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1.
Chinese Journal of Radiology ; (12): 64-69, 2021.
Article in Chinese | WPRIM | ID: wpr-884413

ABSTRACT

Objective:To evaluate the clinical feasibility and image quality of three-dimensional breath-hold gradient and spin-echo (3D BH-GRASE) sequence in magnetic resonance cholangiopancreatography (MRCP).Methods:In this prospective study, 59 patients with clinically suspected pancreaticobiliary duct disease performed MRCP with both 3D BH-GRASE and 3D respiration-triggered turbo spin-echo (3D RT-TSE) sequences on 3.0 T scanner in the Affiliated Zhangjiagang Hospital of Soochow University from November 2017 to December 2018. The overall image quality was scored independently by 3 experienced radiologists based on the visibility of different anatomical features of the pancreaticobiliary duct. For comparing the 2 sequences, the statistical difference in scan time was assessed with a paired t test; while subjective scores, signal-to-noise ratios (SNR), contrast ratios (CR) and contrast noise ratios (CNR) were compared with Wilcoxon signed rank test. Results:The scan time of 3D BH-GRASE sequence was 16.4 s while that of 3D RT-TSE was (258.6±42.2) s. Their difference was statistically significant ( t=44.073, P<0.001), with the scan time for 3D BH-GRASE shortened by 94%. The overall quality scores of 3D BH-GRASE images were better than those of 3D RT-TSE ( Z=-6.595, P<0.001). There was no statistical difference ( P>0.05) in the scores regarding the visibility of the upper, middle and lower parts of common bile duct and the first and second branches of left and right hepatic ducts. For visualizing the bottom, body, neck and duct of gallbladder, the 3D BH-GRASE sequence received a higher score than the 3D RT-TSE sequence ( P<0.001). For displaying the proximal, middle and distal segments of main pancreatic duct, the 3D RT-TSE sequence was scored higher than the 3D BH-GRASE sequence ( P<0.05). There was no significant difference of SNR between the two sequences ( Z=0.403, P=0.687), whereas CR and CNR of 3D RT-TSE MRCP were better than those of 3D BH-GRASE MRCP ( Z=6.215, P<0.001 and Z=3.046, P=0.002, respectively). Conclusion:Under the prerequisite of ensuring image quality, a proper use of 3D BH-GRASE sequence can significantly shorten the scan time and thus greatly improve the working efficiency of MRCP examination.

2.
Article in Chinese | WPRIM | ID: wpr-871760

ABSTRACT

Objective:To observe the correlation between the changes of area foveal avascular zone (FAZ), central foveal thickness (CFT), the thickness of the macular ganglion cell-inner plexiform layer (GCIPL) and the BCVA in type 2 diabetic mellitus patients with different fundus lesions.Methods:A prospective clinical study. Eighty-three eyes of 51 patients with type 2 diabetic mellitus were collected in Shengjing Hospital of China Medical University from January 2018 to January 2019, including 31 males and 20 females. The average age was 53.4±7.8 years. According to the diabetic retinopathy international clinical staging criteria, the patients were divided into the non-diabetic retinopathy (NDR) group with 17 patients (31 eyes), and the non-proliferative diabetic retinopathy (NPDR) group with 34 patients (52 eyes). And 13 control eyes of 8 age- and sex-matched healthy physical examination subjects (control group) were selected in this study. The BCVA examination was performed using the international standard visual acuity chart, which was converted intoIogMAR visual acuity. OCT was used to measure the CFT, the mean and the minimum thickness of GCIPL. OCT angiography (OCTA) examination was applied to obtain blood flow density scan images in macular area of 3 mm × 3 mm size. The superficial FAZ area was measured by Photoshop software. The differences of the superficial FAZ area, BCVA, CFT, the mean and the minimum thickness of GCIPL in each group were statistically analyzed, and to explore the correlation between the parameters. Analysis of variance was used to compare measurement data among three groups, and chi-square test was used for the comparison of counting data among three groups. The statistical correlation was evaluated using Pearson’s correlation coefficient.Results:The average area of superficial FAZ area in the control group, NDR group and NPDR group were 0.314±0.103, 0.349±0.102, 0.416±0.148 mm 2; the mean logMAR BCVA were 0.015±0.038, 0.029±0.059, 0.129±0.133; the mean CFT were 247.46±13.35, 244.13±25.09, 263.12±24.96 μm; the mean GCIPL thickness were 89.00±4.98, 86.06±4.43, 82.61±14.32 μm; the mean minimum GCIPL thickness were 84.85±3.18, 80.68±5.39, 71.19±19.94 μm, respectively. The superficial FAZ area, logMAR BCVA, CFT and the minimum thickness of GCIPL showed significant differences ( F=4.660, 11.708, 6.891, 6.333; P=0.012, 0.000, 0.002, 0.003) among these three groups. Correlation analysis showed that the logMAR BCVA was positively correlated with the superficial FAZ area ( r=0.335, P=0.001), negatively correlated with the mean GCIPL thickness ( r=-0.348, P=0.001) and the minimum GCIPL thickness (r=-0.416, P=0.000), no significant correlated with the CFT ( r=0.171, P=0.095). The superficial FAZ area was negatively correlated with the CFT ( r=-0.262, P=0.010) and the minimum GCIPL thickness ( r=-0.213, P=0.037), no significant correlated with the mean GCIPL thickness ( r=-0.179, P=0.081). Conclusions:With the aggravation of fundus lesions in patients with type 2 diabetic mellitus, the superficial FAZ area gradually expands, the minimum GCIPL thickness gradually becomes thinner and the BCVA gradually decreases. Both the superficial FAZ area and the GCIPL thickness are correlated with BCVA. The superficial FAZ area is correlated with CFT and the minimum GCIPL thickness.

