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Angelica dahurica,as a medicinal and edible traditional Chinese medicine,is widely used in clinical practice.It is mainly cultivated in Henan,Hebei,Sichuang and Zhejiang provinces.At present,the study found that the main chemical components of this plant contained coumarins,volatile oil,polysaccharides,alkaloids,and amino acids and other components.It has anti-inflammatory,analgesic,anti-oxidant,anti-tumor,whitening effect and other pharmacological activities.In this paper,the chemical composition,pharmacological action of Angelica dahurica were summarized.Based on the core concepts of quality markers,the quality markers of Angelica dahurica were predicted and analyzed from the aspects of plant relativity and chemical component specificity,traditional medicinal properties,traditional efficacy,measurability of chemical components,Sulfur Fumigated,so as to provide reference for establishment and development of quality evaluation system of Angelica dahurica.
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Objective:To investigate the current situation of health emergency response literacy among emergency nurses, analyze the potential profile of emergency nurses′ health emergency response literacy and explore the influencing factors of different categories, so as to provide reference for formulating targeted and specific training intervention programs.Methods:From September to October 2022, 386 emergency nurses from 5 tertiary A-level hospitals in Shandong Province were selected as the research objects by convenient sampling method. A cross-sectional survey was conducted by using the general information questionnaire, the Literacy Self-assessment Scale of Public Health Emergency Response In Nurses, the Chinese version of Modified Self-leadership Questionnaire, and Nurse Perceived Professional Value Scale. The latent profile analysis was carried out with the results of 3 explicit indexes of the Literacy Self-assessment Scale of Public Health Emergency Response In Nurses. At the same time, the influencing factors of different latent profiles of health emergency literacy were explored by univariate analysis and ordered multi-classification Logistic regression.Results:The health emergency literacy of emergency department nurses was divided into three latent profiles: high health emergency literacy type accounting for 45.4% (175/386), medium health emergency literacy type accounting for 41.5% (160/386), and low health emergency literacy type accounting for 13.1% (51/386). Ordinal Logistic regression showed that the main emergency work post was pre hospital first aid, participated in public health emergency rescue operations, self leadership, and professional value were protective factors of health emergency literacy (all P<0.05). Age≤25 years old and junior college degree were risk factors of health emergency literacy (both P<0.05). Conclusions:Nursing managers can implement personalized training and intervention strategies according to different demographic characteristics of the health emergency literacy of emergency nurses, develop self leadership improvement plans from individual and organizational factors, and actively cultivate their sense of professional value, in order to comprehensively improve the health emergency literacy of emergency nurses.
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Two Diaporthe species isolated from fruit of Citrus sinensis in China were characterized based on morphology and multilocus phylogeny of ITS, tef1, and tub2 gene sequences. The phylogeny indicated that the two species match Diaporthe taoicola and D. siamensis. A critical examination of phenotypic characteristics confirmed the phylogenetic results. Diaporthe taoicola was morphologically characterized by producing Alpha conidia with tapering toward both ends. Meanwhile, D. siamensis produced cylindrical or ellipsoidal Alpha conidia with two oil drops. Pathogenicity tests revealed that both species were pathogenic to fruit of C. sinensis. To our knowledge, the two species were firstly reported on Citrus sinensis in China.
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Two Diaporthe species isolated from fruit of Citrus sinensis in China were characterized based on morphology and multilocus phylogeny of ITS, tef1, and tub2 gene sequences. The phylogeny indicated that the two species match Diaporthe taoicola and D. siamensis. A critical examination of phenotypic characteristics confirmed the phylogenetic results. Diaporthe taoicola was morphologically characterized by producing Alpha conidia with tapering toward both ends. Meanwhile, D. siamensis produced cylindrical or ellipsoidal Alpha conidia with two oil drops. Pathogenicity tests revealed that both species were pathogenic to fruit of C. sinensis. To our knowledge, the two species were firstly reported on Citrus sinensis in China.
