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1.
Article in English | WPRIM | ID: wpr-928998

ABSTRACT

The clinical data for a patient with primary lung adenocarcinoma complicated with pulmonary hamartoma, who admitted to Zunyi Medical University Hospital in September 2020, was retrospectively analyzed. The 62-years-old male visited outpatient service because of dysphagia in March 2015, and the pulmonary nodules were found. In September 2020, the computed tomography indicated the enlarged nodule in the lower lobe of left lung with lobulation, and there was ground glass nodule in the upper lobe of left lung. After thoracoscopic wedge surgery, the primary pulmonary adenocarcinoma in the upper lobe of left lung and pulmonary hamartoma in the lower lobe of left lung were confirmed by pathology. Whole exon sequencing revealed that kinesin family member 20B (KIF20B) gene was not expressed in lung adenocarcinoma, but was expressed in pulmonary hamartoma. The clinical manifestations of lung adenocarcinoma complicated with pulmonary hamartoma was not typical, which could locate in the same side and different sides of the lung. The imaging manifestations of the 2 kinds of tumors were diverse and can not be completely distinguished. The pathological examination after surgery is the gold standard, and the possibility of malignant transformation of pulmonary hamartoma should be warned.


Subject(s)
Adenocarcinoma of Lung/complications , Hamartoma/surgery , Humans , Kinesins , Lung/pathology , Lung Neoplasms/surgery , Male , Middle Aged , Retrospective Studies
2.
Chinese Journal of Lung Cancer ; (12): 815-828, 2021.
Article in Chinese | WPRIM | ID: wpr-922244

ABSTRACT

Anaplastic lymphoma kinase (ALK) fusion gene, as a tumor driver gene, was crucial for the occurrence and development of non-small cell lung cancer (NSCLC). Recently, targeted ALK fusion gene has become the main treatment method for ALK-positive NSCLC. The first and second generation ALK inhibitors (ALKi), such as crizotinib, ceritinib, alectinib and ensartinib have been approved in China. However, there was no guidance for the management of ALKi adverse reactions. Therefore, this "Recommendations from experts in the management of adverse reactions to ALK inhibitors (2021 version)" has been summarized, led by Lung Cancer Professional Committee of Sichuan Cancer Society and Sichuan Medical Quality Control Center for Tumor Diseases, to provide practical and feasible strategies for clinical ALKi management specification of adverse reactions.
.


Subject(s)
Carcinoma, Non-Small-Cell Lung , Crizotinib , Humans , Lung Neoplasms/genetics , Protein Kinase Inhibitors/adverse effects , Receptor Protein-Tyrosine Kinases/antagonists & inhibitors
3.
Chinese Journal of Lung Cancer ; (12): 1049-1058, 2020.
Article in Chinese | WPRIM | ID: wpr-880222

ABSTRACT

BACKGROUND@#Pulmonary sclerosing pneumocytoma (PSP) is rare benign lung tumor which usually develops in middle-aged women without typical clinical and imaging findings. PSP consists of two basic cell types (surface cubic epithelial cells and round mesenchymal cells) and four histological types (hemorrhagic, sclerotic, solid and papillary). It grows slowly, but it can metastasize to distant organs. The pathology before surgery is easily misdiagnosed. This study aims to improve clinicians' understanding of PSP by discussing the clinical characteristics of the disease.@*METHODS@#This represents a retrospective study of thirty-five patients diagnosed with pulmonary sclerosing pneumocytoma by pathological examination from January 2011 to December 2019.@*RESULTS@#A total of 35 patients in this study, 12 cases were male and 23 cases were female, the average age is 51 years old. 7 cases were discovered accidentally by physical examination or routine chest computed tomography (CT), and 28 cases were found due to symptoms such as cough, sputum, hemoptysis and chest pain. The imaging changes is mainly featured with isolated or clear circular or round-like single nodule and lump in the lungs. In this group, 12 cases underwent percutaneous lung biopsy, only 7 cases were diagnosed with PSP. A total of 28 patients underwent surgery, 24 cases underwent rapid frozen pathological biopsy, only 5 cases diagnosed with PSP. Postoperative pathological examination results shows that 1 case was diagnosed with keratotic squamous cell carcinoma with partial PSP, and the rest were diagnosed with PSP. The surgical and non-surgical patients were followed up for 1 to 8 years after discharge, and the overall recovery was good. The patients were no recurrence and metastasis on chest CT review.@*CONCLUSIONS@#PSP is a clinically rare benign lung tumor, which is more common in middle-aged women. The clinical manifestations and imaging features are lack of significance. Percutaneous lung puncture pathological examination and intraoperative rapid frozen pathological sections often leads to misdiagnosis. Final diagnosis relies on postoperative pathological work-up for most cases.