3.
Chinese Journal of Dermatology ; (12): 769-773, 2020.
Article in Chinese | WPRIM | ID: wpr-870358

ABSTRACT

Objective:To investigate epidemiological trends and characteristics of gonorrhea in China in recent years, and to provide evidence for developing prevention and control strategies.Methods:A descriptive epidemiological study was conducted to analyze personal, spatial and temporal distribution of gonorrhea cases reported in China from 2015 to 2019.Results:The reported incidence rate of gonorrhea in China increased from 7.36 per 100 000 in 2015 to 10.06 per 100 000 in 2017, and decreased to 8.45 per 100 000 in 2019, with an average annual increase rate of 3.51%. From 2015 to 2019, the reported annual incidence rate in males was about 5 times that in females. During the 5 years, the reported incidence rate rapidly increased year by year in the groups aged 15 - 19 years and 20 - 24 years, with average annual increase rates of 33.67% and 22.77% respectively, but decreased or kept stable in the remaining age groups. High reported incidence rates were observed in the groups aged 25 - 29 years and 30 - 34 years from 2015 to 2018, but in the groups aged 20 - 24 years and 15 - 19 years in 2019. Gonorrhea cases were reported in all occupation groups, the largest proportion of cases was found in farmers and migrant workers (29.81% - 33.36%), and the highest average annual increase rate of 31.09% was observed in students. The reported incidence rate was high (> 12 per 100 000) in Shanghai, Zhejiang, Fujian, Guangdong, Hainan, etc., but low (< 3 per 100 000) in Hebei, Tianjin, Tibet, etc.Conclusions:In general, the reported incidence rate of gonorrhea in China had been slightly increasing in the past 5 years, and was markedly higher in males than in females. The population with high incidence of gonorrhea tended to be younger, farmers and migrant workers were the most important affected populations, and the southeast coastal areas in China showed a relatively high reported incidence rate.

