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1.
Journal of Experimental Hematology ; (6): 1649-1653, 2019.
Article in Chinese | WPRIM | ID: wpr-775670

ABSTRACT

OBJECTIVE@#To identify the blood group of a patient with DEL phenotype combined with positive direct anti-human globulin test and to analyze the pedigree.@*METHODS@#Routine serological reagents were used for serological analysis of RhD blood group in the pedigree members. Exons and flanking sequences of RHD gene were amplified, sequenced and analyzed for heterozygosity. The familial genetic state of DEL phenotype was further analyze in the family members.@*RESULTS@#The DAT was strongly positive in the proband. The 1227G>A allele (RHD*DEL1) was present in the exon 9 of RHD gene, and the mother was the carrier of RHD*DEL1. The proband was identified as RHD+/RHD-, suggesting the CDe/Cde haplotype.@*CONCLUSION@#The proband is DEL phenotype (RHD*DEL1).


Subject(s)
Alleles , Exons , Genotype , Humans , Pedigree , Phenotype , Rh-Hr Blood-Group System
2.
Journal of Experimental Hematology ; (6): 1800-1803, 2018.
Article in Chinese | WPRIM | ID: wpr-774383

ABSTRACT

OBJECTIVE@#To investigate the phenotype and genotype of the weak D blood group in one case of Chinese Han people.@*METHODS@#Phenotype of blood sample was identified with serologic tests; PCR-SBT was applied for the analysis of genotype and RhD zygosity.@*RESULTS@#Both saline and gel card tests demonstrated this case to be dCcee, which was contrary to glass bead card result. Some of the RBC D epitopes were negative.c.1022T>A allele was identified with PCR-SBT and the zygosity analysis showed this case to be D/d.@*CONCLUSION@#RHD*1022 A is more suitable to be categorized as weak partial D other than weak D in a Chinese Han people.


Subject(s)
Alleles , Asians , Exons , Genotype , Humans , Phenotype , Rh-Hr Blood-Group System
3.
Pakistan Journal of Pharmaceutical Sciences. 2016; 29 (2): 497-502
in English | IMEMR | ID: emr-176382

ABSTRACT

The viscera of 10 kinds of marine fishes were collected for fish oil extraction and detection of DHA and EPA, two most important polyunsaturated fatty acids. The fish oil extraction ratio for the evaluated fishes varied from 0.95% to 10.18% [wt%]. Pseudosciaena crocea presented the highest fish oil yield, followed by Mustelus manazo, Hippoglossus and Sciaenopsocellatus. A gas chromatography method was then established for analysis of EPA/DHA. The EPA concentration [in methyl ester form] in the fish oil varied from 1.39 to 10.65[mg/g]. Epinephelus awoara presented the highest EPA concentration [p<0.05], followed by Epinephelussp, Sciaenopsocellatus and Hippoglossus. The DHA concentration [in methyl ester form] in the fish oil varied from 0.58 to 37.02 [mg/g]. Epinephelus awoara presented the highest DHA concentration [p<0.05], followed by Sciaenopsocellatus, Pseudosciaena crocea and Hippoglossus. No strict positive correlation between the EPA/DHA concentration and the sea depth where the fish live was observed. The fishes living in middle depth presented highest EPA/DHA concentration


Subject(s)
Animals , Eicosapentaenoic Acid/analysis , Docosahexaenoic Acids/analysis , Viscera , Chromatography, Gas
4.
Article in Chinese | WPRIM | ID: wpr-236337

ABSTRACT

<p><b>OBJECTIVE</b>Eplets mismatch based on HLAMatchmaker software evaluates the clinical application of kidney transplantation.</p><p><b>METHODS</b>In 239 cases of renal transplant,merits of methods of the traditional HLA six antigen matcheing criteria, cross reaction groups standard and Eplets mismatch based on HLAMatchmaker standard were compared respectively.</p><p><b>RESULTS</b>The number of mismatchs with three methods in 239 cases, were grouped according to low-high mismatchs. The results revealed that HLAMatchmaker algorithm could significantly increase the number of low mismatchs group 54 (22.6%), compared with the HIA group 19(7.9%) and CREGs group 32 (13.4%). The comparison was discovered statistical significance among the three groups (P<0.001), so the comparison between each group was.</p><p><b>CONCLUSION</b>HLAMachmaker of donor-recipients matching, is a more efficient, time-saving and high sensitivity matching solution to allograft renal transplantation.</p>


Subject(s)
Adolescent , Adult , Aged , Female , Histocompatibility Testing , Methods , Humans , Kidney Transplantation , Male , Middle Aged , Software , Transplantation, Homologous , Young Adult
5.
Chinese Medical Journal ; (24): 2218-2223, 2008.
Article in English | WPRIM | ID: wpr-350739

