Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 222
Filter
1.
Article in English | WPRIM | ID: wpr-918235

ABSTRACT

Objective@#To improve the N biomarker in the amyloid/taueurodegeneration system by radiomics and study its value for predicting cognitive progression in individuals with mild cognitive impairment (MCI). @*Materials and Methods@#A group of 147 healthy controls (HCs) (72 male; mean age ± standard deviation, 73.7 ± 6.3 years), 197 patients with MCI (114 male; 72.2 ± 7.1 years), and 128 patients with Alzheimer’s disease (AD) (74 male; 73.7 ± 8.4 years) were included. Optimal A, T, and N biomarkers for discriminating HC and AD were selected using receiver operating characteristic (ROC) curve analysis. A radiomics model containing comprehensive information of the whole cerebral cortex and deep nuclei was established to create a new N biomarker. Cerebrospinal fluid (CSF) biomarkers were evaluated to determine the optimal A or T biomarkers. All MCI patients were followed up until AD conversion or for at least 60 months. The predictive value of A, T, and the radiomics-based N biomarker for cognitive progression of MCI to AD were analyzed using Kaplan-Meier estimates and the log-rank test. @*Results@#The radiomics-based N biomarker showed an ROC curve area of 0.998 for discriminating between AD and HC. CSF Aβ42 and p-tau proteins were identified as the optimal A and T biomarkers, respectively. For MCI patients on the Alzheimer’s continuum, isolated A+ was an indicator of cognitive stability, while abnormalities of T and N, separately or simultaneously, indicated a high risk of progression. For MCI patients with suspected non-Alzheimer’s disease pathophysiology, isolated T+ indicated cognitive stability, while the appearance of the radiomics-based N+ indicated a high risk of progression to AD. @*Conclusion@#We proposed a new radiomics-based improved N biomarker that could help identify patients with MCI who are at a higher risk for cognitive progression. In addition, we clarified the value of a single A/T/N biomarker for predicting the cognitive progression of MCI.

2.
Article in Chinese | WPRIM | ID: wpr-905077

ABSTRACT

Objective:To investigate the relationship between phase behavior of curcumin (CUR) from self-nanoemulsion drug delivery system (SNEDDS) and stability of the formed nanoemulsion in artificial gastrointestinal fluid. Method:The growth rate of precipitation after dispersion of CUR-SNEDDS was expressed by the change tendency of CUR supersaturation-time curve. The effect of drug loading on crystal nucleation and growth was investigated by ultraviolet-visible spectrometry and polarized light microscope, respectively. X-ray powder diffraction (XRD) and differential scanning calorimetry (DSC) were used to analyze the precipitation forms of CUR-SNEDDS with different drug loading in artificial gastrointestinal fluid. At the same time, the effect of drug loading on the quality stability of nanoemulsion formed by CUR-SNEDDS in artificial gastrointestinal fluid was investigated. Result:In the artificial gastrointestinal fluid, with the increase of drug loading, the area under the supersaturation-time curve of CUR was increased (100% drug loading≈90% drug loading>75% drug loading), the crystallization nucleation and growth rate were accelerated (100% drug loading>90% drug loading>75% drug loading), the amorphous proportion in the precipitation composition decreased, the nanoemulsion droplets adhered and distributed unevenly, the particle size and dispersivity were increased. Conclusion:High drug loading promotes the nucleation and growth of crystals, and increases the proportion of crystal forms in the precipitation composition, which leads to the decrease in the stability of the formed nanoemulsion. Therefore, it is suggested that the drug loading of CUR-SNEDDS needs to be controlled below 90%.

3.
Journal of Clinical Hepatology ; (12): 969-972, 2021.
Article in Chinese | WPRIM | ID: wpr-875915

ABSTRACT

The liver plays an important role in the metabolism, degradation, and excretion of thyroid hormones in the normal physiological state; however, when liver injuries occur, there is a significance increase in hypothyroidism, and most patients have no obvious clinical symptoms or signs. The article summarizes the chronic liver diseases caused by various etiologies and the change in thyroid function in different stages of disease and points out that the reduction in the level of thyroid hormone has an important value in predicting the risk, condition, and prognosis of chronic liver diseases. Studies have shown that some patients with chronic liver diseases can improve the condition and prognosis by adjusting thyroid function. In addition, a series of genetic disorders or dysfunctions caused by abnormal thyroid metabolism may become new therapeutic targets for certain chronic liver diseases, which needs to be confirmed by further studies.

