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1.
Article in Chinese | WPRIM | ID: wpr-928136

ABSTRACT

The fingerprint of Boenninghausenia albiflora var. albiflora was established by ultra performance liquid chromatography(UPLC), and the content of 12 active components including chlorogenic acid was determined. Multivariate statistical analysis was used to explore the indicator components of B. albiflora var. albiflora and a comprehensive evaluation system was created for the quality of B. albiflora var. albiflora. In this study, 33 batches of B. albiflora var. albiflora with different sources were collected and studied, and the UPLC fingerprint of B. albiflora var. albiflora was developed. There were 37 common peaks, of which 12 components were identified, and the content of these 12 components was measured. In combination of the common peaks and the content of chemical components, multivariate statistical analysis was performed, and the results showed that 6 components [daphnoretin, isoimperatorin, astragalin, imperatorin, neochlorogenic acid, and isoquercitrin(weight coefficient>0.1)] were selected as chemical markers for the quality of B. albiflora var. albiflora. Technique for order of preference by similarity to ideal solution(TOPSIS) analysis and chemometrics revealed that the quality of S32, S28 and S29 were superior, while that of S12, S7 and S16 were inferior. The quality evaluation method of B. albiflora var. albiflora constructed in this study was accurate and reliable, with simpleness and easiness to operate. It is suggested that the 6 above-mentioned active components could be used as indicator components for quality control of B. albiflora var. al-biflora. The samples were harvested during the flowering and fruiting period, which is from the beginning of July to the end of August.


Subject(s)
Chromatography, High Pressure Liquid/methods , Chromatography, Liquid , Drugs, Chinese Herbal/chemistry , Multivariate Analysis , Quality Control
2.
Chinese Medical Journal ; (24): 919-928, 2020.
Article in English | WPRIM | ID: wpr-827683

ABSTRACT

BACKGROUND@#Gastric cancer (GC) is one of the most globally prevalent cancers in the world. The pathogenesis of GC has not been fully elucidated, and there still lacks effective targeted therapeutics. The influence of altered kinesin superfamily protein 22 (KIF22) expression in GC progression is still unclearly. The aim of this study was to investigate the KIF22 effects on GC and related mechanisms.@*METHODS@#Gastric carcinoma tissues and matching non-cancerous tissues were collected from patients with GC who have accepted a radical gastrectomy in Lanzhou University Second Hospital from May 2013 to December 2014. The expression of KIF22 was examined in GC of 67 patients and 20 para-carcinoma tissues by immunochemical staining. The relationship between the expression of KIF22 and clinicopathologic characteristics was next investigated in the remaining 52 patients except for 15 patients who did not complete follow-up for 5 years. Cell viability was performed via 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) test and colony formation assay in the MGC-803 and BGC-823 GC cells. Cell scratch and trans-well invasion assay was performed to assess migration ability in the MGC-803 and BGC-823 GC cells. Gene set enrichment analysis (GSEA) pathway enrichment analysis was performed to explore the potential functions. Cell cycle was detected by flow cytometry. In addition, the two GC cell lines were used to elucidate the underlying mechanism of KIF22 in GC in vitro via assessing the effects on mitogen-activated protein kinase and extracellular regulated protein kinases (MAPK/ERK) signal transduction pathway-related expressions by Western blotting assays. The differences were compared by t tests, one-way analysis of variance, and Chi-squared tests.@*RESULTS@#The study showed that KIF22 was up-regulated in GC, and KIF22 high expression was significantly related to differentiation degree (χ = 12.842, P = 0.002) and poorly overall survivals. GSEA pathway enrichment analysis showed that KIF22 was correlated with the cell cycle. Silence of KIF22 decreased the ability of the proliferation and migration in gastric cells, induced G1/S phase cell cycle arrest via regulating the MAPK-ERK pathways.@*CONCLUSIONS@#KIF22 protein level was negatively correlated with prognosis. KIF22 knockdown might inhibit proliferation and metastasis of GC cells via the MAPK-ERK signaling pathway.