4.
Chinese Journal of Lung Cancer ; (12): 798-804, 2019.
Article in Chinese | WPRIM | ID: wpr-781815

ABSTRACT

BACKGROUND@#Lung adenocarcinoma with miliary metastasis in both lungs is easily misdiagnosed. The aim of this study is to investigate the clinical features of lung adenocarcinoma with miliary metastases in both lungs and to improve the clinician's understanding of the disease.@*METHODS@#The clinical manifestation, radiology and pathology were analyzed in one patient with miliary intrapulmonary carcinomatosis in Affiliated Hospital of Zunyi Medical University. A review of literature was performed with "miliary intrapulmonary carcinomatosis", "lung cancer miliary", "pulmonary nodule, lung cancer" and "EGFR miliary" as key words in PubMed, Wangfang datebase and CNKI.@*RESULTS@#The patient was a 52 year-old woman with a history of productive cough for 2 months, which aggraveted with shortness of breath for 1 month. Her computed tomography of chest showed diffuse military nodules distributed at bilateral lungs. Computed tomography (CT)-guided needle biopsy of the left lung revealed lung adenocarcinoma and epidermal growth factor receptor (EGFR) exon 21 L858R mutation. The patient was treated with gefitinib 250 mg per day. The chest CT was reviewed several times during this period, which shows the double lung nodules were reduced. The patient is generally in good condition and her symptoms have improved. By literature review, we found relevant 7 Chinese articles and 56 English articles, a total of 16 cases have been reported. 17 patients were lung adenocarcinoma, 2 patients did not describe whether to detect EGFR gene mutations, 1 patient did not have EGFR gene mutation; 10 patients were EGFR exon 19 deletion, 1 patient was ALK positive, 1 patient was EGFR exon 21L858R mutation, 2 patients were EGFR exon 20 insertion.@*CONCLUSIONS@#Lung adenocarcinoma with miliary metastasis in both lungs is a rare phenomenon. We should pay attention to the performance to avoid misdiagnosis. Most of the adenocarcinoma subtypes have EGFR mutations, and EGFR-tyrosine kinase inhibitors (EGFR-TKIs) are the preferred treatment choice for this type of patients.

