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1.
Article in Chinese | WPRIM | ID: wpr-928167

ABSTRACT

Through literature research and standard retrieval, Corydalis-derived medicinal materials, the origins, and related standards were summarized. Finally, 27 medicinal materials were screened out, involving 71 species(varieties). Among them, only 11 are recorded in Chinese Pharmacopoeia(2020), National Standard for Chinese Patent Drugs·Tibetan Medicine, Tibetan Medicine Standards, and other local standards, including Corydalis Bungeanae Herba and Corydalis Herba. The names and original plants of the medicinal materials are different in different standards, and the phenomena of "same medicinal material with different names" and "same name for different medicinal materials" are prominent. Most standards only include the traits, microscopic identification, and physico-chemical property identification, with unsound quality criteria. Thus, efforts should be made to strengthen the sorting of Corydalis medicinal plants, herbal textual research, and investigation of the resources and utilization. Moreover, via modern techniques, the chemical components and medicinal material basis of different original plants should be explored and sound quality standards should be established to improve the safety and quality of Corydalis-derived medicinal materials. Summarizing Corydalis medicinal plants, Corydalis-derived medicinal materials, and related standards, this study is expected to provide a reference for the standard formulation, quality evaluation, expansion of drug sources, and rational development and utilization of Corydalis resources.


Subject(s)
Corydalis , Drugs, Chinese Herbal/chemistry , Medicine, Chinese Traditional , Medicine, Tibetan Traditional , Plants, Medicinal/chemistry , Reference Standards
2.
Article in Chinese | WPRIM | ID: wpr-906373

ABSTRACT

Codonopsis Radix, one of the bulk commodities, has been commonly used for tonification in clinical practice. Apart from the medicinal purpose, it can also be utilized as food. Among the multiple local varieties, the ones called "Luduiduoji" in Tibetan medicine cannot be neglected, which have frequently been adopted for diminishing inflammation and swelling, invigorating spleen and stomach, and tonifying Qi, etc. Considering its complex origins and frequent substitution by or confusion with other medicinal materials, this paper reviewed the Si Bu Yi Dian, Jingzhu Bencao, ministerial and local standards, modern literature on Tibetan medicine, and the results of field investigation in major Tibetan medicine hospitals and medicinal material markets of Sichuan, Qinghai and Tibet to figure out the name, original plants, medicinal parts, effects, and local varieties of Codonopsis Radix in Tibetan medicine. The results showed that the names of local varieties were diverse, many of which were transliterated into Tibetan, with "Luduiduoji" being most well-known. It was derived from 14 species in genus Codonopsis and one in Adenophora of family Campanulaceae, with Codonopsis foetens subsp. nervosa, C. thalictrifolia var. mollis, C. canescens, C. alpina, and C. pilosula being the main species. According to literature records, the roots, aerial parts, and whole plants could all be employed as medicine, but there were certain differences in their clinical applications. At present, in order to protect the medicinal resources, Tibetan medical workers mostly collect the aerial parts, which are applicable to patients with yellow water, rheumatism, Gamba disease, and leprosy. This literature review of local varieties for Codonopsis Radix and textual research on their original plants are of great significance for elevating the standard, accelerating the pharmacodynamic research, expanding the sources and promoting the rational use of Codonopsis Radix.

3.
Article in Chinese | WPRIM | ID: wpr-905975

ABSTRACT

The present study systematically evaluated the efficacy and safety of Pushen capsule (PC) in the treatment of hyperlipidemia (HPL) to provide the basis for its clinical application in the future. The randomized controlled trials (RCTs) of PC in treating HPL were comprehensively retrieved from four Chinese databases and three English databases. The included RCTs were evaluated using the Cochrane risk-of-bias tool, followed by the Meta-analysis by RevMan 5.3. Twenty-four RCTs were included, with 2 634 patients involved. The patients in the experimental group were treated with PC, PC combined with conventional therapy,or PC combined with other Chinese patent medicine. The cases in the control group were treated conventionally or by other Chinese patent medicine. Meta-analysis results showed that PC alone or in combination was superior to the treatment in the control group in improving total cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol (LDL-C),and total response rate. Fourteen trials reported adverse reactions, including seven reporting specific results. The adverse reactions were dominated by epigastric discomfort, but the difference was not statistically significant. However, affected by the quality of the included trials,the evidence strength of the conclusion of this study is graded low. More high-quality RCTs reported in detail are needed for further confirmation.