4.
Chinese Journal of Dermatology ; (12): 596-601, 2020.
Article in Chinese | WPRIM | ID: wpr-870332

ABSTRACT

Objective:To investigate epidemiological trend and characteristics of genital Chlamydia trachomatis (Ct) infection in China in recent years, and to provide evidence for making Ct infection prevention and control strategies. Methods:A descriptive epidemiological study was conducted to analyze personal, spatial and temporal distribution of genital Ct infection cases reported from 105 national sexually transmitted disease (STD) surveillance sites in China from 2015 to 2019.Results:The reported incidence rate of genital Ct infection increased from 37.18 per 100 000 in 2015 to 55.32 per 100 000 in 2019, with the average annual rate of increase being 10.44%. The reported incidence rates at national STD surveillance sites ranged from 0 to 1 309.38 per 100 000, which were relatively high in Zhejiang, Guangdong, Guangxi and Hainan provinces. Increasing trends in the reported incidence rate were observed among both females and males. From 2015 to 2019, the reported annual incidence rate in males was lower than that in females, and the male-to-female reported incidence rate ratio decreased from 0.43 (22.57 per 100 000 vs. 52.74 per 100 000) in 2015 to 0.32 (27.35 per 100 000 vs. 84.55 per 100 000) in 2019. The reported incidence rates increased yearly in the groups aged more than 15 years, and the average annual rate of increase was highest in the group aged 20 - 24 years (25.81%) , followed by the group aged 15 - 19 years (22.55%) ; the sexually active population aged 20 - 34 years showed the highest reported incidence rate among all the age groups, and the reported incidence rate was up to 184.70 per 100 000 in this age group in 2019. Genital Ct infection cases were reported among all occupation groups, and the largest proportion of cases was found in unemployed population (32.86% - 38.50%) , followed by commercial service population (25.29% - 27.84%) . General hospitals reported the largest number of genital Ct infection cases (68.30% - 74.59%) , followed by maternal and child health hospitals. Conclusions:The reported incidence rate of genital Ct infection was high with an increasing trend in China from 2015 to 2019, and the southeastern coastal provinces of China showed a relatively high repoted incidence rate. Genital Ct infection affected a wide range of people in different occupations. The reported incidence rate in females was higher than that in males, and the population with a high incidence tended to be younger.

5.
Article in Chinese | WPRIM | ID: wpr-870204

ABSTRACT

Objective:To assess the diagnostic and prognostic value of lymphocyte subtyping for invasive candidiasis infection (ICI) in critically ill patients with non-neutropenic sepsis.Methods:A prospective observational cohort study was performed at Peking Union Medical College Hospital (PUMCH), 377 patients with non-neutropenic sepsis admitted to Department of Critical Care Medicine from January 2017 to November 2019 were enrolled. There were 9.0% (34/377) patients diagnosed as ICI. Vital signs, supportive care therapy and microbiological specimens were collected. Peripheral blood lymphocyte subtypes, serum globulin, complements, inflammatory factors such as interleukin(IL)-6, IL-8, IL-10 and tumor necrosis factor were detected within 24 hours after sepsis was diagnosed. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value and prognostic significance of immunological indicators for ICI. Multiple logistic regression was used to analyze the independent risk factors for ICI. Kaplan-Meier analysis was used to analyze survival.Results:The acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) score was 17.0 (13.0, 21.0) in all 377 patients. The sequential organ failure score (SOFA) was 11.0 (8.0, 13.0), and the 28-day mortality rate was 27.6% (104/377). Peripheral blood CD8 +absolute T lymphocyte count≤177 cells/μl, CD28 +CD8 +T-cell count≤81 cells/μl and 1, 3-β-D-glucan (BDG) ≥88.20 ng/L were closely correlated with the diagnosis of ICI (AUC=0.793,95 %CI 0.749-0.833, P<0.000 1;AUC=0.892,95 %CI 0.856-0.921, P<0.000 1;AUC=0.761, 95 %CI 0.715-0.803, P<0.000 1, respectively), with sensitivity of diagnosis 94.12%, 100.00%, and 88.24%; the specificity of diagnosis 81.34%, 62.39%, 63.56% respectively. Multivariate logistic regression analysis identified CD8 +T-cell count≤139 cells/μl ( OR=7.463, 95 %CI 1.300-42.831, P=0.024) and CD28 +CD8 +T-cell counts≤52 cells/μl ( OR=57.494, 95 %CI 3.986-829.359, P=0.003) as independent risk factors for higher mortality. Kaplan-Meier survival analysis suggested that CD8 +T-cell count ≤139 cells/μl ( P=0.0159) and CD28 +CD8 +T-cell count≤52 cells/μl ( P=0.000 1) were associated with higher mortality within 28 days (68.8%, 91.7%). Conclusions:Low CD28 +CD8 +T cell count in peripheral blood is closely related to the development and clinical outcome of ICI in sepsis patients, which could be used as an effective indicator for the diagnosis and prognosis prediction of ICI.