5.
International Journal of Surgery ; (12): 728-732,f3, 2019.
Article in Chinese | WPRIM | ID: wpr-801568

ABSTRACT

Objective@#By modeling ischemic foot ulcers in experimental rabbits, the mechanism of healing in animal models was investigated.@*Methods@#Sixty healthy male clean grade New Zealand rabbits were 3 months. The weight range was 2.1-2.5 kg. Randomly draw auording to the number, the experimental rabbits were randomly divided into 4 groups, namely, no ischemia-no ulcer-no intervention group (group A), no ischemia-no ulcer-no intervention group (group B), no ischemia-no ulcer-no intervention group (group C) and no ischemia-ulcer-intervention group (group D), with 15 rabbits in each group. Ischemic foot ulcers experimental rabbit building complete after 24 h, will naturally exposed ischemic foot ulcers in the low frequency electromagnetic field on experimental rabbit 7 d feeding experiment, the experimental rabbits ischemia crus muscle and the ulcer base were collected, and plasma by enzyme-linked immunosorbent assay (ELISA) verification tests in experimental rabbit plasma hypoxia-inducible factor 1 alpha subunit (HIF-1α), matrix metalloproteinases 9 (MMP-9) and soluble leukocyte differentiation antigen 40 ligand 1 (sCD40L1) expression level, while detecting ulcer healing area change, hematoxylin-eosin staining was used to detect and analyzed the pathological changes of ulcerated skin. Measurement data were expressed as mean±standard deviation (Mean±SD), one-way analysis of variance was used for comparison between groups, and LSD method was used for pairwise comparison.@*Results@#The concentration of MMP-9 and sCD40L1 in group D were (40.510±10.155) ng/ml and (44.580±19.138) ng/ml, respectively. Concentrations in group C were (93.210±17.838) ng/ml and (318.500±52.680) ng/ml, respectively. Group D was significantly lower than group C, and the difference was statistically significant (P< 0.000 1). The concentrations of HIF-1α in group D was (249.700±71.824) ng/ml, and that in group C was (124.830±20.110) ng/ml. The concentration in group D was significantly higher than that in group C, and the difference was statistically significant (P< 0.000 1). The concentrations of HIF-1α, MMP-9 and sCD40L1 in group B were (181.590±31.927), (78.950±16.652) and (173.670±43.048) ng/ml, respectively. The concentrations of group A were (35.420±9.916), (32.700±6.449) and (47.440±11.831) ng/ml, respectively. The concentration in group B was significantly higher than that in group A, the difference was statistically significant(P<0.000 1). The concentration of HIF-1α in group D was (249.700±71.824) ng/ml, and the concentration of group A was (35.420±9.916) ng/ml. The concentration of group D was significantly higher than that of group A, and the difference was statistically significant (P=0.000 1). The concentration of the MMP-9 in the group D was compared with the concentration of group A, and the difference was not statistically significant (P=0.207). The concentration of sCD40L1 in group D was (44.580±19.138) ng/ml, and that in group A was (47.440±11.831) ng/ml. The difference between group D and group A was not statistically significant(P=0.858). The healing area of ulcer was (1.855±0.394) cm2 in group D and (0.653±0.269) cm2 in group C. Group D was significantly higher than group C, and the difference was statistically significant (P<0.000 1). Hematoxylin-eosin staining results of ulcer skin showed that the epithelial of ischemic foot ulcer was significantly atrophied, hyperkeratinized, and the acinous cell layer was atrophied with a small amount of obvious inflammatory cell infiltration and hyperplasia of small vessels. After intervention, the symptoms were relieved.@*Conclusion@#Low frequency electromagnetic fields can promote the expression of HIF-1α in experimental rabbits, inhibit the expression of MMP-9 and sCD40L1, and promote the healing of ischemic foot ulcer.

