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1.
Article in Chinese | WPRIM | ID: wpr-912877

ABSTRACT

Objective: To evaluate the effectiveness of therapeutic massage (tuina) for treating knee osteoarthritis (KOA). Methods: Six English and Chinese databases, including Chinese National Knowledge Infrastructure (CNKI), Wanfang Academic Journal Full-text Database (Wanfang), Chongqing VIP Database (CQVIP), China Biology Medicine Disc (CBM), Cochrane Library and PubMed databases, were independently searched to identify appropriate randomized controlled trials (RCTs) studying therapeutic massage for KOA compared to oral non-steroidal anti-inflammatory drugs (NSAIDs) alone. The main outcome measures were total effectiveness and the Western Ontario and McMaster Universities osteoarthritis index (WOMAC) score. Results: A total of 8 RCTs were included and they were of average quality. The results showed that therapeutic massage was more effective than NSAIDs comparing total effectiveness [risk ratio (RR)=1.14, 95% confidence interval (CI) (1.07, 1.21), P<0.0001]; compared with NSAIDs, therapeutic massage produced more significant improvements in pain [mean difference (MD)=-2.06, 95%CI (-2.75, -1.36), P<0.00001], stiffness intensity [MD=-0.90, 95%CI (-1.05, -0.75), P<0.00001] and joint function [MD=-12.48, 95%CI (-13.91, -11.05), P<0.00001]. Conclusion: Therapeutic massage was more effective than oral NSAIDs in treating KOA. In relieving pain and stiffness and improving the function of knee joint, therapeutic massage was superior to NSAIDs.

2.
Article in Chinese | WPRIM | ID: wpr-912867

ABSTRACT

According to Chinese medicine, the atlantoaxial joint is a composite joint composed of tendons and bones, and the stability of the joint depends on the 'tendon-bone balance' involving tendons, ligaments, atlas and axis. Multiple causes of 'tendon off-position, joint subluxation' will lead to joint 'tendon-bone imbalance', which will evolve into atlantoaxial subluxation (AAS), endangering human health. Chinese therapeutic massage (tuina) is a very effective treatment for AAS in adults, but conventional manipulations are prone to ineffectiveness or accidents due to neglect of the causal relationship of the 'tendon-bone imbalance' and inappropriate manipulations. Compared with conventional manipulations, the rational choice of modified manipulations under the guidance of 'tendon-bone balance' theory is more effective and less risky, and more worthy of clinical promotion. From the 'tendon-bone balance' theory, we considered the shortcomings of conventional manipulations, and introduced several modified manipulations that have their own strengths in 'tendon smoothing' and 'bone setting', in order to provide new ideas for treatment of AAS in adults.

3.
Article in Chinese | WPRIM | ID: wpr-904623

ABSTRACT

Objective To investigate the changes of mitochondrial metabolic functions of macrophages following Echinococcus multilocularis infections, so as to provide insights into the pathogenesis of alveolar echinococcosis. Methods Two groups were assigned according to different treatment methods. In the culture group, mouse leukemic monocyte macrophage RAW264.7 cells were cultured with 2 000 E. multilocularis at a ratio of 500∶1, while RAW264.7 cells in the control group were given no treatment. Then, both the culture and control groups were further divided into the 24 h and 72 h subgroups. Mitochondria were stained with MitoTracker® Deep Red FM and the mean fluorescence intensity of macrophage mitochondria was measured with the Cytation 5 Cell Imaging Multi-Mode Reader. The mitochondrial DNA copy number was quantified using the quantitative real-time PCR (qPCR) assay, and the mitochondrial energy metabolism was monitored using the Seahorse XF assay. In addition, the mitochondrial reactive oxygen species and mitochondrial membrane potential were detected using flow cytometry. Results The mean fluorescence intensities of macrophage mitochondria were significantly lower in the 24 h (15 341 ± 2 532 vs. 17 823 ± 3 429; t = 6.379, P < 0.01) and 72 h (18 102 ± 3 505 vs. 21 511 ± 5 144; t = 17.680, P < 0.01) culture subgroups than in the corresponding control subgroups, and lower mitochondrial DNA copy numbers were measured in the 72 h culture subgroup than in the 72 h control group [(3.23 × 109 ± 1.78 × 107) vs. (4.39 × 109 ± 3.70 × 107); t = 8.85, P < 0.001]. The oxygen consumption rates were significantly greater in the 24 h [(241.70 ± 73.13) pmol/min vs. (69.05 ± 52.30) pmol/min; t = 7.89, P < 0.01] and 48 h culture groups [(249.50 ± 42.06) pmol/min vs. (60.28 ± 40.66) pmol/min; t = 8.64, P < 0.01] than in the corresponding control groups, and a higher extracellular acidification rate was seen in the 48 h culture group than in the 48 h control group ([ 111.6 ± 17.49) mpH/min vs. (35.05 ± 7.57) mpH/min; t = 16.90, P < 0.01]. In addition, flow cytometry detected higher mean fluorescence intensity of mitochondrial reactive oxygen species (58 264 ± 10 087 vs. 4 307 ± 97; t = 12.930, P < 0.01) and lower mitochondrial membrane potential (9.833% ± 2.285% vs. 2.667% ± 0.208%; t = 6.645, P < 0.01) in the 72 h culture group than in the control group. Conclusions E. multilocularis infection may impair mitochondrial functions and inhibit oxidative phosphorylation of macrophages, resulting in increased macrophage glycolysis. It is speculated that the alteration of macrophage metabolic states may contribute to the mechanisms underlying the development and progression of alveolar echinococcosis.