4.
Article in Chinese | WPRIM | ID: wpr-905079

ABSTRACT

Objective:To study the resources and medicinal use of Tibetan medicine Codonopsis canescens,analyze its suitable habitat,and protect its wild resources,in order to provide an important reference basis for further rational development and utilization. Method:Combined investigation methods of literature textual research,visiting investigation and on-the-spot investigation were adopted. Result:In this paper,we investigated the resources and medicinal use of C. canescens in 27 cities and counties of Sichuan,Qinghai and Tibet. Compared with historical records of C. canescens,the results showed that there was no obvious change in the distribution range,and the distribution was uneven in different places. Ganzi Prefecture, Sichuan, boasts rich resources; in particular, Luhuo county has as high as 90-120 plants/100 m2, it is estimated that the reserves per unit area of root C. canescens in this area were 3 445.32 g·m-2,which totaled 1 279.18 ton(1 ton=1 000 kg). In history,the root is the main medicinal part. However, in recent years,in order to protect resources,the aboveground part is frequently used. The clinical efficacy of Tibetan medicine is mainly to tonify Qi and activate blood circulation. It can be combined with other drugs to treat rheumatism,sores,furuncle,carbuncle and other diseases. However,the plants of Codonopsis are mixed with C. canescens in each Tibetan area,and the mixed varieties are determined according to the growing varieties of the same genus in this area. Conclusion:The reserves of wild resources of C. canescens are decreasing with the increase of clinical use and market demand of Tibetan medicine. There is serious mixture of Codonopsis plants in Tibetan areas. It is suggested paying attention to resource protection,carrying out artificial planting,establishing the standard for the medicinal use of Tibetan medicine,and promote rational and sustainable utilization of resources.

5.
Journal of Experimental Hematology ; (6): 1156-1162, 2021.
Article in Chinese | WPRIM | ID: wpr-888532

ABSTRACT

OBJECTIVE@#To investigate the clinical characteristics, diagnosis and treatment of 1 case EBV negative extranodal NK/T cell lymphoma (ENKTL) patients.@*METHODS@#The clinical manifestations, diagnosis and treatment of one case ENKTL patients with EBV negative were analyzed retrospectively.@*RESULTS@#A 46-year-old woman diagnosed as positive for exosanal NK/T cell lymphoma (EBER@*CONCLUSION@#EBV negative ENKTL is rare in clinic and easy to be misdiagnosed, so it should be distinguished from peripheral T cell lymphoma. This case was treated with EBV positive ENKTL regimen, with good short-term efficacy.


Subject(s)
CDC2 Protein Kinase , Cell Proliferation , Female , Humans , Leukemia , Lymphoma, Extranodal NK-T-Cell , Middle Aged , Positron Emission Tomography Computed Tomography , Retrospective Studies
6.
Article in Chinese | WPRIM | ID: wpr-888090

ABSTRACT

This study evaluated the outcome indicators of the randomized controlled trials(RCTs) of traditional Chinese medicine(TCM) in the treatment of postmenopausal osteoporosis(PMOP) to provide a reference for the related research. Four Chinese databa-ses(CNKI, VIP, Wanfang and CBM) and three English databases(Cochrane Library, EMbase and PubMed) were searched syste-matically to screen RCTs of TCM in the treatment of PMOP according to the pre-set criteria, and the quality of the included trials was evaluated by the Cochrane risk-of-bias tool. A total of 29 627 articles were initially retrieved, and finally, 43 articles were included, including 34 in Chinese and 9 in English. As revealed by the results, the outcome indicators of 43 RCTs were mainly divided into six categories, with biochemical indicators accounting for 54.59%, bone mineral density(BMD) for 26.57%, quality of life for 6.28%, fracture for 1.94%, safety for 5.31%(including adverse reactions or events) and others for 5.31%. Biochemical indicators showed the maximum occurrence, followed by BMD. Many problems were found in the selection of outcome indicators of the TCM RCTs in the treatment of PMOP, such as the confusion of primary and secondary outcome indicators, the lack of endpoint criteria and vital clinical outcome indicators substituted by intermediate indicators, inconsistent evaluation standard of syndrome curative efficacy and neglected blinding in the measurement of subjective outcome indicators. The problems also included importance given to the efficacy indicators instead of the adverse outcome indicators, unnormalized indicator name, large quantitative range of the indicators, unconventional application of TCM efficacy criteria, seldom used confidence interval, relative effect indicator and absolute effect indicator.