4.
Journal of Clinical Hepatology ; (12): 841-845 (in Chinese), 2021.
Article in Chinese | WPRIM | ID: wpr-875892

ABSTRACT

Objective To investigate the value of cytokines in bile combined with clinical indices in predicting the degree of liver injury after liver transplantation. MethodsA total of 16 patients undergoing liver transplantation who were hospitalized in Center of Organ Transplantation, The Affiliated Hospital of Qingdao University, from January to December 2018 were enrolled, and according to the level of alanine aminotransferase (ALT) on day 1 after surgery, the patients were divided into mild liver injury (ALT <500 U/L) group with 10 patients and severe liver injury (ALT >500 U/L) group with 6 patients. Bile samples were collected on days 1, 3, 5, and 7 after surgery, and MILLIPLEX assay was used to measure the levels of 17 cytokines. R software was used to perform principal component analysis (PCA) of bile cytokines and clinical indices and gene ontology (GO) enrichment analysis of bile cytokines. The two-independent-samples t-test was used for comparison of normally distributed continuous data between two groups; The Mann-Whitney U test was used for comparison of non-normally distributed continuous data between two groups. A Spearman correlation analysis was performed to evaluate the correlation between clinical indices and bile cytokines. ROC curve analysis was used to evaluate the predictive value of cytokines in bile and clinical indices for liver injury after liver transplantation. ResultsCompared with the mild liver injury group, the severe liver injury group had significantly higher expression levels of bile Fractalkine (Z=-2.828, P=0.003), soluble CD40 ligand (sCD40L) (Z=-2.850, P=0.008), interleukin-4 (Z=-2.398, P=0.017), CXCL10 (Z=-2.475, P=0.023), and macrophage inflammatory protein-1α (Z=-1844, P=0.043). The correlation analysis showed that on day 1 after liver transplantation, aspartate aminotransferase, ALT, and lactate dehydrogenase were positively correlated with the levels of several cytokines in bile (all P<0.05). The area under the ROC curve of Fractalkine, sCD40L and AST were 0.933 (0.812-1.000), 0.833 (0.589-1.000) and 0.917 (0.779-1.000), respectively, suggesting that AST and Fractalkine and sCD40L in bile on the first day after liver transplantation have significant predictive value for liver injury. The results of PCA showed that bile cytokines combined with clinical indices on day 1 after liver transplantation could better distinguish the patients with mild liver injury from those with severe liver injury. GO analysis showed that bile cytokines were associated with positive feedback regulation of external stimulus, cell chemotaxis, receptor ligand activity, cytokine activity, and cytokine-cytokine receptor interaction. ConclusionFractalkine and sCD40L in bile can predict the degree of liver injury after liver transplantation.

5.
Article in English | WPRIM | ID: wpr-875292

ABSTRACT

Objective@#To determine whether noncontrast computed tomography (NCCT) models based on multivariable, radiomics features, and machine learning (ML) algorithms could further improve the discrimination of early hematoma expansion (HE) in patients with spontaneous intracerebral hemorrhage (sICH). @*Materials and Methods@#We retrospectively reviewed 261 patients with sICH who underwent initial NCCT within 6 hours of ictus and follow-up CT within 24 hours after initial NCCT, between April 2011 and March 2019. The clinical characteristics, imaging signs and radiomics features extracted from the initial NCCT images were used to construct models to discriminate early HE. A clinical-radiologic model was constructed using a multivariate logistic regression (LR) analysis. Radiomics models, a radiomics-radiologic model, and a combined model were constructed in the training cohort (n = 182) and independently verified in the validation cohort (n = 79). Receiver operating characteristic analysis and the area under the curve (AUC) were used to evaluate the discriminative power. @*Results@#The AUC of the clinical-radiologic model for discriminating early HE was 0.766. The AUCs of the radiomics model for discriminating early HE built using the LR algorithm in the training and validation cohorts were 0.926 and 0.850, respectively.The AUCs of the radiomics-radiologic model in the training and validation cohorts were 0.946 and 0.867, respectively. The AUCs of the combined model in the training and validation cohorts were 0.960 and 0.867, respectively. @*Conclusion@#NCCT models based on multivariable, radiomics features and ML algorithm could improve the discrimination of early HE. The combined model was the best recommended model to identify sICH patients at risk of early HE.

