Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 23
Filter
Add filters








Year range
1.
Article in Chinese | WPRIM | ID: wpr-905884

ABSTRACT

Rotavirus enteritis (RVE), a common infectious disease of the digestive system, is commonly found in infants and young children. Its incidence is high, posing great threats to human health. At present, no specific drugs are available in western medicine and the symptomatic treatments like fluid infusion, anti-diarrhea, correction of electrolyte disorder, protection of gastrointestinal mucosa, and regulation of intestinal microecology have been employed. In addition to the poor therapeutic efficacy, they do not perform well in preventing the onset of RVE and shortening the course of disease. Traditional Chinese medicine (TCM) exerts the therapeutic effect against RVE via multiple targets, without inducing obvious adverse reactions and the cost is low. The specific mechanism of action has yet to be fully explained. Although there are some studies exploring the role of Chinese medicinal monomers in the pathogenesis of RVE, the types involved are still not enough. The effects of Chinese medicinal monomers on autophagy-related pathways fail to be uncovered, which is attributed to the lack of large-scale experimental data and clinical evidence. There are also many problems that cannot be ignored in the related research of Chinese medicinal compounds, taking the superficial exploration and limited scope for instance. Autophagy is a highly conserved biological phenomenon that involves a variety of signaling pathways. Its dysfunction is related to multiple pathological processes. Studies have shown that autophagy plays an important role in the pathogenesis of RVE, especially in the early stage of viral infection. Autophagy induces intestinal mucosal barrier damage, intestinal nerve dysfunction, and immune abnormality, resulting in the occurrence and development of RVE. In recent years, a large number of experimental studies have confirmed that TCM fights against circulatory, respiratory, digestive, and immune system diseases as well as tumors by intervening in autophagy. At the same time, a handful of studies have suggested that Chinese medicinal monomers and compounds regulate autophagy and interfere with viral replication by affecting related signaling pathways, thus playing a positive role in reversing the progression of RVE. However, at present, there are few studies on the regulation of autophagy by TCM in the treatment of RVE, and no systematic elaboration is available. This review aimed to summarize the role of autophagy in the pathogenesis of RVE and its intervention with TCM, in order to provide more theoretical and clinical evidence for the treatment of RVE with TCM.

2.
Article in Chinese | WPRIM | ID: wpr-775362

ABSTRACT

Based on the systematic summary of the results of the fourth general survey of traditional Chinese medicine resources, the cultivation of large varieties of Chinese material medica and the latest research on health industrial development, the novel concepts and scientific connotations of generalized science of Chinese material medica are put forward, and the basic ideas and methods of a new Chinese medicine academic system, the cultivation system of large varieties of Chinese medicinal materials and the application system of the large health industry are constructed. This kind of generalized science of Chinese material medica, rooted in the traditional Chinese culture and the theory of "preventive treatment of disease", can avoid the narrow prospect induced by the increasing specialization and refinement of knowledge of science of Chinese material medica. It will play an important role in the modernization, industrialization, internationalization of traditional Chinese medicine.


Subject(s)
Drug Industry , Humans , Materia Medica , Therapeutic Uses , Medicine, Chinese Traditional , Research
3.
Tianjin Medical Journal ; (12): 126-131, 2018.
Article in Chinese | WPRIM | ID: wpr-697989

ABSTRACT

Objective To observe the effect of lentiviral vector-mediated basic fibroblast growth factor (bFGF) gene transfection on the biological characteristics of rabbit bone marrow stromal cells(BMSCs)under in vitro culture conditions. Methods BMSCs were obtained by density gradient centrifugation and adherence screening. The bFGF gene was transfected into BMSCs by lentiviral vector and divided into bFGF transfection group,empty virus group and untransfected group according to the transfection conditions.After transfection,the morphology,expressions of bFGF mRNA and protein, cell proliferation,cell cycle and alkaline phosphatase(ALP)activity were observed in three groups of cells. Results High density BMSCs were successfully obtained by density gradient centrifugation and adherence screening.After transfection of BMSCs with bFGF gene, the cell morphology showed no significant changes, while the expressions of bFGF mRNA and protein were significantly increased, the cell proliferation curve shifted upward, the proportion of proliferating cells increased,and the activity of ALP was significantly enhanced.There were significant differences between three groups(P<0.05).Conclusion The rabbit bFGF gene is successfully introduced into the BMSCs cultured in vitro by lentiviral vector, and the target gene is stably expressed.The expression of bFGF can promote the proliferation and osteogenic differentiation of BMSCs.