Subject(s)
Bone Density , Drugs, Chinese Herbal/therapeutic use , Female , Humans , Medicine, Chinese Traditional , Osteoporosis, Postmenopausal/drug therapy , Quality of Life , Treatment Outcome
7.
Article in Chinese | WPRIM | ID: wpr-888026

ABSTRACT

This study aims to explore the efficacy of Chinese medicine injections( CMIs) for promoting blood circulation and removing blood stasis for acute cerebral infarction from the perspectives of clinical medication and mechanism of action based on two complex network analysis methods. Firstly,the current 13 kinds of CMIs for acute cerebral infarction were obtained from 2019 List of medicines for national basic medical insurance,industrial injury insurance and maternity insurance with the method of network Meta-analysis. Secondly,with the use of network pharmacology,the mechanisms of top 2 CMIs with the highest therapeutic effect for acute cerebral infarction were explored from two levels including core target and network function enrichment. The result of network Meta-analysis showed Mailuoning Injection was superior to Danhong Injection in terms of total effectiveness rate for neurological deficit score and NIHSS score. The network pharmacology results showed that Mailuoning Injection had more core targets,interaction networks,enriched biological functions and more signaling pathways than Danhong Injection for cerebral infarction. Both two CMIs can play a role in treating cerebral infarction through core targets such as TP53 and NOS3,biological processes such as fibrinolysis,nitric oxide biosynthesis,nitric oxide-mediated signal transduction,negative regulation of apoptosis in endothelial cells and apoptosis process,as well as the signaling pathways such as PI3 K-Akt signaling pathway,HIF-1 signaling pathway and cell apoptosis signaling pathways. The results of pharmacological studies explained their differences in clinical efficacy to a certain extent. A research strategy based on curative effect should be advocated in efficacy evaluation of traditional Chinese medicine,where comparative research on clinical efficacy can be conducted firstly,and then mechanism research based on outstanding effective drugs to better provide references and basis for selection of similar competitive drugs for one disease in the clinical practice.


Subject(s)
Cerebral Infarction/drug therapy , Drugs, Chinese Herbal/therapeutic use , Endothelial Cells , Female , Humans , Injections , Medicine, Chinese Traditional , Pregnancy , Signal Transduction
8.
Article in Chinese | WPRIM | ID: wpr-846642

ABSTRACT

According to the theory of traditional Chinese medicine (TCM), spleen and stomach are the most important visceral system to maintain human life and health, and healthy spleen and stomach can help to nourish the primordial qi, so as to maintain sufficient vitality of the body. Therefore, invigorating the spleen plays an important role in the prevention and recovery of human diseases. In this paper, the correlation between the theory of invigorating the spleen of TCM and the function of modern health care was discussed, and the relationship between spleen invigorating and digestive tract protection, immunomodulation, anti-oxidation, relieving physical fatigue, helping to reduce fat and lose weight, improving sleep and other modern health care functions and its mechanism were systematically summarized and analyzed. The mechanism and material basis of important spleen-invigorating Chinese medicinal materials such as Astragalus membranaceus, Poria cocos, Dioscorea opposita, Euryale ferox, Atractylodes macrocephala and Polygonatum sibiricum and classic recipe of invigorating spleen such as Guipi Decoction, Sijunzi Decoction, Buzhong Yiqi Decoction and Lizhong Decoction were briefly reviewed, in order to provide some new ideas and methods for the development and utilization of spleen-invigorating medicine and food resources in TCM and the research and development of TCM health food.

