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1.
Journal of Clinical Hepatology ; (12): 553-557, 2022.
Article in Chinese | WPRIM | ID: wpr-922952

ABSTRACT

Objective To investigate the value of international standardized ratio-to-platelet ratio (INPR) versus aspartate aminotransferase-to-platelet ratio index (APRI) and fibrosis-4 (FIB-4) in the diagnosis of liver fibrosis in patients with primary cholangitis (PBC). Methods A retrospective analysis was performed for the patients who underwent liver biopsy and were diagnosed with PBC in The First Affiliated Hospital of Zhengzhou University from October 2013 to March 2021. Scheuer score was used to systematically evaluate the degree of liver fibrosis (S0-S4 stage). According to the results of liver biopsy, the degree of liver fibrosis was classified as significant liver fibrosis (≥S2), progressive liver fibrosis (≥S3), and liver cirrhosis (S4). Related data including general information, liver function, routine blood test results, and blood coagulation were collected, and related formulas were used to calculate the values of the noninvasive serological models INPR, APRI, and FIB-4. The Kruskal-Wallis H test was used for comparison of continuous data between multiple groups, and the chi-square test was used for comparison of categorical data between multiple groups. A Spearman correlation analysis was used to evaluate the correlation between noninvasive models and liver fibrosis stage. The receiver operating characteristic (ROC) curve was used to evaluate the efficacy of the noninvasive serological models in the diagnosis of liver fibrosis degree, and the DeLong method was used for comparison of the area under the ROC curve (AUC). Results A total of 143 patients with PBC were enrolled in the study, among whom 4 had stage S0 liver fibrosis, 50 had stage S1 liver fibrosis, 46 had stage S2 liver fibrosis, 26 had stage S3 liver fibrosis, and 17 had stage S4 liver fibrosis. There was a significant difference in INPR value between the PBC patients with different liver fibrosis degrees ( χ 2 =27.347, P 0.05). Conclusion INPR is a simple and accurate noninvasive model for the evaluation of liver fibrosis and has a certain value in the diagnosis of liver fibrosis in PBC.

2.
Article in Chinese | WPRIM | ID: wpr-943106

ABSTRACT

Psoriasis is a common clinical chronic inflammatory skin disease with a complex and diverse etiology and unrevealed pathogenesis. In traditional Chinese medicine (TCM), psoriasis is caused by internal and external factors. To be specific, external factors such as external wind, cold, dampness, heat, insects, and other pathogenic factors can result in Qi obstruction, blood stasis, and loss of nourishment in the skin, and internal and external factors such as wind, dampness, and toxic qi attacking the exterior, heat and dryness in the blood aspect, difficulty in flourishing due to blood dryness, and blood deficiency in the body, combined with external contraction of wind and dryness trigger the disease. Modern doctors have conducted research from the blood aspect, including blood heat, blood deficiency, blood stasis, and blood dryness. Modern medicine believes that it is related to genetics, immunity, infection, and other factors, and the research on its mechanism focuses on genetic susceptibility, immune system disorder, bacterial infection, and other aspects. At present, various clinical therapies are available, mainly including systematic treatment and local external application of drugs. While treating psoriasis, TCM mainly employs oral administration or external application of Chinese medicine and traditional therapies to regulate the immune system and gene targets and resist oxidation, with high safety and few adverse reactions. At present, although the research on the mechanism of TCM in the treatment of psoriasis has been gradually deepened, there are few detailed summaries on the mechanism of TCM in the prevention and treatment of psoriasis. Based on the research on TCM and western medicine in the treatment of psoriasis, this paper reviewed the mechanism of TCM in the prevention and treatment of psoriasis and proposed a comprehensive clinical and experimental research profile, aiming to provide references for further exploring the pathogenesis, treatment, and corresponding mechanism of psoriasis.