6.
Chinese Journal of Geriatrics ; (12): 155-158, 2020.
Article in Chinese | WPRIM | ID: wpr-869343

ABSTRACT

Objective:To investigate whether the basal blood glucose level before cerebral infarction has an impact on acute stress hyperglycemia.Methods:A total of 366 patients with cerebral infarction admitted into the neurology department of our hospital from February 2008 to May 2012 were enrolled in this study. Acute stress hyperglycemia was reflected by stress-induced glucose up-regulation ratio (SIGUR), and the basal glucose before cerebral infarction was reflected by glycosylated hemoglobin A1c (HbA1c). The correlation between SIGUR and HbA1c was analyzed in the different populations. The difference in SIGUR was compared among the non-diabetic group, diabetes with poor blood glucose control group (HbA1≥6.5%) and diabetes with well blood glucose control group (HbA1c<6.5%). The relationship between high level of SIGUR (Q4) and HbA1c was performed through logistic regression analysis.Results:SIGUR was correlated with HbA1c, no matter in the non-diabetic, diabetic or total populations ( r=-0.200, 0.195 and 0.324, P=0.010, 0.011 and 0.000). The level of SIGUR was higher in diabetes with poor blood glucose control group than in the non-diabetes and diabetes with well blood glucose control group ( F=25.842, P=0.000), but there was no significant difference between the non-diabetic group and diabetes with well blood glucose control group ( P>0.05). Logistic regression analysis showed that the high level of SIGUR was correlated to HbA1c in the total populations ( OR=1.460, P=0.000). In the diabetic group, the probability of higher SIGUR level was increased along with the increased HbA1c level ( OR=1.237, P=0.021), while the probability of higher SIGUR level was decreased along with the increased HbA1c level in the non-diabetic group ( OR=0.233, P=0.010). Conclusions:Acute stress hyperglycemia is correlated to the basal blood glucose before cerebral infarction, and blood glucose increases more prominently in those patients with high basal blood glucose level, especially in the diabetic patients.

7.
Article in Chinese | WPRIM | ID: wpr-753330

ABSTRACT

Objective To investigate the relationship between the stress induced glucose up-regulation and brainstem infarction. Methods Three hundred and sixty-six patients of cerebral infarction in the department of neurology were included in this study. Stress induced glucose up-regulation was reflected by stress induced glucose up-regulation ratio (SIGUT), and the level of SIGUT was compared between groups of brainstem and non-brainstem infarction. SIGUT was categorized into 4 groups according to the quartiles, and relative variables were compared among 4 groups. The level-risk relationship between high level of SIGUT and brainstem infarction was performed through logistic regression. Results The level of SIGUT was higher in group of brainstem infarction than that in group of non-brainstem infarction: 1.060 ± 0.272 vs.1.159 ± 0.301(P=0.020). The percent of male, diabetes, brainstem infarction and level of FBG, HbA1c, NIHSS was obviously different in the 4 groups of SIGUT according to the quartiles (P<0.05). High level of SIGUT was correlated to brainstem infarction in the single-factor or multivariable logistic regression adjusted for relative variable (P < 0.05). Conclusions Stress induced glucose up-regulation is correlated to the brainstem infarction, which is prone to happen in brainstem infarction, and the magnitude is more prominent.

