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1.
Article in English | WPRIM | ID: wpr-827430

ABSTRACT

OBJECTIVES@#To establish an electrophysiological model of alcoholic cardiomyopathy by inducing pluripotent stem cells (iPSCs) to differentiate into cardiomyocytes (iPSC-CM) in vitro.@*METHODS@#The human iPSC were expanded in vitro and differentiated into iPSC-CM. The iPSC-CM were divided into a blank control group, an alcoholic experiment group (according to the concentration of alcoholic, the alcoholic experiment was also divided into many subgroups), and a KN93 treatment group. Then the efficiency of iPSC differentiated to iPSC-CM was detected by immunofluorescence, the function of iPSC-CM was detected by cell counting kit-8 (CCK8) assay and lactate dehydrogenase (LDH) activity assay kit. The electrophysiological activity of iPSC-CM was monitored by real time cellular analysis (RTCA), the injury of iPSC-CM caused by alcohol was further verified by the mitochondrial membrane potential fluorescence probe JC-1 staining combined with RTCA analysis.@*RESULTS@#Compared with the blank control group, the different doses (25, 50, 100, 150, 200, 250, 300 mmol/L) of alcohol could significantly inhibit the proliferation of iPSC-CM in a dose-dependent manner (all <0.05). Compared with the blank control group, the activity of iPSC-CM was significantly reduced by 100 mmol/L alcohol, resulting in the increase of LDH release, the decrease of mitochondrial membrane potential, the amplitude and beating rate (all <0.05). Compared with the 100 mg/mL alcoholic experiment group, the KN93 treatment group significantly alleviated the damage of alcohol to iPSC-CM by blocking the necrotic apoptotic pathway, resulting in the decrease of LDH release, the increase of mitochondrial membrane potential, the amplitude and beating rate (all <0.05).@*CONCLUSIONS@#The electrophysiological model of alcoholic cardiomyopathy based on the differentiation of cardiomyocytes are successfully established, which can be used to study the electrophysiological activity and the molecular mechanism for relevant diseases, and it may provide a more reasonable and effective research tool for drug screening and clinical study.


Subject(s)
Cardiomyopathy, Alcoholic , Cell Differentiation , Electrophysiological Phenomena , Humans , Induced Pluripotent Stem Cells , Myocytes, Cardiac
2.
Article in Chinese | WPRIM | ID: wpr-870174

ABSTRACT

Coronavirus disease 2019 (COVID-19) can cause great damage to the elderly patients and lead to high mortality. The clinical presentations and auxiliary examinations of the elderly patients with COVID-19 are atypical, due to the physiological ageing deterioration and basal pathological state. The treatment strategy for the elderly patients has its own characteristics and treatment protocol should be considered accordingly. To improve the diagnosis, treatment, and prevention of COVID-19 in the elderly, the Expert Committee of Geriatric Respiratory and Critical Care Medicine, China Society of Geriatrics established the "Expert consensus for the diagnosis, treatment, and prevention of coronavirus disease 2019 in the elderly" . We focused on the clinical characteristics and key points for better treatment and prevention of COVID-19 in the elderly. (1) For diagnosis, atypical clinical presentation of COVID-19 in the elderly should be emphasized, which may be complicated by underlying disease. (2) For treatment, strategy of multiple disciplinary team (mainly the respiratory and critical care medicine) should be adopted and multiple systemic functions should be considered. (3) For prevention, health care model about integrated management of acute and chronic diseases, in and out of hospital should be applied.

3.
Article in Chinese | WPRIM | ID: wpr-867027

ABSTRACT

Objective:To study whether aspirin has inhibitory effect on microglia activation induced by Poly-IC and its mechanism.Methods:Microglia cell line BV2 were cultured in vitro to establish a Poly-IC stimulation-induced microglia cell immune activation model. The experiment groups were divided into control group (no treatment), model group (Poly-IC 10 μg/ml), high dose aspirin group (1 mmol/L aspirin), low dose aspirin group (0.1 mmol/L aspirin), high dose aspirin pretreatment group (Poly-IC 10 μg/ml + 1 mmol/L aspirin) and low dose aspirin pretreatment group (Poly-IC 10 μg/ml + 0.1 mmol/L aspirin). The phagocytosis ability of microglia cells, reactive oxygen species (ROS) and Iba1 protein expression were detected by using immunofluorescence method. The expression of the inflammatory cytokines Il-1β, Il-6, Il-10, TNF-α and cox-2 mRNA in microglia cells were detected by real-time quantitative PCR (RT-qPCR).Results:Compared with the control group, the morphology of microglia cells in model group changed significantly, and the phagocytosis ability and production of reactive oxygen species (ROS) increased. At the meantime, the expression of Iba1 protein was strongly decreased. In the model group, The mRNA expressions of IL-1β(20.55±1.92), IL-6 (63.98±7.83), TNF-α (16.84±3.19), COX-2 (6.78±0.42) were higher than IL-1β(1.01±0.14), IL-6 (0.95±0.17), TNF-α (1.22±0.38), COX-2 (0.87±0.11) in the control group. (Il-1β ( t=26.14), Il-6 ( t=10.22), TNF-α ( t=17.06) and COX-2 ( t=37.07), all P<0.01). In the aspirin pretreatment group, the phagocytic ability of microglia cells was inhibited compared with the model group, and the production of reactive oxygen species (ROS) reduced. The expression of Iba1 protein was also partly recovered. Meanwhile, the effect of the high aspirin dose pretreatment group on pro-inflammatory factors IL-1β(9.95±0.52), IL-6 (39.64±6.89), TNF-α(1.57±0.42), COX-2 (2.47±0.14)were lower than those in the model group significantly.(IL-1β: t=14.18, IL-6: t=3.69, TNF-α: t=16.68, COX-2: t=27.03, all P<0.01). Conclusion:Aspirin has an inhibitory effect on microglial activation induced by Poly-IC, which may be related with inhibiting the expression of inflammatory factors.

