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@#Abstract:Objective To improve the replication level of varicella⁃zoster virus(VZV)in human diploid cell line MRC⁃5 and increase the yield of VZV vaccine by reducing the expression of interferon(IFN)related genes via optimizing the cell line MRC⁃5. Methods Interferon receptor 1(IFNAR1)silenced MRC⁃5 cell line(MRC⁃5IFNAR1⁃)was constructed by CRISPR/Cas9 gene editing technology,which was determined for the relative expression of IFNAR1 mRNA,and for those of mRNA of IFN related genes IFNβ and OAS1 after VZV infection by qRT⁃PCR to evaluate the effect of gene silencing. Gene mutation sequences were further identified by sequencing of the silenced sites. The replication of VZV in MRC⁃5 and MRC⁃5IFNAR1⁃ cell lines was compared 168 h after VZV infection by using qRT⁃PCR and plaque formation unit(PFU)assay, to evaluate the effect of MRC⁃5IFNAR1⁃cell line on VZV replication. Results The growth status of MRC⁃5IFNAR1⁃ cell line wasconsistent with that of MRC ⁃ 5 cells,and the relative expression of IFNAR1 mRNA decreased by 73%;The relative expressions of IFNβ and OAS1 mRNA in MRC⁃5IFNAR1⁃ cell line were 61% and 90% lower than those in MRC⁃ 5 cells respectively after VZV infection;In addition,168 h after VZV infection,the level of DNA replication and the titer of VZV increased by 5. 7 folds and 4 folds respectively. Conclusion The successful establishment of MRC⁃5IFNAR1⁃ cell line may be a potential scheme to increase the yield of vaccines based on human diploid cells,and provided a reference for expanding production of VZV vaccine.
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@#Objective - - (BCL2L2)- ( ) To investigate the differential expression of the fusion gene BCL 2 like protein 2 poly A (PABPN1) ( ) binding protein nuclear 1 induced by sodium arsenite SA and its methylated metabolites in 16HBE cells and the Methods ) , related mechanism. i The 16HBE cells exposed to SA at concentrations of 1.5 3.0 and 4.5 µmol/L were set as -, - - low medium and high dose arsenic exposure groups. The 16HBE cells exposed to 4.5 µmol/L monomethylarsonic acid ( ), ( ) , MMA dimethylarsonic acid DMA and SA were set as MMA group DMA group and SA group. The 16HBE cells without , BCL2L2-PABPN1 toxic stimulation were set as control group. After the cells were cultured for 48 hours the expression of was - ( - ) ) ( ) detected by quantitative real time polymerase chain reaction qRT PCR . ii Two small interfering RNA siRNA silencing 基金项目:国家自然科学基金( ); 年云南省科技厅昆明医科大学应用基础研究联合专项面上项目 82160607 2021 ( ) 202101AY070001-054 作者简介:施雅( —),女,在读大学本科生,主要从事劳动卫生与环境卫生学研究;尹锦瑶( —),女,在读劳动卫生与环境卫 2001 1995 生学硕士研究生,主要从事劳动卫生与环境卫生学研究;施雅和尹锦瑶为共同第一作者 通讯作者:何越峰教授,博士研究生导师,- : E mail heyuefeng@kmmu.edu.cn中国职业医学 年 月第 卷第 期 , , , · · 2022 10 49 5 Chin Occup Med October 2022 Vol.49 No.5 523 BCL2L2-PABPN1, - fragments were designed and transfected into 16HBE cells to knockdown which were set as siRNA 1 group - - BCL2L2-PABPN1 and siRNA 2 group. Non transfected control group without knockdown of transfection was set up. After , BCL2L2-PABPN1 - culturing for 48 hours the expression level of in the three groups of cells was detected by qRT PCR. The cell - survival rate and early apoptosis rate were detected by MTS method and JC 1 mitochondrial membrane potential detection , ( ) , method respectively. The apoptosis was detected by Hoechest33342/propidium iodide PI double staining and the expression - Results ) level of P53 signaling pathway related proteins was detected by Western blotting. i The relative expression of BCL2L2-PABPN1 (P ) BCL2L2- in 16HBE cells increased with the increasing SA doses <0.01 . The relative expression of PABPN1 - , - - in high dose arsenic exposure was higher than that in control group low dose and medium dose arsenic exposure ( P ) BCL2L2-PABPN1 , groups all <0.05 . The relative expression of in SA group was higher than those in control group MMA ( P ) BCL2L2-PABPN1 group and DMA group all <0.05 . The relative expression of showed no significant difference between , ( P ) ) BCL2L2-PABPN1 control group MMA group and DMA group all >0.05 . ii The relative expression levels of and cell - - - ( P ) survival rate in siRNA 1 group and siRNA 2 group were lower than those in non transfected control group all <0.05 . , (P ) However there was no significant difference in the early apoptosis rate among the three groups >0.05 . The results of - Hoechest33342/PI double staining showed that the number of nuclear shrinkage and early apoptotic