3.
Article in English | WPRIM | ID: wpr-690638

ABSTRACT

<p><b>OBJECTIVE</b>To explore whether the intake of dietary carotenoids could protect against skeletal fluorosis in Guizhou province in which coal-burning fluorosis is endemic.</p><p><b>METHODS</b>A case-control study of 196 patients with skeletal fluorosis and 196 age and gender-matched controls was conducted in Zhijin, Guizhou Province. Face-to-face interviews were conducted to assess habitual dietary intake using a 75-item food frequency questionnaire and various covariates with structured questionnaires. Urinary fluoride was measured using an ion-selective electrode method. The genotype of superoxide dismutase 2 (SOD2) rs11968525 was detected by TaqMan method.</p><p><b>RESULTS</b>We observed significant dose-dependent inverse associations of skeletal fluorosis with intake of β-carotene, lutein/zeaxanthin, lycopene, and total carotenoids (P-trend = 0.002 to 0.018), whereas α-carotene and β-cryptoxanthin intakes were not found to be related to skeletal fluorosis, after adjustment for potential confounders. The adjusted ORs and 95% CI of skeletal fluorosis for the highest versus lowest quartile were 0.30 (0.10, 0.86) for β-carotene, 0.23 (0.08, 0.66) for lycopene, 0.26 (0.10, 0.75) for lutein/zeaxanthin and 0.34 (0.14, 0.74) for total carotenoids (all P-trend < 0.05). Stratified analyses showed that the protective effects of lutein/zeaxanthin and total carotenoids on skeletal fluorosis were more evident for individuals with the AG+AA genotypes of SOD2 (rs11968525).</p><p><b>CONCLUSION</b>Increased intakes of β-carotene, lutein/zeaxanthin, lycopene, and total carotenoids are independently associated with a lower risk of coal-burning skeletal fluorosis. SOD2 (rs11968525) polymorphisms might modify the inverse associations between dietary carotenoids and skeletal fluorosis.</p>


Subject(s)
Bone Diseases, Metabolic , Genetics , Urine , Carotenoids , Case-Control Studies , China , Coal , Energy Intake , Environmental Exposure , Feeding Behavior , Female , Fluoride Poisoning , Genetics , Urine , Fluorides , Urine , Humans , Middle Aged , Polymorphism, Genetic , Superoxide Dismutase , Genetics , Surveys and Questionnaires
4.
Article in Chinese | WPRIM | ID: wpr-743375

ABSTRACT

Objective To investigate the relationship between platelet-to-lymphocyte ratio(PLR) and the prognosis of diabetes ketoacidosis (DKA).Methods 120 patients with DKA were divided into two groups:the DKA survived group (n=86) and the DKA died group (n=34).80 patients with type 2 diabetes (T2DM)were also enrolled in our study as the control group.Their clinical and biochemical parameters were determined.PLR was calculated by blood routine examination.Results Patients in the DKA died group had higher PLR than patients in the DKA survived group and the control group.According to logistic regression analysis,age [OR=2.119(95%CI 1.506-3.994),P=0.032],infection [OR=2.047(95% CI 1.421-3.885),P=0.021],Glasgow coma score [OR=0.523(95% CI 0.394-0.693),P=O.O11],simultaneous other organ dysfunction [OR=3.642 (95% CI 2.287-5.442),P=0.006],PLR[OR=3.073 (95% CI 1.951-5.142),P<0.001],mechanical ventilation [OR=3.846 (95% CI 2.623-5.113),P<0.001] and lactic acid [0R=2.854 (95% CI 1.514-4.216,P=0.008] were all significantly correlated with the prognosis of DKA.The optimal cutoff value of PLR for predicting the prognosis of DKA was 192.26.Its sensitivity and specificity were 82.40% and 67.60%,respectively.Conclusion PLR can be used as a sensitive indicator to predict the prognosis of DKA.

5.
Chinese Traditional Patent Medicine ; (12): 2045-2049, 2017.
Article in Chinese | WPRIM | ID: wpr-660582

ABSTRACT

AIM To optimize the aqueous extraction for polysaccharides from Astragali Radix and to evaluate the in vitro antitumor activity.METHODS With extraction temperature,extraction time and solid-liquid ratio as influencing factors,extraction rate of polysaccharides as an evaluation index,the extraction was optimized by uniform design.The effect of polysaccharides on the proliferation of non-small cell lung cancer NCI-H460 cells,the apoptosis rate and cell cycle of NCI-H460 cells,and the expressions of Caspase-3,Bax and Bcl-2 were detected by MTT assay,flow cytometry and Western blot,respectively.RESULTS The optimal conditions were determined to be 100 ℃ for extraction temperature,1 h for extraction time,and 1 ∶ 35 for solid-liquid ratio,the extraction rate of polysaccharides was 3.62%.Compared with the control group,the proliferation of NCI-H460 cells was significandy inhibited in a dose-dependent manner (P < 0.01),the S phase ratio,early apoptosis rate,late apoptosis rate and total apoptosis rate were markedly increased (P < 0.01),and the Caspase-3 expression and Bax/Bcl-2 ratio were also obviously increased (P < 0.01) in the polysaccharides group.CONCLUSION This fast,stable and reliable method can be used for the aqueous extraction for polysaccharides from Astragali Radix,which can significantly inhibit the proliferation of NCI-H460 cells and induce apoptosis of NCI-H460 cells.

