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1.
Chinese Journal of Stomatology ; (12): 109-113, 2016.
Article in Chinese | WPRIM | ID: wpr-259434

ABSTRACT

<p><b>OBJECTIVE</b>To explore biomimetic mineralization of polyelectrolyte multilayer films (PEM) of gene-loaded lipopolysaccharide-amine nanopolymersomes/hyaluronic acid self assembled on titanium surface.</p><p><b>METHODS</b>Via lay-by-layer self assembly technology, PEM were constructed on titanium or quartz surface using bone morphogenetic protein-2(BMP-2) plasmid-loaded lipopolysaccharide-amine nanopolymersomes(pLNP) as a polycation, and hyaluronic acid(HA) as a polyanion. The constructed PEM were defined as substrate-pLNP-(HA-pLNP)n, where a successive deposition of HA and pLNP on substrate surface was defined as one assembly cycle, and n was the cycle number. Biomimetic mineralization on surfaces of Ti-pLNP-(HA-pLNP)4(Group A, with outermost layer of pLNP), Ti-pLNP-(HA-pLNP)4.5(Group B, with outermost layer of HA), blank control(polished titanium, Ti) and alkaline-heat treated titanium(Ti-OH) were investigated. The biomimetic mineralization was analyzed by observing the topography under field-emisssion electron microscopy(FE-SEM), characterizing the surface chemical structure and components via X-ray diffractometer(XRD) and X-ray energy disperse spectroscopy(EDS).</p><p><b>RESULTS</b>For experiment groups, XRD analysis showed that the diffraction peak of hydroxyapatite appeared, and its intensity was higher than that for Ti group. FE-SEM images showed that its surface was homogeneously covered by discrete agglomerate of big particles. EDS spectra showed that the percentage of Ca and P were 77.24% and 64.23%, and these were much higher than those in Ti group.</p><p><b>CONCLUSIONS</b>The surface of Ti-pLNP-(HA-pLNP)n is favorable for in vitro biomimetic mineralization.</p>


Subject(s)
Amines , Chemistry , Biomimetic Materials , Chemistry , Bone Morphogenetic Protein 2 , Durapatite , Chemistry , Hyaluronic Acid , Chemistry , Lipopolysaccharides , Nanocomposites , Chemistry , Plasmids , Surface Properties , Titanium , Chemistry
2.
Chinese Journal of Stomatology ; (12): 166-171, 2016.
Article in Chinese | WPRIM | ID: wpr-259423

ABSTRACT

<p><b>OBJECTIVE</b>To provide a theoretical basis for surface modification of titanium implants, the effects of the stiffness of polyelectrolyte multilayer films on titanium surface on bacterium adhesion was explored.</p><p><b>METHODS</b>Via layer-by-layer technique, catechol functionalized polyelectrolyte multilayer film (cPEM) was constructed on titanium surface by using catechol functionalized hyaluronic acid (cHA) and lipopolysaccharide-amine nanopolymersomes (NP). The stiffness of cPEM was controlled by adjusting the catechol substitution degree of cHA (5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%). Titanium samples covered with cPEM were selected as test group, and the cPEM was constructed with the lowest, medium and highest stiffness. The polished titanium was used as a control. The surface topography of titanium before and after film construction was observed by scanning electron microscopy (SEM). At 1 and 24 h after incubation, the adhesion and clonal formation of Streptococcus mutans (S. mutans) on different titanium surfaces were quantified, and their morphology and survival status were observed by SEM and laser scanning confocal microscope (LSCM).</p><p><b>RESULTS</b>When the catechol grafting ratio was 5%, 30% and 70%, the lowest, medium and highest cPEM stiffness were obtained, and the cPEM stiffness were (10.69±4.54) GPa(cPEM-L), (20.99± 5.81) GPa (cPEM-M) and (32.57±6.93) GPa (cPEM-H) respectively, and the stiffness of polished titanium was (107.12±8.68) GPa (P<0.05). SEM observation showed that after cPEM coating, the titanium surface became smoother. After incubation for 1 and 24 h, the amount of adhesion and clonal formation of S. mutans on cPEM were higher than those on control titanium, and the difference was statistically significant (P<0.05). SEM images showed that for 1 h incubation, softer surfaces were beneficial for S. mutans adhering and agglomerating, while this difference nearly disappeared at 24 h. Observation under LSCM revealed that most of bacteria were alive on titanium disks at 1 h, and their amount decreased with the increase of stiffness. At 24 h, the living/dead bacterium ratios on cPEM-L and control titanium was higher than that on cPEM-M and cPEM-H, and cPEM-L surface was dominated by living bacteria, while stiffer cPEM-M and cPEM-H had more dead bacteria than living bacteria.</p><p><b>CONCLUSIONS</b>Increasing the stiffness of polyelectrolyte films on titanium limits the adhesion of S. mutans. As an independent factor, stiffness influences the bacterium adhesion.</p>


