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1.
Chinese Journal of Traumatology ; (6): 118-121, 2022.
Article in English | WPRIM | ID: wpr-928482

ABSTRACT

Intertrochanteric fractures have become a severe public health problem in elderly patients. Proximal femoral nail anti-rotation (PFNA) is a commonly used intramedullary fixation device for unstable intertrochanteric fractures. Pelvic perforation by cephalic screw is a rare complication. We reported an 84-year-old female who fell at home and sustained an intertrochanteric fracture. The patient underwent surgery with PFNA as the intramedullary fixation device. Routine postoperative examination revealed medial migration of the helical blade that eventually caused pelvic perforation. We performed a cemented total hip arthroplasty as the savage procedure. At the latest follow-up of 12 months after total hip arthroplasty, the patient had no pain or loosening of the prosthesis in the left hip. Pelvic perforation should be considered when choosing PFNA as the intramedullary fixation device, especially in patients with severe osteoporosis wherein the helical blade can be easily inserted during the operation. The lack of devices to avoid oversliding of the helical blade in PFNA is an unreported cause of this complication and should be considered in such cases.


Subject(s)
Aged , Aged, 80 and over , Bone Nails/adverse effects , Female , Femur , Fracture Fixation, Intramedullary/adverse effects , Hip Fractures/surgery , Humans , Retrospective Studies , Treatment Outcome
2.
Acta Pharmaceutica Sinica ; (12): 1465-1470, 2022.
Article in Chinese | WPRIM | ID: wpr-924751

ABSTRACT

Probe electrospray ionization (PESI) is one of the typical types of ambient ionization technology, but its application in quantitative analysis is limited due to its poor sampling stability. Previously, we developed a new micro-pen electrospray ionization tandem mass spectrometry (μPen-ESI-MS/MS) method based on PESI. In this study, a μPen-ESI-MS/MS method to measure testosterone and dextromethorphan in liver microsome samples was developed and validated to further applicate in evaluating drug metabolism stability and CYP450 enzyme activity. A μPen-ESI-MS/MS method for detecting the CYP3A4 substrate testosterone and CYP2D6 substrate dextromethorphan in the liver microsome incubation system were developed, and the linearity, precision and accuracy of the method was validated. The validated method was further used to detect the metabolic stability of testosterone in the liver microsome incubation system. The results showed that the μPen-ESI-MS/MS had high efficiency with 0.3 min spraying time of each sample. The standard curve of the testosterone and dextromethorphan has good linearity (R2 > 0.99), the intra- and inter-batch accuracy of testosterone and dextromethorphan was 95.9%-109.3% and 90.5%-107.3%, respectively; the intra- and inter-batch precision was acceptable with RSD values of 2.4%-13.5% and 3.4%-12.1%. The half-lives of testosterone and dextromethorphan in the liver microsome incubation system were 12 min and 14 min, respectively. This study provided a rapid and sensitive μPen-ESI-MS/MS method for the assay of testosterone and dextromethorphan in liver microsome samples, and provided a new strategy for the evaluation of drug metabolism stability and CYP3A4/CYP2D6 activity.

3.
Acta Pharmaceutica Sinica ; (12): 1263-1272, 2022.
Article in Chinese | WPRIM | ID: wpr-924738

ABSTRACT

Berberine is a naturally occurring benzylisoquinoline alkaloid with a wide range of pharmacological activities, such as antibacterial, anticancer, hypolipidemic, antidiabetic and antidiarrheal. Although berberine has a wide range of curative effects, the extremely low bioavailability (< 1%) limits its clinical application. Pure berberine preparations have not yet been approved for any specific disease. The low oral bioavailability of berberine is mainly due to poor solubility caused by self-aggregation under acidic conditions, low permeability, P-glycoprotein (P-gp)-mediated efflux, and liver and intestine metabolism. To improve the oral bioavailability of berberine, researchers have adopted a variety of strategies, including the application of various nano-delivery systems, penetration enhancers and P-gp inhibitors, structural modifications, and development of berberine derivatives. Improving the oral bioavailability of berberine can improve the pharmacological activity of berberine, reduce the dosage, and then reduce the toxic and side effects. This review summarized the various pharmacological activities, metabolism progress and pharmacokinetic characteristics of berberine, the newly discovered berberine target intestinal microbiota and focused on the strategies to improve the oral bioavailability of berberine by improving solubility and permeability, inhibiting P-gp efflux, and structural modification. The research on berberine was prospected, which provided guidance for the in-depth study of berberine.