6.
Chinese Traditional Patent Medicine ; (12): 2045-2049, 2017.
Article in Chinese | WPRIM | ID: wpr-657968

ABSTRACT

AIM To optimize the aqueous extraction for polysaccharides from Astragali Radix and to evaluate the in vitro antitumor activity.METHODS With extraction temperature,extraction time and solid-liquid ratio as influencing factors,extraction rate of polysaccharides as an evaluation index,the extraction was optimized by uniform design.The effect of polysaccharides on the proliferation of non-small cell lung cancer NCI-H460 cells,the apoptosis rate and cell cycle of NCI-H460 cells,and the expressions of Caspase-3,Bax and Bcl-2 were detected by MTT assay,flow cytometry and Western blot,respectively.RESULTS The optimal conditions were determined to be 100 ℃ for extraction temperature,1 h for extraction time,and 1 ∶ 35 for solid-liquid ratio,the extraction rate of polysaccharides was 3.62%.Compared with the control group,the proliferation of NCI-H460 cells was significandy inhibited in a dose-dependent manner (P < 0.01),the S phase ratio,early apoptosis rate,late apoptosis rate and total apoptosis rate were markedly increased (P < 0.01),and the Caspase-3 expression and Bax/Bcl-2 ratio were also obviously increased (P < 0.01) in the polysaccharides group.CONCLUSION This fast,stable and reliable method can be used for the aqueous extraction for polysaccharides from Astragali Radix,which can significantly inhibit the proliferation of NCI-H460 cells and induce apoptosis of NCI-H460 cells.

7.
Article in Chinese | WPRIM | ID: wpr-360172

ABSTRACT

Small intestinal hemangioma is a rare condition that can be divided histologically into capillary, cavernous or mixed types, among which the cavernous type is the most common. Here we report a case of small intestinal cavernous hemangioma with chronic hemorrhage in 44-year-old man. The patient complained of weakness and dizziness for 2 years that aggravated 1 month before admission accompanied by intermittent melena. Laboratory tests suggest severe anemia, and computed tomography, gastroscopy and colonoscopy all revealed signs of anemia. Capsule endoscopy detected small intestinal erosions, bleeding lesions and prominent neoplasms. An exploratory laparotomy was performed, in which the segment of the jejunum with lesions was resected. Pathological examination of the resected jejunum identified the neoplasm as cavernous hemangioma of the small intestine, which was the cause of severe anemia.

8.
Chinese Pharmaceutical Journal ; (24): 1379-1384, 2016.
Article in Chinese | WPRIM | ID: wpr-859004

ABSTRACT

OBJECTIVE: To study the effects of batanin on proliferation of HeLa cells and the relationships with pyruvate metabolism and glycolysis. METHODS: The HeLa cells were cultured in vitro and betanin at 0, 62.5 and 500 mg·L-1 was added after the attachment of the cells. The cells were harvested after 48 h of culture for the cell counting and storaged at low temperature. Referring to human genome, the frozen cells were carried out for the analysis and calculation of high-throughout transcrip-tome. Utilizing internet data base, the parts related with glycolysis, pyruvate metabolism and citric cycle were selected and analyzed to study the effect of betanin on the protein coding transcripts of the glycolysis and citric cycle in HeLa cells. RESULTS: Betanin with both of the 2 concentrations inhibited the proliferation of the cultured HeLa cells, and affected the transcript reads of the cultured cells. There were 74 of protein coding transcripts expressed in HeLa cells, but only 40 of them had the reads >1 or <1 but with significant difference between treatments. Betanin significantly up-regulated the protein coding transcripts of the phosphofructokinase (platelet) (ENST00000381075) and fructose-1,6-bisphosphatase 1 (ENST00000375326) which were rate-limited enzymes in the glycolysis and gluconeogenesis respectively, it was benefit for cell metabolism, possibly being the stress response of the cell to betanin. However, betanin significantly down-regulated the protein coding transcript of the mitochondial lactate dehydrigenase D (LDHD) (ENST00000450168) of HeLa cell, and the mitochondial phosphoenolpyruvate carboxykinase 2 (PEPCK) also had a tendency to being down-regulated, which all were related with pyruvate metabolism. CONCLUSION: The inhibition of betanin on proliferation of HeLa cell is related with the down-regulation of the protein coding transcripts of the LDHD and PEPCK of the mitochondrial pyruvate metabolism of the cell.

