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1.
Article in Chinese | WPRIM | ID: wpr-828937

ABSTRACT

OBJECTIVE@#To explore the effects of taurolithocholic acid (tLCA) and chenodeoxycholic acid (CDCA) on the expression of aorexigenic neuropeptide in mouse hypothalamus GT1-7 cells.@*METHODS@#Mouse hypothalamic GT1-7 cells were treated with culture medium containing 10% FBS (control group, =3) or with 10 nmol/L, 100 nmol/L, 1 μmol/L and 10 μmol/L tLCA (tLCA group, =3) or CDCA (CDCA group, =3) for 12, 24 or 48 h. Real-time PCR was performed to determine the expression levels of proopiomelanocortin (POMC) mRNA in the cells, and the production levels of α-melanocyte-stimulating hormone (α-MSH) were assessed using an ELISA kit. Signal transduction and activator of transcription 3 phosphorylation (p-STAT3), threonine kinase phosphorylation (p-AKT), suppressor of cytokine signaling 3 (SOCS3), G protein-coupled bile acid receptor-1 (TGR5) and farnesoid X receptor (FXR) protein were detected by Western blotting.@*RESULTS@#Western blotting results showed that mouse hypothalamic GT1-7 cells expressed two bile acid receptors, TGR5 and FXR, whose expressions were regulated by bile acids. Real-time PCR showed that the expression of POMC mRNA was significantly increased in the cells after treatment with 10 μmol/L tLCA or CDCA for 24 h. POMC-derived anorexigenic peptide α-MSH increased significantly in GT1-7 cells after treatment with 10 μmol/L tLCA or CDCA for 24 h. Treatment of the cells with tLCA or CDCA significantly increased the expressions of intracellular signaling proteins including p-STAT3, p-AKT and SOCS3.@*CONCLUSIONS@#Mouse hypothalamic GT1-7 cells express bile acid receptors TGR5 and FXR. Bile acids tLCA or CDCA can promote the expression of POMC mRNA and increase the production of the anorexigenic peptide α-MSH. The intracellular signaling proteins p-AKT, p-STAT3 and SOCS3 are likely involved in bile acid-induced anorexigenic peptide production.


Subject(s)
Animals , Bile Acids and Salts , Chenodeoxycholic Acid , Hypothalamus , Mice , Neuropeptides , Phosphorylation , STAT3 Transcription Factor , Signal Transduction , Suppressor of Cytokine Signaling 3 Protein
2.
Article in Chinese | WPRIM | ID: wpr-828518

ABSTRACT

OBJECTIVE@#To explore the effects of taurolithocholic acid (tLCA) and chenodeoxycholic acid (CDCA) on the expression of aorexigenic neuropeptide in mouse hypothalamus GT1-7 cells.@*METHODS@#Mouse hypothalamic GT1-7 cells were treated with culture medium containing 10% FBS (control group, =3) or with 10 nmol/L, 100 nmol/L, 1 μmol/L and 10 μmol/L tLCA (tLCA group, =3) or CDCA (CDCA group, =3) for 12, 24 or 48 h. Real-time PCR was performed to determine the expression levels of proopiomelanocortin (POMC) mRNA in the cells, and the production levels of α-melanocyte-stimulating hormone (α-MSH) were assessed using an ELISA kit. Signal transduction and activator of transcription 3 phosphorylation (p-STAT3), threonine kinase phosphorylation (p-AKT), suppressor of cytokine signaling 3 (SOCS3), G protein-coupled bile acid receptor-1 (TGR5) and farnesoid X receptor (FXR) protein were detected by Western blotting.@*RESULTS@#Western blotting results showed that mouse hypothalamic GT1-7 cells expressed two bile acid receptors, TGR5 and FXR, whose expressions were regulated by bile acids. Real-time PCR showed that the expression of POMC mRNA was significantly increased in the cells after treatment with 10 μmol/L tLCA or CDCA for 24 h. POMC-derived anorexigenic peptide α-MSH increased significantly in GT1-7 cells after treatment with 10 μmol/L tLCA or CDCA for 24 h. Treatment of the cells with tLCA or CDCA significantly increased the expressions of intracellular signaling proteins including p-STAT3, p-AKT and SOCS3.@*CONCLUSIONS@#Mouse hypothalamic GT1-7 cells express bile acid receptors TGR5 and FXR. Bile acids tLCA or CDCA can promote the expression of POMC mRNA and increase the production of the anorexigenic peptide α-MSH. The intracellular signaling proteins p-AKT, p-STAT3 and SOCS3 are likely involved in bile acid-induced anorexigenic peptide production.


