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1.
Article in Chinese | WPRIM | ID: wpr-843215

ABSTRACT

Objective : To investigate the effect of mechanistic target of rapamycin complex 1 (mTORC1) on group 3 innate lymphoid cells (ILC3) function. Methods ¡¤ Intestinal lamina propria leukocytes (LPL) of C57BL/6 wild type mice were stimulated by rapamycin, the specific inhibitor of mTORC1 signaling pathway, in vitro, and then quantity and function of ILC3 were detected by flow cytometry. Next, purified ILC3 from mice intestinal LPL were sorted by flow cytometry. After the activation of ILC3 with IL-23, mRNA expression levels of Rorc (the gene encoding retinoic acid receptor related orphan receptor, i.e. RORγt), Il22 and Rptor (the gene encoding key component protein of mTORC1, i.e. Raptor) were detected by real-time qPCR. For further study, a genetically engineered mouse model specifically knocked out Raptor in ILC3 was constructed. Effects of mTORC1 loss on the quantity and function of ILC3 as well as gut structure were detected by flow cytometry, real-time qPCR and hematoxylin-eosin staining. Results ¡¤ The total ILC3 number had no change, but the secretion of IL-22 by ILC3 reduced after stim-ulation with rapamycin. Il22, Rorc and Rptor mRNA expression levels were upregulated simultaneously in ILC3 after activation with IL-23. In addition, there was no significant difference in the numbers and proportions of total ILC3 and ILC3 subsets as well as gut structure in Rap-tor-deficient mice, but the cytokine IL-22 secretion level of ILC3 significantly decreased in these mice. Conclusion ¡¤ Loss of mTORC1 func-tion inhibits ILC3 from secreting IL-22 but has no effect on the intestinal structure and intestinal ILC3 development, which reveals the positive regulation of mTORC1 signaling on intestinal ILC3 function.

2.
Article in English | WPRIM | ID: wpr-776893

ABSTRACT

Resistance to cisplatin (DDP)-based chemotherapy is a major cause of treatment failure in human gastric cancer (GC). It is necessary to identify the drugs to re-sensitize GC cells to DDP. In our previous research, Zuo Jin Wan Formula (ZJW) has been proved could increase the mitochondrial apoptosis via cofilin-1 in a immortalized cell line, SGC-7901/DDP. Due to the immortalized cells may still difficult highly recapitulate the important molecular events in vivo, primary GC cells model derived from clinical patient was constructed in the present study to further evaluate the effect of ZJW and the underlying molecular mechanism. Immunofluorescent staining was used to indentify primary cultured human GC cells. Western blotting was carried out to detect the protein expression. Cell Counting Kit-8 (CCK-8) was used to evaluate cell proliferation. Flow cytometry analysis was performed to assess cell apoptosis. ZJW inhibited proliferation and induced apoptosis in primary DDP-resistant GC cells. Notably, the apoptosis in GC cells was mediated by inducing cofilin-1 mitochondrial translocation, down-regulating Bcl-2 and up-regulating Bax expression. Surprisingly, the level of p-AKT protein was higher in DDP-resistant GC cells than that of the DDP-sensitive GC cells, and the activation of AKT could attenuate ZJW-induced sensitivity to DDP. These data revealed that ZJW can increase the chemosensitivity in DDP-resistant primary GC cells by inducing mitochondrial apoptosis and AKT inactivation. The combining chemotherapy with ZJW may be an effective therapeutic strategy for GC chemoresistance patients.


Subject(s)
Adult , Aged , Aged, 80 and over , Apoptosis , Cell Proliferation , Cisplatin , Pharmacology , Therapeutic Uses , Cofilin 1 , Metabolism , Drug Resistance, Neoplasm , Drugs, Chinese Herbal , Pharmacology , Female , Humans , Male , Middle Aged , Mitochondria , Metabolism , Pathology , Proto-Oncogene Proteins c-akt , Metabolism , Proto-Oncogene Proteins c-bcl-2 , Metabolism , Stomach Neoplasms , Drug Therapy , Metabolism , Pathology , Tumor Cells, Cultured
3.
Article in Chinese | WPRIM | ID: wpr-857312