3.
Article in Chinese | WPRIM | ID: wpr-884534

ABSTRACT

Objective:To compare the dose distribution among CyberKnife, Tomotherapy, Edge, Triology and γ-knife in stereotactic body radiation therapy (SBRT) for pancreatic cancer.Methods:Clinical data of 10 panreatic cancer patients receiving CyberKinife treatment were retrospectively analyzed. The treatment plans were designed by five apparatuses from five centers according to the uniform requirement. All plans were transferred to MIM system for the extraction of parameters, which mainly included D min, D mean and D max of PTV, conformity index (CI), new conformity index (nCI), homogeneity index (HI), gradient index (GI), coverage, D max and dose-volume of the stomach and bowel. Results:The best CI and nCI were obtained in Triology ( P<0.001), and the worst HI was found in γ-knife ( P<0.001). The best GI was found in CyberKnife, followed by γ-knife and Tomotherapy, and Edge showed the worst GI ( P<0.001). The highest D min of PTV was found in both Edge and Triology, while lower D min of PTV was found in CyberKnife and Tomotherapy ( P<0.001). Additionally, γ-knife provided the highest D mean and D max of PTV ( P<0.001). Regarding the organs at risk, the lowest D max and D 5cm 3 of the bowel ( P<0.001), D max of the stomach ( P=0.003), D max( P=0.001), D 5cm 3 ( P<0.001) and D 10cm 3 ( P=0.005) of the duodenum, D max( P<0.001) and D 0.35cm 3 ( P<0.001) of the spinal cord were found in CyberKnife. The highest D max of the bowel was found in γ-knife. Furthermore, the highest D 5cm 3 of the duodenum was demonstrated in Edge ( P<0.001) and Tomotherapy provided the highest D max( P<0.001) and D 0.35cm 3 of the spinal cord ( P<0.001). Conclusions:All five radiotherapy apparatuses can meet the requirement of SBRT for pancreatic cancer. More rapid dose fall-off could be obtained via CyberKnife and γ-knife. Triology and Edge provide better target conformity. CyberKnife can better protect the gastrointestinal tract.

4.
Article in Chinese | WPRIM | ID: wpr-910377

ABSTRACT

Objective:To preliminarily explore the effects of tumor treating fields (TTF) arrays on the dose distribution in the treatment of Glioblastoma (GBM) using combined radiotherapy and concurrent TTF.Methods:EDR2 and MatriXX plate ionization chamber were employed to measure the absorbed doses of tissues at different depths (< 1 mm, 3 mm, 5 mm, 1 cm, 1.5 cm, 3 cm, 5 cm, 10 cm, and 15 cm) in the case that TTF arrays and latex-free foam were attached and not attached on the surface. Then the absorbed doses were calculated, compared, and analyzed. For the volumetric arc therapy (VMAT) of 10 GBM patients, deep dose verification was performed using the Sun Nuclear ArcCheck 3D dose verification system and the D99%, Dmean, and D1% of tumors and OARs were assessed. Results:The surface dose increased by 173% in the case that TTF arrays and latex-free foam were attached to the surface compared with the case of the surface with nothing attached. The surface dose increased by 61.7% due to the attachment of low-density latex-free foam. The dose deviation gradually decreased with an increase in the depth and stabilized (about 4%) at a depth of greater than 1.5 cm. As indicated by the VMAT verification result, the D99%, Dmean, and D1% of PTV and CTV decreased by 1.1%-1.2% and the Dmean and D1% of OARs (i.e., brainstem, pituitary gland, optic chiasma, optic nerve, eyeball, and eye crystal) decreased by 0.7%-1.5% in the case that TTF array and latex-free foam were attached on the surface compared with the case the surface with nothing attached. Conclusions:The combined radiotherapy and concurrent TTF in the GBM treatment will lead to a slight reduction of the absorbed doses of targets and OARs but a significant increase in the absorbed doses of the scalp. Therefore, it is recommended that the scalp doses should be reduced as far as possible in the design of the radiation treatment plan to reduce the adverse reactions on the scalp of GBM patients.

