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1.
Acta Pharmaceutica Sinica B ; (6): 3966-3982, 2021.
Article in English | WPRIM | ID: wpr-922453

ABSTRACT

Mitochondria as a signaling platform play crucial roles in deciding cell fate. Many classic anticancer agents are known to trigger cell death through induction of mitochondrial damage. Mitophagy, one selective autophagy, is the key mitochondrial quality control that effectively removes damaged mitochondria. However, the precise roles of mitophagy in tumorigenesis and anticancer agent treatment remain largely unclear. Here, we examined the functional implication of mitophagy in the anticancer properties of magnolol, a natural product isolated from herbal

2.
Acta Pharmaceutica Sinica ; (12): 1466-1477, 2020.
Article in Chinese | WPRIM | ID: wpr-823303

ABSTRACT

Snow lotus is a medicinal plant with a wide range of pharmacological activities. It has been used to treat rheumatoid arthritis, cough with cold, stomach ache, dysmenorrhea, and altitude sickness in traditional medicine. This review summarizes the bioactive components in six species of snow lotus including flavonoids, lignans, phenolic compounds, phenylpropanoids, and sesquiterpenes present in Saussurea involucrate (SI), Saussurea obvallata (SO), Saussurea laniceps (SL), Saussurea medusa (SM), Saussurea stella (SS) and Saussurea tridactyla (ST). We review the pharmacological and related molecular mechanisms by which these components exert antineoplastic, anti-inflammatory, and antioxidant effects and promote lipid catabolism, and provide a reference for the future study of the traditional Chinese medicinal chemistry and pharmacological activities of snow lotus.

3.
Article in English | WPRIM | ID: wpr-812547

ABSTRACT

Boiling processing is commonly used in post-harvest handling of White Paeony Root (WPR), in order to whiten the herbal materials and preserve the bright color, since such WPR is empirically considered to possess a higher quality. The present study was designed to investigate whether and how the boiling processing affects overall quality of WPR. First, an ultra-high performance liquid chromatography quadrupole/time-of-flight mass spectrometry-based metabolomics approach coupled with multivariate statistical analysis was developed to compare the holistic quality of boiled and un-boiled WPR samples. Second, ten major components in WPR samples boiled for different durations were quantitatively determined using high performance liquid chromatography to further explore the effects of boiling time on the holistic quality of WPR, meanwhile the appearance of the processed herbal materials was observed. The results suggested that the boiling processing conspicuously affected the holistic quality of WPR by simultaneously and inconsistently altering the chemical compositions and that short-time boiling processing between 2 and 10 min could both make the WPR bright-colored and improve the contents of major bioactive components, which were not achieved either without boiling or with prolonged boiling. In conclusion, short-term boiling (2-10 min) is recommended for post-harvest handling of WPR.


Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Reference Standards , Hot Temperature , Mass Spectrometry , Methods , Paeonia , Chemistry , Plant Roots , Chemistry , Technology, Pharmaceutical , Water
4.
Protein & Cell ; (12): 699-713, 2016.
Article in English | WPRIM | ID: wpr-757387

ABSTRACT

Mitochondria play a key role in various cell processes including ATP production, Ca homeostasis, reactive oxygen species (ROS) generation, and apoptosis. The selective removal of impaired mitochondria by autophagosome is known as mitophagy. Cerebral ischemia is a common form of stroke caused by insufficient blood supply to the brain. Emerging evidence suggests that mitophagy plays important roles in the pathophysiological process of cerebral ischemia. This review focuses on the relationship between ischemic brain injury and mitophagy. Based on the latest research, it describes how the signaling pathways of mitophagy appear to be involved in cerebral ischemia.


