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1.
Article in Chinese | WPRIM | ID: wpr-885343

ABSTRACT

Rehabilitation medicine is one of the most important specialties in community health institutions. This article introduces the 12 year′s development of rehabilitation medicine in Fenglin Community Health Service Center, focusing on the talent allocation, service capabilities, resource expansion, basic facilities, personnel recruiting, department operating, service scope, and its achievements and influence, to provide reference for planning and construction of featured specialty in community health service centers.

2.
China Pharmacy ; (12): 62-66, 2020.
Article in Chinese | WPRIM | ID: wpr-817379

ABSTRACT

ABSTRACT OBJECTIVE:To determine the suitable storage conditions,packaging materials and packaging methods of Ziziphi Spinosae Semen,and to provide reference for guaranteeing the quality. METHODS:The same batch of Ziziphi Spinosae Semen samples were packed in plastic woven bag,plastic bag,plastic bag in vacuum,aluminum-plastic composite bag,aluminum-plastic composite bag in vacuum and kraft coated paper bag. They were stored in a cool warehouse(temperature ≤20 ℃,relative humidity was 45%-75%) and drug stability test box [temperature was(40±2)℃,relative humidity was(75±5)%]. Five evaluation indexes were detected during 6-month of long-term cool stability test and accelerated stability test,including characters and the contents of moisture,aflatoxins,jujubosides A and spinosin. RESULTS:The results of 6-month long-term cool stability test showed that the character and the content of moisture,aflatoxins and jujubosides A of Ziziphi Spinosae Semen under different packaging conditions were in line with the requirements of 2015 edition of Chinese Pharmacopeia (part Ⅰ)(shorted for “pharmacopeia”). The content of spinosin didn’t meet the requirement of pharmacopeia(no less than 0.080%),but the content of spinosin(0.079%)of aluminum-plastic composite bag in vacuum was close to the requirements of pharmacopeia. The results of 6-month accelerated stability test showed that the sample packed in kraft coated paper bag and plastic woven bag were seriously moldy;the sample packed in plastic bag and plastic bag in vacuum were blackened;the color of the sample packed in aluminum-plastic composite bag was slightly darker;but the appearance of the sample packed in aluminum-plastic composite bag in vacuum was basically unchanged. Only when stored for 2-month,the content of aflatoxin B1 in the sample packed in plastic woven bag was 8.64 μg/kg,which exceeded the pharmacopoeia regulation. The moisture content of the sample packed in plastic woven bag and kraft coated paper bag exceeded the highest value (9.0%) required by the pharmacopoeia. Although the content of jujuboside A of samples in each package decreased,it meet the requirements of the pharmacopoeia. Only the content of spinosin (0.084%)of sample packed in plastic bag in vacuum meet the requirements of the pharmacopoeia,and the content of spinosin (0.071%)of sample packed in aluminum-plastic composite bag in vacuum was relatively high. CONCLUSIONS:Ziziphi Spinosae Semen should be stored in a cool and dry place after packed in aluminum-plastic composite bag in vacuum.

3.
Protein & Cell ; (12): 104-119, 2019.
Article in English | WPRIM | ID: wpr-757937

ABSTRACT

Arrhythmogenic right ventricular dysplasia/cardiomyopathy (ARVD/C) is a genetic cardiac muscle disease that accounts for approximately 30% sudden cardiac death in young adults. The Ser358Leu mutation of transmembrane protein 43 (TMEM43) was commonly identified in the patients of highly lethal and fully penetrant ARVD subtype, ARVD5. Here, we generated TMEM43 S358L mouse to explore the underlying mechanism. This mouse strain showed the classic pathologies of ARVD patients, including structural abnormalities and cardiac fibrofatty. TMEM43 S358L mutation led to hyper-activated nuclear factor κB (NF-κB) activation in heart tissues and primary cardiomyocyte cells. Importantly, this hyper activation of NF-κB directly drove the expression of pro-fibrotic gene, transforming growth factor beta (TGFβ1), and enhanced downstream signal, indicating that TMEM43 S358L mutation up-regulates NF-κB-TGFβ signal cascade during ARVD cardiac fibrosis. Our study partially reveals the regulatory mechanism of ARVD development.