ABSTRACT

Aim To investigate whether paeoniflorin ( PF) inhibits the activation of RAW 264. 7 macropha-ges induced by high glucose (HG) via JAK2/STAT3 signaling pathway. Methods RAW264. 7 macrophages were cultured in vitro. HG was used as the stimulator. PF and JAK2/STAT3 gene silencing interfered with macrophage activation. All cells were divided into nine groups; LG, HG, HG + PF, LG + JAK2 siRNA, JAK2 siRNA + HG, JAK2 siRNA + HG + PF, LG + STAT3 siRNA,STAT3 siRNA + HG,STAT3 siRNA + HG + PF. Macrophage proliferation, chemotaxis, expression and secretion of inflammatory cytokines, J A K2 and STAT3 protein expression and the phosphorylation levels were detected respectively. Results Compared with LG group,in HG group,the macrophages' chcmo- taclic function significantly increased {P <0. 05) ,the mRNA expression of iNOS,TNF-a, IL-lp and MCP-1 and the secretion of inflammatory cytokines (TNF-a, IL-IP,MCP-1) in cell culture medium were markedly elevated ( P < 0. 05 , P < 0. 01 ) , and the phosphorylation of JAK2 and STAT3 protein significantly increased ( P < 0. 05 ). JAK2 and STAT3 gene silencing could suppress the HG-induced the mRNA expression of iN- OS,TNF-a, 1L-IP and MCP-1 and the secretion inflammatory cytokines in cell culture medium as well as the phosphorylation of JAK2 and STA'13 protein ( P < 0. 05,/' <0. 01 ). PF could significantly inhibit HG-induced macrophage chemokine and migration,inflammatory cytokine production and secretion, and JAK2, STAT3 protein phosphorylation (/'<0. 01). Conclusion HG can stimulate macrophage activation by inducing JAK2/STAT3 signaling pathway,and PF inhibits the activation of Raw 264. 7 macrophages induced by high glucose via JAK2/STAT3 pathway suppression.

4.
Article in Chinese | WPRIM | ID: wpr-824517

ABSTRACT

Liver ischemia-reperfusion injury(IRI)is a major complication of hemorrhagic shock,liver transplantation.and other liver surgeries.It's important to study the targets towards liver IRI for preventing and mitigating the clinical renal injury.It has been reported that the peroxisome proliferator activated receptor gamma(PPARγ)protects the liver against IRI by targeting family with sequence similarity 3 member A(FAM3 A).At the meantime,noncoding RNAs,including lncRNAs and miRNAs,have also been reported to play important roles on the process of hepatic IRI.This review briefly discussed the roles and mechanisms of PPAR3,,FAM3A and noncoding RNAs in liver IRI,to find potential targets of gene ther-apy,aiming to prevent and mitigate the liver IRI as well as to improve postoperative liver function.

5.
Journal of Preventive Medicine ; (12): 163-166, 2018.
Article in Chinese | WPRIM | ID: wpr-792713

ABSTRACT

Objective To evaluate the clinical nursing pathway in the treatment of stroke patients in combination with traditional Chinese medicine and western medicine. Methods A hundred patients were randomly divided into observation group and control group, 50 cases of each, which selected in Zhoushan Hospital of Traditional Chinese Medicine from January to December in 2015. The normal nursing methods were used in the control group and the clinical nursing paths were used in the observation group. Results The average value of Barthel score of the observation group was significantly better than that of the control group (55.93±9.12 vs. 46.51±9.29, P<0.05) . The recovery efficiency of the observation group was significantly higher than that of the control group (90.00% vs. 62.00%, P<0.05) . The score of satisfaction of nursing work in the observation group was higher than that of the control group (9.13±0.49 vs.7.08±0.72, P<0.05), and the average length of stay was shortened (43.62±3.79 vs. 65.13±3.11, P<0.05) respectively compared with the control group, and the curative effect of topsis was evaluated by topsis method, and the Ci value of the observation group (Ci=0.781) was better than that of the control group (Ci =0.219) . Conclusion The clinical nursing pathway could significantly improve stroke patients Barthel score, muscle strength and nursing job satisfaction, and overall reduce the duration of hospital stays.