4.
Article in Chinese | WPRIM | ID: wpr-880081

ABSTRACT

OBJECTIVE@#To investigate the mutational spectrum and its prognostic significance in patients with acute myeloid leukemia (AML).@*METHODS@#The clinical data of 93 patients with newly diagnosed AML who underwent gene mutation detection by high-throughput sequencing (HTS) from March 2014 to April 2018 in our hospital was analyzed retrospectively. The distribution of mutant genes was summarized and the prognostic factors for intermediate-risk acute myeloid leukemia (IR-AML) were analyzed.@*RESULTS@#Among 93 AML patients, 88.17% had at least one gene mutation, and 53.76% patients showed more than one recurrent genetic mutation. CEBPA showed the highest mutation frequency (20.4%), followed by ASXL1, TET2, NRAS, FLT3-ITD, NPM1, IDH2, DNMT3A, and their mutation frequency were higher than 10%. IDH1/2 and NPM1, ASXL1 and U2AF1, FLT3 and NPM1 often co-occured (P 100×10@*CONCLUSION@#There are co-occurring mutation patterns between the mutated genes. IDH2 mutations relates with poor prognosis and possesses potential to be molecules for model of IR-AML prognostic stratification. Genetic testing based on HTS contributes to revealing the pathogenic mechanism of AML, and is significant for evaluating the prognosis of patients with AML.


Subject(s)
High-Throughput Nucleotide Sequencing , Humans , Leukemia, Myeloid, Acute/genetics , Middle Aged , Mutation , Prognosis , Retrospective Studies
5.
Article in Chinese | WPRIM | ID: wpr-817716

ABSTRACT

@#【Objective】 To explore the attention and its relationship with anxiety in patients with glaucoma.【Methods】A prospective case-control study was done on 20 primary open angle glaucoma(POAG)patients and 28 normal subjects. Response time with and without disturbance was examined by using Matlab software. Anxiety was assessed with State Anxiety Inventory(S- AI)and Trait Anxiety Inventory(T- AI). Response time and inhibiting effects between two groups were compared by t test and Mann- Whitney U test ,respectively. Spearman correlation analysis was applied to determine the relationship between inhibiting effect and anxiety.【Results】The scores of S-AI and T-AI in POAG group were significantly higher than those in normal subjects(t = -3.912,P = 0.001;t = -2.127,P = 0.042). There were statistically significant differences in the scores between groups even after gender was adjusted by using line regression model(β = 9.449,P = 0.000;β = 7.089,P = 0.018). The average response time in POAG group(0.84±0.15)s was significantly longer than that in normal participants(0.75±0.14)s(t = -2.127,P = 0.039). The difference was statistically insignificant when gender was adjusted by using line regression model(β = 0.063,P = 0.210). The inhibiting effect in POAG group(0.126±0.089)s was longer than that in normal subjects(0.085±0.066)s(Z = -1.715,P = 0.086). The inhabiting effect wasn′ t statistically significantly correlated with scores of S- AI and T- AI(P>0.05).【Conclusions】Patients with POAG showed severer anxiety than normal subjects but attention was comparable. There was no significant correlation between attention and anxiety.

6.
Article in Chinese | WPRIM | ID: wpr-871174

ABSTRACT

Objective:To observe the effect of respiratory muscle training on autonomic function and the emotional state of persons with post-stroke depression (PSD).Methods:Fifty-two PSD patients were randomly divided into an observation group and a control group, each of 26. Both groups were given routine drug therapy, physical agents therapy and comprehensive training for hemiplegia, but the observation group additionally received progressive inspiratory muscle resistance training and respiration control training twice a day. Before and after four weeks of treatment, the autonomic functioning, emotional state and the ability in the activities of daily living (ADL) in both groups was evaluated using dynamic electrocardiography, the Hamilton Depression Scale (HAMD) and the modified Barthel index (MBI).Results:After the treatment, the average heart rate variability index in the observation group, the standard deviation of 24-hour R-R intervals(SDNN), the root mean square of successive differences of R-R intervals (RMSSD)and percentage of NN interval deviations greater than 50ms and the high-frequency power (HF) were all significantly higher than before the treatment and significantly higher than the control group′s averages. The average HAMD and MBI scores of the observation group were also significantly better.Conclusion:Breathing training can usefully supplement traditional rehabilitation interventions to alleviate post-stroke depression. It will also improve their autonomic nerve function and ADL ability. Such combined treatment is worthy of promotion and wider clinical application.