4.
Chinese Critical Care Medicine ; (12): 686-690, 2020.
Article in Chinese | WPRIM | ID: wpr-866894

ABSTRACT

Objective:To investigate the level and changing trend of microparticles (MPs) in super-elderly infected patients, and explore its early warning effect on infection.Methods:The infected patients ≥ 85 years old admitted to the Second Medical Center of Chinese PLA General Hospital from December 2018 to March 2019 were selected as the observation group, and the healthy volunteers ≥ 85 years old in the same period were selected as the control group. Venous blood samples were collected at the 2nd hour, the 2nd day and the 7th day after fever, and the inflammatory markers such as white blood cell count (WBC), neutrophil percentage (NEUT), C-reactive protein (CRP) and procalcitonin (PCT) were measured. The levels of MPs were determined by flow cytometry. AnnexinⅤlabeled CD11b positive MPs (AnnexinⅤ +/CD11b + MPs) represented leukocyte microparticles (LMPs), and AnnexinⅤlabeled CD66b positive MPs (AnnexinⅤ +/CD66b + MPs) represented neutrophil microparticle (NMPs). The differences of each index at different time points between the two groups were compared, and the predictive value of each index to the infection of elderly patients was analyzed by receiver operating characteristic (ROC) curve. Results:A total of 38 subjects were enrolled, including 28 cases in the observation group and 10 cases in the control group. The levels of LMPs and NMPs in the observation group increased to the peak at the 2nd hour after fever, and were significantly higher than those in the control group [LMPs (cells/μL): 55.0 (28.8, 197.2) vs. 19.0 (13.5, 28.3), NMPs (cells/μL): 226.5 (123.3, 516.5) vs. 26.5 (22.0, 48.8), both P < 0.01]. With the control of the disease, LMPs and NMPs decreased gradually. The NMPs on the 2nd day was significantly lower than that at the 2nd hour of fever [cells/μL: 106.0 (40.0, 309.0) vs. 226.5 (123.3, 516.5), P < 0.05], and the LMPs and NMPs on the 7th day were significantly lower than those on the 2nd day [LMPs (cells/μL): 17.0 (12.5, 43.8) vs. 42.0 (13.0, 117.0), NMPs (cells/μL): 30.0 (15.8, 62.0) vs. 106.0 (40.0, 309.0), both P < 0.05]. There was no significant difference in the levels of LMPs and NMPs between the two groups on the 7th day. Among the inflammatory markers, the NEUT in the observation group was significantly higher than that in the control group at the 2nd hour of fever (0.70±0.09 vs. 0.59±0.04, P < 0.01), but there was no significant difference in WBC, CRP and PCT between the two groups. On the 2nd day, the inflammatory markers in the observation group reached the peak and were significantly higher than those in the control group [WBC (×10 9/L): 9.33±2.44 vs. 6.37±1.28, NEUT: 0.78±0.08 vs. 0.57±0.04, CRP (mg/L): 5.67±2.99 vs. 0.33±0.18, PCT (μg/L): 0.80±0.67 vs. 0.07±0.03, all P < 0.01]. On the 7th day, the inflammatory markers in the observation group decreased significantly, and there was no significant difference between the observation group and the control group. ROC curve analysis showed that the area under ROC curve (AUC) and 95% confidence interval (95% CI) of LMPs and NMPs on the day of fever were higher than those of WBC, NEUT, CRP and PCT [0.888 (0.763-1.000), 0.973 (0.931-1.000) vs. 0.679 (0.346-0.811), 0.829 (0.700-0.958), 0.607 (0.404-0.811), 0.554 (0.358-0.749)]. Conclusion:LMPs and NMPs are significantly increased in the early stage of fever, which can predict the incidence of infection in the super-elderly patients.