4.
Article in Chinese | WPRIM | ID: wpr-792584

ABSTRACT

Objective To establish a rapid and accurate detecting method for sulfite in food residues. Methods Applying an fluorine ionsalkali - stable liquid to extract ultrasonically. After removing the suspended substances by high - speed centrifugation,we used online dialysis - ion chromatography to determine sulfite. Chromatographic conditionswere set as follows:Metrosep A Supp 5 - 150 / 4. 0 anion analysis column,using 3. 2 mmol / L sodium carbonate - 1. 0 mmol / L sodium bicarbonate 5% acetone as the eluent,with 0. 70 mL / min flow rate,20 μL injection volume and a conductivity detector. Results Sulfites( calculated as sulfur dioxide)manifested a good linear relationship when its concentration ranged from 0. 50 μg / mL to 50. 00 μg / mL,r = 0. 999 8. The minimum detection limit was 0. 54 mg / kg. The spiked concentrations were 10. 0 mg / kg,100. 0 mg / kg,500. 0 mg / kg,900. 0 mg / kg to samples. Relative standard deviation(n = 6,% )was 5. 04,1. 20,0. 56,0. 98,with the recovery rate between 85. 4% - 104. 4% . Conclusion This method is simple,rapid,with high sensitivity and accuracy of measurement,and which is suitable for the detection of sulfites content in food matrix.

5.
Article in Chinese | WPRIM | ID: wpr-695845

ABSTRACT

Objective To investigate the effect of electroacupuncture on renal CaM-eNOS-NO signal pathway in Dahl salt-sensitive rats with prehypertension.Methods Thirty 7-week-old male Dahl salt-sensitive rats (DS) were allocated,using a random number table,to model,acupuncture and non-meridian-acupoint groups.Ten 7-week-old male Dahl salt-resistant rats (DR) were used as a blank group.The rats were fed in a SPF animal experiment center.An 8% NaC1 high salt diet was provided after 1-week adaptive feeding.During the period,rat blood pressure was monitored using an intelligent noninvasive sphygmomanometer.A rat model of prehypertension was successfully made if rat blood pressure rose to 120~ 139/80~89 mmHg after 12 days.At that time,a high salt diet was stopped and a normal diet was provided.The blank and model groups received 15-minute regular fixation and no treatment.The acupuncture group received electroacupuncture at bilateral Quchi and Zusanli.The non-meridianacupoint group received electroacupuncture at fixed control points selected between 10 and 15 mm above bilateral iliac crests 20 mm lateral to the midline.Treatment was given once daily,6 times a week,for five consecutive weeks.The left rat kidney was taken.CaM and eNOS mRNA expressions were determined by quantitative fluorescence PCR.eNOS was quantified and located by western blot and immunohistochemical method.NO content was measured by ELISA.Results As compared with the blank group,CaM and eNOS mRNA expressions,eNOS protein content and NO content decreased significantly in the model group (all P<0.05).As compared with the model group,CaM and eNOS mRNA expressions,eNOS protein content and NO content increased significantly in the acupuncture group (all P<0.05).As compared with the model group,CaM expression increased significantly (P<0.05) but eNOS mRNA expression and NO content had no statistically significant differences (P>0.05) in the non-meridian-acupoint group.Conclusion The renoprotective effect of electroacupuncture at Quchi and Zusanli may be related to upregulating renal CaM-eNOS-NO signal pathway in DS rats with prehypertension.