5.
Article in Chinese | WPRIM | ID: wpr-910334

ABSTRACT

Objective:To study the effects of radiotherapy and the prognostic factors in hepatocellular cancer (HCC) patients with cardiophrenic angle or superior diaphragmatic lymph nodes metastasis (LNM).Methods:We retrospectively analyzed 56 HCC patients with cardiophrenic angle or superior diaphragmatic LNM who were treated with or without external beam radiation therapy (EBRT) in Zhongshan Hospital of Fudan University from Jan 2010 to Aug 2020. Patients were divided into two groups according to whether they received radiotherapy, EBRT group and non-EBRT group, and each group had 28 patients. Radiation fields included or excluded primary tumor in EBRT group, and the cardiophrenic angle or superior diaphragmatic LNM did not receive any local treatment in non-EBRT group. The response rate, survival rate, local control rate, prognostic risk factors of the two groups were studied.Results:After EBRT, the partial response rate and complete response rate were 32.1%(9/28) and 32.1%(9/28). The median survival rate of EBRT group was 16.1 months (95% CI 9.00-23.21, RR=3.63) vs. 6.9 months (95% CI 4.63-8.77, RR=1.06) for the non-EBRT group, with statistically significant difference ( χ2=15.53, P<0.05). Cardiophrenic angle or superior diaphragmatic lymph nodes 1-year local control rate for EBRT group and non-EBRT group were 37.0% vs. 10.7%, with statistically significant difference ( χ2=5.28, P<0.05). Since diagnosis of cardiophrenic angle or superior diaphragmatic LNM, 4 patients (14.3%) in the EBRT group vs. 13 patients (46.4%) in the non-EBRT group had higher alpha-fetoprotein (AFP) level after 3 months compared with the AFP before EBRT ( χ2=6.84, P<0.05). Multivariate analysis showed that multiple intrahepatic tumors, maximal diameter of intrahepatic tumors >5 cm, AFP≥400 μg/L, no EBRT were poor prognostic factors. Conclusions:EBRT can prolong overall survival and improve the control rate of lymph node of HCC patients with cardiophrenic angle or superior diaphragmatic LNM. Patients with multiple intrahepatic tumors, maximal diameter of intrahepatic tumors >5 cm, AFP≥400 μg/L and no EBRT have poor prognosis.

6.
Acta Pharmaceutica Sinica B ; (6): 1246-1260, 2021.
Article in English | WPRIM | ID: wpr-881196

ABSTRACT

Sulforaphane (SFN), a natural anti-tumor compound from cruciferous vegetables, has been reported to induce protective autophagy to cancer cells, which might impair the anti-tumor efficiency of SFN. However, the accurate function and mechanism of SFN inducing autophagy in cancers are still obscure, especially in esophageal squamous cell carcinoma (ESCC), one of malignancies with high incidence in North China. Here, we mainly explored the potential function of autophagy upon SFN treatment in ESCC and molecular mechanism. We demonstrated that SFN could inhibit cell proliferation and induce apoptosis by activating caspase pathway. Moreover, we found activation of NRF2 pathway by SFN was responsible for the induction of autophagy and also a disadvantage element to the anti-tumor effects of SFN on ESCC, indicating that SFN might induce protective autophagy in ESCC. We, therefore, investigated effects of autophagy inhibition on sensitivity of ESCC cells to SFN and found that chloroquine (CQ) could neutralize the activation of SFN on NRF2 and enhance the activation of SFN on caspase pathway, thus improved the anti-tumor efficiency of SFN on ESCC

7.
Article in Chinese | WPRIM | ID: wpr-863120

ABSTRACT

Objective:To investigate the risk factors for 90 d death after endovascular mechanical thrombectomy (MT) in patients with acute anterior circulation large-artery occlusive stroke.Methods:From October 2015 to March 2018, patients with acute anterior circulation large-artery occlusive stroke treated with MT in People's Hospital of Shanghai Pudong New Area and the Second Affiliated Hospital of Soochow University were enrolled retrospectively. The primary outcome events were defined as death within 90 d after operation. Univariate and multivariate logistic regression models were used to identify the independent risk factors for death within 90 d after operation. Results:A total of 116 patients were enrolled, 23 (19.8%) of them died within 90 d after operation. Univariate analysis showed that there were significant differences in age, baseline National Institutes of Health Stroke Scale (NIHSS) score, the Alberta Stroke Program Early CT Score (ASPECTS), and the proportion of the baseline NIHSS score classification (≤8, 9-15, ≥16), ASPECTS ≤7, the number of attempts to pass >3 times, modified Thrombolysis in Cerebral Infarction (mTICI) blood flow grade 2b/3, hemorrhagic transformation (HT), and symptomatic HT in the death group compared with the survival group (all P<0.05). Multivariate analysis showed that after adjusting for age, fasting blood glucose, baseline NIHSS score, number of attempts to pass >3, and mTICI grade 2b/3, lower ASPECTS (odds ratio [ OR] 0.647, 95% confidence interval [ CI] 0.456-0.917; P=0.014), longer time from onset to vascular recanalization ( OR 1.004, 95% CI 1.000-1.007; P=0.046) and symptomatic HT ( OR 13.522, 95% CI 2.719-67.258; P=0.001) were the independent predictors of death within 90 d. Conclusion:The ASPECTS, time from onset to recanalization, and symptomatic HT were the independent risk factors for death within 90 d after MT in patients with acute anterior circulation large-artery occlusive stroke.