cells in siRNA 1 group and - - , - siRNA 2 group was higher than that in non transfected control group. The relative protein expression levels of P53 phospho , - - , - - ( P ) p53 BCL 2 associated death promoter P21 and cytochrome C in siRNA 1 group and siRNA 2 group were higher all <0.05 , - - P and the relative protein expression levels of P53 up regulated modulator of apoptosis were lower (all <0.05), when compared - Conclusion with the non transfected control group. SA may block the apoptosis of 16HBE cells by inducing the expression of BCL2L2-PABPN1 fusion gene . The mechanism may be related to the activation of P53 signaling pathway. The SA methylated BCL2L2-PABPN1 BCL2L2-PABPN1 - metabolites MMD and DMA had no effect on the expression of . may affect anti apoptosis BCL2L2 PABPN1 through affecting the synergistic effect of and genes.
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@#Objective The predisposing factors,clinical characteristics and imaging characteristics of intracranial and extracranial vertebral artery dissection are compared to understand the influence on the location of vertebral artery dissection and the ultrasonic characteristics of different parts.Methods Forty-four patients with vertebral artery dissection diagnosed in the Department of Neurology of our hospital from June 2017 to June 2021 were selected and divided into intracranial group and extracranial group according to different lesion sites of vertebral artery dissection.The differences in baseline data,susceptibility factors,clinical manifestations and ultrasonic characteristics of the two groups were compared.Results A total of forty-four patients were enrolled and were divided into intracranial vertebral artery dissection group (n=20 cases) and extracranial artery dissection group (n=24 cases).The incidence of massage or trauma in extracranial was significantly higher than that in intracranial (P=0.01).Headache symptoms were more common in intracranial group (P=0.04),however there were no significant differences in clinical features such as dizziness,hemiplegia,dysphagia and Horner’s sign between the two groups (P>0.05).Intramural hematoma was the most common ultrasonic characteristics in patients with vertebral artery dissection,and Ultrasonography was significantly limited in the intracranial segment.It is simple to operate,non-invasive,inexpensive and easy to popularize.Conclusions The predisposing factors and clinical manifestations of vertebral artery dissection are not identical in different parts.
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Aim To investigate the effeets of prolifera¬tion and autophagy of BV2 eells in OGD/R models when the 18 ku transloeator protein( TSPO) was inhibi¬ted.Methods BV2 microglia were eultured in vitro and the model established by oxygen-glueose depriva- tion/reperfusion( OGD/R) , the eells were divided into eontrol group and OGD/R group, OGD/R + small hair¬pin RNA negative eontrol group ( OGD/R + NCshR- NA) , OGD/R + TSPO small hairpin RNA group (OGD/R + TSPOshRNA ).The expression of TSPO mRNA and TSPO protein were deteeted by qRT-PCR and Western blot, respectively.In order to study the effeet of TSPO on BV2 microglial eells in OGD/R inju¬ry and autophagy, the cell viability was tested by CCK- 8 assey, the cytotoxicity was deteeted by reactive oxy¬gen speeies ( ROS) , autophagy-related mRNA ( p62 mRNA, LC3B mRNA, Beolin-1 mRNA) expressions were detected by qRT-PCR, and the expression levels of autophagy -related proteins ( p62 , LC3 II /LC3 1 , Beclin-1 ) were detected by Western blot in each group.Result The expression of TSPO mRNA and protein increased significantly in OGD/R group while compared to control group, the cell death and cytotox¬icity increased significantly, the expression levels of LC3B mRNA and Beclin-1 mRNA increased, while the p62 mRNA decreased significantly, the levels of LC3 II/LC3 1 and Beclin-1 protein increased, the expres¬sion of p62 protein decreased significantly in OGD/R group, and the autophagy was activated; compared with OGD/R group, the different levels of cell viabili¬ty, cytotoxicity and autophagy in OGD/R + NCshRNA group were not statistically significant.But the survival rate of cells in OGD/R + TSPOshRNA group signifi¬cantly increased, the levels of cytotoxicity and autoph¬agy were significantly reduced.Conclusions The in¬hibition of TSPO has a significant protective effect on OGD/R injury model in BV2 microglial cells, which may be related to the inhibition of autophagy.
