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1.
Article in Chinese | WPRIM | ID: wpr-906440

ABSTRACT

Objective:To investigate the taxonomic structure and diversity of endophytic fungi from <italic>Datura metel </italic>and screen the strains with anti-dermatophyte activities, so as to provide resources for the development of new lead compounds against dermatomycosis. Method:Endophytic fungi were isolated from the roots, stems and leaves of <italic>D. metel</italic> after tissue block incubation and then identified by morphological analysis and rDNA-internal transcribed spacer(ITS) sequencing. Their anti-dermatophyte activities were detected by agar diffusion assay. Result:A total of 292 strains of endophytic fungi were isolated from <italic>D. metel</italic>, belonging to 34 genera, with<italic> Fusarium</italic> (72.97%) in roots, <italic>Fusarium </italic>(37.25%) and <italic>Plectosphaerella </italic>(28.43%) in stems, and <italic>Colletotrichum </italic>(39.66%) in leaves as the dominant species. The isolation rate (89.23%), colonization rate (84.62%), and diversity index (1.82) of endophytic fungi in leaves were significantly higher than those in roots (70.48%, 70.48% and 1.23) and stems (69.39%,68.03% and 1.64). The determination of anti-dermatophyte activities of 35 endophytic fungal fermented filtrates showed that the strains exhibiting inhibitory activities against <italic>Microsporum canis</italic>, <italic>Trichosporon mucoides</italic>, <italic>Trichophyton rubrum</italic> and <italic>Candida albicans </italic>accounted for 97.14%, 71.43%, 45.71%, and 25.71%, respectively. Among them, six strains (17.14%), namely <italic>Fusarium </italic>sp. R1, <italic>Penicillium </italic>sp. R5, <italic>Aspergillus </italic>sp. R7, <italic>Metarhizium </italic>sp.<italic> </italic>S18, <italic>Diaporthe </italic>sp. S19, and <italic>Glomerella </italic>sp.<italic> </italic>L57, all inhibited the four types of cutaneous fungal pathogens. Conclusion:The endophytic fungi in <italic>D. metel</italic> are diverse, and the proportion of endophytic fungi possessing anti-dermatophyte activities is high, allowing them to serve as potential resources for the development of new anti-dermatophyte agents.

2.
Article in Chinese | WPRIM | ID: wpr-879127

ABSTRACT

As a traditional Chinese medicine, Chinese dragon's blood has multiple effects, such as activating blood to remove blood stasis, softening and dispelling stagnation, astringent and hemostasis, clearing swelling and relieving pain, regulating menstruation and rectifying the blood, so it is called "an effective medicine of promoting blood circulation". It has been widely used clinically to treat a variety of diseases. With the further research on Chinese dragon's blood, its anti-tumor medicinal value is gradually emerging. Modern pharmacological studies have shown that Chinese dragon's blood exerts anti-tumor effects mainly by inhibiting cell proliferation, inducing apoptosis, inducing DNA damage and cell cycle arrest, inducing senescence and autophagy of tumor cells, inhibiting metastasis and angiogenesis, as well as reversing multidrug resistance. This article focuses on the research progress on anti-tumor effects of Chinese dragon's blood extract and its chemical components, with a view to provide new references for the in-depth research and reasonable utilization of Chinese dragon's blood.


Subject(s)
China , Dracaena , Female , Plant Extracts , Resins, Plant
3.
Article in Chinese | WPRIM | ID: wpr-888006

ABSTRACT

Viscum plants,the evergreen perennial parasitic shrubs or subshrubs,are mainly distributed in tropical and subtropical regions. There are about 70 Viscum species around the world,including 11 species and one variety in China. Mistletoe lectins are typeⅡ ribosome-inactivating proteins( RIPs) extracted from Viscum plants with anticancer and immunoregulatory activities. Many studies have focused on the mistletoe lectins isolated from V. album in Europe and V. album var. coloratum distributed in South Korea,respectively,and several preparations,such as Iscucin Ⓡ,were developed and clinically applied for cancer treatment. Although Viscum plants are widely distributed in China,only a few studies of mistletoe lectins have been reported. The recent progress of mistletoe lectins was reviewed from extraction,purification,quantitative/qualitative detection,molecular structure,pharmacological activities,toxicities,and clinical application,aiming at providing a reference for in-depth research and utilization of mistletoe lectins produced in China.