5.
Article in Chinese | WPRIM | ID: wpr-864740

ABSTRACT

Objective:To explore the effect of International Classification of Functioning, Disability and Health, Children and Youth Version (ICF-CY) framed oral motor therapy combined with acupoint massage intervention on children with cerebral palsy swallowing disorder.Methods:From September 2018 to September 2019, 80 children with cerebral palsy accompanied by dysphagia who were diagnosed and treated in the Department of Neurological Rehabilitation of our hospital were selected as the study subjects, and were divided into the experimental group (40 cases) and the control group (40 cases) by random number table method. A total of 76 patients completed the study. On the basis of routine swallowing training and oral movement intervention, the experimental group was comprehensively evaluated from the aspects of body function and structure, activity and participation, and environmental factors with ICF-CY theory as the framework, and was given oral and facial acupoint massage for 12 weeks. The control group was given conventional swallowing training and oral exercise intervention for 12 weeks. The modified water swallow test was used to evaluate the effect of improving swallowing disorder in two groups of children after 12 weeks of intervention. The improvement of oral motor function in two groups of children after 4 and 12 weeks of intervention was evaluated by using the Grading Criteria for Evaluation of Oral Motor Function.Results:After 12 weeks of intervention, the total effective rate of swallowing disorder improvement in the experimental group and the control group were 89.47% (34/38) and 63.16% (24/38), respectively, and the difference between the two groups was statistically significant ( χ2 value was 7.28, P<0.05). After 4 and 12 weeks of intervention, the functional scores of oral motors in the experimental group were (67.13±2.35) and (82.08±2.33) points, respectively, which were higher than those of the control group at the same time (56.16±2.57) and (65.63±2.54) points, and the differences were statistically significant ( t values were -3.320, -4.433 P<0.05). After 4 and 12 weeks of intervention, the children's functional scores of oral motors in each group were higher than those in each group before intervention. Compared with the group before intervention, t values of the experimental group were -5.377 and -5.376; t values of the control group were -10.715 and -17.486; compared to the group with 4-week intervention, t value of the experimental group was -12.732; t value of the control group was -13.281, all the differences were statistically significant (P<0.05). The results of repeated measures analysis of variance showed that the time effect, grouping effect, and the interaction between time effect and grouping effect of functional scores of oral motors were statistically significant ( F values were 426.780, 9.084, 26.538, P < 0.05). Conclusions:Oral motor therapy combined with acupoint massage based on ICF-CY framework can effectively promotes the recovery and reconstruction of mouth perception and motor function in children with cerebral palsy, significantly improves the children's dysphagia and their ability to actively participate in feeding.

6.
Chinese Journal of Hepatology ; (12): 358-362, 2019.
Article in Chinese | WPRIM | ID: wpr-810627

ABSTRACT

Objective@#To explore the correlation between serum 25-hydroxyvitamin D3 (25[OH]D3) levels and esophageal variceal bleeding (EVB) in cirrhotic patients.@*Methods@#Eighty-three cases with liver cirrhosis hospitalized from November 2016 to January 2017 were collected. The patients were divided into bleeding group (51 cases) and non-bleeding group (32 cases) depending on the presence or absence of bleeding under gastroscopy. Serological tests were performed on both groups, including hemoglobin (Hb), albumin (ALB), alkaline phosphatase (ALP),γ-glutamyltransferase (GGT), interleukin-6 (IL-6), and 25-hydroxyvitamin D3 (25[OH]D3). Both groups were analyzed by univariate analysis. The differences between both groups were compared by t-test, after normality test. The other variables were compared by Mann-Whitney U test. The correlation between the relevant variables and EVB were analyzed by Spearman's rank correlation and a multivariate analysis. Cases with primary biliary cirrhosis were relatively low in number (four cases in bleeding group, accounting for 8%, 10 cases in non-bleeding group, accounting for 31%). The effects of ALP and GGT on serum 25(OH)D3 level were analyzed by stratified analysis. Moreover, ALP and GGT levels were divided into two and three groups: < 140 U/L and >140 U/L and < 30 U/L, > 30 U/L, and ~≤60 U/L.@*Results@#Bleeding group had low levels of hemoglobin (t= -2.827,P= 0.005), alkaline phosphatase (t= -3.097,P= 0.002), gamma-glutamyltransferase (t= -2.292,P= 0.022), and 25(OH)D3 (t= -3.134,P= 0.002) than non-bleeding group. Both groups (P> 0.05) had similar levels of albumin, interleukin-6, AAR, and FIB-4. Logistic regression analysis showed that 25(OH)D3, alkaline phosphatase and hemoglobin were independent risk factors for EVB. Spearman’s correlation coefficient analysis showed that 25(OH)D3was significantly positively and negatively correlated with interleukin-6 (r= 0.306,P= 0.005) and albumin (r= -0.327,P= 0.003). Stratified analysis showed that serum 25(OH)D3 level was lower in ALP≤140U/L group and the bleeding group, and the difference was statistically significant than non-bleeding group (P= 0.007), while the serum level of 25(OH)D3was decreased in both groups for alkaline phosphatase > 140 U/L group, and the difference was not statistically significant (P= 0.051). Furthermore, in the GGT > 60 U/L group, the serum level of 25(OH)D3was significantly lower in the bleeding group, and the difference was statistically significant in non-bleeding group (P= 0.003), while the difference between the two groups was not statistically significant (P> 0.05) in GGT≤30 U/ L, > 30 U/L, and ~≤60 U/L group.@*Conclusion@#Serum 25(OH)D3level was significantly lower in EVB cirrhotic patients, and it was an independent risk factor for EVB. Serum 25(OH)D3 low levels was more apparent with ALP normalization or GGT level > 60 U/L.