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Objective To investigate the role of Yes-associated protein 1 (YAP1)-JUN in promoting the proliferation and epithelial mesenchymal transitions (EMT) transformation of laryngeal squamous cell carcinoma.Methods The expression and subcellular localization of YAP1 were detected by tissue immunofluorescence assay.The effects of YAP1 on the proliferation of laryngeal squamous cell were examined by cell clone formation experiment.Western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were used to detect the effect of YAP1 on the expression and transcriptional level of EMT-related molecular markers.The interaction between YAP1 and JUN was detected by immunocoprecipitation (CoIP) and Western blot assay,which regulated the expression of downstream genes to control the EMT process.Results The expression of YAP1 in laryngeal squamous cell carcinoma tissue increased and transferred from cytoplasm to nucleus.The number of clones increased significantly after YAP1 up regulation (P ＜0.01).The expression level of key gene E-cadherin in epithelial cells was significantly inhibited after YAP1 up (P ＜0.01),while the expression level of the key genes of interstitial cells,β-catenin,vimentin and N-cadherin was significantly up (P ＜ 0.01).YAP1 was interacted with nuclear transcription factor JUN,and the proliferation ability of YAP1 decreased significantly (P ＜0.01) after the inhibition of JUN expression (P ＜ 0.01),and the expression of EMT related molecular markers decreased significantly (P ＜0.01).Conclusions YAP1 combined with JUN gene promotes the proliferation and EMT transformation of human laryngeal squamous cell carcinoma cells.
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Objective@#To investigate the role of Yes-associated protein 1 (YAP1)-JUN in promoting the proliferation and epithelial mesenchymal transitions (EMT) transformation of laryngeal squamous cell carcinoma.@*Methods@#The expression and subcellular localization of YAP1 were detected by tissue immunofluorescence assay. The effects of YAP1 on the proliferation of laryngeal squamous cell were examined by cell clone formation experiment. Western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were used to detect the effect of YAP1 on the expression and transcriptional level of EMT-related molecular markers. The interaction between YAP1 and JUN was detected by immunocoprecipitation (CoIP) and Western blot assay, which regulated the expression of downstream genes to control the EMT process.@*Results@#The expression of YAP1 in laryngeal squamous cell carcinoma tissue increased and transferred from cytoplasm to nucleus. The number of clones increased significantly after YAP1 up regulation (P<0.01). The expression level of key gene E-cadherin in epithelial cells was significantly inhibited after YAP1 up (P<0.01), while the expression level of the key genes of interstitial cells, β-catenin, vimentin and N-cadherin was significantly up (P<0.01). YAP1 was interacted with nuclear transcription factor JUN, and the proliferation ability of YAP1 decreased significantly (P<0.01) after the inhibition of JUN expression (P<0.01), and the expression of EMT related molecular markers decreased significantly (P<0.01).@*Conclusions@#YAP1 combined with JUN gene promotes the proliferation and EMT transformation of human laryngeal squamous cell carcinoma cells.
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<p><b>OBJECTIVE</b>To evaluate the efficacy and safety of the combination therapy of Xipayimaizipizi Capsules and Tamsulo- sin in the treatment of benign prostatic hyperplasia (BPH).</p><p><b>METHODS</b>We randomly assigned 60 BPH patients to a control and a combination group of equal number, the former aged 62.03 ± 10.19 years with a disease course of 3.24 ± 2.18 years and the latter aged 64.77 ± 10.33 years with a disease course of 4.09 ± 2.63 years. We treated the patients in the control group with Tamsulosin at 0.2 mg qd and those in the combination group with Tamsulosin at 0.2 mg qd plus Xipayimaizipizi at 0.5 g tid, respectively, both for 4 weeks. Then, we obtained the mean frequency of nocturnal urination, maximal urinary flow rate (Qmax), residual urine volume, International Prostate Symptom Score (IPSS) , and quality of life scores (QOL) of the patients, and recorded their adverse reactions.