6.
Article in Chinese | WPRIM | ID: wpr-485867

ABSTRACT

Objective To investigate and analyze TCM syndrome characteristics, impact factors and internal relationship among the factors of mental fatigue of university students. A preliminary understanding of mental fatigue situation of university students and prevent diseases was acquired in advance. Methods 100 students were randomly selected in Beijing University of Chinese Medicine, of whom 40 were undergraduate students and 60 were post-graduate students. Mental fatigue questionnaire was used to investigate mental fatigue situation for students, and TCM constitution scale was applied to collect TCM syndromes. A body energy survey was also conducted. Results In undergraduate and post-graduate students, the mental fatigue rate was relatively higher, both of them were over 50%of the total numbers, 57.5%(23/40)、61.7%(37/60) respectively. For the fatigue levels distribution in these two groups, the ratios of slight mental fatigue (undergraduate:52.5%;post-graduate:56.67%) were both higher than those of severe mental fatigue (undergraduate:5%;post-graduate:5%). In the undergraduate group, the top three biased constitutions among mental fatigue students were yang deficiency (7 out of 23:30.43%), qi deficiency (3 out of 23:13.04%) and dampness-heat (3 out of 23:13.04%). In the post-graduate group, the top three biased constitutions were yang deficiency (11 out of 37:29.73%), qi deficiency (6 out of 37: 16.22%) and dampness-heat (5 out of 37: 13.51%). Conclusion It is easier for university students with biased constitution to suffer from mental fatigue, mostly with yang deficiency, qi deficiency and dampness-heat constitution.

7.
Journal of Preventive Medicine ; (12): 683-686, 2014.
Article in Chinese | WPRIM | ID: wpr-792318

ABSTRACT

Objective Todescribethemonthlyincidencedistributionofhand-foot-mouthdisease(HFMD)from2010to 2012inZhoushancityandtoanalyzetheseasonaltrendandthecausesofpeakchanges.Methods Circulardistribution method was used to identify the peak period of HFMD incidence.Watson -William test and Watson's U2 test were applied for comparing the peak periods respectively.Chi -square test was conducted to analyze and compare the distribution of HFMDpathogenindifferentyearsandBonferronimethodwasusedformultiplecomparisons.Results Therewasseasonal centralized tendency of HFMD incidence during 2010 to 2012 (P<0.01).The peak period occurred between 8th of May and 1st of September which showed a significant difference among three years (P<0.01).Specifically,the peak period in 2012 was earlier than that in 2010 and 201 1 (both P<0.01 ).Among the 356 reported cases with laboratory confirmed, there was significant difference between the distribution of HFMD pathogen by Chi -square test (P <0.01 ).The predominant strain was EV71 in 2010 and 2011 accounting for 58.46%and 64.62%respectively and other enteric viruses in 2012 accounting for 48.84%.The ratio of pathogens in 2012 was statistically different from that in 2010 and 201 1 respectively(bothP<0.01).Conclusion HFMDcasesoccurwithobviouspeakseason,regionalandgeographic differences.The major pathogens of EV71 in 2010 and 2011 were changed into other enteric viruses in 2012.

8.
Article in Chinese | WPRIM | ID: wpr-440749

ABSTRACT

Objective:To evaluate the efficacy and safety of capecitabine-based regimens in treating patients with liver metastases from breast cancer. Methods:A total of 163 patients with liver metastases from breast cancer who received capecitabine-based regimens between January 1, 2000 and Dec 31, 2011 were retrospectively reviewed. The clinicopathological characteristics and treatment outcomes of these patients were evaluated. Results:Of the 163 patients retrospectively analyzed, 109 received docetaxel plus capecitabine (TX) and 54 received vinorelbine plus capecitabine (NX). TX treatment achieved objective responses in 59 patients (54.1%), including complete response in four patients, partial response in 55 patients, and stable disease in 25 patients. In patients who received NX, the objective response and clinical benefit rates were 50.0%and 70.4%, respectively;one patient had a complete response, 26 patients had a partial response, and 11 patients had a stable disease. The safety profiles of TX treatment were more favorable and predictable compared with NX treatment, with a low incidence of grade 3/4 adverse events in hematological and non-hematological toxicities. Results showed that median overall survival after liver metastases (LMS), progression-free survival (PFS), and post-metastasis survival (MSR) were 26, 8, and 28 months in the TX arm. LMS, PFS, and MSR were longer in the TX arm than in the NX arm. Conclusion:Capecitabine-based regimens showed tolerance in patients with liver metastases from breast cancer. TX treatment had a tendency of lower toxicity and was more effective compared with NX treatment.