Subject(s)
Humans , Lectins , Plant Extracts , Plant Lectins , Plant Proteins/genetics , Toxins, Biological , Viscum
4.
Article in Chinese | WPRIM | ID: wpr-921780

ABSTRACT

Alzheimer's disease(AD) patients in China have been surging, and the resultant medical burden and care demand have a huge impact on the development of individuals, families, and the society. The active component compound of Epimedii Folium, Astragali Radix, and Puerariae Lobatae Radix(YHG) can regulate the expression of iron metabolism-related proteins to inhibit brain iron overload and relieve hypofunction of central nervous system in AD patients. Hepcidin is an important target regulating iron metabolism. This study investigated the effect of YHG on the expression of a disintegrin and metalloprotease-17(ADAM17), a key enzyme in the hydrolysis of β amyloid precursor protein(APP) in HT22 cells, by mediating hepcidin. To be specific, HT22 cells were cultured in vitro, followed by liposome-mediated siRNA transfection to silence the expression of hepcidin. Real-time PCR and Western blot were performed to examine the silencing result and the effect of YHG on hepcidin in AD cell model. HT22 cells were randomized into 7 groups: control group, Aβ25-35 induction(Aβ) group, hepcidin-siRNA(siRNA) group, Aβ25-35 + hepcidin-siRNA(Aβ + siRNA) group, Aβ25-35+YHG(Aβ+YHG) group, hepcidin-siRNA+YHG(siRNA+YHG) group, Aβ25-35+hepcidin-siRNA+YHG(Aβ+siRNA+YHG) group. The expression of ADAM17 mRNA in cells was detected by real-time PCR, and the expression of ADAM17 protein by immunofluorescence and Western blot. Immunofluorescence showed that the ADAM17 protein expression was lower in the Aβ group, siRNA group, and Aβ+siRNA group than in the control group(P<0.05) and the expression was lower in the Aβ+siRNA group(P<0.05) and higher in the Aβ+YHG group(P<0.05) than in the Aβ group. Moreover, the ADAM17 protein expression was lower in the Aβ+siRNA group(P<0.05) and higher in the siRNA+YHG group(P< 0.05) than in the siRNA group. The expression was higher in the Aβ+siRNA+YHG group than in the Aβ+siRNA group(P<0.05). The results of Western blot and real-time PCR were consistent with those of immunofluorescence. The experiment showed that YHG induced hepcidin to up-regulate the expression of ADAM17 in AD cell model and promote the activation of non-starch metabolic pathways, which might be the internal mechanism of YHG in preventing and treating AD.


Subject(s)
ADAM17 Protein , Alzheimer Disease/genetics , Amyloid beta-Peptides , Drugs, Chinese Herbal/pharmacology , Hepcidins/genetics , Humans , Pueraria
5.
Article in English | WPRIM | ID: wpr-880740

ABSTRACT

Disulfide-bond A oxidoreductase-like protein (DsbA-L) is a molecular chaperone involved in the multimerization of adiponectin. Recent studies have found that DsbA-L is related to metabolic diseases including gestational diabetes mellitus (GDM), and can be regulated by peroxisome proliferator-activated receptor γ (PPARγ) agonists; the specific mechanism, however, is uncertain. Furthermore, the relationship between DsbA-L and the novel adipokine chemerin is also unclear. This article aims to investigate the role of DsbA-L in the improvement of insulin resistance by PPARγ agonists in trophoblast cells cultured by the high-glucose simulation of GDM placenta. Immunohistochemistry and western blot were used to detect differences between GDM patients and normal pregnant women in DsbA-L expression in the adipose tissue. The western blot technique was performed to verify the relationship between PPARγ agonists and DsbA-L, and to explore changes in key molecules of the insulin signaling pathway, as well as the effect of chemerin on DsbA-L. Results showed that DsbA-L was significantly downregulated in the adipose tissue of GDM patients. Both PPARγ agonists and chemerin could upregulate the level of DsbA-L. Silencing DsbA-L affected the function of rosiglitazone to promote the phosphatidylinositol 3-kinase (PI3K)-protein kinase B (PKB)/AKT pathway. Therefore, it is plausible to speculate that DsbA-L is essential in the environment of PPARγ agonists for raising insulin sensitivity. Overall, we further clarified the mechanism by which PPARγ agonists improve insulin resistance.