9.
Article in Chinese | WPRIM | ID: wpr-828034

ABSTRACT

The efficacy of oral Chinese patent medicine in the treatment of acute cerebral infarction was systematically evaluated by network Meta-analysis. The literature search was conducted in three English databases(Medline, EMbase and Cochrane Library) and four Chinese databases(CNKI, VIP, WanFang and SinoMed) from inception to June 2018, and the randomized controlled trials of acute cerebral infarction were screened out according to the pre-set criteria. Two reviewers independently screened out the literature by using pre-specified eligibility criteria, and assessed the quality of included studies according to the risk of bias tool of Cochrane Handbook 5.1.0. Data analysis was conducted by using Stata 13.0 and WinBUGS 1.4.3 software. Finally, 52 RCT were included, involving 11 kinds of oral Chinese patent medicines. The results of the network Meta-analysis showed that in terms of the total effective rate, the order of efficacy was as follows: Naomaitai Capsules>Xiaoshuan Changrong Capsules>Angong Niuhuang Pills>Yangxue Qingnao Granules>Compound Danshen Dripping Pills>Naoxintong Capsules>Tongxinluo Capsules>Naoxueshu Oral Liquid>Zhuyu Tongmai Capsules>Yinxingye Tablets>Compound Danshen Tablets; in terms of neurological deficit scores, the order of efficacy was: Tongxinluo Capsules>Angong Niuhuang Pills>Compound Danshen Dripping Pills>Xiaoshuan Changrong Capsules>Yangxue Qingnao Granules>Zhuyu Tongmai Capsules>Naoxintong Capsules>Naoxueshu Oral Liquid; in terms of Barthel index score, the order of efficacy was: Xiaoshuan Changrong Capsules>Naomaitai Capsules>Naoxueshu Oral Liquid>Angong Niuhuang Pills>Tongxinluo Capsules>Zhuyu Tongmai Capsules. Although different oral Chinese patent medicines can improve these outcomes, the difference in efficacy ranking was relatively large. Because of the small number and low quality of research literature, the conclusion still needs to be proved by multi-center, large-sample, and double-blind randomized trials.


Subject(s)
Brain Ischemia , Cerebral Infarction , Drugs, Chinese Herbal , Humans , Network Meta-Analysis , Nonprescription Drugs , Stroke
10.
Article in Chinese | WPRIM | ID: wpr-827960

ABSTRACT

The purpose of this study was to evaluate the selection situation of outcome indexes in randomized controlled trials of traditional Chinese medicine(TCM) in the treatment of acute ischemic stroke and to provide suggestions for future research. In this study, four Chinese databases and three English databases were searched from January 2017 to June 2019. The randomized controlled trials of traditional Chinese medicine in the treatment of ischemic stroke were selected according to the pre-established selection criteria. Cochrane bias risk assessment tool was used to evaluate the quality of the included studies. A total of 20 651 related articles were searched, and 11 662 ones were left after eliminating the repetitions. Finally, 42 articles were included, including 36 articles in Chinese and 6 articles in English, 40 randomized controlled trials and 2 registration schemes of randomized controlled trials. The results showed that the outcome indexes of 42 randomized controlled trials were mainly divided into seven categories, that is clinical outcome index, blood biochemical index, mental state evaluation index, cerebral hemodynamics index, index of evaluating the degree of carotid artery stenosis, safety indicators and other indicators. The blood biochemical index was the one with highest frequency, followed by the clinical outcome index. TCM syndrome score was used as an indicator of curative outcome in 17 studies. After the analysis, it was found that there were many problems in selecting the outcome indexes for the randomized controlled trials of traditional Chinese medicine in the treatment of cerebral apoplexy. For example, the end point index and hard index were rarely selected as the main outcome indicators, and the vast majority of them were intermediate alternative indexes; recognized curative effect could not be obtained; there were too many kinds of outcome indicators and excessive heterogeneity, hindering the promotion of superior treatment measures of traditional Chinese medicine in clinical practice. Therefore, we should draw lessons from the establishment method of the international core index outcome index set, construct the core index outcome index set of traditional Chinese medicine in the treatment of ischemic stroke, and develop the outcome index set which accords with the curative effect characteristics of traditional Chinese medicine treatment mea-sures to solve the problems effectively.