7.
Article in English | WPRIM | ID: wpr-829010

ABSTRACT

Objectives@#This paper aimed to investigate the prevalence of diabetes mellitus (DM) and explore the associated risk factors in a very elderly southwest Chinese population.@*Methods@#From September 2015 to June 2016, a cross-sectional survey was conducted to obtain a representative sample of 1,326 participants over 80 years old living in Chengdu. The presence of DM was based on fasting plasma glucose (FPG) and 2-h plasma glucose (2-hPG) levels during an oral glucose tolerance test (OGTT). A logistic regression model was used to calculate the odds ratios ( s) and 95% confidence intervals ( s) of the potential associated factors.@*Results@#The participants' mean age was 83.5 ± 3.1 years. The overall prevalence of DM was 27.4%. The prevalence was higher in males (30.2%) than females (24.7%) ( = 0.02). The prevalence of DM increased with body mass index (BMI) and decreased with aging. The multivariate analysis suggested that male sex ( = 1.433; 95% , 1.116-1.843), hypertension ( = 1.439; 95% , 1.079-1.936), overweight or obesity ( = 1.371; 95% , 1.023-1.834), high heart rate (≥ 75 beats/min; = 1.362; 95% , 1.063-1.746), and abdominal obesity ( = 1.615; 95% , 1.216-2.149) were all significantly positively correlated with DM. However, age was negatively correlated with DM ( = 0.952; 95% , 0.916-0.989).@*Conclusions@#The prevalence of DM and newly diagnosed DM in a very elderly southwest Chinese population was high. OGTT screening should be performed regularly in people aged ≥ 80 years to ensure timely diagnosis of DM.


Subject(s)
Aged, 80 and over , China , Epidemiology , Cross-Sectional Studies , Diabetes Mellitus , Epidemiology , Female , Humans , Male , Prevalence , Risk Factors
8.
Article in Chinese | WPRIM | ID: wpr-787741

ABSTRACT

To evaluate the exported risk of novel coronavirus pneumonia (NCP) from Hubei Province and the imported risk in various provinces across China. Data of reported NCP cases and Baidu Migration Indexin all provinces of the country as of February 14, 2020 were collected. The correlation analysis between cumulative number of reported cases and the migration index from Hubei was performed, and the imported risks from Hubei to different provinces across China were further evaluated. A total of 49 970 confirmed cases were reported nationwide, of which 37 884 were in Hubei Province. The average daily migration index from Hubei to other provinces was 312.09, Wuhan and other cities in Hubei were 117.95 and 194.16, respectively. The cumulative NCP cases of provinces was positively correlated with the migration index derived from Hubei province, also in Wuhan and other cities in Hubei, with correlation coefficients of 0.84, 0.84, and 0.81. In linear model, population migration from Hubei Province, Wuhan and other cities in Hubei account for 71.2%, 70.1%, and 66.3% of the variation, respectively. The period of high exported risk from Hubei occurred before January 27, of which the risks before January 23 mainly came from Wuhan, and then mainly from other cities in Hubei. Hunan Province, Henan Province and Guangdong Province ranked the top three in terms of cumulative imported risk (the cumulative risk indices were 58.61, 54.75 and 49.62 respectively). The epidemic in each province was mainly caused by the importation of Hubei Province. Taking measures such as restricting the migration of population in Hubei Province and strengthening quarantine measures for immigrants from Hubei Province may greatly reduce the risk of continued spread of the epidemic.