9.
Article in Chinese | WPRIM | ID: wpr-438737

ABSTRACT

T-DM1 is a novel antibody-drug conjugate that has similar biological activity with that of trastuzumab. T-DM1 specifically delivers DM1, the effective anti-microtubule drug, into the cytoplasm of tumor cells with HER2 overexpression. The efficacy of T-DM1 monotherapy is better than lapatinib in combination with capecitabine and T-DM and is expected to become the standard second-line treatment for HER2-positive advanced breast cancer drugs. Clinical trials that compare T-DM1 with trastuzumab joint taxane as the first-line of treatment for advanced breast cancer trials are currently being performed. T-DM1 is a brand new anti-HER2 drug after trastuzumab. U.S. FDA already approved T-DM1 as a drug for the treatment of HER2-positive advanced breast cancer patients.

10.
Article in Chinese | WPRIM | ID: wpr-452963

ABSTRACT

Objective Through the two D-dimer content detection of the fracture patients and healthy con-trols,and analyze the difference between them ,to explore the correlation between fractures were detected two D-dimer and the importance and the patient′s condition.Methods 306 of fracture patients were collected for determination of D-dimer content were detected at the same time ,100 healthy people as control two D-dimer content;analysis of the correlation between the fracture in 306 cases patients with D-dimer content .Results The plasma D-dimer level of pa-tients with two fracture(10.7 ±2.6)mg/L,which was significantly higher than that of healthy subjects (0.3 ±0.1) mg/L(t=11.07,P<0.01);The fracture number,fracture patients plasma D-dimer detection results content is high-er,the more serious condition .Conclusion The plasma D-dimer level was significantly higher in patients with two fractures,coagulation Stata ,fibrinolytic enhancement ,easily lead to venous thrombosis and disseminated intravascular coagulation in patients with more complications such as fracture;fracture,the higher the content of plasma D-dimer, patients with more serious ,there is correlation .

11.
Article in Chinese | WPRIM | ID: wpr-336741

ABSTRACT

<p><b>OBJECTIVE</b>To prepare Form A and Form B of benazepril hydrochloride and to compare the differences in spectrums, thermodynamics and crystal structure between two polymorphic forms.</p><p><b>METHODS</b>Form A and Form B of benazepril hydrochloride were characterized by Fourier transform infrared spectroscopy (IR), thermal gravimetric analysis (TG), differential scanning calorimetry (DSC), powder x-ray diffraction (PXRD) and single crystal x-ray diffraction (SCXRD).</p><p><b>RESULTS</b>Preparation method, crystal structure and polymorphic stability of Form A and Form B of benazepril hydrochloride were obtained. Based on the analysis of crystal structure of both polymorphs, Form A belonged to monoclone space group P2(1) with a=7.8655(4)Å, b= 11.7700(6)Å, c= 13.5560(7)Å, β= 102.9470(10)°, V=1223.07 (11)Å(3) and Z=2, while Form B belonged to orthorhombic space group P212121, with a=7.9353(8)Å, b=11.6654(11)Å, c=26.6453(16)Å, V=2466.5(4)Å(3) and Z=4. From the DSC and XRD results, Form B of benazepril hydrochloride could be transformed into Form A after heating treatment.</p><p><b>CONCLUSION</b>Form A and Form B of benazepril hydrochloride are both anhydrous and displayed different polymorphs due to different molecular configuration. Furthermore, Form A exhibits more stable than Form B at high temperatures.</p>


Subject(s)
Benzazepines , Chemistry , Crystallization , Drug Stability , Molecular Conformation
12.
Chinese Journal of Epidemiology ; (12): 1001-1004, 2012.
Article in Chinese | WPRIM | ID: wpr-289596