6.
China Pharmacy ; (12): 284-288, 2021.
Article in Chinese | WPRIM | ID: wpr-872678

ABSTRACT

OBJECTIVE:To ex plore the improvement effects of small-molecule glucagon-like peptide- 1 receptor(GLP-1R) agonists(6,7-dichloro-2-methylsulfonyl-3-N-tert-butylaminoquinoxaline,DMB)on osteoporosis model mice. METHODS :C57BL/ 6J mice were randomly divided into sham operation group ,model group ,positive control group (17β-estradiol,10 μg/kg)and DMB group (1 mg/kg),with 10 mice in each group. The sham operation group received bilateral laparotomy without ovariectomy , and the other groups received bilateral ovariectomy to reproduce the osteoporosis model. At 4th week after operation ,sham operation group and model group were given constant volume of florence oil intragastrically ;administration groups were given relevant medicine intragastrically ,once a day ,for consecutive 8 weeks. After last medication ,Micro-CT was used to detect the femur microstructure ,bone mineral density (BMD),bone mineral content (BMC)and bone morphometric parameters [bone tissue volume fraction (BV/TV),trabecular thickness (Tb.Th),trabecular number (TB.N)and trabecular spacing (Tb.Sp)]. ELISA assay was used to detect the expression of bone formation indexes [bone specific alkaline phosphatase (BALP),osteoprotegerin(OPG)] and bone resorption indexes [tartrate resistant acid phosphatase (TRAP)and C-terminal cross-linked peptide of type Ⅰ collagen (CTX-Ⅰ)]. Three-point bending test was used to detect biomechanical parameters (maximum load ,stiffness,stress and Young ’s modulus)of femur of mice in each group. RESULTS :In sham operation group ,trabeculae were numerous ,thick,complete in morphological structure ,well-organized and reticular ;bone Δ 基金项目:国家自然科学基金资助项目(No.81402931);陕西省 重点研发计划项目(No.2017SF-261);西安医学院 2016年博士科研启 microstructure and bone mass were normal. Compared with 动基金项目(No.2016DOC27) sham operation group , the number of trabecular bone *讲师,博士。研究方向 :分子药理学 。E-mail:zhouying209@ decreased,the thickness became thinner ,twisted or broken , 163.com and the trabecular space increased ;the bone microstructure ·284· China Pharmacy 2021Vol. 32 No. 3 中国药房 2021年第32卷第3期 deteriorated and the bone m ass decreased ;BMD,BMC,BV/TV,Tb.Th,Tb.N and biomechanical parameters of femur were decreased significantly ,while Tb.Sp and serum levels of BLAP ,OPG,TRAP and CTX- Ⅰ were increased significantly (P<0.05 or P<0.01). Compared with model group ,the bone mass of positive control group and DMB group were increased ,while the number,thickness and shape of trabecular bone partially recovered ;BMD,BMC,BV/TV,Tb.Th,Tb.N and biomechanical parameters of femur were increased significantly (P<0.05 or P<0.01);Tb.Sp,serum levels of BLAP and OPG were increased significantly (P<0.01), while the levels of TRAP and CTX- Ⅰ were decreased significantly (P<0.05 or P<0.01). CONCLUSIONS:DMB can improve osteoporosis by increasing bone mass ,improving bone microstructure ,increasing the expression of bone formation related indexes and biomechanical parameters and decreasing bone resorption related indexes.