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@#AIM: To investigate and analyze the safety and clinical efficacy vitrectomy combined with scleral buckling in treatment of rhegmatogenous retinal detachment associated with choroidal detachment.<p>METHODS: Totally 19 patients(19 eyes)of rhegmatogenous retinal detachment associated with choroidal detachment treated by vitrectomy combined with scleral buckling in our hospital from January 2014 to September 2017 were retrospective analyzed. Silicone oil was removed from the vitreous cavity 3 to 12mo after operation. Retinal reattachment rate, intraocular pressure(IOP), visual acuity recovery and complications were observed.<p>RESULTS:The IOP in vitreous cavity filled with silicone oil at 3mo after operation(16.09±3.58mmHg)and 6mo after silicone oil removal(14.69±3.10mmHg)were higher than those before operation(6.78±1.90mmHg)(all <i>P</i><0.05). Six months after silicone oil removal, the visual acuity of 15 eyes was improved. No complications of low IOP and atrophy occurred after operation.<p>CONCLUSION: Vitrectomy combined with scleral buckling is relatively safe and effective in treatment of rhegmatogenous retinal detachment associated with choroidal detachment, with high retinal reattachment rate, fewer complications and low reoperation rate.
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Objective To investigate the protective effect and the underlying mechanism of water soluble coenzyme Q10 (CoQ10)against rotenone induced injury on PC12 cells model.Methods PC12 cells were cultured with rotenone,water-soluble CoQ1 0 was added to the culture media 3 hours prior to the rotenone incubation.We determined cell viability by CCK8;reactive oxygen species (ROS)was detected by spectrophotometer;and Bcl-2, Bax,active Caspase-3,Caspase-9 and apoptosis-inducing factor (AIF)were measured by Western blotting after 24-hour rotenone incubation.Results After the treatment by rotenone,cell viability decreased significantly (P<0.01)and ROS level increased (P<0.01).CoQ10 could improve PC12 cell viability (P<0.01)and reduce the level of ROS (P<0.01).Western blotting experiments showed that CoQ10 could reduce rotenone-induced Caspase-9 (P<0.05),active Caspase-3 (P<0.05)and Bax (P<0.01)expressions,increase the expression of Bcl-2 (P<0.01),and prevent nuclear translocation of AIF (P<0.05).Conclusion CoQ10 has a protective effect on rotenone-induced apoptosis in PC12 cells,the mechanism of which may be through scavenging ROS in cells;decreasing caspase-9 ,active caspase-3 and Bax expressions;and increasing the expression of Bcl-2 ;and preventing AIF nuclear translocation.
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BACKGROUND: Skeletal muscle system is the driving force to reveal the change of human body movement, and its modeling is the key technology of human body modeling. OBJECTIVE: To establish models of skeletal muscle of human lower limb based on UG and ADAMS. METHODS: Body size parameters of the lower limbs of six men were measured. According to the collected motion parameters of the lower limbs, ground reaction force (ground support) and electromyogram signal of lower limb muscle group, ADAMS with the dynamics and kinematics simulation was conducted to get simulation parameters, such as ground reaction force, joint force, and link point displacement. Simulation value was compared with the measured value. Finally, MATLAB was used for simulation analysis. RESULTS AND CONCLUSION: It is concluded that the measured ground reaction force, joint angles, and link point displacement had very significant linear correlation with ADAMS simulation data. The simulation results show that a model of human lower limb skeletal muscle established by UG and ADAMS can effectively solve the tedious multi-rigid-body dynamics problems through the simulation calculation.