4.
Neuroscience Bulletin ; (6): 4-14, 2019.
Article in English | WPRIM | ID: wpr-775443

ABSTRACT

The pathophysiology of visceral pain in patients with irritable bowel syndrome remains largely unknown. Our previous study showed that neonatal maternal deprivation (NMD) does not induce visceral hypersensitivity at the age of 6 weeks in rats. The aim of this study was to determine whether NMD followed by adult stress at the age of 6 weeks induces visceral pain in rats and to investigate the roles of adrenergic signaling in visceral pain. Here we showed that NMD rats exhibited visceral hypersensitivity 6 h and 24 h after the termination of adult multiple stressors (AMSs). The plasma level of norepinephrine was significantly increased in NMD rats after AMSs. Whole-cell patch-clamp recording showed that the excitability of dorsal root ganglion (DRG) neurons from NMD rats with AMSs was remarkably increased. The expression of β adrenergic receptors at the protein and mRNA levels was markedly higher in NMD rats with AMSs than in rats with NMD alone. Inhibition of β adrenergic receptors with propranolol or butoxamine enhanced the colorectal distention threshold and application of butoxamine also reversed the enhanced hypersensitivity of DRG neurons. Overall, our data demonstrate that AMS induces visceral hypersensitivity in NMD rats, in part due to enhanced NE-β adrenergic signaling in DRGs.


Subject(s)
Adrenergic Agents , Pharmacology , Animals , Ganglia, Spinal , Hyperalgesia , Drug Therapy , Hypersensitivity , Drug Therapy , Male , Maternal Deprivation , Neurons , Patch-Clamp Techniques , Methods , Rats, Sprague-Dawley , Signal Transduction , Stress, Physiological , Physiology , Visceral Pain , Metabolism
5.
Article in Chinese | WPRIM | ID: wpr-802016

ABSTRACT

The commodity specification and grade is a symbol of quality of Chinese medicinal materials,a standard to measure and evaluate the quality of Chinese medicinal materials,and an important part of the quality control system of Chinese medicinal materials.This article reviewed relevant research literature of commodity specification and grade of Chinese medicinal materials,including the formation and policy support of specification and grade.It analyzed the specific research examples of the commodity specification and grade of Chinese medicinal materials according to the classification of medicinal parts,and summarized the research conditions of the commodity classification at present stage.Aiming at the current research difficulties and the lack of unified standards in specification and grade of Chinese medicinal materials,some advice including research objects,methods,management,publicity,promotion and application are put forward,so as to provide references for the research on the commodity specification and grade of Chinese medicinal materials.

6.
Article in Chinese | WPRIM | ID: wpr-801984

ABSTRACT

Classical traditional Chinese medicine(TCM) excellent prescriptions is an outstanding representative in prescriptions of traditional Chinese medicine,clinical medicine effective tool,and the essence of traditional culture of Chinese medicine.The research and development of classical TCM excellent prescriptions has attracted great attention from group in the whole industry chain of TCM,a series of guiding suggestions and targeted literature laid a good foundation for research and development of classical TCM excellent prescriptions.In this paper,based on the CNKI database,literature about suggestions or enlightenments of classical TCM excellent prescriptions was sorted and refined in order to build the research outline of classical TCM excellent prescriptions,and it was concluded that literature research was the premise,medicine/decoction pieces with good quality was the foundation,standard substance was the core,high quality was the guarantee and preferential policies were the impetus,and related discussions were carried out.All these were expected to promote the research of classical TCM excellent prescriptions and development of related industries.