9.
International Eye Science ; (12): 1231-1235, 2020.
Article in Chinese | WPRIM | ID: wpr-822249

ABSTRACT

@#AIM: To investigate the prevalence of poor vision and ametropia and main influencing factors of myopia of primary and secondary school students in Xiangtan, providing scientific basis for prevention and control of myopia among primary and secondary school students.<p>METHODS:Cluster random sampling was performed. 6 164 samples were randomly chosen to be tested for uncorrected visual acuity, corrected visual acuity and eye position, and among those whose uncorrected far visual acuity of either eyes below 5.0, refractive power of both eyes were further tested. Self-questionnaire was designed to understand the influencing factors of myopia. <p>RESULTS: Among 6 164 samples, the rate of presenting visual acuity below 5.0 is 40.3%(1 249/3 099)for male and 43.8%(1 343/3 065)for female respectively(<i>P</i><0.01). The prevalence of myopia is 59.1% for male and 68.7% for female respectively(<i>P</i><0.01). The prevalence of myopia is 44.1% for primary school students and 71.3% for secondary school students; the prevalence of hyperopia is 3% for primary school students and 1.7% for secondary school students. Multivariate regression analysis showed that, homework time(>2h), average sleeping time(≤8h)and myopic parents are independent risk factors(<i>OR</i>=1.48, 1.55, 1.26, 1.58, 2.13,all <i>P</i><0.05)for myopia of primary and secondary school students.<p>CONCLUSION: The prevalence of visual impairment and myopia for primary and secondary school students in Xiangtan are high, which is influenced by many factors, by which should be intervened comprehensively.

10.
Article in Chinese | WPRIM | ID: wpr-905392

ABSTRACT

Executive dysfunction is common in stroke patients. Other than the classical assessment tools, there are performance-based assessments for post-stroke executive dysfunction. The related factors include age, infarct region and area, cardiovascular risk factors (hypertension, diabetes, atrial fibrillation and smoking) and education level. In addition to traditional exercise and cognitive rehabilitation, non-invasive brain stimulation technology (transcranial direct current stimulation and transcranial magnetic stimulation), virtual reality and antidepressant have been used more and more for post-stroke executive dysfunction.

11.
Chinese Medical Journal ; (24): 2934-2940, 2019.
Article in English | WPRIM | ID: wpr-781749

ABSTRACT

BACKGROUND@#The pathogenesis of multiple sclerosis (MS) is mediated primarily by T cells, but most studies of MS and its animal model, experimental autoimmune encephalomyelitis (EAE), have focused on CD4 T cells. The aims of the current study were to determine the pathological interrelationship between CD4 and CD8 autoreactive T cells in MS/EAE.@*METHODS@#Female C57BL/6 mice (n = 20) were induced by myelin oligodendrocyte glycoprotein (MOG)35-55 peptide. At 14 days after immunization, T cells were isolated from the spleen and purified as CD4 and CD8 T cells by using CD4 and CD8 isolation kits, and then the purity was determined by flow cytometric analysis. These cells were stimulated by MOG35-55 peptide and applied to proliferation assays. The interferon-gamma (IFN-γ) and interleukin (IL)-4 secretion of supernatant of cultured CD4 and CD8 T cells were measured by enzyme-linked immunosorbent assays (ELISA). For adoptive transfer, recipient mice were injected with MOG35-55-specific CD8 or CD4 T cells. EAE clinical course was measured by EAE score at 0-5 scale and spinal cord was examined by staining with hematoxylin and eosin and Luxol fast blue staining.@*RESULTS@#CD8CD3 and CD4CD3 cells were 86% and 94% pure of total CD3 cells after CD8/CD4 bead enrichment, respectively. These cells were stimulated by MOG35-55 peptide and applied to proliferation assays. Although the CD8 T cells had a generally lower response to MOG35-55 than CD4 T cells, the response of CD8 T cells was not always dependent on CD4. CD8 T cell secreted less IFN-γ and IL-4 compared with CD4 T cells. EAE was induced in wildtype B6 naïve mice by adoptive transfer of MOG35-55-specific T cells from B6 active-induced EAE (aEAE) mice. A similar EAE score and slight inflammation and demyelination were found in naive B6 mice after transferring of CD8 T cells from immunized B6 mice compared with transfer of CD4 T cells.@*CONCLUSION@#Our data suggest that CD8 autoreactive T cells in EAE have a lower encephalitogenic function but are unique and independent on pathogenic of EAE rather than their CD4 counterparts.

12.
Article in Chinese | WPRIM | ID: wpr-816527

ABSTRACT

Autologous breast reconstruction after radical resection of breast cancer has been widely used in clinic.Some surgical methods of autologous breast reconstruction may cause abdominal wall defect to a certain extent.The abdominal wall defect leads to the loss of normal function,which leads to a series of serious pathophysiological changes.At present,transverse rectus abdominis myocutaneous flap(TRAM) and deep inferior epigastric artery perforator flap(DIEP) are commonly used for breast reconstruction.According to the different types of abdominal wall defect after breast autoreconstruction for breast cancer surgery,the corresponding surgical repair scheme should be formulated on the basis of strictly following the basic principles of abdominal wall repair.At the same time,appropriate materials for repairing abdominal wall defect are selected according to the different extent of abdominal wall defect.The posterior sheath and peritoneum of rectus abdominis are usually intact after breast reconstruction,and the anterior sheath of rectus abdominis may be retained.The different layers of abdominal wall can be repaired by surgical methods,and the application of repair materials is emphasized.