Subject(s)
Animals , Cell Line , Chenodeoxycholic Acid , Pharmacology , Gene Expression Regulation , Hypothalamus , Cell Biology , Mice , Neuropeptides , Genetics , Metabolism , Pro-Opiomelanocortin , Genetics , RNA, Messenger , Genetics , STAT3 Transcription Factor , Metabolism , Signal Transduction , Suppressor of Cytokine Signaling 3 Protein , Metabolism , Taurolithocholic Acid , Pharmacology , alpha-MSH , Genetics
3.
Article in Chinese | WPRIM | ID: wpr-616415

ABSTRACT

Objective To explore the application effect of debate teaching in food toxicology teaching.Methods Taking the chapter of mutagenic effect of exogenous chemicals in the course of food toxicology as an example,totally hygiene quarantine specialty undergraduate students from March 2015 to July 2016 in our school for the course of Grade 2014 (n=37),Grade 2013 (n=49) were selected as subjects and conducted debate teaching and traditional teaching comparison.The students of Grade 2014 took the traditional teaching method as the control group,while the students of Grade 2013 took the debate teaching method as the experimental group.After the teaching of two groups,quizzes were used.to evaluate the students' learning of this course.In addition,the students in the debate teaching group were investigated by the questionnaire of the students' satisfaction with the teaching method and the learning performance of the students in the teaching practice at the end of the quiz.T test was conducted on the test scores of two groups of students by SPSS 16.0.Results The average test score of the students in the experimental group was higher than that of the control group [(7.24 ± 0.66) vs.(6.35 ± 0.82),t=5.575,P=0.000].49 questionnaires were issued to the students in the experimental group,and 49 valid questionnaires were recovered.The majority of students believed that the debate teaching method aroused their interest in the course of learning and enhanced their abilities and qualities.Conclusions The application of debate teaching in food toxicology teaching has good effect,and it can train students' comprehensive ability.

4.
Chinese Journal of Epidemiology ; (12): 418-424, 2016.
Article in Chinese | WPRIM | ID: wpr-237529

ABSTRACT

Objective To systematically evaluate the etiological association between esophagogastric junction adenocarcinoma and Helicobacter pylori (Hp) infection and the pathogenicity of Cag pathogenic island in Hp infection cases.Methods Literatures about Hp infection and esophagogastric junction adenocarcinoma published from January 1980 to April 2015 were retrieved from CNKI,Wanfang data,VIP,the Cochrane Library,PubMed,EMBase databases.The literatures which met the inclusion criteria were collected and evaluated by using Newcastle-Ottawa Scale,then the heterogeneity was analyzed with Review Manager 5.3,and the pooled OR value and 95% confidence interval were calculated.Results A total of 5 547 study subjects were recruited in 13 studies,including 1 446 esophagogastric junction adenocarcinoma cases and 4 101 controls.The combined OR for Hp infection was 0.95 (95%CI:0.66-1.36),P=0.71;The OR in high risk areas was 1.66 (95%CI:1.33-2.08,P<0.001),higher than that in low-risk areas (0.68,95%CI:0.49-0.94,P=0.020).In addition,six studies found that the combined detection rates of Cag pathogenic island in esophagogastric junction adenocarcinoma cases and controls were 80.50% and 79.80%,respectively.There was no significant difference between two group,the combined OR was 1.24 (95%CI:0.96-1.60).Conclusion The association between Hp infection and esophagogastric junction adenocarcinoma was not significant,however,the significant difference was observed between high risk area and low risk area;the detection rate of Gag pathogenic island in Hp infection cases was high,but the pathogenicity for esophagogastric junction adenocarcinoma needs further study.