ABSTRACT

Objective Through reviewing data on surveys,tested materials especially on lead in Zhoushan fisheries,with ground lead,cadmium and methyl-mercury were analyzed and evaluated.Methods According to the distribution of Zhoushan fisheries,we randomly selected a certain number of Zhoushan seafood as research objects from four counties or districts.Different kinds of seafood would include sea fish,seawater crustaceans,seawater soft-bodied animals and sea algae.The inedible parts of all the seafood were removed,and then the samples of the edible parts were grinded into homogenate.We measured the contents of lead and cadmium,using graphite furnace atomic absorption spectrometry method.The content of methylmercury was measured,using the gas chromatography (acid extraction method of mercaptoacetic cotton).Data from 2007 to 2009 was analyzed under SPSS software.Results (1)The average standardized rates were:lead as 3.90% (11/282),cadmium as 11.35% (32/282) and methylmercury as 2.84% (8/282).(2) Results from the comprehensive evaluation on the contents of metals showed the following rankings:seaweed (0.4513 mg/kg),marine molluscs (0.1155 mg/kg),marine crustaceans (0.0486 mg/kg),sea-fish (0.0419 mg/kg).(3) Results from the single-factor variance analysis showed that the accumulation of lead,cadmium and methylmercury in different types of seafood were:lead F=35.683 (P<0.001) ;cadmium F=25.301 (P<0.001) ; methylmercury F=25.990 (P<0.001).(4) Data on the safety analysis related to the different types of seafood on lead,cadmium,methylmercury food,the Chisquare tests showed as:lead x2=10.167,P<0.05 ; cadmium x2=62.940,P<0.001 ; methylmercury x2=20.960,P<0.001.(5) Seafood in different years on lead,cadmium,methylmercury accumulation comparison,the Spearman correlation test results showed:lead P=0.000,cadmium P=0.974,methylmercury P=0.024.(6) The contents of seafood lead,cadmium and methylmercury in different years that with statistically significant differences were as follows:lead x2=6.440,P<0.05; cadmium x2=34.455,P<0.001 ;methylmercury x2=5.226,P>0.05.Conclusion The situation of heavy metal pollution in different kinds of Zhoushan fisheries was different.Algae appeared the worst while sea fish,sea water crustaceans pollution were light,with lead and methylmercury mainly influencing sea algae and cadmium mainly in the sea algae,followed by seawater software class.The rates over standards of different kinds of seafood were different from each other,while the rates over standards of lead,cadmium and methylmercury in sea algae were the highest.Evaluated by pollution index,lead,methylmercury pollution was still low,but cadmium in the sea with mild pollution in algae seafood.In the recent three years,heavy metal lead pollution in seafood were worsened,while the cadmium,methylmercury pollution levels remained basically stable.

13.
Article in Chinese | WPRIM | ID: wpr-326240

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the molecular characteristics and genotype of Japanese encephalitis virus (JEV) found in vector mosquitoes in Zhejiang province from 1982 to 1983.</p><p><b>METHODS</b>A total of 3188 mosquitoes were collected in Dinghai district and Yiwu city in Zhejiang province, during year 1982 and 1983. The virus was isolated by C6/36 cell, and then identified by hemagglutination inhibition test. The isolated strains were activated in year 2011, and plaque forming unit (PFU) were applied to test the virus titer. The suckling rats were tested under intracranial inoculation, where PrM and E genes were amplified and sequenced. Their nucleotide and amino acid sequences were analyzed and compared with the JEV vaccine strain SA14-14-2 and the JEV isolated in Zhejiang province during 2007 and 2010; and phylogenetic tree were constructed by bioinformatic software.</p><p><b>RESULTS</b>From the 3188 mosquitoes captured, eleven virus strains were isolated and found to be able to cause cytopathogenic effect (CPE) in C6/36 cells within 72 hours. Virus titer ranged from 2.5 to 6.47 lg PFU/ml. The suckling rats would die within 72 hours since the inoculation. The phylogenetic analysis with the PrM and E genes showed that the JEV isolated in Zhejiang during 1982 and 1983 belonged to genotype III; while the JEV isolated in Zhejiang during 2007 and 2010 belonged to genotype I. The analysis of E genes from 5 isolated strains found that the homology of nucleotide sequence was over 98.9%, and the homology of amino acid sequence was over 99.8%. The compare between the 5 virus strains and the vaccine strain SA14-14-2 found 10 common amino acid variation sites, and showed that the homology of nucleotide sequence was over 97.7%, and the homology of amino acid sequence was 99.2%. The compare between the 5 virus strains and the JEV isolated in Zhejiang during 2007 and 2010 found a 87.7% - 87.9% nucleotide homology and an over 98.8% amino acid homology.</p><p><b>CONCLUSION</b>The JEV isolated from the mosquitoes in Dinghai district and Yiwu city between year 1982 and 1983, were genotype III.</p>


