ABSTRACT
Irritable bowel syndrome(IBS)is one of the most common functional gastrointestinal disorders,of which diarrhea-predominant IBS(IBS-D)accounts for the largest proportion.The pathogenesis of IBS-D is complicated and diverse,and there is currently a lack of clinically effective drugs.The establishment of animal models is an essential tool for further studies of the disease mechanisms,evaluation of clinical efficacy,and drug development,and the preparation and evaluation standards of models are important factors affecting the quality of the research.Based on the currently accepted pathogenesis of IBS-D and the previous modeling experience of our research group,this review systematically summarizes the evaluation method used in animal models of IBS-D in terms of diarrhea observation,visceral sensitivity tests,and intestinal motility tests,to provide a reference for future studies.
ABSTRACT
Septic shock refers to sepsis combined with severe circulatory disorders and cell metabolism disorders, with high incidence, high fatality rate, and dangerous condition.It is one of the leading cause of death in children in pediatric intensive care units.Therefore, early diagnosis and effective treatment of septic shock are very important.Among them, fluid resuscitation is an important part of treatment.However, the research in recent years has a lot of controversy about subsequent fluid balance management issues.This review summarized fluid balance therapy, focusing on the large dose of fluid resuscitation on the prognosis of children with septic shock, aiming to provide a reference for the future improvement of fluid therapy for children with sepsis.
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Objective To establish a method for simultaneous determination of gentiopicroside,berberine hydrochloride and baicalin in Longze Xiongdan capsule. Methods The separation was performed on a DIKMA Platisil ODS column(250 mm × 4. 6 mm, 5μm)with gradient elution using methanol and 0.1%H3PO4 aqueous solution as mobile phase. The detection wavelength was set at 275 nm. Results Linear ranges of gentiopicroside,berberine hydrochloride and baicalin concentrations were 0.019-0.38 μg(r=0.9999),0.15-3.00μg(r=1.0000)and 0.078-1.56μg(r=0.9997),respectively. Their average recoveries fell between 98.97%and 101.30%. Conclusion The method can be used as the standard for the quality control of Longze Xiongdan capsule.
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OBJECTIVE:To provide new identification method for processed medicinal material Chinese polyphaga(Eupolyph-aga sinensis,Steleophaga plancyi) and their adulterants by establishing molecular identification method based on Cytb genes. METHODS:The total DNA of Chinese polyphaga and their adulterants was extracted using modified saturation sodium chloride method. The Cytb genes of all samples were amplified with PCR using general primers REVCB2H and REVCBJ. The phylogenetic tree of all samples was constructed with Neighbor-Joining(NJ)method using MEGA 5.1 software. The sequences of the Cytb gene of all sampled were compared by using DNAMAN sofetware. The difference between genuine product and their adulterants were analyzed,and the specific primers Esin-F and Esin-R were designed for molecular identification in different regions. RESULTS:DNA extracted from processed medicinal insects was successful to amplify Cytb gene segments. The phylogenetic tree of all sam-ples was consistent with their genetic relationship. A fragment was amplified only from genuine product but not from other adulter-ants with the designed specific primers Esin-F and Esin-R. CONCLUSIONS:DNA extraction method from processed Chinese polyphaga and their adulterants have been established. Designed specific primers are highly specific to genuine product Chinese polyphaga,and can be used for the identification of Chinese polyphaga and their adulterants.