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@#AIM: To investigate the causes and effective diagnosis and treatment of Descemet's membrane detachment in the patients received phacoemulsification combined with intraocular lens implantation and extracapsular cataract extraction combined with intraocular lens implantation. <p>METHODS: A retrospective analysis was applied for 2 069 eyes in 2006 patients which had received one of above-mentioned surgeries from January 2015 to December 2017. The treatment and prognosis of 26 patients(26 eyes)who had the complication of Descemet's membrane detachment during or after the surgery were observed. <p>RESULTS:After the appropriate treatment, no corneal endothelial decompensation happened in the 26 patients, and the visual acuity was improved to different extent. UBM confirmed that the Descemet's membrane was reset. <p>CONCLUSION: Early detection and appropriate treatment of Descemet's membrane detachment is important for the visual acuity recovery.
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Abstract Chaetoglobosin A is an antibacterial compound produced by Chaetomium globosum, with potential application as a biopesticide and cancer treatment drug. The aim of this study was to evaluate the feasibility of utilizing cornstalks to produce chaetoglobosin A by C. globosum W7 in solid-batch fermentation and to determine an optimal method for purification of the products. The output of chaetoglobosin A from the cornstalks was 0.34 mg/g, and its content in the crude extract was 4.80%. Purification conditions were optimized to increase the content of chaetoglobosin A in the crude extract, including the extract solvent, temperature, and pH value. The optimum process conditions were found to be acetone as the extractant, under room temperature, and at a pH value of 13. Under these conditions, a production process of the antifungal chaetoglobosin A was established, and the content reached 19.17%. Through further verification, cornstalks could replace crops for the production of chaetoglobosin A using this new production process. Moreover, the purified products showed great inhibition against Rhizoctonia solani, with chaetoglobosin A confirmed as the main effective constituent (IC50 = 3.88 µg/mL). Collectively, these results demonstrate the feasibility of using cornstalks to synthesize chaetoglobosin A and that the production process established in this study was effective.
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Industrial Microbiology/methods , Callosities/microbiology , Chaetomium/metabolism , Indole Alkaloids/metabolism , Antifungal Agents/metabolism , Waste Products/analysis , Industrial Microbiology/instrumentation , Callosities/metabolism , Molecular Structure , Plant Stems/metabolism , Plant Stems/microbiology , Indole Alkaloids/isolation & purification , Indole Alkaloids/chemistry , Antifungal Agents/isolation & purification , Antifungal Agents/chemistryABSTRACT
To investigate the effect and mechanism of Dracocephalum moldovica total flavones (TFDM) on the formation of atherosclerosis ApoE-/- mice induced by high fat diet. A total of 40 SPF 8-week-old male ApoE-/- mice were fed with high fat diet and randomly divided into 5 groups. TFDM high, medium, low-dose group were given 21, 42, 84 mg•kg⁻¹•d⁻¹ by gavage; Simvastatin group was fed with simvastatin 3.5 mg•kg⁻¹•d⁻¹; and model group was given the same dose of normal saline. The other eight male C57BL/6J mice of the same genetic background and age were set up as control group and fed with common diet. All of the groups were intragastrically intervened for 12 weeks. The aortic pathologic changes were observed with HE; qRT-PCR was adopted to detect TGF-β1, Smad2, Smad3, MMP-2 and MMP-9 gene levels in tissues. Compared with model group, HE staining in TFDM group showed obvious relief of aortic atherosclerotic tissue injury; each TFDM group showed inhibition in mRNA expressions of TGF-β1, Smad2, Smad3, MMP-2 and MMP-9. This suggests that TFDM can inhibit atherosclerosis formation, which may be related to the intervention of TGF-β1/Smads signal transduction.