7.
Article in Chinese | WPRIM | ID: wpr-756479

ABSTRACT

Objective To propose and validate a reduced volume β-quantification method to measure serum high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C). Methods The reduced volume β-quantification method involved separation of LDL and HDL by ultracentrifugation and preparation of HDL by chemical precipitation. The sampling and reconstitution of the bottom fractions were performed gravimetrically and sample volume was thus decreased from 5 to 0.8 ml. High performance liquid chromatography was used to determine the cholesterol concentration of bottom fractions and HDL-C in the supernatant. Serum levels of LDL-C depended on a calculation of bottom fractions cholesterol minus HDL-C. Results The total CVs for HDL-C and LDL-C were 0.65% -1.75% and 0.63% -1.11%. The results of the developed method were consistent with the current reference method and well within the allowable bias for Cholesterol Reference Method Laboratory Network surveys. Conclusion A new method for the measurement of HDL-C and LDL-C has been established. This method requires a small amount of serum and is easy to operate, exhibiting a desirable precision and accuracy. It is reliable and can be used as a candidate reference method for HDL-C and LDL-C.

8.
Article in Chinese | WPRIM | ID: wpr-755950

ABSTRACT

Objective To explore the effects of dexmedetomidine on myocardial injury during liver cold ischemia reperfusion in rats .Methods A total of 40 healthy male Sprague-Dawley (SD)rats with a weight of 220~250 gram and an age of 8~10 weeks were randomly divided into 5 groups of sham ,model ,Dex ,Atip and AG490 by a random number table (n= 8 each) .At 8h post-reperfusion , blood samples were harvested from infra-hepatic vena cava and serum levels of tumor necrosis factor-alpha (TNF-α) ,interleukin-6 (IL-6) ,creatine kinase-muscle/brain (CK-MB) ,troponin I (cTnI)and heart-type fatty acid binding protein (H-FABP )determined by enzyme-linked immunosorbent assay (ELISA ) .After blood sampling ,the rats were sacrificed ,the expression of activated caspase-3 was detected by immunohistochemistry and apoptotic cells by TUNEL .Apoptotic rate was calculated .And the phosphorylations of JAK2 ,STAT1 and STAT3 were assessed by Western blot .Results As compared with sham group ,the levels of TNF-α,IL-6 ,CK-MB ,cTnI and H-FABP significantly increased ,apoptotic rate spiked ,pathological damage worsened and the expressions of activated caspase-3 ,p-JAK2 ,p-STAT1 and p-STAT3 were up-regulated in other groups ( P< 0 .05 );As compared with model group ,the levels of TNF-α,IL-6 ,CK-MB ,cTnI and H-FABP significantly decreased ,apoptotic rate declined ,pathological damage became alleviated and the expressions of activated caspase-3 ,p-JAK2 ,p-STAT1 and p-STAT3 became down-regulated in groups Dex and AG490 (P<0 .05);as compared with group Dex ,the levels of TNF-α,IL-6 ,CK-MB ,cTnI and H-FABP significantly increased ,apoptotic rate rose ,pathological damage worsened and the expressions of activated caspase-3 ,p-JAK2 ,p-STAT1 and p-STAT3 became up-regulated in group Atip (P<0 .05) . Conclusions Dexmedetomidine can ameliorate myocardial injury induced by liver cold ischemia-reperfusion in rats .

9.
Article in Chinese | WPRIM | ID: wpr-755917

ABSTRACT

Objective To evaluate the effect of propofol and sevoflurane on acute kidney function in infants undergoing pediatric parent liver transplantation.Methods 80 pediatric patients scheduled for pediatric liver transplantation were randomly assigned to receive either continuous infusion of propofol or inhalation of sevoflurane.Serum creatinine (Scr),inflammatory medium and renal biomarkers were measured before surgery (T1),30min after anhepatic phase (T2),3h after ischemia reperfusion (T3),36 h after surgery (T4) to evaluate the effect of anesthetics on the development of postoperative kidney injury.Results Overall,compared Propofol group,the mean arterial pressure changed slightly in sevoflurane group.The inflammatory factors of Scr,IL-18,TNF-α,and levels of NGAL were lower in sevoflurane group while no differences of IL-10 were found between both groups.Conclusions Compared with propofol anesthesia,the degree of renal injury in pediatric living donor liver transplantion under sevoflurane anesthesia is reduced,which may be related to hemodynamic stability and decreased release of inflammatory factors.