4.
International Eye Science ; (12): 302-306, 2019.
Article in Chinese | WPRIM | ID: wpr-713019

ABSTRACT

@#AIM: To investigate the prevalence of strabismus and amblyopia in Dai, Hani, Lahu and local Han nationality pupils in Xishuangbanna, Yunnan Province, and to analyze its differences and risk factors.<p>METHODS: A cross-sectional study of 7214 patients aged 6-15 years were conducted in Mengla County of Xishuangbanna, Yunnan Province. The investigation included naked eye and best corrected visual acuity, diopter, eye position, anterior segment and fundus examination. The influencing factors were analyzed by Logistic regression analysis.<p>RESULTS:(1)The case rate of strabismus(exotropia)and amblyopia(refractive amblyopia)were 2.12%(85.62%)and 0.60%(74.42%)respectively;(2)The case rate of strabismus had no difference in sex, age and nationality(<i>P</i>>0.05), however, the case rate of exotropia was different in ethnic groups: the Lahu is more likely to have exotropia than the Han nationality(<i>OR</i>: 1.86, 95% <i>CI</i>: 1.12-3.09). Immediate family with strabismus or exotropia, myopia and hyperopia were all risk factors of strabismus(<i>P</i><0.05 <i>OR</i>>1), and myopia was the risk factor of exotropia(<i>OR</i>: 2.13, 95% <i>CI</i>: 1.32-3.44). The <i>OR</i> value of exotropia increased with the diopter of myopia(<i>P</i><0.05);(3)The case rate of amblyopia was not different in sex, age and nationality(<i>P</i>>0.05). Myopia and hyperopia were risk factors of amblyopia(<i>P</i><0.05, <i>OR</i>>1).<p>CONCLUSION: There is a high risk of strabismus in the children whose immediate family has exotropia of Xishuangbanna ethnic minority, and there is a certain ethnic difference in strabismus; Both myopia and hyperopia are the main risk factors of strabismus and amblyopia. The disease risk of strabismus and exotropia increases with the increase of refractive diopter; Low local amblyopia was associated with a lower incidence of ametropia.