8.
Article | WPRIM | ID: wpr-835622

ABSTRACT

Ulcerative colitis (UC) is a chronic inflammatory disease of the colon, with a steadily rising prevalence in Western and newly industrialized countries. UC patients have a cancer incidence as high as 10% after 20 years of the disease. Although the importance of fruits and vegetables in defense against UC is beginning to be appreciated, the mechanisms remain largely unclear. In the current study, we reported that dietary black raspberries (BRBs) decreased colonic inflammation in the mucosa and submucosa of interleukin (IL)-10 knockout (KO) mice. We then used colon, spleen, and plasma from those mice to investigate whether BRBs exert their anti-inflammatory effects by correcting dysregulated toll-like receptor (TLR)-4 signaling to downregulate prostaglandin E2 (PGE2). Other studies reported that spleen is the reservoir of macrophages and depletion of macrophages in IL-10 KO mice prevents the development of colitis. Our results showed that BRBs decreased the percentages of macrophages in spleens of IL-10 KO mice. Moreover, mechanistically, the BRB diet corrected dysregulated TLR-4 signaling in cells from the colon and spleen, decreased PGE2 and prostaglandin I2, and increased 15-lipoxygenase and its product, 13-S-hydroxyoctadecadienoic acid, in plasma of IL- 10 KO mice. Therefore, we have elucidated one of the anti-inflammatory mechanisms of BRBs, and have identified biomarkers that could be indicators of response in UC patients treated with them. Our findings with BRBs could well apply to many other commonly consumed fruits and vegetables.

9.
Acta Pharmaceutica Sinica B ; (6): 1004-1019, 2020.
Article in English | WPRIM | ID: wpr-828828

ABSTRACT

Dysregulation of mTORC1/mTORC2 pathway is observed in many cancers and mTORC1 inhibitors have been used clinically in many tumor types; however, the mechanism of mTORC2 in tumorigenesis is still obscure. Here, we mainly explored the potential role of mTORC2 in esophageal squamous cell carcinoma (ESCC) and its effects on the sensitivity of cells to mTOR inhibitors. We demonstrated that RICTOR, the key factor of mTORC2, and p-AKT (Ser473) were excessively activated in ESCC and their overexpression is related to lymph node metastasis and the tumor-node-metastasis (TNM) phase of ESCC patients. Furthermore, we found that mTORC1/ mTORC2 inhibitor PP242 exhibited more efficacious anti-proliferative effect on ESCC cells than mTORC1 inhibitor RAD001 due to RAD001-triggered feedback activation of AKT signal. Another, we demonstrated that down-regulating expression of RICTOR in ECa109 and EC9706 cells inhibited proliferation and migration as well as induced cell cycle arrest and apoptosis. Noteworthy, knocking-down stably RICTOR significantly suppresses RAD001-induced feedback activation of AKT/PRAS40 signaling, and enhances inhibition efficacy of PP242 on the phosphorylation of AKT and PRAS40, thus potentiates the antitumor effect of RAD001 and PP242 both and . Our findings highlight that selective targeting mTORC2 could be a promising therapeutic strategy for future treatment of ESCC.

10.
Chongqing Medicine ; (36): 460-462,466, 2018.
Article in Chinese | WPRIM | ID: wpr-691812

ABSTRACT

Objective To investigate the diagnostic value of 1.5T magnetic resonance imaging(MRI) in the left ventricular structure and cardiac function of the patients with primary dilated cardiomyopathy(IDCM).Methods Sixty-seven patients with ID-CM(IDCM group) in this hospital from January to December 2016 were selected and contemporaneous 45 healthy volunteers served as the control group.The left ventricular structure parameters[end diastolic diameter(EDD),end systolic diameter(ESD),densifying thickness (C),trabecular thickness (NC),interventricular septal basement thickness (IVS),short axis shortening rate (△D),wall thickening rate(△T)],and cardiac function status[left ventricular end diastolic blood volume(EDV),end-systolic blood volume (ESV),ejection fraction(EF),stroke volume(SV) and cardiac output(CO)] were analyzed in the two groups.Results The levels of NC,NC/C,IVS,EDD,ESD,TDD,ESV and EDV in the IDCM group were higher than those in the control group,while C,C/IVS,△D,△T,MM,TSD,CO,EF and SV were smaller than those in the control group,except for C and IVS,the difference in the intergroup comparison was statistically significant(P<0.05).In the IDCM group,compared with the grade Ⅰ-Ⅱ,ESV and EDV in the grade Ⅲ and Ⅳ were cncreased,while SV,CO and EF decreased,the difference statistically significant(P<0.05).With the grade increase and ESV and EDV values were increased,SV,CO and EF were decreased,the difference had statistical significance (P<0.05).Conclusion MRI can early analyze the left ventricular structure parameters and cardiac function status in the patients with IDCM,and can pre-judge the heart function situation effectively and qualitatively.