7.
Article in Chinese | WPRIM | ID: wpr-801807

ABSTRACT

Objective: To establish the grade evaluation standard for Magnoliae Officinalis Cortex processed with ginger juice by combining traditional morphology evaluation with modern intrinsic quality evaluation. Method: The morphological parameters and contents of intrinsic pharmacodynamic index components of 28 batches of Magnoliae Officinalis Cortex processed with ginger juice were determined, and the relative quality constants were calculated. Assuming that the average relative quality constant was 100%, more than 120%of the samples were classified as the first grade, 60%to 120%as the second grade, the remaining as the third grade. Result: The relative quality constant of Magnoliae Officinalis Cortex processed with ginger juice ranged from 0.09 to 1.78. The relative quality constant of the first grade was ≥ 0.64, the second grade was 0.32-0.64, while the third grade was ≤ 0.32. Conclusion: Relative quality constant combines external indexes of traditional morphology and internal indexes of pharmacodynamic components, which can objectively, reasonably and scientifically classify the grade of Magnoliae Officinalis Cortex processed with ginger juice, and provide reference for establishing and improving the grade evaluation standard of this decoction pieces.

8.
Article in Chinese | WPRIM | ID: wpr-701550

ABSTRACT

Objective To understand biofilm-forming ability of Acinetobacter baumannii(A.baumannii) in clinical isolates,and evaluate the effect of biofilm formation on antimicrobial resistance.Methods A total of 47 A.baumannii clinical isolates were collected,biofilm-forming ability was detected by crystal violet staining assay,the minimal inhibitory concentration(MIC) of 12 kinds of antimicrobial agents to biofilrn forming isolates were detected when the isolates were in planktonic or biofilm formatted condition;biofilm-forming ability of positive isolates at different culture condition (shaking or stable) and on different materials(glass tube or polypropylene plastic centrifugal tube) were detected.Results Thirteen isolates (27.66%) exhibited biofilm formation.In planktonic condition,antimicrobial resistance rates of these isolates were all <35%,no isolates shown resistance to polymyxin and tigecycline,in biofilm formatted condition,resistance rates to tigecycline was 15.38%,resistance rates to the other antimicrobial agents were all 100%.Of 13 tested isolates,≥10 isolates shown higher absorbance in the shaking condition than in the stable condition,≥10 tested isolates shown higher absorbance in polypropylene plastic centrifugal tube than in the glass tube.Conclusion The resistance of A.baumannii to antimicrobial agents is greatly changed after the formation of biofilm,which may results in the failure of antimicrobial therapy for infection.

9.
Article in Chinese | WPRIM | ID: wpr-708137

ABSTRACT

Objective To investigate the effects of titanium dioxide ( TiO2 ) nanoparticles coupled with nuclear localization sequence ( NLS ) on the radiosensitivity of U251 glioma cells. Methods Synthesis and characterization of the TiO2-NLS nanoparticles with nuclear targeting property. U251 cells were treated with nanoparticles and/or ionizing radiation. Flow cytometry analysis was performed to measure the ROS content and the cell apoptotic percentage. The DNA damage was detected by using γ-H2AX foci staining. Clonogenic survival assay was used to evaluate the radiosensitivity of U251 cells. Results NLS modification promoted nuclear translocation of TiO2 nanoparticles. Compared with the control nanoparticles, TiO2-NLS treatment increased radiation-induced cell apoptosis (t=8. 96, P<0. 05). Clonogenic survival assay showed the radiosensitization ratios of TiO2 nanoparticle-treated group and TiO2-NLSnanoparticle-treated group were 1. 18 and was 1. 29, respectively. These two ratios had statistically difference ( t =14. 72, P< 0. 05). Conclusions Nuclear targeting TiO2 nanoparticles enhances the radiosensitivity of U251 glioma cells.