6.
International Journal of Surgery ; (12): 728-732,封4, 2019.
Article in Chinese | WPRIM | ID: wpr-823517

ABSTRACT

Objective By modeling ischemic foot ulcers in experimental rabbits,the mechanism of healing in animal models was investigated.Methods Sixty healthy male clean grade New Zealand rabbits were 3 months.The weight range was 2.1-2.5 kg.Randomly draw auording to the number,the experimental rabbits were randomly divided into 4 groups,namely,no ischemia-no ulcer-no intervention group (group A),no ischemia-no ulcer-no intervention group (group B),no ischemia-no ulcer-no intervention group (group C) and no ischemia-ulcerintervention group (group D),with 15 rabbits in each group.Ischemic foot ulcers experimental rabbit building complete after 24 h,will naturally exposed ischemic foot ulcers in the low frequency electromagnetic field on experimental rabbit 7 d feeding experiment,the experimental rabbits ischemia crus muscle and the ulcer base were collected,and plasma by enzyme-linked immunosorbent assay (ELISA) verification tests in experimental rabbit plasma hypoxia-inducible factor 1 alpha subunit (HIF-1 α),matrix metalloproteinases 9 (MMP-9) and soluble leukocyte differentiation antigen 40 ligand 1 (sCD40L1) expression level,while detecting ulcer healing area change,hematoxylin-eosin staining was used to detect and analyzed the pathological changes of ulcerated skin.Measurement data were expressed as mean ± standard deviation (Mean ± SD),one-way analysis of variance was used for comparison between groups,and LSD method was used for pairwise comparison.Results The concentration of MMP-9 and sCD40L1 in group D were (40.510 ± 10.155) ng/ml and (44.580 ± 19.138) ng/ml,respectively.Concentrations in group C were (93.210 ± 17.838) ng/ml and (318.500 ± 52.680) ng/ml,respectively.Group D was significantly lower than group C,and the difference was statistically significant (P < 0.000 1).The concentrations of HIF-1α in group D was (249.700 ±71.824) ng/ml,and that in group C was (124.830 ±20.110) ng/ml.The concentration in group D was significantly higher than that in group C,and the difference was statistically significant (P < 0.000 1).The concentrations of HIF-1αt,MMP-9 and sCD40L1 in group B were (181.590 ± 31.927),(78.950 ± 16.652) and (173.670 ±43.048) ng/ml,respectively.The concentrations of group A were (35.420 ± 9.916),(32.700 ± 6.449) and (47.440± 11.831) ng/ml,respectively.The concentration in group B was significantly higher than that in group A,the difference was statistically significant (P < 0.000 1).The concentration of HIF-1 α in group D was (249.700 ± 71.824) ng/ml,and the concentration of group A was (35.420 ±9.916) ng/ml.The concentration of group D was significantly higher than that of group A,and the difference was statistically significant (P =0.000 1).The concentration of the MMP-9 in the group D was compared with the concentration of group A,and the difference was not statistically significant (P =0.207).The concentration of sCD40L1 in group D was (44.580 ± 19.138) ng/ml,and that in group A was (47.440 ± 11.831) ng/ml.The difference between group D and group A was not statistically significant(P =0.858).The healing area of ulcer was (1.855 ±0.394) cm2 in group D and (0.653 ± 0.269) cm2 in group C.Group D was significantly higher than group C,and the difference was statistically significant (P < 0.000 1).Hematoxylin-eosin staining results of ulcer skin showed that the epithelial of ischemic foot ulcer was significantly atrophied,hyperkeratinized,and the acinous cell layer was atrophied with a small amount of obvious inflammatory cell infiltration and hyperplasia of small vessels.After intervention,the symptoms were relieved.Conclusion Low frequency electromagnetic fields can promote the expression of HIF-1α in experimental rabbits,inhibit the expression of MMP-9 and sCD40L1,and promote the healing of ischemic foot ulcer.

7.
China Pharmacy ; (12): 2508-2512, 2019.
Article in Chinese | WPRIM | ID: wpr-817268

ABSTRACT

OBJECTIVE: To optimize the water extraction technology of total polysaccharide of Shuanghu capsules. METHODS: The total alkaloid was firstly extracted from Dendrobium nobile and Dendrobium officinale mixture of Shuanghu capsules with ethanol, and then total polysaccharide was extracted with water. Using glucose as control, total polysaccharide was treated with phenol-sulfuric acid method and its content was determined at 488 nm. Using comprehensive score calculated with the yield of the extract and the content of total polysaccharide as index, the effects of material-liquid ratio, extraction temperature, extraction time and times on the extraction were investigated by single factor test. Then L9(34) orthogonal test was used to optimize solid-liquid ratio,extraction temperature,extraction time and extraction times according to the results of single factor test. The optimized technology was validated. RESULTS: The linear range of glucose were 0.041 4-0.207 0 mg/mL(r=0.999 9). RSDs of intra-day and inter-day ranged 3.61%-8.24% (n=3,n=5), and RSD of repeatability test was 1.49% (n=6). Average recovery rate was 98.65%(RSD=1.45%,n=6). The optimal water extraction technology included solid-liquid ratio of 1 ∶ 25(g/mL),extraction temperature of 100 ℃,extracting for 90 min, extracting once. Results of validation tests showed that average content of total polysaccharide was 379.292 8 mg/g (RSD=1.93%,n=3) and average yield of the extract was 22.75%(RSD=2.41%,n=3). CONCLUSIONS: Established phenol-sulphuric acid method is simple, precise and accurate. The optimal water extraction technology is stable and feasible, which can be used for the extraction of total polysaccharides from Shuanghu capsules.