Subject(s)
Animals , Brain Ischemia , Metabolism , Pathology , Humans , Mitophagy , Reactive Oxygen Species , Metabolism , Stroke , Metabolism , Pathology
5.
Article in Chinese | WPRIM | ID: wpr-230037

ABSTRACT

To improve the solubility and antitumor activity of ampelopsin, ampelopsin-loaded nanomicelles from the mixture of pluronic F127 and D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS1000) were prepared by film-thin hydration method, in order to optimize the process conditions and physicochemical properties. The antitumor activities against MCF-7 cells between ampelopsin and nanomicelles were compared by MTT method, respectively. The results showed that the optimal nanomicelles were round with the nanometric size of (22.6±0.5) nm, encapsulation efficiency rate of (80.42±1.13)%, and drug-loading rate of (4.41±0.26)%. The solubility of ampelopsin in mixed nanomicelles significantly increased by 16 times. In different release media, the mixed nanomicelles could release more than 90% of drug in 8 h, and showed stronger cytotoxicity and inhibition against MCF-7 cells (P<0.01). The mixed nanomicelles can be used as new drug delivery system of ampelopsin.

6.
Article in Chinese | WPRIM | ID: wpr-237701

ABSTRACT

Chinese medicinal authentication is fundamental for the standardization and globalization of Chinese medicine. The discipline of authentication addresses difficult issues that have remained unresolved for thousands of years, and is essential for preserving safety. Chinese medicinal authentication has both scientific and traditional cultural connotations; the use of scientific methods to elucidate traditional experience-based differentiation carries the legacy of Chinese medicine forward, and offers immediate practical significance and long-term scientific value. In this paper, a path of inheritance and innovation is explored through the scientific exposition of Chinese medicinal authentication, featuring a review of specialized publications, the establishment of a Chinese medicine specimen center and Chinese medicinal image databases, the expansion of authentication technologies, and the formation of a cultural project dedicated to the Compedium of Materia Medica.


Subject(s)
Drug Contamination , Drugs, Chinese Herbal , Chemistry , Reference Standards , Humans , Materia Medica , Chemistry , Reference Standards , Medicine, Chinese Traditional , Reference Standards , Reference Standards
7.
Article in Chinese | WPRIM | ID: wpr-284790

ABSTRACT

This study aimed to simultaneously determine the contents of astilbin and engeletin in dong medicine "sunl gaems" of Guizhou origin by quantitative analysis of multi-components by single marker (QAMS), with astilbin as the internal standard substance. On UPLC and HPLC chromatograms, different models of instruments were used to investigate relative correction factors (RCF), in order to discuss the interoperability of RCFs established in different chromatographic systems. The engeletin content was calculated based on the established RCFs and compared by the one point external standard method and the external standard working curve method, in order to verify the accuracy of QAMS. According to the result, in different chromatograms, the ratios between RCF and retention time of engeletin and astilbin had a good reproducibility, with RSD between 2.0% and 1.8%, both were less than 3%. The relative differences among results of QAMS, the external standard working curve method of dong medicine "sunl gaems" ranged between 1.6% and 3.9%, with RSD between 2.02%-0.80% in line with relevant requirements and Pearson correlation coefficient at 0.9998 (P <0.01). The findings showed that QAMS was an accurate, reliable and highly reproducible method to determine the contents of astilbin and engeletin in dong medicine "sunl gaems" of Guizhou origin and so could be used to control the inherent quality of the herb.


Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Flavonols , Glycosides
8.
Article in Chinese | WPRIM | ID: wpr-318630

ABSTRACT

There are two types of decoction of Smilax glabra due to its reddish brown or off-white colored cross section. These two kinds of decoction were found that they have large difference in anti-inflammatory effects and chemical constituents in the preliminary experiments. Comparing and analyzing the content of total tannin in these two kinds of decoction of S. glabra from 28 areas by UV-Vis spectrophotometry were first used to provide some experimental and theoretical development and utilization of this medicinal resource and quality control. Also, the sample recovery test required in Chinese Pharmacopoeia was improved by adding tannic acid instead of gallic acid to samples.