8.
Chinese Journal of Radiology ; (12): 333-337, 2018.
Article in Chinese | WPRIM | ID: wpr-707937

ABSTRACT

Objective To investigate the value of support vector machine based MRI-radiomics in the differential diagnosis of primary hepatic carcinomas (PHCs). Methods PHCs patients were retrospectively collected from July 2013 to February 2017 in the First Affiliated Hospital of Zhejiang University.All patients underwent unenhanced and enhanced MRI liver scan before surgery,and confirmed by pathology. A total of 294 PHCs patients (305 lesions), including 96 cases (97 lesions) of massive type cholangiocarcinoma (mCC), 107(107 lesions)of hepatocellular carcinoma (HCC), and 91 (101 lesions) of mixed hepatocellular and cholangiocellular carcinomas(HCC-CC).All patients underwent unenhanced and dynamic enhanced MRI liver scan including arterial, portal venous and equilibrium phases. Two hundred and three lesions (65 mCC, 71 HCC, 67 HCC-CC) were assigned into the training set, the remaining 102 lesions(32 mCC,36 HCC,34 HCC-CC)into the validation set,according to a ratio of 2:1.The entire lesions were delineated manually using a region of interest on equilibrium phase of enhanced MRI by using a home-made dedicated software(Analysis Kit,AK,General Electrics,US).Then the least absolute shrinkage and selection operator (LASSO) regression was used to select image features with a method of 10 fold cross-validation, and to reduce the dimensionality. The spearman method was used afterwards to condense the image features by removing redundant.A predictive model of diagnosing the different types of PHCs was established based on support vector machines(SVM),and the accuracy of applying the model in the data sets was used to evaluate the diagnostic efficacy of the model. Results A total of 280 quantitative imaging features were extracted in the training set.Thirty one imaging features were selected after LASSO regression and dimensionality reduction,and 21 features were remained after redundancy removing.The SVM showed the best generalization ability when the first 11 imaging features were used due to the Hughes effect.The 11 imaging features include 4 parameters of histogram,2 of textures,4 of gray-level co-occurrence matrix and 1 of gray-level run length matrix. A predictive model for PHCs was established after the study of the 11 imaging features and a regression analysis using SVM.The accuracy of the predictive model was 80.3% (163/203) in differentiating PHCs in the training set. The accuracy of the model was 75.5% (77/102) after applying it in the validation set. The diagnostic accuracy for HCC-CC, HCC and mCC was 85.3% (29/34), 77.8% (28/36) and 62.5% (20/32), respectively, in the validation set. For HCC-CC, 3 cases were misdiagnosed as mCC and 2 cases as HCC.For HCC,3 cases were misdiagnosed as HCC-CC and 5 cases as mCC.For mCC,9 cases were misdiagnosed as HCC-CC and 3 cases as HCC.The model showed the highest accuracy in predicting HCC-CC.Conclusion Radiomics method based on SVM may have a high accuracy in predicting different pathologic types of PHC,with the highest accuracy for HCC-CC.

9.
Article in Chinese | WPRIM | ID: wpr-733701

ABSTRACT

Diabetic retinopathy (DR) is the most common microvascular complication of diabetes, which severely affects the visual function and quality of life of patients, and can lead to irreversible blindness in later stages. In recent years, some progress has been made in DR treatment, but the effect is limited. The effective prevention and treatment of DR is still the focus of current research. This article reviews the recent advances in DR auxiliary diagnosis, clinical treatment, emerging drugs, and new treatment strategies.

10.
Article in Chinese | WPRIM | ID: wpr-611210

ABSTRACT

Objective To investigate the expression of triggering receptor expressed on myeloid cells receptor-1 (TREM-1) in plasma and bronchoalveolar lavage fluid (BALF) and its correlation with Galactomannan,IFN,IL-6 and IL-10 in Aspergillus infected mice.Methods Cyclophosphamide (CTX) was intraperitoneally injected and fumigatus spore suspension was inhaled by nose to establish the immunocompromised invasive pulmonary aspergillosis (IPA) mouse model.Healthy controls,immunocompromised only and IPA only groups were also established.Each group had 6 mice.After inoculation,mice were sacrificed.Lung tissue specimens,BALF,and plasma samples were collected.Plasma and BALF soluble TREM-1 (sTREM-1),Galactomannan,IFNγ,IL-6,and IL-10 were detected by ELISA.Results Positive Aspergillus fumigatus was found by tissue culture in the lung.Infiltration of inflammatory cells,blood congestion and interstitial lung tissue injury were observed in histological sections of both IPA and immunocompromised IPA mice.Compared to IPA group [(453.78 ± 74.18) ng/L,P < 0.001;(10.21±1.46) ng/L,P<0.001] and control group [(245.16 ±65.85) ng/L,P<0.001;(6.60 ± 3.74) ng/L,P < 0.001],the plasma and BALF sTREM-1 significantly increased in immunocompromised IPA group [(1 537.64 ± 359.52) ng/L;(20.12-± 2.72) ng/L].Compared to control group,both the BALF sTREM-1 in IPA group (P =0.041) and the plasma and BALF Galactomannan,IFNγ,IL-6,and IL-10 levels in IPA and immunocompromised IPA groups were significantly higher (P <0.01).Pearson correlation analysis showed that plasma and BALF sTREM-1 were significantly correlated with Galactomannan (r =0.83,P < 0.001;r =0.82,P < 0.001),IFNγ (r =0.79,P<0.001;r=0.61,P<0.01),IL-6 (r=0.81,P<0.001;r=0.66,P<0.01),andIL-10 (r=0.70,P =0.001;r =0.54,P =0.02).Conclusions Plasma and BALF sTREM-1 appears highly expressed in Aspergillus infected mice.sTREM-1 in mice plasma and BALF is closely correlated with Galactomannan,IFNγ,IL-6,and IL-10 levels,which suggests that sTREM-1 has great diagnostic value during invasive fungal infection.