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Objective To explore the changes in inflammatory reactions in tail-suspension mice infected by Klebsiella pneumoniae from spaceflight.Methods Tail suspension was used to simulate the physiological effects of microgravity.C57BL/6 mice were randomly divided into control (Con),control+K.pneumoniae T16-169 (Con+T16-169),tail suspension (TS) and tail suspension+K.pneumoniae T16-169 (TS+T16-169) groups.The level of inflammatory cytokines TNF-α,IL-6 and IL-1β mRNA in lung tissue and the plasma cytokine concentration were detected by RT-qPCR and xMAP technology,and HE staining was used to represent the morphological changes in lung tissue.Results Compared with the control group,the expression of inflammatory cytokines in lung tissue and plasma concentrations of all experimental groups were increased,and the difference in TS+T16-169 group was the most significant (P<0.01 or P<0.001).HE staining showed that the lung tissues in Con+T16-169 and TS+T16-169 groups were damaged in different degrees,and the damage of TS+T16-169 group was the most serious.Conclusion The K.pneumoniae from spaceflight significantly increases the expression of inflammatory cytokines in lung tissue and plasma concentrations after infecting tail-suspension mice,and induces more serious damages to the lung tissue,which suggests that inflammatory reactions can be increased in tail suspension mice infected by K.pneumoniae from spaceflight.
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Objective To construct the eukaryotic expression vector pEGFP-N1/IL-37b and analyze the expression of IL-37 gene in THP-1 cells. Methods Total RNA was extracted from human peripheral blood mononuclear cells ( PB-MCs) and the coding region of IL-37b gene was amplified by RT-qPCR. Then, the gene was cloned into pEGFP-N1 eu-karyotic expression vector. After transfected the recombinant plasmid into THP-1 cells, the expression of IL-37 was detec-ted by RT-qPCR and Western blot. Results Double restriction enzyme digestion and gene sequencing showed that IL-37b gene was correctly inserted into the eukaryotic expression vector pEGFP-N1. RT-qPCR and Western blot showed that the IL-37 expression level was increased significantly (P<0. 01) after transfection in THP-1 cells. Conclusions We successful-ly constructed a novel anti-inflammatory cytokine IL-37 eukaryotic expression vector pEGFP-N1/IL-37b, which lays a foun-dation for further study on IL-37 functions and its association with related diseases.
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Objective To explore serum IL-6 (interleukin-6) ,IL-10 (interleukin-10), the WBC (white blood cell count), CRP (C-reactive protein)and PCT ( procalcitonin ) level in early diagnosing of in children with hand, foot and mouth disease and bacterial infection. Methods 63 children in bacterial infection group and virus infection group respectively,collected 63 children in normal control group by physical examination. Enzyme-linked immunosorbent assay (ELISA) double antibody clamp method was used to determine serum IL-6 and IL-10 level. Immune turbidimetric , enzyme-linked fluorescent were used to detected serum CRP , PCT. WBC results were also compared. Resluts Serum IL-6, IL-10, CRP and PCT level were higher in normal control group compared with virus infection group , the WBC level was slightly lower than normal control grou with statistically different IL-6, IL-10, CRP level (P 0.05). Conclusion IL-6, IL-10 levels increased significantly in the early stage of hand,foot and mouth disease, but in the aspect of combination of bacterial infection and bacterial infection, their clinical value was not so significant. WBC, CRP and PCT level had important value for early diagnosis in hand, foot and mouth disease with bacterial infection.
