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Article in Chinese | WPRIM | ID: wpr-879011


Based on the investigation of wild medicinal plant resources in Dexing city, Jiangxi province, and the collected plant specimens, which were identified by taxonomy, two new record species of geographical distribution were found, which are Meehania zheminensis A. Takano, Pan Li & G.-H. Xia and Corydalis huangshanensis L.Q.Huang & H.S.Peng. The voucher specimens are kept in Dexing museum of traditional Chinese medicine. In this paper, the new distribution species were reported, which provides valuable information for further enriching and supplementing the species diversity of medicinal plant resources in Jiangxi province.

China , Corydalis , Humans , Lamiaceae , Medicine, Chinese Traditional , Museums , Plants, Medicinal
Article in Chinese | WPRIM | ID: wpr-687349


The plants of Chloranthaceae are widely distributed and rich in resources in our country, records of ancient herbs indicaded that various species of plants of Chloranthaceae can be used for medicinal purposes, especially the Sarcandra glabra which with the least toxicity and possessed the function of clearing heat and cooling blood, activating blood to eliminate spots and removing wind and dredging collaterals. Based on the theory of herbage and by the method of consulting the past herbal literature, we summarized and analyzed the medicinal history, distribution characteristics of herbage geography, characteristics of herbage ecology, standard collection situation and the modern toxicology research of Chloranthaceae plants. Therefore we explained the scientificity for selection of medicinal herbs of Chloranthaceae plants, and provided a theoretical and scientific basis for further research of Chloranthaceae plants.

Article in Chinese | WPRIM | ID: wpr-852984


Objective: To study and establish the UPLC of Salvia miltiorrhiza stems and leaves (SMSL) and analyze the specific peaks composition by ESI-QTOF/MS, and provide scientific basis for the comprehensive development and utilization of resources SMSL. Methods: The fingerprint of SMSL was established by UPLC, the samples were conducted by Acquity UPLC BEH C18 (100 mm×2.1 mm, 1.7 μm) and eluted with acetonitrile and 0.1% formic acid at the flow rate of 0.4 mL/min. The detection wavelength was set at 280 nm and column temperature was 35℃. Negative ion mode was chosen for qualitative analysis. The capillary voltage was set at 3.0 kV. The nebulization gas was set to 800 L/h at 400℃, and the source temperature was 120℃. The similarity evaluation, cluster analysis (CA), and principal component analysis (PCA) were used to deal with the experimental data, in order to find out the similarities and differences among the 12 batches of SMSL from five different areas. Results: The specific chromatogram of SMSL was obtained, and 13 common peaks were identified by ESI-QTOF/MS. Similarities of the 12 batches of samples were 0.823-0.997, the results of CA and PCA were consistent with similarity evaluation. Conclusion: The establishment of UPLC fingerprint of SMSL and the application of chemical pattern recognition can provide a more comprehensive reference for the quality control of SMSL and the utilization value of non-medicinal parts of S. miltiorrhiza.

Article in Chinese | WPRIM | ID: wpr-852386


Objective To evaluate the anti-oxidative effect and chemical constituents of stems and leaves of Salvia miltiorrhiza (SMSL) collected in July and December, water extracts and alcohol extracts of SMSL collected in July and December were taken as the subject, therefore provide scientific basis for the comprehensive development and utilization of SMSL. Methods The chemical constituents in the extracts were identified and determined by Ultra-high performance liquid chromatography combined with triple quadrupole electrospray tandem mass spectrometry (UPLC-TQ/MS), then confirmed the main salvianolic acids (danshensu, caffeic acid, rosmarinic acid, and salvianolic acid B) as the material basis of anti-oxidant activity of SMSL. Moreover, based on the anti-oxidant activity evaluation index: 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzthiazoline- 6-sulphonate) (ABTS) free radical scavenging and iron reduction/anti-oxidant capacity (FRAP), anti-oxidant activity of SMSL was evaluated. Meanwhile Salvia Miltiorrhiza Radix et Rhizoma (SM) from market was used to be control. Results It showed that the water extracts of SMSL in July possessed strong anti-oxidant activities, and the total salvianolic acids with the content of 75.663 mg/g was the highest; Followed by the alcohol extracts of SMSL in July, anti-oxidant activity and total phenolic acid contents of SM extracts were all lower than that of SMSL in July. Danshensu, caffeic acid, rosmarinic acid, and salvianolic acid B showed obvious anti-oxidative activities and significant dose-dependent effect in scavenging free radicals. Conclusion It revealed that SMSL possessed strong in vitro anti-oxidant activity. Additonally, it is shown that SMSL was rich in salvianolic acids, in which danshensu, caffeic acid, rosmarinic acid, and salvianolic acid B also had obvious anti-oxidant activity.

Article in Chinese | WPRIM | ID: wpr-853291


Phenolic acids with significant biological activity and pharmacological effects are a class of active ingredients in the roots and rhizomes of Salvia miltiorrhiza used for the treatment of cardiovascular and cerebrovascular diseases. With increasing the demand of clinical and market, enhancing the contents and utilization efficiency of phenolic acids in the roots and rhizomes of S. miltiorrhiza has the important practical significance for sustainable development. In this paper, the research progress in study on genomics, proteomics, and metabonomics in recent years was carried out to summarize the biosynthesis pathway, key enzymes genes, regulation mechanism, and bioconversion and utilization of phenolic acids in the roots and rhizomes of S. miltiorrhiza, which lays the scientific foundation for the efficient production and comprehensive utilization of phenolic acids resources in S. miltiorrhiza.