6.
Article in Chinese | WPRIM | ID: wpr-789297

ABSTRACT

[ Objective] To investigate the effect of community -based comprehensive intervention on improving residents'knowledge , attitude and behaviors in terms of stroke prevention . [ Methods] By means of stratified sampling , 120 resident population were randomly selected from Xietu Neighborhood of Shanghai .Demographic characteristics , prevalence of stroke , knowledge , attitudes and behaviors related to stroke prevention were investigated through baseline survey .After 3-month interventions , end stage survey was conducted to assess the effects of the interventions by measuring the changes of the related indicators as mentioned above . [ Results] With intervention , the residents'knowledge and attitude on stroke preven-tion was raised .The number of the residents was increased participating in prevention , education and coun-seling activities on stroke prevention and those who voluntarily went to hospital for early screening on stroke were increased , too. [ Conclusion] Community-based comprehensive interventions could be effective in improving residents'knowledge , attitudes and behaviors on stroke prevention .Conducting community in-tervention on stroke is necessary .

7.
Chinese Medical Journal ; (24): 4444-4447, 2013.
Article in English | WPRIM | ID: wpr-327551

ABSTRACT

<p><b>BACKGROUND</b>Subjective assessment tools such as visual analog scales (VAS) or pain scores are commonly used to evaluate the intensity of chronic cancer-induced pain. However, their value is limited in some cases. We measured changes in VAS pain scores and salivary α-amylase (sAA) concentrations in cancer patients receiving radiotherapy for bone metastases to ascertain the correlation between these measures.</p><p><b>METHODS</b>We enrolled 30 patients with bone metastases attending a single institution from June 2010 to March 2011. All patients with cancer-induced bone pain received radiation therapy (RT) at the same dose (30 Gy) and fractionation (3 Gy/fraction, 5 days/week) for palliative pain relief. We assessed heart rate (HR), systolic and diastolic blood pressures (DBP/SBP) and VAS pain scores before (d0) and after five (d5) and ten fractions (d10) of irradiation. sAA and salivary cortisol (SC) concentrations were measured using a portable analyzer and automated chemiluminescence analyzer, respectively.</p><p><b>RESULTS</b>Radiotherapy markedly decreased VAS scores from (82.93 ± 9.29) to (31.43 ± 16.73) mm (P < 0.001) and sAA concentrations from (109.40 ± 26.38) to (36.03 ± 19.40) U/ml (P <0.001). Moreover, there was a significant correlation between these two indices (P <0.01, r = 0.541). HR decreased by 6.5% after radiotherapy, but did not correlate with VAS scores (P >0.05). SC concentrations and BP did not change significantly during the study (P >0.05).</p><p><b>CONCLUSIONS</b>The significant correlation between sAA concentrations and VAS pain scores identified in these preliminary results suggests that this biomarker may be a valuable, noninvasive and sensitive index for the objective assessment of pain intensity in patients with cancer-induced bone pain.</p>


Subject(s)
Aged , Bone Neoplasms , Radiotherapy , Female , Humans , Male , Middle Aged , Pain , Radiotherapy , Pain Management , Pain Measurement , Salivary alpha-Amylases , Metabolism , Treatment Outcome
8.
Chinese Journal of Surgery ; (12): 627-630, 2013.
Article in Chinese | WPRIM | ID: wpr-301220

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the relationship between renal clear cell carcinoma and type 2 diabetes mellitus (DM).</p><p><b>METHODS</b>Two hundreds and sixty-four patients with renal clear cell carcinoma and four hundred controls who suffered from non-urinary system, non-neoplastic or non-hormone-related disorders, were enrolled from January 2008 to December 2012. The incidence of diabetes between the 2 groups and the relationship between renal clear cell carcinoma and duration of diabetes were compared, moreover, renal clear cell carcinoma patients with DM were compared with patients without DM for their clinical features, laboratory examinations and histological characteristics.</p><p><b>RESULTS</b>The comparison of renal clear cell carcinoma group and control group: the incidence of DM in the two groups were 19.7% and 12.8% respectively, and the difference was significant (χ(2) = 5.86, P < 0.05, OR = 1.68). In the renal clear cell carcinoma group, the proportion of patients with DM diagnosed within 2-4 years was 4.92%, which were significant higher than those in the control group 1.70% (χ(2) = 5.49, P < 0.05, OR = 2.91). And men with diabetes had high occurrence risk 86% of renal clear cell carcinoma (OR = 1.86, 95%CI: 1.09-3.15). The comparison of diabetes patients subgroup and non-diabetic patients subgroup in renal clear cell carcinoma group: in respect of clinical features, greatest tumor diameter in the two subgroups were (4.9 ± 2.3) cm and (4.2 ± 2.1) cm respectively, and the difference was significant (t = 1.96, P < 0.05). However, there was no significant difference in terms of age, gender and cancer location between the two subgroups (P > 0.05). In respect of laboratory examinations, serum creatinine in the two subgroups were (72 ± 20) µmol/L and (65 ± 17) µmol/L, and the difference was significant (t = 2.34, P < 0.05); serum urea nitrogen in the 2 subgroups were (7.1 ± 2.1) mmol/L and (6.0 ± 1.5) mmol/L respectively, and the difference was significant too (t = 1.47, P < 0.05). In respect of histological characteristics, the proportion of well differentiated clear cell carcinoma were 80.8% and 81.1% respectively, and the difference was significant (χ(2) = 4.23, P < 0.05). The proportion of stage II were 25.0% and 27.8% respectively and the difference was significant (χ(2) = 4.08, P < 0.05).</p><p><b>CONCLUSIONS</b>DM is closely related with renal clear cell carcinoma and DM may be a possible risk factor for the tumor. And for elderly patients with diabetes who appear waist discomfort or hematuria, a careful examination of kidney is important to make early diagnosis, give timely treatment and improve survival prognosis.</p>