10.
Article in Chinese | WPRIM | ID: wpr-755554

ABSTRACT

Objective To compare the effects of sevoflurane-and propofol-based anesthesia on kid-ney injury in pediatric patients undergoing living donor liver transplantation. Methods Eighty pediatric pa-tients of both sexes, aged 5-15 months, weighing 5. 5-10. 0 kg, of American Society of Anesthesiologists physical status Ⅱ or Ⅲ, scheduled for elective living donor liver transplantation, were divided into 2 groups ( n=40 each) using a random number table method: sevoflurane-based anesthesia group ( group S) and propofol-based anesthesia ( group P ) . Sevoflurane was inhaled, and the end-tidal concentration was maintained at 1. 0%-3. 0% in group S. Propofol 9-15 mg·kg-1 ·h-1 was intravenously infused in group P. Fentanyl 1-3 μg∕kg was intermittently injected, and cisatracurium 1-2 μg·kg-1·min-1 was intrave-nously infused in two groups. Bispectral index value was maintained at 40-60. Immediately before skin in-cision ( T1 ) , at 30 min of anhepatic phase ( T2 ) , at 3 h of neohepatic phase ( T3 ) , at 24 h after operation ( T4 ) and at day 3 after surgery ( T5 ) , blood samples from the central vein and urine specimens were col-lected for determination of the levels of neutrophil gelatinase-associated lipocalin and cystatin C in serum and urine by enzyme-linked immunosorbent assay. The urine volume, requirement for dopamine and occurrence of hypotension and myocardial ischemia were recorded during surgery. Results Compared with group P, the levels of neutrophil gelatinase-associated lipocalin and cystatin in serum and urine were significantly de-creased at T3-5 ( P<0. 05) , and no significant change was found in the intraoperative urine volume, inci-dence of hypotension and myocardial ischemia, or requirement for dopamine in group S ( P>0. 05) . Con-clusion Compared with propofol-based anesthesia, kidney injury is attenuated in pediatric patients under-going living donor liver transplantation under sevoflurane-based anesthesia.

11.
Article in Chinese | WPRIM | ID: wpr-751540

ABSTRACT

Objective The incidence and distribution of positional deformities ( PD) were studied by measuring cranial types of infants aged 0-6 months of 3 hospitals in Chongqing, and the recommended values of local PD were discussed. Methods Data of 3406 infants with 0-6 months head size measurements were col-lected from the Second Affiliated Hospital of Army Military Medical University, Yongchuan Maternal and Child Health Care Hospital and Wanzhou Maternal and Child Health Care Hospital from September 1, 2017 to August 31, 2018, cranial vault asymmetry ( CVA) and cranial index ( CI) were calculated. According to different age groups, observe the distribution of cranial types, and use the current international common diagnostic crite-ria to understand the incidence of cranial abnormalities, and analyze the distribution of percentile values of CVA and CI in infants in Chongqing. Results According to the current international standard diagnosis of PD, the incidence of simple brachycephaly was the highest ( 39. 9%) in the overall cranial abnormalities of infants, followed by brachycephaly with plagiocephaly was the second (36. 1%), simple plagiocephaly was the lowest (6. 9%), and normal cranial type only accounted for 17. 1%. The detection rates of plagiocephaly (36. 9%)and brachycephaly ( 67. 0%) were the lowest in the 0-2 months group, and the highest in the 3-4 months group (47. 3%/83. 3%), and then decreased in the 5-6 months group (46. 6%/80. 2%). Conclusion The basic values of cranial type measurement of full-term infants in chongqing were significantly different from inter-national reports, mainly due to the large CI value. The general international diagnosis standard for positional deformities do not conform to Chinese infants. We suggest that the reference value for positional deformities in this region is as follows: CVA≥0. 4 cm is abnormal, of which 0. 4 cm≤CVA<0. 6 cm for mild plagiocepha-ly, 0. 6 cm≤CVA<1. 0 cm for moderate plagiocephaly, CVA≥1. 0 cm for severe plagiocephaly; 82%≤CI≤91% is abnormal, of which 91%≤CI <94% for mild brachycephaly; 94% ≤CI <99% for moderate brachycephaly; CI≥99% for severe brachycephaly. CI≤82% is scaphocephaly.