5.
Journal of Medical Postgraduates ; (12): 300-303, 2018.
Article in Chinese | WPRIM | ID: wpr-700822

ABSTRACT

Objective Epicardial fat volume(EFV)is the risk factor of coronary heart disease(CHD)and positive remod-el of coronary artery.This study aimed to investigate the association of the unit surface area Epicardial fat volume(Epicardial fat volume indexed to the body surface area,EFVi)with the risk of coronary heart disease(CHD)and vascular remodeling. Methods A total of 208 consecutive patients with suspected CHD underwent coronary computed tomography angiography(CTA)were selected between December 2016 and December 2017 in cardiology department, Nanjing General Hospital of Nanjing Military Area Command. They were divided into CHD group(n=135)and non-CHD group(n=73). 135 CHD group patients were divided into 2 subgroups according to the type of vascular Remodeling-positive remodeling group[vascu-lar remodeling index(RI)>1.05,n=56]and non-positive remode-ling group[vascular remodeling index(RI)<1.05,n=79]. Clinical data was collected and peripheral blood lipid was detected in the same period;EFV and RI were determined by coronary CTA and software on a workstation[Syngo MMWP(VE32B)];.EFVi was calculated and the association with plate property and vascular remodelingwas explored;To investigate the risk factor of vascular remodeling Results The EFVi and EFV[(128.91±52.22)cm3,(74.12±28.77) cm3/m3]of CHD group were obviously higher than that of non-CHD group[(76.14±30.40)cm3,(45.41±16.97)cm3/m3](P<0.01). Logistic regression analysis showed that EFVi(OR=1.06,95% CI:1.02-1.10),LDL-C(OR=2.56,95% CI:1.06-6.12),EFV (OR=1.05,95% CI:1.02-1.07)were the risk factors of CHD.Compared with the positive remodeling group[(128.91±52.22)cm3, (74.12±28.77)cm3/m3]of CHD group were obviously higher than that of non-CHD group[(17.25±6.43),(38.15±10.56)μm3], the fiber volume[(23.66±9.05)μm3]and calcification volume[(182.75±46.51)μm3]were significantly higher in non-positive re-modeling group. While EFVi[(68.31±25.16)cm3/m3]was significantly lower than the positive remodeling group[(92.15±31.04) cm3/m3](P<0.05). Hypertension(OR=2.78,95% CI:1.12-6.88),TG(OR=1.96,95% CI:1.04-3.70),LDL-C(OR=3.91, 95% CI:2.58-7.51)and EFVi(OR=1.02,95% CI:1.01-1.31)were high risk factors of vascular remodeling(P<0.05). Conclusion EFVi can be used as an important predictor of coronary heart disease risk and coronary vascular positive remodeling.

6.
Article in Chinese | WPRIM | ID: wpr-698655

ABSTRACT

BACKGROUND: Studies have confirmed that monosialotetrahexosyl ganglioside can induce human umbilical cord mesenchymal stem cells to differentiate into neuron-like cells, but little is reported on its optimal concentration. OBJECTIVE: To explore the optimal concentration of monosialotetrahexosyl ganglioside that induces human umbilical cord mesenchymal stem cells to differentiate into neuron-like cells in vitro. METHODS: Human umbilical cord mesenchymal stem cells were isolated by using collagenase digestion method, and after expansion, passage 3 cells were randomly allocated into five groups. When 70%-80% of cells were confluent, 50, 100, 150 and 200 mg/L monosialotetrahexosyl ganglioside induction solutions were added in corresponding experimental groups, while cells in the blank control group were cultured in the same volume of L-DMEM medium. Cell morphology was observed under inverted phase contrast microscope. Expression levels of microtubule-associated protein 2, neurofilament protein and glial fibrillary acidic protein were measured by using immunohistochemistry at 6 hours after induction. RESULTS AND CONCLUSION: Human umbilical cord mesenchymal stem cells were isolated successfully and sub-cultured stably. These cells could express surface markers of mesenchymal stem cells. Monosialotetrahexosyl ganglioside at the optimal concentration of 150 mg/L was confirmed to induce the neuron-like differentiation of human umbilical cord mesenchymal stem cells, and differentiated cells could express microtubule-associated protein 2 and neurofilament protein as neuron-specific markers.

7.
National Journal of Andrology ; (12): 1007-1013, 2017.
Article in Chinese | WPRIM | ID: wpr-812841

ABSTRACT

Objective@#To compare the clinical effect of a novel disposable circumcision device Ring with that of conventional circumcision in the treatment of redundant prepuce and phimosis.@*METHODS@#Totally, 750 patients with redundant prepuce or phimosis underwent Ring circumcision (group A, n = 450) or conventional circumcision (group B, n = 300). We recorded the operation time, intraoperative blood loss, Visual Analogue Scale (VAS) intraoperative pain scores, postoperative complications, wound healing time, and patients' satisfaction with postoperative penile appearance, followed by comparison of the collected data between the two groups of patients.@*RESULTS@#All the operations were successfully completed. Group A, as compared with B, showed significantly shorter operation time ([3.78 ± 0.42] vs [26.24 ± 3.99] min, P 0.05) or the postoperative incidence rate of edema (0.89% [4/450] vs 2.33% [7/300], P >0.05). There were no significant postoperative infections or delayed incision healing except for 1 case of wound dehiscence in each group.@*CONCLUSIONS@#Ring circumcision, with its advantages of shorter operation time, less blood loss and pain, higher safety, and better postoperative penile appearance, is easily accepted by the patients and deserves wide clinical application.