Subject(s)
Bacterial Adhesion , Catechols , Elasticity , Hyaluronic Acid , Lipopolysaccharides , Microscopy, Confocal , Microscopy, Electron, Scanning , Nanoparticles , Polymers , Chemistry , Streptococcus mutans , Physiology , Surface Properties , Time Factors , Titanium , Chemistry
3.
Journal of Practical Stomatology ; (6): 748-752, 2015.
Article in Chinese | WPRIM | ID: wpr-479712

ABSTRACT

Objective:To study the influence of all-trans retinoic acid (atRA)on craniomaxillofacial development of C57 mice. Methods:Pregnant C57BL mice were divided into 4 groups(n =5)at gestation day (GD)1 0.Mice in three atRA-induction groups were given atRA of 60,80 and 1 00 mg/kg,respectively.The mice in control group were given the equivalent volume of corn oil.All pregnant mice were sacrificed at GD1 9 and the embryos were collected.Stereo microscope was used to observe the craniomaxillofacial morphology.Standardized radiographs were taken and cephalometric analysis was performed.Results:The embryonic body length and body mass of control group surpassed those of 80 and 1 00 mg/kg atRA groups(P <0.05,P <0.01 ).atRA induced craniomaxillofacial malformations and maldevelopment.The mice induced by atRA exhibited a shorter mandibular body and more retrusive position of max-illary and mandibular(∠NAK and ∠NBD)when compared with their norm(P <0.01 ).Significant decrease in craniofacial length (Op-Rh)was observed in all atRA-induced groups(P <0.01 ).Decreases in cranial vault height(Fp-Os)and cranial vault length(Pa-Na)dimensions were observed in 80 and 1 00 mg/kg atRA groups(P <0.05,P <0.01 ).Conclusion:Exogenous atRA dose-depend-ently induces retardation of craniomaxillofacial morphology in embryo of C57BL mice by inhibition of the sagital and vertical dimension development of the bone.

4.
West China Journal of Stomatology ; (6): 377-382, 2015.
Article in Chinese | WPRIM | ID: wpr-261068

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of all-trans retinoic acid (atRA) on the function of bone morphogenetic protein receptor 2 (BMPR2) expression in embryonic palate.</p><p><b>METHODS</b>Cleft palate mice model was established by atRA. On gestation day (GD) 15 and GD 17, the pregnant mice were killed to obtain the embryos from the uteri. The embryonic palates were stained with hematoxylin-eosin, and the remaining sections were used for the immunohistochemistry of BMPR2 detection. Reverse transcription-polymerase chain reaction was performed to detect the expression levels of Bmpr2 mRNA.</p><p><b>RESULTS</b>In the atRA-treated group, short extensions and failure to fuse with each other were observed. The positive expression of BMPR2 was detected in developing palatal process from GD 15 to GD 17 in the control group. Compared with those of the control group, BMPR2 protein and Bmpr2 mRNA decreased in the atRA-treated group (P<0.05).</p><p><b>CONCLUSION</b>The treatment of pregnant mice with retinoic acid produces small palatal shelves in their fetuses and down-regulates BMPR2 expressions.</p>


Subject(s)
Animals , Female , Mice , Pregnancy , Bone Morphogenetic Protein 2 , Cleft Palate , Disease Models, Animal , Down-Regulation , RNA, Messenger , Tretinoin
5.
Chinese Journal of Pharmacology and Toxicology ; (6): 836-841, 2015.
Article in Chinese | WPRIM | ID: wpr-477321

