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1.
Article in Chinese | WPRIM | ID: wpr-463880

ABSTRACT

Objective To investigate the lentivirus-mediated RNA interference(RNAi) reduced human epidermal growth factor 2 (HER2) gene expression in ovarian cancer chemosensitivity.Methods Specifically targeted short hairpin RNA HER2 gene ( shRNA) lentiviral expression vector and negative control sequences lentiviral vector were constructed.stable expression of HER2-shRNA in SKOV3 cell line ( KD) and negative control cell line (NC) were established.The uninfected SKOV3cell lineserved as blank control(CON)group, sensitivity to changes in three kinds of cells to cisplatin chemotherapy in the HER2 gene expression was detected by RT-PCR and Western blot technique, CCK-8 assay CON and KD cells strains were established xenograft model in nude mice in each group to detect the expression of the HER2 protein, as well as by cisplatin chemotherapy tumor size. Results Expression results showed that a stable cell line HER2-shRNA and negative control cell lines had been successfully establish by fluorescence and flow cytometry.RT-PCR results showed KD cells relative HER2 mRNA expression levels compared with the NC group, the difference was statistically significant (P<0.01), whereas no significant difference when compared with the CON group, HER2 mRNA KD Group inhibition rate was (95.5 ± 0.12)%.After transduction specific HER2-shRNA ovarian cancer tumor cells, HER2 protein expression was significantly reduced, growth had slowed down significantly after cisplatin treatment ( P<0.01 ) .Conclusion Lentivirus-mediated HER2-shRNA can effectively inhibit the expression of the HER2 gene, increase chemosensitivity to cisplatin in ovarian cancer.

2.
Chinese Medical Journal ; (24): 2012-2015, 2014.
Article in English | WPRIM | ID: wpr-248056

ABSTRACT

<p><b>BACKGROUND</b>Breast cancer 2 (BRCA2) is an important breast cancer-susceptibility gene. Promoter polymorphisms in BRCA2 may affect its transcription and be associated with cancer prognosis.</p><p><b>METHODS</b>We identified five polymorphisms of the BRCA2 promoter region by in silico searching and direct sequencing: -254A/G (rs3092989), -908A/G (rs206117), -1134A/G (rs206115), -1144C/T (rs206116), and -1260CTTAGA/- (rs3072036). The -908A/G, -1134A/G, -1144C/T, and -1260CTTAGA/- polymorphisms were genotyped by direct sequencing in 491 breast cancer patients, and the -254A/G polymorphism was genotyped by Sequenom.</p><p><b>RESULTS</b>The -1144C/T polymorphism was associated with clinical outcome. Carriers of the TT genotype had longer disease-free intervals (DFIs, P = 0.029), especially among patients with sporadic unilateral breast cancer (P = 0.010). Linkage disequilibrium (LD) analysis showed that all the five single nucleotide polymorphisms (SNPs) were in LD (D' > 0.8). Carriers of haplotypes containing the -1144T allele showed longer DFIs (P = 0.049), and the result was more significant in patients with sporadic unilateral cancer (P = 0.018). There were no significant associations between the other polymorphisms and DFI.</p><p><b>CONCLUSIONS</b>The results of this study suggest that homozygosity for the BRCA2 T(-1144) allele is associated with a longer DFI in Chinese women with breast cancer. Further functional studies are warranted to clarify this relationship.</p>


Subject(s)
Adult , Aged , Aged, 80 and over , Asians , Genetics , Breast Neoplasms , Genetics , Pathology , Female , Genetic Predisposition to Disease , Genetics , Genotype , Haplotypes , Humans , Linkage Disequilibrium , Middle Aged , Polymorphism, Single Nucleotide , Genetics , Promoter Regions, Genetic , Genetics
3.
Article in Chinese | WPRIM | ID: wpr-390361

ABSTRACT

Objectlve To investigate the value of basic and contrast-enhanced ultrasound in the diagnosis of superficial cervical lymph nodes.Methods Five hundred and forty-five cases of superficial cervical lymph nodes were sacned by basic ultrasound,in which 52 cases were also scaned by contrast-enhanced ultrasound.All cases were performed ultrasound-guided biopsy.Lymph nodes were divided into benign group and malignant group according to pathology reports.The differences of the two groups were analysed,and statistical analysis was performed.Results Two hundred and thirty cases were benign,315 cases were malignant.S/L(P<0.01),RI(P<0.01),vascular pattern(P<0.01)and contrast enhancement pattern(P<0.01)between benign and malignant group showed statistical significant differences,while no statistical difference in coefficient correlation of the time-intensity curve between the two groups was found.Conclusions A combination of basic and contrastenhanced ultrasound can significantly enhance the ability to identify malignant lymph nodes from benign lymph nodes.

4.
Clinical Medicine of China ; (12): 459-462, 2009.
Article in Chinese | WPRIM | ID: wpr-395099

ABSTRACT

Objective To study the effects of N-acetylcysteine (NAC) on the proliferation, apoptosis and collagen synthesis of human lung fibroblast (HLF). Methods HLF was primarily cultured in complete medium of DMEM/F12. Different concentrations of NAC (5,10,20,40mmol/L) were administrated. Cell proliferations were tested by methylthiazolyltetrazolium (MTT) ,apoptosis and cell cycle were detected with Flow cytometer and mRNA and 40 mmoi/L )after 24 hours,the apoptosis rates were (34.38±5.80)%, (37.72±3.10)%, (44.05± 4.52) % and (59.18±5.24) % ) respectively, significantly higher than that of the controls (3.92±1.24) % pression of type Ⅰ procollagen in HLF was decreased significantly after administration of NAC at 5, 10,20, 40 mmol/L respectively (P < 0.01 ). Conclusion Administration of NAC induces apoptosis and directly inhibites the proliferation and the collagen synthesis of HLF.

5.
Tumor ; (12): 140-141, 2001.
Article in Chinese | WPRIM | ID: wpr-433851

ABSTRACT

Objective To study the effects of super-selected internal iliac artery incubation in the treatment of advanced gynecological malignant tumors. Methods 36 cases of advanced gynecological malignant tumors (16 ovarian carcinoma, 13 cervical carcinoma, 3 vaginal carcinoma, 2 endometrial carcinoma, 1 choriocarcinoma and 1 leiomyosarcoma of uterus) were treated with this method for 48 times. 31 of these 48 times were embolized in bilateral iliac arteries, 13,2 and 1 cases the inferior mesenteric artery, the hepatic proper artery and the ovarian artery were embolized respectively. Results 4 cases showed complete response, 23 showed partial response, no change in 5 cases and in the rest 4 cases the disease was still in progress. The main side effects were slight gastro-intestinal reactions and embolism symptoms but no incubation complication occurred. Conclusion The super-selected artery of incubation will give high local density of chemotherapeutic drugs and low concentration in general circulation. The effects are better in cervical, endometrial and vaginal carcinomas than in ovarian carcinoma.

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