10.
Chinese Journal of Anesthesiology ; (12): 1369-1371, 2018.
Article in Chinese | WPRIM | ID: wpr-745612

ABSTRACT

Objective To evaluate the value of the ratio of optic nerve sheath diameter to eyeball transverse diameter (ONSD/ETD) in assessing intracranial pressure using ultrasound in the patients with brain injury.Methods Forty-six patients of both sexes with brain injury in the surgical intensive care unit,were selected.Craniotomy was performed within 24 h after admission to hospital,and the intracranial pressure probe was placed in the lateral cerebral ventricle of the enrolled patients who were aged 18-80 yr,with Glasgow Coma Scale score 3-15.ONSD and ETD were measured within 3 days after operation,three times a day,and the intracranial pressure was simultaneously recorded.The correlation between ONSD/ETD ratio and intracranial pressure were analyzed using the Pearson correlation analysis.The receiver operating characteristic curve was used to evaluate the accuracy of ONSD/ETD ratio in assessing the intracranial hypertension (intracranial pressure>20 mmHg).Results The ONSD/ETD ratio was positively correlated with intracranial pressure (r =0.720,P<0.01).The area under the receiver operating characteristic curve was 0.900 (95% confidence interval 0.854-0.946),and the threshold was 0.248 (sensitivity 89.4%,specificity 77.5%).Conclusion The ONSD/ETD ratio produces higher accuracy in assessing intracranial pressure in the patients with brain injury.

11.
Article in Chinese | WPRIM | ID: wpr-702528

ABSTRACT

Objective To study the dynamic changes of the intestinal function of neurogenic bowel dysfunction rats caused by spi-nal cord injury using X-ray radiography. Methods Twenty-four female Sprague-Dawley rats were divided into control group (n=12) and spinal cord injury group (n=12). The T10spinal cord injury model was established using aneurysm clip (70 grams calibration force) for 60 seconds. The control group exposed the dura only. X-ray Barium was used to observe the dynamic changes of in-testinal function, and HE staining was used to observe the pathology of the colon before and four weeks after modeling. Results Compared with the control group, gastric emptying and intestinal transit function significantly reduced in the spinal cord injury group (P<0.05). Conclusion The spinal cord injury model can be successfully duplicated with aneurysm clip in rats; neurogenic bowel dysfunction occurs after spinal cord injury, gastric emptying and intestinal transit function are weakened.

12.
Article in Chinese | WPRIM | ID: wpr-702519

ABSTRACT

Objective To further explore the complication of spinal cord injury occured after transcatheter arterial chemoemboliza-tion(TACE)for hepatocellular carcinoma via inferior phrenic artery. Methods The clinical and imaging data of one patient with spinal cord injury caused by TACE for hepatocellular carci-noma via inferior phrenic artery was retrospectively analyzed.The pathologic mechanism,risk factors and out-come of rehabilitative treatment were discussed after literature review. Results The abnormal opening of communicating branches between inferior phrenic artery and spinal cord blood supply-ing was the underlying mechanism inducing spinal cord injury after TACE. The risk factors included contrast agent,chemotherapy drug,embolic agent,and multiple interventional therapy,etc.Most patients returned almost to normal state within several months after early rehabilitation. Conclusion Spinal cord injury occurred after TACE for hepatocellular carcinoma via inferior phrenic artery is an ex-tremely rare complication.Most patients have a favorable outcome with early detection and timely rehabilitation.

13.
Article in Chinese | WPRIM | ID: wpr-702505

ABSTRACT

Neurogenic bowel dysfunction is one of the most common complications of spinal cord injury.It can cause abdominal distension,constipation,fecal incontinence,difficult defecation,prolonged defecation and other symptoms.At present, neurogenic bowel dysfunction is mainly evaluated through general condition,physical examination,experimental exami-nation,professional scales and intestinal microbiota,etc.

14.
Article in Chinese | WPRIM | ID: wpr-702466

ABSTRACT

Objective To retrospectively analyze the features and related factors of deep venous thrombosis(DVT)in patients with traumatic paraplegia during early rehabilitation. Methods Inpatients of our hospital from June,2014 to June,2017 were included and patients with traumatic paraplegia during early rehabilitation were analyzed.The clinical information,lower limbs deep vein ultrasonic testing and laboratory examination were collected to analyze features and related factors of DVT. Results A total of 269 patients with traumatic paraplegia during early rehabilitation were analyzed and 62 patients had DVT in lower limbs(23.0%),in which 50 patients had an isolated distal DVT and 28 cases occurred in bilateral lower limbs(45.2%).No patients younger than 14 had DVT in lower limbs(n=31).Logistic regression analysis showed that increased D-dimer (OR=1.348, 95% CI 1.193~1.525), advanced age (OR=3.450, 95% CI 1.372~8.674),male(OR=2.872,95% CI 1.095~7.533)and diabetes(OR=5.319,95% CI 1.094~25.872)were indepen-dent related factors for DVT. Conclusion The incidence of DVT in lower limbs of traumatic paraplegia patients during early rehabilitation is high. DVT develops mainly distally to the popliteal vein and bilaterally of lower limbs. Prepubertal children have a low incidence of DVT.Increased D-dimer,advanced age,male and diabetes are independent related factors for DVT.