7.
Journal of Chinese Physician ; (12): 215-222, 2021.
Article in Chinese | WPRIM | ID: wpr-884035

ABSTRACT

Objective:To investigate the relationship between the expression of inhibin subunit Beta A (INHBA) and the clinicopathological data and its effect on proliferation, invasion and metastasis of gastric cancer cells and its possible mechanisms.Methods:Using Gene Expression Profiling Interactive Analysis (GEPIA) public database to verify the expression of INHBA mRNA in gastric cancer and adjacent tissues and its relationship with pathological stage and prognosis; the relationship between INHBA and clinical prognosis was analyzed using the Kaplan-Meier Plotter online database. Immunohistochemistry was used to confirm the correlation between protein expression level of INHBA in gastric cancer and adjacent tissues and clinical pathological staging. In vitro, the cell proliferation was detected by tetrazolium salt (MTT) method; the cell migration ability was detected by scratch test, and cell invasion and metastasis ability was detected by transwell chamber assay. The expression of INHBA and epithelial-mesenchymal transition (EMT) related proteins was detected by Western blot. Results:The GEPIA database and Kaplan-Meier Plotter online database analysis showed that INHBA mRNA was highly expressed in various cancer tissues and significantly higher in gastric cancer tissues than normal tissues ( P<0.05). The high expression of INHBA mRNA was associated with clinical stage and poor prognosis of gastric cancer ( P<0.05). The results of immunohistochemistry showed that the staining score of INHBA in gastric cancer tissues was significantly higher than that in adjacent tissues ( P<0.05), and its expression level was correlated with clinical tumor node metastasis (TNM) stage ( P<0.05). The results of MTT, scratch test and transwell chamber showed that INHBA overexpression could promote the proliferation, migration, invasion and metastasis of gastric cancer cells, while interference with INHBA expression could inhibit the proliferation, migration, invasion and metastasis of gastric cancer cells; Western blot results showed that the expression of CDH1 was down regulated and the expression of CDH2 was up-regulated after INHBA overexpression. The expression of CDH1 was up-regulated and the expression of CDH2 was down-regulated after INHBA overexpression was inhibited. Conclusions:INHBA is highly expressed in gastric cancer tissues compared with adjacent tissues. The expression level of INHBA is related to tumor progression and poor prognosis. INHBA can promote the proliferation, migration and invasion of gastric cancer MGC-803 cell line and its mechanism may be related to INHBA promoting cell EMT.

8.
Article in Chinese | WPRIM | ID: wpr-882408

ABSTRACT

Post-stroke cognitive impairment (PSCI) refers to the decline of cognitive function after stroke, including mild cognitive impairment and dementia. It is very important to prevent PSCI, and it is a direction worth exploring to find the predictors. This article reviews the research progress of the predictors of PSCI, including demographic characteristics, imaging characteristics of stroke onset, stroke itself characteristics, and molecular markers, hoping to help screen high-risk patients and intervene in time to delay the occurrence and development of PSCI.

9.
Article in English | WPRIM | ID: wpr-922471

ABSTRACT

Human dental pulp stem cells (hDPSCs) are easily obtained multipotent cells, however, their potential value in regenerative medicine is hindered by the phenotypic and functional changes after conventional monolayer expansion. Here, we employed single-cell RNA sequencing (scRNA-seq) to comprehensively study the transcriptional difference between the freshly isolated and monolayer cultured DPSCs. The cell cluster analysis based on our scRNA-seq data showed that monolayer culture resulted in a significant cellular composition switch compared to the freshly isolated DPSCs. However, one subpopulation, characterized as MCAM(+)JAG(+)PDGFRA(-), maintained the most transcriptional characteristics compared to their freshly isolated counterparts. Notably, immunofluorescent staining revealed that the MCAM(+)JAG(+)PDGFRA(-) hDPSCs uniquely located in the perivascular region of human dental pulp tissue. Flow-cytometry analysis confirmed that their proportion remained relatively stable (~2%) regardless of physiological senescence or dental caries. Consistent with the annotation of scRNA-seq data, MCAM(+)JAG(+)PDGFRA(-) hDPSCs showed higher proliferation capacity and enhanced in vitro multilineage differentiation potentials (osteogenic, chondrogenic and adipogenic) compared with their counterparts PDGFRA(+) subpopulation. Furthermore, the MCAM(+)JAG(+)PDGFRA(-) hDPSCs showed enhanced bone tissue formation and adipose tissue formation after 4-week subcutaneous implantation in nude mice. Taken together, our study for the first time revealed the cellular composition switch of monolayer cultured hDPSCs compared to the freshly isolated hDPSCs. After in vitro expansion, the MCAM(+)JAG(+)PDGFRA(-) subpopulation resembled the most transcriptional characteristics of fresh hDPSCs which may be beneficial for further tissue regeneration applications.


Subject(s)
Animals , Cell Differentiation , Dental Caries , Dental Pulp , Humans , Mice , Mice, Nude , Stem Cells
10.
Article in Chinese | WPRIM | ID: wpr-921764