6.
Article in Chinese | WPRIM | ID: wpr-851959

ABSTRACT

Objective To investigate the alterations of fecal metabolites and intestinal flora during the aging in a mouse model of senescence accelerated mouse prone 8 (SAMP8). Methods The 1H-NMR metabonomics and metagenomics were applied to investigate the aging-related metabolic markers and intestinal flora, and Pearson correlation analysis was performed between metabolites and gut flora. Results Thirty-one endogenous metabolites were identified in the faeces of SAMP8 mice, of which 13 metabolites changed significantly compared with SAMR1 mice. Differential metabolites were mainly enriched in four metabolic pathways: phenylalanine, tyrosine and tryptophan biosynthesis; valine, leucine and isoleucine biosynthesis; phenylalanine metabolism; histidine metabolism. The results showed that the diversity of intestinal flora was significantly changed and the relative abundances of 10 kinds of intestinal flora were significantly changed in 10-month-old SAMP8 mice. Correlation analysis showed that Christensenellaceae was positively correlated with phenylalanine, histidine, valine, isoleucine, and uridylic acid; Dehalobacterium was negatively correlated with tyrosine, and Planococcaceae was negatively correlated with valine. Conclusion This paper reveals the changes of fecal metabolites and gut flora in SAMP8 mice, which provides experimental evidence for the study of aging progress and anti-aging actions of drugs.

7.
Acta Pharmaceutica Sinica ; (12): 1639-1646, 2017.
Article in Chinese | WPRIM | ID: wpr-779770

ABSTRACT

Learning and memory decline is an important manifestation of aging, seriously affecting the health and life quality of the elderly. Aging-related learning and memory decline is often accompanied by decreased levels of norepinephrine, dopamine and serotonin neurotransmitters in the relevant brain regions. Monoamine neurotransmitters in different brain regions bind to receptors and regulate synaptic plasticity, which play an important role in learning and memory. This article reviews the changes of monoamine neurotransmitters in different brain regions, the mechanisms in regulation of learning and memory, and the factors causing abnormal levels of neurotransmitters in the process of aging in order to better understand the mechanisms of senile learning and memory decline to facilitate drug research.

8.
Article in Chinese | WPRIM | ID: wpr-665411

ABSTRACT

BACKGROUND: The preliminary study found that domestic porous tantalum is conducive to the early adhesion and proliferation of MG63 cells, which can be used as a scaffold material for bone tissue engineering. As an optimized product of platelet-rich plasma, platelet lysate is more suitable for bone induction in the bone repair. OBJECTIVE: To further investigate the effect of platelet lysate and domestic porous tantalum scaffold constructs on the proliferation of MG63 cells and expression of integrin β1 (ITGβ1)/Vinculin/F-actin signaling pathway based on our previous findings. METHODS: MG3 cells were cultured and inoculated onto domestic porous tantalum scaffolds with the addition of 3%, 5%, 7% and 10% platelet lysates. Then, 7% as the best volume fraction of platelet lysate was screened by cell counting kit-8 method. There were four experimental groups including blank group (normally cultured MG63 cells), platelet lysate group (MG63 cells were cultured in 7% platelet lysate), porous tantalum scaffold group (MG63 cells were cultured on the domestic porous tantalum scaffold), and combined group (MG63 cells were cultured with 7% platelet lysate and porous tantalum scaffold. Scanning electron microscope was used to observe the surface morphology of domestic porous tantalum and platelet lysate-porous tantalum scaffold-MG63 cell complex. Cell counting kit-8 method was used to detect the proliferation of MG63 cells. Real-time fluorescence quantitative PCR (qPCR), immunocytochemical staining and western blot were used to detect the expression of ITGβ1, Vinculin, F-actin in MG63 cells at mRNA and protein levels. RESULTS AND CONCLUSION: Under the scanning electron microscope, MG63 cells adhered well to the scaffold surface. Compared with the blank group, the proliferation of MG63 cells could be significantly promoted by either platelet lysate or porous tantalum scaffold (P < 0.05). Moreover, the proliferation of MG63 cells was significantly improved in the combined group compared with the other three groups (P < 0.05). Findings from qPCR, immunocytochemical staining and western blot showed the highest expression of ITGβ1, Vinculin, F-actin mRNA and protein in the combined group (P < 0.05). These results indicate that platelet lysate and the domestic porous tantalum scaffold can synergistically promote the proliferation of MG63 cells, and up-regulate the expression of ITGβ1, Vinculin and F-actin mRNA and protein. Activation of the ITGβ1/Vinculin/F-actin signaling pathway may contribute to the proliferation, adhesion and differentiation of MG63 cells.