12.
Article in Chinese | WPRIM | ID: wpr-798213

ABSTRACT

Objective@#The incidence and distribution of positional deformities(PD)were studied by measuring cranial types of infants aged 0-6 months of 3 hospitals in Chongqing, and the recommended values of local PD were discussed.@*Methods@#Data of 3 406 infants with 0-6 months head size measurements were collected from the Second Affiliated Hospital of Army Military Medical University, Yongchuan Maternal and Child Health Care Hospital and Wanzhou Maternal and Child Health Care Hospital from September 1, 2017 to August 31, 2018, cranial vault asymmetry(CVA)and cranial index(CI)were calculated.According to different age groups, observe the distribution of cranial types, and use the current international common diagnostic criteria to understand the incidence of cranial abnormalities, and analyze the distribution of percentile values of CVA and CI in infants in Chongqing.@*Results@#According to the current international standard diagnosis of PD, the incidence of simple brachycephaly was the highest(39.9%)in the overall cranial abnormalities of infants, followed by brachycephaly with plagiocephaly was the second(36.1%), simple plagiocephaly was the lowest(6.9%), and normal cranial type only accounted for 17.1%.The detection rates of plagiocephaly(36.9%)and brachycephaly(67.0%)were the lowest in the 0-2 months group, and the highest in the 3-4 months group(47.3%/83.3%), and then decreased in the 5-6 months group(46.6%/80.2%).@*Conclusion@#The basic values of cranial type measurement of full-term infants in chongqing were significantly different from international reports, mainly due to the large CI value.The general international diagnosis standard for positional deformities do not conform to Chinese infants.We suggest that the reference value for positional deformities in this region is as follows: CVA≥0.4 cm is abnormal, of which 0.4 cm≤CVA<0.6 cm for mild plagiocephaly, 0.6 cm≤CVA<1.0 cm for moderate plagiocephaly, CVA≥1.0 cm for severe plagiocephaly; 82%≤CI≤91% is abnormal, of which 91%≤CI<94% for mild brachycephaly; 94% ≤CI<99% for moderate brachycephaly; CI≥ 99% for severe brachycephaly.CI≤82% is scaphocephaly.

13.
Article in Chinese | WPRIM | ID: wpr-745657

ABSTRACT

Objective To investigate the effect of dexmedetomidine pretreatment on Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway during myocardial injury induced by liver ischemia-reperfusion (I/R) in rats.Methods Twenty-four healthy adult male SpragueDawley rats,aged 8-10 weeks,weighing 220-250 g,were divided into 3 groups (n=8 each) using a random number table method:sham operation group (group S),liver I/R group (group I/R),and dexmedetomidine pretreatment group (group D).The portal vein,superior and inferior vena cava,subhepatic inferior vena cava and hepatic artery were clamped,and the liver was perfused with 4 ℃ lactated Ringer's solution for 60 min through the portal vein to establish the model of liver cold I/R in anesthetized rats.Dexmedetomidine 100 μg/kg was intraperitoneally injected at 30 min before ischemia in group D.Blood samples were collected at 8 h of reperfusion from the inferior vena cava for determination of serum cardiac troponin Ⅰ (cTnⅠ) and heart-type fatty acid binding protein (H-FABP) concentrations (using the automatic biochemistry analyzer),tumor necrosis factor-alpha (TNF-α) and high-mobility group box 1 protein (HMGB1) concentrations (by enzyme-linked immunosorbent assay).The rats were then sacrificed,andhearts were harvested for examination of histopathological changes (with a light microscope) and for determination of the malondialdehyde (MDA) content (using thiobarbituric acid method) and superoxide dismutase (SOD) activity (by xanthine oxidase method),and expression of phosphorylated STAT1 and STAT3 (p-STAT1,p-STAT3) and phosphorylated JAK2 (p-JAK2) in myocardial tissues (by Western blot).Results Compared with group S,the serum concentrations of cTnI,H-FABP,TNF-α and HMGB1 were significantly increased,the MDA content was increased,the SOD activity was decreased,and the expression of p-JAK2,p-STAT1 and p-STAT3 was up-regulated in I/R and D groups (P<0.05).Compared with group I/R,the serum concentrations of cTnI,H-FABP,TNF-α and HMGB1 were significantly decreased,the MDA content was decreased,the SOD activity was increased,the expression of pJAK2,p-STAT1 and p-STAT3 was down-regulated (P<0.05),and pathological changes of myocardium were significantly attenuated in group D.Conclusion The mechanism by which dexmedetomidine pretreatment mitigates myocardial injury induced by liver cold I/R may be related to inhibiting activation of JAK/STAT signaling pathway in rats.

14.
International Journal of Surgery ; (12): 277-280, 2019.
Article in Chinese | WPRIM | ID: wpr-743036

ABSTRACT

The gastrointestinal flora is composed of a large number of microorganisms with complex structure,which participates in the process of decomposition,digestion and absorption of nutrients,promotes the development of the body's immune system,inhibits the colonization of pathogenic bacteria,and affects the health and disease of the body.In recent years,with the advancement of sequencing technology,the relationship between gastrointestinal flora and gastrointestinal neoplasms has became a research hotspot.This article reviews the carcinogenic pathways of the gastrointestinal flora and the possible carcinogenic mechanisms of the gastrointestinal flora.