Subject(s)
Amino Acid Sequence , Animals , China , Culicidae , Virology , Encephalitis Virus, Japanese , Classification , Encephalitis, Japanese , Virology , Genotype , Phylogeny , Rats
14.
Acta Pharmaceutica Sinica ; (12): 253-260, 2011.
Article in Chinese | WPRIM | ID: wpr-348968

ABSTRACT

The NF-kappaB pathway regulates the expression of over 150 target genes, e.g., cytokines, chemokines, leukocyte adhesion molecules and inducible effector enzymes. Consequently, it plays a crucial role in innate and adaptive immune responses, inflammatory response, stress responses, apoptosis and so on. IkappaB kinase (IKK) is the key of this pathway, and it owns a special structure which consists of catalytic subunit and regulatory subunit. Naturally, the activation of IKK needs the interaction of the two subunits and phosphorylation by its upstream kinases. Actually, there are two methods of activation of the NF-kappaB pathway, and both of the methods need the IKK complex. Given to the crucial role of IKK, researchers have isolated and synthesized amounts of IKK inhibitors, and these provide a great convenience to develop novel anti-inflammatory and anti-tumor drugs.


Subject(s)
Animals , Anti-Inflammatory Agents , Pharmacology , Antineoplastic Agents , Pharmacology , Enzyme Activation , Enzyme Inhibitors , Metabolism , Pharmacology , Humans , I-kappa B Kinase , Chemistry , Metabolism , Physiology , NF-kappa B , Metabolism , Phosphorylation , Signal Transduction
15.
Article in Chinese | WPRIM | ID: wpr-746644

ABSTRACT

OBJECTIVE@#To introduce recent progress on diagnosis and treatment of benign symmetric lipomatosis (BSL).@*METHOD@#Detailed clinical data of 6 patients with BSL were reviewed and analyzed. We present a summary of the clinical symptoms, physical sign, diagnosis and therapeutic methods of BSL. And related literatures were discussed together.@*RESULT@#All of 6 patients have excessive subcutaneous fat deposit predominantly around neck. One patients had upper extremity localizations. Six patients had the complication of left ventricular diastolic function changes, glucose intolerance or diabetes mellitus, chronic hepatopathy, hyperuricemia and sleep apnea syndrome in one or more. One patients with several symptoms occur simultaneously, another one female patient was accompanied by all symptoms but chronic hepatopathy. Five male patient were alcohol abusers. Total neck lipectomy and abstinence from alcohol were performed on 5 patients. One patient refused treatment. During a follow-up of 3 months to 4 years, one patients was relapsed again, and no recurrence was seen in another 4 patients. All patho logical results were nonencapsulated fat.@*CONCLUSION@#BSL is a lipodystrophy caused by diffuse fatty tissue, symmetry deposition in the neck and shoulder subcutaneous fascia space or deep fascial space. The highest incidence favors to middle-aged man who is alcoholic. Chronic alcohol addiction and typical clinical symptoms help to diagnosis BSL. Lipectomy represents a successful procedure in treating BSL.