Subject(s)
Blood Loss, Surgical , Circumcision, Male , Disposable Equipment , Humans , Incidence , Male , Operative Time , Pain Measurement , Patient Satisfaction , Penis , Congenital Abnormalities , General Surgery , Phimosis , General Surgery , Postoperative Complications , Postoperative Hemorrhage , Postoperative Period , Wound Healing
8.
Acta Physiologica Sinica ; (6): 661-668, 2016.
Article in English | WPRIM | ID: wpr-331618

ABSTRACT

The present study was aimed to investigate the role of necroptosis in the pathogenesis of acute respiratory distress syndrome (ARDS). The rat model of ARDS was induced by intravenous injection of oleic acid (OA), and observed for 4 h. The lung injury was evaluated by arterial blood gas, lung wet-dry weight ratio (W/D) and histological analyses. Simultaneously, bronchoalveolar lavage fluid (BALF) was collected for total and differential cell analysis and total protein determination. Tumor necrosis factor alpha (TNF-α) level in BALF was determined with a rat TNF-α ELISA kit. Expressions of receptor interacting protein kinase 1 (RIPK1), RIPK3 and mixed lineage kinase domain-like protein (MLKL) in lung tissue were determined by Western blot and immunohistochemical staining. The interaction between RIPK1 and RIPK3 was explored by immunoprecipitation. The results showed that, compared with those in control group, total white blood cells count (WBC), polymorphonuclear percentage (PMN%), total protein concentration, TNF-α level in BALF, W/D, and the alveolar-arterial oxygen tension difference (P(A-a)O) in OA group were significantly increased at 4 h after OA injection. Western blot and immunostaining further showed remarkably increased expressions of RIPK1, RIPK3 and MLKL in lung tissue from OA group. Additionally, immunoprecipitation results indicated an enforced interaction between RIPK1 and RIPK3 in OA group. Collectively, the TNF-α level in BALF and the RIPK1-RIPK3-MLKL signaling pathway in lung tissue were found to be upregulated and activated with the process of ARDS. These findings implicate that RIPK1/RIPK3-mediated necroptosis plays a possible role in the pathogenesis of ARDS, which may provide a new idea to develop novel drugs for the therapy of ARDS.


Subject(s)
Acute Disease , Animals , Bronchoalveolar Lavage Fluid , Disease Models, Animal , Lung Diseases , Necrosis , Oleic Acid , Rats , Receptor-Interacting Protein Serine-Threonine Kinases , Respiration Disorders , Signal Transduction , Tumor Necrosis Factor-alpha
9.
Acta Physiologica Sinica ; (6): 767-774, 2016.
Article in Chinese | WPRIM | ID: wpr-331605

ABSTRACT

In this study, we investigated the protective effect of the antioxidant N-acetyl-L-cysteine (NAC) on the lung inflammation caused by ozone (O) exposure in mice. Thirty-two C57BL/6 mice were randomly divided into control group, Ogroup, O+NAC group and NAC group. Mice were exposed to O(1.0 ppm) or fresh air for 3 h on the day 1, day 3 and day 5, respectively. NAC (100 mg/kg) was intraperitoneally applied to the mice 1 h before each exposure. At 24 h after the 3-time exposure, the alveolar wall structure was severely damaged and the infiltrated inflammatory cells were apparent perivascularly and peribronchiolarly. Significant increases in the total white blood cell count, macrophage, lymphocyte and neutrophil counts, as well as total protein concentration were observed in the bronchoalveolar lavage fluid (BALF) (P < 0.05). The IL-6, IL-8 (P < 0.01) and MDA levels (P < 0.05) in the lung homogenates were elevated coherently. Administration of NAC could attenuate the alveolar wall structure damage induced by Oexposure and reduce the amount of infiltrated inflammatory cells, total and differential leukocyte counts (P < 0.05), as well as the IL-6, IL-8 (P < 0.01) and MDA release (P < 0.05). Western blotting results showed that the Oexposure up-regulated the p38 MAPK and NF-κB p65 protein expression in the lung tissue of mice (P < 0.05), which could be alleviated by NAC (P < 0.05). These results indicated that NAC could protect against O-induced pulmonary inflammation in mice. The beneficial effect of NAC might be related with the p38 MAPK and NF-κB p65 signal pathway.