11.
Practical Oncology Journal ; (6): 71-74, 2016.
Article in Chinese | WPRIM | ID: wpr-499331

ABSTRACT

Non-melanoma skin cancers( NMSC) are one of the most common malignancy in humans.A-mong NMSC types,basal cell carcinoma(BCC)has the highest incidence and squamous cell carcinoma(SCC)is less common although it can metastasize.Over 30 years ago,Photodynamic therapy( PDT) was applied for the stud-y of human tumor,it showed the advantage of being a non-invasive option,and capable of field treatment,provi-ding optimum cosmetic outcomes.Numerous clinical research studies have shown the efficacy of PDT for treating NMSC.The purpose of this systematic review is to summarize the previous results,current evidence and the possi-bilities for PDT in patients with NMSC.

12.
Chinese Circulation Journal ; (12): 644-648, 2016.
Article in Chinese | WPRIM | ID: wpr-497271

ABSTRACT

Objective: To explore aspirin resistance (AR) phenomenon in patients with coronary artery disease (CAD) for secondary prevention and to study the relationships between AR and COX1, COX2, TBXA2R gene polymorphisms. Methods: A total of 2881 CAD patients taken aspirin (100 mg/day) in 7 consecutive days were enrolled. Among them, 2 groups were established as AR group, n=166 and Control group, n=200 aspirin sensitive patients. Platelet aggregation function was induced by arachidonic acid (AA), COX1, COX2 and TBXA2R gene polymorphisms were examined by polymerase chain reaction-restricted fragment length polymorphisms (PCR-RFLP) method. Results: The occurrence rate of AR was 5.76% (166/2881). There were 8 tagSNPs locus in 3 genes as in COX1:(rs3842788), (rs4273915), (rs7866582); in: COX2 (rs3218625); in TBXA2R: (rs2238630), (rs2238631), (rs2238633), (rs3786989). The frequencies of wild type, heterozygous genotype and homozygous genotype were similar between 2 groups. Conclusion: The incidence rate of AR is not high in CHD patients with regular aspirin medication; single nucleotide gene polymorphisms of COX1, COX2 and TBXA2R have no obvious correlation to AR.

13.
Article in Chinese | WPRIM | ID: wpr-309145

ABSTRACT

<p><b>OBJECTIVE</b>This study aims to observe the expression of Sclerostin during movement of orthodontic teeth and determine the effect of this protein on remodeling of periodontal tissues.</p><p><b>METHODS</b>Twenty-four Wistar rats were chosen. Orthodontic forces were applied between the bilateral incisor and first molar to achieve mesial movement. Rats in each group were executed at different time points (0, 1, 3, 5, 7, 14 d). Morphology of periodontal tissue was observed by hematoxylin-eosin (HE) staining. The number of osteoclasts were observed by tartrate-resistant acid phosphatase (TRAP) staining. Sclerostin expression were observed by immunohistochemical staining.</p><p><b>RESULTS</b>HE staining revealed that the resorption of alveolar bone intensified with prolonged movement. Results of immunohistochemical and TRAP staining revealed that Sclerostin expression and number of osteoclasts were related to duration of movement of orthodontic tooth. After staining for 5 days, the number of osteoclasts and Sclerostin expression reached their peak and then began to decline. The numbers of osteoclasts and the expression level of Sclerostin were higher at the compressive side than those at the tensive side.</p><p><b>CONCLUSION</b>Sclerostin affected orthodontic tooth movement by inhibiting the Wnt signaling pathway and by indirectly or directly controlling bone morphogenetic protein.</p>


Subject(s)
Animals , Bone Morphogenetic Proteins , Metabolism , Genetic Markers , Incisor , Molar , Movement , Osteoclasts , Periodontal Ligament , Metabolism , Periodontium , Rats , Rats, Wistar , Tooth Movement Techniques
14.
Article in Chinese | WPRIM | ID: wpr-446330

