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1.
Protein & Cell ; (12): 26-46, 2022.
Article in English | WPRIM | ID: wpr-922494

ABSTRACT

In vitro studies have established the prevalent theory that the mitochondrial kinase PINK1 protects neurodegeneration by removing damaged mitochondria in Parkinson's disease (PD). However, difficulty in detecting endogenous PINK1 protein in rodent brains and cell lines has prevented the rigorous investigation of the in vivo role of PINK1. Here we report that PINK1 kinase form is selectively expressed in the human and monkey brains. CRISPR/Cas9-mediated deficiency of PINK1 causes similar neurodegeneration in the brains of fetal and adult monkeys as well as cultured monkey neurons without affecting mitochondrial protein expression and morphology. Importantly, PINK1 mutations in the primate brain and human cells reduce protein phosphorylation that is important for neuronal function and survival. Our findings suggest that PINK1 kinase activity rather than its mitochondrial function is essential for the neuronal survival in the primate brains and that its kinase dysfunction could be involved in the pathogenesis of PD.

2.
Journal of Clinical Hepatology ; (12): 477-482, 2022.
Article in Chinese | WPRIM | ID: wpr-920917

ABSTRACT

Primary intrahepatic stones (PIS) is a refractory disease with a high incidence rate in southwest China, and some patients still require surgery again or even more times after initial treatment. Many studies in recent years have shown that some specific flora can colonize in the intrahepatic bile duct, leading to chronic infection and inflammation of the biliary system, and these specific types of flora, called "stone-causing flora", can produce metabolites such as β-glucuronidase and play an important role in the formation of pigmented stones. This article analyzes the role of stone-causing flora in the pathogenesis of PIS, so as to provide more treatment options for PIS patients.

3.
Journal of Clinical Hepatology ; (12): 402-408, 2022.
Article in Chinese | WPRIM | ID: wpr-920894

ABSTRACT

Objective To investigate the risk factors for early-stage complications among liver transplant recipients, and to establish and validate a risk prediction model for early-stage complications after transplantation. Methods A retrospective analysis was performed for the clinical data of 234 patients who underwent orthotopic liver transplantation in Department of Liver Transplantation, Tianjin First Central Hospital, from January 2016 to December 2018. According to the presence or absence of Clavien-Dindo grade ≥Ⅲ complications after liver transplantation, the patients were divided into complication group with 97 patients and non-complication group with 137 patients. The two groups were compared in terms of the indices including age, sex, body mass index (BMI), blood type, psoas muscle thickness/height (PMTH), Controlling Nutritional Status (CONUT) score, Model for End-Stage Liver Disease (MELD) score, total serum bilirubin, serum creatinine, international normalized ratio of prothrombin time, blood urea nitrogen, hemoglobin, white blood cell count, platelet count, amount of intraoperative red blood cell transfusion, amount of frozen plasma transfusion, blood loss, anhepatic phase, time of operation, donor age, donor BMI, cold ischemia time of donor liver, and warm ischemia time of donor liver. The independent samples t -test was used for comparison of normally distributed continuous data between two groups, and the Mann-Whitney U test was used for comparison of non-normally distributed continuous data between two groups; the chi-square test was used for comparison of categorical data between two groups. Univariate analysis and the binary logistic regression analysis were used to investigate the risk factors for early-stage complications after liver transplantation, and a risk prediction model for complications after liver transplantation was established based on the method for establishing a scoring system using the logistic model provided by Framingham Research Center. Internal validation of the model was performed by C-index, receiver operating characteristic (ROC) curve, calibration curve, and the Hosmer-Lemeshow test, and the decision curve was used to evaluate the clinical applicability of the model. The Kaplan-Meier method was used to compare the incidence rate of early-stage complications after liver transplantation between the patients with different risk scores. Results Compared with the non-complication group, the complication group had significantly higher MELD score, proportion of patients with low PMTH, total serum bilirubin, serum creatinine, blood urea nitrogen, CONUT score, amount of intraoperative red blood cell transfusion, and amount of frozen plasma transfusion, as well as a significantly lower level of hemoglobin (all P < 0.1). The multivariate binary logistic regression analysis showed that MELD score (odds ratio [ OR ]=1.104, 95% confidence interval [ CI ]: 1.057-1.154, P < 0.05), PMTH ( OR =2.858, 95% CI : 1.451-5.626, P < 0.05), and CONUT score ( OR =1.481, 95% CI : 1.287-1.703, P < 0.05) were independent risk factors for grade ≥Ⅲ complications in the early stage after liver transplantation. MELD score, PMTH, and CONUT score were included in a predictive model, and this model had the highest score of 24 points, a C-index of 0.828, an area under the ROC curve of 0.812( P < 0.001), a sensitivity of 0.792, and a specificity of 0.751, suggesting that this predictive model had good discriminatory ability. The calibration curve of this model was close to the reference curve, and the Hosmer-Lemeshow test obtained a chi-square value of 8.528( P =0.382), suggesting that this predictive model had a high degree of fitting. The decision curve showed that most patients were able to benefit from the predictive model and achieved a high net benefit rate, suggesting that this predictive model had good clinical applicability. The score of 11 was selected as the cut-off value according to the optimal Youden index of 0.507, and the patients were divided into low-risk (< 8 points) group with 55 patients, moderate-risk (8-10 points) group with 63 patients, high-risk (11-14 points) group with 67 patients, and extremely high-risk (≥15 points) group with 49 patients. These four groups had a 90-day cumulative incidence rate of early-stage postoperative complications of 3.6%, 28.6%, 59.7%, and 75.5%, respectively, and the incidence rate of complications increased with the increase in risk score ( P < 0.001). Conclusion MELD score, PMTH, and CONUT score are independent risk factors for early-stage complications among liver transplant recipients, and the risk prediction model established based on these factors has a high predictive value in high-risk patients.