8.
China Pharmacy ; (12): 1909-1913, 2019.
Article in Chinese | WPRIM | ID: wpr-817204

ABSTRACT

OBJECTIVE: To optimize the ethanol extraction technology of total alkaloids from Shuanghu capsules. METHODS: Using dendrobine as control, the contents of total alkaloids from Dendrobium nobile and Dendrobium officinale in Shuanghu capsules were determined by acidic dyes colorimetry. Using comprehensive scores calculated by the yield of the extract and the contents of total alkaloids as evaluation indexes, the effects of soaking time, ethanol volume fraction, extraction time, solid-liquid ratio and extraction times were investigated with single factor tests. L9(34) orthogonal test was used to optimize ethanol volume fraction, extraction time, solid-liquid ratio and extraction times according to the results of single factor test. The optimized technology was validated. RESULTS: The linear range of dendrobine were 4.16-14.56 μg/mL (r=0.999 2). RSDs of repeatability and precision tests were all lower than 5%. Average recovery tests were 93.01% (RSD=1.97%, n=6). The optimal ethanol extraction technology included soaking for 12 h, ethanol volume fraction of 70%, solid-liquid ratio of 1 ∶ 12 (g/mL), extracting for 28 min, extracting 3 times. Results of validation test showed that the average yield of extract was 12.80% (RSD=4.39%, n=3), and the content of alkaloids was 0.359 0 mg/g(RSD=0.66%, n=3). CONCLUSIONS: Established acidic dyes colorimetry is simple, precise and accurate, which can be used for the content determination of total alkaloids. The optimized ethanol extraction technology is stable and feasible, and can be used for the extraction of total alkaloids from Shuanghu capsules.

9.
Article in Chinese | WPRIM | ID: wpr-692745

ABSTRACT

Objective To evaluate Loop-mediated isothermal amplification(LAMP)method on detecting sputum specimens from patients with tuberculosis.Methods 75 smear-positive sputum specimens and 72 smear-negative sputum samples from patients with tuberculosis were collected from the hospital during De-cember 2016 to April 2017.Each sputum sample was detected by sputum smear microscopy,Roche solid cul-ture(L-J)and LAMP simultaneously.The positive rate of smear-positive,smear-negative,LAMP and L-J cul-ture were calculated and statistically analyzed.Results In the 75 samples of sputum smear positive samples, LAMP method detected all the samples for positive,L-J culture tested 64 for positive.The positive rate of LAMP was 100.0%(75/75),L-J culture was 85.3%(64/75),and showed significant differences between the L-J solid culture and LAM P assay(χ2= 9.09,P<0.05).Among the 72 smear-negative sputum samples,the positive rate of LAMP was significantly higher than that of negative sputum smear[31.9%(23/72)],which was significantly higher than that of L-J culture[16.7%(12/72)],and the difference was statistically signifi-cant(χ2=6.67,P<0.05).Conclusion The LAMP method has higher sensitivity and specificity for detection of M tb than the methods of sputum smear microscopy and L-J culture with the characteristics of rapid,simple and accurate,therefore,it could be used for clinical early detection of TB patients.

10.
Article in Chinese | WPRIM | ID: wpr-806034

ABSTRACT

Objective@#To observe the expression of influenza virus sialic acid receptors(SAa2, 3-gal and SAa2, 6-gal)and the influence of dexamethasone intervention to its expression in the airway of asthmatic mice.@*Methods@#Thirty BALB/c mice were randomly divided into normal group (group N, n=10), asthma group (group A, n=10) and dexamethasone intervention group (group D, n=10). Mice of group A were sensitized and challenged by ovalbumin (OVA) to build asthmatic model. Mice in group D were given dexamethasone (2 mg/kg i. p.) 30 minutes before challenging, other procedures were the same as those of group A. The levels of IL-5 in the brochoalveolar lavage fluid (BALF) of mice in each group were determined by ELISA, the pathological changes of lung tissues were observed by HE staining, the degree of mucus secretion in airways were observed by AB-PAS staining, the expression of Mucin5ac (Muc5ac) in lung tissues were observed by immunohistochemical (IHC) staining, the expression of virus sialic acid receptors were observed by immunofluorescence.@*Results@#The counts of eosinophils, the level of IL-5 in BALF, the airway inflammatory pathological scores, the degree of mucus secretion, the expression of Muc5ac protein of group A were higher than those in group N and D (P<0.05). Receptors of SA α 2, 3-gal and SA α 2, 6-gal were expressed in all groups (P<0.05). The average positive rate for SA α 2, 3-gal receptor in group A were higher than that in group N. The average positive rate for SA α 2, 3-gal and SA α 2, 6-gal receptor in group D were higher than those of group N and A (P<0.05).@*Conclusion@#All these results indicate that the high expression of influenza virus sialic acid receptors due to asthma and dexamethasone intervention may be the mechanism of the enhanced susceptibility to influenza virus of asthma patients.