15.
Article in Chinese | WPRIM | ID: wpr-752126

ABSTRACT

Objective: To establish the method of simultaneous determination of contents of honokiol, magnolol and piperitylmagnolol in Cortex Magnoliae Officinalis from different regions, which could provide evidence for the quality control evaluation of Cortex Magnoliae Officinalis. Methods: The quantitative analysis was performed with a column of Waters Acquity UPLC BEH-Cl8 (2.1 mm×50 mm, 1.7 μm) by UPLC-PDA, and eluted with a mobile phase of 0.2%formic acid solution (A)-methanol (B) in a gradient mode (0-2 min, 65%-65% B; 2-3 min, 65%-75% B; 3-5 min, 75%-84% B; 5-9 min, 84%-90% B) under a flow rate of 0.5 mL·min-1. The column temperature was set at 35℃, and the detection wavelength was 294 nm and the injection volume was 1 μL. Results: The 3 lignan components were completely separated and could be separated well from other components. The honokiol, magnolol and piperitylmagnolol showed a good linear relationship with chromatographic peak area in range of 20.3-406.0, 15.2-304.0, 5.6-112.0 ng, respectively.The average recoveries were 91.75%, 93.86%, 95.15% with the RSDs were 1.75%, 1.88%, 1.91% (n = 9), respectively.Conclusion: Contents of the 3 constituents from different place were significantly different and this method is simple and effective, which can be used to quality control of Cortex Magnoliae Officinalis.

16.
Article in Chinese | WPRIM | ID: wpr-611952

ABSTRACT

A new type of fluorescent gold nanoclusters (MU-Au NCs) was prepared by hydrothermal synthesis method using ammonium benzoate murexide (MU) as reducing agent and protecting agent.The synthesis method was simple and rapid.Based on the fluorescence quenching ability of spermine, a turn off type fluorescence analysis method was established for rapid and ultra sensitive detection of spermine.The linear range for detection of spermine was 0.003-300 μmol/L and the detection limit was 1 nmol/L (S/N=3).The established analytical method of spermine provided theoretical basis and reference for construction of spermine biosensor and actual sample detection.

17.
Article in Chinese | WPRIM | ID: wpr-611034

ABSTRACT

Objective To investigate the correlation between Helicobacter pylori (Hp) infection and blood lipid metabolism and atherosclerosis.Methods From October 2015 to October 2020,200 cases of Hp positive subjects were observed as observation group and 200 cases of Hp negative subjects as the control group,all subjects were blood lipid levels of vascular ultrasound and other related checks.Results The levels of total cholesterol (TC) (5.68± 1.46 mmol/L),triglyceride (TG) (1.58 ± 0.76 mmol/L) and low density lipoprotein cholesterol (LDL-C),and the difference was statistically significant (t=6.12,7.69,5.64,all P<0.05).The detection rate of carotid atherosclerotic plaque in the observation group was 66.00 %,which was significantly higher than that in the control group (49.50%),the difference was statistically significant (x2=29.61,P<0.05),and the observation group.The detection rate of stable plaque was 44.5 %,which was significantly higher than that of the control group 22.5%,the difference was statistically significant (x2 =21.73,P<0.05).Conclusion Hp infection can affect the level of lipid metabolism in patients,which is an important factor in the occurrence and development of atherosclerosis.