4.
China Pharmacy ; (12): 446-451, 2022.
Article in Chinese | WPRIM | ID: wpr-920461

ABSTRACT

OBJECTIVE To investigate the component characteristics and anti-infla mmatory activities of the low polarity parts from Sabia campanulata ,S. discolor and S. parviflora . METHODS Gas chromatography-mass spectrometry was used to analyze the components of low polarity parts eluted by petroleum ether from petroleum ether extract of dried stems and leaves of S. campanulata,S. discolor and S. parviflora . The chromatographic peaks were retrieved by both NIST 17 and Wiley 275 database, and their structures of each compound were compared and identified manually. The relative percentage content of each component was calculated by peak area normalization method. With indomethacin as positive control and the inhibition rate of nitric oxide (NO)production as the index ,the effects of the low polarity parts of the above three species of Sabia genus on the production of NO in lipopolysaccharide-induced human rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) and mouse mononuclear macrophage RAW 264.7 were compared ;the half inhibitory concentration (IC50)was calculated. RESULTS Totally 70,40 and 33 chemical components were identified respectively from the low polarity parts of S. campanulata ,S. discolor and S. parviflora , including long-chain fatty hydrocarbons ,long-chain alcohol/aldehyde/ester ,monoterpenes;but there were only 5 common components in the three species ,such as dodecane and docosane,etc. S. campanulata contained abundant terpenoids (40.89%); the relative percentage content of triterpenoids was high in S. discolor (30.29%);the relative percentage content of long-chain fatty hydrocarbons was high in S. parviflora (87.70%). The values of IC 50 of the low polarity parts of the above three species of Sabia genus to the production of NO in RA-FLS cells were 2.22,1.69 and 4.15 μg/mL,respectively. The values of IC 50 of them to the production of NO in RAW 264.7 cells were 1.23,4.02 and 3.05 μg/mL respectively,and all of them were lower than indomethacin (12.97,10.66 μg/mL). CONCLUSIONS The components of the low polarity parts fr om the above three species of Sabia genus are mainly long-chain fatty hydrocarbons , monoterpenes and long-chain alcohol/aldehyde/ester , and all of them have certain anti-inflammatory activities.

5.
Journal of Clinical Hepatology ; (12): 2967-2971, 2021.
Article in Chinese | WPRIM | ID: wpr-906900

ABSTRACT

With the increasing demand for liver donors, more and more marginal donors have been used. How to use fatty liver grafts has become a new issue that physicians in the department of liver transplantation must face. Compared with the normal donor liver, fatty liver grafts have high incidence of postoperative complications and increase the difficulty of clinical cure. This review describes the measures to improve the prognosis of recipients of fatty liver transplantation, and points out that these measures can reduce the risk of fatty liver transplantation and thus increase the utilization of fatty liver grafts.