Subject(s)
Adult , Aged , Aged, 80 and over , Carcinoma, Renal Cell , Case-Control Studies , Diabetes Mellitus, Type 2 , Female , Humans , Incidence , Kidney Neoplasms , Male , Middle Aged , Prognosis
9.
Article in Chinese | WPRIM | ID: wpr-324233

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the dynamic expression of Heat shock protein 70 (Hsp70) in the lungs and plasma of rats with pulmonary fibrosis induced by silicon dioxide (SiO2).</p><p><b>METHODS</b>Forty-eight Wistar rats were randomly divided into the control group exposed to normal solution and group exposed to SiO2 (50 mg/ml) with intratracheal injection. Each group was divided into four subgroups. The animals of SiO2 group and control group were sacrificed and lungs were collected on the 7th, 14th and 28th days after exposure, respectively. The left lung tissues were examined with the histopathologic HE staining. The expression and localization of Hsp70 protein in the lung tissues were examined with western blot assay and immunohistochemistry, respectively. The expression levels of Hsp70 protein in the plasma were measured by ELISA.</p><p><b>RESULTS</b>The expression of Hsp70 in lung tissues of SiO2 group increased on the 7th day and reached the peak value on the 14th day then decreased, but still was significantly higher than that of the control group, the expression of Hsp70 in plasma of SiO2 group still was significantly higher than that of the control group (P < 0.05). The maximum expression level of Hsp70 in plasma of SiO2 group on the 21st day after exposure was 0.216 ± 0.027 µg/ml.</p><p><b>CONCLUSION</b>The expression levels of Hsp70 protein in the lung tissues and plasma of the group exposed to SiO2 significantly increased, which were associated with the process of pulmonary fibrosis. It was suggested that Hsp70 protein may play an important biological role in the pulmonary fibrosis induced by SiO2.</p>


Subject(s)
Animals , HSP70 Heat-Shock Proteins , Blood , Metabolism , Lung , Metabolism , Pathology , Male , Pulmonary Fibrosis , Metabolism , Rats , Rats, Wistar , Silicon Dioxide , Toxicity
10.
Chinese Journal of Epidemiology ; (12): 832-835, 2012.
Article in Chinese | WPRIM | ID: wpr-288095