Subject(s)
Acetylcysteine , Animals , Antioxidants , Bronchoalveolar Lavage Fluid , Interleukin-6 , Lung , Mice , Mice, Inbred C57BL , NF-kappa B , Neutrophils , Ozone , Pneumonia
10.
International Eye Science ; (12): 1882-1883, 2014.
Article in Chinese | WPRIM | ID: wpr-642023

ABSTRACT

AIM: To evaluate the effect of hexuemingmu tablet on retinal vein occlusion ( ROV) . METHODS: Totally, 108 patients of 112 eyes were divided into two groups randomly. One group including 55 patients ( 55 eyes ) were treated by hexuemingmu tablet, the other group including 53 patients ( 57 eyes ) were treated by danhong injection. The visual acuity, fundus and FFA were evaluated before and after treatment. RESULTS: The total effective rate in the group treated by hexuemingmu tablet was 98%, and that in control group was 82%. The hemorrhage was absorbed much more quickly than control group. Two group were different from each other in statistics (PCONCLUSION: Hexuemingmu tablet can accelerate hemorrhage absorbing and improve visual acuity in RVO treatment.

11.
Article in Chinese | WPRIM | ID: wpr-299835

ABSTRACT

Naringenin (1) was transformed to three metabolites (2-4) by Mucor sp. Based on LCMS(n)-IT-TOF and NMR spectroscopic data, 2-4 were identified as naringenin-7-O-sulphate, naringenin-4'-O-sulphate, and naringenin-5-O-sulphate, respectively. These results might provide hints to the mammalian/human metabolism of naringenin.


Subject(s)
Biotransformation , Drugs, Chinese Herbal , Chemistry , Metabolism , Flavanones , Chemistry , Metabolism , Magnetic Resonance Spectroscopy , Molecular Structure , Mucor , Metabolism , Sulfates , Metabolism
12.
Acta Pharmaceutica Sinica ; (12): 161-169, 2013.
Article in Chinese | WPRIM | ID: wpr-235687

ABSTRACT

The prenylation of aromatic compounds plays an important role in the natural product research because it not only gives rise to an astounding diversity of primary and secondary metabolites in plants, fungi and bacteria but also enhances the bioactivities and bioavailabilities of these compounds. However, further investigation of prenylated aromatic compounds is frequently hindered due to their low content in nature and difficulties in chemical synthesis. Cloning aromatic prenyltransferase genes followed by heterologous expression would be attractive tools for the chemoenzymatic synthesis of bioactive molecules. This review summarizes the classifications, structural investigations, enzymatic catalysis and other progress in aromatic prenyltransferases originated from microorganisms.


Subject(s)
Bacteria , Dimethylallyltranstransferase , Chemistry , Classification , Fungi , Molecular Structure , Substrate Specificity , Synthetic Biology
13.
Article in Chinese | WPRIM | ID: wpr-355793

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of latent and active pulmonary tuberculosis(TB) on expression of miR-29 family and target gene IFN-γ in CD4(+)T cells.</p><p><b>METHODS</b>Subjects from two hospitals of Weifang were enrolled from March 2012 to December 2012 and divided into three groups: active TB group(30 cases), latent tuberculosis infection(LTBI) group(25 cases) and healthy control group(30 cases). CD4(+) T cells in blood were collected from the three groups.Levels of miR-29a, miR-29b and miR-29c were measured by nucleic acid hybridization and RT-qPCR.Expression of IFN-γ was analyzed by RT-qPCR. Target genes of miR-29 family were predicted with both TargetScan and PicTar.GO annotation and pathway overrepresentation were further analyzed with David database and Cytoscape.</p><p><b>RESULTS</b>Levels of miR-29a, miR-29b and miR-29c showed significant differences among the three groups(P < 0.05): levels of miR-29b and miR-29c in the active TB group(561.63 ± 65.36, 281.85 ± 42.78) were higher than the healthy controls(260.74 ± 38.69, 128.21 ± 19.98), but lower than the LTBI group(2030.29 ± 321.68, 620.93 ± 79.14); expression of miR-29a in the healthy control group(913.95 ± 104.73) were higher than the active TB group(323.37 ± 54.38), but lower than the LTBI group(4782.13 ± 567.81).Level of IFN-γ showed significant differences among the three groups(P < 0.05): level of IFN-γ in the LTBI group(0.45 ± 0.09) were lower than the healthy controls(1.00), but higher than the active TB group(0.11 ± 0.03). The target genes of miR-29 family mainly existed in molecular function such as extracellular matrix structural constituent and transcription regulator activity.In KEGG pathway, the gene set mostly existed in signaling pathway such as Focal adhesion,ECM-receptor interaction and mTOR signaling pathway.</p><p><b>CONCLUSION</b>The expression of miR-29 family was increased and target gene IFN-γ in CD4(+) T cells was decreased by latent and active pulmonary TB, which might play important role in alteration of signal pathway.</p>