ABSTRACT

The clinical comprehensive evaluation of drugs is an important basis for the return of clinical value, decision-making of medical and health authorities, and allocation of medical resources. In July 2021, the National Health Commission issued the Guidelines for the Management of Clinical Comprehensive Evaluation of Drugs(trial version 2021), which required the evaluation to be implemented from the six dimensions(safety, effectiveness, economy, innovation, suitability, and accessibility), and made detailed arrangements for the clinical comprehensive evaluation of drugs. As Chinese patent medicine differs from chemical medicines in terms of effective components and action modes, the clinical comprehensive evaluation of Chinese patent medicine should highlight the characteristics and advantages of traditional Chinese medicine(TCM) on the basis of general requirements of comprehensive clinical evaluation of drugs. At present, in the clinical comprehensive evaluation of Chinese patent medicine, unified report standards have not yet been generated, resulting in the uneven quality of existing reports. To standardize the clinical comprehensive evaluation report of Chinese patent medicine and improve its quality, the editorial team, based on the relevant policy documents of clinical comprehensive evaluation of drugs, formulated the clinical comprehensive evaluation report standards for Chinese patent medicine in combination with the previous practice and expert opinions. The report standards, containing seven sections with 15 items determined, focus on data source, evaluation content, evidence synthesis, quality control, and evaluation results supported with detailed interpretations to help researchers better understand and apply the report standards for clinical comprehensive evaluation of Chinese patent medicine, improve the report quality, and provide references for the decision-making by the national medical management authorities.


Subject(s)
China , Drugs, Chinese Herbal , Information Storage and Retrieval , Medicine, Chinese Traditional , Nonprescription Drugs , Quality Control
11.
Article in Chinese | WPRIM | ID: wpr-921722

ABSTRACT

The editorial group of the clinical practice guideline for postmenopausal osteoporosis(PMOP) with traditional Chinese medicine(TCM)(hereinafter referred to as "guideline") is composed of experts specialized in TCM orthopedics, TCM gynecology, clinical epidemiology, etc. The guideline was formulated through registration, collection and selection of clinical issues/outcome indicators, evidence retrieval and screening, preparation of systematic reviews, evaluation of evidence quality, formation of recommendations, drafting, and peer review. The syndromes and treatment of PMOP are elaborated in detail. Specifically, Liuwei Dihuang Pills and Zuogui Pills are recommended for PMOP with Yin deficiency in the liver and kidney, Qing'e Pills for PMOP with kidney deficiency and blood stasis, Yougui Pills and Jingui Shenqi Pills for PMOP with Yang deficiency in the spleen and kidney, and Er'xian Decoction for PMOP with Yin and Yang deficiency in the kidney. In addition, Duhuo Jisheng Decoction can be used to relieve pain. The commonly used Chinese patent medicines include Xianling Gubao Capsules, Qianggu Capsules, Jintiange Capsules, Gushukang Capsules, Hugu Capsules, Jinwu Gutong Capsules, and Guyuling Capsules. Acupuncture and moxibustion are also effective approaches for PMOP. The rehabilitation and daily management were carried out by exercise therapies such as Baduanjin(eight-section brocade), Wuqinxi(five-animal exercises), and Taijiquan(Tai Chi), Chinese medicine diet, health education, and fall prevention. The promotion and application of this guideline will facilitate the implementation of TCM prevention and treatment of PMOP, ensure the quality of life of PMOP patients, provide effective and safe TCM treatment measures for PMOP, and reduce the risk of fracture complications.


Subject(s)
Animals , Drugs, Chinese Herbal/therapeutic use , Female , Humans , Medicine, Chinese Traditional , Osteoporosis, Postmenopausal/drug therapy , Quality of Life , Systematic Reviews as Topic , Yin Deficiency
12.
Article in Chinese | WPRIM | ID: wpr-908302

ABSTRACT

Objective:To systematically evaluate the effect of couple-centered psychological intervention on breast cancer patients.Methods:Randomized controlled trials published in PubMed, Embase, Web of Science, Scopus, CINAHL, Cochrane Library, JBI, CNKI, Wanfang, Vip and CBM database on the effect of psychological intervention on breast cancer patients with husband and wife as the center were searched by computer. The retrieval time was from the establishment of the database to September 2020. Two reviewer independently conducted literature screening, data extraction and literature quality evaluation according to the inclusion and exclusion criteria, and Meta analysis was carried out using RevMan 5.3 software.Results:A total of 879 patients were included in 11 articles. Meta analysis results showed that the couple-centered psychological intervention not only improved the quality of life, but also improved the anxiety of breast cancer patients ( SMD=1.54, 95% CI 0.22-2.86, P=0.02) and depression ( SMD=1.22, 95% CI 0.12-2.33, P=0.03). Conclusions:After psychological intervention, the anxiety and depression of breast cancer patients were improved and the quality of life was also improved. Due to the inconsistency in the mode, frequency and evaluation index of psychological intervention, more large samples and high-quality studies are needed to further verify the effect of couple-centered psychological intervention on breast cancer patients and spouses centered on husband and wife.