ABSTRACT

Objective To analyze the viral genetic characteristics of hantaviruses carried by Microtus maximowixzii in Yakeshi of Inner Mongolia Autonomous Region and its relationship with Hantaan virus (HTNV) and Seoul virus (SEOV) viruses as well as to identify the natural host of Khabarovsk virus (KHAV).Methods HV specific RNAs were detected by RT-PCR.Complete S and M segment were amplified from the RNA-positive samples.Phylogenetic analysis were performed to estimate the genetic characterization and the relationship with other hantaviruses.Results Fifty two Microtus maximowixzii voles were captured in Yakeshi areas.Of those voles,hanta-viral RNA was tested positive in 5 samples (9.62%).Complete S and M segments sequences were obtained from 5 and 2 lung samples,respectively.The complete S segment was consisted of 1848 to 1861 bp,and the M segment consisted of 3662 bp.These viruses were closely related to each other with 92.5%-96.4% for the S segment sequences and 88.9%-95.4% for the M segment sequences.They shared a higher identity with KHAV found previously in Yakeshi and KHAV of Russia.However,they were obviously different from the other hantavirus species.The 5 strains had the consistent secondary structure of nucleocapsid protein (NP) and glycoprotein (GP).When further comparing their secondary structures with those of HTNV and SEOV,our results indicated that there were no obvious differences in NP between KHAV and both HNTV,SEOV but with obvious difference in GP.Based on the S and M segment sequences,phylogenetic analyses revealed that these 5 strains clustered together with KHAV and formed a distinct lineage.Furthermore,all known KHAV strains could be divided into two small branches with a nucleotide divergence more than 5.3%.Conclusion Our research data revealed that KHAV was highly endemic among Microtus maximowixzii in Yakeshi area which supported the notion that Microtus maximowixzii had been the natural host of KHAV in the area.

11.
Chinese Journal of Epidemiology ; (12): 608-612, 2011.
Article in Chinese | WPRIM | ID: wpr-273130

ABSTRACT

Objective To establish a method combined morphology and molecular marker for identifying Haemaphysalis longicomis and Rhipicephalus microplus. Methods Ticks were collected from domestic animals and wild environment in epidemic area of Hubei and Henan provinces where cases of fever with thrombocytopenia syndrome were prevalent. We classified the ticks by morphology characteristics before 12S rDNA of ticks were amplified by PCR and subsequently sequenced. Phylogenetic tree was constructed by PAUP4.0. Results The ticks belonged to Haemaphysalis longicomis and Rhipicephalus microplus through observation and analysed by the morphological characteristics of the ticks. 12S rDNA was cloned and sequenced while data confirmed the morphological identification of the results. Conclusion The method based on morphology that combined with molecular marker seemed a good method for the identificaton of ticks.

12.
Article in English | WPRIM | ID: wpr-65184

ABSTRACT

OBJECTIVE: To determine the efficacy, safety and primary follow-up results of a guidewire looping technique for the treatment of infrapopliteal arteries. MATERIALS AND METHODS: From October 2006 to May 2008, an intraluminal angioplasty of the infrapopliteal arteries was attempted in 200 consecutive patients. Altogether, 417 infrapopliteal lesions, with lengths varying from 2 cm to 32 cm, were treated as part of this study, including 305 lesions in the anterior tibial arteries, 89 in the posterior tibial arteries, and 23 in the peroneal arteries. The 'U'-shaped guidewire technique was attempted in 393 lesions from 361 limbs. The tip of a hydrophilic 0.035-inch guidewire was formed into a 'U' shape with the aid of a 4-Fr catheter and collateral branch vessel to recanalize the completely occluded long segment lesions. RESULTS: A successful angioplasty with at least one artery recanalized directly to the malleolar or dorsal foot was achieved in 322 limbs (89%). The looping technique had a success rate of 90% (352 of 393 lesions). After the procedure, the rest pain was relieved in 58 of 69 patients, while 207 of 245 limbs (85%) showed improvement for intermittent claudication. Complete wound healing was noted in 21 of 54 patients, while 20 of 54 patients showed an improvement in the wound size or depth. A total of 38 major immediate procedure-related complications were noted, including retroperitoneal hematoma, distal emboli, and vessel rupture. CONCLUSION: The results of this study suggests that the guidewire looping technique is a safe and effective method for the recanalization of the occluded lesions in infrapopliteal vessels.


Subject(s)
Aged , Aged, 80 and over , Angioplasty/methods , Ankle Brachial Index , Arterial Occlusive Diseases/surgery , Female , Follow-Up Studies , Foot/blood supply , Humans , Magnetic Resonance Angiography , Male , Middle Aged , Popliteal Artery , Postoperative Complications , Treatment Outcome
13.
Article in Chinese | WPRIM | ID: wpr-329368

ABSTRACT

This paper introduces the design and implementation of a novel respiratory detecting system based on bio-impedance method. By increasing electrodes in space, the system make multi-channel respiratory signals be superpositioned and filtered (SNR); Traditional filter methods by both hardware and software are also used to further increase anti-interference ability. A low consumption and portable instrument is designed based on MSP430 Micro Controller Unit (MCU), The experiment shows a better performance in the reduction of interference noises of heartbeat and blood flow especially the motion artifact. Also the system works stably.