13.
Article in Chinese | WPRIM | ID: wpr-816468

ABSTRACT

OBJECTIVE: To analyze the application value of biological mesh in inguinal hernia patients aged from 18 to44 years old. METHODS: The clinical data of 127 patients with inguinal hernia admitted from January 2013 to December 2018 in Huadong Hospital Affiliated to Fudan University were analyzed retrospectively. Through the abdominal transverse fascia reinforcement with biological mesh(tTRB), it was to observe indicators during perioperative and followup periods, to explore the application value and treatment experience of biological mesh in young patients with inguinal hernia. RESULTS: All the 127 cases were successfully completed. There were obvious pain in 3 cases and scrotal edema in 3 cases. No other patients were found to have obvious discomfort. All patients had no complications such as recurrence, chronic pain and infection after 3 to 12 months of follow-up. CONCLUSION: The rational use of biological mesh to repair young and middle-aged patients with inguinal hernia is effective. The advantage is that the effect of mesh minimize the impact on long-term quality of life in young patients. At the same time, the risk of postoperative infection and chronic pain is effectively reduced by reinforcement of transverse fascia with biological mesh, which is worthy of further study.

14.
Article in Chinese | WPRIM | ID: wpr-816459

ABSTRACT

There is no authoritative definition of adolescents in the world which is generally believed that the age ranges from 10 to 19. At present, there are few surveys on the prevalence of inguinal hernia among adolescents at home and abroad. Adolescents are in the process of vigorous growth and development, and the diagnosis and treatment of inguinal hernia is special. There is still much controversy in the treatment of inguinal hernia. In view of the special group of inguinal hernia patients, adolescents should formulate corresponding treatment plan according to their specific characteristics. Detailed preoperative evaluation should be done to find out if there were any special cases(such as hydrocele of testis and cryptorchidism). According to preoperative evaluation, reasonable surgical methods and repairing materials should be selected, carefully operate during surgery, and protect spermatic cord vessels and vas deferens to the maximum extent to avoid adverse complications. Tissue repair or patch reinforcement should be considered according to the degree of posterior inguinal canal defect.

15.
Article in Chinese | WPRIM | ID: wpr-796823

ABSTRACT

Objective@#To observe any curative effect on dysphagia of freehand respiratory training applied to hemiplegic stroke survivors.@*Methods@#Forty-eight hospitalized, hemiplegic stroke survivors were randomly divided into an observation group and a control group, each of 24. Both groups received routine stroke rehabilitation, including motor function training, exercise training, physical therapy and swallowing training. The observation group was additionally provided with barehanded breathing function training, mainly consisting of cough glottis exercise, lip constriction aspiration exercise, chest movement exercise, abdominal muscle contraction and relaxation exercise, as well as abdominal aspiration training. The intervention lasted for 6 weeks for both groups. Before and after the intervention the maximum breathing time, first and second forced volume (FEV1), forced vital capacity (FVC), maximum inspiratory pressure (PImax), standardized swallowing assessment (SSA), Kubota drinking water test (KDWT) and functional dysphagia scale (FDS) scoring were quantified for both groups.@*Results@#There was no significant difference between the two groups′ averages on any of the measurements before the intervention. Afterward, however, the average maximal breathing time, FEV1, FVC, and PImax of the observation group were all significantly higher than before the intervention and significantly higher than those of the control group. After treatment, the observation group′s average KDWT efficiency was not, however, significantly better than that of the control group. Moreover, after the treatment the average SSA and FDS of the observation group were significantly lower than those of the control group, as well as significantly better than before the treatment.@*Conclusion@#Barehanded breathing function training can supplement routine rehabilitation to significantly improve the breathing of stroke survivors.