Subject(s)
Adult , Alcoholism , Fascia , Pathology , Female , Humans , Lipectomy , Lipomatosis, Multiple Symmetrical , Diagnosis , General Surgery , Male , Middle Aged , Neck , Pathology , Shoulder , Pathology
16.
Article in Chinese | WPRIM | ID: wpr-748008

ABSTRACT

OBJECTIVE@#To discuss the diagnosis and treatment of child cervical and thoracic angio lymphoma.@*METHOD@#Three cases of huge cervicothoracic angio lymphoma were performed by cervicothoracic approach and median split of sternum under general anesthesia.@*RESULT@#The pathological diagnosis of three cases were angio lymphoma, and there was no recurrence and complication during the follow-up.@*CONCLUSION@#The tumor could be fully exposed by cervicothoracic approach operation, which was the basic guarantee for completely revealed tumor, and no recurrence was found. Other treatment should not be recommend for huge cervicothoracic angio lymphoma.


Subject(s)
Child , Child, Preschool , Female , Humans , Lymphangioma , Diagnosis , General Surgery , Male , Neck , Thoracic Neoplasms , Diagnosis , General Surgery
17.
Article in Chinese | WPRIM | ID: wpr-349909

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the H(2)S pollution in cabins which caused the fishermen's eye burns.</p><p><b>METHODS</b>Fifty-six fishing boats' H(2)S concentration was surveyed and 56 fishermen's eyes were inspected. The air samples were collected from 21 fishing boats' cabins, where the eye burns took place and the monitoring conditions met the inspection requirement, in order to confirm the concentration of H(2)S when eye burns and the systemic poisoning happened. Thirty fishing boats were divided into two groups: one was using air ventilating and spraying, the other was using naturally ventilation to find out the effective method of dispersing H(2)S. Five fishing boats were surveyed in which the fishermen had slight symptom of bulbar conjunctiva hyperemia and cough to find out the minimum concentration of H(2)S which caused the eye burns and respiratory mucosa.</p><p><b>RESULTS</b>Among 56 fishermen who were surveyed, 46 fishermen's eyes (92 eyes) burnt and they were from 21 vessels, 10 of them (20 eyes) were moderate, 36 of them (72 eyes) were light. The concentration of H(2)S in the 21 fishing boats' cabins which caused eye burns was (99 ± 38) mg/m(3). The first measuring of the concentration of H(2)S in the 30 fishing boats in which fish were not discharged yet was (219 ± 31) mg/m(3). Air ventilating and spraying group's concentration of H(2)S was (213 ± 24) mg/m(3), while that of naturally ventilation group's was (225 ± 36) mg/m(3). Dispersing after 1 hour, the concentration of H(2)S of air ventilating and spraying group was (21 ± 3) mg/m(3), the decreased concentration was (192 ± 21) mg/m(3), fell 90%; the concentration of naturally ventilation group was (184 ± 36) mg/m(3), the decreased concentration was (41 ± 8) mg/m(3), fell 18%. The difference between the two groups' decreased concentration was significant (t = 25.627, P < 0.05). The threshold value of H(2)S concentration that could cause the eye burns was 38 mg/m(3)(exposure time 120 min). In 7 vessels, the concentration of H(2)S in the cabins was (123 ± 9) mg/m(3) where 10 fishermen's moderate eye burns happened. In other 7 vessels, the concentration of H(2)S in the cabins was (54 ± 7) mg/m(3) where 19 fishermen's light eye burns happened. The difference of H(2)S concentration between the two groups was significant (t = 14.236, P < 0.05).</p><p><b>CONCLUSION</b>High H(2)S concentration and long exposure time in cabin can cause serious eye burns. The bilge air ventilation and inner cabin spraying are the effective method to clear the H(2)S in cabin within short time.</p>


Subject(s)
Air Pollutants, Occupational , Confined Spaces , Eye Burns , Epidemiology , Fisheries , Humans , Hydrogen Sulfide , Ships
18.
Chinese Journal of Epidemiology ; (12): 1001-1004, 2010.
Article in Chinese | WPRIM | ID: wpr-341015