11.
China Pharmacy ; (12): 176-179, 2018.
Article in Chinese | WPRIM | ID: wpr-704545

ABSTRACT

OBJECTIVE:To study the chemical constituents of Euonymus amygdalifolius.METHODS:Silica gel column chromatogram,gel column chromatogram,TLC and semi-preparative HPLC were adopted to isolate and purify ethanol extract from E.amygdalifolius.The structure of compounds was analyzed and identified according to physical and chemical properties,spectral data (mass spectrurn,hydrogen spectrum and carbon spectrum).RESULTS:Ten compounds were isolated from ethanol extract of aerial part of E.amygdalifolius,i.e.taraxerol (1),sophoradiol (2),taraxerone (3),sorghumol (4),heptaecanoic acid (5),octadecenoic acid (6),β-Sitosterol (7),daucosterol (8),epifriedelinol (9) and friedelin (10).CONCLUSIONS:Above compounds are all isolated from E.amygdalifolius for the first time;compounds 1,2,4,5,6 are isolated and obtained from Euonymus A.for the first time.The study lay a foundation for quality evaluation of E.amygdalifolius.

12.
Article in Chinese | WPRIM | ID: wpr-701932

ABSTRACT

Objective To analyze the early diagnosis ,treatment methods and effect of pulmonary embolism in Department of Traumatic Orthopedics .Methods From October 2014 to October 2016, 100 patients with pulmonary embolism in trauma department of orthopedics of Datong Coal Group Co three hospital were selected as the research subjects.Combined with the basic data of patients and clinical manifestations ,the early diagnosis and clinical features were summarized.The chronic respiratory questionnaire(CRQ) and quality of life assessment table(QOL) were used to evaluate the respiratory status and living quality of the patients before and after treatment .At the same time, according to the different treatment methods , the effect of simple anticoagulant or combined with thrombolytic was compared.Results Before treatment,the CRQ total score,QOL total score were (5.9 ±1.3) points,(65.78 ± 23.91)points,respectively,which after treatment were (3.1 ±0.7) points,(30.92 ±13.45) points,respectively,the differences were statistically significant ( t =11.931, 20.104, all P <0.05 ).In 100 patients, after treated by anticoagulation alone or combined with anticoagulation and thrombolytic therapy ,there were significantly effective in 54 cases,effective in 33 cases,invalid in 9 cases,and 4 cases died,the total effective rate was 87.00%.The total effective rate of anticoagulant therapy was 81.58%,which of anticoagulation combined with thrombolytic therapy was 90.32%,the difference was not statistically significant (χ2 =1.593,P>0.05).Conclusion In the perioperative period of Department of Traumatic Orthopedics , pulmonary embolism should be timely diagnosed , and reasonable treatment can improve the respiratory function and life quality of patients ,anticoagulation combined with thrombolytic therapy can improve the efficacy.

13.
Article in Chinese | WPRIM | ID: wpr-692720

ABSTRACT

Objective To explore the clinical diagnostic value of parathyroid hormone(PT H)in the diagno-sis of chronic renal failure(CRF).Methods 162 cases of CRF patients who were treated in the Sichuan Pro-vincial People′s Hospital from January 2014 to October 2015 were selected as the CRF group,meanwhile 164 non-CRF(NCRF)patients and 167 persons undergoing healthy physical examination were selected as the NCRF group and the healthy control group respectively.The PTH level was detected in 3 groups.According to the detection results the receiver operating characteristic(ROC)curve was drawn,and the diagnostic value of PTH for CRF was evaluate.Results The PTH level in the CRF group was significantly higher than that in the NCRF group and healthy control group,the difference was statistically significant(P<0.05);when the di-agnostic cut off value of PT H for CRF was 75.00 nmol/L,the area under the ROC curve,sensitivity,specifici-ty,positive likelihood ratio and Youden index were 0.92,80.20%,97.80%,36.45 and 78.00 respectively.Con-clusion PT H has a good diagnostic value to CRF.With the cut off value of PT H for diagnosing CRF as 75.0 nmol/L,it can improve the efficiency of clinical diagnosis.