ABSTRACT

OBJECTIVE To investigate the effect and related mechanism of all ̄trans retinoic acid (atRA) exposure on osteogenic differentiation of mouse embryonic palate masenchymal cells MEPM. METHODS MEPM were cultured in osteogenic medium (OM) with atRA 0.1 and 1.0 μmol??L-1 for 1, 3,5, 7 and 9 d. MTT assay was performed to measure the cell viability. The alkaline phosphatase (ALP) activity was measured by chemical colorimetry. The cells were stained using the Von ̄Kossa technique to detect the formation of mineralization nodules after 21 d of culture. RT ̄PCR was performed to determine expression Runx2, osteopontin, bone morphogenetic protein receptor ( Bmpr) 1b, Bmpr2 and Smad5 mRNA. RESULTS The result of MTT on 9 d showed that, compared with normal control group, the cell viability of OM, OM+atRA 0.1 and 1.0 μmol??L-1 groups decreased significantly(P<0.01). Compared with normal control group, ALP activity of OM group increased significantly(P<0.05), while the ALP activity of OM+atRA 0.1 and 1.0 μmol??L-1 groups was lower than OM group(P<0.05). On 21 d, the Von ̄Kossa stai ̄ning results showed that the percentage of mineralization nodules formation of OM+atRA 1.0 μmol??L-1 group was (3.65±1.24)%, which was significantly lower than that of OM group(10.33±2.29)%(P<0. 05). On 9 d, the relative Run expression of OM group was the highest one in the four groups, while at ̄RA 1.0 μmol??L-1 treatment negatively regulated 20% in comparsion with OM group(P<0.05). Compared with normal control group, the mRNA expression of osteopontin of OM, OM+atRA 0.1 and 1.0 μmol??L-1 groups increased significantly(P<0.05); BDNF mRNA expression of OM group was 2.6 ̄fold to normal control group, while that of OM+atRA 1.0 μmol??L-1 group was 33% to OM group(P<0.05) . The level of Smad5 mRNA of OM+atRA 1.0 μmol??L-1 group was significantly lower than that of OM group(P<0.05). CONCLUSION atRA Might inhibit osteogenic differentiation of MEPM by down ̄regulated the expression of Bmpr1b.

6.
Chinese Journal of Stomatology ; (12): 758-762, 2014.
Article in Chinese | WPRIM | ID: wpr-360484

ABSTRACT

<p><b>OBJECTIVE</b>To provide a basis for surface modification of polyelectrolyte multilayer films (PEM) on implants by exploring the effects of immobilization of PEM on titanium surfaces on their cell biological effects.</p><p><b>METHODS</b>By using plasmid of bone morphogenetic protein-2 (pBMP-2)-loaded lipopolysaccharide-amine nanopolymersomes (pLNP) as cationic polyelectrolytes and hyaluronic acid (HA) as anionic polyelectrolytes. PEM were constructed on alkaline-heat treated titanium surfaces via layer by layer self-assembly(LbL) technique. A successive deposition of HA and pLNP on titanium surfaces was defined as one cycle of assembly. The morphology of titanium surface before and after assembly treatment was observed by scanning electron microscopy (SEM). The ultraviolet (UV) spectra and surface contact angles of PEM with different self-assembly cycles were measured. The adhesion and proliferation of mesenchymal stem cell (BMSC) on surfaces of group A (4 cycles of assembly, with outermost layer of pLNP), group B (4.5 cycles of assembly, with outermost layer of HA), blank control (polished titanium, Ti group) and alkaline-heat treated titanium (Ti-OH group) were investigated. Cell differentiation indexed by alkaline phosphatase activity (ALP) and in situ transfection of BMSC on surfaces of group A, Ti, negative control [4 cycles of assembly without pBMP- 2] were evaluated.</p><p><b>RESULTS</b>Self assembly of PEM made the titanium surface become relatively smooth. DNA absorption peaked appear at 260 nm in UV spectra, and the absorption intensity increased with assembly, suggesting the successful construction of PEM. After alkali- heat treatment, the surface contact angle of titanium decreased from (62.6±4.9) ° to (8.1±2.2) °. During LbL, with alternately introducing pLNP and HA, the contact angle increased in a jagged mode at a gradually decreased rate, which further proved the success of assembly. Cell adhesion for group A at 0.5 and 1 h was 0.415±0.085 and 0.426±0.048, which was significantly higher than those for group B (0.299±0.012, 0.355±0.022), Ti-OH group (0.225±0.007, 0.260±0.010) and Ti group (0.302±0.056, 0.339±0.028) (P < 0.01). Cell proliferation for group A and B at 3, 5 and 7 d were significantly higher than those for Ti and Ti-OH group (P < 0.01). ALP in group A at day 7 was 261±58, which was significantly higher than those in group B and Ti group. Group A had in situ transfection capability for BMSC.</p><p><b>CONCLUSIONS</b>PEM with good cell biological effects can be constructed successfully on titanium surfaces using gene- loaded lipopolysaccharide- amine nanopolymersomes and hyaluronic acid.</p>