9.
Article in Chinese | WPRIM | ID: wpr-252541

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of emodin combined gemcitabine on the growth of pancreatic cancer in vivo and in vitro as well as its mechanisms.</p><p><b>METHODS</b>After human pancreatic cancer cell line SW1990 was treated with emodin (40 micromol/L), gemcitabine (20 micromol/L), and emodin combined gemcitabine, the cell proliferation was detected by cell counting kit-8 (CCK-8) assay. The apoptosis of pancreatic cancer cells was detected using the flow cytometry (FCM). The protein expressions of Bax and Bcl-2 were detected using Western blot. SW1990 cells were injected subcutaneously into nude mice to establish pancreatic xenograft tumors. The mice were then treated by emodin, gemcitabine, and emodin combined gemcitabine, respectively. The changes of tumor volume were monitored. The positive expressions of Ki-67, Bax, and Bcl-2 in the xenograft tumors were detected using immunohistochemical method.</p><p><b>RESULTS</b>Emodin combined with gemcitabine induced a higher percentage of growth inhibition and apoptosis in pancreatic cancer cell line SW1990 than that of gemcitabine or emodin alone (P < 0.05). The protein expression of Bax was up-regulated and that of Bcl-2 down-regulated in the emodin group and the emodin combined gemcitabine group when compared with the control group (P < 0.05). Emodin combined with gemcitabine could significantly inhibit the growth of pancreatic xenograft tumors, increase the positive expression of Bax in tumor tissues, obviously decrease the positive expressions of Ki-67 and Bcl-2 (P < 0.05). The optimal effects were obtained in the emodin combined gemcitabine group (P < 0.05).</p><p><b>CONCLUSION</b>Emodin could potentiate the inhibition of pancreatic cancer growth induced by gemcitabine both in vitro and in vivo, which might be achieved by up-regulating the expression of Bax and down-regulating the expression of Bcl-2.</p>


Subject(s)
Animals , Cell Line, Tumor , Cell Proliferation , Deoxycytidine , Pharmacology , Emodin , Pharmacology , Female , Gene Expression Regulation, Neoplastic , Humans , Ki-67 Antigen , Metabolism , Mice , Mice, Inbred BALB C , Mice, Nude , Pancreatic Neoplasms , Metabolism , Pathology , Proto-Oncogene Proteins c-bcl-2 , Metabolism , Xenograft Model Antitumor Assays , bcl-2-Associated X Protein , Metabolism
10.
Chinese Medical Journal ; (24): 2687-2691, 2011.
Article in English | WPRIM | ID: wpr-292822

ABSTRACT

<p><b>BACKGROUND</b>Primary open angle glaucoma (POAG) is a common cause of irreversible blindness. The variable etiology of POAG poses significant challenges for treatment and rehabilitation. We analyzed a large POAG patient cohort during treatment to reveal possible causes of vision disorder, assess vision-related quality of life (VRQL), and to evaluate the efficacy of rehabilitative treatments.</p><p><b>METHODS</b>We analyzed the visional disturbances in 500 POAG patients (890 eyes) by regular ophthalmic examination and visual field examination using Humphrey 30° perimetry. Appropriate rehabilitative treatments for POAG were prescribed based on results of clinical examination and included correction of ametropia, health education, counseling, and the fitting of typoscopes. VRQL was assessed before and after treatment by a VRQL self-assessment questionnaire.</p><p><b>RESULTS</b>Scores on the VRQL self-assessment were significantly lower compared to healthy controls. The primary cause of the vision disturbances was ametropia (97.99%), and 51.61% of the ametropia eyes had not received appropriate correction. The secondary causes of visual impairment were glaucomatous neurodegeneration (26.29%), complicated cataract, or other accompanying eye diseases. The causes of the clinical low vision (44 patients) were glaucomatous neurodegeneration (32 eyes), fundus diseases (23 eyes), keratopathy (11 eyes), and other eye diseases (10 eyes). The VRQL scores of patients improved significantly after rehabilitation and the correction of ametropia (P < 0.01). Twenty-five patients with low vision were provided with typoscopes, and 21 (84%) experienced significant functional recovery, while the remaining low vision patients could see letter lines two or more levels lower (smaller) on visual charts in a near vision test.</p><p><b>CONCLUSIONS</b>Vision disorders in POAG patients are common and severe. Appropriate rehabilitation, especially the correction of ametropia, can significantly improve VRQL as revealed by the self-assessment of POAG patients.</p>