11.
China Pharmacy ; (12): 1655-1659, 2017.
Article in Chinese | WPRIM | ID: wpr-514041

ABSTRACT

OBJECTIVE:To evaluate the effectiveness and safety of inhaled furosemide in the adjuvant treatment of bronchial asthma systematically,and to provide evidenced-base reference for clinical treatment. METHODS:Retrieved from PubMed,Co-chrane library,CJFD,Wanfang database and VIP,randomized controlled trials(RCTs)about routine treatment+inhaled furosemide (trial group)vs. routine treatment(control group)in the treatment of bronchial asthma were collected. After quality evaluation and data extraction according to Cochrane systematic evaluation method 5.1.0,Meta-analysis was performed with Rev Man 5.3 soft-ware. RESULTS:A total of 13 RCTs were included,involving 708 patients. The results of Meta-analysis showed that:obvious ef-fective rate [RR=1.53,95%CI(1.28,1.84),P<0.001],total effective rate [RR=1.34,95%CI(1.23,1.45),P<0.001],the inci-dence of laryngeal discomfort [RR=10.79,95%CI(1.47,78.99),P=0.02] and gastrointestinal adverse reaction [RR=10.80,95%CI (1.43,81.53),P=0.02] in trial group were all significantly higher than control group,with statistical significance. There was no sta-tistical significance in the incidence of dry mount [RR=3.71,95%CI(0.81,16.86),P=0.09] and electrolyte disorder [RR=2.38, 95%CI(0.10,56.53),P=0.59] between 2 groups. CONCLUSIONS:Based on conventional treatment,the inhaled furosemide has good therapeutic efficacy and safety for bronchial asthma,and the laryngeal discomfort and gastrointestinal reaction should be strengthened.

12.
Article in Chinese | WPRIM | ID: wpr-614337

ABSTRACT

BACKGROUND:Currently, inhaled glucocorticoid is still the classic treatment for asthma. Adult stem cell transplantation has made significant progress in a variety of diseases, and it also provides new insights into the treatment of asthma.OBJECTIVE:To summarize the recent advances in the treatment of asthma with adipose-derived stem cells and related adult stem cells, and to discuss the therapeutic safety of adipose-derived stem cells and possible research directions in asthma therapy.METHODS:Relevant articles published from 2001 to 2016 were searched in PubMed, CBM, CNKI, and Wanfang databases. The keywords were (adipose-derived stem cells[All Fields]) OR (adipose stem cells[All Fields]) OR (adipose-derived mesenchymal stem cells[All Fields]) AND (asthma[All Fields]) in English and Chinese, respectively.RESULTS AND CONCLUSION:A total of 125 literatures were initially searched, and finally 54 representative papers were selected. Adipose-derived stem cells may reduce airway inflammation, airway hyperresponsiveness, ease collagen deposition and scar tissue formation, promote neovascularization, and reconstruct damaged airways in the mouse asthma model through immune regulation. It is necessary to understand its treatment mechanism of action deeply and comprehensively and carry out genomic analysis before introduction of adipose-derived stem cells as a conventional clinical treatment. In summary, adipose-derived stem cells may be a therapeutic potential for the treatment of airway allergic inflammatory diseases such as asthma.