Subject(s)
Brain Ischemia , Drugs, Chinese Herbal , Humans , Medicine, Chinese Traditional , Research Design , Stroke , Treatment Outcome
11.
Article in Chinese | WPRIM | ID: wpr-878841

ABSTRACT

To systemically evaluate the clinical efficacy and safety of oral preparation of Xiakucao with levothyroxine(LT4) on Hashimoto's thyroiditis(HT), so as to provide the evidence for its clinical application in the future. All the included studies were retrieved from four Chinese databases and three English databases from their inception to December 2019. ROB assessment tool of cochrane system and the evidence classification recommended by GRADE were used to evaluate the quality of evidences in all included studies. RevMan 5.3 was used for Meta-analysis of the outcomes. Software TSA 0.9(trail sequential analysis) was used to estimate the sample size for Meta-analysis. The results showed that 11 randomized controlled trials and totaling 1 215 patients were included. Preparation of Xiakucao combined with LT4 was adopted as intervention in experimental group, while patients in control group were treated with LT4 alone. Meta-analysis results showed that as compared with control group, the rate of total efficacy in experimental group was significant improved, including improvement of thyroid function and thyroid autoantibodies, shrinkage of thyroid gland and nodule, and improvement of clinical symptoms such as fatigue and cold intolerance(RR=1.15, 95%CI[1.09, 1.21]). The experimental group significantly decreased the serum level of thyroperoxidase antibody TPO-Ab(SMD=-0.91, 95%CI[-1.40,-0.41]), and reduced the size of left thyroid lobe(MD=-1.46, 95%CI[-1.82,-1.11]), right thyroid lobe(MD=-1.45, 95%CI[-1.96,-0.94]) and isthmus of thyroid gland(MD=-1.08, 95%CI[-1.20,-0.95]). After evaluation based on GRADEpro, the results showed that the evidence quality of all included studies was low or very low. The result of TSA showed that the cumulative sample size had reached the expected value. However, the pooled results may be affected by one study with high bias risk, with not so high effect intensity of evidences. From this review, we can see that in treatment of HT, intervention of preparation of Xiakucao combined with LT4 has advantages on improvement of clinical efficiency, decreasing serum level of TPO-Ab and shrinkage of thyroid gland. However, due to the quality of evidence, more rigorously designed and high-quality trials are needed in the future to verify the clinical efficacy and safety of preparation of Xiakucao in treating HT.


Subject(s)
Hashimoto Disease , Humans , Iodide Peroxidase , Prunella , Thyroxine
12.
Journal of Experimental Hematology ; (6): 1040-1045, 2019.
Article in Chinese | WPRIM | ID: wpr-771842

ABSTRACT

OBJECTIVE@#To study the long-term efficacy and safety of CD19 chimeric antigen receptor T cells (CAR-T) in the treatment of relapsed patients with B-cell acute lymphoblastic leukemia (ALL) after allogeneic hematopoietic stem cell transplantation (allo-HSCT).@*METHODS@#A total of 7 patients with B-cell ALL relapsed after allo-HSCT were treated with CD19 CAR-T cells from September 2015 to March 2018. Among them, 6 had hematological recurrence and 1 had positive of MRD. They all were treated with a single infusion of CAR-T cells. FC chemotherapy regimen was administered before transfusion. The median number of CAR-T cells transfused was 6.0 (range 4.0-8.6) )×10/kg. Long-term efficacy and toxicity were evaluated.@*RESULTS@#Bone marrow examination performed at d 30 after CAR-T infusion showed that all 7 patients achieved complete remission and MRD negative, grade I CRS for 1 case and grade II CRS for 6 cases, two of them had mild neurotoxicity, which was controlled by treatment. Two patients presented grade VI intestinal GVHD after CAR-T infusion. The median follow-up time was 18 months (range 12-42). Follow-up showed that two patients relapsed at 9 months and 14 months after treatment, out of 2 patients one died of progressive disease and the other reachived the hematological remission, but MRD was positive after CD22 CAR-T cell therapy. At present, five patients are disease-free survival, moreover showed complete donor chimerism. One year after CAR-T cell therapy, the results of immune reconstitution showed that CD4 level was more than 300×10/L in 5 patients who disease-free survived. Among them, 3 patients had poor recovery of immunoglobulin and received gamma globulin replacement therapy.@*CONCLUSION@#All patients are followed up for at least one year. The preliminary efficacy and safety are satisfactory. CAR-T cell infusion is an effective method for the treatment of B-ALL recurrence after allo-HSCT.