6.
Acta Pharmaceutica Sinica ; (12): 3441-3450, 2021.
Article in Chinese | WPRIM | ID: wpr-906822

ABSTRACT

Outer membrane vesicles (OMVs) are nano-sized spherical vehicles, with a size range between 20-250 nm. OMVs are spontaneously secreted from Gram-negative bacteria and formed by lipid bilayer membranes, comprising multiple parent bacteria-derived components including bacterial antigens, pathogen-associated molecular patterns, proteins and lipids. OMVs have shown multiple potentials for the treatment of various diseases, including cancer therapy and bacterial infection. In this review, the structure, composition and methods for isolating and characterizing of OMVs were introduced. The applications of OMVs for diseases therapy were summarized and future perspectives were discussed.

7.
Article in Chinese | WPRIM | ID: wpr-906228

ABSTRACT

Danggui Liuhuang Tang, as one of classical traditional Chinese medicine prescriptions, has been used by major medical experts in clinic since Jin and Yuan dynasties. After review and summarization of relevant literatures on the pharmacological effects and clinical application of Danggui Liuhuang Tang, it was found that Danggui Liuhuang Tang has a wide range of pharmacological activities, and exerts its anti-inflammatory and anti-insulin resistance effects mainly by inhibiting the production of inflammatory factors, such as tumor necrosis factor and interleukin and activating related pathways. In addition, Danggui Liuhuang Tang inhibits the occurrence and development of hepatic fibrosis by attenuating proinflammatory signaling and extracellular matrix accumulation with multiple components, multiple targets, and multiple pathways. Danggui Liuhuang Tang has been widely used in sweat syndromes, with an obvious effect in the treatment of thyroid diseases, diabetes, respiratory tract and other diseases. This paper reviews and summarizes the pharmacological effects and clinical application of Danggui Liuhuang Tang,in an attempt to provide some valuable clues for the subsequent development of Danggui Liuhuang Tang.

8.
Article in Chinese | WPRIM | ID: wpr-905073

ABSTRACT

Objective:To observe the efficacy of modified Huanglian Wendantang in treating newly diagnosed type 2 diabetes mellitus (T2DM) with phlegm (dampness)-heat syndrome, in order to study the effect on islet β cell function and adipocytokines. Method:A total of 130 patients were randomly divided into two groups by random number table (65 cases in each group). The 60 patients in control group completed the treatment (4 patients fell off or lost visit, 2 were eliminated because of breach of plan), and the 61 patients in observation group completed the treatment (3 patients fell off, 1 were eliminated). And 20 healthy volunteers were taken as normal control group. Both groups′ patients got lifestyle interventions and metformin hydrochloride tablets (1 tablet/time, 1 time/day during the meal). In addition, patients in control group got Huazhuo Qingshen Keli in the morning and at night, 5 g/time, 2 times/day, and patients in observation group got modified Huanglian Wendantang, 1 dose/day. And the treatment was lasted for 3 months. Before and after treatment, levels of fasting blood glucose (FBG), postprandial 2 blood glucose (PBG), HbA1c and fasting insulin (FINS), insulin resistance index (HOMA-IR), insulin sensitivity index (InISI), islet β cell function index (HOMA-β), early insulin secretion index (I30/△G30) and late insulin secretion index (AUCI30~I120/G30~G120), total cholesterol (TC) and triglycerides (TG), high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C), adiponectin, TNF -α (TNF-α), resistin and leptin were detected. And syndrome of phlegm (dampness) combined with heat were scored, and the safety was discussed. Result:The total effective rate in observation group was 91.80% (56/61), which was higher than 78.33% (47/60) in control group (χ2=4.333, P<0.05). And the score of phlegm (dampness)-heat syndrome was lower than that in control group (P<0.01), levels of FBG, PBG, HbA1c, HOMA-IR, AUCI30~I120/G30~G120, TC, TG, LDL-C, TNF-α, leptin and resistin were lower than those in control group (P<0.01), while levels of I30/△G30, HOMA-β, InISI, HDL-C and adiponectin were higher than those in control group (P<0.01). There was no adverse reaction related to modified Huanglian Wendantang. Conclusion:In addition to treatment with metformin, modified Huanglian Wendantang can effectively control blood glucose and lipid, regulate adipocyte factor, improve early and late phase insulin secretion, improve the function of β cell and insulin sensitivity of islet, improve IR, with a better comprehensive efficacy and a safety in clinical use.