14.
Article in Chinese | WPRIM | ID: wpr-487879

ABSTRACT

Phospholipids and their metabolites play an important role in a variety of cellular processes including cell-cell adhesion, cell growth and differentiation, apoptosis, phagocytosis as well as storage of energy. In this study, the phospholipid composition of cancer tissue and adjacent normal tissue from humans and animals were analyzed by internal extractive electrospray ionization mass spectrometry ( iEESI-MS ) . Extractive solvent at high voltage (+5. 5 kV) was injected into tissue samples using a fused silica capillary at a flow rate of 0. 5-1 μL/min, producing fine charged droplets containing analytes of tissue samples at the tip of the sample. Charged droplets were directly sampled to the atmospheric inlet of a mass spectrometer. Out of 21 different ratios of CH3 OH ∶H2 O solvent mixture, the ratio CH3 OH ∶ H2 O=30∶70 ( V/V ) showed the optimal phospholipids extraction and visibility in MS. A large number of phospholipids from different tissue samples ( such as cancer tissue and adjacent normal tissue of lung cancer, esophageal cancer tissue, pork, beef, porcine heart and porcine lung) were obtained simultaneously by iEESI-MS analysis. The experimental results demonstrated that iEESI-MS was characterized by minimal sample pretreatment, low sample consumption, and rapid analysis ( the analysis time per sample was less than 1 min) , and the selectivity and sensitivity of iEESI-MS could be improved by choosing proper solvent. Importantly, the experimental results provided new information for further studies of phospholipids in biological tissues.

15.
Article in Chinese | WPRIM | ID: wpr-815124

ABSTRACT

Solitary fibrous tumor (SFT) is a derived mesenchymal tumor from spindle cells, mostly occurred in the pleura. To analyze the clinical features of the SFT, data for a patient with SFT that involved in the pleura were retrospectively analyzed by assisted thoracoscope in the Affiliated Hospital of Zunyi Medical College in August 2015. The male patient was 45 years old, who showed the main clinical symptoms of chest pain, cough, sputum, and dyspnea. Large amount of right pleural effusion, chest space-occupying lesions were found by chest CT, suggesting a malignant tumor with metastasis at the 2nd and 3rd right rib. Immunohistochemical results showed: CD34 (+), cytokeratin (-), cytokeratin 5/6 (-), calretinin (-), epithelial membrane antigen(-), mesothelial cell (-), vimentin (++), Wilm's tumor-1 (+), Bcl-2 (+), CD56 (-), CD99 (+), desmin (-), and thyroid transcription factor-1 (-). It was diagnosed as SFT at right side wall layer pleura. SFT is a rare disease and it may occur at any site in the body. It lacks characteristic clinical symptoms and can be asymptomatic, or displays symptoms such as cough, chest pain, dyspnea, and hemoptysis. SFTs can only be conclusively diagnosed based on histopathologic and immunohistochemical characteristics of the tumor, and they are mostly benign. The main treatment for SFTs is the complete surgical resection. The prognosis for this disease is relatively good.


Subject(s)
Calbindin 2 , Humans , Immunohistochemistry , Male , Middle Aged , Prognosis , Solitary Fibrous Tumor, Pleural , Diagnosis , General Surgery , Thyroid Nuclear Factor 1 , Tomography, X-Ray Computed
16.
Chinese Journal of Immunology ; (12): 1019-1023, 2015.
Article in Chinese | WPRIM | ID: wpr-478639

ABSTRACT

Objective:To investigate the effects of CCR3 on Muc5ac in the airway of asthmatic mice.Methods: Fifty clean BALB/c mice were randomly divided into control group,asthmatic group,dexamethasone treatment group,SB328437 treatment group, vehicle-control group.Total cells and differential inflammatory cells were counted in BALF, the levels of IL-4 and TNF-αwere determined by ELISA,lung tissue HE staining the expressions of Mucin5ac(Muc5ac) and CCR3 in lung tissue were detected by immu-neohistochemical staining and RT-PCR.Results:The total cells,eosinophil,monocytes and lymphocyte cells in BALF,the levels of IL-4,TNF-αin BALF ,the goblet cell of airway wall,the expression of Muc5ac and CCR3 positive staining IOD in the airway and the expression of Muc5ac and CCR3 mRNA lung tissue obviously decreased in SB328437 treatment group and DEX treatment group which compared with asthmatic mice model group ( P<0.05 ) .Conclusion: SB328437 can inhibit the expression of CCR3 in pulmonary tissue,furtherly inhibit the expression and secretion of Muc5ac and control the airway inflammation.

