages. These cells, with the ability of high tumorigenesis, invasion and metastasis, play a important role in breast cancer metastasis, recurrence and treatment resistance. The treatments targeting on breast cancer stem cells are very important for improving the efficacy of clinical therapy.

6.
Chinese Pharmacological Bulletin ; (12)2003.
Article in Chinese | WPRIM | ID: wpr-564230

ABSTRACT

Aim To study the anti-inflammatory effects and mechanisms of SBM isolated from Scutellariae radix by high hydrostatic pressure.Methods The effect of SBM on ConA-induced splenocyte proliferation and LPS-induced IL-1? production from peritoneal macrophage was studied in vitro,and the therapeutic effects of SBM on adjuvant-induced arthritis,formalin-induced paw oedema and acetic acid-induced vascular permeability were also investigated.Results SBM(31.25~500 mg?L-1)significantly inhibited ConA-induced splenocyte proliferation,IL-1? production from peritoneal macrophages in vitro.SBM 20 mg?kg-1 inhibited primary and secondary inflammatory reaction,decreased the elevated level of IL-1? released from peritoneal macrophages,inhibited splenocyte proliferation in adjuvant-induced arthritis in rats.Moreover,SBM inhibited formalin-induced paw oedema and acetic acid-induced vascular permeability.Conclusions SBM could inhibit inflammatory reaction.Its mechanisms might be related to the suppression of immune function and inhibition on proinflammatory cytokines production.

7.
Chinese Journal of Cancer Biotherapy ; (6)1995.
Article in Chinese | WPRIM | ID: wpr-583878

ABSTRACT

Objective: To investigate the inhibitory effects and mechanism of a novel anti-human DR5 ( death receptor 5 of TRAIL) monoclonal antibody to glioma cell lines U343. Methods: DR5 protein was tested quantitative through FCM: DR5 mRNA was observed through RT-PCR and distribution was tested by immunocytochemistry. Inhibitory effects and inducing apoptosis of anti-human DR5 monoclonal antibody to U343 were analysed by MTT, DNA Ladder, FCM. Results: The expression of death receptor 5 ( DR5 ) was certificated in U343 , DR5 appeared to be located in intracellular perinucle-ar compartment. Inhibitory effects of anti-human DR5 monoclonal antibody on U343 were achieved by 3 ?g/ml at 4 hours and the mechanism was associated with apoptosis. Conclusion: Apoptosis of glioma cell lines U343 can be induced by anti-human DR5 monoclonal antibody, and targeted on DR will provide new way to treating cancer.

8.
Chinese Journal of Ultrasonography ; (12)1993.
Article in Chinese | WPRIM | ID: wpr-540946

ABSTRACT

the upper-nether distance,4 cases with round-like defects((23.5%)),2 cases with irregular defects((11.8%)).The maximal diameters obtained from 2-DE were significantly lower than those from RT-3DE(r=(0.72),Pthe upper-nether distance.But the sizes measured by 2-DE are all the upper-nether distance,so the real size of ASD often underestimated.

9.
Chinese Pharmacological Bulletin ; (12)1987.
Article in Chinese | WPRIM | ID: wpr-565085

ABSTRACT

Aim To study the anti-inflammatory effects and mechanisms of SBM isolated from Scutellariae radix by high hydrostatic pressure.Methods The effect of SBM on ConA-induced splenocyte proliferation,LPS-induced PGE2 production and protein expression of COX-2 were studied in vitro,and the therapeutic effects of SBM on carragineen-induced paw oedema and acetic acid-induced twisting reaction were also investigated.Results SBM(15.63~250) mg?L-1 significantly inhibited LPS-induced PGE2 production and COX-2 enzyme activity in peritoneal macrophages in mice.SBM(125 mg?L-1)also inhibited ConA-induced protein phosphorylation of p38 MAP kinase and LPS-induced COX-2 protein expression.SBM(10,20 mg?kg-1)inhibited carragineen-induced paw oedema and acetic acid-induced twisting reaction.Conclusion SBM can inhibit inflammatory reaction.Its mechanisms may be related to the suppression of inflammatory medium production.

10.
Chinese Journal of Pathophysiology ; (12)1986.
Article in Chinese | WPRIM | ID: wpr-530307

ABSTRACT

AIM:To explore the effects of pentoxifylline (PTX) on ventricular remodeling and cardiac function in dilated cardiomyopathy (DCM) rats.METHODS: Lewis rats were randomly allocated to a myocin-induced dilated cardiomyopathy (DCM) group receiving saline (n=10), a DCM group receiving PTX (PTX group; 25 mg?kg-1?d-1, ip, for 30 days, n=10) or healthy control group (n=10). The levels of tumor necrosis factor-? (TNF-?), interleukin-6 (IL-6) and IL-10 in the blood plasma were analyzed by ELISA. The extent of fibrosis was estimated using Masson's staining and immunohistochemistry analyses. Cardiac structure and function were measured by echocardiography.RESULTS: PTX decreased plasma levels of TNF-? and IL-6, and increased IL-10 level in DCM animals compared with DCM group [TNF-?: (7.21?0.24) ?g/L vs (19.30?1.31) ?g/L, P

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