13.
Article in Chinese | WPRIM | ID: wpr-879784

ABSTRACT

OBJECTIVE@#To investigate the incidence rate of infectious diseases during hospitalization in late preterm infants in Beijing, China, as well as the risk factors for infectious diseases and the effect of breastfeeding on the development of infectious diseases.@*METHODS@#Related data were collected from the late preterm infants who were hospitalized in the neonatal wards of 25 hospitals in Beijing, China, from October 23, 2015 to October 30, 2017. According to the feeding pattern, they were divided into a breastfeeding group and a formula feeding group. The two groups were compared in terms of general status and incidence rate of infectious diseases. A multivariate logistic regression analysis was used to investigate the risk factors for infectious diseases.@*RESULTS@#A total of 1 576 late preterm infants were enrolled, with 153 infants in the breastfeeding group and 1 423 in the formula feeding group. Of all infants, 484 (30.71%) experienced infectious diseases. The breastfeeding group had a significantly lower incidence rate of infectious diseases than the formula feeding group (22.88% vs 31.55%, @*CONCLUSIONS@#Breastfeeding can significantly reduce the incidence of infectious diseases and is a protective factor against infectious diseases in late preterm infants. Breastfeeding should therefore be actively promoted for late preterm infants during hospitalization.


Subject(s)
Beijing/epidemiology , Breast Feeding , China/epidemiology , Communicable Diseases/epidemiology , Female , Hospitalization , Hospitals , Humans , Incidence , Infant , Infant, Newborn , Infant, Premature , Male , Pregnancy
14.
Chinese Acupuncture & Moxibustion ; (12): 1173-1177, 2020.
Article in Chinese | WPRIM | ID: wpr-877582

ABSTRACT

OBJECTIVE@#To observe the effect of early acupoint electrical stimulation on the decline of lower limbs muscle strength in patients with intensive care unit-acquired weakness (ICU-AW) caused by septic shock.@*METHODS@#A total of 58 patients with ICU-AW caused by septic shock were randomly divided into an observation group (28 cases, 1 case dropped off ) and a control group (30 cases, 2 cases dropped off ). Patients in both groups received routine basic treatment. In the observation group, acupoint electric stimulation therapy was added at Huantiao (GB 30), Futu (ST 32), Zusanli (ST 36), Xuanzhong (GB 39) and Taichong (LR 3). Unilateral point with electrodes were applied, the SDZ-Ⅱ electronic instrument (discontinuous wave, frequency in 2 Hz, strength in 5 mA) was connected and changed to the other side after 30 min of unilateral treatment.The treatment was given 2 times daily, continued for 7 d or until the medical research council (MRC) score being 54 points or more. The changes of lower limb muscle strength MRC score, modified Rankin scale (MRS) score, bilateral quadriceps thickness and gastrocnemius pinnate angle of both groups were observed before treatment and on discharge. The time of admission to ICU, time of hospitalization, mortality during hospitalization, and mortality 28 d after discharge were compared between the two groups. The MRS scores of the two groups were followed up 28 d after discharge.@*RESULTS@#The MRC scores of lower limb muscle strength in the two groups on discharge were higher than those before treatment (@*CONCLUSION@#Early acupoint electrical stimulation can improve the lower extremity muscle decline in patients with ICU-AW caused by septic shock.


Subject(s)
Acupuncture Points , Electric Stimulation , Humans , Lower Extremity , Muscle Strength , Shock, Septic/therapy
15.
Article in Chinese | WPRIM | ID: wpr-872414