Subject(s)
Adult , Aged , Female , Glaucoma, Open-Angle , Rehabilitation , Humans , Male , Middle Aged , Quality of Life , Surveys and Questionnaires , Vision Disorders , Rehabilitation
11.
Chinese Medical Journal ; (24): 2662-2665, 2010.
Article in English | WPRIM | ID: wpr-285768

ABSTRACT

<p><b>BACKGROUND</b>The blood supply to the eye comes from the retinal central vascular system of the ophthalmic artery and the ciliary vascular system. The ophthalmic artery stems from the ipsilateral internal carotid artery. If occlusion or stenosis occurs in the carotid artery, the blood perfusion to the ophthalmic artery becomes insufficient, leading to signs and symptoms of anterior and posterior ocular ischemia. The objective of this study was to evaluate the clinical characteristics and risk factors of ocular ischemic diseases caused by carotid artery stenosis.</p><p><b>METHODS</b>This study was a retrospective review of 145 patients with carotid artery stenosis. Fifty-eight patients who had symptoms of ocular ischemic disease caused by carotid artery stenosis formed group A and the other 87 patients who only had carotid artery stenosis formed group B. We analyzed the causes and course of disease, and relative risk factors, by comparing the two groups.</p><p><b>RESULTS</b>The degree of carotid artery stenosis in group A was higher than that in group B. And group A had a greater decrease of ophthalmic artery flow. Male, hypertension, hyperlipidemia, and smoking were significantly related to carotid artery stenosis. Amaurosis fugax was the most common ocular symptom in group A. The ocular ischemic diseases mainly included ischemic optic neuropathy, central/branch retinal artery occlusion, ophthalmoplegia externa, and ocular ischemic syndrome.</p><p><b>CONCLUSIONS</b>Carotid artery stenosis correlates with ocular ischemic diseases. Ophthalmologists must observe for ocular symptoms, which were the onset symptoms in some patients.</p>


Subject(s)
Adult , Aged , Aged, 80 and over , Carotid Stenosis , Eye Diseases , Female , Hemodynamics , Humans , Hyperlipidemias , Hypertension , Ischemia , Male , Middle Aged , Retrospective Studies , Risk Factors , Smoking
12.
Article in Chinese | WPRIM | ID: wpr-273828

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of Endostatin and SU6668 combined with 5-FU on the growth and metastasis of human colon cancer in vivo and its mechanism.</p><p><b>METHODS</b>Metastatic model of human colon cancer was established by orthotopic implantation of human tumor tissue into colon wall of nude mice. Twelve days later, mice were randomly divided into saline water control, Endostatin, SU6668, Endostatin plus SU6668, and Endostatin plus SU6668 and 5-FU groups, intraperitoneal injected respectively every day for four weeks. Six weeks after implication, the tumor weight, inhibition rates, intratumoral microvessel density (MVD) and metastasis were evaluated after the mice were sacrificed.</p><p><b>RESULTS</b>Compared with the control, tumor growth was significantly inhibited in mice treated respectively with Endostatin, SU6668, Endostatin plus SU6668 and Endostatin plus SU6668 and 5-FU with an inhibition rate of 0, 64.9%, 63.5%, 76.4% and 88.2% respectively,and MVD decreased significantly in treated groups [(18.10+/-5.65) vs (2.75+/-0.75), (3.17+/-0.58), (0.94+/-0.42) and (0.36+/-0.45)]. The incidences of peritoneal and region lymph node metastases were significantly inhibited in Endostatin, SU6668, Endostatin plus SU6668 and Endostatin plus SU6668 and 5-FU (90% vs 16.7%, 25%, 0 and 0; 90% vs 0, 0, 0 and 0). The growth and metastasis of human colon cancer implanted in nude mice were significantly inhibited in Endostatin, SU6668, Endostatin plus SU6668, and Endostatin plus SU6668 and 5-FU, and the effect of Endostatin plus SU6668 and 5-FU was the most obviously.</p><p><b>CONCLUSION</b>Endostatin plus SU6668 and 5-FU has strong inhibitory effect both on tumor growth and metastasis of human colon cancer.</p>