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Objective To learn the epidemiological characteristics of syphilis in Shihezi in recent three years and to provide bases for prevention .Epidemiological analysis with syphilis was conducted in our hospital from 2012 to 2014 ,which is about the detection rate ,age ,gender ,and the distribution in the department .Methods Serum were deteceted by using three methods and the data were analyzed .Results 1 281 syphilis cases in 74 798 patients were detected in our hospital during this period .The total positve rate of three years was 1 .71% .The positve rate was 1 .35% ,1 .83% and 2 .01% respectively .The results of three years was significant differences(χ2 =39 .877 ,P0 .05) .The ca‐ses were mainly distributed in Han(581 ,45 .36% ) ,Uygur(43 ,3 .36% ) ,Kazak(23 ,1 .80% ) .353 cases were negative by RPR among 1 281 patients with syphilis(27 .56% ) ,and the negative rises year by year .Conclusion The incidence of syphilis increased slightly in Shihezi since 2012 .Though the detection rate between 2013 and 2014 was no significant difference .The focus was on 20- year′s old women of childbearing age .It is necessary to take strict measures to control the spread of syphilis and to do syphilis examina‐tion for all inpatients in order to prevent the infection of syphilis .
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Objective To understand the prevalence rate and related factors of chronic kidney disease (CKD) among elderly people aged more than 65 years old in the 66th regiment of the fourth division of A Crops in Xinjiang .Methods A total of 2 030 elderly people aged more than 65 years old in the 66th regiment of the fourth division of XPCC were distributed in 6 communities . Totally 334 permanent residents aged more than 65 years old were chosen from 2 communities by the stratified random sampling method .The renal injury indicators and related factors were detected .Results Among 329 residents with intact data ,after the age correction ,the prevalence rate of albuminuria ,hematuria and renal function decrease were 22 .2% ,14 .2% ,4 .9% ,respectively .The prevalence rate of CKD in this group was 32 .8% ,CKD stage 1―3 were dominated .The awareness rate was 15 .1% .The multiva‐riate Logistic regression analysis showed that gender and hypertension were independently associated with CKD .Conclusion The prevalence rate of CKD among elderly people aged over 65 years old in the 66th regiment of the fourth division of this Crops is high‐er .The related factors are gender and hypertension .
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The chemical structures of P1 A was identified by complete acid hydrolysis, partial acid hydrolysis, periodate oxidation-Smith degradation, methylation analysis, IR and NMR. The results showed that P1 A had a backbone consisting rhamnose, mannose, glucose and galactose. The side chain possessed arabinose and xylose. 1-->, 1-->6 and non-reducing terminal linkages existed in polysaccharide P1A, but there are doubling amount of 1-->2 and 1-->4 linkages. Oxidable linkage of P1 A accounted for 45%, and inoxidable linkage of P1A accounted for 55%. Mannose, glucose and galactose were mainly linked by 1-->2 linkage. Rhamnose, arabinose and xylose were mainly linked by 1-->2 and 1-->4 linkages. PlA contained beta-Glc(1,6)-,beta-Gal(1,3)-,beta-Man(1,4)-beta-Rha,-Glc(1,4)-, Glc(1)-,-Gal(1,4)- and Man(1)-.
Subject(s)
Acanthaceae , Chemistry , Drugs, Chinese Herbal , Chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Molecular Weight , Polysaccharides , ChemistryABSTRACT
Objective To understand the prevalence situation of common respiratory tract pathogens in Shihezi area to provide reliable basis for clinical diagnosis and treatment Methods The serum samples from the inpatients with acute respiratory tract in‐fection in the First Affiliated Hospital of Medical College of Shihezi University from January to June 2014 were collected and detec‐ted 9 kinds of common pathogens by using the indirect immuno‐fluorescence assay .Results Among 810 serum samples ,the IgM antibody positive detection rate was 32 .35% ,the detection rates of various pathogens from high to low were Mycoplasma pneumon‐iae(MP ,21 .48% ) ,Q fever rickettsia (COX ,8 .8% ) ,legionella pneumophila(LP1 ,5 .18% ) ,Chlamydia pneumoniae(CP ,4 .2% ) ,in‐fluenzaBvirus(INFB,2.22% ),parainfluenzavirus(PIVs,1.24% )andrespiratorysyncytialvirus(RSV,0.50% ).MPinfectionwas dominated by young children ,the detection rate in females was higher than that in males (P<0 .05);majority of COX infection were young adults ,the detection rate in males was higher than that in females (P<0 .05) .Conclusion MP is the main respiratory tract infection pathogen in children and COX is the main respiratory tract infection pathogen among young adults in Shihezi area .