Subject(s)
Adolescent , Adult , CD4-Positive T-Lymphocytes , Metabolism , Case-Control Studies , Computational Biology , Female , Gene Expression , Humans , Interferon-gamma , Genetics , Metabolism , Latent Tuberculosis , Genetics , Allergy and Immunology , Male , MicroRNAs , Genetics , Middle Aged , Young Adult
14.
Article in Chinese | WPRIM | ID: wpr-315042

ABSTRACT

A total of 24 biologically pure entophytic fungal strains were isolated from stems, leaves, and seed coats of Xylocarpus plants by repeated purification, and identified with Internal Transcribed Spacer (ITS) rDNA molecular method, which belonging to 14 genera, 11 families, 9 orders and 3 classes. There were differences in genus and species levels among three plant materials from different habitats and species, and it was found that the strains of Phomopsis and Colletotrichum existed in all three plant materials. In vitro assay of antitumor activity by MTT method revealed that the EtOAc extracts of 15 strains exhibited potent antitumor activity. These results suggest that it is of value for further investigation on the above fungal strains.


Subject(s)
Antineoplastic Agents , Pharmacology , Biodiversity , Cell Line, Tumor , Endophytes , Chemistry , Classification , Genetics , Fungi , Chemistry , Classification , Genetics , HCT116 Cells , Humans , Meliaceae , Microbiology , Phylogeny
15.
Article in Chinese | WPRIM | ID: wpr-332754

ABSTRACT

This study was aimed to investigate one case with rare type B(A) in ABO blood group by using serological and molecular biological methods, and analyze the cause of inconsistency resulting from multiple detections. The serological method was used to identify the serum type of ABO blood group, at the same time the PCR sequencing method was used to detect the genotypes. The results indicated that the group typing and reverse typing for the blood donor were inconsistent, the group typing was AB, the reverse typing was B. The ABO genotype was B(A) 04 /001. This genotype was involved in nt640A > G point mutation which caused valine replacing methionine at 214. It is concluded that the sample inconsistent between the group typing and reverse typing could be typed by molecular biological method, and the molecular basis of weak expression of ABO blood group is elucidated too.


Subject(s)
ABO Blood-Group System , Genetics , Blood Donors , Blood Grouping and Crossmatching , Methods , Genotype , Humans , Serologic Tests
16.
Chinese Journal of Epidemiology ; (12): 879-882, 2012.
Article in Chinese | WPRIM | ID: wpr-289621

ABSTRACT

Objective To evaluate the long-term effectiveness of the first set 8 methadone maintenance treatment (MMT) clinics in China. Methods Repeated cross-sectional surveys were conducted on the first month after the enrollment and 5 years later,among drug users who received MMT,using a standard questionnaire.Data on demographic characteristics,HIV-related high-risk bchaviors,criminal records associatcd with drug use and related family/social functions were collected and analyzed.Results There were 252 and 195 participants being interviewed at the baseline and the 5-year surveys,respectively,.Of them,66 participants were involved in both surveys.There was no significant differences on factors as ethnicity,level of education,working status,marital status and living status (P>0.05) between the baselinc and the 5-year surveys.Compared with data from the baseline survey,participants' behavior on drug abuse (100.0% vs.24.1%,P<0.001 ),needle sharing behavior ( 19.4% vs.0.0%,P < 0.001 ),and exchanging sex for drugs ( 34.5 % vs.0.0%,P<0.001 ) had significant decreases at the 5-year survey.Rates on condom use ( 10.6% vs.25.0%,P=0.004),and having jobs (27.8% vs.47.7%,P<0.001 ) had been improved significantly,while self-reported criminal cases related to drug use (15.1% vs.1.5%,P<0.001) and the contacts with drug users (88.9% vs.31.3%,P<0.001) had been significantly reduced after joining the MMT program.Conclusion MMT could play an active role in reducing the HIV-related high-risk behaviors,criminal cases associated with drug abuse as well as enhancing the family and social functions of the MMT patients.Providing high quality service to the MMT clinic and trying to keep the drug users stick to the program remain difficult.It was also important to improve the training programs for staff working at the MMT clinics.