16.
Article in Chinese | WPRIM | ID: wpr-792001

ABSTRACT

Objective To observe any curative effect on dysphagia of freehand respiratory training applied to hemiplegic stroke survivors. Methods Forty-eight hospitalized, hemiplegic stroke survivors were randomly divid-ed into an observation group and a control group, each of 24. Both groups received routine stroke rehabilitation, in-cluding motor function training, exercise training, physical therapy and swallowing training. The observation group was additionally provided with barehanded breathing function training, mainly consisting of cough glottis exercise, lip constriction aspiration exercise, chest movement exercise, abdominal muscle contraction and relaxation exercise, as well as abdominal aspiration training. The intervention lasted for 6 weeks for both groups. Before and after the inter-vention the maximum breathing time, first and second forced volume ( FEV1 ) , forced vital capacity ( FVC) , maxi-mum inspiratory pressure ( PImax ) , standardized swallowing assessment ( SSA ) , Kubota drinking water test (KDWT) and functional dysphagia scale (FDS) scoring were quantified for both groups. Results There was no significant difference between the two groups' averages on any of the measurements before the intervention. Afterward, however, the average maximal breathing time, FEV1 , FVC, and PImax of the observation group were all significantly higher than before the intervention and significantly higher than those of the control group. After treatment, the obser-vation group's average KDWT efficiency was not, however, significantly better than that of the control group. Moreo-ver, after the treatment the average SSA and FDS of the observation group were significantly lower than those of the control group, as well as significantly better than before the treatment. Conclusion Barehanded breathing function training can supplement routine rehabilitation to significantly improve the breathing of stroke survivors.

17.
Article in Chinese | WPRIM | ID: wpr-849859

ABSTRACT

Objective To explore the experience in the perioperative treatment for osteoporotic fracture complicated with chronic renal insufficiency and its influence on the prognosis of the patients. Methods Sixty-nine patients with osteoporotic fracture complicated with chronic renal insufficiency were retrospectively analyzed. All the patients received surgery and were treated by team during the perioperative period. The comprehensive evaluation for treatment, preoperative retrospective analysis of the disease and treatment, and postoperative follow-up were carried out. Follow-up guidance, and the patient's perioperative renal function, anemia, wound conditions and fracture prognosis were analyzed and followed up. Results Among the 69 patients, there were 27 males and 42 females with an average age of (72.5±14.0). And there were 9 cases of spinal compression fracture, 39 hip fracture, 3 distal radius fracture, 7 proximal humerus fracture and 11 other limb fracture. There were 66 cases of chronic nephrogenic renal insufficiency, 1 case of pre-renal chronic renal insufficiency and 2 cases of post-renal chronic renal insufficiency. The mean preoperative hemoglobin content was (102.5±20.2)g/L, total protein (65.1±7.8)g/L, albumin (36.5±5.4)g/L, serum potassium (4.5±0.8)mmol/L, creatinine (293.5±267.8)μmol/L and glomerular filtration rate (GFR) (41.5±84.0)ml/min in the 69 patients. Towards chronic kidney disease (CKD), 11 cases were in stage II, 27 cases were in stage III, 9 in stage IV and 22 in stage V. All the wounds had first intention healing after the operation. Sixty-two patients were followed up for (62.3±44.8) months. Ten patients died by the end of the follow-up. Nine of them were hip fracture patients. At the end of follow-up, the mortality rate was 16.1%. Conclusions The predilection sites of osteoporotic fracture complicated with chronic renal insufficiency are the hip and spine. Hip fracture is still the main disease affecting the prognosis of the patients. The controlling primary diseases by means of medical and surgical co-management is the premise to ensure the perioperative safety of the patients, but chronic renal insufficiency may still affect the patients' rehabilitation and prognosis after discharge.

18.
Article in Chinese | WPRIM | ID: wpr-818991

ABSTRACT

Objective To understand the laboratory technicians’ abilities in blood slide making and reading in 10 prefectures of Yunnan Province which have passed the provincial malaria elimination evaluation, so as to provide the evidence for improving the malaria elimination surveillance and parasite examination. Methods Thirty negative blood slides were randomly sampled to evaluate coating, dyeing and clean quality and reading results, and 4 laboratory technicians were sampled to evaluate their reading abilities from each prefecture level and its 2 subordinate counties (districts) respectively, and then the results were analyzed. Results A total of 869 negative blood samples were evaluated. The coincidence rate was 100%. The proportions of good coating, dyeing and clean quality were 96.09%, 91.71% and 96.89%, respectively. Totally 576 blood slides were used to evaluate the reading ability. The number of correct reading was 505, and the correct rate was 87.67%. Among them, the Plasmodium vivax correct reading rate was 87.76%, the P. falciparum correct reading rate was 87.50%, and the correct reading rate of mixed infections was 47.62%. The laboratory technicians’ ability to the mixed infections was significantly lower than the ability to the others (χ2 = 37.169, P < 0.05), however, in the laboratory technicians’ abilities, there was no significant difference among the center (s) for disease control and prevention, general hospitals and township hospitals (χ2 = 2.782, P > 0.05), and the prefecture, county and township levels (χ2 = 0.358, P > 0.05) . Conclusion The 10 prefectures have passed the provincial evaluation in blood slide making and microscopic examination skill indicators requested, but the medical and public health institutions at all levels still should further improve their laboratory technicians’ abilities in blood slide making and microscopic examination skills.