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Objective To investigate the association between plasma homocysteine (Hcy) levels and cystathionineβsynthase (CBS) T833C gene polymorphism with essential hypertension in Xinjiang Kazakh and Han populations. Methods A total of 239 Kazak patients with hypertension (Kazak EH group), 206 Kazak people with normal blood pressure (Kazak con?trol group), 256 Han patients with hypertension (Han EH group) and 206 Han people with normal blood pressure (Han con?trol group) were selected for the study. Amplification refractory mutation system(ARMS) was used to analyze the polymor?phism of CBS gene T833C,TT,TC and CC genotypes and the various sites of T,C allele frequencies in four groups. In the meantime, the Hcy level and related biochemical indices were detected using automatic biochemical analyzer. Results The plasma Hcy levels were significantly higher in Kazak EH group and Han EH group than those of Kazak control group and Han control group (P0.05).Conclusion The Cystathionineβsynthase gene of T833C polymorphism may be associated with essential hypertension in Kazak people in Xinjiang, but no such association in Han population in Xinji?ang. The mechanism may be related to the altered metabolism of Hcy induced by CBS mutation.
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Objective To evaluate the clinical application value of flow fluorescence assay in determination of serum pepsinogenⅠ and Ⅱ.Methods The precision of flow fluorescence assay in the detection of serum pepsinogen Ⅰ and Ⅱ were evaluated. Methodology comparison were conducted between flow fluorescence assay and enzyme-linked immunosorbent assay(ELISA)kit through detecting clinical samples.The reference ranges for PGⅠ and PGⅡ/PGⅠ ratio of healthy population were established. The levels of PGⅠand PGⅡ/PGⅠ ratio were detected in serum samples of patients suffering superficial gastritis,atrophic gastritis and gastric cancer.Results The within-run and between-run coefficient of variation of PGⅠ were 4.26%-5.35% and 6.73%-7.75%,respectively.And those of PGⅡ were 5.48% - 6.42% and 8.46% -8.85%,respectively.Methodology comparison be-tween flow fluorescence assay and ELISA demonstrated good linear correlations.The linear equation wasY =0.91 1X -22.635(r=0.966,P <0.05)and Y =0.892X -0.548(r=0.980,P <0.05)for PGⅠ and PGⅡ,respectively.The lower limit of the reference range of PGⅠ and PGⅡ/PGⅠ ratio were 32.77 ng/mL and 4.1 6,respectively.The PGⅠ and PGⅠ /PGⅡ ratio of patients suf-fering atrophic gastritis and gastric cancer were statistically significantly lower than those in patients suffering superficial gastritis (P <0.05).Conclusion The flow fluorescence assay could conduct simultaneous detection of PGⅠ and PGⅡwith good methodo-logical performance and high efficiency.The determination of PGⅠ and PGⅡlevels through flow fluorescence assay could provide laboratory basis for the screening and diagnosis of atrophic gastritis and early gastric cancer.
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Objective To investigate the correlation between the plasm homocysteine (Hcy) levels and cystathionine β synthase (CBS) T833C gene polymorphism in Xinjiang Kazakh with essential hypertension. Methods 239 Kazak patients with hypertension (hypertension group) and 206 with normotensive (control group) were selected for the study. Amplification Refractory Mutation System (ARMS) was used to analyze the polymorphism of CBS gene T833C, TT, TC and CC genotypes and the various sites of T, C allele frequencies in research group and the control group. Results Plasma of Hcy level was higher in the hypertensive group than those of control group, the difference was significant (P<0.05), and the individual plasma of Hcy with TC gene was higher than that with TT gene. The C allele frequencies was significantly higher in EH group than that in controls in Xinjiang Kazakh population, and the difference was significant (P<0.05). The risk of EH group in individuals carrying TC genetype was 2.39 times higher than in individuals carrying TT genetype (OR = 2.39, 95%CI:1.125 ~5.076, P = 0.02). Conclusion Elevated Hcy level may be a risk factor of Kazakhs hypertension in Xinjiang. The Cystathionine β synthase gene of T833C polymorphism may be associated with essential hypertension in Kazak people in Xinjiang.