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Objective To explore the value of neuroendoscopy assisted keyhole microvascular decompression (MVD) for treatment of patients with hemifacial spasm (HFS).Methods The clinical data,operative data and follow-up results of 35 patients with HFS,admitted to our hospital from January 2007 to June 2011 and underwent neuroendoscopy-assisted keyhole MVD,were retrospectively analyzed.By suboccipital retrosigmoid approach,the 30° neuroendoscope was applied to perform multi-angle exploration of the entire nerve,distinguish the responsible vessels,and confrm the location of Teflon before and after placing the Teflon.Twenty-eight patients were performed MVD under microscope,and 7 cases were performed surgery under endoscope.Results In 35 patients with HFS,the effective rate was 97.1％,with 91.4％ (32/35) being completely cured and 5.7％ (2/35) being significantly relieved and one being unaffected.Follow-up was performed in these 34 successful cases for an average of 30 months (6-60 months).One patient recurred 16 months after operation with an recurrence rate of 2.9％.Six patients were determined the responsible vessels by endoscope.Correcting Teflon was executed in 5 patients after endoscope exploration.Concision Neuroendoscope can help avoid missing and distinguishing the responsible arteries accurately,assist to rectify the position of Teflon,enhance the effectiveness of MVD,and reduce the postoperative recurrence rate.
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Objective To explore the role of magnetic resonance tomographic angiography (MRTA) and neuroendoscope in determining the etiology of idiopathic trigeminal neuralgia. Methods Forty-nine patients with idiopathic trigeminal neuralgia, performed microvascular decompression were chosen; their clinical data, imaging data and operative data were retrospectively analyzed. All patients underwent preoperative MRTA; and before placing intraoperative Teflon, 30° neuroendoscope was applied for multi-angle exploration. Results Positive rate reached 77.6% under MRTA. All patients with positive results under MRTA were confirmed, without a false-positive. Neuroendoscope helped to determine whether the suspect vessels were the offending vessels in 4 patients. The total effective rate in 49 patients was 97.9%; pain disappeared in 47 patients (95.9%) and pain relieved in 1 (2.0%).Conclusion MRTA and neuroendoscope can help to determine the etiology of idiopathic trigeminal neuralgia, and improve the safety and effectiveness of MVD.
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To observe effect of E2F decoy DNA on proliferation and apoptosis of androgen-independent prostate cancer cell line PC-3M the binding specificity of the E2F decoy DNA to the PC-3M nuclear protein was detected by electrophoretic mobility shift assay (EMSA). E2F decoy DNA, ARE decoy DNA and Control decoy DNA were respectively transfected into PC-3M cells with lipofectamine.Their influence on cell proliferative activity was detected by MTT assay.The cell apoptotic rate was determined by flow cytometric(FCM) analysis and chromosome DNA ladder was detected by DNA gel electrophoresis.The change of mRNA expression of C-myc and CyclinD1 were detected by RT-PCR.The change of mRNA expression of C-myc and CyclinD1 were detected by Western-blot. EMSA demonstrated specific binding of the E2F decoy to E2F transcription factor.The PC-3M cell growth was inhibited after transfection. The apoptotic rate was 26.35 percent and DNA ladder could be observed after transfection.The expression of C-myc and CyclinD1 were inhibited. All these results indicated that E2F decoy DNA induced apoptosis of androgen-independent prostate cancer cell lines PC-3M and inhibited cell proliferation via inhibiting expression of C-myc and CyclinD1.
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<p><b>BACKGROUND</b>The expanded endonasal approach (EEA) is used sparingly by surgeons for resection of lesions in the ventrocranial base. Herein, we examined the anatomy of the ventrocranial base by endoscopy and comment on the use of EEA in clinical practice.</p><p><b>METHODS</b>Twenty artery-injected adult cadaveric heads were studied under surgical conditions using the endoscopic EEA. The extent of the surgical exposure, the endoscopic anatomic view and the maneuverability of surgical instruments about the suprasellar region were studied by the endoscopic EEA.</p><p><b>RESULTS</b>The EEA by endoscope can reach the suprasellar region. In this approach, the optocarotid recess, supra and infra-optic chiasm interspace, the ophthalmic artery and others were important anatomical landmarks for identification of the suprasellar region.</p><p><b>CONCLUSIONS</b>The endoscopic EEA can be used to remove many types of lesions in the ventrocranial base. The microanatomy observed using the endoscope provides important anatomical information on the suprasellar region for neurosurgeons.</p>