14.
Chinese Journal of Digestion ; (12): 365-370, 2018.
Article in Chinese | WPRIM | ID: wpr-806682

ABSTRACT

Objective@#To analyze the expression and prognostic significance of esophageal squamous cell carcinoma associated long non-coding RNA-1 (ESCCAL-1) in esophageal squamous cell carcinoma (ESCC) tissues. @*Methods@#From August 2011 to May 2013, 73 patients with ESCC, who received radical resection in The First Affiliated Hospital of Zhengzhou University and Henan Cancer Hospital, were enrolled. The expressions of ESCCAL-1 in esophageal tumor tissues and corresponding adjacent non-tumor tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR). T test, chi square test and multivariate analysis were performed for statistical analysis. @*Results@#The expression of ESCCAL-1 was 28.03±9.37 in esophageal tumor tissues of patients with ESCC, which was higher than that in corresponding adjacent normal tissues (11.39±4.15), and the difference was statistically significant (t=2.964, P<0.01). However there were no statistically significant differences in the expressions of ESCCAL-1 among the patients with different age, gender, histological grade, classification of Union for International Cancer Control (UICC), T stage or lymph nodes metastasis (all P>0.05). The median disease-free survival (DFS) time and overall survival (OS) time of patients with low ESCCAL-1 expression were 39 months and 42 months, respectively, which were longer than those of patients with high ESCCAL-1 expression (30 months and 37 months), and the differences were statistically significant (χ2=9.049, P=0.003; χ2=10.165, P=0.001). The results of multivariate analysis showed that ESCCAL-1 expression was the independent risk factor of DFS and OS (high risk (HR)=2.45, 95% confidence interval (CI) 1.22 to 4.93, P=0.012; HR=2.29, 95%CI 1.14 to 4.59, P=0.019). @*Conclusions@#ESCCAL-1 may be involved the genesis and development of ESCC. The expression of ESCCAL-1 in esophageal tumor tissues may be a prognostic parameter for patients with ESCC receiving radical resection.

15.
Chinese Medical Ethics ; (6): 237-240, 2017.
Article in Chinese | WPRIM | ID: wpr-509525

ABSTRACT

Our country has made great achievements in organ transplantation,but there still exist many problems such as shortage of organs,seriously hindering the development of organ transplantation.Family factor,one of the influencing factors of organ transplantation in our country,has the characteristics of persistence and permanency.Researches on family factors will be helpful for the healthy development of organ transplantation.Started from family factors,this paper discussed the ethical issues of organ transplantation in our country and proposed appropriate measures such as changing moral cognition,advocating family support,strengthening community family intervention,correctly dealing with family ethics relationship,in order to provide a useful reference for the development of organ transplantation in China.

16.
Article in English | WPRIM | ID: wpr-20743

ABSTRACT

Acute myocardial infarction (AMI) remains a leading cause of morbidity and mortality worldwide. The exploration of new biomarkers with high sensitivity and specificity for early diagnosis of AMI therefore becomes one of the primary task. In the current study, we aim to detect whether there is any heart specific long noncoding RNA (lncRNA) releasing into the circulation during AMI, and explore its function in the neonatal rat cardiac myocytes injury induced by H2O2. Our results revealed that the cardiac-specific lncRNA MHRT (Myosin Heavy Chain Associated RNA Transcripts) was significantly elevated in the blood from AMI patients compared with the healthy control (*p<0.05). Using an in vitro neonatal rat cardiac myocytes injury model, we demonstrated that lncRNA MHRT was upregulated in the cardiac myocytes after treatment with hydrogen peroxide (H2O2) via real-time RT-PCR (qRT-PCR). Furthermore, we knockdowned the MHRT gene by siRNA to confirm its roles in the H2O2-induced cardiac cell apoptosis, and found that knockdown of MHRT led to significant more apoptotic cells than the non-target control (**p<0.01), indicating that the lncRNA MHRT is a protective factor for cardiomyocyte and the plasma concentration of MHRT may serve as a biomarker for myocardial infarction diagnosis in humans AMI.