ABSTRACT

Objective To examine the interrelation between the cognitive deviation and loneli-ness levels of medical students and its influencing factors. Methods Totally 220 medical students in grade two of one medical school were selected by method of cluster random sampling. The data of the research were obtained through cognitive bias questionnaire (CBQ) and emotional-social loneliness questionnaire. Interrelation between the cognitive deviation type and condition of emotional-social lone-liness of medical students were analyzed by Pearson product-moment correlation two-tailed test. Multi-ple stepwise regression analysis was conducted by taking score of emotional-social loneliness as de-pendent variable and score of CBQ as independent variable. Differences in cognitive deviation and loneliness levels between rural and urban students as well as between students from single child family and students from non single child family were analyzed by independent-sample t test. Results Neg-ative cognitive deviation of depression-distortion type was positively correlated with medical students' emotional isolation (r=0.161, P=0.021),social isolation (r=0.266,P=0.000), emotional loneliness (r=0.340, P=0.000) and social loneliness (r=0.385, P=0.000). The regression equation was: score of emotional-social loneliness=27.165+1.908 (depression-distortion)+0.836 (depression-non distortion).Students from non single child family had higher scores than students from single child family in the perspectives of depression-distortion”(P=0.017), social isolation(P=0.001), emotional loneliness(P=0.016), social loneliness(P=0.000). Rural students had higher scores in the above four perspectives than urban students(P<0.05). Conclusions Negative cognitive deviation levels of medical students is positively correlated with emotion and social isolation conditions and loneliness experiences. Regres-sion analysis shows that unhealthy cognitive disposition and thinking mode probably are one of the im-portant reasons leading to medical students' stress disorder. Students from single child family and rural area may experience social loneliness more deeply and students from single child family have obvious cognitive deviation.

15.
Article in Chinese | WPRIM | ID: wpr-344513

ABSTRACT

<p><b>OBJECTIVE</b>To introduce the hairy root of Eucommia ulmoides, and screen put the excellent roots.</p><p><b>METHOD</b>Hairy roots were obtained by infecting the different explants of E. ulmoides. Aucubin content was detected by HPLC.</p><p><b>RESULT</b>The best transformation condition was embryonic axis infected by twenty minutes bacillus water dip-dye and three-days co-culture. Liquid culture was better than solid culture, and advantageous to accumulating of biomass and medical contents of hairy root. The E1 was the excellent roots.</p><p><b>CONCLUSION</b>Hairy root of E. ulmoides will be useful for the production of active components in aucubin.</p>


Subject(s)
Culture Media , Metabolism , Eucommiaceae , Chemistry , Metabolism , Glucosides , Metabolism , Iridoid Glucosides , Iridoids , Metabolism , Plant Roots , Chemistry , Metabolism , Tissue Culture Techniques
16.
Article in Chinese | WPRIM | ID: wpr-315416

ABSTRACT

<p><b>OBJECTIVE</b>Used HPLC to set up fingerprint of medicinal-plant Scutellaria tsinyunensis collected from Jinyun mountain of Chongqing, and to determine four flavonoids in its root, stem and leaf.</p><p><b>METHOD</b>Analysis was carried out on a COSMOSIL C18-PAQ column (4.6 mm x 250 mm, 5 microm) with acetonitrile and H2O-H3PO4-THF(85:12:0.3) as the mobile phases in a gradient elution, with a flow rate of 1.0 mL x min(-1). The column temperature was 30 TC, and the detection wavelength was 275 nm. The fingerprints of ten populations were determined, and the data were analyzed by the similarity evaluation software.</p><p><b>RESULT</b>The mutual mode of HPLC fingerprint was established. There were 14 common peaks in all, each peak was well separated. The peak was steady and showed good repeatability. Compared with the mutual mode, the similarities of 10 samples were 0.987 0.994, 0.993, 0.971, 0.983, 0.994, 0.981, 0.981, 0.988, 0.972, and the average similarity was 0.984. The chromatographic profiles of 10 samples were very similar. The four flavonoids were determined successfully by this method. The results showed that the content of flavonoids in the root was the most, and the leaf was the least.</p><p><b>CONCLUSION</b>This method was steady and reliable which could be applied in identification, genetic diversity analysis, protection and utilization of S. tsinyunensis.</p>


Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Flavonoids , Plant Structures , Chemistry , Scutellaria , Chemistry
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