Subject(s)
Angiogenesis Inhibitors , Therapeutic Uses , Animals , Cell Line, Tumor , Colonic Neoplasms , Drug Therapy , Pathology , Endostatins , Therapeutic Uses , Fluorouracil , Therapeutic Uses , Humans , Indoles , Therapeutic Uses , Lymphatic Metastasis , Male , Mice , Mice, Inbred BALB C , Mice, Nude , Neoplasm Transplantation , Neovascularization, Pathologic , Pyrroles , Therapeutic Uses
13.
Article in Chinese | WPRIM | ID: wpr-669452

ABSTRACT

Aim To study the change of the expression of the gp80 and gp130 subunits of the IL- 6 receptor (IL- 6R)in the osteoporotic bone marrow, to explore the role of IL- 6 receptor system in the osteoporosis resulted from the loss of estrogen.Methods Twenty female 12- week old SD rats [weight (200± 20) g] were randomly divided into 2 groups: the osteoporosis model group (10 rats),the control group (10 rats).Respectively in the 0th ,12th week after ovariectomy to analyze the levels of gp80 and gp130.Results In the 0th,12th week after ovariectomy,the level of gp130 (pg/μ g total RNA): the control group's level had no significant change (80± 3 vs 82± 4,t=0.85,P >0.05), while the osteoporosis group's level became significant higher (80± 3 vs 290± 40,t=6.46,P< 0.01).In the 0th ,12th week after ovariectomy, the level of gp80(pg/μ g total RNA): the control group's level had no significant change (85.4± 2.7 vs 85.6± 2.8,t=0.35,P >0.05), while the osteoporosis group's level became significant higher (85.4± 2.7 vs 210± 40,t=6.32,P< 0.01).Conclusion There was a significant increase of the expressions of gp130 and gp80 in the bone marrow of osteoporotic rat.

14.
Article in Chinese | WPRIM | ID: wpr-347305

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of dentin phosphoprotein (DPP) in inducing dentinal mineralization.</p><p><b>METHODS</b>Human DPP was combined with EAH-Sepharose 4B beads and its function of inducing mineralization was studied in mineralization system in-vitro. The mineral formed on the surface of the beads was analyzed by scanning electron microscopy (SEM) and the structure was analyzed by X-ray diffraction and plasma emission spectrum.</p><p><b>RESULTS</b>There was mineral formed on the beads with combined DPP and the mineral was calcium phosphates whose ratio of calcium to phosphate was 1.33. The diffractogram of the formed mineral was more similar to hydroxyapatite than to other calcium phosphates.</p><p><b>CONCLUSION</b>When tightly combined with certain support substance, human DPP can induce mineralization.</p>


Subject(s)
Calcium Phosphates , Chemistry , Metabolism , Dentin , Chemistry , Metabolism , Dentinogenesis , Humans , Microscopy, Electron, Scanning , Minerals , Chemistry , Metabolism , Phosphoproteins , Chemistry , Metabolism , Tooth Calcification , X-Ray Diffraction
15.
Article in Chinese | WPRIM | ID: wpr-253773

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the relationship between dentine phosphoprotein (DPP) and remineralization of demineralized dentine.</p><p><b>METHODS</b>(1) Soluble DPP was extracted with 1 mol/L NaCl from demineralized dentine and was evaluated. (2) Soluble DPP was removed with 0.1 mol/L NaCl or was not removed from demineralized dentine sections in human tooth roots. Then all sections were subjected to remineralization treatment, and remineralization degrees were compared by atomic absorption spectrum, SEM and microradiography.</p><p><b>RESULTS</b>(1) Soluble DPP was extracted with 1 mol/L NaCl. (2) Removal of soluble DPP resulted in significantly lower calcium concentration in remineralization solution (P < 0.01), less mean light-absorbed value in demineralized dentin sections by microradiography (P < 0.01).</p><p><b>CONCLUSIONS</b>Soluble DPP may have an inhibiting effect on remineralization of demineralized dentine, this study suggests that the remove of soluble DPP from root caries lesions may enhance their remineralization potential.</p>


Subject(s)
Adolescent , Child , Dentin , Chemistry , Humans , In Vitro Techniques , Phosphoproteins , Physiology , Tooth Demineralization , Tooth Remineralization
16.
Article in Chinese | WPRIM | ID: wpr-538853

ABSTRACT

Gene expressions of interleukin-6 receptor subunit gp130 and gp80 were investigated by competitive RT-PCR in the rat model of osteoporosis. A significant increase in expressions of gp130 and gp80 was found in bone marrow of osteoporotic rat, this change may be a possible mechanism in osteoporosis due to loss of estrogen.

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