ABSTRACT

Objective: To observe the improving effect of muscle regions of meridians needling method on the upper limb function in children with cerebral palsy of spastic hemiplegic type. Methods: A total of 100 children with cerebral palsy of spastic hemiplegia type were divided into a treatment group and a control group according to the visiting sequence, with 50 cases in each group. The control group was treated with conventional rehabilitation plus conventional acupuncture treatment. The treatment group was treated with conventional rehabilitation plus muscle regions of meridians needling method. The electromyography (EMG) signal values of triceps brachii and pronator teres were detected before treatment, and 3 months and 6 months after treatment. The clinical efficacy was evaluated by Peabody developmental motor scale-fine motor (PDMS-FM) and fine motor function measure (FMFM). Results: Three and six months after treatment, the EMG signal values of triceps brachii and pronator teres, grasping scores and visual-motor integrated scores of PDMS-FM and the FMFM scores in both groups increased to varying degrees compared with the same group before treatment, and the intra-group differences were all statistically significant (all P<0.05). Six months after treatment, the results of the above three items in the treatment group were all better than those in the control group, and the differences between the groups were statistically significant (all P<0.05). Conclusion: Muscle regions of meridians needling method added on the basis of conventional rehabilitation can effectively reduce the muscle tone of upper limb and enhance the muscle strength, and improve the upper limb function in children with cerebral palsy of spastic hemiplegia type. The efficacy is superior to that of the conventional rehabilitation plus conventional acupuncture treatment.

16.
Article in Chinese | WPRIM | ID: wpr-871883

ABSTRACT

Objective:To explore the relationship between growth arrest-specific protein 6 (Gas6) and acute myocardial infarction (AMI).Methods:Patients were included from Renmin Hospital of Wuhan University between January to June 2018. A total of 103 patients with angina pectoris aged 60.20±9.35 were included as angina pectoris group. A total of 102 patients with myocardial infarction aged 58.85±9.80 were included as AMI group. A total of 130 healthy individuals aged 63.14±10.40 were included as healthy control. Spearman analysis was performed to investigate the correlations between Gas6 and risk factors of (coronary heart disease, CHD). Logistic regression was performed to investigate the risk factor of myocardial infarction. ROC (receiver operating characteristic) curve was used to analyze the diagnostic performance of Gas6 to AMI.Results:The levels of Gas6 in angina pectoris group [13.77 (10.57-17.03) ng/ ml, t=2.444, P=0.025] and AMI group[16.22 (12.70-20.09) ng/ml, t=4.965, P<0.001] was higher than control group [10.92 (8.90-14.92) ng/ml]. The levels of Gas6 in angina pectoris group was lower than AMI group ( t=3.854, P<0.001). In the sensitivity analysis excluding hypertension and diabetes, the serum Gas6 level in AMI group ( n=37) [15.05 (11.08-16.20) mg/L] was higher than that in control group [10.93 (8.91-14.93)mg/L, t=3.479, P=0.001] and angina group ( n=42) [12.85 (9.10-16.20) mg/L, t=2.639, P=0.019]. CRP (C-reactive protein), WBC (white blood cell count), Glu (fasting glucose) and Cr (creatinine) were positively correlated with Gas6, r=0.194, 0.176, 0.180 and 0.120, P value=0.010, 0.012, 0.010 and 0.002, respectively. Logistic regression showed that Gas6 was a independent factor of myocardial infarction [ OR and 95 %CI were 1.080 (1.012-1.152), P=0.020]. AUC and 95 %CI of ROC curve was 0.648 (0.572-0.723). Conclusion:The levels of Gas6 may be positively associated with myocardial infarction risk.

17.
Article in Chinese | WPRIM | ID: wpr-863796

ABSTRACT

Objective:The predictive value of LCR and BIS monitoring for delayed encephalopathy in acute severe carbon monoxide poisoning.Methods:Ninety-four patients with acute severe carbon monoxide poisoning treated in the Harrison International Peace Hospital Affiliated to Hebei Medical University between January 2017 and March 2019 were selected. According to whether DEACMP occurred within 60 days after discharge, the patients were divided into the DEACMP group ( n=33) and good prognosis group ( n=61). LCR and BIS of the two groups at different time points were compared, and the correlation between LCR and BIS was analyzed. The predictive value of LCR, BIS and the combination of the two for DEACMP was analyzed by receiver operating characteristic (ROC) curve. Results:The LCR and BIS of the DEACMP group at day 1t, 3 and 5 were significantly lower than those of the good prognosis group ( P<0.05). Pearson correlation analysis showed that there was a positive correlation between BIS and LCR in the DEACMP group at day 1, 3 and 5 ( r=0.371, P<0.05). There was also a positive correlation between LCR and BIS in the good prognosis group at day 1, 3 and 5 ( r=0.373, P<0.05). ROC curve analysis showed that the areas under the curves of LCR, BIS and the combination of the two for predicting the occurrence of DEACMP in patients with ASCOP were 0.803 (95% CI: 0.707~0.897), 0.941 (95% CI: 0.886~0.997), and 0.949 (95% CI: 0.879~1.000), respectively. Conclusions:LCR combined with BIS is of high predictive value for the occurrence of DEACMP in patients with ASCOP.