</p><p><b>RESULTS</b>Before treatment, the nocturnal urination frequency, Qmax, IPSS, and QOL were 3.60 ± 1.81, (10.40 ± 3.53) ml/min, 22.47 ± 8.58, and 4.43 ± 1.50 in the control group, as compared with 3.43 ± 1.61, (10.14 ± 3.43) ml/min, 21.93 ± 8.79, and 4.73 ± 1.31 in the combination group. After 4 weeks of medication, the combination group showed more significant improvement than the control in the nocturnal urination frequency (1.30 ± 1.18 vs 2.27 ± 1.60), Qmax ([13.85 ± 3.15] vs [14.36 ± 3.03] ml/min), IPSS (13.00 ± 1.53 vs 17.20 ± 8.43), and QOL (2.57 ± 1.61 vs 2.93 ± 1.68), all significantly better than the baseline (P < 0.05). The combination therapy achieved remarkable improvement as compared with the control in the nocturnal urination frequency (- [2.13 ± 1.11] vs -[1.73 ± 1.07]), IPSS (- [8.93 ?6.01] vs -[4.80 ± 3.87]), and QOL (- [2.17 ± 1.12] vs -[1.50 ± 1.01]) (P < 0.05), but exhibited no significant differences from the latter in Qmax ([3.72 ± 2.281 vs [3.95 ± 2.53] ml/min) and residual urine volume (- [34.30 ± 37.43] vs - [26.43 ± 30.49] ml) (P > 0.05). Adverse reactions were found in 5 cases in the combination group (16.67%) and 3 cases in the control (10%) , with no remarkable differences between the two groups (P > 0.05).</p><p><b>CONCLUSION</b>The combination therapy of Xipayimaizipizi Capsules and Tamsulosin can improve the symptoms of BPH and the patients quality of life of.</p>
Subject(s)
Aged , Humans , Male , Middle Aged , Capsules , Drug Therapy, Combination , Drugs, Chinese Herbal , Therapeutic Uses , Prostatic Hyperplasia , Drug Therapy , Quality of Life , Sulfonamides , Therapeutic UsesABSTRACT
Objective To investigate the clinical characteristic of 1 000 Hz probe tone tympanometry obtained from neonates who didn’t pass the TEOAE screening . .Methods TEOAE screening were performed to screen the hearing with GSI -70 Automated OAE(Grason-Stadler ,USA) .Then neonates were detected the 1 000 Hz probe tone tympanograms by GSI Tympstar Version Ⅱ Middle Ear Analyzer(Grason-Stadler ,USA) .The relative infor-mation ,including tympanometric peak pressure (Tpp) ,peak admittance(Yp) ,peak compensated static acoustic ad-mittance (Ypc)、tympanometric width(TW) were collatea .The of 1 000 Hz probe tone tympanometry of 47 ears that didn’t pass the first TEOAE and 1 300 ears that passed the first TEOAE were asnpared .Results We found in the single-peak type ,the ratio of the experimental group(59 .57% ) was less than that of the control group(74 .00% ) , and in the flat type the ratio of the the experimental group (23 .40% ) was more than that of the control group (8 . 54% ) ,there was a statistical significance between the two groups in each different type proportion (P<0 .05) .But there is not a statistical significance in the relative values of single -peak type admittance between the two groups , such as tympanometric peak pressure (Tpp)、peak admittance(Yp) etc .Conclusion The majority of 1000Hz tympa-nograms of the neonates who passed TEOAE is the single -peak type ,but the neonates who didn’t pass the first TEOAE mostly shored the flat type The .
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OBJECTIVE: To establish the UPLC fingerprint of Ziziphi Spinosae Semen. METHODS: The UPLC fingerprint of 14batches of Ziziphi Spinosae Semen were determined on an HSS T3 column (2.1 mm × 100 mm, 1.8 μm) eluted with the mobile phase consisting of 0.05% phosphoric acid solution and acetonitrile in gradient mode: the flow rate was 0.5 mL · min-1; the column temperature was 30°C and the detection wavelength was set at 204 nm. RESULTS: The common mode of the UPLC fingerprint of Ziziphi Spinosae Semen was set up under the established condition. There were 17 common peaks in the fingerprints of 14 samples, five of which were identified. CONCLUSION: The method is rapid and accurate. The chromatographic profile of Ziziphi Spinosae Semen with high specificity can be used to control the quality of Ziziphi Spinosae Semen.