Subject(s)
Alkaline Phosphatase , Metabolism , Bone Morphogenetic Protein 2 , Genetics , Cell Adhesion , Cell Differentiation , Cell Proliferation , DNA , Hyaluronic Acid , Chemistry , Pharmacology , Mesenchymal Stem Cells , Physiology , Microscopy, Electron, Scanning , Nanoparticles , Chemistry , Plasmids , Polysaccharides, Bacterial , Pharmacology , Prostheses and Implants , Surface Properties , Titanium , Chemistry , Transfection
7.
West China Journal of Stomatology ; (6): 303-309, 2013.
Article in Chinese | WPRIM | ID: wpr-336333

ABSTRACT

<p><b>OBJECTIVE</b>To explore the expression of Notch signaling receptors Notchl, Notch3 and its ligand Jaggedl, Jagged2 in tongue squamous cell carcinoma (TSCC).</p><p><b>METHODS</b>mRNA and protein expression levels of tissue samples from 74 cases of tongue cancer patients and human tongue cell line Cal-27 were detected by reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry and Western blot. Its relationship with cell proliferation and clinical pathology was analyzed.</p><p><b>RESULTS</b>mRNA and protein expression were detected in tongue cancer tissues, adjacent tissues and cell lines. Notchl and Notch3 protein expression in tongue cancer was higher than the adjacent tissues. Jaggedl and Jagged2 protein expression in tongue cancer and adjacent tissues had no difference. Notchl and Notch3 protein had correlation with tongue cancer clinical staging. Pathway protein expression had no correlation with pathological grade, age, gender. Notchl protein expression in lymph node metastasis-positive cases was higher than in lymph node metastasis-negative cases. The expression of Notch3 and Jagged2 had correlation. Jaggedl expression grade in metastasis-positive cases was higher than in negative cases.</p><p><b>CONCLUSION</b>Notch signaling molecules have active expression in TSCC and may play important roles in tongue cancer development.</p>


Subject(s)
Humans , Blotting, Western , Carcinoma, Squamous Cell , Cell Line , Cell Proliferation , Immunohistochemistry , Lymphatic Metastasis , Neoplasm Staging , RNA, Messenger , Receptors, Notch , Tongue , Tongue Neoplasms
8.
Chinese Journal of Medical Aesthetics and Cosmetology ; (6): 121-124, 2012.
Article in Chinese | WPRIM | ID: wpr-428635

ABSTRACT

ObjectiveTo investigate an optimal treatment of localized low flow type venous malformation located in the oral and maxillofacial region.MethodsFifty seven patients with localized low-flow venous malformation were treated by intralesional injection of PYM.The injections was repeated at an interval of 10 to 14 days,but not more than 3-5 sessions within a therapeutic period.If necessary,the secondary therapeutic period was performed 1 month later.The general and local adverse responses and the appearance improvement were recorded.The clinical outcomes and aesthetic effects were assessed with a follow-up of 1 to 3 years.ResultsAfter 3-8 injections,complete clinical resolutions were achieved in 52 patients.The deformity disappeared thoroughly.The surface skin and the appearance of lesions showed normal.4 patients received completed lesion control and showed nor mal skin or muco but a little hypertrophy tissue.One venous malformation reduced 2/3 volume after 8injections but improved slowly.The therapeutic time seemed to be related with the size of lesion.Diameter less than 3 cm could be usually cured within 1 treatment period.No ulcerations or scars were presented in injection regions.The function of nerves in oral and maxillofacial region remained normality in all patients.The systematic complication included transient pyrexia and poor appetite appeared in several cases.No allergy was found.No clinical recurrence was observed during the follow-up.ConclusionsTreatment of localized low-flow venous malformations in oral and maxillofacial region with PYM sclerotherapy reveals a high rate of complete clinical resolution,a fair cosmetic and function result,and does not damage facial nerves or form local scars.And it might be regard as an optimal therapeutic method to localized low-flow venous malformations.