18.
Modern Clinical Nursing ; (6): 55-58, 2017.
Article in Chinese | WPRIM | ID: wpr-606914

ABSTRACT

Objective To study the relationship between psychological resilience and occupational stress of nurses in disinfec-tion supply center. Method A cluster sampling method was used to investigate 387 nurses in the supply and demand center by using the Chinese version of the Connor-Davidson scale (Connor-Davidson resilience, CD-RISC) and the nurse job stressors scale. Results In the disinfection supply center, the nurses' mental resilience score was (76.72±13.67), at the middle level. The total score on occupa-tion pressure was (72.84±12.19), at the moderate stress level. Pearson correlation analysis showed that the sterilization and supply cen-ter nurses' mental resilience score, together with the dimensions were positively correlated with occupation pressure. The mental re-silience and occupation pressure were positively correlated (P<0.05). Conclusions The psychological toughness and occupational stress of nurses in the sterilization and supply center are in the middle level. The nursing managers can promote the sustainable develop-ment of the nurses' psychological toughness through the stress management training and the nursing profession quality improvement.

19.
Article in Chinese | WPRIM | ID: wpr-667252

ABSTRACT

Objective To observe the serum level of cyclopropaneoctanoic acid 2-hexyl (CPOA2H) in patients with hypertrilyceridemia-related disorders,and investigate its clinical significance.Methods 53 obese patients (Obese group),62 obese patients after a 3 month low calorie diet (Obese patients after low calorie diet group),46 patients with chronic kidney disease (CKD group),and 60 healthy controls (Control group) were collected from Mar 2013 to Dec 2015 in Shaanxi Provincial People's Hospital.Gas chromatography-mass spectrometry (GC-MS) were used to detect the serum fatty acid and CPOA2H level in four groups.Results Ages,body mass index (BMI),C-reaction protein (CRP),total cholesterol (TC),triacylglycerols (TAG) among four groups had significant difference (F=6.85 ~ 25.36,P<0.05).Most saturated fatty acid (14 ∶ 0,16 ∶ 0) and monounsaturated fatty acid (14 ∶ 1,16 ∶ 1,18 ∶ 1,20 ∶ 1) in obese group were higher than controls (F=3.251~7.351,P<0.05).The polyunsaturated fatty acid (18 ∶ 2n-6,20 ∶ 4n-6) in CKD group were lower than controls (F=4.351 ~6.251,P<0.05).There existed significant difference of serum levels of CPOA2H among four group (F=19.95,P=0.005).Serum level of CPOA2H in control (r=0.63,P=0.033) and CKD group (r=0.61,P=0.044) were positively related with TAG.Serum level of CPOA2H in obese group and obese patients after low calorie diet group were positively related with TC (r=0.70,P=0.011;r=0.48,P=0.021) and TAG (r=0.75,P=0.024;r=0.72,P=0.018).Conclusion Hypertrilyceridemia-related disorders,such as obesity and CKD,presented with elevated serum levels of CPOA2H,it suggested that serum level of CPOA2H is positively related to serum TAG concentration rather than BMI.

20.
Article in Chinese | WPRIM | ID: wpr-663444

ABSTRACT

Objective To analyze the correlation between pathological of non small cell lung cancer(NSCLC)and mutations of the epidermal growth factor receptor(EGFR)gene.Methods Selected 96 patients with lung cancer for EGFR mutation de-tection in Shaanxi Provincial People's Hospital from January 2014 and April 2015,comparison of different types of NSCLC EGFR mutation rate.Results Gene mutation sensitive in 24 cases,in 12 cases and location of the mutation L858R,10 cases of mutation at position 19DEL,1 cases of mutation position G719X and S768I,1 cases EML4-ALK fusion.Mutation rate of 29.17%.Slightly lower than the international and domestic NSCLC epidermal growth factor receptor gene mutation sensitive rate of 30% of the average.Conclusion Target to treatment is aimed at the effective means for the treatment of NSCLC pa-tients with individualized,has reliable efficacy,less toxicity and adverse reactions,will become one of the most promising treatment,the study results below average,less attention to the base of the study population and need to raise awareness of routine testing.

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