9.
Article in Chinese | WPRIM | ID: wpr-912443

ABSTRACT

Advanced lipid testing is an extension of traditional standard blood lipid testing. These biomarkers have has been widely used in the risk assessment and differential diagnosis of atherosclerotic cardiovascular disease (ASCVD), especially for people with unclear diagnosis and other metabolic risk factors. However, the differences in the measurement results and traceability issues limit the widespread use of these biomarkers. This article aims to introduce and discuss the current status of clinical application of advanced lipid testing in risk assessment of ASCVD, the progress of detection technology and detection standardization in the field.

10.
Chinese Medical Journal ; (24): 1952-1958, 2021.
Article in English | WPRIM | ID: wpr-887625

ABSTRACT

BACKGROUND@#Early detection of gastric cancer (GC) has been the topic of major efforts in China. This study aimed to explore the risk factors associated with GC and to provide evidence for the selection of a high-risk population of GC.@*METHODS@#Based on the cancer screening cohort of the National Cancer Screening Program in Urban China, GC patients diagnosed by endoscopy and pathological examinations constituted the case group, and controls were 1:3 matched by sex and age (±5 years) individually. The variables were selected by univariable analysis of factors such as body mass index (BMI), dietary habits, lifestyle, stomach disease history, and family history of GC; and multivariable logistic regression was used to analyze the influencing factors of GC and to calculate the odds ratio (OR) of related factors and its 95% confidence interval (CI).@*RESULTS@#A total of 215 GC cases and 645 matched healthy controls were included in the final analysis, with a median age of 61 years for the case and control groups. Overall analysis showed that high educational level (above primary school) (OR = 0.362, 95% CI = 0.219-0.599, P < 0.001), overweight/obesity (BMI ≥24 kg/m2; OR = 0.489, 95% CI = 0.329-0.726, P < 0.001), cigarette smoking (OR = 3.069, 95% CI = 1.700-5.540, P < 0.001), alcohol consumption (OR = 1.661, 95% CI = 1.028-2.683, P = 0.038), history of stomach disease (OR = 6.917, 95% CI = 4.594-10.416, P < 0.001), and family history of GC in first-degree relatives (OR = 4.291, 95% CI = 1.661-11.084, P = 0.003) were significantly correlated with the occurrence of GC. Subgroup analyses by age and gender indicated that GC risk was still increased in the presence of a history of stomach disease. A history of chronic gastritis, gastric ulcer, or gastric polyposis was positively associated with GC, with adjusted ORs of 4.155 (95% CI = 2.711-6.368), 1.839 (95% CI = 1.028-3.288), and 2.752 (95% CI = 1.197-6.326).@*CONCLUSIONS@#Subjects who smoke, drink, with history of stomach disease and family history of GC in first-degree relatives are the high-risk populations for GC. Therefore, attention should be paid to these subjects for GC screening.


Subject(s)
Case-Control Studies , Humans , Middle Aged , Overweight , Risk Factors , Stomach Neoplasms/etiology
11.
Acta Pharmaceutica Sinica ; (12): 1070-1078, 2021.
Article in Chinese | WPRIM | ID: wpr-886981

ABSTRACT

Z-VAD-FMK was combined with hypoxia-reoxygenation (H/R) injury to establish a necroptosis model of H9c2 cells to mimic the pathological changes of myocardial ischemia reperfusion injury (MIRI) in vitro and to study the effect and mechanism of tilianin against myocardial ischemia-reperfusion injury. A cell counting kit-8 (CCK-8) was used to detect cell viability, and commercial kits were used to detect lactate dehydrogenase (LDH) and superoxide dismutase (SOD) in the cell culture supernatant. Hoechst 33342/PI immunofluorescence staining was used to detect cell death. DCFH-DA, BBcellProbeTMM61, and JC-1 probes were used to detect reactive oxygen species (ROS), mitochondrial permeability transition pore (mPTP), and mitochondrial membrane potential (MMP), respectively. An enzyme-linked immunosorbent assay (ELISA) method was used to detect the release of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6). The results show that the cell viability, SOD activity, and MMP of the model group induced by H/R injury decreased, as compared with control group, but the necroptosis rate, LDH level, and ROS release increased significantly. Furthermore, mPTP of the model group cells opened, and TNF-α, IL-1β, and IL-6 levels were significantly higher. Molecular docking modeling showed that tilianin can bind to calmodulin-dependent protein kinase II (CaMKII), and Western blot results showed that compared with control group, the expression levels of p-CaMKII and phospho-mixed lineage kinase domain-like protein increased in the model group, and tilianin could decrease the expression level of these proteins. The above results indicate that tilianin can protect H9c2 cells by inhibiting the phosphorylation of CaMKⅡ at threonine 287, protecting mitochondrial function, and inhibiting the opening of mPTP to prevent necroptosis. This study has value for research on new methods to treat H/R injury.