Subject(s)
B-Lymphocytes , Hematopoietic Stem Cell Transplantation , Humans , Precursor Cell Lymphoblastic Leukemia-Lymphoma , Receptors, Chimeric Antigen , T-Lymphocytes
13.
Article in Chinese | WPRIM | ID: wpr-773210

ABSTRACT

To systematically review the efficacy and safety of Huoxiang Zhengqi Pills combined with Western medicine in the treatment of acute gastroenteritis. Four Chinese databases( CNKI,VIP,Wan Fang,Sino Med) and three English databases( Cochrane Library,Medline,EMbase) were systematically and comprehensively searched from the database establishment to April 2019 to collect the randomized controlled trials( RCTs) about the treatment of acute gastroenteritis with Huoxiang Zhengqi Pills combined with Western medicine. Two investigators independently performed literature screening,data extraction and bias risk assessment. Rev Man 5. 3 software was used for data analysis. A total of 316 articles were retrieved and finally 44 studies were included in this study,involving 4153 patients. The overall quality of the included studies was generally low. Meta-analysis results showed that in the total clinical effective rate,Huoxiang Zhengqi Pills combined with conventional treatment or norfloxacin tablets was superior to conventional treatment or norfloxacin tablets alone. In terms of the time for improving clinical symptoms,Huoxiang Zhengqi Pills combined with conventional treatment or norfloxacin tablets could better relieve fever than conventional treatment or norfloxacin tablets alone. In terms of incidence of adverse reactions,there was no statistical difference between Huoxiang Zhengqi Pills combined with conventional treatment and conventional treatment alone. Other outcome measures were affected by various factors( such as inclusion of only 1 study or excessive heterogeneity among studies) and could not be concluded. Due to the limitations of the quality and quantity of included studies,this conclusion still needs to be verified by more high quality researches.


Subject(s)
Drugs, Chinese Herbal , Therapeutic Uses , Gastroenteritis , Drug Therapy , Humans , Norfloxacin , Therapeutic Uses , Randomized Controlled Trials as Topic , Treatment Outcome
14.
Article in Chinese | WPRIM | ID: wpr-789457

ABSTRACT

The HPV vaccination intention in Chinese female college students and the application of health behavior related theory are reviewed in this article. Based on the health ecological model, influencing factors of college students' HPV vaccination intention were analyzed, proposing application prospect of the information-motivation-behavioral skills model in HPV vaccine behavioral science. Thus is to be explored the behavioral intervention model of Chinese female college students' HPV vaccination.

15.
Article in Chinese | WPRIM | ID: wpr-694084

ABSTRACT

Objective This study is designed to investigate the effect of the peptide from Eucheuma on platelet aggregation and mouse tail thrombosis induced by carrageenan.Methods The peptide was purified from Eucheuma using sephdexG-50 gel chromatography and HPLC.The inhibitory effects of peptide on platelet aggregation induced by adenosine diphosphate (ADP)/thrombin were detected by light transmittance aggregometry.The platelet were treated with normal saline or Eucheuma peptides (2.5,5.0,10.0μg/ml).The platelet aggregation was detected by FLASH continuous spectral fluorescence microplate reader,and the maximum platelet aggregation rate (MAR) was calculated.The peptide (15,30,60mg/kg) and 100mg/kg clopidogrel were intraperitoneal injected into mice for 7 days to confirm the anti-thrombosis activity of the peptide in carrageenan-induced mouse tail thrombosis model.Results The platelet aggregation can be inhibited by the peptide.Comparing the maximum platelet aggregation rate induced by ADP,the results showed that control group was 100%,2.5μg/ml peptide group was 61.84%,5.0μg/ml peptide group was 71.04% and 10.0μg/ml peptide was 60.95%.The maximum platelet aggregation rate induced by thrombin was also tested.The results showed that control group was 100%,2.5μg/ml peptide group was 75.34%,5.0μg/ml peptide group was 69.96% and 10.0μg/ml peptide group was 73.74%.The thrombosis rate (2.5μg/ml peptide group was 36.89%,5.0μg/ml peptide group was 17.25%,10.0μg/ml peptide group was 22.99%,and 100mg/kg clopidogrel group was 18.96%,respectively) and the length of thrombus in mice can be reduced by the peptide (15-60mg/kg).Conclusions The carrageenan induced tail thrombosis in mice can be inhibited by the peptide of Eucheuma.It is suggested that the peptide of Eucheuma is a potential compound in anti-thrombosis.