15.
China Pharmacy ; (12): 1571-1576, 2019.
Article in Chinese | WPRIM | ID: wpr-816927

ABSTRACT

OBJECTIVE: To systematically evaluate therapeutic efficacy of Zinc preparation for radiation-induced oral mucositis (ROM) in head and neck cancers, and to provide evidence-based reference for clinical treatment. METHODS: Retrieved from PubMed, Medline, Embase, the Cochrane library, CJFD,VIP and Wanfang database, RCTs about additional use of Zinc preparation (trial group) in adjunctive treatment of ROM in head and neck cancers base on routine treatment or blank control (control group) were collected during database establishment to Dec. 2018. After data extraction and quality evaluation with Cochrane system evaluator manual 5.1.0, Meta-analysis was carried out for the incidence of ROM within 2 weeks after medication, total incidence of ROM and the incidence of serve ROM by using Rev Man 5.3 statistical software. RESULTS: A total of 8 RCTs were included, involving 550 patients. Results of Meta-analysis showed that there was no statistical significance in the incidence of ROM within 2 weeks [OR=0.55, 95%CI(0.26,1.17), P=0.12], total incidence of ROM [OR=0.59, 95%CI(0.08,4.39), P=0.60] or the incidence of serve ROM [OR=0.58, 95%CI(0.23,1.47), P=0.25] between 2 group. CONCLUSIONS: Based on routine therapy or blank control, additional use of Zinc preparation can not reduce the incidence and control the development of ROM in head and neck cancers.

16.
Article in Chinese | WPRIM | ID: wpr-774165

ABSTRACT

experimental test for mechanical properties of a vascular stent is a main method to evaluate its effectiveness and safety, which is of great significance to the clinical applications. In this study, a comparative study of planar, V-groove and radial compression methods for the radial support property test were performed, and the effects of compression rate and circumferential position on the test results were conducted. Based on the three-point bending method, the influences of compression rate and circumferential position on flexibility were also explored. And then a best test proposal was selected to evaluate the radial support property and flexibility of the three self-designed stents and the comparative biodegradable vascular stent (BVS) (BVS1.1, Abbott Vascular, USA) with different outside diameters of 1.4 mm, 1.7 mm and 2.4 mm. The results show that the developing trends of the compression load with the compression displacement measured by the three radial support property test methods are the same, but normalized radial force values are quite different. The planar compression method is more suitable for comparing the radial support properties of stents with different diameters and structures. Compression rate has no obvious effect on the testing results of both the radial support property and flexibility. Compression circumferential position has a great impact on testing radial support property with the planar or V-groove compression methods and testing flexibility with three-point bending method. The radial support properties of all the three self-designed stents are improved at a certain degree compared to that of the BVS stent. The study has better guide significance and reference value for testing mechanical properties of vascular stents.


Subject(s)
Absorbable Implants , Mechanical Phenomena , Polymers , Prosthesis Design , Stents , Stress, Mechanical
17.
Article in Chinese | WPRIM | ID: wpr-799995

ABSTRACT

The open access of electronic health record(EHR) is an important link to promote the construction and application of EHR. Starting with the definition of open data of EHR, the authors discussed the content and form of open data of electronic health records, summarized the advanced experiences of countries with higher openness of EHR in the United States, the United Kingdom and Denmark. This paper combined the current situation that the opening of EHR in China is limited within data access instead of data utilization. It also pointed out such problems in the opening of EHR in China, as unclear data ownership, weak health awareness of residents and lack of unified construction standards.From the point of view of the state and medical institutions, this paper put forward a series of policy suggestions, such as issuing standards to standardize the opening of EHR, implementing patient real-name certification, realizing the overall opening of regional platforms, and linking the scope of application and supervision with the content of data opening.

18.
Article in Chinese | WPRIM | ID: wpr-710701

ABSTRACT

Objective To investigate the effects of dexmedetomidine on myocardial injury in adult liver transplantation.Methods Sixty patients of both sexes,aged 42-63 years,with body mass index (BMI) of 18-25 kg/m2,scheduled for adult liver transplantation,were randomly divided into two groups (n =30 each) using a random number table:control group (group C) and dexrnedetomidine group (group D).In group D,dexmedetomidine was infused intravenously at a dose of 0.5μg/kg over 12 min immediately before skin incision,followed by 0.5 μg· kg-1· h-1 infusion until the end of surgery.The equal volume of normal saline was given in group C.Immediately before skin incision (baseline,T0),at 30 min of anhepatic phase (T1),at 30 min of neohepatic phase (T2),at the end of surgery (T3),at 4 h after surgery (T4) and at 24 h after surgery (Ts),blood samples were obtained from central vein for tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6),high mobility family protein B1 (HMGB1),cardiac troponin I (cTnI),myoblobin (Myo) and creatine kinase isoenzyme (CK-MB).The occurrence of adverse cardiac events during and afer surgery for all patients was recorded.Results Compared with the baseline value at T0,the serum levels of TNF-α and IL-6 were increased at T1-5;The levels of HMGB1,cTnI,Myo and CK-MB were increased at T2-5 in both groups (P<0.05).Compared with group C,the serum concentrations of TNF-α and IL-6 were reduced at T1-5 in group D (P<0.05).the serum concentrations of HMGB1,cTnI,Myo and CK-MB were reduced at T2-5 in group D (P < 0.05).The occurrence of myocardial ischemia and supraventricular tachycardia was reduced at T1-5 in group D (P<0.05).Conclusion Dexmedetomidine can attenuate the myocardial injury of adult liver transplantation.