12.
Article in Chinese | WPRIM | ID: wpr-884196

ABSTRACT

Objective:To investigate the epidemiological and clinical features of patients with hemorrhagic fever with renal syndrome (HFRS) from 2017 to 2019.Methods:Seventy-five patients with HFRS from the Department of Infectious Diseases, The First Affiliated Hospital of Anhui Medical University during January 1, 2017 to December 31, 2019 were included. The data of epidemiology, clinical symptoms, blood routine, urine routine, serum creatinine, liver function and other laboratory examination indexes were retrospectively analyzed. The measurement data with skewness distribution were expressed by M( QR) and compared by nonparametric test. Multivariate logistic regression analysis was used to analyze disease-related risk factors. Results:The 75 patients were mainly located in the western and northern regions of Anhui Province. A total of 37 cases (49.3%) were infected during November, December and January next year. Fifty-four (72.0%) patients were farmers and 10(13.3%) patients had a clear history of rodent contact. Only 19(25.3%) patients had typical clinical manifestations of "three red and three pain" . Fifty-eight (77.3%) patients had elevated white blood cell count, 67(89.3%) patients had decreased platelet count, 55(73.3%) patients had urinary protein + + + , 65(86.7%) patients had abnormal urinary occult blood, and 67(89.3%) patients had elevated serum creatinine. The serum creatinine and potassium levels in 31 severe and critical patients were 495(301) μmol/L and 4.14(0.77) mmol/L, respectively, which were both higher than those in 44 mild and moderate patients (235(289) μmol/L and 3.65(1.02) mmol/L, respectively). The differences were both statistically significant ( Z=-3.187 and -2.796, respectively, both P<0.01). Multivariate logistic regression analysis showed that serum creatinine (odds ratio ( OR)=1.005, 95% confidence interval ( CI)1.002-1.008) and serum potassium ( OR=2.632, 95% CI 1.098-6.313) were independent risk factors for disease severity. All patients received comprehensive medical treatment, and 27 patients received renal replacement therapy. Sixty-eight patients had good prognosis and four patients died. Conclusions:HFRS is still common in the rural area in winter and spring. Patients with atypical clinical manifestations and severe and critical patients should be intensively monitored.

13.
Clinical Medicine of China ; (12): 193-207, 2021.
Article in Chinese | WPRIM | ID: wpr-884170

ABSTRACT

In China, the malignant tumor with the highest incidence and motality is lung cancer (LC). As screening and early detection and treatment are effective in reducing LC mortality, formulating a guideline in line with China′s national conditions for the screening and early detection and treatment of LC will greatly promote the homogeneity and accuracy of LC screening, and result in an improvement of the effectiveness of LC screening. Commissioned and directed by the Disease Prevention and Control Bureau of the National Health Commission of the People′s Republic of China, the guidline was initiated by the National Cancer Center of China and formulated with joint effort by experts from different disciplines. Following the principles and methods in WHO Handbook for Guideline Development, the guidline integrates the latest development in LC screening and early diagnosis and treatment worldwide while fully considering China′s national conditions and practical experience in LC screening. It provides detailed evidence-based recommendations for different aspects of LC screening, such as the targeted population, the technologies and the procedures, to regulate the practices of LC screening and early diagnosis and treatment and enhance the effectiveness of the prevention and control of LC in China.