17.
Article in Chinese | WPRIM | ID: wpr-326194

ABSTRACT

<p><b>OBJECTIVE</b>To describe the characteristics and factors associated with long-term retention for methadone maintenance treatment (MMT) patients.</p><p><b>METHODS</b>This study was conducted in eight MMT clinics located in Sichuan, Yunnan, Guangxi, Guizhou and Zhejiang provinces. Five hundred and thirty-nine MMT patients who enrolled in MMT clinics in 2004 and retained in treatment by June 2010 were selected as study subjects. Chi-square tests were used to compare the demographics and drug abuse history at enrollment and treatment characteristics during the follow-up period between continuous treatment patients and discontinuous treatment patients.</p><p><b>RESULTS</b>Of the 539 patients, 110 (20.4%) were continuous treatment patients whereas 429 (79.6%) were discontinuous treatment patients. Of these 429 discontinuous treatment patients, 84.1% (361/429) had 2-4 treatment episodes whereas 15.9% (68/429) had 5 or more episodes during follow-up period. When continuous treatment patients were compared with discontinuous treatment patients, living with family members or friends (88.2% (97/110), 78.5% (337/429)), age of first drug use under 25 (61.8% (68/110), 71.3% (306/429)), low urine morphine positive test results (67.3% (74/110), 38.2% (164/429)) and living within 5 kilometers of the MMT clinic (72.7% (80/110), 61.3% (263/429)) were positively associated with higher possibility of continuous treat retention (P < 0.05). Demographics and drug abuse characteristics at enrollment, including gender, age, employment status, family relationship, injection, needle sharing, criminal behavior, contacts with drug users, MMT daily dosage and family members receiving MMT were not significantly associated with treatment retention (P > 0.05).</p><p><b>CONCLUSION</b>Illicit drug use during the treatment and longer distance travelling to MMT clinic might have negative impact on patients' continuous treatment retention. Mobile MMT vehicles and expanded MMT service sites could be introduced to improve compliance of treatment retention of MMT patients.</p>


Subject(s)
Adult , Female , Humans , Longitudinal Studies , Male , Methadone , Therapeutic Uses , Patient Compliance , Substance-Related Disorders , Drug Therapy , Treatment Outcome
18.
Chinese Journal of Surgery ; (12): 139-143, 2012.
Article in Chinese | WPRIM | ID: wpr-257538

ABSTRACT

<p><b>OBJECTIVE</b>To discuss the advantages and disadvantages of transcallosal-interforniceal approach for resection of the third ventricle and the pineal region tumors.</p><p><b>METHODS</b>The clinical data of 24 cases from July 2008 to March 2011 were retrospectively analyzed. All 24 patients operated by transcallosal-interforniceal approach, among them, there were 14 males and 10 females, with a average age of 32 years ranged from 17 to 65 years and with medical history from 1 month to 10 years. Issues of managements were analyzed and discussed, including reasonable incision design, the managements of draining vein, the site and the length of the incision of the corpus callosum, tumor exposure in increased intracranial pressure, prevention of complications, skills of surgery, treatments of obstructive hydrocephalus, and postoperative managements.</p><p><b>RESULTS</b>In the 24 cases, there were 5 cases of pineal parenchymal tumors, 4 cases of germinoma, 3 cases of astrocytoma, 2 cases of hypothalamus hamartomas, 2 cases of ependymoma, 2 cases of mixed germ cell tumour, 2 cases of malignant lymphomas, 1 case of pineoblastoma, 1 case of dermoid cyst, 1 case of chordoid glioma and 1 case of craniopharyngioma. After surgeries, total removal achieved in 9 cases, and subtotal removal in 10 cases and partial removal in 5 cases. Operative mortality was 0. Combined third ventriculostomy were performed in 13 cases. Postoperative complications occurred in 5 cases, including frontoparietal epidural hematoma in 1 case; postoperative short-term memory loss in 3 cases, postoperative memory loss within 1 month in 2 cases and within 3 months in 1 case; frontoparietal subdural effusion in 1 case and the effusion disappeared without any treatment. Ventriculoperitoneal shunt was performed in 1 case.</p><p><b>CONCLUSIONS</b>The transcallosal-interforniceal approach is ideal for the removal of tumors in third ventricle as well as majority tumor in posterior of third ventricle in a skillful hand. Tumor resection combined with third ventriculostomy is the significant advantages in the approach.</p>