Subject(s)
Electric Impedance , Electrodes , Respiratory Function Tests , Methods , Software
14.
Article in Chinese | WPRIM | ID: wpr-329367

ABSTRACT

This paper analyzed the limitation of electronic sphygmomanometer based on oscillometry, and according to the characteristics of pulse signal, the author proposed a new method of the characteristic parameter detection based on wavelet transform, and improved its recognition ability on fixed-scale, via resampling rate according to the heartbeat. And the prototype test has been proved that this method is more adaptability for individuals and stability for operation.


Subject(s)
Blood Pressure Monitoring, Ambulatory , Individuality , Signal Processing, Computer-Assisted
15.
National Journal of Andrology ; (12): 449-451, 2009.
Article in Chinese | WPRIM | ID: wpr-292353

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the effect and safety of transurethral prostatectomy with the bipolar plasmakinetic technique (PKRP) in the treatment of benign prostate hyperplasia (BPH).</p><p><b>METHODS</b>A total of 712 BPH patients underwent transurethral prostatectomy with the bipolar plasmakinetic technique. The patients averaged 70.6 years of age and 52 g (range 35-102 g) in estimated prostate weight preoperatively. Comparative analyses were made on the maximum urine flow rate (Qmax), residual urine volume and scores on IPSS and QOL obtained pre- and post-operatively.</p><p><b>RESULTS</b>The operations lasted 20-120 minutes (mean 51 min), the resected tissues weighed 15-96 g (mean 46 g), and no transurethral resection syndrome (TURS) occurred. The catheters were removed 4 -5 days after surgery. The patients were followed up for 1 -52 months (mean 27.6 mo). Obvious reduction was observed in the average Qmax from 4.7 ml/s preoperatively to 19. 1 ml/s postoperatively, in the mean IPSS score from 26.6 to 5. 8, and in the mean QOL score from 5.4 to 1.7, all with significant differences (P < 0.01).</p><p><b>CONCLUSION</b>Transurethral prostatectomy with the bipolar plasmakinetic technique is a safe and effective means for the treatment of BPH.</p>


Subject(s)
Aged , Aged, 80 and over , Humans , Male , Middle Aged , Prostatic Hyperplasia , General Surgery , Transurethral Resection of Prostate , Methods , Treatment Outcome
16.
Acta Pharmaceutica Sinica ; (12): 615-619, 2009.
Article in Chinese | WPRIM | ID: wpr-278210

ABSTRACT

The polysaccharide B3-PS2 was extracted and purified from Herba Scutellariae Barbatae through chromatography of DEAE-cellulose and Sephacryl S-300 column. Average molecular weight of B3-PS2 was about 1,100 kD. It was composed of Glc, Gal and Ara in the ratio of 2.7:2.7:1.0, along with trace of Man, Rha, Fuc and Xyl. B3-PS2 inhibited complement activation on the classic pathways with CH50 value of (0.23 +/- 0.03) mg mL(-1). The targets of B3-PS2 upon the complement system were C1r, C1s, C3 and C4. These results suggested that anti-complementary activity of B3-PS2 was closed to its positive control heparin. It strongly suggested that the polysaccharide B3-PS2 from Herba Scutellariae Barbatae could be a potential candidate in treating those complement-associated diseases.