9.
Chinese Journal of Medical Aesthetics and Cosmetology ; (6): 370-372, 2010.
Article in Chinese | WPRIM | ID: wpr-382899

ABSTRACT

Objective To evaluate the clinical efficacy of pingyangmycin (PYM) injection on infantile hemangioma located in the parotid gland region. Methods Twelve patients were treated by intralesional injection of PYM. When necessary, the injections were repeated at an interval of one week, but not more than 3-4 sessions within a therapeutic period. Normally, the secondary therapeutic period was performed 1 month later. The general and local adverse responses were recorded and the clinical outcomes were assessed with a follow-up of 1 to 3 years. Results Complete clinical resolutions were achieved in 10 patients. 2 patients received one injection, 3 patients received 2 injections, 3 patients received 3 injections, and 2 patients received 4 injections. The remaining 2 patients with partial resolution received 6 and 7 injections respectively. No clinical recurrence was observed during the follow-up of 1 to 3 years. No ulcerations or postoperative sears in injection regions were presented. The function of facial nerve was remained normality in all patients. The systematic side effects included transient pyrexia and poor appetite appeared in partial patients. No allergy cases were found. Conclusion Treatment of infantile hemangioma located in parotid gland region with PYM injection reveals a high rate of complete clinical resolution, with fair cosmetic results and short treatment time, and it does not damage the facial nerve or form local scar.The treatment time of PYM injection seems to be positively related to size of the lesions.

10.
Chinese Medical Journal ; (24): 811-818, 2003.
Article in English | WPRIM | ID: wpr-294225

ABSTRACT

<p><b>OBJECTIVE</b>To describe a hospital outbreak of severe acute respiratory syndrome (SARS) and summarize its clinical features and therapeutic approaches.</p><p><b>METHODS</b>The outbreak started with a SARS patient from the community, and a total of 96 people (76 women and 20 men, mean age (29.5 +/- 10.3) years, 93.8% of whom were health care workers) who had exposure to this source patient became infected in a short time. Clinical data in this cohort were collected prospectively as they were identified.</p><p><b>RESULTS</b>(1) The incubation period ranged from 1 to 20 (mean: 5.9 +/- 3.5) days. The duration of hospitalization was (17.2 +/- 8.0) days. (2) The initial temperature was (38.3 +/- 0.6) degrees C, while the highest was (39.2 +/- 0.6) degrees C (P < 0.001), with fever duration of (9.0 +/- 4.2) days. (3) Other most common symptoms included fatigue (93.8%), cough (85.4%), mild sputum production (66.7%), chills (55.2%), headache (39.6%), general malaise (35.4%) and myalgia (21.9%). (4) The radiographic changes were predominantly bilateral in the middle or lower lung zones. The number of affected lung fields was 1.2 +/- 0.8 on presentation, which increased to 2.9 +/- 1.4 after admission (P < 0.001). The interval from the beginning of fever to the onset of abnormal chest radiographs was (3.5 +/- 2.3) days, which increased in size, extent, and severity to the maximum (6.7 +/- 3.5) days later. The time before the lung opacities were basically absorbed was (14.9 +/- 7.8) days. (5) Leukopenia was observed in 67.7% of this cohort. The time between the onset of fever and leukopenia was (4.4 +/- 2.3) days, with the lowest white blood cell count of (2.80 +/- 0.72) x 10(9)/L. (6) The lowest arterial oxygen saturation was (94.8 +/- 3.1)% with supplementary oxygen. (7) Antibiotical therapies included tetracyclines (91.0%), aminoglycosides (83.3%), quinolones (79.2%); 18.8% of the patients received a combination of tetracyclines and aminoglycosides, while 11.5% received a combination of tetracyclines and quinolones, and 63.5% received a combination of tetracyclines, aminoglycosides and quinolones. Vancomycin was used in 13.5% of the patients. (8) 68.8% of the patients were treated with methylprednisolones for a mean interval of (4.9 +/- 2.4) days. The initial dose was (67.3 +/- 28.2) mg/d and the maximal dose was (82.4 +/- 30.5) mg/d. (9) Human gamma-globulin, interferon-alpha, antiviral drugs (oral ribavirin or oseltamivir) were used respectively in 68.6%, 46.9% and 92.7% of the patients. (10) Ninety-five patients (99.0%) had a complete clinical recovery, and only 1 patient (1.0%) died.</p><p><b>CONCLUSIONS</b>SARS appears to be quickly infectious and potentially lethal among health care workers, characterized by acute onset and rapid progression, and mostly bilateral lung involvement on chest radiographs. Proper administration of glucocorticosteroids seems to be of some benefits. Antibiotics, human gamma-globulin, interferon-alpha, and antiviral drugs, although empirically, might be useful to shorten the clinical course.</p>