Subject(s)
Adolescent , Adult , Aged , Brain Neoplasms , General Surgery , Female , Follow-Up Studies , Humans , Male , Microsurgery , Middle Aged , Retrospective Studies , Third Ventricle , Young Adult
19.
Article in Chinese | WPRIM | ID: wpr-351671

ABSTRACT

<p><b>OBJECTIVE</b>To study the effects of Tuina on muscular tension of flexor and extensor in patients with knee osteoarthritis (KOA).</p><p><b>METHODS</b>From June 2009 to March 2010, 20 patients with KOA were observed, including 7 males and 13 females, the age ranged from 42 to 72 years with an average of 61 years. Eight patients were ailed with single knee, 12 patients were ailed with double knee. The maneuver of Tuina were Gun maneuvers (120 to 140 times per minute), An maneuvers, Na maneuvers and Ca maneuvers. The patients were treated 3 times every week,and 10 times were 1 course of treatment. The muscular tension of flexor and extensor were measured before and after treatment, and the press force of the point reached largest of L 500 g when the press was 500 g. The therapeutic effects were assessed by WOMAC (including pain, stiffness, functional activity and overall score) scale before and after treatment.</p><p><b>RESULTS</b>There was significant increase in the ill knee on L 500 g (P<0.01). There was significant decrease after treatment in WOMAC (P<0.01). There was significant positive correlation between the improvement rate of extensor and the improvement rate of therapeutic effects (P<0.01, r=0.764). There was significant positive correlation between the improvement rate of flexor and the improvement rate of therapeutic effects (P<0.01, r2=0.533).</p><p><b>CONCLUSION</b>Tuina can improve muscular tension of flexor and extensor in patients with knee osteoarthritis, thus can improve the therapeutic effects.</p>


Subject(s)
Adult , Aged , Female , Humans , Male , Manipulation, Orthopedic , Middle Aged , Muscle Tonus , Muscle, Skeletal , Osteoarthritis, Knee , Therapeutics
20.
Acta Pharmaceutica Sinica ; (12): 75-80, 2011.
Article in Chinese | WPRIM | ID: wpr-353327

ABSTRACT

This article is report the study of the pharmacokinetics and metabolic disposition of exogenous phosphocreatine (PCr) in rats by means of an ion-pair HPLC-UV assay. PCr and its metabolite creatine (Cr) and related-ATP in rat plasma and red blood cell (RBC) were simultaneously determined. A blank plasma and RBC were initially run for baseline subtraction. Plasma and RBC samples were deproteinized with 6% PCA prior to HPLC. Following i.v. administration of PCr 500 mg x kg(-1) and 1 000 mg x kg(-1) the C-T curve could be described by the two-compartment model with t1/2beta 22.5-23.3 min, V(d) 0.956 4-0.978 6 L x kg(-1), CL 0.029 L. kg(-1) x min(-1). The Cr as PCr degraded product appeared as early as 2 min post i.v. dosing with t(max) 20 min, t1/2kappa (m) 40.6-42.7 min and f(m) 60%-76%. After po administration of PCr, the parent drug in plasma was undetectable, but the metabolite Cr was detected with t(max) 65-95 min, t1/2kappa (m) 56.0-57.7 min, metabolite-based bioavailability F(m) 55.02%-62.31%. PCr i.v. administration resulted in significant elevation of ATP level in RBC but not in plasma, the related-ATP in RBC was characterized by t(max) 68-83 min, t1/2kappa 49-52 min. In RBC no exogenous PCr was found but Cr was detected following i.v. administration of PCr, with the t(max) 120 min and t1/2k (m) 70 min for Cr. The above results indicate that PCr eliminates and bio-transforms in body very rapidly; K > K(m) confers ERL, instead of FRL, type upon the metabolic disposition of Cr. Following po administration of PCr, the degraded product Cr is absorbed but not the parent drug PCr. The formed Cr can be accounted for by most of i.v. and po PCr. Intravenous dosing leads apparently increased and sustained Cr and related-ATP concentration in RBC.


Subject(s)
Adenosine Triphosphate , Blood , Pharmacokinetics , Administration, Oral , Animals , Area Under Curve , Biological Availability , Biotransformation , Cardiotonic Agents , Blood , Pharmacokinetics , Creatine , Metabolism , Pharmacokinetics , Erythrocytes , Metabolism , Injections, Intravenous , Male , Phosphocreatine , Blood , Pharmacokinetics , Rats , Rats, Sprague-Dawley
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