Subject(s)
Geography , Pigmentation , Reproducibility of Results , Smilax , Chemistry , Tannins
9.
Article in Chinese | WPRIM | ID: wpr-308616

ABSTRACT

The rhizome of Alpinia officinarum is a widely used Chinese herbal medicine. The essential oil in A. officinarum rhizome is mainly composed of 1, 8-cineole and other monoterpenes, as the major bioactive ingredients. In plants, monoterpenes are synthesized through the methylerythritol phosphate (MEP) pathway in the plastids, and 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) is an enzyme catalyzing a committed step of the MEP pathway. In the present study, the full-length cDNA encoding DXR was cloned from the rhizome of A. officinarum, using homology-based RT-PCR and rapid amplification of cDNA ends (RACE) techniques. The new cDNA was designated as AoDXR and submitted to GenBank to be assigned with an accession number HQ874658. The full-length cDNA of AoDXR was 1 670 bp containing a 1 419 bp open reading frame encoding a polypeptide of 472 amino acids with a calculated molecular mass of 51.48 kDa and an isoelectric point of 6.15. Bioinformatic analyses revealed that AoDXR showed extensive homology with DXRs from other plant species and contained a conserved plastids transit peptide, a Pro-rich region and two highly conserved NADPH-binding motifs in its N-terminal region characterized by all plant DXRs. The phylogenetic analysis revealed that AoDXR belonged to angiosperm DXRs. The structural modeling of AoDXR showed that AoDXR had the typical V-shaped structure of DXR proteins. The tissue expression pattern analysis indicated that AoDXR expressed strongly in leaves, weak in rhizomes of A. officinarum. Exogenous methyl jasmonate (MeJA) could enhance the expression of AoDXR and the production of 1, 8-cineole in A. officinarum rhizomes. The cloning and characterization of AoDXR will be helpful to reveal the molecular regulation mechanism of monoterpene biosynthesis in A. officinarum and provides a candidate gene for metabolic engineering in improving the medicinal quality of A. officinarum rhizome.


Subject(s)
Aldose-Ketose Isomerases , Genetics , Alpinia , Chemistry , Genetics , Amino Acid Sequence , DNA, Complementary , Genetics , Gene Expression Regulation, Enzymologic , Gene Expression Regulation, Plant , Molecular Sequence Data , Monoterpenes , Metabolism , Phylogeny
10.
Article in Chinese | WPRIM | ID: wpr-295401

ABSTRACT

<p><b>OBJECTIVE</b>To study the anatomy of Dracaena cochinchinensis systematically, and find out the distribution and detect the constituents of its resin, in order to provide substantial foundation for the formation mechanism of its red resin.</p><p><b>METHOD</b>The microscopic structures of D. cochinchinensis were systematically observed by using color micrographics, including stem with and without resin, roots, barks and leaves. The HPLC fingerprints of the stem with and without resin were compared.</p><p><b>RESULT</b>Characteristics of the tangentical longitudinal section of stem with resin and surface view of leaves were elucidated. Besides xylem vessels and fibers of the stem, it was found that the red resin also exists in the cortex parenchyma cells of the stem and the medulla and xylem of the root. According to the HPLC fingerprint analysis result of the stems with and without resin, a number of flavones and stilbenoids were detected in the stem in which resin appeared after it wounded.</p><p><b>CONCLUSION</b>No secretory tissue to secrete resin was found in D. cochinchinensis, further study is needed to elucidate the formation mechanism of its resin.</p>


Subject(s)
Dracaena , Chemistry , Metabolism , Plants, Medicinal , Chemistry , Metabolism , Resins, Plant , Chemistry , Metabolism
11.
Article in Chinese | WPRIM | ID: wpr-245984