19.
Article in Chinese | WPRIM | ID: wpr-709879

ABSTRACT

Objective To evaluate the role of sirtuin 3 ( SIRT3)∕forkhead box O3α ( FOXO3α) signaling pathway in dexmedetomidine-induced reduction of hepatic ischemia-reperfusion ( I∕R) injury in mice. Methods Forty clean-grade C57BL∕6 mice of both sexes, aged 2 weeks, weighing 6-8 g, were di-vided into 4 groups (n=10 each) using a random number table method: sham operation group (group S), hepatic I∕R group ( group I∕R), dexmedetomidine group ( group D) and SIRT3 inhibitor 3-TYP plus dexmedetomidine group (group T+D). Portal vein and hepatic artery supplying left and middle lobes of the liver and biliary tract were clamped resulting in ischemia of 70% of the liver in anesthetized rats. Normal sa-line 0. 25 ml was intraperitoneally injected at 1 h before establishing model, and 30 min later dexmedetomi-dine 50 μg∕kg (diluted to 0. 25 ml in normal saline) was intraperitoneally injected in group D. In group T+D, 3-TYP 5 mg∕kg (diluted to 0. 25 ml in normal saline) was intraperitoneally injected at 1 h before estab-lishing model, and 30 min later dexmedetomidine 50 μg∕kg (diluted to 0. 25 ml in normal saline) was in-traperitoneally injected. Mice were selected at 6 h after reperfusion, blood samples were obtained through eyeball, and the mice were then sacrificed and kidneys were removed for determination of the serum concen-trations of creatinine (Cr) and blood urea nitrogen (BUN), cell apoptosis (by TUNEL), malondialdehyde (MDA) content (using thiobarbituric acid method), superoxide dismutase (SOD) activity (by xanthine oxidase method), and acetylation of FOXO3α in renal tissues (by using immunoprecipitation) and for ex-amination of the pathologic changes. The damage to renal tubules was scored. Apoptosis index ( AI) was calculated. Results Compared with group S, the renal tubular damage score and AI were significantly in-creased, serum concentrations of Cr and BUN were increased, the content of MDA was increased, the ac-tivity of SOD was decreased, and the acetylation of FOXO3α was decreased in I∕R, D and T+D groups ( P<0. 05). Compared with group I∕R, the renal tubular damage score and AI were significantly decreased, serum concentrations of Cr and BUN were decreased, the content of MDA was decreased, the activity of SOD was increased, and the acetylation of FOXO3α was decreased in group D (P<0. 05), and no signifi-cant change was found in the parameters mentioned above in group T+D (P>0. 05). Compared with group D, the renal tubular damage score and AI were significantly increased, serum concentrations of Cr and BUN were increased, the content of MDA was increased, the activity of SOD was decreased, and the acetylation of FOXO3α was decreased in group T+D ( P<0. 05). Conclusion Activation of SIRT3∕FOXO3α signaling pathway is involved in dexmedetomidine-induced reduction of hepatic I∕R injury in mice.

20.
Article in Chinese | WPRIM | ID: wpr-709870

ABSTRACT

Objective To evaluate the effect of dexmedetomidine on renal function in pediatric pa-tients undergoing living donor liver transplantation. Methods Sixty pediatric patients of both sexes with congenital biliary atresia, aged 5-15 months, weighing 5. 5-10. 0 kg, of American Society of Anesthesiol-ogists physical status Ⅱ or Ⅲ, scheduled for elective living donor liver transplantation, were randomly di-vided into either control group (group C) or dexmedetomidine group ( group D) with 30 patients in each group. The infants were tracheally or nasotracheally intubated and mechanically ventilated after induction of anesthesia. Dexmedetomidine was intravenously infused in a loading dose of 1 μg∕kg for 10 min, followed by a continuous infusion of 0. 3 μg·kg-1·h-1until the end of operation in group D. While the equal vol-ume of normal saline was given instead of dexmedetomidine in group C. At skin incision (T1), at 30 min of anhepatic phase (T2), at 1 h of neohepatic phase ( T3), immediately after peritoneum closure ( T4) and at 24 h after operation (T5), blood samples from the central vein and urine specimens were collected for determination of creatinine and blood urea nitrogen (BUN) concentrations in serum (by colorimetric assay) and β2-microglobulin (β2-MG) concentrations in serum and urine (using immunoturbidimetric method), and concentrations of tumor necrosis factor-α, interleukin-6 (IL-6) and IL-18 in serum (by enzyme-linked immunosorbent assay). The urine volume was recorded during surgery. Results Compared with group C, the urine volume was significantly increased, and the serum creatinine, BUN and β2-MG concentrations and urine β2-MG concentrations at T3-5, serum BUN concentrations at T4,5, and serum tumor necrosis fac-tor-α, IL-6 and IL-18 concentrations at T2-5were decreased in group D (P<0. 05). Conclusion Dexme-detomidine can improve renal function in pediatric patients undergoing living donor liver transplantation.

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