9.
Article in Chinese | WPRIM | ID: wpr-279212

ABSTRACT

The study on the effective core formulae (CEF) not only summarized traditional chinese medicine (TCM) treatment experience, but also helped reveal the underlying knowledge in the formulation of TCM prescriptions. The aim of the present paper was to investigate the method of data mining for the discovery of core effective formulae for lung cancer. In the present study, a prescription fingerprint approach was used to characterize the staged prescription information of patients. The D index was used to screen potential beneficial herbs. Then, based on a herbal compatibility network, the maximal clique searching algorithm (BK algorithm) and survival analysis were applied to discover CEF for lung cancer, and a mining analysis was made for the 322 cases from Longhua hospital. The correlation between prescriptions and survival time was analyzed by prescription fingerprints. Forty-three potentially beneficial herbs were obtained, and two CEFs were significant for the survival time by a parametric survival model based on lognormal distribution, the results were verified by a multivariate survival model. The rules of combination of the two CEFs basically conform to TCM onco-therapeutic theory of strengthening the body resistance and the actual conditions in clinic. All results showed that the established approach was feasible for discovering the core effective formulae for lung cancer and mining survival data for complex TCM onco-therapy.


Subject(s)
Algorithms , Data Mining , Drug Prescriptions , Drugs, Chinese Herbal , Chemistry , Therapeutic Uses , Humans , Lung Neoplasms , Drug Therapy , Mortality , Survival Analysis
10.
Article in Chinese | WPRIM | ID: wpr-343736

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate in vivo immunological protective efficacy and safety of expressed recombinant rotavirus epitopes in mice.</p><p><b>METHODS</b>Using the Flock House virus capsid protein as a vector, three epitopes derived from rotavirus Vp4 amino acid 223-242 [rotavirus epitope A, (REA)], 243-262 [rotavirus epitope B, (REB)], and 234-251 [rotavirus epitope C, (REC)] were genetically engineered on the surface of the vector protein and expressed in pET-3 (E. coli BL21 [DE3]) system into multiple epitopes, REABC, which comprises REA, REB, and REC. Kunming strain mice were inoculated with the recombinant epitopes REABC, and then challenged perorally by cell culture-adapted rotavirus Wa (type G1P1A) and SA11 (type G3P2). Infection syndrome was observed, and virus antigen in stools of mice and serum neutralizing antibody activities were determined and analyzed.</p><p><b>RESULTS</b>The recombinant epitopes REABC significantly induced rotavirus specific neutralyzing antibodies against WA and SA11, reduced virus reproduction, elicitted immune memory in inoculated mice, and protected inoculated mice from challenge by WA or SA11 (P<0.001).</p><p><b>CONCLUSION</b>The recombinant epitopes have high immunological protective efficacy and mild side effects in mice. It may be used as an epitope-based vaccine candidate in human.</p>


Subject(s)
Animals , Antigens, Viral , Allergy and Immunology , Capsid , Allergy and Immunology , Metabolism , Capsid Proteins , Allergy and Immunology , Epitopes , Allergy and Immunology , Escherichia coli , Genetics , Female , Genetic Vectors , Male , Mice , Random Allocation , Recombinant Proteins , Allergy and Immunology , Rotavirus , Allergy and Immunology , Rotavirus Infections , Allergy and Immunology , Viral Vaccines , Allergy and Immunology
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