ABSTRACT

Objective To study the rates of infection and physicochemical characteristics of the third stage Anisakis simplex larvae among marine fish caught in Zhoushan Fishery. Methods Fish were dissected to detect Anisakis larvae and identified morphologically. The survival tolerance of the third stage Anisakis simplex larvae in various medium, anthelmintic drug, temperature were studied in laboratory. Results The total infection rate of Anisakis simplex larvae in fish was 49.10%. High rates of Anisakis infection were observed in hairtails, Pneumatophorus japonicus, Miichthys miiuy,Argyrosomus argentatus and Muraenesox cinereus (infection rates >90 percent). The infection intensity of Anisakis per fish varied from 1 to 114. The mean intensity of Anisakis larvae was 15.20 per fish.3314 Anisakis were detected in 218 marine fish. The survival tolerance of the third stage Anisakis simplex larvae in various Medium, anthelmintic drug, temperature were observed in laboratory condition. The third stage Anisakis simplex larvae showed a strong endurance to stock condiment. The anisakicidal effects of the high purity wine were more effective than that of the low purity wine. The anisakicidal effects of 6.25 g/L mebendazole composite were more effective than that of 18.75 g/L and also more effective than those of other drugs. The third stage Anisakis simplex larvae could survive with length up to 9 h and 12 h in condition of -20 ℃, -10 ℃ and very sensitive to high temperature treatment. However, they could barely survive in more than 11 s and 1 s under the temperature of 50 ℃ and 60 ℃. Conclusion The percentage of infection was fairly high for Anisakis larvae of marine fish caught in Zhoushan Fishery. The third stage Anisakis simplex larvae was shown to have a fairly good tolerance to the external environments. The marine fish were frozen under -20 ℃ beyond 24 h before they were sold on market and cooked with high temperature seemed to be helpful for preventing and controlling effectively the infection of Anisakis.

19.
Article in Chinese | WPRIM | ID: wpr-318283

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the clinical and pathological features, diagnosis and treatment of Castleman disease (CD).</p><p><b>METHODS</b>Clinical features and related information on diagnosis and treatment of eight cases of CD were retrospectively analyzed. The size of involved lymph nodes ranged from (2 cm x 2 cm x 3 cm) - (4 cm x 3 cm x 2 cm). The lymph nodes were found in level I (1 case), level II (3 cases), level III (3 cases) and level IV (1 case). CT examination in eight patients showed the lesions manifested as ellipse soft masses. Dynamic contrast CT scan in four patients showed ring-enhanced area around the masses. Blood routine examination in eight patients were generally normal, with six patients had mild anemia.</p><p><b>RESULTS</b>Based on the clinical classification, all lesions in this group were localized CD. Histopathology indicated that all lesions were of hyaline-vascular type. After surgery, there was no recurrence during the follow-up period.</p><p><b>CONCLUSIONS</b>Patients with localized CD mainly have lymphadenectasis in a single location. The CT scan can give some evidence. Surgery should be given first priority.</p>


Subject(s)
Adolescent , Adult , Aged , Castleman Disease , Diagnosis , Pathology , Female , Humans , Lymph Nodes , Pathology , Male , Middle Aged , Neck , Pathology , Retrospective Studies , Young Adult
20.
Article in Chinese | WPRIM | ID: wpr-433183

ABSTRACT

Objective:To introduce recent progress on diagnosis and treatment of benign symmetric lipomatosis(BSL).Method:Detailed clinical data of 6 patients with BSL were reviewed and analyzed.We present a summary of the clinical symptoms,physical sign,diagnosis and therapeutic methods of BSL. And related literatures were discussed together.Result:All of 6 patients have excessive subcutaneous fat deposit predominantly around neck.One patients had upper extremity localizations. Six patients had the complication of left ventricular diastolic function changes,glucose intolerance or diabetes mellitus, chronic hepatopathy, hyperuricemia and sleep apnea syndrome in one or more. One patients with several symptoms occur simultaneously, another one female patient was accompanied by all symptoms but chronic hepatopathy. Five male patient were alcohol abusers. Tatal neck lipentomy and abstinence from alcohol were performed on 5 patients. One patient refused treatment.During a follow-up of 3 months to 4 years, one patients was relapsed again,and no recurrence was seen in another 4 patients. All pathological results were nonencapsulated fat. Conclusion:BSL is a lipodystrophy caused by diffuse fatty tissue, symmetry deposition in the neck and shoulder subcutaneous fascia space or deep fascial space .The highest incidence favors to middle-aged man who is alcoholist. Chronic alcohol addiction and typical clinical symptoms help to diagnosis BSL. Lipectomy represents a successful procedure in treating BSL.

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