17.
Article in Chinese | WPRIM | ID: wpr-452811

ABSTRACT

BACKGROUND:Studies on basic research of magnetic treatment of limb ischemic disease are not much, because poor compliance of animals and the stability of the magnetic field strength are difficult to control, resulting in big experimental error and decreased credibility of the results. For this kind of problem, experimental study on low-frequency electromagnetic magnetic cages for treatment of ischemic limbs was conducted, thus overcoming the two major issues of poor compliance of animals and difficult control of the stability of magnetic field strength. OBJECTIVE:To investigate the effects of self-made low-frequency magnetic fields of rabbit cages on neovascular growth-promoting factor of rabbits with limb ischemia. METHODS:A total of 96 rabbit models of atherosclerosis were constructed, numbered and randomly divided into ischemia group and non-ischemia group (12 treatment combination in each group). Experiments in each group were performed four times according to the requirement of factorial design. Electromagnetic field intensity factor A (0, 3, 6, 12 mT) and the time factor B (3, 5, 7 days) were set. RESULTS AND CONCLUSION:Low-frequency magnetic field could apparently promote hypoxia inducible factor-1α, vascular endothelial growth factor and CD34 expression in ischemic limb of rabbits. Electromagnetic field intensity factor A was a key factor for contributing to the expression of hypoxia inducible factor-1α, vascular endothelial growth factor and CD34, and the time factor B was secondary factor. Low-frequency magnetic field also promoted hypoxia inducible factor-1αexpression in non-ischemia limb, but did not promote vascular endothelial growth factor and CD34 expression. Thus, the expression of vascular endothelial growth factor and CD34 was regulated by hypoxia inducible factor-1α, as wel as other factors, in the ischemic state.

18.
Basic & Clinical Medicine ; (12): 268-271, 2010.
Article in Chinese | WPRIM | ID: wpr-440623

ABSTRACT

Objective Genetic evaluation of relationship between rpoB gene mutation and rifampin-resistance of Mycobacterium tuberculosis (MTB).Methods This study was carded out with 81 clinical isolates and sensitivity test and genotypic analysis by PCR amplification and sequencing of rpoB gene were performed.Results Among these isolates,there were 27 rifampin-resistant and 54 sensitive strains.Of the 27 drug-resistant isolates,20 (74.1%) carried mutations on the amplified fragment of the rpoB gene with 10 mutation types at seven codons including 531 and 526,and two new mutation patterns were recognized.On the uther hand,one mutation (1.9%) appeared in 54 drug-sensitive strains.Conclusion The study shows geographical variation in the mutation types of rpoB gene in M.tuberculosis isolates from Guizhou,China.The result is valuable for the development of fast diagnostic methods.

19.
Article in Chinese | WPRIM | ID: wpr-595805

ABSTRACT

OBJECTIVE To promote the direction,administration and surveillance of nosocomial infection control in general hospital,and to prevent the onset of nosocomial infection and reduce its rate.METHODS Retrospective study was taken based on the data of 95193 cases from Jan 2003 to Jan 2007.RESULTS Nosocomial infection happened in 5024 patients and in 5545 cases-times out of 95193 cases,and the rate was 5.28% and 5.83%,respectively.Infection rate(8.49%)in internal medicine were the highest.The main infection site was lower and upper respiratory tract(the constituent ratio 30.66% and 23.20% respectively).CONCLUSIONS Diminishing or avoiding invasive process,using antibacterial drug rationally,promoting the surveillance of the key department and key patients are very important to control nosocomial infection.

20.
Article in Chinese | WPRIM | ID: wpr-567885

ABSTRACT

Objective:To observe the inhibition of Chuanhong Baliu Paste on S180 tumor and investigate its apoptotic induction. Methods:60 mice were divided into 5 groups randomly after inoculating S180 tumor. S180-bearing mice were smeared with different dosages of Chuanhong Baliu Paste respectively on skin of right armpit. Model group was administrated with normal sodium and positive group was injected intraperitoneally with Cyclophosphamide (25mg/kg) respectively. The growth inhibition was evaluated; apoptotic cells were detected by DNA agarose gelelectrophoresis and flow cytometry. The expression of Caspase-3 was determined by reverse transcription polymerase chain reaction method. Results:Compared with model group,Chuanhong Baliu Paste could signifi cantly inhibit the growth of S180 (P

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