14.
Article in Chinese | WPRIM | ID: wpr-878434

ABSTRACT

Bone invasion by oral cancer is a common clinical problem, which affects the choice of treatment and predicts a poor prognosis. Unfortunately, the molecular mechanism of this phenomenon has not been fully elucidated. Current studies have revealed that oral cancer cells modulate the formation and function of osteoclasts through the expression of a series of signal molecules. Many signal pathways are involved in this process, of which receptor activator of nuclear factor-κB ligand/receptor activator of nuclear factor-κB/osteoprotegerin signaling pathway attracted much attention. In this review, we introduce recent progress in molecular mechanisms of bone invasion by oral cancer.


Subject(s)
Bone Resorption , Bone and Bones , Humans , Mouth Neoplasms , Osteoclasts , Osteoprotegerin , RANK Ligand , Receptor Activator of Nuclear Factor-kappa B
15.
Article in Chinese | WPRIM | ID: wpr-878433

ABSTRACT

Photodynamic therapy (PDT) has developed rapidly in basic and clinical research, and its therapeutic prospects have received increasing attention. PDT has the advantages of minimally invasive, low toxicity, high selectivity, good reproducibility, protection of appearance and vital organ function, and has become a treatment. With the development of medicine, the field of application of PDT becomes more wildly, and brings a new direction for the treatment of oral diseases. This article reviews the basic principles, treatment elements and research results of PDT in the treatment of oral diseases.


Subject(s)
Humans , Mouth Diseases/drug therapy , Photochemotherapy , Photosensitizing Agents/therapeutic use , Reproducibility of Results
16.
Article in English | WPRIM | ID: wpr-878416

ABSTRACT

Oral squamous cell carcinoma (OSCC) is the most frequent tumour in head and neck malignant. The current treatment is mainly based on surgery therapy, radiation therapy and chemical therapy. Meanwhile, there are many a defect in the treatment. For example, there are many defects in radiotherapy. Radioactive salivatitis is the most common. In addition, there are a series of changes such as dry mouth, oral mucositis, rampant dental caries, and radioactive osteomyelitis of jaw, which cause swallowing, chewing problems, and taste dysfunction. Currently, the research on radioactive salivatitis is progressing rapidly, but its mechanism is more complication. This paper review aims to summarize the research progress in this field.


Subject(s)
Carcinoma, Squamous Cell , Dental Caries , Head and Neck Neoplasms/radiotherapy , Humans , Mouth Neoplasms , Radiation Injuries , Salivary Glands , Xerostomia/etiology
17.
J Cancer Res Ther ; 2020 Jan; 15(6): 1464-1470
Article | IMSEAR | ID: sea-213556

ABSTRACT

Objective: The aim of this study is to develop a predictive model for identifying true negatives among nonspecific benign results on computed tomography-guided lung biopsy. Materials and Methods: This was a single-center retrospective study. Between December 2013 and May 2016, a total of 126 patients with nonspecific benign biopsy results were used as the training group to create a predictive model of true-negative findings. Between June 2016 and June 2017, additional 56 patients were used as the validation group to test the constructed model. Results: In the training group, a total of 126 lesions from 126 patients were biopsied. Biopsies from 106 patients were true negatives and 20 were false-negatives. Univariate and multivariate logistic regression analyses were identified a biopsy result of “chronic inflammation with fibroplasia” as a predictor of true-negative results (P = 0.013). Abnormal neuron-specific enolase (NSE) level (P = 0.012) and pneumothorax during the lung biopsy (P = 0.021) were identified as predictors of false-negative results. A predictive model was developed as follows: Risk score = −0.437 + 2.637 × NSE level + 1.687 × pneumothorax - 1.82 × biopsy result of “chronic inflammation with fibroplasia.” The area under the receiver operator characteristic (ROC) curve was 0.78 (P < 0.001). To maximize sensitivity and specificity, we selected a cutoff risk score of −0.029. When the model was used on the validation group, the area under the ROC curve was 0.766 (P = 0.005). Conclusions: Our predictive model showed good predictive ability for identifying true negatives among nonspecific benign lung biopsy results