5.
Chinese Pharmacological Bulletin ; (12): 785-789, 2015.
Article in Chinese | WPRIM | ID: wpr-463248

ABSTRACT

Aim To identify alteration in key molecular components related to memory formation and insulin signaling in the hippocampus after rosiglitazone was in-jected into the ob/ob mice to test whether cognitive dysfunction was pharmacologically reversed by regula-tion of rosiglitazone. Methods The age-matched mice were divided into three groups ( n=18 ): Saline-trea-ted WT mice ( WT-Saline);Saline-treated ob/ob mice ( ob/ob-Saline) and RSG-treated ob/ob mice ( ob/ob-RSG) through intraperitoneal injection of rosiglitazone ( RSG) . The random glucose levels were measured for 10 days during the intraperitoneal injection period. No-vel object recognition was performed before mice were sacrificed. Western blot was implemented to evaluate the following proteins: BACE1, p-Tau, p-IRS1,IRS1, p-Akt and Akt in hippocampal tissues. The Aβ1-40 levels were detected by ELISA Kit. Results The random blood glucose levels were significantly re-duced in ob/ob-RSG compared with ob/ob-saline. RSG treatment led to an increase in hippocampus-de-pendent cognition of ob/ob mice according to the novel object recognition. The proteins levels of BACE1, p-Tau and Aβ were lowered in RSG-treated ob/ob mice. Furthermore, RSG treatment up-regulated hippocampal p-IRS1/IRS1 and p-Akt/Akt ratio. Conclusion Ros-iglitazone ameliorates cognitive deficits in ob/ob mice through up-regulating insulin signaling pathways in the hippocampus.

6.
Article in Chinese | WPRIM | ID: wpr-408476

ABSTRACT

BACKGROUND: At present, there are fewer studies on the monitoring of nutritional status of the residents living in the draw-down area of ThreeGorge reservoir.OBJECTIVE: To investigate the nutritional status and dietary pattern of the residents before the formation of the draw-down area of Three-Gorge reservoir, so as to provide basic data for the continuous dynamic surveillance of food and nutrition.DESIGN: A cross-sectional study.SETTING: Department of Nutrition and Food Hygiene, School of Public Health, Chongqing University of Medical Sciences.PARTICIPANTS: The investigation was carried out between February and March 2004 in the draw-down area of Three-Gorge reservoir with denser population, larger area and greater influence, such as Kaixian and Yunyang counties, etc. The first surveillance of food nutritional status of the residents in the draw-down area was performed in 52 urban families and 50 rural families of Kaixian county, one family member was voluntarily selected from each family, whose main duty was to buy vegetables and food. Totally 102 subjects (42 males and 60 females) were investigated in this study. At the same time, the data of physical examination and detection of blood Hb in children from Kaixian county between May 1999 and November 2003 were analyzed.METHODS: The subjects were investigated in their house with questionnaires. Their general condition of family, food consumption and dietary nutrition were surveyed and analyzed. Nutrients intake was assessed by 24-hour recall, combined with food consuming frequency questionnaire, the Engel index, which was an important index for the level of family consumption, was detected in both rural and urban residents. Engel index=cost in food/total family income×100%.MAIN OUTCOME MEASURES: ① Amount of food consumption, intakes of energy and nutrients in residents of the draw-down area; ② Prevalence rate of low body mass and rate of growth retardation in children of the draw-down area.RESULTS: All the 102 residents of the draw-down area entered the analysis of results without deletion. ① Amount of food consumption in residents of the draw-down area: Among the urban residents of the draw-down area, the per capita food consumption was 243 g per day, and the consumptions of vegetable, fruit and fish were lower than the average national level, but that of grease food showed a higher level. Among rural residents of Kaixian county, the per capita food consumption was 268 g per day, and the consumption of foodstuff was obviously decreased, but those of meet and grease food showed an ascending trend. ② Energy intake in residents of the draw-down area: Among urban residents of Kaixian county, the per capita energy intake was 6 909 kJ per day, which was obviously lower than that in the urban residents of Chongqing city in 1998 and the average national urban level. The per capita energy intake in rural residents of Kaixian county was 7 577 kJ per day, which was 48.4% lower than that in the urban residents of Chongqing city in 1998 and 37% lower than the average national rural level. ③ Nutritional status in children of the draw-down area: The incidence rate of low body mass in children younger than 5 years in 1999 was close to the average national level (9.6%), it was obviously decreased from 2000, and then fluctuated between 1.0% and 4.8%. Since 1999, the incidence rate of growth retardation in children younger than 5 years of Kaixian county showed a gradually descending trend, but anemia and other common diseases still kept a higher incidence rate, the detection rate of anemia was 33.5% in 1999, and decreased to 20.8% in 2003.CONCLUSION: The current nutritional status of the Kaixian people living in draw-down area of Three-Gorge reservoir still needs further improvement, and dynamic food and nutrition surveillance in succession is necessary.

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