ABSTRACT

<p><b>OBJECTIVE</b>To study the chemical constituents from the root of Hedysarum multijugum.</p><p><b>METHOD</b>The compounds were separated by chromatographic methods, their structures were identified by spectral analysis.</p><p><b>RESULT</b>Four compounds were isolated and identified as soyasaponin II methyl ester (1), soyasaponin II (2), soyasaponin Bg (3), and soyasaponin I (4).</p><p><b>CONCLUSION</b>The four compounds were obtained from the plant for the first time. Compound 3 was isolated from the genus Hedysarum for the first time, and the NMR data of 3 was reported for the first time.</p>


Subject(s)
Fabaceae , Chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Oleanolic Acid , Chemistry , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Saponins , Chemistry
12.
Article in Chinese | WPRIM | ID: wpr-235236

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the protective effect of the roots of F. hirta against the cocaine-induced hepatotoxicity and it's active components.</p><p><b>METHOD</b>Cocaine hydrochloride was subcutaneously injected to make male ICR mice liver wounded. Male ICR mice were randomly ig administered with the F. hirta decoction. The dose groups are 100, 200, 300 g x kg(-1) herb materials per body weight. Cocaine hydrochloride was subcutaneously injected into the mice after the administration. The serum ALT, AST activity and the activity of CAT in liver homogenate were assayed, and liver change of pathomorphism was evaluated to prove the effect of the F. hirta decoction on cocaine-induced hepatotoxicity. And the activity of psoralean which was separated from the F. hirta decoction by bioassay-guided fractionation, was proofed in the same method.</p><p><b>RESULT</b>We find that the F. hirta decoction shows a distinct effect on reducing serum transferase. The serum transferase and the content CAT in liver homogenate were dose-related reduced, and the histopathological examination found a significantly change of the liver tissues. And the psoralean, qua the mainly component, shows the same effect.</p><p><b>CONCLUSION</b>F. hirta has the protective effect against the cocaine-induced hepatotoxicity. Psoralean is the basis.</p>


Subject(s)
Alanine Transaminase , Blood , Animals , Aspartate Aminotransferases , Blood , Catalase , Metabolism , Chemical and Drug Induced Liver Injury , Cocaine , Drugs, Chinese Herbal , Pharmacology , Ficus , Chemistry , Ficusin , Pharmacology , Liver , Pathology , Liver Diseases , Blood , Male , Mice , Mice, Inbred ICR , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Random Allocation
13.
Article in Chinese | WPRIM | ID: wpr-272820

ABSTRACT

Based on the Chinese pharmacopeia 2000 ed and Japanese pharmacopeia 14st ed., the original plant differencesof Chinese raditional medicines and Japanese Kampo medicines were compared by making list. The differences and reasons were analyzed.


Subject(s)
China , Drugs, Chinese Herbal , Japan , Medicine, Chinese Traditional , Medicine, Kampo , Pharmacopoeias as Topic , Plants, Medicinal
14.
Acta Pharmaceutica Sinica ; (12): 196-198, 2002.
Article in Chinese | WPRIM | ID: wpr-312012

ABSTRACT

<p><b>AIM</b>To study the chemical constituents from Hedysarum multijugum.</p><p><b>METHODS</b>The compounds were separated by chromatography methods, their structures were identified by spectral analysis.</p><p><b>RESULTS</b>Six compounds were isolated and identified as beta-sitosterol (1), 7-hydroxy-4'-methoxy isoflavone (2), betulic acid (3), 1,7-dihydroxy-3, 9-dimethoxy pterocarpene (4), 5, 7-dihydroxy-8-C-prenyl-4'-methoxy isoflavone (5) and 5, 7-dihydroxy-4'-methoxy isoflavone (6).</p><p><b>CONCLUSION</b>Compound 4 is a new compound and the others were obtained from the plant for the first time.</p>


Subject(s)
Benzofurans , Chemistry , Benzopyrans , Chemistry , Fabaceae , Chemistry , Molecular Structure , Plants, Medicinal , Chemistry , Sitosterols , Chemistry , Triterpenes , Chemistry
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