Subject(s)
Adult , Female , Humans , Male , China , Epidemiology , Cross Infection , Diagnosis , Epidemiology , Therapeutics , Disease Outbreaks , Severe Acute Respiratory Syndrome , Diagnosis , Epidemiology , Therapeutics
11.
Chinese Medical Journal ; (24): 1265-1266, 2003.
Article in English | WPRIM | ID: wpr-311703

ABSTRACT

<p><b>OBJECTIVE</b>To explore digestive system manifestations in patients with severe acute respiratory syndrome (SARS).</p><p><b>METHOD</b>The clinical data of 96 cases with SARS admitted into our hospital from February 6, 2003 to March 28, 2003 were retrospectively analyzed.</p><p><b>RESULTS</b>Among the 96 cases, 26 cases (27%) had diarrhea, 17 (18%) had nausea, 6 (6%) had vomiting, 16 (17%) had bellyache, and 8 (8%) had ALT elevation.</p><p><b>CONCLUSIONS</b>Patients with SARS may have digestive system manifestations; diarrhea is the most common symptom.</p>


Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Diarrhea , Digestive System Diseases , Retrospective Studies , Severe Acute Respiratory Syndrome
12.
West China Journal of Stomatology ; (6): 180-183, 2003.
Article in Chinese | WPRIM | ID: wpr-283631

ABSTRACT

<p><b>OBJECTIVE</b>The purpose of this study was to establish a stable, high-efficient and low-toxic way of transfecting nm23-H1 into Tca8113 line cells, and then to find out whether nm23-H1 could affect the invasion and metastases ability of Tca8113 line cells.</p><p><b>METHODS</b>nm23-H1 was transfected into Tca8113 line cells with Lipofect. The different expressions of nm23-H1 between transfected and non-transfected line cells were detected by the method of immunohistochemistry. The difference of the invasion and metastases ability between transfected and non-transfected line cells was detected by transwell-room and wash techniques. The change of chemo-sensitivity was evaluated by MTT.</p><p><b>RESULTS</b>Using pCMV-NEO-BAM system to keep stable expression of nm23-H1, the significant difference of NDPKA expression between transfected and non-transfected Tca8113 line cells was discovered; The metastases ability of transfected Tca8113 line cells decreased significantly; The chemo-sensitivity of transfected Tca8113 line cells to CDDP increased significantly.</p><p><b>CONCLUSION</b>nm23-H1 can inhibit the metastases of Tca8113 line cells and increase the chemo-sensitivity to CDDP significantly.</p>


Subject(s)
Humans , Antineoplastic Agents , Pharmacology , Carcinoma, Squamous Cell , Genetics , Pathology , Cisplatin , Pharmacology , Drug Resistance, Neoplasm , Gene Expression Regulation, Neoplastic , NM23 Nucleoside Diphosphate Kinases , Neoplasm Metastasis , Nucleoside-Diphosphate Kinase , Proteins , Genetics , Tongue Neoplasms , Genetics , Pathology , Transfection , Tumor Cells, Cultured
13.
Chinese Medical Journal ; (24): 248-252, 2003.
Article in English | WPRIM | ID: wpr-356823