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<p><b>OBJECTIVE</b>To isolate, culture and identify odontoclasts in vitro and to establish a method of culturing human odontoclasts.</p><p><b>METHODS</b>Healthy and retentive deciduous teeth were extracted, and then placed in α-minimum essential medium containing 0.1% collagenase and 0.2% dispase for 1 h.Odontoclasts were obtained and incubated from the absorbing root surfaces of deciduous teeth.Isolated cells were viewed by inverted phase contrast microscope firstly. Then, the isolated odontoclasts were morphologically observed by hematoxylin and eosin staining (HE) and tartrate-resistant acid phosphatase (TRAP) staining. The prepared teeth slices were cocultured with the isolated odontoclasts and scanning electronic microscope(SEM) was used to demonstrate the presence of resorption lacunae.</p><p><b>RESULTS</b>The isolated odontoclasts appeared as multinucleated giant cell with many vacuolus in cytoplasm. TRAP staining demonstrated that the cytoplasm of the odontoclasts was full of claret-red positive particles.Resorption lacunae on teeth slices which cocultured with odontoclasts were seen under SEM.</p><p><b>CONCLUSIONS</b>Enzyme digestion is an effective method to isolate odontoclasts from absorbing root surface of deciduous teeth.</p>
Subject(s)
Child , Child, Preschool , Humans , Acid Phosphatase , Metabolism , Cells, Cultured , Giant Cells , Cell Biology , Metabolism , Isoenzymes , Metabolism , Microscopy, Phase-Contrast , Osteoclasts , Cell Biology , Metabolism , Root Resorption , Staining and Labeling , Methods , Tartrate-Resistant Acid Phosphatase , Tooth Root , Cell Biology , Tooth, Deciduous , Cell BiologyABSTRACT
A laccase gene (lacD) from the basidiomycete Trametes sp. 420 was heterologously expressed in Pichia pastoris in two ways, resulting in two recombinant enzymes of rLacDx with native N-terminus and rLacDe with eight additional amino acid residues at N-terminus. The yields of rLacDx and rLacDe in shaken-flask cultures after an 18-day growth were 1.21 x 10(5) u/L and 7.38 x 10(4) u/L, respectively, as determined with 2,2'-azinobis(3-ethylbenzothia-zoline- 6-sulfonic acid) (ABTS) as substrate. The yield of rLacDx was further increased to 2.39 x 10(5) u/L under high-density fermentation while the production process was decreased to 7.5 days. In addition, rLacDx and rLacDe exhibited similar enzymatic characters in oxidizing substrate guaiacol, and were stable at 50 degrees C and at a pH range from 3 to 10. However, the specific activity of rLacDx (1761 u/mg) for ABTS was higher than that of rLacDe (1122 u/mg), and the apparent Km value of rLacDx (427 microM) was less than that of rLacDe (604 microM).
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Cloning, Molecular , Fermentation , Isoenzymes , Genetics , Laccase , Genetics , Pichia , Genetics , Metabolism , Recombinant Proteins , Genetics , Trametes , GeneticsABSTRACT
Objective To observe the effect of ginkolide B derivative(XQ) on animal thrombosis.Methods SD male rats were randomized into the model group,troxerutin(48 mg?kg-1?d-1) group,ginkolide B(7.8 mg?kg-1?d-1) group,and low-,middle-and high-dose XQ(in the dose of 3.9,7.8 and 15.6 mg?kg-1?d-1 respectively) groups.The anti-thrombotic effect of XQ was observed on rats mixed thrombosis model induced by arterio-venous shunt method and electrical stimulation.Meanwhile,ICR male mice were randomized into the model group,troxerutin(96 mg?kg-1?d-1) group,ginkolide B(15.6 mg?kg-1?d-1) group,and low-,middle-and high-dose XQ(in the dose of 7.8,15.6 and 31.2 mg?kg-1?d-1 respectively) groups.The anti-thrombotic effect of XQ was also observed on mice acute pulmonary embolism model induced by adenosine diphosphate(ADP).Results XQ at the dose of 7.8 and 15.6 mg?kg-1?d-1 decreased the dry and wet weight of the thrombosis,and obviously prolonged the formation time of rats carotid artery thrombosis as compared to the model group(P