19.
Article in Chinese | WPRIM | ID: wpr-818539

ABSTRACT

Objective To understand the laboratory technicians’ abilities in blood slide making and reading in 10 prefectures of Yunnan Province which have passed the provincial malaria elimination evaluation, so as to provide the evidence for improving the malaria elimination surveillance and parasite examination. Methods Thirty negative blood slides were randomly sampled to evaluate coating, dyeing and clean quality and reading results, and 4 laboratory technicians were sampled to evaluate their reading abilities from each prefecture level and its 2 subordinate counties (districts) respectively, and then the results were analyzed. Results A total of 869 negative blood samples were evaluated. The coincidence rate was 100%. The proportions of good coating, dyeing and clean quality were 96.09%, 91.71% and 96.89%, respectively. Totally 576 blood slides were used to evaluate the reading ability. The number of correct reading was 505, and the correct rate was 87.67%. Among them, the Plasmodium vivax correct reading rate was 87.76%, the P. falciparum correct reading rate was 87.50%, and the correct reading rate of mixed infections was 47.62%. The laboratory technicians’ ability to the mixed infections was significantly lower than the ability to the others (χ2 = 37.169, P < 0.05), however, in the laboratory technicians’ abilities, there was no significant difference among the center (s) for disease control and prevention, general hospitals and township hospitals (χ2 = 2.782, P > 0.05), and the prefecture, county and township levels (χ2 = 0.358, P > 0.05) . Conclusion The 10 prefectures have passed the provincial evaluation in blood slide making and microscopic examination skill indicators requested, but the medical and public health institutions at all levels still should further improve their laboratory technicians’ abilities in blood slide making and microscopic examination skills.

20.
International Eye Science ; (12): 113-117, 2019.
Article in Chinese | WPRIM | ID: wpr-688275

ABSTRACT

@#AIM: To evaluate the agreement and interchangeability of a Scheimpflug camera(Pentacam)and a partial coherence interferometry keratometer(IOL Master)in eyes having preoperative assessment for routine cataract surgery. <p>METHODS: In this prospective clinical study, 41 patients(64 eyes)with normal cornea from January 2017 to June 2017 in our hospital were enrolled. Simulated K, true net power and Holladay equivalent K(EKR)measured at 1.0 to 7.0 mm corneal diameters were measured by the Pentacam system and mean keratometer(Km)was measured by the IOL Master. Paired samples <i>t</i> test, Pearson correlation analysis and Bland-Altman method were used for statistical analysis. <p>RESULTS: Among patients with cataract of normal cornea, the mean Scheimpflug K for true net power and EKR at 1.0mm, 2.0mm, 3.0mm corneal diameters were statistically significantly less than the mean Km(IOL Master). The EKR at 4.5mm, 5.0mm, 6.0mm, 7.0mm of corneal diameters were significantly greater than the mean Km(IOL Master)(<i>P</i><0.01 for all). The difference between Sim K and EKR at 4.0mm measured by Pentacam and Km measured by IOL Master was the smallest(-0.03D±0.252D and 0.04D±0.244D, respectively; <i>t</i>=-1.018, <i>P</i>=0.313; <i>t</i>=1.461, <i>P</i>=0.149), and it had the best uniformity with 95% credible interval of -0.53D to 0.46D(sim K)and -0.43D to 0.52D(equivalent K at 4.0mm). A Pearson correlation revealed a high correlation for corneal power measurements between the two devices(<i>r</i>>0.9 for all, <i>P</i><0.01 for all).<p>CONCLUSION: The sim K and EKR at 4.0mm had the closest match with the Km(IOL Master)for Chinese patients with cataract of normal cornea. However, the application of these devices should be combined with clinical features.

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