ABSTRACT

<p><b>BACKGROUND</b>It is still controversial as to the implementation of higher positive end-expiratory pressure (PEEP) in patients with acute respiratory distress syndrome (ARDS). This study was conducted to compare the lower and higher PEEP in patients with ARDS ventilated with low tidal volume, to investigate the relationship between the recruited lung volume by higher PEEP and relevant independent variables and to provide a bedside estimate of the percentage of potentially recruitable lung by higher PEEP.</p><p><b>METHODS</b>Twenty-four patients with ARDS were studied. A lung recruiting maneuver was performed, then each patient was ventilated with PEEP of 8 cmH(2)O for 4 hours and subsequently with PEEP of 16 cmH(2)O for 4 hours. At the end of each PEEP level period, gas exchange, hemodynamic data, lung mechanics, stress index "b" of the dynamic pressure-time curve, intrinsic PEEP and recruited volume by PEEP were measured.</p><p><b>RESULTS</b>Fourteen patients were recruiters whose alveolar recruited volumes induced by PEEP 16 cmH(2)O were (425 +/- 65) ml and 10 patients were non-recruiters. Compared with the PEEP 8 cmH(2)O period, after the application of the PEEP 16 cmH(2)O, the PaO(2)/FiO(2) ratio and static lung compliance both remained unchanged in non-recruiters, whereas they increased significantly in recruiters. Changes in PaO(2)/FiO(2) and static lung compliance after PEEP increase were independently associated with the alveolar recruitment. Analyzing the relationship between recruiting maneuver (RM)-induced change in end-expiratory lung volume and the alveolar recruitment induced by PEEP, we found a notable correlation.</p><p><b>CONCLUSIONS</b>The results of this study indicated that the potential for alveolar recruitment might vary among the ARDS population and the higher PEEP levels should be limited to recruiters. Improving in PaO(2)/FiO(2), static lung compliance after PEEP increase and the shape of the pressure-time curve could be helpful for PEEP application.</p>


Subject(s)
Acute Disease , Adult , Aged , Female , Humans , Male , Middle Aged , Positive-Pressure Respiration , Methods , Pulmonary Gas Exchange , Respiratory Distress Syndrome , Therapeutics
6.
Article in Chinese | WPRIM | ID: wpr-336409

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the value of reoperation for local recurrence of rectal carcinoma.</p><p><b>METHODS</b>The data of 62 cases with post-operative local recurrence of rectal carcinoma were analyzed retrospectively.</p><p><b>RESULTS</b>All the 62 patients received reoperation. Thirty two of those patients were treated with radical resection (16 patients combined multiple organ resection), 6 palliative resection, 11 colostomy, and 13 laparatomy only. The 1-, 3- and 5-year survival rates in the patients accepted radical resection were 90.6%, 59.4% and 18.8% respectively. But in patients undergone palliative resection and combined therapy, survival time was 6-24 months with median survival time of 16 months. The patients, accepted laparatomy and intra-abdominal chemotherapy, all died within 2-14 months postoperatively. For patients with postoperative recurrence time >5 years, <2 years and 2-5 years, the reoperation resection rates were 100%(11/11), 62.9%(22/35), and 31.3%(5/16) respectively, and there were significant differences among 3 groups (P<0.01). The rate of reoperation resection of pure local recurrence was 80.0%(32/40). The rate of reoperation resection of local recurrence, associated with near organ invasion, was 27.3%(6/22). The difference was significant(P<0.01). The reoperation resection rate of first operation with Dixon or Miles was 61.9%(26/42) and 30.0%(6/20), and the difference was significant as well(P<0.05).</p><p><b>CONCLUSIONS</b>The recurrence of rectal carcinoma still needs positive operation in order to prolong the survival time and improve the quality of life of the patient. First operative procedure, post-operative recurrence time and recurrence type are important factors of reoperative resection.</p>


Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Neoplasm Recurrence, Local , General Surgery , Postoperative Period , Rectal Neoplasms , Pathology , General Surgery , Reoperation , Retrospective Studies , Survival Rate , Young Adult
7.
Article in Chinese | WPRIM | ID: wpr-344219

ABSTRACT

An intelligent control system has been designed using the single chip and the related circuit, and with the assemble language. It is connected with the common X-ray units to control the exposure dose. The result shows that three parameters for radiography are well controlled by the intelligent control system, and auto-radiography is realized.


Subject(s)
Algorithms , Artificial Intelligence , Computer Simulation , Computer Systems , Image Processing, Computer-Assisted , Information Storage and Retrieval , Methods , Radiography , Methods , Software Design , User-Computer Interface
8.
Article in Chinese | WPRIM | ID: wpr-676352

ABSTRACT

Objective To observe the effects of losartan and amlodipine on blood pressure,plasma levels of leptin,adiponectin and norepinephrine (NE) and insulin sensitivity in obese hypertensive patients.Methods The levels of plasma leptin and adiponectin were determined by RIA and the plasma NE level was assayed by high- performance liquid chromatography.Insulin resistance index (HOMA-IR) was evaluated using the homeostasis model.Results Compared with basal levels,after 16-weeks' therapy,plasma leptin,HOMA-IR and body mass index were significantly decreased in losartan group [(35.6?18.5 vs 32.0?17.1)?g/L,P

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