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the therapeutic effect and metabolism of 5-fluorocytosine (5-FC) in human tongue squamous carcinoma cells after treatment with adenovirus-mediated cytosine deaminase (AdCMVCD)/5-FC system.</p><p><b>METHODS</b>Human tongue squamous carcinoma cells (Tca8113 cell line) and its xenografts in BALB/c nude mice were treated with AdCMVCD/5-FC system. The killing effect in vitro and bystander effect were detected by microculture tetrazolium (MTT) assay. Tumor inhibition effect and histopathological changes were observed in vivo. High-performance liquid chromatography (HPLC) was performed to determine the metabolism of 5-FC in vitro and in vivo.</p><p><b>RESULTS</b>AdCMVCD/5-FC system had strong killing effect and bystander effect on Tca8113 cells. Both condition media and cell extracts showed two peaks identified as 5-FC and 5-fluorouracil (5-FU) by HPLC and a time-dependent generation of 5-FU and concomitant time-dependent decreases of 5-FC. Compared to the control groups, mice treated with AdCMVCD/5-FC system demonstrated significant tumor regression (P < 0.001); the tumor doubling time prolonged and inhibition rate was 92.62%. There were substantial tumor necrotic areas and infiltrative lymphocytes around necrotic areas in the AdCMVCD/5-FC treated group under light microscope. There was a significantly low concentration of 5-FC and high concentration of 5-FU in tumor tissue, but only 5-FC was found in blood.</p><p><b>CONCLUSION</b>AdCMVCD/5-FC suicide gene system had significant in vitro and in vivo anti-tumor effect on human tongue squamous cell carcinoma due to convert 5-FC into 5-FU.</p>


Subject(s)
Animals , Female , Humans , Mice , Adenoviridae , Genetics , Carcinoma, Squamous Cell , Pathology , Therapeutics , Cytosine Deaminase , Flucytosine , Metabolism , Therapeutic Uses , Genetic Therapy , Mice, Inbred BALB C , Neoplasm Transplantation , Nucleoside Deaminases , Genetics , Tongue Neoplasms , Pathology , Therapeutics , Transplantation, Heterologous , Tumor Cells, Cultured
14.
Chinese Journal of Stomatology ; (12): 167-169, 2002.
Article in English | WPRIM | ID: wpr-244810

ABSTRACT

<p><b>OBJECTIVE</b>To establish an immortalized ameloblastoma cell line.</p><p><b>METHODS</b>The primary cultured ameloblastoma cells were transfected with pRSV-Tag using Transfect AMINE kit. Tansfected cells were passaged to pass through crisis period and immortalize.</p><p><b>RESULTS</b>Cultured ameloblastoma cells were composed predominantly of closely packed small polygonal cells with epithelial morphology. They had limited life-span of 51 days in vitro. The small polygonal cells were eventually replaced by large flattened cells and subsequently became senescent and dead. On the other side, those tumor cells transfected with SV40Tag could live for a longer time. The majority of them died in crisis period while the survived cells from crisis period gained the ability to proliferate. There was no morphological change in TAM-1 compared with original cultured cells. A cell clone was harvested which was alive and keeping on proliferating after having been subcultured for 25 times. It was named TAM-1. The epithelial origin of TAM-1 was confirmed by strong immunoreactivity for cytokeratin in contrast to negative vimentin expression. It was detected that SV40Tag had been transfected into TAM-1 genesome and expressed continuously by PCR and RT-PCR.</p><p><b>CONCLUSIONS</b>TAM-1 is immortalized ameloblastoma cell line in vitro.</p>


Subject(s)
Female , Humans , Ameloblastoma , Genetics , Metabolism , Pathology , Antigens, Polyomavirus Transforming , Genetics , Cell Division , Genetics , Cell Line, Transformed , Cell Survival , Genetics , Immunohistochemistry , Jaw Neoplasms , Genetics , Metabolism , Pathology , Keratins , Plasmids , Genetics , Time Factors , Transfection , Tumor Cells, Cultured , Vimentin
15.
Journal of Practical Stomatology ; (6)2001.
Article in Chinese | WPRIM | ID: wpr-542473

ABSTRACT

Objective:To investigate the feasibility of modified combined radical neck dissection remaining integrality of mandible in the surgical management of advanced tongue squamous cell carcinoma.Methods:39 cases of advanced tongue squamous cell carcinoma received modified combined radical operation remaining integrality of mandible,7-year follow-up was conducted.Results:Integrality of mandible and the function of mastication were remained in all the cases.1 and 3 years after operation there were 1 case and 9 cases of recurrence respectively.Metastasis was found in 5 cases.The 3-year survival rate was(61.72?5.44)%.Conclusion:The modified combined radical neck dissection remaining integrality of mandible is feasible in the treatment of advanced tongue squamous cell carcinoma.

