Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add filters








Language
Year range
1.
Article in Chinese | WPRIM | ID: wpr-1038857

ABSTRACT

@#Objective To investigate the changes of cognitive domain function and risk factors of cognitive dysfunction (CI) in patients with mild acute cerebral infarction.Methods A total of 195 patients with mild acute cerebral infarction in our hospital were selected as the infarction group,and 50 healthy subjects were selected as the control group.The cognitive domains scores of different infarct sites were compared between the two groups.According to the Montreal Cognitive Assessment Scale (MoCA) score,the infarction group was divided into CI group (150 cases) and non-CI group (45 cases),and the general data of the two groups were compared.Multifactorial analysis of risk factors affecting CI in patients with mild acute cerebral infarction.The Receiver Operating Characteristic (ROC) and calibration curve were used to evaluate the discrimination and accuracy of the artificial neural network model.Results Compared with the control group,the cognitive function of infarct group decreased (P<0.05).CI rate of mild acute cerebral infarction was 76.92% (150/195).Multivariate Logistic analysis showed that female,diabetes mellitus,hypertension and HSS-SP≤55 were independent risk factors for CI in patients with mild acute cerebral infarction,while high education level was a protective factor (P<0.05).ROC curve and calibration curve show that the model has good discrimination and accuracy.Conclusion Clinical attention should be paid to menopausal women,patients HBS-SP score and other risk factors to prevent and slow down the occurrence of CI.

2.
Chinese Journal of Immunology ; (12): 782-786, 2016.
Article in Chinese | WPRIM | ID: wpr-490289

ABSTRACT

Objective:To reveal the dynamic characteristics of the intracellular complement mRNA from mouse macrophage ANA-1 treated with LPS or IL-4. Methods:The polarization models of macrophage ANA-1 were established by treating with LPS(1μg/ml) and IL-4(20 ng/ml),respectively. After treating at 3,8,12 and 24 h,the total RNA were abstracted by Trizol lysis methods . The macrophage polarization were estimated by the expression of IL-1β, CCL2 and Arg-1 mRNA detected by Real-time fluorescent quantitative PCR. The intracellular complement C1q, C3, CfB and CRIg mRNA were quantitatively analyzed. Results: The mouse macrophage ANA-1 cells treated with LPS was polarized to M1 since the levels of IL-1β and CCL2 mRNA were up-regulated significantly,in which their 2-△△Ct value were up to 297. 0±31. 0 and 19. 9±3. 3 respectively at 12 h. On the other hand,the ANA-1 cells treated with IL-4 was polarized to M2 because the level of Arg-1 mRNA was obviously higher( the 2-△△Ct value of Arg-1 mRNA was up to 27.3±9.1 at 24 h)(P<0.05).The intracellular complement C1q,C3,CfB and CRIg mRNAs all were up-regulated in different polarized macrophages. The intracellular C1q and C3 mRNA in polarized M2 were significantly higher,in which the peak value of C1q and C3 were to 94. 9±12. 9 and 11. 3±2. 4 at 12 h,respectively(P<0. 05). Reversely,the CfB mRNA in polarized M1 increased obviously,in which its 2-△△Ct was to 61. 4±6. 2 at 12 h. In addition,the CRIg mRNA in both groups was only up-regulated at 24 h,in which the 2-△△Ct value was 6. 5±1. 8 in M1 and 10. 8±3. 2 in M2(P<0. 05). Conclusion: The macrophage ANA-1 cell polarization models were successfully established by treated with LPS or IL-4. The intracellular complement C1q,C3 and CRIg mRNA in polarized M2 were transcripted more than in M1. But the intracellular CfB mRNA in polarized M1 was up-regulated significantly. These results suggested that the dynamic characteristic of complement components in different polarized macrophage would be correlated with its fun-tions.

3.
Article in Chinese | WPRIM | ID: wpr-683799

ABSTRACT

Objective] To investigate the possible mechanism underlying the killing of Culex quinquefasciatus larvae by Lagenidium giganteum from the biochemical point of view. [Methods] The activities of alkaline phosphatase(AKP), acid phosphatase(ACP) and esterase(EST) were observed by using calcium cobalt method, lead nitrate method and naphthol method. Results were photomicrographed and analysed quantitatively by image analysis using computer. [Results] The activities of AKP and ACP were decreased while the activities of EST were increased among the infected groups with different infection intensities. [Conclusions] The changes of these enzyme activities might be one of the important mechanisms of action of Lagenidium giganteum against Culex quinquefasciafus larvae.

4.
Article in Chinese | WPRIM | ID: wpr-587262

ABSTRACT

Objective To construct the suppression subtracted cDNA library of deltamethrin-resistant Aedes albopictus. Methods Total RNA was extracted from the deltamethrin-resistant (R-lab) and -sensitive (S-lab) isolates, mRNA was obtained after purification. Double stranded cDNAs were synthesized after reverse transcription. Two subtractions were performed by suppression subtractive hybridization with S-lab as tester and R-lab as driver or S-lab as driver and R-lab as tester. Enriched different expressed cDNA was cloned into pMD18-T vector to construct subtractive libraries. Results The subtracted cDNA libraries contained 580 and 477 positive clones respectively. The PCR results of 150 clones picked randomly from each library showed that the positive ratio of constructed cDNA libraries was 93%, with a length of cDNA fragments ranged from 150 bp to 750 bp. Conclusion The suppression subtracted cDNA library of deltamethrin-resistant Ae. albopictus is constructed.

SELECTION OF CITATIONS
SEARCH DETAIL