Subject(s)
Complement Activation , Complement Inactivating Agents , Pharmacology , Drugs, Chinese Herbal , Polysaccharides , Chemistry , Pharmacology , Scutellaria , Chemistry
17.
Chinese Journal of Epidemiology ; (12): 1101-1104, 2007.
Article in Chinese | WPRIM | ID: wpr-322883

ABSTRACT

<p><b>OBJECTIVE</b>In order to better understand the epidemiological features of Hantviruses in Inner Mongolia.</p><p><b>METHODS</b>Epidemiological surveillance data during the period of the past 52 years were analyzed. An epidemiological survey was carried out in the main epidemic areas in 2005.</p><p><b>RESULTS</b>A total of 8310 hemorrhagic fever with renal syndrome (HFRS) cases were reported in Inner Mongolia from 1955 to 2006, and distributed in 61 counties. HFRS cases were mainly distributed in the east part of Inner Mongolia before 1990. However, HFRS cases had occurred in the middle and western parts since 1990. Hulunbeier prefecture, from the eastern part of Inner Mongolia, had been the most severe area being hit by HERS since the first outbreak in 1955, with 7369 cases reported over the past 52 years, and accounted for 88.68% of the total cases in the whole autonomous region. Although no HFRS cases had been reported before 1999 in Bayannaoer which located in the western part of Inner Mongolia, a total of 95 cases were reported in 2005. Hantavirus antigens had been detected in 11 species of rodents so far,including Apodemus agrarius, Rattus norvegicus, Mus Musculus, Cricetulus barabensis, meriones meridianus, Microtus maximowiczii , Clethrionomys rutilus, Apodemus peninsulae, Phodopus roborvskii, Dipus sagitta and Allactaga sibirica.</p><p><b>CONCLUSION</b>Results suggested that the epidemics might remain at a relatively high level in the years to come in Inner Mongolia. Furthermore, there might be other types of Hantaviruses in addition to the already identified Seoul viral type in this area.</p>


Subject(s)
Animals , China , Epidemiology , Disease Outbreaks , Hantavirus , Hemorrhagic Fever with Renal Syndrome , Epidemiology , Humans , Molecular Epidemiology , Rodent Diseases , Epidemiology , Virology , Rodentia , Virology , Zoonoses , Epidemiology , Virology
18.
Chinese Journal of Epidemiology ; (12): 157-159, 2007.
Article in Chinese | WPRIM | ID: wpr-232330

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the prevalence of Anaplasma phagocytophilum in rodents from forest areas in northeastern China.</p><p><b>METHODS</b>PCR amplification, followed by sequence analysis was carried out. The sequences of 16S rRNA and gltA gene fragment amplified from rodent specimens were compared with corresponding part of the sequences deposited in GenBank.</p><p><b>RESULTS</b>A total number of 276 rodents were tested, including 102 in Jilin province, 61 in Helongjiang province and 113 in Inner Mongolia autonomous region. The positive rates were 8.82%, 1.64% and 0.00%, respectively. The infection rate in rodents infected by ticks was 11.30 times higher than that in rodents without ticks (P = 0.002). The S. A. phagocytophilum 16S rRNA sequences from rodents in Jilin and Heilongjiang were identical and differed in 3-5 bases compared with the corresponding parts of A. phagocytophilum from America, Sweden and Japan. Compared with the sequences registered in GenBank, the nucleotide sequence of gltA varied from 87%-97% and its deduced amino acid sequence changed from 84%-99%.</p><p><b>CONCLUSION</b>A. phagocytophilum infection was presented in rodents from Jilin and Heilongjiang province.</p>


Subject(s)
Amino Acid Sequence , Anaplasma phagocytophilum , Genetics , Animals , Bacterial Proteins , Base Sequence , China , Ehrlichiosis , RNA, Ribosomal, 16S , Rodentia , Microbiology , Ticks , Trees
19.
Chinese Journal of Epidemiology ; (12): 681-684, 2006.
Article in Chinese | WPRIM | ID: wpr-233895