Subject(s)
Animals , Apoptosis , Biomarkers , Diagnosis , Early Diagnosis , Heart , Humans , Hydrogen Peroxide , Mortality , Myocardial Infarction , Myocytes, Cardiac , Plasma , Rats , RNA , RNA, Long Noncoding , RNA, Small Interfering , Sensitivity and Specificity
17.
Military Medical Sciences ; (12): 937-940, 2016.
Article in Chinese | WPRIM | ID: wpr-508912

ABSTRACT

Objective To establish a numerical model of the shear module of alginate beads stimulated by ultrasonic sound.Methods The compression ratio and force of alginate beads were recorded with the compression test .The shear module of beads was measured with a Hertz model .When to alginate beads were stimulated ultrasonically for different durations , the ultrasonic stimulation power , ultrasonic pulse ratio , and changes in the shear module and solution temperature were measured.Results Temperatures in the solution and shear module of alginate beads increased under different ultrasonic stimulation conditions .Modeling analysis revealed the relationship between the shear module of alginate beads and the corresponding temperature .The shear module of beads was in a quadratic equation with temperature (20℃

18.
Article in Chinese | WPRIM | ID: wpr-606472

ABSTRACT

Purpose To assess the clinical and pathological features of small gastrointestinal stromal tumours (sGIST).Methods To reevaluated the clinical,histological and immunohistochemical parameters of 21 sGISTs.The standard immunohistochemical panel antibodies were studied on the tumor sections.All data were compared with clinical sGIST.Results There were a total of 7 females and 14 males of sGISTs.The median age was 63 years old.The tumors were predominantly located in the stomach showing a spindle cell morphology and the tumor sizes ranged from 0.5 cm to 1.5 cm.9 sGISTs combined with malignant tumors,which were gastric cancer have been incidentally detected during surgery.As the lesions were small in size,with infrequent bleeding,necrosis,mucosal invasion,ulceration and less mitotic index,sGISTs reoccurred less compared with clinical sGIST.p53,Ki-67 labeling index and microvascular density (MVD) in sGIST were significantly lower than clinical sGIST (P < 0.05).Conclusion sGIST may occure with digestive tract cancer synchronously.p53,Ki-67 labeling index and MVD were lower than clinical GIST,which means better prognosis.

19.
Article in Chinese | WPRIM | ID: wpr-605640

ABSTRACT

Objective To investigate the expression and clinical value of miRNA -22 in esophageal and gastric cancer tissues and cell lines.Methods Realtime -PCR was performed to detect the expression of miRNA -22 in human esophageal cancer cell lines ECA109,TE -1,TE -8,TE -13 and normal esophageal epithelial cells HEEC,48 cases of esophageal cancer tissues and matched adjacent normal tissues,human gastric cancer cell lines SGC -7901,MKN -45,NCI -N87,AGS,NUGC -3,SUN -1,normal human gastric mucosa cell line GES -1 and normal stomach fibroblastic cell NSFC,88 cases of gastric cancer tissues and matched adjacent normal tissues.Results The expression of miRNA -22 was much less in four esophageal cancer cell lines than that of immortalized normal esophageal epithelial cells HEEC,and the expression of miRNA -22 was much less in six gastric cancer cell lines than that of gastric mucosal epithelial cell line GES -1 and normal stomach fibroblastic cell line NSFC.The esophageal cancer tissues showed aberrant down regulation of miRNA -22 compared with the adjacent non -tumor tissues [(3.51 ±1.05)vs.(11.23 ±2.95),t =18.13,P <0.05].The gastric cancer tissues showed aberrant down regula-tion of miRNA -22 compared with the adjacent non -tumor tissues [(2.15 ±1.23)vs.(9.14 ±2.86),t =22.93, P <0.05].Conclusion The expression of miRNA -22 was much lower in esophageal and gastric cancer tissues and cell lines,which provided basement to nosogenesis,early diagnosis and create drug treatment of the cancers.

20.
Article in Chinese | WPRIM | ID: wpr-604449

ABSTRACT

Objective To investigate the expression and clinical value of miRNA -22 in gastric cancer tissues.Methods Real -time PCR was used to detect the miRNA -22 expression in gastric cancer tissues and matched adjacent tissues.The correlations of miRNA -22 expression with clinic pathological features were analyzed. Results 87.5%(77 /88)of the gastric tumor tissues showed aberrant down regulation of miRNA -22 compared with adjacent non -tumor tissues (χ2 =69.32,P 0.05 ). Conclusion The lower expression of miRNA -22 is associated with clinic pathological features.

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