18.
Article in Chinese | WPRIM | ID: wpr-873030

ABSTRACT

Objective:To improve the quality standard of Shenwei Gubi tablets, and to explore the reasons for the great difference in the contents of quality control index components between batches of this product. Method:The fingerprint of this product was established by HPLC, the determination was performed on Diamonsil C18 column (4.6 mm×250 mm, 5 μm) with acetonitrile (A)-0.1% phosphoric acid solution (B) for gradient elution (0-5 min, 10%A; 5-15 min, 10%-12%A; 15-30 min, 12%-26%A; 30-43 min, 26%-31%A, 43-50 min, 31%-40%A, 50-70 min, 40%-55%A; 70-84 min, 55%-72.5%A) as the mobile phase at detection wavelength of 230 nm. The orthogonal partial least squares-discriminant analysis-variable importance in the projection (OPLS-DA-VIP) map was drawn with the common peak as the independent variable. The contribution of 26 common peaks to the fingerprint differences among different batches of this product was quantified. By searching for the chromatographic peaks with great differences, combined with relevant literature, the components related to the clinical indications of the product were screened out and their contents were determined by specificity experiment, and the quantitative indicators were finally selected. HPLC-doide array detector (DAD) was employed to determine the contents of the above preferred indexes with detection wavelengths of 236, 276, 230, 322 nm, other conditions were the same as HPLC fingerprint detection method. Result:A total of 26 common peaks were calibrated on the HPLC fingerprint of Shenwei Gubi tablets. The similarity between the fingerprint of each batch samples and the reference fingerprint was≥0.950. Loganic acid, gentiopicroside, paeoniflorin and osthole were optimized as the quantitative indicators of this product, their average contents were 161.02, 401.80, 255.54, 80.68 μg·g-1. Conclusion:The established fingerprint and multi-index quantitative analysis method are stable and reliable, and can be used for quality control of Shenwei Gubi tablets. Difference in contents of quality control components between batches of raw materials and the imperfect quality control method of intermediates in the production process are the main reasons for the great difference in the contents of quality control indicators between batches of this product.

19.
Article in Chinese | WPRIM | ID: wpr-872165

ABSTRACT

Objective:To develop a simple, safe and effective surgical treatment of complextraumatic tattoos and to evaluate the long-term results.Methods:Eight patients with complextraumatic tattoos from October 2013 to June 2017, were chosen for this study. The operations were carried out under local tumescent anesthesia and the traumatic tattoos were removed with full-thickness skin by skin biopsy punches. The wound was covered with Shengji Yufu Ointment until healing.Results:All patients got good recover from the successful operations and no early complications occurred in this group. 8 patients were followed up one year postoperation and they all obtained ideal outcomes. No clear scar was notet in the skin.Conclusions:The wound self-healing treatment of complex traumatic tattoos with skin biopsy punches could obtain ideal outcomes and the scar is not obvious. The long term results prove that the surgical procedure in this paper has the advantages of less injury, simple, safe and effective. It provides a new way of thinking and method for treating complex traumatic tattoo in clinic, especially suitable for facial complex traumatic tattoo, and worth to clinical promotion.

20.
Article in Chinese | WPRIM | ID: wpr-871874

ABSTRACT

Objective:To investigate the relationship between apolipoprotein E (APOE) gene polymorphism and cerebral infraction (CI) in Chinese type 2 diabetes mellitus (T2DM) patients.Methods:This study included 245 samples of T2DM patients without cerebral infraction (CON group) (Male/Female, 128/117) and 270 samples of T2DM patients with cerebral infraction (CI group)(Male/Female, 145/125) from the department of endocrinology and neurology utilizing real-time fluorescence quantitative PCR technique. The t test and χ 2 test were used to compare the differences between the two groups. Results:Patients with a history of hypertension in the CI group (84.12%) were significantly higher than those in the CON group (70.42%) (χ 2=15.91, P<0.05).The systolic blood pressure (142.78±20.52)mmHg of the CI group was significantly higher than that of the CON group (133.89±18.58)mmHg ( t=-5.16, P<0.05).Compared with CON group, the frequency of genotypes of ε2/ε3 and ε3/ε4 in CI group was significantly higher, while the frequency of ε3/ε3 genotype was significantly lower (χ 2=11.48, P<0.05); the allele frequency of APOE ε4 was higher while ε3 was lower in CI group than that in CON group (χ 2=7.00, P<0.05). Logistic regression analysis showed that hypertension history ( OR=1.95, P<0.05), high systolic blood pressure ( OR=1.02, P<0.05), APOE genotypes of ε2/ε3 ( OR=2.08, P<0.05) and ε3/ε4 ( OR=1.85, P<0.05) were independent risk factors for cerebral infarction in T2DM patients. Conclusion:The polymorphism of APOE gene may be related to cerebral infraction in Chinese T2DM patients.

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