ABSTRACT

<p><b>OBJECTIVE</b>To detect and study the types of Borrelia burgdorferi sensu lato in ticks and rodents from Da Xing-An Mountains Forest areas of China.</p><p><b>METHODS</b>Nested PCR was performed to amplify 5S-23S rRNA intergenic spacer of B. burgdorferi. Positive products were analysed by restriction fragment length polymorphism (RFLP) and single strand conformation polymorphism (SSCP), specimens showing unique RFLP profile were sequenced and analysed.</p><p><b>RESULTS</b>1336 Ixodes persulcatus, 144 Dermacento silvarum, 144 Haemaphysalis concinna and 145 rodents of 9 species were collected from 16 sections of Da Xing-An Mountains Forest areas of China. Specific fragments were amplified from 293 I. persulcatus and 6 D. silvarum and 5 rodents of 4 species. B. burgdorferi was not detected in H. concinna. Among the positively tested I. persulcatus, 209 contained B. garinii genospecies and 45 contained B.afzelii genospecies based on RFLP. Moreover, B.garinii genospecies consisted of B. garinii 20047 and B. garinii NT29. 17 adult I. persulcatus were simultaneously infected with B. garinii 20047 and B. garinii NT29. Nine adult I. persulcatus were simultaneously infected with B. garinii 20047 and B. afzelii. Four adult I. persulcatus were simultaneously infected with B. garinii 20047 and B. garinii NT29 and B. afzelii. Two D. silvarum were infected with B. garinii 20047, 1 D. silvarum with B. garinii 20047, 2 D. silvarum with B. afzelii. 3 rodents were infected with B. garinii 20047 while 2 rodents were infected with B. garinii NT29. Mixed infection was not found in D. silvarum and rodents. In addition, nine I. persulcatus and one D. silvarum specimens showed unique RFLP pattern. Data from sequential analysis showed that they all belonged to B. garinii. PCR-SSCP profiles of 5S-23S rRNA intergenic spacer of B. burgdorferi in the positive specimens exceeded 36 types; B. garinii 20047 showed 16 types while B. garinii NT29 showing 11 types, B. afzelii showing 9 types. SSCP profiles of the specimens coinfected with multiple B. burgdorferi was relatively complex.</p><p><b>CONCLUSION</b>The infection of B. burgdorferi was found in the ticks and rodents in Da Xing-An Mountains Forests areas. The infection rate of I. persulcatus was high. B. garinii was predominant genospecies, and the population of B. burgdorferi was heterogeneous in the area. Mixed infections of different B. burgdorferi genospecies in ticks were found. I. persulcatus and Clethrionomys rufocanus were possibly served as major vector and major host for B. burgdorferi, respectively, suggesting that further study is needed to confirm the coinfection in humans and animals in this region.</p>


Subject(s)
Animals , Borrelia burgdorferi Group , Genetics , China , Epidemiology , Humans , Lyme Disease , Epidemiology , Polymorphism, Restriction Fragment Length , Polymorphism, Single-Stranded Conformational , RNA, Bacterial , Rodentia , Microbiology , Ticks , Microbiology , Trees
20.
National Journal of Andrology ; (12): 140-144, 2005.
Article in Chinese | WPRIM | ID: wpr-323410

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the effect and safety of transurethral prostatectomy with the bipolar plasma kinetic technique (PKRP) in the treatment of benign prostate hyperplasia(BPH).</p><p><b>METHODS</b>Two hundred and ninty-seven BPH patients underwent transurethral prostatectomy with the bipolar plasma kinetic technique. The preoperative estimated weight of the prostate ranged from 35 g to 102 g, averaging 52 g.</p><p><b>RESULTS</b>The operation lasted 40 approximately 65 min, averaging 51 min. The resected tissues weighed 40 approximately 80 g, averaging 46 g. During the operation no transurethral resection (TUR) syndrome occurred. The catheter was removed 4 approximately 5 days after the operation, all with fluent urination. The patients were followed up for 2 approximately 33 months. IPSS decreased from average 31.5 preoperatively to average 6.8 postoperatively (P < 0.001). Average maximum flow-rate (Q(max)) decreased from 6.3 ml/s preoperatively to 18.6 ml/ s postoperatively (P < 0.001). Preoperative average residual urine was 97 ml and reduced to average 9 ml after the operation. Temporary incontinence occurred in 4 cases, perioperative hemorrhage in 2, and urethral stricture in 1.</p><p><b>CONCLUSION</b>Transurethral prostatectomy with the bipolar plasma kinetic technique is a safe and effective means for the treatment of BPH.</p>


Subject(s)
Aged , Aged, 80 and over , Humans , Male , Middle Aged , Prostatic Hyperplasia , General Surgery , Transurethral Resection of Prostate , Methods , Treatment Outcome
SELECTION OF CITATIONS
SEARCH DETAIL