16.
Article in Chinese | WPRIM | ID: wpr-698287

ABSTRACT

BACKGROUND:Studies have shown that tumor stem ceils are the basis and the main cause of tumor recurrence and chemotherapy failure.Therefore,the research on the drug resistance of tumor stem cells has become a hotspot in the field of stem cell research.OBJECTIVE:To analyze the biological characteristics of side population (SP) cells in the U266 cell lines in relation to drug resistance.METHODS:Flow cytometry was used to monitor the percentage of SP cells in the U266 cell lines.Fluorescence-activated cell sorter (FACS) analysis was used to isolate SP and non-SP (NSP) cells from the U266 cell lines.Further analyses of the cell cycle,multidrug resistant protein,methyl cellulose cloning assay of SP cells and NSP cells were performed.Real-time quantitative PCR was used to determine the expression of ABCG2 and MDR1 genes.RESULTS AND CONCLUSION:There were (2.46±0.35)% SP cells in U266 cells,which were most in the G0 phase.The ratio of G0/G1 in SP cells was (81.50±5.42)%,which was significantly higher than that in NSP cells [(39.85±3.21)%;P <0.05].The positive expression rates of P-gp and ABCG2 in SP cells were signficantly higher than those in NSP cells (P <0.05).The cloning efficiency of SP cells was significantly higher than that of NSP cells (P < 0.05).The mRNA expression of ABCG2 and MDR1 was also significantly higher in SP cells than in NSP cells (P < 0.05).To conclude,a small subpopulation of the isolates of U266 cell lines belong to tumor stem cell-like SP cell subset,most of which are at the G0 phase.ABCG2 and MDR1 genes are highly expressed in SP cells,which particularly plays a important role in the multidrug resistance of multiple myeloma stem cell lines.

17.
Article in Chinese | WPRIM | ID: wpr-272469

ABSTRACT

Although the traditional chemotherapy has achieved a certain effect for patients with acute myeloid leukemia (AML), but there are still limitations in terms of improving the rate of complete remission and overcome relapse after remission. The further study found that many cytogenetic molecular and epigenetic abnormalities occurred during the progression of AML, such as abnormal expression of cell surface molecules, mutation, gene aberrant methylation and so on. The drugs targeted at these changes can improve the prognosis for patients, and provide a new way for treating patients with AML. At present, the mostly targeted drugs include monoclonal antibodies CD33-Ab, tyrosine kinase inhibitor, inhibitors of DNA methyltransferases inhibitors and so on. In this review, the progress of targeted therapy in AML treatment is summarized.


Subject(s)
Antibodies, Monoclonal , Therapeutic Uses , DNA Modification Methylases , Humans , Leukemia, Myeloid, Acute , Drug Therapy , Mutation , Prognosis , Protein Kinase Inhibitors , Therapeutic Uses , Remission Induction
18.
Article in Chinese | WPRIM | ID: wpr-243409