18.
Article in Chinese | WPRIM | ID: wpr-863526

ABSTRACT

Radiogenomics explores the relationship between imaging features and gene expression patterns using radiomics, which is non-invasive and can present the overall information of tumors. The application of radiomics, somewhat effective in predicting gene mutations in non-small cell lung cancer (NSCLC), has recently become a research focus. Radiomic features, combined with conventional imaging, clinical and other features, can provide multi-directional information on tumors and play an increasingly important role in the prediction and precise treatment of NSCLC-driving gene phenotypes.

19.
Article in Chinese | WPRIM | ID: wpr-861674

ABSTRACT

Background: Colon capsule endoscopy (CCE) is an innovative, safe and noninvasive endoscopic technique for the diagnosis of colorectal diseases without the need of sedation. Aims: To evaluate the accuracy and safety of the second generation CCE in diagnosing colorectal polyps. Methods: In a prospective study, 32 patients who were diagnosed as having colorectal polyps (defined as at least one polyp of 6 mm or larger in diameter; or three or more polyps of any size) by colonoscopy from Jan. 2014 to Sep. 2015 at the Xijing Hospital were enrolled. Colonoscopic polypectomy was performed on the second day of CCE. Taking the finding of therapeutic colonoscopy as gold standard, the sensitivity of CCE in diagnosing colorectal polyps was evaluated. Furthermore, the colonic cleansing, the transit time and excretion rate of the capsule, as well as the CCE-related adverse events were assessed. Results: In one patient, no polyp was found under the therapeutic colonoscopy. CCE detected the polyps in 27 of 31 patients, the per-patient sensitivity was 87% (95% CI: 69%-96%). The sensitivity of CCE in detecting polyps ≥10 mm, ≥6 mm and ≤5 mm in diameter were 82% (95% CI: 48%-97%), 76% (95% CI: 54%-90%), and 66% (95% CI: 53%-77%), respectively. The overall cleanliness was adequate in 71% (95% CI: 51%-87%) of the patients. The mean gastrointestinal transit time of the capsule was (11.42±5.23) hours, and the mean colonic transit time was (7.47±4.75) hours. The capsule excretion rate within the battery life was 75%. There were no adverse events related to CCE. Conclusions: The second generation CCE is safe and has a potential value in diagnosing colorectal polyps. The sensitivity in detecting polyps of 10 mm and 6 mm or larger in diameter is fairly well.

20.
Article in Chinese | WPRIM | ID: wpr-846381

ABSTRACT

Objective: To investigate the iridoids from the roots of Valerianajatamansi and their anti-influenza virus activity. Methods: The compounds were isolated and purified over several chromatographic methods, such as silica gel, Sephadex LH-20, HPLC and so on. Their structures were identified by the analysis of their physicochemical properties and NMR data. Their anti-influenza virus activity was evaluated by A/WSN/33/2009 (H1N1) infected MDCK cells. Results: Twenty known iridoids (1-20) were isolated from the EtOAc-soluble fraction of 95% EtOH extract of V. jatamansi, and their structures were identified as baldrinal (1), 11-methoxyviburtinal (2), desacylbaldrinal (3), isovaltrate (4), deacetylisovaltrate (5), 10-isovaleroxy-valtrathydrin (6), jatamanvaltrate Q (7), valeriandoid F (8), isovaltrate acetoxyhydrin (9), jatamanvaltrate K (10), rupesin B (11), patriscadoid II (12), jatamanvaltrate W (13), patriscadoid I (14), valeriandoid D (15), valjatrate E (16), jatamanin C (17), (1S,3R,5S,7S,8S,9S)-1-methoxy-7-hydroxy-8-methyl-3,8-epoxy-Δ4,11-dihyronepetane (18), (3S,4S,5S,7S,8S,9S)-3,8-ethoxy-7-dihydroxy-4,8-dimethylperhydrocyclopenta[c]pyran (19) and 8,9-didehydro-7-hydroxy-dolichodial (20). Conclusion: Compounds 5, 9, 12, 14, and 20are isolated from V. jatamansi for the first time. Furthermore, compounds 4 and 9 exhibit anti-influenza A virus activity with IC50 values of 85.45 and 19.26 μmol/L.

SELECTION OF CITATIONS
SEARCH DETAIL