16.
Chinese Journal of Medical Aesthetics and Cosmetology ; (6)2001.
Article in Chinese | WPRIM | ID: wpr-537141

ABSTRACT

Objective To investigate the aesthetic relations and internal regularities in normal craniofacial stereoscopic architecture. Methods By means of computer aided three dimensional measurement and analysis, a study on morphological features and inter relations of the cranio orbitozygomatic maxilla structures of twenty youths with pretty facial appearance was carried out. Results There were remarkable differences between each craniofacial structure in different sexes, but the ratio indices that represented the inter-relations of different craniofacial structures were closely similar in both sexes. In the group of youths the asymmetry ratios between bilateral craniofacial structures were below 5 % , presenting the perfect symmetry feature. The asymmetry ratios and their standard deviations of the zygomas and angulus mandibulae were relatively large . The results suggested that these two characteristic parts which influence the whole appearance were easily changeable with different individuals. There were close co relations between coronal axis width and sagittal axis protrusion in main structures of craniofacial region. These relationships could sum up in linear regression equations. Conclusion There are internal aesthetic regularities of coordinative ratios and harmonious symmetry in normal three dimensional craniofacial architecture, which determines the pretty appearance of youths. These internal regularities and aesthetic principles should be considered in designing and performing craniofacial aesthetic plastic operation so as to get excellent surgical results.

17.
Journal of Practical Stomatology ; (6)2001.
Article in Chinese | WPRIM | ID: wpr-540695

ABSTRACT

0.05 ),respectively. Conclusions:Modified radical neck dissection with internal jugular vein preservation may have similar therapeutic effect to radical neck dissection.

18.
Chinese Journal of Medical Aesthetics and Cosmetology ; (6)2001.
Article in Chinese | WPRIM | ID: wpr-539964

ABSTRACT

Objective To investigate the mechanisms of enophthalmos and deformities of orbital walls in patients with orbital blowout fracture and to evaluate the reconstructive surgical methods. Methods The features of orbital deformities, the change of orbital volume and the mechanisms of enophthalmos were measured and analyzed by means of 3-D CT technique and 3-D diagnostic computer-assisted system. The reconstructive operations were employed on these 11 cases with orbital blowout fractures to correct deformities and restore orbital volume by applying autogenous bone grafts or hydroxyapatite bone substitutes. Results There were no serious complications such as infection of bone graft, exposure of hydroxypatite implant in all 11 cases, 10 cases got satisfactory appearance of eyelids and orbit, of which thediplopia and enophthalmos were corrected with protrution of eyeball restoring normally. 1 case remained diplopia after surgical correction. Conclusions The enophthalmos is mainly caused by orbital volume enlarging and orbital content herniating into paranasal sinuses. Implanting autogenous grafts and hydroxyapatite substitutes to restore orbital volume are proved to be effective.

19.
Journal of Practical Stomatology ; (6)2000.
Article in Chinese | WPRIM | ID: wpr-670629

ABSTRACT

Objective: To study the therapeutic mechanism of adenovirus mediated cytosine deaminase /5-fluorocytosine (AdCMVCD/ 5-FC) suicide gene system in the treatment of oral squamous carcinoma cells in vitro. Methods: 3H-thymidine ( 3H-TdR) incorporation assay, flow cytometry (FCM), transmission electron microscope and TUNEL (TdT-mediated dUTP Nick End Labeling)assay were used to detected the changes of Tca8113 cells after the treatment with AdCMVCD/5-FC system. Results: After treatment with CD/5-FC system, 3H-TdR incorporation rate of the cells decreased and significantly decreased between different MOI (multiple of infection) at 5-FC 10 -3 mol/L (P0.05); many apoptotic cells were found under transmission electron microscope and the positive rate of apoptotic cells increased from (4.40?0.87)% (before treatment) to (15.80?1.55)% (P

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