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects and mechanisms of diethylhexylphthalate (DEHP) on morphology and function of progenitor Leydig cells (PLC) in rats.</p><p><b>METHODS</b>Twenty pregnant SD rats were randomly divided into 4 groups ( n = 5): normal control group, DEHP low dose group , middle dose group, and high dose group, which were treated from postnatal day (PND) 1 to PND 21 of the pubs with DEHP at the doses of 0, 10, 100, 750 mg/(kg · d) in 0.5 ml of corn oil by gavage respectively. At the end of the treatment, the male pups were killed and blood samples were collected for determination of serum testosterone concentration by chemiluminescence method. The body weight, testis weight and anogenital distance (AGD) were measured. The morphology of PLC was observed by light and transmission electron microscopy. The protein expression of steroidogenic acute regulatory protein(StAR) in PLC was determined by immunohistochemistry. The mRNA expression of insulin-like growth factor-I (IGF-I) in the testis was assayed by real-time PCR.</p><p><b>RESULTS</b>Compared with normal control group, the serum testosterone and AGD of male pubs from the middle and high dose groups were declined significantly (P < 0.01), the testis weight and body weight from high dose group were decreased significantly (P < 0.01), while the testis weight increased in the low dose group (P < 0.05). Under light microscope, PLC showed hyperplasia and cluster aggregation in the low dose group and focal hyperplasia in the middle and high dose group. The spermatogenic cells in seminiferous tubules showed decrease, apoptosis and unfix in the high dose group. Under transmission electron microscope, the PLC showed decreased lipid droplets, smooth endoplasmic reticulum and mitochondriae in the treated group. The mRNA expression of IGF-I increased in the low dose group, and the protein expression of StAR decreased in the middle and high dose group.</p><p><b>CONCLUSION</b>Lactating exposure to DEHP may interfere with the synthesis of testosterone of PLC in male pubs, the decrease of StAR and the damage of PLC may be involved in it.</p>


Subject(s)
Animals , Body Weight , Diethylhexyl Phthalate , Female , Germ Cells , Insulin-Like Growth Factor I , Metabolism , Lactation , Leydig Cells , Cell Biology , Male , Organ Size , Phosphoproteins , Metabolism , Pregnancy , Rats , Rats, Sprague-Dawley , Stem Cells , Cell Biology , Testis , Testosterone , Blood
19.
Article in English | WPRIM | ID: wpr-636522

ABSTRACT

Dioscin is a natural steroid saponin derived from several plants, showing potent anti-cancer effect against a variety of tumor cell lines. In the present study, we investigated the anti-cancer activity of dioscin against human LNCaP cells, and evaluated the possible mechanism involved in its antineoplastic action. It was found that dioscin (1, 2 and 4 μmol/L) could significantly inhibit the viability of LNCaP cells in a time- and concentration-dependent manner. Flow cytometry revealed that the apoptosis rate was increased after treatment of LNCaP cells with dioscin for 24 h, indicating that apoptosis was an important mechanism by which dioscin inhibited cancer. Western blotting was employed to detect the expression of caspase-3, Bcl-2 and Bax in LNCaP cells. The expression of cleaved caspase-3 was significantly increased, and meanwhile procaspase-3 was markedly decreased. The expression of anti-apoptotic protein Bcl-2 was down-regulated, whereas the pro-apoptotic protein Bax was up-regulated. Moreover, the Bcl-2/Bax ratio was drastically decreased. These results suggested that dioscin possessed potential anti-tumor activity in human LNCaP cells through the apoptosis pathway, which might be associated with caspase-3 and Bcl-2 protein family.

20.
Journal of Experimental Hematology ; (6): 1748-1752, 2014.
Article in Chinese | WPRIM | ID: wpr-340424

ABSTRACT

Acute myeloid leukemia (non APL) is a group of highly heterogeneous hematologic malignancy.In recent years, after the standard "3+7" regimen, the complete remission rate of adult patients with AML (non-APL) can be as high as 70%-80%. However, due to the existence of minimal residual disease after remission, the recurrence of the disease still inevitable, only approximately 20% to 30% of the patients enjoy longterm disease-free survival. Currently only allogeneic hematopoietic stem cell transplantation is one of the most effective treatment ways for AML. The number of transplant patients is limited, because of various reasons, such as the physical condition of patients, donor sources or economic reason. After transplantation, patients also have the possibility of recurrence, therefore, drug treatment is still important after AML remission. At present, NCCN (National Comprehensive Cancer Network) recommended high-dose cytarabine as first-line postremission therapy for patients of good prognosis group and as second-line therapy for intermediate risk group. In recent years, researchers have explored other drugs, such as the nucleoside analogues, methyltransferase inhibitors and protease inhibitors or other drugs for the treatment of adult AML patients who is in remission.In this article, the treatment of conventional medicine for the treatment of AML after complete remission is summarized.


Subject(s)
Cytarabine , Disease-Free Survival , Hematopoietic Stem Cell Transplantation , Humans , Leukemia, Myeloid , Leukemia, Myeloid, Acute , Drug Therapy , Remission Induction , Treatment Outcome
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